KR20140003663A - Method of preparing sterilization composition having improved microbicidal activity and sterilization method using the composition - Google Patents

Abstract

The present invention relates to a method for preparing a sterilization composition which is capable of maximizing antibacterial activities and to a sterilization method using the same. By following the method for preparing the sterilization composition: excessive use of chlorite and acid is prevented; production of main sterilizing substance is increased so that excellent sterilization composition which maximizes the antibacterial activities can be prepared; and the sterilization composition prepared by the same has drastically high microbicidal activities for various microorganisms so that the sterilization composition can be usefully applied to various objects such as food and tools for food related fields. [Reference numerals] (AA,EE) Concentration (mg/l); (BB,FF) Chlorine dioxide; (CC,GG) Chlorine; (D1) Comparative example 1; (D10) Example 4; (D11) Example 5; (D2) Comparative example 3; (D3) Comparative example 4; (D4) Comparative example 5; (D5) Comparative example 6; (D6) Comparative example 7; (D7) Example 1; (D8) Example 2; (D9) Example 3; (H1) Comparative example 2; (H10) Example 9; (H11) Example 10; (H2) Comparative example 8; (H3) Comparative example 9; (H4) Comparative example 10; (H5) Comparative example 11; (H6) Comparative example 12; (H7) Example 6; (H8) Example 7; (H9) Example 8

Description

Method for producing sterilization composition with improved sterilization and sterilization method using the same {METHOD OF PREPARING STERILIZATION COMPOSITION HAVING IMPROVED MICROBICIDAL ACTIVITY AND STERILIZATION METHOD USING THE COMPOSITION}

The present invention relates to a method for producing a sterilizing composition capable of maximizing the antibacterial effect of a substance and a method for sterilizing using the same.

Acidified chlorite aqueous solution is a widely used disinfectant in the food industry. Acidified sodium chlorite (ASC), which is mainly produced using sodium chlorite (SC), is the most widely used bactericide. It is known that the Codex alimentarious commission has recently determined the use of ASC in a variety of foods, including processed and unprocessed fruits, vegetables, fresh meats, ground meats, seafood, eggs, and sodium chloride (SC) concentrations of 500-1,200 ppm. (pH 2.3-2.9) levels are allowed.

The acidified chlorite aqueous solution may be prepared by adding various acids (citric acid, lactic acid, phosphoric acid, etc.) allowed for use in food, to an aqueous solution containing a salt containing chlorite ions. Oh upon to react by the addition of acid to chlorite aqueous chlorite ions (ClO ₂ ^-) the proton (H ⁺⁾ and chlorite (chlorous acid) in the state semi stabilization (semi-stable) by combining (HClO ₂₎ to create a, created chlorite is ^{_{ClO 2 - (chlorite), ClO}} 3 - (chlorate), ClO 2 (chlorine dioxide), Cl - (chloride) exists in the form of a mixture with decomposition, repeating the synthesis from a variety of materials, such as Since these intermediate products sterilize microorganisms, they should be applied within 1-2 minutes after mixing.

In this regard, US Pat. No. 4,084,747 discloses a method for preparing a composition having bactericidal activity by reacting 2,700 to 3,300 ppm of sodium chlorite with a water-soluble acid. A long time of about 10 minutes is required, and in order to shorten the treatment time by increasing the sterilization effect, a separate process such as ultrasonication is required, and even if it is performed, it takes about 5 minutes.

In addition, as a general method used to prepare an acidified aqueous solution of chlorite, a method of mixing sodium chlorite and an acid in an aqueous solution of a desired concentration separately and mixing them in a volume ratio of 1: 1 is known (FIG. Existing method>), the solution prepared according to this also has a disadvantage that the sterilization effect is not low enough to produce a chlorine dioxide and / or chlorine having a sterilizing action (see Experimental Examples 1 and 2 below).

The acidified chlorite aqueous solution prepared according to the prior art had a problem in that it could not significantly improve the sterilization effect by itself, one of the reasons for the limitation was the sterilization to some extent at a level that does not affect the quality of food, etc. In order to show the effect is considered to be due to the preparation of the solution to meet the appropriate concentration level, therefore, there is a need for a method for producing a good sterilizing composition that can increase the sterilization power without a separate additional process.

Accordingly, the present invention has been made to solve the problems of the prior art, a method for producing a sterilizing composition which can maximize the antibacterial effect of the material by preventing the excessive use of chlorite and acid while increasing the amount of the main sterilizing material and the same It is an object to provide a sterilization method used.

The bactericidal power is determined by chlorine dioxide (ClO ₂ ) and / or chlorine (Cl), which are produced when a mixture of chlorite and acid is reacted, so it is most important to maximize the product of the substance. Focusing on this point, the present inventors have conducted a process of reacting a high concentration of chlorite aqueous solution and a high concentration of an acid solution to produce a maximum amount of the sterilizing material, and then diluting the reaction solution to a desired concentration and pH condition. By preparing a corresponding acidified aqueous solution of chlorite, it was confirmed that a sterilizing composition can be prepared that can maximize the antibacterial effect of the substance by preventing the excessive use of chlorite and acid while increasing the amount of the major sterilizing substance. The invention was completed.

Thus, a preferred embodiment of the present invention, preparing an aqueous acid solution and 40% (w / v) or more of chlorite aqueous solution (chlorite); Mixing the aqueous acid solution and the aqueous chlorite salt solution; And diluting the mixed solution prepared through the mixing step, to provide a method for producing a sterilizing composition having an increased amount of chlorine dioxide and / or chlorine.

In addition, another preferred embodiment of the present invention, preparing a sterilizing composition prepared by the method; And applying the sterilizing composition to the subject.

Hereinafter, the sterilization composition preparation method and the sterilization method using the same of the present invention will be described in detail.

In the present specification, the sterilization target may be a microorganism, and in the present specification, sterilization is used to include all of inhibiting microbial growth and / or decreasing population, destroying or incapacitating microbial cells.

Such microorganisms include all prokaryotes, including any acellular or unicellular (including colony) organisms, including, for example, bacteria (including cyanobacteria and acidophilus), lichens, fungi, fungi, protozoa, viinos, viroids, viruses and Algae, etc., but is not limited thereto.

The acid for preparing the aqueous acid solution may be used without particular limitation as long as the chlorite ions react with the hydrogen ions to produce sterilizing substances such as chlorine dioxide and chlorine when reacted with the aqueous chlorite solution.

For example, an inorganic acid or an organic acid can be used, and preferably a water-soluble one can be used. Examples of the inorganic acid include sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid, sulfamic acid, perchloric acid, chromic acid, boric acid, carbonic acid, sulfurous acid, nitrous acid, perchloric acid, hypochlorous acid, and the like. Cholic acid, succinic acid, maleic acid, malic acid, lactic acid, tartaric acid, citric acid and the like can be used. Preferably, citric acid, which is the lowest price and the highest dissociation constant, may be used, but is not limited thereto.

The acid aqueous solution may be prepared to have a high concentration, preferably 40% (w / v) or more, may be prepared in a saturated solution.

The chlorite aqueous solution may be any aqueous solution containing chlorite ions. The chlorite for preparing the chlorite may be used without particular limitation as long as it is a metal chlorite. Preferably, alkali metal or alkaline earth metal chlorites can be used.

The alkali metal chlorite may be, for example, sodium chlorite or potassium chlorite, and the alkaline earth metal chlorite may include, for example, barium chlorite, calcium chlorite and magnesium chlorite. Preferably, sodium chlorite, which is readily available at low cost, may be used, but is not limited thereto.

A general reaction of an aqueous sodium chlorite solution and an acid to produce a bactericidal substance such as chlorine dioxide is well known in the art, and in addition to the above, those skilled in the art can use various known acids and chlorites suitable for the present reaction, An aqueous solution can be prepared.

As described above, the chlorite aqueous solution may be prepared to have a high concentration, preferably 40% (w / v) or more, and more preferably to a saturated solution.

That is, preferably, the acid saturated aqueous solution and the saturated chlorite aqueous solution may be prepared and reacted, respectively, to maximize the amount of sterilizing substances such as chlorine dioxide and / or chlorine, and thus the sterilizing effect may be significantly increased.

On the other hand, as an aqueous solvent for producing an acid aqueous solution and a chlorite aqueous solution, water can be preferably used.

The acidified chlorite aqueous solution prepared through the mixing step may further include diluting to have an appropriate concentration and pH conditions, and preferably dilute to a final pH of 3.0 or less.

In addition, the concentration of the aqueous solution of chlorite in the acidified aqueous solution of chlorite can be adjusted by appropriate dilution according to the choice of those skilled in the art in consideration of the application target or purpose. For example, when applied to fruits, seafood, meat, etc., the most chlorite concentration in the concentration range allowed for food application in the United States, Canada, Australia, etc. can be prepared with ASC solution of 1200 ppm or less, Since the amount of chlorine dioxide (water) in the food additives is limited to 200 ppm or less effective chlorine, it can be diluted and applied to meet the above conditions when used for sterilization of foods such as fruits and vegetables.

As described above, in the preparation method according to the preferred embodiment of the present invention, unlike the conventional method of preparing each solution at a concentration to be applied to each other and then mixing each solution, each solution is first prepared at a high concentration, preferably a saturated solution or By preparing to a concentration close to this, mixed with each other, and finally diluted to the desired concentration, the amount of each substance can be reduced more than the conventional method, but the reaction efficiency can be further promoted. And accordingly, the amount of chlorine dioxide and / or chlorine produced is significantly increased to be able to produce a sterilizing composition with the maximum sterilization power.

The prepared bactericidal composition may preferably be an acidified chlorite aqueous solution, and may further include one or more kinds of various known bactericides together with the aqueous solution.

In addition, depending on the application form of the composition, it may be further comprising a preferred additive and / or carrier and the like. Application forms of the bactericidal composition of the present invention may be, for example, antimicrobial agents, hygiene agents, disinfectants, fungicides, fungicides and deodorants, and the like, but are not limited thereto. You can choose appropriately.

The bactericidal composition prepared according to the preferred embodiment of the present invention has an excellent bactericidal effect against various microorganisms, preferably various food poisoning bacteria, and the microorganism may preferably include both Gram-negative bacteria and Gram-positive bacteria. .

The gram-negative bacteria for example, Campylobacter Jeju Needle (Campylobacter jejuni ), Yersinia enterocolitica), Chrono bakteo bacteria (Cronobacter spp.), Shigella (Shigella spp.), it can be parahaemolyticus (Vibrio spp.) Escherichia coli (Escherichia coli), or mouse typhoid bacteria (Salmonella Typhimurium), but is not limited thereto. In addition, the Gram-positive bacteria may be, for example, Clostridium (such as Clostridium perfringens or Clostridium). botulinum, etc.), Bacillus spp., Staphylococcus aureus ) or Listeria monocytogenes , but is not limited thereto.

Thus, another preferred embodiment of the present invention provides a method for sterilization using the sterilization composition prepared by the method of the present invention.

The sterilization method may preferably include preparing a sterilization composition prepared by the above-described manufacturing method and applying the sterilization composition to a subject.

The object is not particularly limited, but may be preferably a food, an apparatus used for food processing, or a surface.

The apparatus used for the food processing may be, for example, various apparatuses used for cooking or processing food, or a connecting pipe, ie, a pipeline, etc. for transporting food to a food processing line or packaging line, but is not limited thereto. .

The surface is not particularly limited and may be various surfaces such as, for example, porous concrete, brick, tile, stone, wood, metal, laminate, vinyl, porcelain, granite, and composites commonly found in household applications.

Application of the sterilizing composition is not particularly limited and may be accomplished using conventional methods, for example, by spraying, foaming, injecting or other various methods.

The preferred amount of the composition can be appropriately adjusted according to the type of microorganism, degree of infection, application route, application form, and the like.

According to the method for preparing a sterilizing composition of the present invention, it is possible to prepare an excellent sterilizing composition which can maximize the antibacterial effect of the substance by preventing the excessive use of chlorite and acid, but increasing the amount of the main sterilizing substance produced. The bactericidal composition has a significantly high bactericidal effect against various microorganisms, and thus may be usefully applied to a wide range of objects, including various apparatuses in food and related fields.

Figure 1 schematically shows a method for producing an acidified sodium chlorite (ASC) aqueous solution of Examples and Comparative Examples.
Figure 2 shows the results of measuring the amount of chlorine dioxide, chlorine produced by pH conditions of Examples and Comparative Examples according to Experimental Example 1.
Figure 3 shows the results of measuring the bactericidal power for various microorganisms of Examples and Comparative Examples according to Experimental Example 2 (treatment time: 1 minute).
Figure 4 shows the results of measuring the bactericidal power for various microorganisms of Examples and Comparative Examples according to Experimental Example 2 (treatment time: 3 minutes).
Figure 5 shows the results of measuring the bactericidal power for various microorganisms of Examples and Comparative Examples according to Experimental Example 2 (treatment time: 5 minutes).

Hereinafter, preferred embodiments and experimental examples are provided to facilitate understanding of the present invention. However, the following examples and experimental examples are provided only to more easily understand the present invention, but the scope of the present invention is not limited thereto.

< Example  1-10 and Comparative Example  1-12>

In order to prepare an acidified sodium chlorite (ASC) aqueous solution, sodium chlorite (SC) (technical grade, 80%, Aldrich, St. Louis, MO., USA) and citric acid (citric) acid, CA) (ACS reagent,> 99.5%, Aldrich) was mixed with sterile secondary distilled water immediately before use to prepare an aqueous sodium chlorite solution and an aqueous citric acid solution.

Specifically, as shown in Table 1, various concentrations of CA (solubility: 75.0 g / 100 ml at 20 ° C) 0.0005, 0.005, 5, 10, 20, 40, 70% (w / v) aqueous solution, and SC (Solubility: 39.0 g / 100 ml at 17 ° C.) 0.00001, 5, 10, 20, 40% (w / v) aqueous solutions were prepared, respectively.

After each of the aqueous solution was mixed in three ways as shown in Figure 1 to prepare an ASC aqueous solution of SC final concentration of 10 ppm, pH 2.5 (or pH 3.0). Each method is as follows. 1) Mixing the aqueous chlorite solution prepared at the concentration to be finally obtained and the aqueous acid solution prepared at the desired pH in a 1: 1 volume ratio (conventional method; C) (Comparative Examples 1 and 2), 2) A method of acidifying to a desired pH by adding a high concentration of aqueous acid solution to the aqueous solution of chlorite prepared to the desired concentration (acidification method; A) (Comparative Examples 3 to 12), and 3) high concentration aqueous solution of chlorite and A high concentration of an aqueous acid solution is prepared, each of which is mixed and reacted, followed by dilution to a desired concentration and pH (mass up method; M) (Examples 1 to 10).

ASC manufacturing conditions used in the experiment division Manufacturing method Sodium chlorite aqueous solution Citric acid Aqueous solution concentration for mixing
(%, w / v) Addition amount
(ml) Final concentration in ASC solution
(ppm) Aqueous solution concentration for mixing
(%, w / v) Addition amount
(ml) Final concentration in ASC solution
(ppm) Comparative Example 1 C 0.00001 15 10 0.0005 15 500
(pH 3.0) Comparative Example 2 0.005 15 5000
(pH 2.5) Comparative Example 3 A 5 0.3 500
(pH 3.0) Comparative Example 4 10 0.15 Comparative Example 5 20 0.075 Comparative Example 6 40 0.0375 Comparative Example 7 70 0.0214 Comparative Example 8 5 3 5000
(pH 2.5) Comparative Example 9 10 1.5 Comparative Example 10 20 0.75 Comparative Example 11 40 0.375 Comparative Example 12 70 0.214 Example 1 M 5 0.006 5 0.3 500
(pH 3.0) Example 2 10 0.003 10 0.15 Example 3 20 0.0015 20 0.075 Example 4 40 0.00075 40 0.0375 Example 5 40 0.00075 70 0.0214 Example 6 5 0.006 5 3 5000
(pH 2.5) Example 7 10 0.003 10 1.5 Example 8 20 0.0015 20 0.75 Example 9 40 0.00075 40 0.375 Example 10 40 0.00075 70 0.214

< Experimental Example >

Experimental Example  1. Each solution pH Chlorine Dioxide, Chlorine Production

The pH of each ASC solution prepared according to the above Examples and Comparative Examples was measured using a pH meter (MP 200, Mettler-Toledo LTD., Seoul, Korea), and also for the measurement of chlorine dioxide and chlorine production in the solution. Colorimeter (DR / 800 colorimeter, HACH, Loveland, Colorade, USA) DPD method (EPA test method registration number: 10126) was used. The measurements were performed according to the manufacturer's manuals respectively. The pH, chlorine dioxide and chlorine production amount of each solution measured according to the above method is shown in Table 2 and FIG. 2.

Chlorine Dioxide and Chlorine Contents by Comparative Examples and Examples Chlorine Dioxide Concentration (mg / l) Chlorine Concentration (mg / l) Comparative Example 1 0.05 ± 0.02 0.15 ± 0.06 Comparative Example 2 0.04 0.01 0.11 ± 0.04 Comparative Example 3 0.05 ± 0.03 0.13 ± 0.08 Comparative Example 4 0.06 ± 0.03 0.27 ± 0.06 Comparative Example 5 0.04 ± 0.03 0.12 ± 0.07 Comparative Example 6 0.07 ± 0.03 0.20 ± 0.06 Comparative Example 7 0.07 ± 0.02 0.17 ± 0.05 Comparative Example 8 0.03 ± 0.01 0.08 ± 0.02 Comparative Example 9 0.05 ± 0.01 0.05 ± 0.05 Comparative Example 10 0.03 ± 0.02 0.06 ± 0.03 Comparative Example 11 0.01 ± 0.01 0.03 ± 0.03 Comparative Example 12 0.05 ± 0.01 0.05 ± 0.06 Example 1 0.10 ± 0.02 0.28 ± 0.05 Example 2 0.11 ± 0.04 0.30 ± 0.11 Example 3 0.26 ± 0.02 0.71 ± 0.04 Example 4 1.03 ± 0.06 2.69 ± 0.15 Example 5 1.94 ± 0.14 5.05 ± 0.37 Example 6 0.06 ± 0.01 0.16 ± 0.01 Example 7 0.13 + 0.02 0.34 ± 0.06 Example 8 0.29 ± 0.02 0.79 ± 0.04 Example 9 0.98 ± 0.07 2.51 ± 0.14 Example 10 1.91 ± 0.05 5.04 ± 0.11

As shown in Figure 2 and Table 2, the comparative example prepared according to the conventional method, that is to prepare a chlorite aqueous solution and an acid aqueous solution at the concentration and pH to be finally obtained and then mix them in a 1: 1 volume ratio In the case of the ASC solutions of Comparative Examples 3 to 12 prepared through the method of acidifying to the desired pH by adding an acidic solution of high concentration to the ASC solution of 1 and 2 and the aqueous chlorite solution prepared at the final concentration, Chlorine and chlorine production was found to be very low at all pH conditions.

On the other hand, in the case of the ASC solution of Examples 1 to 10 of the present invention prepared according to the method of preparing a high concentration aqueous solution of chlorite and an aqueous acid solution and mixing and reacting each of them, and then diluted to a desired concentration and pH, chlorine dioxide and It was confirmed that the amount of chlorine produced was significantly higher than that of the comparative example, and in particular, the solution of Examples 6 to 10 prepared with a solution of pH 2.5 has a higher concentration of chlorine dioxide and chlorine than the solution of Examples 1 to 5 It was found that the lower the final pH, the more the production amount increased. In addition, the chlorine dioxide and chlorine production of Examples 4, 5, 9, and 10 in which the concentrations of the chlorite and acid solutions for preparing the ASC solution were all 40% (w / v) or higher. It was found that the higher the initial concentration of chlorine dioxide and chlorine production increased.

Experimental Example  2. Comparison of the sterilizing power of each solution against various harmful microorganisms

Representative harmful microorganisms were selected to compare the sterilizing power of each solution, and the list is shown in Table 3 below.

Bacteria used in bactericidal test Gram classification Fungus Strain Gram voice Escherichia coli O157: H7) ATCC 35150
ATCC 43889
ATCC 43890 Gram voice Salmonella Typhimurium ATCC 19585
ATCC 43174
DT 104 killercow Gram positive Staphylococcus aureus ) ATCC 23235
ATCC 25923
NRS 111 Gram positive Listeria monocytogenes ) ATCC 19111
ATCC 19115
ATCC 19117

For the three species selected as shown in Table 2, three strains were selected for each species and inoculated in Tryptic Soy Broth (Difco, Detroit, MI, USA), respectively, and incubated at 37 ° C for 24 hours. . Each cultured strain was mixed in 50 ml plastic tubes (Becton Diskinson, Franklin Lakes, NJ, USA) and centrifuged at 3,000 × g for 15 minutes (Centra-CL2, Needham Heights, MA, USA). After decanting the supernatant, the same amount of 0.85% (w / v) sterile saline was added to wash the microbial cells twice to prepare a cell suspension for each cell type.

After inoculating each of the prepared bacterial strains at the level of 7-8 log CFU / ml in each of the ASC solutions prepared according to the Examples and Comparative Examples, the bacteria were collected at 1, 3, and 5 minutes, respectively. Plated on enrichment Tryptic Soy Agar (Dicfo) (detection limit: 1 CFU / ml). The plated medium was colonized on the medium after incubation for 24 hours at the optimum incubation temperature ( E. coli O157: H7, S. Typhimurium, S. aurues: 37 ° C; L. monocytogenes: 30 ° C) for detection of each species By counting the bacteria concentration for each condition was calculated as a log value. Based on the initial number of inoculated bacteria (N ₀ ) and the number of treated bacteria (N _t ), the reduction values (log (N _t / N ₀ )) for each species and treatment time were calculated for each ASC solution, respectively. 4 (3-minute process) and FIG. 5 (5-minute process) were shown.

Looking at the bactericidal effect of each species according to the treatment time of the ASC solution of the Examples and Comparative Examples, the solution of Comparative Examples 1 to 12 in the first minute treatment has little or no bactericidal effect even under low pH condition of pH 2.5 for all species It was confirmed that the results were insignificant, and in particular, bactericidal effect was hardly observed for E. coli. On the other hand, the ASC solution prepared according to Examples 1 to 10 was able to confirm that the bactericidal effect against microorganisms was very excellent even when treated in a short time of 1 minute, as shown in Experimental Example 1, for preparing the ASC solution As the initial concentrations of the chlorite aqueous solution and the acid aqueous solution were higher, it was confirmed that the bactericidal effect was further increased (FIG. 3).

Next, look at the effects of increasing the treatment time to 3 minutes and 5 minutes, respectively, ASC solution of Comparative Example 2, Comparative Examples 8 to 12 and Examples 1 to 10 prepared so that the pH of the final ASC solution is 2.5 In the case of increasing the treatment time was confirmed that the overall sterilization effect for each species is higher (Figs. 4b and 5b).

However, even in this case, it was found that the solution of Comparative Example was still ineffective or very low against Staphylococcus aureus and Listeria, and the solution of Comparative Example 1 and Comparative Examples 3 to 7 having a final pH of 3.0 had a treatment time of 3 minutes. Of course, even after 5 minutes the bactericidal effect was still present or very low (FIGS. 4A and 5A). On the other hand, the solution of Examples 1 to 10, unlike the comparative example showed an excellent sterilization effect for all species, it was confirmed that there is a significantly high sterilization effect even when the pH of the final solution is 3.0.

As described above, when the ASC solution was prepared according to Examples 1 to 10, it was found that the bactericidal effect was very excellent against various harmful microorganisms even under relatively high pH of the final solution. In the case of treatment for a long time, the sterilization effect was high, so that it was possible to obtain an excellent sterilization effect for various species in a short time, it was confirmed that the ASC solution significantly improved compared to the conventional method.

Claims (13)

Preparing an acid aqueous solution and at least 40% (w / v) aqueous chlorite solution;
Mixing the acid aqueous solution and the chlorite aqueous solution; And
Diluting the mixed solution prepared through the mixing step,
A method for producing a sterilizing composition in which the amount of chlorine dioxide and / or chlorine is increased.
The method of claim 1,
The acid aqueous solution is prepared to a concentration of at least 40% (w / v).
The method of claim 1,
The acid aqueous solution and chlorite aqueous solution is prepared by a saturated aqueous solution.
The method of claim 1,
The chlorite is at least one selected from the group consisting of sodium chlorite, potassium chlorite, barium chlorite, calcium chlorite and magnesium chlorite.
The method of claim 1,
The acid is an organic acid or an inorganic acid.
The method of claim 1,
The acid is acetic acid, propionic acid, fumaric acid, gluconic acid, succinic acid, maleic acid, malic acid, lactic acid, tartaric acid, citric acid, sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid, sulfamic acid, perchloric acid, chromic acid, boric acid, carbonic acid, sulfurous acid, nitrous acid, perchloric acid and At least one selected from the group consisting of hypochlorous acid.
The method of claim 1,
The mixed solution is diluted to be less than pH 3.0.
The method of claim 1,
The bactericidal composition is a method having antimicrobial activity against gram negative bacteria or gram positive bacteria.
9. The method of claim 8,
The gram-negative bacteria is Campylobacter Jeju Needle (Campylobacter jejuni ), Yersinia enterocolitica ), Cronobacter spp., Shigella spp., Vibrio spp., Escherichia coli coli ), or Salmonella Typhimurium ).
9. The method of claim 8,
The Gram-positive bacteria are Clostridium , Bacillus spp.), Staphylococcus aureus ) or Listeria monocytogenes ).
Preparing a sterile composition prepared by the method of any one of claims 1 to 10;
A sterilization method comprising applying the sterilization composition to a subject.
12. The method of claim 11,
The subject is a food, an appliance used in food processing, or a surface.
The method of claim 12,
The surface is porous concrete, brick, tile, stone, wood, metal, laminate, vinyl, porcelain, granite or composite.

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