CN217587022U - Sample detection device - Google Patents
Sample detection device Download PDFInfo
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- CN217587022U CN217587022U CN202221086360.4U CN202221086360U CN217587022U CN 217587022 U CN217587022 U CN 217587022U CN 202221086360 U CN202221086360 U CN 202221086360U CN 217587022 U CN217587022 U CN 217587022U
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Abstract
The utility model discloses a sample detection device, which relates to the field of medical appliances and comprises a shell, a sample adding chamber, a sample detection channel and a calibration liquid channel, wherein the sample adding chamber, the sample detection channel and the calibration liquid channel are all arranged inside the shell; and a reference electrode which is not covered with a polymer membrane is arranged in the calibration solution channel. The utility model discloses the reference electrode that will not drip the coating polymer membrane establishes and carries out independent reference in the calibration solution passageway, can the independent detection index of independent combination to can the steady electric potential of holding, can not disturb the result that detects out yet.
Description
Technical Field
The utility model relates to the field of medical equipment, especially, relate to a sample detection device.
Background
The current sensors on the market can complete the experiment only by matching a reference electrode and a working electrode together, the purpose of the reference electrode is to keep stable potential, and the reference electrode is usually realized by dripping a polymer film.
The reference electrode is nearly ideally unpolarized, essentially no current passes through it, a good reversible electrode is necessary, and the electrode potential conforms to the nernst equation, and after a very small current flow, the potential of the electrode rapidly returns to its original position, although the stability and reproducibility of the potential must be good. There are three main types of commonly used reference electrodes: a standard hydrogen electrode, a calomel electrode and a silver/silver chloride electrode, wherein the calomel electrode and the silver/silver chloride electrode are most commonly used, and the potentials of the calomel electrode and the silver/silver chloride electrode are related to the concentration of chloride ions. For example, chinese patent CN113340952A provides an instant diagnosis test device and its application, belonging to the technical field of in vitro diagnosis. The device comprises a substrate, a first sealing film, a second sealing film, a sample driving part, a reagent storage part and an array sensor, wherein the reagent storage part is integrated on the substrate in a mode of two channels and independent reference electrodes, the reagent storage part is punctured by a rectangular pyramid with a flow guide groove formed in one side of the substrate to allow a reagent to flow out, a buffer channel in the form of a water storage groove is adopted to filter bubbles in a test sample and a calibration solution, and the device is used for analyzing biological or chemical substances in a blood sample or a metabolite sample by utilizing an electrochemical principle. However, the sensor is independent of the ion selective electrode site and the reference electrode site, and the structure of the sensor is complicated.
In addition, in the array type sensor for detecting chloride ions, the reference electrode often participates in weak reaction, so that a stable potential cannot be maintained, and the calculation of the ion concentration of the whole chloride ions is influenced. Even if a polymer film is dripped on the reference electrode to prevent the reaction, there will be a small probability that the reference electrode will react, which affects the stability of the potential and ultimately the accuracy of sample detection.
Therefore, there is a need for a detection device that will enable the reference electrode to not affect the overall ion concentration detection.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a sample detection device.
In order to realize the utility model discloses a purpose, the utility model discloses a technical scheme as follows:
a sample detection device comprises a shell, a sample adding chamber, a sample detection channel and a calibration liquid channel, wherein the sample adding chamber, the sample detection channel and the calibration liquid channel are all arranged in the shell, and one end of the sample adding chamber is communicated with the sample detection channel through a sample buffer chamber;
an electrode system covering the reaction reagent is arranged in the sample detection channel; and a reference electrode which is not covered with a polymer membrane is arranged in the calibration solution channel.
Further, the other end of the sample addition chamber is communicated with the air bag through a gas passage.
Furthermore, a calibration solution buffer chamber is further arranged on the calibration solution channel, one end of the calibration solution buffer chamber is communicated with the calibration solution bag, and the reference electrode is arranged at the downstream of the calibration solution buffer chamber.
Furthermore, one end of the sample adding chamber is communicated with the sample buffer chamber through a connecting pipeline, and the cross-sectional area of the sample detection channel is smaller than that of the outlet of the sample buffer chamber.
Furthermore, the sample adding chamber is provided with a sample adding port, one end of the gas channel is connected with the air bag, and the other end of the gas channel is communicated with the sample adding port.
Furthermore, the device also comprises a waste liquid pool, wherein the waste liquid pool, the sample detection channel and the calibration liquid channel are communicated with each other.
The detection device further comprises an electrode layer, the reference electrode is arranged in the electrode layer, the electrode layer further comprises a liquid flow in-place judgment electrode and a working electrode which are arranged independently, the liquid flow in-place judgment electrode, the reference electrode and the working electrode are integrated on the surface of the same base material, the insulating layer is positioned above the electrode layer, and the base material is arranged in the shell; reagent windows are formed in the insulating layer, and a reaction reagent is correspondingly covered above each reagent window.
Further, the liquid flow judgment in-place electrode corresponds to a liquid flow judgment in-place electrode site, the reference electrode corresponds to a reference electrode site, the working electrode corresponds to a working electrode site, and the liquid flow judgment in-place electrode site, the reference electrode site and the working electrode site respectively correspond to a reagent window.
Further, the liquid flow judgment target electrode site, the reference electrode site and the working electrode site are all attached to the insulating layer through screen printing.
Further, the reagent windows are uniformly arranged.
Further, the insulating layer is a UV-cured insulating layer.
Compared with the prior art, the beneficial effects of the utility model are specifically embodied in that:
the utility model discloses the reference electrode that will not drip the polymer film establishes and carries out independent reference in the calibration solution passageway, can the independent detection index of independent combination to can the electric potential of remain stable, also can not receive the interference when the testing sample.
Drawings
FIG. 1 is a schematic structural diagram of a detection device;
FIG. 2 is an exploded view of the electrode system of FIG. 1;
reference numerals: 1-a shell; 2-a sample port; 3-a sample addition chamber; 4-connecting a pipeline; 5-a sample buffer chamber; 6-a sample detection channel; 7. a gas conduit; 8-an electrode system; 9-a calibration liquid channel; 11-a reference electrode; 12-an air bag; 13-calibration solution buffer chamber; 14-a calibration liquid bag; 15-waste liquid pool; 21-a substrate; 22-an electrode layer; 23-an insulating layer; 24-a reactive agent; 25-reagent window; 26-a working electrode; 28-flow judgment to the bit electrode.
Detailed Description
In order to make the technical solution and purpose of the present invention clearer, the technical solution of the present invention will be clearly and completely described below with reference to the embodiments.
Example 1
The sample detection apparatus shown in fig. 1 and 2 includes a housing 1, a sample addition chamber 3, a sample detection channel 6, and a calibration liquid channel 9. The sample adding chamber 3, the sample detection channel 6 and the calibration liquid channel 9 are all arranged in the shell 1, one end of the sample adding chamber 3 is communicated with the sample detection channel 6 through the sample buffer chamber 5, and the other end is communicated with the air bag 12 through the gas channel 7; an electrode system 8 for covering the reaction reagent is arranged in the sample detection channel 6; the detection device also comprises an electrode layer 22, the reference electrode 11 is arranged in the electrode layer 22, and the reference electrode 11 which is not covered with the polymer membrane is arranged in the calibration liquid channel 9. Wherein, the sample adding chamber 3 is provided with a sample adding port 2, one end of the gas channel 7 is connected with the gasbag 12, and the other end is communicated with the sample adding port 2. The loading chamber 3 is configured to load various biological samples from the loading port 2, and the sample detection channel 6 is configured with an electrode system 8, wherein the electrode system 8 can be used to detect various ion concentrations, such as sodium ion, potassium ion, calcium ion, magnesium ion, chloride ion, and the like.
The sample detection channel 6 may also be provided with a sample buffer chamber 5, and a small connecting channel 4 is provided between the sample buffer chamber 5 and the sample chamber 3, which is designed to not allow free flow of sample from the sample addition chamber 3 into the sample buffer chamber 5, and once sample is added to the sample buffer chamber 5 from the sample addition port 2, the sample must be driven from the sample chamber 3 to the sample detection channel 6 by means of external driving. And one end of the sample adding chamber 3 is communicated with a sample buffer chamber 5 through a connecting pipeline 4, and the cross-sectional area of the sample detection channel 6 is smaller than that of the outlet 5 of the sample buffer chamber.
The sample detection device further comprises a calibration liquid channel 9, the calibration liquid channel 9 is used for allowing calibration liquid to pass through, the calibration liquid channel 9 is communicated with the sample detection channel 6, and the calibration liquid sequentially flows through the calibration liquid channel 9, a part of the sample detection channel 6 and the electrode system 8. Reference electrode 11 is also disposed in calibration solution channel 9, and when the calibration solution flows through reference electrode 11, the potential of reference electrode 11 can be monitored and instrument calibration can be realized.
The calibration solution channel 9 may be further provided with a calibration solution buffer chamber 13, one end of the calibration solution buffer chamber 13 is communicated with a calibration solution bag 14, the reference electrode 11 is disposed in a downstream region of the calibration solution buffer chamber 13, and a cross-sectional area of a channel between a downstream of the reference electrode 11 and the sample detection channel 6 is smaller than a cross-sectional area of an outlet of the calibration solution buffer chamber 13, which is designed for allowing the calibration solution to completely cover the reference electrode 11, so that a calibration result is more accurate.
The sample detection device can also be provided with a calibration liquid bag 14, and the calibration liquid bag 14 is communicated with the calibration liquid channel 9. The calibration solution bag 14 is not necessarily disposed on the detecting device, but may be separated from the detecting device, for example, the calibration solution is disposed in the apparatus, and in use, a portion of the calibration solution is quantitatively sucked through the calibration solution channel 9 each time to complete the calibration process.
The sample detection device can also be provided with an air bag 12 which is communicated with the sample adding chamber 3 and used for driving a sample in the sample adding chamber 3 to enter the sample detection channel 6, and can also be provided with a waste liquid pool 15, wherein the waste liquid pool 15, the sample detection channel 6 and the calibration liquid channel 9 are communicated with each other, and the calibration liquid is driven to enter the waste liquid pool 15 after each calibration is completed.
The electrode system 8 further comprises a substrate 21, an insulating layer 23 and a reactive agent 24; the electrode layer 22 further comprises a liquid flow determination position electrode 28 and a working electrode 26 which are independently arranged, the reference electrode 11 is independent of the working electrode 26, so that the detection card can achieve the required purpose without dripping a high polymer film, the liquid flow determination position electrode 28, the reference electrode 11 and the working electrode 26 are integrated on the surface of the same substrate 11 to realize an array type, and the insulating layer 23 is positioned above the electrode layer 22; the insulating layer 23 is provided with reagent windows 25, each reagent window 25 is correspondingly covered with a reaction reagent 24, the reagent windows 25 are used for limiting the area of the reaction reagent 24, the liquid reaction reagent 24 is configured in the area of the reagent windows 25 in a liquid-dropping mode, and the reagent windows 25 are uniformly arranged.
The liquid flow judgment in-place electrode 28 corresponds to a liquid flow judgment in-place electrode site, the reference electrode 11 corresponds to a reference electrode site, the working electrode 26 corresponds to a working electrode site, and the liquid flow judgment in-place electrode site, the reference electrode site and the working electrode site respectively correspond to a reagent window 25. And the liquid flow is judged to be in-place electrode sites, the reference electrode sites and the working electrode sites are attached to the insulating layer through screen printing. The insulating layer is preferably a UV-cured insulating layer. The material of the substrate 21 includes one or more of PVC, PU and PET.
The electrochemical biosensor formed by the technical scheme is shown in figure 1, and the array type electrochemical biosensor testing device capable of testing different indexes is prepared by adopting a screen printing technology, screen printing Ag or Agcl electrodes, after drying, dripping reagents with different performances on different electrodes on a screen printing insulating layer by adopting a dispensing technology. The electrode configuration realizes the function of independent reference, and a polymer film does not need to be additionally covered on the reference electrode 11 during detection.
The integrated array type electrochemical biosensor testing device comprises a sodium ion electrode, a chloride ion electrode, a magnesium ion electrode, a potassium ion electrode, a calcium ion electrode, a chloride ion electrode, a reference electrode 11 and the like. The integrated array type electrochemical biosensor is used for testing a whole blood sample, has good accuracy, can be produced in batch and cannot interfere with each other.
Table 1 shows the correlation between the concentration of each ion in a blood sample measured by an integrated array-type electrochemical biosensor measuring device and the measurement result of a commercial instrument, which is calculated by using a one-point calibration method or a two-point calibration method. The test of each sample was repeated several times, and the precision (CV%) was calculated from the standard deviation and the average value, and the results were as follows.
TABLE 1
The experimental result shows that the array type electrochemical biosensor integrated by the technical scheme of the embodiment 1 can realize one-time detection of a plurality of ions in a complex real sample, and has good measurement repeatability, high accuracy and good correlation with a commercial instrument.
The above description is only for the embodiments of the present invention, and the description is specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several changes and modifications can be made, which all fall within the scope of the present invention.
Claims (10)
1. A sample detection device is characterized by comprising a shell, a sample adding chamber, a sample detection channel and a calibration solution channel,
the sample adding chamber, the sample detection channel and the calibration liquid channel are all arranged in the shell, and one end of the sample adding chamber is communicated with the sample detection channel through the sample buffer chamber;
an electrode system covering the reaction reagent is arranged in the sample detection channel; and a reference electrode which is not covered with a polymer membrane is arranged in the calibration solution channel.
2. The apparatus according to claim 1, wherein the calibration solution channel is further provided with a calibration solution buffer chamber, one end of the calibration solution buffer chamber is communicated with the calibration solution bag, and the reference electrode is disposed downstream of the calibration solution buffer chamber.
3. The apparatus according to claim 1, wherein one end of the sample application chamber is connected to the sample buffer chamber via a connecting conduit, and the cross-sectional area of the sample detection channel is smaller than the cross-sectional area of the outlet of the sample buffer chamber.
4. The apparatus according to claim 1, wherein the sample application chamber has a sample application port, a gas channel is disposed between the sample application chamber and the gas bag, and one end of the gas channel is connected to the gas bag, and the other end is communicated with the sample application port.
5. The apparatus according to claim 1, further comprising a waste liquid reservoir, wherein the waste liquid reservoir, the sample detection channel and the calibration liquid channel are connected to each other.
6. The sample detection device according to claim 1, wherein the detection device further comprises an electrode layer, the reference electrode is disposed in the electrode layer, the electrode layer further comprises a liquid flow in-place judgment electrode and a working electrode which are independently disposed from each other, the liquid flow in-place judgment electrode, the reference electrode and the working electrode are integrated on the surface of the same substrate, and the substrate is disposed inside the housing; an insulating layer is arranged above the electrode layer, reagent windows are formed in the insulating layer, and a reaction reagent covers the upper portion of each reagent window correspondingly.
7. The apparatus according to claim 6, wherein the fluid flow determination site corresponds to a fluid flow determination site, the reference electrode corresponds to a reference electrode site, the working electrode corresponds to a working electrode site, and the fluid flow determination site, the reference electrode site and the working electrode site correspond to a reagent window, respectively.
8. The apparatus according to claim 7, wherein the flow judgment target electrode site, the reference electrode site and the working electrode site are attached to the insulating layer by screen printing.
9. The apparatus according to claim 6, wherein the reagent windows are uniformly arranged.
10. The apparatus according to claim 6, wherein the insulating layer is a UV-curable insulating layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221086360.4U CN217587022U (en) | 2022-05-07 | 2022-05-07 | Sample detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221086360.4U CN217587022U (en) | 2022-05-07 | 2022-05-07 | Sample detection device |
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| Publication Number | Publication Date |
|---|---|
| CN217587022U true CN217587022U (en) | 2022-10-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202221086360.4U Active CN217587022U (en) | 2022-05-07 | 2022-05-07 | Sample detection device |
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| CN (1) | CN217587022U (en) |
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- 2022-05-07 CN CN202221086360.4U patent/CN217587022U/en active Active
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