KR20140034891A - Standard marker for determining place of origin or age of processed ginseng, establishing method thereof, or method for determining place of origin or age using the same - Google Patents

Abstract

The present invention relates to a standard marker for determining a place of origin or an age of a processed ginseng, an establishing method thereof, or a method for determining the place of origin or the age using the same. According to the present invention, the standard marker for domestically processed ginseng, that is, white ginseng and red ginseng, is established. Using the standard marker enables an efficient comparative analysis of the place of origin or the age of processed ginseng such as in a concentrate or a powder form, the place of origin or the age of which are not easily distinguishable; thus, the marker can be usefully applied to determine the place of origin or the age of processed ginseng during import-export.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for determining the origin or age of processed ginseng, the same}

The present invention relates to a standard marker for discriminating the origin or age of processed ginseng, a method for establishing the same, or a method for determining the country of origin or age using the same.

In recent years, non-destructive techniques such as near-infrared spectroscopy, electronic nose, and X-ray fluorescence spectroscopy have been widely used. In particular, nondestructive techniques using near-infrared spectroscopy are used to determine the sugar content of fruit vegetables, the moisture content of cereals, Protein and solids content of milk and dairy products and it is possible to utilize existing protein analysis methods such as electrophoresis and HPLC method in order to discriminate the kind or origin of agricultural products, And there was difficulty in application in the field. However, recently, the analysis time is shorter and the resolution is higher than that of the conventional method, so that the time and sample amount required for the analysis can be minimized, and CE chromatogram has been applied as a new technique capable of quantitative digital analysis. However, It is mainly used for discrimination of cultivars (Non-Patent Document 1).

Therefore, it is necessary to effectively distinguish the differences in quality caused by the production and cultivation conditions of various agricultural products including processed ginseng and non-processed ginseng, and to develop a scientific discrimination method to respond to the strengthening and institutionalization of agricultural product indication by the WTO system Since the number of imported agricultural products that are urgently demanded and circulated illegally is increasing day by day, it is required to develop a scientific identification method to check the origin of these agricultural products collected in the field control process.

Panax ginseng C.A. Meyer has been used for thousands of years as a medicinal plant, and ginseng has evolved for a long time in its own environment. Ginseng has been adapted to the environment to be able to live well in its own environment, and ginseng is divided into ginseng, white ginseng, red ginseng according to the processing method, White ginseng is called "dried white ginseng" after it is peeled off and dried in sunlight or hot air. In addition, red ginseng is called "red ginseng" when steamed white ginseng is unpeeled and dried.

Ginseng has long been known to be the best Korean ginseng from all over the world. In recent years, Chinese ginseng has entered the domestic market as a ginseng market, making it difficult to locate domestic ginseng (Non-Patent Document 2). In recent years, In addition, in case of overseas ginseng, ginseng is 3-4 years old so it does not have much significance in the determination of lotus root. However, according to the customary belief that the longer the old age is, the more expensive the year, In the past decade, the distribution system has been disturbed and the reliability of consumers has been reduced. Therefore, the identification of ginseng root is a big problem.

At present, the criteria for the inspection of the root of the agriculture and forestry bureaucrats are to be inspected at the time of harvest and the lotus root is judged. Detailed criteria are the head, body and epidermal shape, degree of leg development, And it is difficult to apply it to the distribution market where the cultivation history is unclear because it is used as an auxiliary discrimination method in the field where the cultivation history is confirmed, . In this study, we have investigated the effect of lysozyme on the lyophilisation of lysozyme. The lyophilisation method has been reported in the past. However, the secretory ductal tissue staining method is evaluated by the accurate discrimination method, and the other methods are low in reliability, and are used as an auxiliary method (see Non-Patent Document 7) and ginsenoside content (non-patent document 8) . However, the secretory ductal tissue staining method is limited to the cultivated ginseng powder because it is identified as the root cross section of the main root, and it is not applicable to the ginseng powder.

Accordingly, the inventors of the present invention, in studying the discrimination method of locally produced or ginseng roots of domestic processed ginseng and Chinese ginseng, separated and analyzed standard markers from domestic processed ginseng and Chinese processed ginseng to discriminate the origin Or a lotus root can be discriminated, and the present invention has been completed.

Kim, Nam-Soo, "Development of identification technology and safety evaluation technology for domestic and imported agricultural products (processed foods)" Seo Dong Sang, "A Study on the Establishment and Effectiveness of Classification System of Korean Cinnamon Ginseng and Chinese Ginseng Ginseng", 2007 Research Report, 2007; J. Ginseng Res. vol. 31, 142-146, 2007; J. Ginseng Res. vol 30, 153-157, 2006; Ginseng Research Report - Cultivation, Korea Ginseng and Tobacco Research Institute p331, 1987; Ginseng Research Report - Cultivation, Korean Ginseng and Tobacco Research Institute p175, 1988; J. Ginseng Res. vol 25, 101-105, 2001; Can. J. Plant Sci. vol. 76, p 855 1996.

An object of the present invention is to provide a marker for discriminating the white ginseng from the white ginseng used for discriminating the origin of white ginseng.

Another object of the present invention is to provide a method for discriminating the origin of white ginseng.

Another object of the present invention is to provide a marker for distinguishing red ginseng from red ginseng, which is used in the identification of the red ginseng.

Another object of the present invention is to provide a method for discriminating the origin of red ginseng.

Another object of the present invention is to provide a marker for determining the age of red ginseng used in the age determination of red ginseng.

Another object of the present invention is to provide a method for determining the age of red ginseng.

In order to achieve the above object, the present invention provides a marker for discriminating the origin of white ginseng represented by the following formula (1) or (2), which is used for discriminating the origin of domestic white ginseng and Chinese white ginseng:

[Formula 1]

(2)

In addition, the present invention provides a method for extracting a metabolite from a white ginseng (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

Comparing the result of the LC-MS analysis obtained in the step 2 with the marker for discriminating the mountain area of the formula (1) or (2) (step 3).

Furthermore, the present invention provides a marker for discriminating the origin of red ginseng represented by the following formula (3) or (4) used for discriminating the origin of domestic red ginseng and Chinese red ginseng:

(3)

[Chemical Formula 4]

The present invention also relates to a method of extracting a metabolite from a red ginseng (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

Comparing the result of the LC-MS analysis obtained in the step 2 with the marker of the mountain area discrimination of the formula (3) or (4) (step 3).

Further, the present invention provides a marker for determining the age of red ginseng represented by the following formulas (5) to (11), which is used for distinguishing the lotus root of Korean 4-year old red ginseng and 6-year old ginseng root ginseng:

[Chemical Formula 5]

[Chemical Formula 6]

[Formula 7]

[Formula 8]

[Chemical Formula 9]

[Formula 10]

[Formula 11]

The present invention also relates to a method of extracting a metabolite from a red ginseng (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

(Step 3) of comparing the LC-MS analysis result obtained in the step 2 with an age discriminating marker selected from the group consisting of the compounds represented by the above-mentioned formulas 5 to 11, to provide an age discrimination method for red ginseng.

According to the present invention, it is possible to establish standard markers in domestic processed ginseng, that is, white ginseng and red ginseng, and to quickly compare and analyze the origin or age of processed ginseng such as concentrate and powder, Therefore, it can be useful for determining the origin or age of processed ginseng at the time of import and export.

FIG. 1 is a view showing an ion chromatogram of a domestic white ginseng extract according to an embodiment of the present invention.
FIG. 2 is a view showing ion chromatogram of Chinese white ginseng extract of an example of the present invention. FIG.
FIG. 3 is a view showing a scorecard plot of domestic white ginseng extract and Chinese white ginseng extract according to an embodiment of the present invention.
FIG. 4 is a graph showing S flots of least square analysis of domestic white ginseng extract and Chinese white ginseng extract according to an embodiment of the present invention. FIG.
FIG. 5 is a graph showing the (a) domestic white ginseng extract and (b) the Chinese white ginseng extract box fillet according to the embodiment of the present invention. FIG.
FIG. 6 is a view showing a mass-trapping system of domestic white ginseng extract of the example.
Fig. 7 is a view showing the mass-trapping method of Chinese white ginseng extract of the Example. Fig.
FIG. 8 is a graph showing ion chromatograms of Korean red ginseng extract according to an embodiment of the present invention.
9 is a view showing ion chromatogram of Chinese red ginseng extract of the example of the present invention.
10 is a view showing a scorecard plot of domestic red ginseng extract and Chinese red ginseng extract according to an embodiment of the present invention.
11 is a diagram showing S flots of the least square analysis of Korean red ginseng extract and Chinese red ginseng extract of the present invention.
FIG. 12 is a view showing the (a) domestic red ginseng extract and (b) the Chinese red ginseng extract box-fillet according to the embodiment of the present invention.
FIG. 13 is a view showing the mass-trapping method of the domestic red ginseng extract of the Example.
Fig. 14 is a view showing the mass-trapping method of Chinese red ginseng extract of the Example. Fig.
FIG. 15 is a graph showing ion chromatograms of 4-year-old Korean red ginseng extract of the present invention.
FIG. 16 is a graph showing ion chromatograms of 6-year old Korean red ginseng extract of the present invention.
17 is a view showing a scorecard plot of 4-year old Korean red ginseng extract and 6-year old red ginseng extract of the present invention.
FIG. 18 is a diagram showing a S-plot of a minimum orthogonal square analysis of 4-year old Korean red ginseng extract and 6-year old green ginseng extract of the present invention.
FIG. 19 is a view showing a box plat of 4-year old Korean red ginseng extract of the present invention.
FIG. 20 is a view showing a box plat of Korean domestic red ginseng extract of the present invention of the present invention. FIG.

Hereinafter, the present invention will be described in detail.

The present invention provides a marker for discriminating the origin of white ginseng represented by the following formula (1) or (2), which is used for discriminating the origin of domestic white ginseng and Chinese white ginseng:

The compound represented by Formula 1 is a marker for discriminating the origin of domestic white ginseng and the compound represented by Formula 2 is a marker for discriminating the origin of Chinese white ginseng.

The markers for determining the origin of white ginseng represented by Chemical Formulas 1 or 2 according to the present invention include extracting metabolites from domestic white ginseng and Chinese white ginseng (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

In step 2, the results analyzed by the LC-MS are converted into numerical values that can be processed statistically. From the data obtained by analyzing the converted numerical values by the multivariate analysis method, components showing differences between the groups are detected, And establishing it as a standard marker along the origin (step 3).

Specifically, step 1 according to the present invention is a step of extracting metabolites from domestic white ginseng and Chinese white ginseng, and it is preferable to use water, alcohol or a mixture thereof as the extraction solvent. The alcohol is preferably a C ₁ to C ₄ lower alcohol, more preferably ethanol or methanol, most preferably 70% methanol, but is not limited thereto. Examples of the extraction method include filtration, hot water extraction, immersion extraction, reflux cooling extraction, ultrasonic extraction, and the like, and the extraction is preferably performed once to five times by the ultrasonic extraction method, But is not limited thereto. The extraction temperature is preferably 20 to 30 占 폚, but is not limited thereto.

The step 2 is a step of analyzing the metabolite extracted in the step 1 with a liquid chromatography-mass spectrometer (LC-MS), and column chromatography was carried out at 30-40 ° C, Water and formic acid (100: 0.1, v / v) and mobile phase B was used as acetonitrile and formic acid (100: 0.1, v / v). The flow rate of the mobile phase is 0.4 ml / min and the sample injection amount can be measured by taking 1 μl of the domestic white ginseng extract and the Chinese white ginseng extract sample, respectively. In addition, the mass spectrometer can use, but is not limited to, a quadrupole orthogonal acceleration flight time tandem mass spectrometer.

Further, in step 3, the result analyzed by LC-MS is converted into a statistically processable value, and the converted numerical value is analyzed by multivariate analysis to detect components showing differences between data and groups. Then, And the standard markers of domestic white ginseng and white ginseng are established as standard markers according to the mountain area. The multivariate analysis method used at this time is orthogonal least-squares analysis, and the data obtained by analyzing with the multivariate analysis method are used as a score scatter plot You can see the standard markers.

In addition,

Extracting a metabolite from the white ginseng to be tested (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

Comparing the result of the LC-MS analysis obtained in the step 2 with the marker for discriminating the mountain area of the formula (1) or (2) (step 3).

The method for determining the origin of white ginseng can be used for discriminating the origin of white ginseng at the time of import and export because it is possible to quickly compare white ginseng seeds such as concentrate and powder which are difficult to identify the origin, using standard markers.

Furthermore, the present invention provides a marker for discriminating the origin of red ginseng represented by the following formula (3) or (4) used for discriminating the origin of domestic red ginseng and Chinese red ginseng:

Here, the compound represented by Formula 3 is a marker for discriminating the origin of domestic red ginseng, and the compound represented by Formula 4 is a marker for discriminating the origin of Chinese red ginseng.

Further, the markers for discriminating the origin of red ginseng represented by the above formula (3) or (4) according to the present invention may include a step (step 1) of extracting metabolites from Korean red ginseng and Chinese red ginseng;

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

In step 2, the results analyzed by the LC-MS are converted into numerical values that can be processed statistically. From the data obtained by analyzing the converted numerical values by the multivariate analysis method, components showing differences between the groups are detected, And establishing it as a standard marker along the origin (step 3).

Specifically, the steps 1 to 3 may be performed in the same manner as in the method for determining the mountain area of white ginseng, but the present invention is not limited thereto.

In addition,

Extracting a metabolite from the red ginseng (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

Comparing the result of the LC-MS analysis obtained in the step 2 with the marker of the mountain area discrimination of the formula (3) or (4) (step 3).

The method of determining the origin of red ginseng can be used for discriminating the origin of red ginseng at the time of import and export because it is possible to quickly compare the origin of red ginseng such as concentrate and powder, which are difficult to identify the origin, using standard markers.

Further, the present invention provides a marker for determining the age of red ginseng represented by the following formulas (5) to (11), which is used for distinguishing the lotus root of Korean 4-year old red ginseng and 6-year old ginseng root ginseng:

The compounds represented by Formulas 5 to 8 are markers for age determination of 4-year old red ginseng, and the compounds represented by Formulas 9 to 11 are markers for age determination of 6-year old red ginseng.

The marker for determining the age of red ginseng represented by the above Chemical Formulas 5 to 11 according to the present invention comprises the steps of: (1) extracting metabolites from domestic 4-year old red ginseng and 6-year old ginseng red ginseng;

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

In step 2, the results analyzed by the LC-MS are converted into numerical values that can be processed statistically. From the data obtained by analyzing the converted numerical values by the multivariate analysis method, components showing differences between the groups are detected, And establishing it as a standard marker according to the age of red ginseng (step 3).

Specifically, the steps 1 to 3 may be performed in the same manner as in the method for determining the mountain area of white ginseng, but the present invention is not limited thereto.

In addition,

Extracting a metabolite from the red ginseng (step 1);

Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And

(Step 3) of comparing the LC-MS analysis result obtained in the step 2 with an age discriminating marker selected from the group consisting of the compounds represented by the above-mentioned formulas 5 to 11, to provide an age discrimination method for red ginseng.

The age determination method of the red ginseng can be used to determine the age of red ginseng at the time of import and export because it is possible to quickly compare the age of red ginseng such as concentrate and powder which is difficult to identify with age using a standard marker.

Hereinafter, the present invention will be described in detail by the following examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

Preparation Example 1 Preparation of Processed Ginseng

53 domestic white ginsengs were purchased from Korea for the discrimination of Korean and Chinese white ginsengs regardless of lotus root (age), 18 white ginsengs were purchased from China and domestically, and in order to discriminate the origin of Korean and Chinese red ginseng 50 Korean red ginseng were purchased in Korea and 35 Chinese red gins were purchased in China and in Korea. In addition, red ginseng was used to distinguish four root and six root ginseng from red ginseng by using 4 root and 10 root and 6 root and 10 root ginseng from Icheon, Gyeonggi - do.

Example 1 Establishment of a Standard Marker for Identifying the Origin of White Ginseng

The following experiment was carried out to establish markers for discrimination between the domestic white ginseng and the Chinese white ginseng.

Step 1: Extraction of white ginseng metabolite

Each of the domestic white ginseng and white ginseng prepared in Preparation Example 1 was dried and then pulverized to obtain 0.2 mg of each. Then, 5 ml of 70% methanol was added and the resultant was irradiated for 1 hour with a 350 W sonicator. After filtration through a membrane filter (SUN-SRi, TN, USA), the extract was collected.

Step 2: Analysis of white ginseng extract by liquid chromatography-mass spectrometry (LC-MS)

In order to analyze the white ginseng extract metabolites, prepared as described in Step 1 UPLC was used Acuity UPLC system Waters, column Acuity ™ BEHC ₁₈ in _{(ACQUITY UPLC ™ BEHC 18) (} 10 mm × 2.1 mm, id (100: 0.1, v / v) and mobile phase B were acetonitrile and formic acid (100: 0.1, v / v) The mobile phase B was initially increased from 10% to 30% to 7 minutes, then increased to 44% to 14 minutes and to 100% to 21 minutes. The mobile phase B was then diluted to 100% , And the mobile phase B was lowered to 10% at 23.6 min and then equilibrated and used for the next analysis. The flow rate of the mobile phase was 0.4 ml / min and the sample injection amount was 1 μl each of the domestic white ginseng extract and the Chinese white ginseng extract sample.

The Q-TOF mass spectrometer was a quadrupole orthogonal acceleration time-of-flight tandem mass spectrometer (Waters Q-TOF Premier ™) from Waters. The analysis conditions of the mass spectrometer are shown in Table 1 below.

Ion source ESI + Nebulizer gas 500 L / h Nebulizer Temperature 350 ℃ Con gas 50 L / h Source temperature 100 ℃ Capillary voltage 2.7 kV Con Voltage 27 V

The results of the total ion chromatograms of the liquid white-ginseng extract and the white ginseng extract of the domestic white and ginseng extracts (LC-MS) are shown in FIGS. 1 and 2.

Step 3: Transform to a statistical value and establish white marker standard markers

Peak finding, peak alignment, and the like are performed using Waters' MarkerLynx application manager (ver. 4.1) to convert the LC-MS data obtained in step 2 into statistically processed values. And peak filtering were performed. The analytical data range is 200-1500 Da, the mass error tolerance range is 0.02 Da, and the mass noise level is the base peak intensity. . In all the LC-MS data obtained from this procedure, the retention time (RT) value and the mass value for each peak were matched and the area value of each peak was obtained. Then, for the multivariate statistical analysis, Simca-P + ver. (OPLS-DA) scores by grouping domestic and Chinese white ginsengs with par- scaled (scaled to square root of SD) using 12.0 (Umetrics, Sweden) (Simca-P +) of Orthogonal Least Squares analysis, and the natural peaks that can discriminate by boxplotting the components having a large difference between two mountain regions are selected and their mass ion chromatogram [mass ion chromatogram, MassLynx (ver 4.1)], and the standard marker was selected as the mass fragmentation pattern. The results are shown in Figs.

result

(1) As a result of the score scatter plot of the orthogonal least-squares analysis, as shown in Fig. 3, domestic white ginseng and Chinese white ginseng were divided into two groups according to their mountain regions.

(2) Further, as a result of the S-PLOT of the orthogonal least-squares analysis, as shown in Figs. 4 and 5, there is a significant difference between components having a mass of 963.5582 of 5.21 and components having a mass of 771.4919 of 9.30 (T-test; p> 0.001).

(3) Furthermore, as shown in FIGS. 6 and 7, the standard markers of domestic white ginseng contained 20-glucosinenoside Rf (5.21 min, MW 963.5582), and the standard marker of Chinese white ginseng was identified as notozynecoside R2 (9.30 min, MW 771.4919) as shown in the following formula (2).

      [Chemical Formula 1] < EMI ID =

≪ Example 2 > Establishment of standard markers for discrimination of red ginseng

The following experiment was carried out to establish markers for discrimination between the domestic red ginseng and the Chinese red ginseng.

Step 1: Extraction of red ginseng metabolite

The procedure of Step 1 of Example 1 was repeated except that the Korean red ginseng and the Chinese red ginseng prepared in Preparation Example 1 were used.

Step 2: Analysis of red ginseng extract using liquid chromatography-mass spectrometer (LC-MS)

The procedure of Step 2 of Example 1 was repeated except that the metabolite of the red ginseng extract prepared in Step 1 was used.

The total ion chromatogram results of the liquid chromatographic-mass spectrometry (LC-MS) of the Korean red ginseng extract and the Chinese red ginseng extract are shown in FIGS. 8 and 9.

Step 3: Conversion into statistical processable values and establishment of red ginseng standard markers

The procedure of Step 3 of Example 1 was repeated except that the result obtained in Step 2 was used.

result

(1) As a result of the score scatter plot of the orthogonal least squares analysis, as shown in FIG. 10, domestic red ginseng and Chinese red ginseng were divided into two groups according to the mountain area.

(2) Further, as a result of the S-PLOT of the orthogonal least-squares analysis, as shown in Figs. 11 and 12, there is a significant difference between the component having a mass of 801.4988 of 9.04 and the component having a mass of 767.4952 of 11.70 (T-test; p> 0.001).

(3) Furthermore, as shown in FIGS. 13 and 14, the standard marker of domestic red ginseng was a ginsenoside Rf (9.04 min, MW 801.4988) as shown in the following formula (3) , And the standard marker of Chinese red ginseng was identified as ginsenoside Rd (11.70 min, MW 767.4952) as shown in the following formula (4).

      [Chemical Formula 3]

<Example 6> Establishment of standard markers for discrimination of age (4 years old and 6 years old) of red ginseng

The following experiment was conducted to establish markers for the identification of the four - year old Korean red ginseng and six - year old ginseng Korean red ginseng.

Step 1: Extraction of red ginseng metabolite

The same procedure as in Step 1 of Example 1 was conducted except that 4-year-old domestic red ginseng prepared in Preparation Example 1 and 6-year old domestic ginseng red gins were used.

Step 2: Analysis of red ginseng extract using liquid chromatography-mass spectrometer (LC-MS)

The procedure of Step 2 of Example 1 was repeated except that the metabolite of the red ginseng extract prepared in Step 1 was used.

The total ion chromatogram results of liquid chromatographic-mass spectrometry (LC-MS) of the Korean 4-year old red ginseng extract and 6-year old ginseng extract are shown in FIGS.

Step 3: Conversion into statistical processable values and establishment of red ginseng standard markers

The procedure of Step 3 of Example 1 was repeated except that the result obtained in Step 2 was used.

result

(1) As a result of the score scatter plot of the orthogonal least-squares analysis, as shown in FIG. 17, domestic 4-year old red ginseng and 6-year old red ginseng were divided into two groups according to lotus root.

(2) Also, as shown in Figs. 18 to 20, the S-plot results of the orthogonal least-squares analysis showed that there was a significant difference in the masses of the 4-year old red ginseng and the 6-year old ginseng red ginseng (t-test; p> 0.001).

Among them, malonyl ginsenoside Rb1 (1195.6145 of 9.89) (Formula 5), malonyl ginsenoside Rc (10.30 min, MW 1165.6030) 6, malonyl ginsenoside Rb2 Min, MW 1165.6036), malonyl ginsenoside Rd (12.02 min, MW 853.4980), and the 6-year old red ginseng component was identified as (R) -ginnenoside Rh1 1277.8934), queenquenoside R1 (11.26 min, MW 1151.6239) and (R) -ginnenoside Rg3 (15.80 min, MW 749.4871).

The 4-year-old red ginseng components and the 6-year-old red ginseng components are shown in Table 2 below.

The rescue Name of compound




5

Malonyl ginsenoside Rb1




6

Malonyl ginsenoside Rc




7

Malonyl ginsenoside Rb2




8

Malonyl ginsenoside Rd



9

(R) -ginsenoside Rh1




10

Queen Quenoside R1



11

(R) -ginsenoside Rg3

Claims (7)

Marker for determining the origin of domestic red ginseng and Chinese red ginseng of the compound represented by the formula (3) or (4).
(3)

[Chemical Formula 4]

According to claim 1, wherein the compound represented by the formula (3) is a marker for determining the origin of domestic red ginseng and Chinese red ginseng, characterized in that the marker for determining the origin of domestic red ginseng.
According to claim 1, wherein the compound represented by the formula (4) is a marker for determining the origin of domestic red ginseng and Chinese red ginseng, characterized in that the marker for determining the origin of Chinese red ginseng.
Extracting metabolites from domestic red ginseng and Chinese red ginseng (step 1);
Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And
In step 2, the results analyzed by the LC-MS are converted into numerical values that can be processed statistically. From the data obtained by analyzing the converted numerical values by the multivariate analysis method, components showing differences between the groups are detected, A method for establishing a marker for identifying a red ginseng mountain area according to claim 1, comprising a step (step 3) of establishing as a standard marker along the mountain area.
5. The method according to claim 4, wherein the multivariate analysis method of step 3 is an orthogonal least squares analysis method.
5. The method according to claim 4, wherein the data obtained by analyzing the multivariate analysis of step 3 is a scorecard plot.
Extracting a metabolite from the red ginseng (step 1);
Analyzing the metabolites extracted in step 1 with a liquid chromatography-mass spectrometer (LC-MS) (step 2); And
Comparing the result of the LC-MS analysis obtained in the step 2 with the marker of the mountainous region of the formula (3) or (4) of claim 1 (step 3).

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