KR20140052286A - Cell culture factory incubator for gas inlet control means - Google Patents

Abstract

The present invention relates to a cell-culturing incubator having a gas inlet control means. A heat flow path including an external cover that blocks external heat and an internal cover is formed in order to precisely maintain the inner temperature of the large cell-culturing incubator. An HEPA filter and a capsule-type filter are prepared in order to prevent impurities from being injected in the cells accommodated therein. Multiple gas sensors having different functions are prepared to be automatically operated by measuring the concentration of gas. A cart means is prepared to be simultaneously dislodged and rotated so that cells are easily discharged. The inner temperature of a heat flow path culturing chamber including the external cover filled with insulating material for blocking external heat and the internal cover separated from the inside of the external cover is precisely maintained. The HEPA filter is prepared on the front surface of a heat flow fan therein so that impurities are prevented from being injected in cells accommodated therein. A temperature sensor is fixed to a desired position so that temperature is simply measured. A regulator is automatically operated according to set values. The concentration of gas therein is easily measured when a gas sensor equipped therein is broken. Pure gas is supplied into the culturing chamber. A culturing plate cart means is prevented from being dislodged and is moved by means of the rotation of wheels at the same time.

Description

Description of the Related Art [0002] Cell culture factory incubators for gas inlet control means equipped with a gas flow-

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an incubator for culturing a culture such as a cell or a microorganism, and more particularly, to an incubator in which an outer cover for blocking external heat and a heat- A plurality of gas sensors having different functions for automatically operating by measuring a gas concentration are provided, and a plurality of gas sensors are provided for discharging the cell culture plate The present invention relates to a cell culture incubator provided with a gas inflow control means provided with a cart means for convenience.

Generally, since an incubator (a culture medium) is to cultivate a culture (sample) such as a cell or a microorganism as an object to be cultured by keeping the temperature (temperature in the culture chamber) or the CO2 concentration constant and making the inside sterile, A periodic sterilization treatment is required.

Also, the heating heater and its control device for controlling the temperature in the culture room (storage chamber) may be replaced with a gas supply means for supplying and controlling a supply gas such as CO 2 or O 2 to the culture chamber for controlling the gas concentration, And a multi-gas incubator in which a sample is cultured in a culture chamber.

On the other hand, as disclosed in Patent Document 1 (Japanese Unexamined Patent Publication (Kokai) No. 7-31462), in order to uniformly and efficiently heat the inside of the inner box and improve its temperature returning property, There is provided a culture apparatus having an inner box disposed inside of an outer box and a heating device attached to the inner box in such a manner as to be in contact with the inner box to heat the inner box, And to form an air layer (air jacket) for heat insulation of the outside and the culture room and for promoting heat transfer by natural convection.

In particular, as described in Patent Document 2 (Japanese Unexamined Patent Publication (Kokai) No. 2000-166536), a humidification dish for storing humidification water for humidification control in a culture room is provided at the bottom of the culture chamber.

Here, FIG. 1 is a configuration diagram showing a conventional cell culture incubator, which is disclosed in Patent Document (Korean Patent Laid-Open Publication No. 2005-0037959).

1, a heat insulating box body having an opening in a front surface thereof, a heat insulating door which is openably and closably attached to the heat insulating box body, a transparent inner door which closes the opening so as to open and close, And a culture chamber surrounded by a heat insulating box main body for culturing a sample such as a cell or a microorganism. The heat insulating box main body is composed of a metal outer box, a metal inner box, and an inner box And an air layer disposed on the inner side of the heat insulating material.

However, in the conventional cell culture incubator, since the cell culture plates are stacked in multiple stages and the cells must be directly loaded by the user, there is a problem in that it is very inconvenient to use, and external apparatuses for checking the concentration of the gas flowing into the culture chamber, There is a problem that it is inconvenient that the user adjusts the gas concentration by hand measurement.

SUMMARY OF THE INVENTION The present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to provide an apparatus and a method for controlling an amount of gas in an incubator, The HEPA filter is installed at the front of the heat flow fan inside to precisely maintain the internal temperature of the culture chamber and prevents the impurities from flowing into the cells housed therein and fixing the temperature sensor at a desired position, And a gas inflow controlling means for inserting or discharging the culture plate safely with diagonal driving wheels at a lower portion of the culture plate cart means.

In order to accomplish the above object, a cell culture incubator equipped with a gas inflow control means according to the present invention comprises a main body having a plurality of culture chambers accommodating therein a cell culture plate, a plurality of doors And a control box part formed on one side of the main body part, wherein culture plate cart means for inserting or discharging the cell culture plate into the culture chamber is formed; The control box part is provided with a touch screen panel for controlling the operation of the cell culture incubator, and on the rear surface thereof, a leakage preventing circuit breaker for preventing electric leakage, a temperature stabilizer for safety inspection of temperature, a temperature recorder for displaying temperature, And a regulator connected to the inside of the gas inlet and regulating and introducing a gas amount; A cart rail section having a trapezoidal shape is formed at a lower portion of the culture chamber so that the culture plate cart means is moved by a predetermined movement path; A plurality of temperature sensors connected to the temperature recorder and connected to the temperature cable, the inner temperature of which is not measured so as to be moved to a predetermined position, A gas sensor including a first gas sensor for measuring the internal gas concentration of the culture chamber and a second gas sensor for measuring the internal gas concentration of the culture chamber and outputting a control signal for the operation of the regulator, .

An inner cover formed to be spaced apart from the outer cover by a predetermined distance; and a space formed between the inner cover and the outer cover to allow heat to flow therebetween And a heat flow furnace.

A plurality of coil heaters for generating heat are attached to a side portion and an upper inner side surface of the inner cover and a coil heater portion for generating heat is attached to the inner surface of the inner cover, And a heat flow fan connected to the fan motor to circulate the heat generated in the pipe heater.

A fan cover is formed on a rear surface of the inner cover to cover a front surface of the heat-flow fan, and a heat-absorbing passage through which heat is introduced is formed in a central portion of the fan cover, And a filter unit is provided to filter out foreign matter distributed in heat and gas.

The culture plate cart means includes a culture plate insertion portion including a plurality of insertion support rods into which the cell culture plate is inserted so that the cell culture plate is mounted in multiple layers, And a plurality of rail wheel portions.

Wherein the rail wheel portion comprises a wheel shaft inclined outward to be seated on the cart portion of the trapezoidal shape and a wheel which is recessed and rotated on the wheel shaft; And a wheel inclined portion is formed on an outer circumferential surface of the wheel so as to be seated on an inclined surface of the trapezoidal cart portion.

A gas supply pipe connected to one end of the regulator for supplying gas to the culture chamber is formed in a side surface of the culture chamber and a supply port for supplying gas to the culture chamber is formed at an end of the gas supply pipe, And a return port for recovering the supplied gas is formed in the inner space.

And a sampling port connected to the culture chamber to be connected to an external meter reading device for arbitrarily supplying other kinds of gas from the outside or measuring an external gas concentration is provided on the rear surface of the control box portion.

And a capsule type gas filter unit is formed in a part of the gas supply pipe or the sampling port so as to filter impurities distributed in the gas.

As described above, the cell culture incubator equipped with the gas inflow control means according to the present invention has the following effects.

First, by forming an outer cover filled with a heat insulating material for blocking external heat, a heat flow path is formed by an inner cover formed to cut off the temperature of the outside and to be spaced by a predetermined distance from the inside of the outer cover, The cell culturing operation can be performed precisely,

Second, a fan cover is provided on the rear surface of the inner cover, the fan cover being spaced apart by a plurality of bolts and having a heat intake path through which heat is introduced into the central portion. By including a hepafi filter in the heat intake path, Thereby preventing impurities from being introduced into the cells of the cell culture plate housed therein. In addition,

Third, by providing a temperature sensor that is not fixed to the inside of the culture chamber and connected to a temperature cable of a predetermined length, the temperature sensor can be easily moved to accurately measure the temperature at a desired position in the culture chamber, The temperature can be conveniently measured by fixing the temperature sensor at a desired position,

Fourthly, by providing two gas sensors different in purpose inside the culture room, it is possible to measure the concentration of the internal gas in the culture chamber by using one gas sensor and to check the internal gas concentration in the culture chamber by using a regulator It is possible to improve the convenience of the operation by automatically operating according to the predetermined numerical value,

Fifth, by forming a sampling port connected to the culture chamber on the rear surface of the control box part, it is possible to arbitrarily supply another kind of gas from the outside or to connect with an external meter reading device for measuring the concentration of the external gas, The gas concentration inside the city can be easily measured,

Sixth, since a capsule type gas filter unit is provided in a part of the gas supply pipe or the sampling port, the impurities distributed in the gas introduced from the outside are easily filtered to supply the clean gas to the inside of the culture chamber to perform the cell culture operation finely In addition,

Seventhly, a trapezoidal cart rail section is formed in the lower part of the culture chamber, and a wheel inclination section is formed on the outer circumferential surface of the wheel of the culture plate cart means to seat the wheel inclined section on the inclined surface formed on both sides of the cart reed, It is possible to work simultaneously with the rotation of the wheel.

1 is a view showing a cell culture incubator according to the prior art,
2 is a perspective view showing a cell culture incubator according to the present invention,
3 is a side view showing the inside of a cell culture incubator according to the present invention,
4 is a front view showing a cell culture incubator according to the present invention,
5 is a rear view showing a cell culture incubator according to the present invention.

Hereinafter, an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

2 to 5, the cell culture incubator equipped with the gas inflow controlling means according to the present invention comprises a main body 10 having a plurality of culture chambers 11 in which a cell culture plate P is housed, A plurality of door portions 20 opened and closed on the front surface of the main body 10 and a control box portion 40 formed on one side of the main body 10, A culture plate cart means 30 for inserting or discharging the cell culture plate P is formed inside the culture chamber 11; A touch screen panel 41 for controlling the operation of the cell culture incubator 1 is provided on the front surface of the control box unit 40. An electric leakage preventing circuit breaker 42 for preventing electric leakage is provided on the rear surface, And a regulator 46 which is connected to the inside of the gas inlet 45 and regulates the amount of the gas to be introduced into the gas inlet 45. The temperature regulator 44, ; A cartridges 12 of a trapezoidal shape are formed in a lower portion of the culture chamber 11 so that the culture plate cart means 30 is moved by a predetermined movement path; A plurality of temperature sensors 47 connected to the temperature recorder 44 and connected to the temperature cable 47a, the inner temperature of which is fixed and is not fixed so as to be moved to a predetermined position, is provided in the culture chamber 11; A first gas sensor 48a for measuring the concentration of the internal gas in the culture chamber 11 and a second gas sensor 48b for measuring the concentration of the internal gas in the culture chamber 11 and controlling the operation of the regulator 46, And a second gas sensor 48b for outputting the gas sensor 48b.

The cell culture plate P accommodated in the upper layer of the culture plate cart means 30 may include protrusions (not shown) or the like, which are coupled to the upper and lower surfaces of the cell culture plate P, It is preferable that a hole (not shown) is formed.

It is preferable that the touch screen panel 41 is installed in a buried form to prevent foreign matter such as dust from accumulating.

The cartridges 12 may be connected to the rear and front surfaces of the culture chamber 11 by bolts (not shown) so as to be attached to the lower portions of the plurality of culture rooms 11.

The temperature cable 47a is formed to have a length corresponding to the width or height of the culturing chamber 11 so as to accurately measure the temperature at any position inside the culturing chamber 11, It is preferable that attachment means (not shown) is provided on one side to facilitate the movement in the culture room 11.

The control box unit 40 may include a power connection unit (not shown) for connecting the main power source to the incubator.

The main body 10 includes an outer cover 13 formed to be able to block the outer surface except the front side thereof, an inner cover 14 spaced apart from the outer cover 13 by a predetermined distance, And a heat flow path (15) in which a space is formed between the inner cover (14) and the outer cover (13) to allow heat to flow therethrough.

Meanwhile, it is preferable that the outer cover 13 is filled with a heat insulating material (not shown) for blocking heat.

A pipe heater portion 16 for generating heat is attached to the inner bottom surface of the inner cover 14 and a plurality of coil heater portions And a heat flow fan 18 connected to the fan motor M for circulating heat generated from the coil heater unit 17 and the pipe heater unit 16 is attached to the rear surface of the inner cover 14 Respectively.

The fan motor M connected to the heat-flow fan 18 is provided with a sealing rotation means (not shown) on the rotary shaft of the fan motor M so as to prevent the external air from flowing into the fan motor M, As shown in Fig.

The pipe heater portion 16 and the coil heater portion 17 generate heat by using electrical resistance and the pipe heater portion 16 is attached to the lower surface of the inner cover 14 located at the lower portion The coil heater part 17 is preferably attached to both sides and both sides of the inner cover 14 located on the upper side and the upper side.

At this time, it is preferable that the pipe heater part 16 and the coil heater part 17 are attached by welding or bolting.

A fan cover 19 is formed on the rear surface of the inner cover 14 so as to cover the front surface of the heat flow fan 18 with a plurality of bolts B, A heat absorbing passage 19a through which heat is introduced is formed and the heat absorbing passage 19a is provided with a filter portion 19b for filtering foreign substances distributed in the heat and gas flowing into the heat absorbing passage 19a.

Here, the filter unit 19b is preferably used as a HEPA filter (not shown) which is excellent in filtering efficiency and can be used at a high temperature.

The culture plate cart means 30 includes a culture plate insertion portion 31 composed of a plurality of insertion support rods 31a into which the cell culture plate P is inserted so that the cell culture plate P is mounted in multiple layers, And a plurality of rail wheel portions 32 formed on the lower portion of the culture plate insertion portion 31 so that the culture plate cart means 30 is moved.

The rail wheel portion 32 includes a wheel shaft 32a inclined outward to be seated on the cart portion 12 of the trapezoidal shape and a wheel 32b that is inserted into the wheel shaft 32a and rotates Lt; / RTI > A wheel slope portion 32c is formed on an outer circumferential surface of the wheel 32b so as to be seated on an inclined surface of the trapezoidal cart portion 12.

Meanwhile, it is preferable that a bearing (not shown) is provided inside the wheel 32b so as to rotate in contact with the wheel shaft 32a.

A gas supply pipe 11a connected to one end of the regulator 46 for supplying gas to the culture chamber 11 is formed inside the side surface of the culture chamber 11, A feed port 11b for supplying a gas to the culture chamber 11 is formed at an end of the culture chamber 11 and a return port 11c for recovering the supplied gas is formed in the culture chamber 11.

The return port 11c is preferably provided with a return supply pipe (not shown) connected to the other end of the regulator 46 so that gas is circulated inside the culture chamber 11.

A sampling port (not shown) connected to the culture chamber 11 is connected to an external meter reading device (not shown) for arbitrarily supplying other types of gas from the outside or measuring an external gas concentration, 49 are provided.

In addition, a capsule type gas filter portion 11d is formed in a part of the gas supply pipe 11a or the sampling port 49 so as to filter impurities distributed in the gas.

The operation of the cell culture incubator having the gas inflow control means according to the present invention having the above-described structure will be described below.

As shown in FIG. 2, the cell culture incubator equipped with the gas inflow control means according to the present invention includes a plurality of door portions 20 provided to open and close the front surface of the main body portion 10, It is possible to prevent the gas from being discharged to the outside and to maintain the temperature and gas concentration necessary for the cell culture plate P accommodated therein.

Here, as shown in FIG. 3, the outer cover 13 filled with the heat insulating material (not shown) for blocking the external heat may block the outside temperature to maintain the internal temperature of the culture room 11, By forming the heat flow path 15 by the inner cover 14 formed so as to be spaced by a predetermined distance from the inside of the outer cover 13, it is possible to easily circulate the heat generated inside from the outside together with the outside temperature.

Heat can be generated in the lower portion of the culture room 11 due to the lower inner side surface of the inner cover 14 and the attached pipe heater portion 16. The side surface of the inner cover 14, Heat can be discharged from the front surface of the cultivation chamber 11 due to the plurality of coil heaters 17 attached to the inside of the culture chamber 11 and heat is circulated through the heat flow path 15, Can be blocked secondarily with the outer cover (13).

The heat generated by the coil heater part 17 and the pipe heater part 16 inside the culture chamber 11 is generated by the heat flow fan 18 connected to the fan motor M on the rear surface of the inner cover 14. [ So that the heat can be easily circulated.

The fan cover 19 is spaced apart from the rear surface of the inner cover 14 by a plurality of bolts B and has a heat suction path 19a through which heat is introduced into the central portion. The generated heat can be circulated in the direction of the arrow.

Also, it is possible to prevent the impurities contained in the refrigerant from being introduced into the heat-flow fan 18 while being circulated by the filter portion 19b provided in the heat-absorbing passage 19a.

3 to 5, the overall operation of the cell incubation incubator 1 can be operated by the touch screen panel 41 provided on the front surface of the control box unit 40, And can be operated in connection with the components described in Fig.

That is, when a short circuit occurs in the power supplied to the cell culture incubator (1) due to the earth leakage breaker (42) provided on the rear surface of the control box part (40), the main power is cut off, . ≪ / RTI >

When the internal temperature of the culture chamber 11 is lower than the proper temperature due to the temperature stabilizer 43 provided on the rear surface of the control box unit 40, the coil heater unit 17 and the pipe heater unit 16 The user can adjust the internal temperature by turning on or off the power to the touch screen panel 41 and set the appropriate temperature to the touch screen panel 41.

In addition, a temperature recorder 44 for recording the internal temperature is provided to output the internal temperature so that the user can grasp the temperature change with time.

The internal temperature of the culture chamber 11 can be measured by a plurality of temperature sensors 47 provided in the culture chamber 11 and connected to the temperature recorder 44 and the temperature stabilizer 43 The temperature sensor 47 can be easily moved due to the fixed temperature cable 47a fixed to the temperature sensor 47 so that the temperature of the desired position of the culture room 11 can be accurately measured do.

Further, by providing an attachment means (not shown) such as a hook or a hook or the like on one surface of the temperature sensor 47, it can be easily fixed after being moved to a desired position.

The gas inlet 45 is formed at the rear of the control box 40 and the regulator 46 connected to the inside of the gas inlet 45 adjusts the amount of gas supplied from the outside do.

The concentration of the gas distributed in the culture chamber 11 can be measured by the first gas sensor 48a provided in the culture chamber 11 and the second gas sensor 48b And the control signal for the operation of the regulator (46) is outputted. When the gas distributed in the culture chamber (11) is less than or more than the proper concentration, The concentration of the internal gas in the culture chamber 11 can be adjusted by sending a control signal to turn on and off the operation of the regulator 46. The reference of the optimum gas concentration in the culture chamber 11 can be controlled by the touch screen panel 41 It can be set.

In the meantime, gas is moved to a desired position in the culture chamber 11 due to the pressure-resistant gas supply pipe 11a connected to one end of the regulator 46 in the side surface of the culture chamber 11, So that the gas moved by the supply port 11b formed at the end of the pipe 11a can be supplied to the culture chamber 11. [

Here, the gas supplied to the inside of the culture chamber 11 due to the return port 11c connected to the return supply pipe (not shown) connected to the other end of the regulator 46 is circulated inside the culture chamber 11 .

On the other hand, a sampling port 49 connected to the culture chamber 11 is provided on the rear surface of the control box unit 40 and an external meter reading device It is possible to measure the gas concentration inside the first gas sensor 48a and the second gas sensor 48b when the gas sensor 48a and the second gas sensor 48b fail.

The capsule type gas filter portion 11d provided in the gas supply pipe 11a or a part of the sampling port 49 can easily filter impurities distributed in the gas flowing from the outside.

A cartridges 12 having a trapezoidal shape are formed in a lower portion of the culture chamber 11 so that the culture plate cart means 30 is moved by a predetermined movement path, So that it can be easily inserted or ejected.

The rail wheel portion 32 of the culture plate cart means 30 includes a wheel shaft 32a that is inclined outward to be seated on the cart portion 12, Wheel 32b to prevent the culture plate cart means 30 from being disengaged and to be able to work simultaneously with rotation of the wheel 32b.

The wheel slope 32c is formed on the outer circumferential surface of the wheel 32b so that the slant surface of the trapezoidal cartwheel 12 is seated on the sloped surface of the cartrail 12, It is possible to prevent deformation of the wheel axle 32a and significantly improve the replacement timing of the culture plate cart means 30. [

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the appended claims and their equivalents. Of course, such modifications are within the scope of the claims.

1: Cell culture incubator 10:
11: culture chamber 11a: gas supply pipe
11b: supply port 11c: gas filter portion
11d: gas filter portion 12: cart portion
13: outer cover 14: inner cover
15: heat flow path 16: pipe heater part
17: coil heater part 18: heat flow fan
19: Fan cover 19a: Heat sink
19b: filter part 20: door part
30: culture plate cart means 31: culture plate insertion portion
31a: insertion support bar 32: rail wheel part
32a: wheel axle 32b: wheel
32c: wheel slope part 40: control box part
41: Touch screen panel 42: Earth leakage breaker
43: Temperature stabilizer 44: Temperature recorder
45: gas inlet 46: regulator
47: Temperature sensor 47a: Temperature cable
48: gas sensor 48a: first gas sensor
48b: second gas sensor 49: sampling port

Claims (9)

A main body 10 having a plurality of culture chambers 11 in which a cell culture plate P is accommodated, a plurality of door portions 20 provided so as to open and close the front surface of the main body 10, And a control box portion (40) formed on one side surface of the cell culture incubator (10)
A culture plate cart means 30 for inserting or discharging the cell culture plate P is formed inside the culture chamber 11;
A touch screen panel 41 for controlling the operation of the cell culture incubator 1 is provided on the front surface of the control box unit 40. An electric leakage preventing circuit breaker 42 for preventing electric leakage is provided on the rear surface, And a regulator 46 which is connected to the inside of the gas inlet 45 and regulates the amount of the gas to be introduced into the gas inlet 45. The temperature regulator 44, ;
A cartridges 12 of a trapezoidal shape are formed in a lower portion of the culture chamber 11 so that the culture plate cart means 30 is moved by a predetermined movement path;
A plurality of temperature sensors 47 connected to the temperature recorder 44 and connected to the temperature cable 47a, the inner temperature of which is fixed and is not fixed so as to be moved to a predetermined position, is provided in the culture chamber 11;
A first gas sensor 48a for measuring the concentration of the internal gas in the culture chamber 11 and a second gas sensor 48b for measuring the concentration of the internal gas in the culture chamber 11 and controlling the operation of the regulator 46, And a gas sensor (48) comprising a second gas sensor (48b) for outputting gas. The method according to claim 1,
An inner cover 14 formed to be spaced apart from the outer cover 13 by a predetermined distance; and an inner cover 14 formed to be spaced apart from the outer cover 13 by a predetermined distance, And a heat flow path (15) in which a space is formed to allow heat to flow between the outer cover (14) and the outer cover (13). 3. The method of claim 2,
A plurality of coil heaters 17 for generating heat are formed on the side and upper inner side surfaces of the inner cover 14, And a heat flow fan 18 connected to the fan motor M for circulating heat generated from the coil heater unit 17 and the pipe heater unit 16 is provided on the rear surface of the inner cover 14 Wherein the cell culture incubator is equipped with a gas inflow control means. The method of claim 3,
A fan cover 19 is formed on the rear surface of the inner cover 14 so as to cover the front surface of the heat flow fan 18 by a plurality of bolts B, A heat absorbing passage 19a is formed and the heat absorbing passage 19a is provided with a filter 19b for filtering foreign substances distributed in the heat and the gas flowing into the heat absorbing passage 19a. Cell culture incubator. The method according to claim 1,
The culture plate cart means 30 includes a culture plate insertion portion 31 composed of a plurality of insertion support rods 31a into which the cell culture plate P is inserted so that the cell culture plate P is mounted in multiple layers, And a plurality of rail wheels (32) for moving the culture plate cart means (30) to a lower portion of the culture plate insertion portion (31). 6. The method of claim 5,
The rail wheel portion 32 includes a wheel shaft 32a which is outwardly inclined to be seated on the cart portion 12 of the trapezoidal shape and a wheel 32b which is engaged with the wheel shaft 32a and rotates ;
And a wheel inclination part (32c) is formed on an outer circumferential surface of the wheel (32b) so as to be seated on an inclined surface of the trapezoidal cartwheel (12). The method according to claim 1,
A gas supply pipe 11a connected to one end of the regulator 46 for supplying a gas to the culture chamber 11 is formed in a side surface of the culture chamber 11 and an end of the gas supply pipe 11a A supply port 11b for supplying a gas to the culture chamber 11 is formed and a return port 11c for recovering the supplied gas is formed in the culture chamber 11. [ Cell culture incubator. The method according to claim 1,
A sampling port 49 connected to the culture chamber 11 is provided on the rear surface of the control box unit 40 so as to be connected to an external meter reading device for arbitrarily supplying other types of gas from the outside or measuring the concentration of the external gas Wherein the cell culture incubator is equipped with a gas inflow control means. 9. The method according to any one of claims 7 to 8,
And a capsule type gas filter portion (11d) is formed in a part of the gas supply pipe (11a) or the sampling port (49) so that impurities distributed in the gas are filtered.

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