CN113521120A - Ginseng and Ji fruit extracting solution and preparation process thereof - Google Patents

Ginseng and Ji fruit extracting solution and preparation process thereof Download PDF

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CN113521120A
CN113521120A CN202110875627.1A CN202110875627A CN113521120A CN 113521120 A CN113521120 A CN 113521120A CN 202110875627 A CN202110875627 A CN 202110875627A CN 113521120 A CN113521120 A CN 113521120A
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于永涛
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Abstract

The invention provides a ginseng and ji fruit extracting solution and a preparation process thereof, and relates to the technical field of plant extraction. The invention provides a preparation process of a Shenji fruit extracting solution, which can not only improve the extraction rate of active substances in the Shenji fruit, but also avoid the damage to the structure of the active substances and ensure the pharmacological effect of the Shenji fruit extracting solution. The invention also provides a Shenji fruit extract which has the pharmacological effects of obviously reducing blood fat and blood sugar, balancing blood pressure, resisting cancer and the like.

Description

Ginseng and Ji fruit extracting solution and preparation process thereof
Technical Field
The invention relates to the technical field of plant extraction, and particularly relates to a ginseng and gimeracil fruit extracting solution and a preparation process thereof.
Background
The plants contain various active substances, such as terpenoids, aromatic compounds, aliphatic compounds, nitrogen-containing and sulfur-containing compounds and the like, and the active substances have various functional properties, such as oxidation resistance, bacteriostasis, cancer resistance, tumor inhibition, mosquito repelling and insect prevention and the like. However, the plants contain many impurities which are not digested by human body, and the impurities can bring a part of active substances out of the body, so that the efficiency of the active substances absorbed and utilized by the human body is high. In order to increase the availability of active substances, plant active substances are often extracted to increase the purity of the active substances. Among the plant extraction methods, the steam distillation method is widely used due to the advantages of simple equipment, simple operation, low extraction cost and the like, but the higher temperature of the steam distillation method easily causes thermal decomposition and hydrolysis of a target substance, thereby causing the failure of part of active substances.
Disclosure of Invention
The invention aims to provide a preparation process of a Shenji fruit extracting solution, which can not only improve the extraction rate of active substances in the Shenji fruit, but also avoid the damage to the structure of the active substances and ensure the pharmacological effect of the Shenji fruit extracting solution.
The invention also aims to provide a Shenji fruit extract which has the pharmacological effects of obviously reducing blood fat and blood sugar, balancing blood pressure, resisting cancer and the like.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
On one hand, the preparation process of the ginseng and Jiguo extracting solution provided by the invention comprises the following steps: adding ethanol into Ginseng radix fructus Jujubae, reflux-extracting at 70-80 deg.C, separating filtrate and residue, reflux-extracting the residue twice according to the above conditions, and mixing the three filtrates to obtain mixed solution; distilling the mixed solution at 45-50 deg.C under reduced pressure, centrifuging, and discarding the lower layer to obtain emulsion; adsorbing the emulsion by adopting an adsorbing material, and eluting by using ethanol to obtain an active solution; distilling the active solution at 45-55 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae.
On the other hand, the invention also provides a ginseng and Ji fruit extracting solution which is prepared by the preparation process of the ginseng and Ji fruit extracting solution.
The ginseng and Ji fruit extracting solution and the preparation process thereof disclosed by the embodiment of the invention have the following beneficial effects that:
the preparation process of the ginseng fruit provided by the invention selects ethanol as an extraction solvent of the ginseng fruit, and extracts active substances in the ginseng fruit by low-temperature heating. In addition, the extract extracted by the ethanol has high yield, and the ethanol is non-toxic and non-corrosive, can be recycled under reduced pressure and can be repeatedly used, so the method is suitable for being used as the extraction solvent of the ginseng fruit. The filter residue obtained in the above one procedure is used as a new raw material for reflux extraction, so that residual substances in the filter residue can be extracted, the richness of active substance composition is increased, the extracting solution is closer to the active ingredients in the natural ginseng fruit, and the physiological effect of the active substances is improved. Mixing the filtrates to obtain mixed solution, distilling at 45-50 deg.C under reduced pressure to obtain crude extractive solution, and purifying at relatively low temperature by reduced pressure distillation to obtain radix Ginseng and fructus Rosae Davuricae with fragrance and active substance physiological function similar to natural ones. Not only can keep the savory and mellow smell of the ginseng fruit, but also can keep the physiological characteristics of the ginseng fruit, and furthest keeps the efficacy of active substances. Then the crude extract is centrifugally separated to obtain emulsion, so that impurities in the dissolved matter can be removed, active ingredients of the ginseng fruit are retained to the maximum extent, and the utilization rate of a human body is improved. Then, adsorbing the emulsion by adopting an adsorbing material so as to achieve the aims of further purifying and decontaminating the emulsion; then the active liquid is obtained by ethanol elution, and the ethanol is taken as an elution solvent so as to be convenient for phase separation with the active substance in the later period and improve the purity of the active substance. Distilling the active solution at 45-55 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae. The process purifies and concentrates the active substances in the Jijiguo extracting solution in a first-level manner, so that the physiological activity of the active substances is reserved, the failure of the activity caused by the damage of the structure of the active substances by high temperature is avoided, the content of the active substances in unit volume is increased, and the effect of the physiological performance is improved.
On the other hand, the extract of the ginseng fruit, which is prepared by the preparation process of the extract of the ginseng fruit, contains more active substances and more abundant components, and improves the bioavailability of the ginseng fruit. The cucurbitacin component contained in the herba senecionis extract is a highly-oxidized tetracyclic triterpenoid extracted from plants, and the cucurbitacin B, E is the main component, and has various biological activities such as anti-tumor, anti-inflammatory and the like. The extract of the ginseng and the fructus lycii also contains gypenoside and ginseng total saponin, and the pharmacological actions of the gypenoside and the ginseng total saponin are similar, so that the ginseng and the fructus lycii have the pharmacological actions of obviously reducing blood fat, reducing blood sugar, balancing blood pressure, resisting cancer and aging, protecting liver, improving immune function and the like. Therefore, the ginseng and ji fruit extracting solution can be applied to the fields of resisting tumors, reducing blood fat and blood sugar, preventing aging and the like.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.
The invention provides a preparation process of a ginseng and gimbals fruit extracting solution, which comprises the following steps: adding ethanol into Ginseng radix fructus Jujubae, reflux extracting at 70-80 deg.C, and filtering to obtain residue and filtrate; then, taking the filter residue obtained in the previous reflux extraction as a raw material, and extracting according to the reflux extraction steps for three times in total; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 45-50 deg.C under reduced pressure to obtain crude extractive solution; centrifuging the crude extract, and only taking the upper and middle layer solution to obtain emulsion; adsorbing the emulsion by adopting an adsorbing material, and eluting by using ethanol to obtain an active solution; distilling the active solution at 45-55 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae.
In detail, the invention selects ethanol as the extraction solvent of the ginseng fruit, and the active substances are extracted from the ginseng fruit by a series of physical processes of permeation, dissolution, distribution, diffusion and the like of the ethanol in the ginseng fruit. In detail, the feed-liquid ratio of the ginsengii fruit to the ethanol is 1 (8-12), the feed-liquid ratio can ensure that the ginsengii fruit is fully contacted with the ethanol, so that active substances are dissolved out more quickly, raw materials are wasted when the feed-liquid ratio is too large, and the extraction rate of the active substances is reduced when the feed-liquid ratio is too small, so that the bioavailability is reduced. Further, in order to sufficiently dissolve the active substances in the Jiyu fruit, the Jiyu fruit can be crushed into particles of 180-220 meshes and then added with ethanol. The ginseng fruit is crushed into small particles, so that the contact area of ethanol and the ginseng fruit particles can be increased, the dissolution rate of active substances is accelerated, the extraction time is shortened, and the extraction rate of the active substances of the ginseng fruit is improved.
Reflux extracting mixture of ethanol and radix Ginseng and fructus Jujubae at 70-80 deg.C. Wherein, the movement speed of molecules can be accelerated by the temperature rise, and the permeation, diffusion and dissolution speed of the molecules can be accelerated by the structural change of cell membranes caused by the high temperature. However, since the temperature is too high, the structure of part of the active material is destroyed, and the ethanol is easily volatilized, which is not favorable for extraction of the active material, and the extraction amount is reduced, the temperature of the reflux extraction is preferably 70 to 80 ℃.
In detail, 70-90 v/v% ethanol is used in reflux extraction. According to the principle of similar solubility, the extraction amount of active substances gradually increases with the increase of ethanol concentration. However, when the concentration is more than 80 v/v%, the water content in the ethanol solvent is reduced, the polarity of the solvent is weakened, which is disadvantageous for the extraction of the ginseng fruit, and the extraction rate of the active substance is lowered. Therefore, it is reasonable to select an ethanol concentration of 70-90 v/v%.
Further, the time for reflux extraction is 70-90 minutes. The longer the reflux time, the more beneficial the extraction of the active substance. However, the reflux time is too long, and the active material is affected by the temperature, thereby causing a decrease in the extraction rate of the active material. Therefore, the reflux time is too long to facilitate the extraction of the active substance, so the reflux time is selected to be 70-90 minutes.
After the active substances in the ginseng and fruit are extracted by the ethanol, a plurality of active substances still remain in the filter residue, the filter residue is taken as the raw material, the secondary extraction is carried out by the ethanol according to the reflux extraction step, and the secondary filter residue is taken as the raw material for carrying out the tertiary extraction. Therefore, the residual substances in the filter residue can be extracted, the richness of the active substance composition is increased, the extracting solution is closer to the effective components in the natural ginseng fruit, and the physiological effect of the active substances is improved.
Mixing the filtrates to obtain a mixed solution, and distilling at 45-50 deg.C under reduced pressure to obtain a crude extractive solution. The reduced pressure distillation can purify the ginseng fruit at a relatively low temperature, and the fragrance and the physiological function of the active substances of the ginseng fruit are more similar to those of natural ginseng fruit. Not only can keep the savory and mellow smell of the ginseng fruit, but also can keep the physiological characteristics of the ginseng fruit, and furthest keeps the efficacy of active substances.
The crude extract is centrifugally separated, only the solution of the upper layer and the solution of the middle layer are reserved, dissolved impurities in the lower layer can be removed, active ingredients of the ginseng fruit are reserved to the maximum degree, and the utilization rate of a human body is improved. In detail, the centrifugal rotating speed is 7500-8500r/min, and the centrifugal time is 8-15 minutes. The liquid-liquid separation is realized by utilizing the principle that components with different emulsion densities rapidly settle and separate in a centrifugal force field. Under the condition of centrifugal separation, impurity substances dissolved out from the crude extraction liquid and active substances can be separated from each other, and the purity of the active substances is improved.
And then, adsorbing the emulsion by using an adsorbing material, and further purifying and decontaminating the emulsion. In detail, the adsorbing material in the invention is macroporous adsorption resin, and the macroporous adsorption resin is a separation material combining the adsorption and screening principles. The adsorption of the macroporous adsorption resin is essentially a surface adsorption phenomenon generated by high dispersion of objects or uneven acting force on surface molecules, and the adsorption performance is caused by Van der Waals attractive force or hydrogen bond generation; meanwhile, due to the porous structure of the macroporous adsorption resin, the macroporous adsorption resin has a screening effect on substances with different molecular sizes. The invention adopts AB-8 type macroporous absorption resin, and absorbs the emulsion with the volume flow of 1.3-1.8 BV/h. The AB-8 type macroporous adsorption resin can adsorb a large amount of active substances in a short time under the volume flow, and has high desorption rate and high desorption speed. The macroporous absorption resin is eluted by ethanol to achieve the purpose of separating active substances, and then the active substances are purified and purified. Further, after the emulsion is adsorbed by an adsorbing material, 55-65 v/v% ethanol is adopted for elution to obtain active liquid. The ethanol is used as eluent, the volume fraction of the ethanol is too low or too high, the polarity of the ethanol is greatly different from that of a target compound, the chemical bond formed by the resin and the active substance cannot be damaged, and other adsorbed impurities compete for desorption to reduce the elution rate, so 55-65 v/v% of ethanol is adopted.
Then distilling the active liquid at 45-55 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae. The process can retain the physiological activity of the active substance, and concentrate the active substance, so that the content of the active substance in unit volume is increased, and the physiological performance and efficacy of the active substance are improved. Further, before the active liquid is distilled under reduced pressure, the active liquid is treated with an ion exchange resin to remove salts and pigments in the active liquid. When the active substances are extracted from the ginseng fruit, a part of impurities such as pigments, salts and the like are often mixed in the solution of the ginseng fruit. Wherein the pigment is mainly derived from pigment contained in the cellulose raw material, and products generated by caramelization reaction, Maillard reaction and the like. During the extraction process of the ginseng fruit, a large amount of sodium ions are often mixed into the extracting solution, and the quality of the ginseng fruit extracting solution serving as a final product is reduced due to the existence of the sodium ions. When the extract is used as an additive to be added into other substances, the salts and the pigments can cause the problems of demulsification, deepening color and the like of a final product, and the application of the ginseng fruit extract is limited. The ion exchange resin is a high molecular compound with a network structure of functional groups, and the ion exchange resin has high purity of the obtained product due to simple separation technology and equipment, convenient operation and high production continuity degree. In the invention, cation exchange resin is adopted to desalt desorption solution, and anion exchange resin is adopted to decolor desorption solution.
The invention also provides the extracting solution of the ginseng and ji fruit, and the extracting solution of the ginseng and ji fruit prepared by the preparation process of the extracting solution of the ginseng and ji fruit contains more active substances and more abundant components, thereby improving the bioavailability of the ginseng and ji fruit. The cucurbitacin component contained in the herba senecionis extract is a highly-oxidized tetracyclic triterpenoid extracted from plants, and the cucurbitacin B, E is the main component, so that the herba senecionis extract has various biological activity functions of resisting tumors, resisting inflammation and the like. The extract of the ginseng and the fructus lycii also contains gypenoside and ginseng total saponin, and the pharmacological actions of the gypenoside and the ginseng total saponin are similar, so that the ginseng and the fructus lycii have the pharmacological actions of obviously reducing blood fat, reducing blood sugar, balancing blood pressure, resisting cancer and aging, protecting liver, improving immune function and the like. Due to the pharmacological characteristics of the Shenji fruit extract, the Shenji fruit extract can be used as an additive and widely applied to the fields of medicinal preparations, beverages, skin care products and the like, and has wide application prospect.
Example 1
The embodiment provides a manufacturing process of a ginseng and ji fruit extracting solution, which comprises the following steps:
crushing the ginseng fruit into 200-mesh particles; adding 75 v/v% ethanol into the Shenjiguo granules, performing reflux extraction at 80 ℃ for 80 minutes, and filtering after extraction to respectively obtain filter residues and filtrate; then, taking filter residues obtained in the last reflux extraction as raw materials, and extracting according to the reflux extraction steps for three times; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 50 deg.C under reduced pressure to obtain crude extractive solution; centrifuging the crude extract, and taking the upper and middle layer solutions to obtain emulsion; adsorbing the emulsion by macroporous adsorption resin at a volume flow of 1.5BV/h, and eluting by 60 v/v% ethanol to obtain an active solution; performing resin exchange treatment on the active liquid; then distilling the extract at 50 ℃ under reduced pressure until no ethanol exists, and obtaining the ginseng and fructus gigantis extract.
It is to be noted that the feed-liquid ratio of the ginseng fruit and the ethanol is 1:10, the centrifugal speed is 8000r/min, and the centrifugal time is 10 minutes.
Example 2
The embodiment provides a manufacturing process of a ginseng and ji fruit extracting solution, which comprises the following steps:
crushing the ginseng fruit into 180-mesh particles; adding 70 v/v% ethanol into the Shenji fruit granules, performing reflux extraction at 75 ℃ for 90 minutes, and filtering after extraction to respectively obtain filter residues and filtrate; then, taking filter residues obtained in the last reflux extraction as raw materials, and extracting according to the reflux extraction steps for three times; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 45 deg.C under reduced pressure to obtain crude extractive solution; centrifuging the crude extract, and taking the upper and middle layer solutions to obtain emulsion; adsorbing the emulsion by adopting an adsorbing material at a volume flow of 1.3BV/h, and eluting by 65 v/v% ethanol to obtain an active solution; performing resin exchange treatment on the active liquid; then distilling the extract at 45 ℃ under reduced pressure until no ethanol exists, and obtaining the ginseng and fructus gigantis extract.
The ratio of the ginseng fruit to the ethanol is 1:8, the centrifugal speed is 8500r/min, and the centrifugal time is 15 minutes.
Example 3
The embodiment provides a manufacturing process of a ginseng and ji fruit extracting solution, which comprises the following steps:
crushing the ginseng fruit into 220-mesh particles; adding 90 v/v% ethanol into the Shenjiguo granules, performing reflux extraction at 78 ℃ for 70 minutes, and filtering after extraction to respectively obtain filter residues and filtrate; then, taking filter residues obtained in the last reflux extraction as raw materials, and extracting according to the reflux extraction steps for three times; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 48 deg.C under reduced pressure to obtain crude extractive solution; centrifuging the crude extract, and taking the upper and middle layer solutions to obtain emulsion; adsorbing the emulsion by macroporous adsorption resin at a volume flow of 1.8BV/h, and eluting by 55 v/v% ethanol to obtain an active solution; distilling the active solution at 55 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae.
The ratio of the ginseng fruit to the ethanol is 1:12, the centrifugal speed is 7500r/min, and the centrifugal time is 8 minutes.
Example 4
The embodiment provides a manufacturing process of a ginseng and ji fruit extracting solution, which comprises the following steps:
adding ethanol into the ginseng fruit, carrying out reflux extraction for 74 minutes at the temperature of 73 ℃, and filtering after extraction to respectively obtain filter residue and filtrate; then, taking filter residues obtained in the last reflux extraction as raw materials, and extracting according to the reflux extraction steps for three times; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 47 deg.C under reduced pressure to obtain crude extractive solution; centrifuging the crude extract, and taking the upper and middle layer solutions to obtain emulsion; adsorbing the emulsion by adopting an adsorbing material at a volume flow of 1.5BV/h, and eluting by 61 v/v% ethanol to obtain an active solution; performing resin exchange treatment on the active liquid; then distilling at 52 deg.C under reduced pressure until no ethanol is obtained to obtain radix Ginseng and fructus Jujubae extractive solution.
Example 5
The embodiment provides a manufacturing process of a ginseng and ji fruit extracting solution, which comprises the following steps:
adding ethanol into the Shenji fruit, performing reflux extraction at 76 ℃, and filtering after extraction to respectively obtain filter residue and filtrate; then, taking filter residues obtained in the last reflux extraction as raw materials, and extracting according to the reflux extraction steps for three times; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 48 deg.C under reduced pressure to obtain crude extractive solution; centrifuging the crude extract, and taking the upper and middle layer solutions to obtain emulsion; adsorbing the emulsion by using macroporous adsorption resin, and eluting by using ethanol to obtain active liquid; distilling the active solution at 54 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae.
Example 6
The embodiment provides a manufacturing process of a ginseng and ji fruit extracting solution, which comprises the following steps:
adding ethanol into the Shenji fruit, performing reflux extraction at 77 ℃, and filtering after extraction to respectively obtain filter residue and filtrate; then, taking filter residues obtained in the last reflux extraction as raw materials, and extracting according to the reflux extraction steps for three times; mixing the filtrates obtained in the three times to obtain a mixed solution; distilling the mixed solution at 49 ℃ under reduced pressure to obtain a crude extract; centrifuging the crude extract, and taking the upper and middle layer solutions to obtain emulsion; adsorbing the emulsion by adopting an adsorbing material, and eluting by using ethanol to obtain an active solution; distilling the active solution at 47 deg.C under reduced pressure until no ethanol is present to obtain extractive solution of radix Ginseng and fructus Jujubae.
Comparative example
Extracting radix Ginseng and fructus Tribuli by conventional steam distillation method for 80 min at a material-to-liquid ratio of 1: 10.
Test example 1
Respectively further drying the extract of radix Ginseng and fructus Girardiniae Palmatae obtained in examples 1-6 and comparative example to obtain extract-like purified product, and weighing mass as M1The original quality of the ginseng fruit is recorded as M0And calculating according to the following formula of extraction rate: the extraction yield is M1/M0100%, see table 1 for specific results.
TABLE 1 extraction of Ginseng radix et rhizoma Rhei fruit for each sample
Figure BDA0003190159060000101
As can be seen from the data in table 1, the extraction rate of the active substance of ginseng fruit in the extract liquid obtained in example 1 of the present invention reached 9.43%, while the extraction rate of the active substance of ginseng fruit extracted by the method of the comparative example was only 4.40% at the same extraction time and extraction temperature, which indicates that the extraction rate of the active substance can be improved by the process for preparing the extract liquid of ginseng fruit according to the present invention. And the extraction rate of the ginseng fruit obtained in other embodiments is over 9 percent, so that the bioavailability of active substances of the ginseng fruit is improved.
Test example 2
Measuring the cucurbitacin content: further drying the extract of the ginseng fruit to obtain an extractum purified product, precisely weighing 4.0mg of the purified product, dissolving the purified product with 70% ethanol solution to a constant volume of 10m L to prepare a test solution with the concentration of 0.4mg/m L, and filtering the test solution with a filter membrane of 0.45 mu m for later use. The purity of cucurbitacin in the purified solution is observed by adopting high performance liquid chromatography, a chromatographic column adopts C18(4.6mm multiplied by 200mm, 5 mu m), a mobile phase is methanol-water (60:40), and the flow rate: 1.0m L/min, detection wavelength: 230nm, column temperature: 25 ℃, sample introduction: 20 μ L was measured. The cucurbitacin B reference substance (purity not less than 98.67%) was purchased from Kyorman Tech technologies, Inc.
As is clear from the test in test example 1, the extract of sangji berries obtained in example 6 of the present invention has the least active ingredient, and therefore, the extract was used as a test sample and tested by the method for measuring the content of cucurbitacin described above to determine the content of cucurbitacin B in the active substance.
The test is carried out according to the test method, the content of cucurbitacin B in the obtained ginseng and jejunum fruit extracting solution is 10.6mg/g, which shows that the ginseng and jejunum fruit extracting solution has cucurbitacin B, and the cucurbitacin B has various biological activity functions of resisting tumors, resisting inflammation and the like, and can obviously reduce blood fat, reduce blood sugar, balance blood pressure, resist cancers, resist aging, protect liver, improve immunologic function and other pharmacological actions. Therefore, the ginseng and ji fruit extracting solution can be applied to the fields of resisting tumors, reducing blood fat and blood sugar, preventing aging and the like.
In conclusion, the preparation process of the extract of the ginseng fruit provided by the invention can improve the extraction rate of active substances in the ginseng fruit, avoid the damage to the structure of the active substances and ensure the pharmacological effect of the ginseng fruit. The extract of the ginseng and the jejunum fruits provided by the invention has the pharmacological effects of obviously reducing blood fat and blood sugar, balancing blood pressure, resisting cancer and the like.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims (10)

1. The preparation process of the ginseng and ji fruit extracting solution is characterized by comprising the following steps:
adding ethanol into Ginseng radix fructus Jujubae, reflux-extracting at 70-80 deg.C, separating filtrate and residue, reflux-extracting the residue twice according to the above conditions, and mixing the three filtrates to obtain mixed solution; distilling the mixed solution at 45-50 ℃ under reduced pressure, centrifuging, and removing the lower layer liquid to obtain emulsion; adsorbing the emulsion by using an adsorbing material, and eluting by using ethanol to obtain an active solution; distilling the active solution at 45-55 deg.C under reduced pressure until no ethanol is contained to obtain extractive solution of radix Ginseng and fructus Jujubae.
2. The process for preparing the extracting solution of radix scrophulariae and fructus jequiriae as claimed in claim 1, wherein in the step of reflux extraction, the ratio of the radix scrophulariae and fructus jequiriae to the ethanol is 1 (8-12).
3. The process for preparing the extracting solution of red ginseng and fruit according to claim 1, wherein the reflux extraction is performed for 70-90 minutes.
4. The process for preparing the extracting solution of ginseng fruit according to claim 1, wherein 70-90 v/v% ethanol is used in the step of reflux extraction.
5. The process for preparing extracting solution of radix Ginseng and fructus Jujubae as claimed in claim 1, wherein in the step of centrifugation, the centrifugation speed is 7500-8500r/min, and the centrifugation time is 8-15 min.
6. The process for preparing the extracting solution of ginseng fruit according to claim 1, wherein the adsorbing material is AB-8 type macroporous adsorption resin, and the emulsion is adsorbed at a volume flow rate of 1.3-1.8 BV/h.
7. The process for preparing the extracting solution of radix scrophulariae and fructus jequiriti according to claim 1, wherein the emulsion is subjected to adsorption treatment and then is eluted by 55-65 v/v% ethanol to obtain an active solution.
8. The process for producing the extract of red ginseng fruit according to claim 1, wherein the active solution is treated with an ion exchange resin before the active solution is distilled under reduced pressure.
9. The process for preparing the extracting solution of sangji fruit as claimed in claim 1, wherein the step of reflux-extracting the sangji fruit is preceded by the step of crushing the sangji fruit into particles of 180-220 meshes for reflux-extracting.
10. An extract of ginseng ji fruit, characterized in that it is produced by the process for producing an extract of ginseng ji fruit according to any one of claims 1 to 9.
CN202110875627.1A 2021-07-30 2021-07-30 Ginseng and Ji fruit extracting solution and preparation process thereof Pending CN113521120A (en)

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Publication number Priority date Publication date Assignee Title
CN114698842A (en) * 2022-05-21 2022-07-05 于永涛 Ginseng-pilose antler-fruit composition for nourishing and protecting liver and preparation method thereof

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CN113080056A (en) * 2021-05-24 2021-07-09 于永涛 Cultivation method of ginseng and Ji fruit

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