KR20140018679A - Portable cartridge for analyzing ingredient of sample - Google Patents

Abstract

The present invention relates to a portable cartridge for analyzing the ingredient of a sample wherein the portable cartridge forms multiple body parts, where different kinds of reagents are stored, in the shape of a container, shields upper and lower parts with a cover of film shape, multiply combines the body parts where required reagents are stored for simply mixing the sample and reagents. The portable cartridge includes a measuring body formed in the shape of the container for the sample to be stored through a portable analyzing device to store the reagents at inside for analyzing the sample in advance, having the cover for shielding formed at least one side of the upper and lower parts, combined with one or more bodies and the lower part of the body, formed in the shape of the container for closing lower surface and opening upper surface, storing the reagents at inside, having the cover at the opened upper surface.

Description

PORTABLE CARTRIDGE FOR ANALYZING INGREDIENT OF SAMPLE

The present invention relates to a portable cartridge for sample component analysis, and more particularly, to form a plurality of bodies each of which stores different types of reagents in a tubular shape, and to seal the upper and lower parts with a cover of a membrane, to store the necessary reagents The present invention relates to a portable cartridge for sample component analysis, which combines bodies in multiple stages to simply mix a sample and a reagent.

In general, there is an increasing demand for on-site diagnosis, in which the concentration of components to be detected is measured directly at the site of use and immediately reflects the results.

Usually these sites of use are those where monitoring or immediate prescription is necessary because problems arise when the concentration of certain components exceeds a certain range.

For example, in order to recover from abnormal growth of crops, it is necessary to measure the concentrations of the components present in the soil and take necessary measures, or to measure the causes of pollution by measuring the concentrations of various components in the streams where the pollution occurred. On-site diagnostics are needed to remove the source.

Conventional analytical methods for analyzing various components include instrumental analysis, analytical methods using analytical kits, analytical methods using ion-selective permeable membrane electrodes, and the like.

First, instrumental analysis is performed using expensive analytical equipment. Although the accuracy is very high, the portability of the analytical equipment is low enough to make field diagnosis and complex pretreatment process is required. Not only is it necessary, but it also requires a lot of analysis time, so it is not suitable as an on-site diagnostic method.

Second, analysis kits for measuring the concentration of specific components currently on the market are composed of component analysis reagents provided by manufacturers and portable measuring instruments (eg, portable spectrophotometers) or color tables.

Although the assay kit itself has a certain degree of portability, in order to use the assay reagent provided by the manufacturer, it is necessary to use a variety of additional analytical instruments including a pipette, thereby reducing practical portability.

In addition, it is difficult or inconvenient to implement the exact amount of reagents suggested by the manufacturer in the field. Especially for non-experts, the accuracy of the analysis is inferior.

Third, the recently developed ion concentration measuring method using an ion selective permeable membrane electrode is very convenient because it measures the concentration of a specific ion only by contacting a sample to be analyzed with a specific electrode, which is suitable for on-site diagnosis.

However, this method has its limitation because there are components that cannot be measured because there is no ion-selective permeable membrane electrode developed to date. In particular, there is a problem that in-situ diagnosis is impossible because an electrode for phosphoric acid, which is one of the important components in water quality analysis or soil analysis, which requires on-site diagnosis from a comprehensive analysis result value of a plurality of components is not developed. In addition, in the case of the ion-selective permeable membrane electrode, the use concentration range is very narrow and the analysis is often hampered by low concentration of interfering ions, and thus there are many limitations in actual use.

Most component analysis methods consist of adding the following reagents: Adding reagents that specifically react with the component to be analyzed, such as enzymes or color reagents, adding reagents that match the pH or redox conditions so that this specific reaction occurs, and reagents that remove interfering ions Adding step and measuring spectroscopically or electrochemically. Each reaction step may be omitted or reordered depending on the nature of the component analysis method.

The present inventors can implement the component analysis method suitable for the on-site diagnosis by preparing each reagent required for the reaction in the cartridges based on the outline of the component analysis method.

Related prior art is Korean Patent Publication No. 10-2012-0041240.

The present invention forms a plurality of bodies, each of which stores different types of reagents in a cylindrical shape, seals the upper and lower portions with a membrane-shaped cover, and simply mixes the samples and reagents by combining the bodies in which the necessary reagents are stored in multiple stages. It is to provide a portable cartridge for sample component analysis.

The technical objects to be achieved by the present invention are not limited to the above-mentioned technical problems.

Portable cartridge for sample component analysis of the present invention for achieving the above object is formed in a cylindrical shape so that the sample is stored through a portable analysis mechanism is stored in advance for the reagent for analyzing the sample therein, the top and bottom for sealing The cover is formed on one or more sides, the body is composed of one or more, and coupled to the lower portion of the body, formed in a cylindrical shape, the lower side is closed, the upper side is opened, the reagent is stored therein, the opening It may include a measuring body in which the cover is formed on the upper side.

Specifically, the connection between the body and the body, and between the body and the measuring body, the body is coupled to the upper and lower sides and may further include a connecting portion formed in the center of the damaged body to break the cover formed on the measuring body.

The body stores a reagent therein, and may include a first coupling part formed at an upper end of the cover or the body or the connection part, and a second coupling part formed at the lower end of the body or the connection part.

The body, the measuring body and the connecting portion may be formed of a chemical resistant material.

The measuring body may be connected to the body or the connecting portion is formed by the first coupling portion in the upper opening.

The body, the connecting portion and the measuring body are formed to be coupled to the secondary when not in use to maintain the primary coupling state, when using the secondary coupling to break the cover so that the upper body and the lower body or the reagent of the measuring body to be mixed Can be.

The measuring body is made of a transparent material when measured by the spectroscopic method through the portable analysis device, the electrode may be formed to enable an electrochemical analysis when measured by the electrochemical method.

As described above, according to the present invention, the reagents to be analyzed are quantitatively pre-divided into each of the reaction reagents, each of which is contained in a tubular body. Minimized use has the advantage of portability.

In addition, since the reaction reagents are provided in a quantitatively divided state through the QC (Quality Control) manufacturing process, the analysis error can be remarkably reduced according to the individual analyst, and thus, the analysis reproducibility is good and the analysis time can be shortened. .

In addition, since the analysis method is simple and the use of the analysis equipment is minimized, it may be an analytical method that can be used by non-experts, and the amount of reaction reagents used is small, thereby reducing secondary contamination.

1 is a cross-sectional view showing the overall configuration of a portable cartridge for sample component analysis according to an embodiment of the present invention.
FIG. 2 is an enlarged cross-sectional view of portion 'A' of FIG. 1.
3 is a cross-sectional view showing a separation of the portable cartridge for sample component analysis according to the present invention.
Figure 4 is a view showing the connection between the body and the body and the body and the measurement body of the portable cartridge for sample component analysis according to the present invention.
Figure 5 is an exploded view showing the connection between the body and the body and between the body and the measuring body of the portable cartridge for sample component analysis according to the present invention.
Figure 6 is a plan view showing a connection of the portable cartridge for sample component analysis according to the present invention.
7 is a plan view showing that the crushed body of the connection portion of the portable cartridge for sample component analysis according to the present invention is formed to protrude in the center.
8 is a graph of measuring nitrite nitrogen using a portable cartridge for sample component analysis according to the present invention. (Y is the slope curve on the graph, R ² is the accuracy of the experiment.)

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same components are denoted by the same reference symbols whenever possible. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

1 to 3 is a view showing a portable cartridge for sample component analysis according to an embodiment of the present invention, a cylindrical shape is a reagent is stored therein, the cover 50 is formed on the upper and lower openings Or a plurality of bodies 10, coupled to the lower side of the body 10, a reagent is stored therein, a measuring body 40 having only an upper portion thereof, and inserted between the body 10 and the measuring body 40. It includes a connecting portion 70 for connecting the body 10 and the measuring body 40.

The body 10 is formed in a cylindrical shape, the upper and lower openings are formed, and a storage space for storing reagents is formed therein, and a cover 50 is formed at the upper and lower portions so that the reagents therein do not flow out.

The body 10 is made of a chemical resistant material and may be formed transparent or opaque.

The body 10 has a first coupling portion 20 is formed so that the stopper (1) or the other body 10 to block the opening of the upper portion at the upper portion, the other body 10 or the measuring body 40 at the lower portion ) Is coupled to the second coupling part 30.

The lower side of the body 10 is connected to the measuring body 40 which can measure the sample mixed with the reagent through an external portable analysis instrument (not shown).

The measuring body 40 is formed in a cylindrical shape, the lower part is closed, only the upper part is opened so that the reagent is stored therein, and a cover 50 is formed on the upper part so that the reagent does not flow out.

The measuring body 40 is made of a chemical resistant material and may be formed transparent or opaque.

The mixture of the sample and the reagents finally stored in the measuring body 40 is measured through a portable analysis instrument (not shown), and transparent in the case of spectroscopic measurement, which is a measurement method of a portable analysis instrument (not shown). The measuring body 40 is used, and in the case of a portable analysis device (not shown) using an electrochemical analysis method, an electrode 41 is formed inside the measuring body 40 and an external portable analysis device (not shown) is used. Connected.

The cover 50 formed in the body 10 and the measuring body 40 configured as described above is formed in a thin film form, and is made of a metal or plastic material having chemical resistance, and may be bonded through a high frequency bonding method or bonded using an adhesive. It can also be joined via heat.

The cover 50 formed on the lower portion of the body 10 may be integrally formed when the body 10 is formed.

Reagents are stored in the body 10 and the measuring body 40 configured as described above, and mixed with the sample introduced through the upper portion of the body 10 to facilitate analysis through a portable analysis device (not shown).

The first coupling portion 20 and the second coupling portion 30 formed on the body 10 and the measurement body 40 are formed to be coupled to the secondary, so that the cover 50 is not damaged during the primary coupling. When the sample is put into the body 10, the sample is combined with the secondary, and the cover 50 is broken by the secondary, which causes the sample and the reagent to be stored into the body 10 or the measuring body 40 at the next stage. Allow to mix.

The first coupling portion 20 and the second coupling portion 30 may be formed in the form of a screw can be coupled through rotation, the locking projection and the locking groove is the first coupling portion 20 and the second coupling portion 30 The first coupling part 20 and the second coupling part 30 may be coupled to each other.

As shown in FIGS. 4 to 5, the body 10 and the measurement body 40 may be directly coupled, but may be coupled through the connection part 70.

The connecting part 70 is inserted between the body 10 and the measuring body 40 so that the first coupling part 20 and the second coupling part 30 of the body 10 and the measuring body 40 are inserted and coupled. .

6 to 7, the breaker 60 is formed to break the cover 50 formed on the body 10 and the measuring body 40 at the stop as shown in FIGS. 6 to 7.

The damaged body 60 is supported by the support because the mixture of the sample and the reagent stored in the upper body 10 as shown in FIG. 7 should all flow into the lower body 10 or the measuring body 40. It is formed into a shape.

That is, a plurality of through holes are formed in the main surface of the breaker 60 to allow the mixture stored in the upper body 10 to pass therethrough.

The first coupling part 20 and the second coupling part 30 connecting the body 10 and the measurement body 40 with the connection part 70 may be formed in a screw shape, and the first coupling part 20 ) And the connection part 70 into which the second coupling part 30 is inserted and connected may have a screw contact surface corresponding to the screw shape of the first coupling part 20 and the second coupling part 30. When the coupling protrusion 31 is formed in the first coupling part 20 and the second coupling part 30 or the connection part 70, the corresponding coupling groove 21 is the connection part 70 or the first coupling part 20. ) And the second coupling part 30 may be combined.

When the connection portion 70 and the first coupling portion 20 and the second coupling portion 30 are coupled to each other can be formed to be coupled, for this reason, the cover body 50 is formed with a reagent stored state Before using the 10 and the measuring body 40, the cover 50 should not be damaged so that the cover 50 and the damaged body 60 of the connecting portion 70 are coupled to be spaced apart.

Then, the sample is put into the body 10 to mix the sample and the reagent, and when mixing the reagent and the mixture stored in the body 10 or the measuring body 40 of the next stage body 10 and the connecting portion 70 and The measuring body 40 is secondarily coupled according to the required portion so that the mixture and the reagent of the lower body 10 or the measuring body 40 can be mixed.

Reagents stored in the body 10 or the measuring body 40 may be changed according to the analysis method or the type of the sample of the body 10 and the measuring body 40.

The method of mixing the sample with the reagent through the above configuration is as follows.

The other body 10 is coupled to the lower side of the body 10, the stopper 1 is coupled to the top, and the measurement body 40 is coupled to the far end.

At this time, the number of the body 10 can be changed as necessary, the reagents stored in each of the body 10 consisting of a plurality of each is stored in a different type depending on the analysis.

The body 10 and the measurement body 40 may be directly coupled through the first coupling part 20 and the second coupling part 30, or may be connected through the connection part 70.

The configuration as described above is made of a single module.

When putting the sample in the cartridge configured as described above, first, open the stopper (1) coupled to the uppermost body (10), and put the sample into the body of the uppermost layer (10), cover the stopper (1) and shake when the sample is finished The reagent and the sample stored in the uppermost body 10 are mixed.

Then, the upper body 10 and the body 10 of the suspension are pressurized to be combined, and at the same time, the cover 50 is broken and a mixture of reagents and samples stored in the top body 10 is mixed. 10).

Then, the mixture introduced into the suspension and the reagent stored in the suspension body 10 are shaken to mix.

In this case, one or more body 10 may be used depending on the use environment and the type of sample to be measured.

Thus, when the body 10 is composed of a plurality of reagents stored in each of the body 10 is stored in a different kind of liquid state or powder state according to its characteristics.

When the mixture of the reagent and the mixture inside the body of the suspension 10 is combined with the measuring body 40 coupled to the lower body 10 and the lowermost at the same time, the cover 50 is broken and the mixture of the suspension is measured body ( 40).

When the mixture is introduced into the measuring body 40, the mixture and the reagent are shaken to be mixed, the reagent and the mixture are mixed, and the mixture of the measuring body 40 is analyzed through a portable analyzer (not shown).

In this case, the portable analyzer (not shown) is analyzed using a spectroscopic method or an electrical analysis method.

When measuring the mixture inside the measuring body 40 by a spectroscopic method through the portable analysis device (not shown) to measure the absorbance inside the measuring body 40 using the measuring body 40 made of a transparent material do.

When measuring the mixture inside the measuring body 40 by an electrical analysis method through the portable analysis device (not shown), the measuring body 40 in which the electrode 41 is formed is used.

The electrode 41 formed on the measuring body 40 may be manufactured separately and inserted into the measuring body 40 or may be formed as a circuit pattern on the measuring body 40.

Referring to the experiment of this process as an example as follows.

Example: Nitrite Nitrogen Measurement

1,200ul of acetic acid having a concentration of 0.15% is stored in the uppermost body 10 in which the cover 50 is formed and sealed at the lower portion thereof, and the upper portion is closed with a stopper 1. In addition, 200ul of sulfonylamide, which is a reagent having a concentration of 1%, is stored in the body 10 of the suspension, and a cover 50 is formed in the upper and lower openings to seal it.

In addition, 100ul of N- (1-naphthyl) ethylenediamine dihydrochloride, which is a reagent having a concentration of 0.2%, is stored in the measuring body 40, and the cover 50 is attached and sealed at the top.

100ul of the sample is put into the body 10 of the uppermost layer thus configured and mixed with acetic acid which is a reagent inside the body 10. Then, the body 10 of the middle layer 10 and the body 10 of the uppermost layer are secondarily coupled to break the cover 50, thereby storing the mixture of the sample and the reagents inside the upper body 10 and the body 10 of the middle layer. Mix the reagents. Then, the intermediate body 10 and the measuring body 40 are secondarily combined to mix a mixture of the sample and the reagent stored in the uppermost layer and the middle body 10 of the sample and the reagent inside the measuring body 40.

After 10 minutes of the mixture stored in the measuring body 40, the absorbance of the mixture is measured through an external portable analyzer (not shown). The result is shown in FIG.

Here, the body 10 and the measuring body 40 are configured in a state where the first coupling is to be a secondary coupling, the first coupling is coupled in a state that the cover 50 is not broken.

According to the present invention configured as described above, the use of additional analytical instruments at the site of use is performed by combining the reaction reagents, which are divided by the quantitatively, into the respective bodies contained in the barrel-shaped body. Minimized portability is good.

In addition, since the reaction reagents are provided in a quantitatively divided state through the QC (Quality Control) manufacturing process, the analysis error can be remarkably reduced according to the individual analyst, and thus, the analysis reproducibility is good and the analysis time can be shortened. .

In addition, since the analysis method is simple and the use of the analysis equipment is minimized, it may be an analytical method that can be used by non-experts, and the amount of reaction reagents used is small, thereby reducing secondary contamination.

The portable cartridge for analyzing sample components is not limited to the configuration and operation of the embodiments described above. The above embodiments may be configured such that various modifications may be made by selectively combining all or part of the embodiments.

1: stopper
10: Body
20: first coupling portion 21: coupling groove
30: second coupling portion 31: coupling projection
40: measuring body 41: electrode
50: cover 60: broken body
70: connection

Claims (7)

A body formed in a cylindrical shape so that the sample is stored through the portable analysis device, and a reagent for analyzing the sample is stored in advance, and a sealing cover is formed on one or more sides of the upper and lower parts, and the body comprises one or more; And
The measuring body is coupled to the lower portion of the body, formed in a cylindrical shape, the lower side is closed and the upper side is opened, the reagent is stored therein, and a cover is formed on the opened upper side. Portable cartridge.
The method according to claim 1,
A portable cartridge for analyzing sample components further comprising a connection portion connecting the body and the body and between the body and the measurement body, the body being coupled to an upper side and a lower side, and a damaged body formed to break the cover formed on the measurement body. .
The method according to claim 2,
The body has a reagent stored therein,
A first coupling part formed at an upper end of the cover or the body or the connection part; And
And a second coupling part formed at a lower end of the body or the connection part to be coupled thereto.
The method according to claim 2,
Portable body for sample component analysis of the body, the measuring body and the connecting portion is formed of a chemical resistant material.
The method according to claim 2,
The measuring body is a portable cartridge for sample component analysis, the first coupling portion is formed in the upper opening is connected to the body or the connecting portion.
The method according to claim 2,
The body, the connecting portion and the measuring body are formed to be coupled to the secondary when not in use, the primary coupling state is maintained, the secondary bonding when used to break the cover so that the upper body and the lower body or the reagent of the measuring body is mixed Portable cartridge for sample composition analysis.
The method according to claim 1 or 2,
The measuring body is made of a transparent material when measured by the spectroscopic method through the portable analysis device, the portable cartridge for sample component analysis, the electrode is formed so that the electrochemical analysis when measured by the electrochemical method.

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