KR20130137827A - Composition for the improvement of obesity regulating absorbing protein, lipid and carbohydrate - Google Patents

Abstract

The present invention relates to a composition for the treatment of obesity regulating the absorption of protein, lipid and carbohydrate, more specifically an anti-obesity composition and health functional food for improving exercise performance which contain peptide derived from plants or insects, a lipolytic enzyme inhibitor, and a glycolytic enzyme inhibitor as active components. The composition and the health functional food which contain the peptide derived from plants or insects, the lipolytic enzyme inhibitor, and the glycolytic enzyme inhibitor as the active components, has excellent prevention and treatment effects on obesity and an improvement effect on exercise performance and endurance. The composition is capable of being used for producing treatments for controlling the obesity and functional food for improving strength. [Reference numerals] (AA) Weight, g;(BB) Dose days;(CC) Normal feed;(DD) High fat diet;(EE) High fat diet+anti-obesity composition

Description

Composition for the improvement of obesity regulating absorbing protein, lipid and carbohydrate}

The present invention relates to a composition for improving obesity that controls protein, lipid, and carbohydrate absorption, which can maintain body balance while enhancing energy while eliminating stamina and rapid aging due to excessive calorie restriction.

Obesity is a state in which excess energy is accumulated in the body due to high energy consumption compared to energy consumption. According to the 2005 National Health and Nutrition Examination Survey, 31.8% of adults over 20 years of age were obese and 14.8% (Ministry of Health & Welfare, 2006. The third Korea National Health and Nutrition Examination Survey). In line with this increase, socioeconomic losses due to obesity are increasing every year, and the government's Comprehensive National Health Promotion Plan has set the main goal of maintaining the adult obesity rate at 30% by 2010 (Ministry of Health and Welfare. 2005. National Health Promotion Plan, pp. 61-64).

In addition, obesity is not only a problem of its own, but also because it can cause diseases such as cardiovascular disease, diabetes, respiratory disease, osteoarthritis, etc., a lot of attention is paid to obesity worldwide (Antipatis VJ et al., 2001, Obesity as a global problem.In International textbook of obesity.Per Bjorntorp, ed.John Wiley & Sons Ltd, Chichester, UK.p3-22), the World Health Organization (WHO) treats obesity as a global nutrition problem. It is perceived as a disease to be treated rather than a simple risk factor (World Health Organization. 1998. Obesity: Preventing and managing the global epidemic.Report of WHO Consultation on Obesity, Geneva). Obesity is due to a combination of excessive energy consumption, lack of exercise, as well as neurological secretion factors, drug causes, genetic factors, etc. As a way to improve obesity, diet, exercise, behavioral therapy or drug treatment is used.

In recent years, research on the development of anti-obesity materials using natural substances with better stability than conventional compounds for the development of obesity treatments has been increasing. According to the research results, hydroxycitric acid is a substance involved in appetite suppression by action mechanism. (HCA), Olibra, chicory, inulin, and the like, and research reports suggest that chitosan and flavonoids may be used as substances that inhibit the digestion and absorption of fat. In addition, there are capsaicin of red pepper, catechin of green tea, retinoic acid, and so on, which inhibit fat accumulation by inducing heat generation, L-carnitine, conjugated linoleic acid (CLA), which regulates lipid metabolism, milk calcium and related proteins, etc. This is being reported. In addition, it was reported that herbal medicines such as fruit, yangja, deficiency, green tea, pine needles, hwahwa, cheongung, and filthy milk were effective for weight control (Kim MH, 2004, Korean J. Heath Psychol. 9: 493-). 509, Reddy P. et al., 1998, Formulary 33: 943-959, Burns AA et al., 2002, Eur. J. Clin.Nutr. 56: 368-377, Delzenne NM et al., 2005, Br. J. Nutr. 1: 157-161, Zacour AC et al, 1992, J. Nutr.Sci.Vitamilol. 38: 609-613, Griffiths DW et al., 1986, Adv.Exp.Med.Biol. 1999: 509 -516).

Therefore, consumers are less interested in these side effects, on the contrary, more attention to natural obesity treatments with excellent efficacy, the development of natural medicines for the treatment of new obesity to meet these needs.

Republic of Korea Patent Publication 10-2009-0125758

It is an object of the present invention to provide a novel anti-obesity composition that effectively inhibits obesity and has no side effects, and has an anti-obesity composition comprising a plant or insect-derived peptide, a lipase inhibitor, and a glycolysis inhibitor, which are not meat; To provide.

In addition, another object of the present invention is to provide a health functional food for enhancing athletic ability, which comprises a non-food plant or insect-derived peptide, a lipase inhibitor, and a glycolysis inhibitor as an active ingredient.

In order to solve the above problems, the present invention provides an anti-obesity composition comprising a plant or insect-derived peptide, a lipase inhibitor and a glycolysis inhibitor as an active ingredient.

In one embodiment of the present invention, the plant or insect-derived peptide may be one or more protein degradation products selected from the group consisting of silk protein, pupa protein and soy protein.

In one embodiment of the invention, the lipase inhibitor may be a cocoa extract.

In one embodiment of the present invention, the glycolysis inhibitor may be a kidney bean extract or Ganoderma lucidum extract.

In another aspect, the present invention provides a health functional food for enhancing exercise ability comprising a plant or insect-derived peptide, a lipase inhibitor and a glycolysis inhibitor as an active ingredient.

In one embodiment of the present invention, the athletic ability may be to enhance endurance.

In one embodiment of the present invention, the plant or insect-derived peptide may be one or more protein degradation products selected from the group consisting of silk protein, pupa protein and soy protein.

In one embodiment of the invention, the lipase inhibitor may be a cocoa extract.

In one embodiment of the present invention, the glycolysis inhibitor may be a kidney bean extract or Ganoderma lucidum extract.

It relates to an anti-obesity composition comprising a plant or insect-derived peptide, an lipase inhibitor and a glycolysis inhibitor as an active ingredient and a health functional food for enhancing exercise ability. Compositions and health functional foods comprising plant or insect-derived peptides, lipolytic inhibitors and glycolytic inhibitors according to the present invention as active ingredients are not only excellent in preventing, improving and treating obesity, but also have endurance to exercise ability. The effect that can be enhanced is also excellent. Therefore, there is an effect that can be usefully used in the manufacture of a functional food for improving physical strength and a therapeutic agent for inhibiting obesity with weight control.

1 is a result of showing the weight gain inhibitory effect of the anti-obesity composition of the present invention in a mouse animal model, ● is a group to eat only normal feed, ▼ is a group to eat only high-fat diet, ■ The high fat diet and the anti-obesity composition of the present invention are shown to be ingested together.
Figure 2 is a graph showing the results of measuring the endurance of each mouse group by checking the swimming time for the mouse group ingested normal diet, high fat diet and high fat diet and the anti-obesity composition of the present invention.

The present invention relates to a novel anti-obesity composition, and more particularly, to an anti-obesity composition comprising a plant or insect-derived peptide, an lipase inhibitor, and a glycolysis inhibitor as an active ingredient.

Plant or insect-derived peptides in the active ingredient contained in the composition according to the present invention serves as a protein source, it may be used one or more selected from the group consisting of silk protein, pupa protein and soy protein.

Conventional body shape management program using chicken breast as a protein source with exercise prescription for the prevention and improvement of obesity is widely applied, animal protein such as chicken breast may have a bad effect on the cardiovascular system as a high calorie.

Therefore, in the present invention, the plant or insect-derived peptide was used as a plant or insect-derived protein excluding an animal protein.

In addition, the plant or insect-derived peptide used in the present invention may use silk protein, chrysalis protein and soy protein itself, and preferably, degradation products of these proteins may be used, wherein the protein degradation products may be selected from acids or alkalis. It may be a peptide obtained by hydrolysis or enzymatic hydrolysis using.

Hydrolysis using an acid or an alkali can be performed by adding an acid or a base and undergoing a process such as heat treatment, and enzymatic hydrolysis is a protease, a protease FG-f, a flavozyme, or a protease A. , Aloes AP-10, piscalase, papain, bromelain, Protease P, Protease N, Alcalase 2.4L, pisin and nutracere use any one or more selected from the group consisting of, but not limited to . Thus, the plant or insect-derived peptides according to the present invention inhibit the maturation and triglyceride synthesis of adipocytes, while suppressing and reducing weight gain, conserving glycogen as a source of energy, alleviating muscle damage, and increasing muscle mass, Increasing bone density plays a role in improving stamina.

In addition, the lipase inhibitor which is the second active ingredient contained in the anti-obesity composition according to the present invention may use cocoa extract.

Lipolytic inhibitor, which is one of the components used in the treatment of obesity, acts to inhibit the digestion of fat by strongly inhibiting lipase for a long time, and orlistat component is widely used. However, orlistat blocks the absorption of fat-soluble vitamins A and E, which causes side effects such as night blindness and loss of antioxidant function. In addition, the use as a functional food material as a drug that can not be used in diabetics is limited.

On the other hand, the cocoa extract used in the present invention has a feature that can be usefully used as a functional food material without specific regulation because the cocoa itself is widely used as a favorite food.

In addition, the third active ingredient glycosylase inhibitor contained in the anti-obesity composition of the present invention can be used as an amylase inhibitor kidney beans extract and alpha-glucosidase inhibitor Ganoderma lucidum extract, The extracts may be used alone or in combination.

Excess carbohydrates in carbohydrates are converted to triglycerides and are known to cause obesity, and low-carb diets are prevalent, but excessive carbohydrate intake can cause energy deficiency, concentration, memory and mood deprivation. have. On the other hand, the kidney bean extract and Ganoderma lucidum extract used in the present invention has the effect of preventing and improving obesity by controlling the absorption of fructose which causes obesity while at the same time avoiding energy degradation due to energy deficiency.

In particular, the cocoa extract, kidney bean extract and Ganoderma lucidum extract contained in the composition of the present invention may be obtained by extracting from nature using extraction and separation methods known in the art, the 'extract' defined in the present invention 'Is extracted from cocoa, kidney beans or ganoderma lucidum using a suitable solvent, and includes, for example, both crude extract, polar solvent soluble extract or nonpolar solvent soluble extract.

As a suitable solvent for extracting the extract, any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, but is not limited thereto. For example, purified water and methanol Alcohols containing 1 to 4 carbon atoms, acetone, ether, benzene, chloroform, including ethanol, propanol, isopropanol, isopropanol, butanol, etc. , Various solvents such as ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. Preferably ethanol (alcohol) can be used.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the preparation method of the extract of the present invention, any known method may be used.

Therefore, in the present invention, the extract is a concept including all the extracts, fractions and purified products obtained at each step of extraction, fractionation or purification, their dilutions, concentrates or dried products.

 On the other hand, the anti-obesity composition comprising a plant or insect-derived peptides, lipase inhibitors and glycolysis inhibitors as an active ingredient has an excellent effect of preventing and inhibiting obesity, according to an embodiment of the present invention, As a result of ingesting the anti-obesity composition of the present invention in a mouse model in which obesity-induced diet was ingested, it was shown that weight gain was significantly suppressed and reduced compared to a group of mice fed only high-fat diets (FIG. 1).

In addition, the anti-obesity composition of the present invention has the effect of improving the exercise capacity as well as the effect of inhibiting obesity.

According to an embodiment of the present invention, swimming in a group of mice fed with a high fat diet only obesity-induced, a group of mice fed a high fat diet and the composition of the present invention and a group of mice fed a normal diet As a result, the group ingested the composition of the present invention showed significantly better endurance improvement effect compared to the group fed normal feed and high fat diet (see FIG. 2).

Therefore, the present inventors have found that the anti-obesity composition of the present invention has the effect of preventing obesity while preventing energy loss and rapid aging due to calorie restriction and enhancing exercise power. In other words, the composition of the present invention was found to be excellent in obesity inhibitory activity without inhibiting exercise ability.

Therefore, the present invention can provide an anti-obesity composition comprising a plant or insect-derived peptide, an lipase inhibitor and a glycolysis inhibitor as an active ingredient, wherein the composition is a pharmaceutical composition or obesity that can prevent and treat obesity It can be used as a food composition for prevention and improvement.

When the composition according to the present invention is used as a pharmaceutical composition, the composition may include only a pharmaceutically effective amount of the active ingredient or may include one or more pharmaceutically acceptable carriers, excipients or diluents. The pharmaceutically effective amount in the above means an amount sufficient to prevent, ameliorate and treat obesity symptoms.

The pharmaceutically effective amount according to the present invention is 0.5 to 100 mg / day / kg body weight, preferably 0.5 to 5 mg / day / kg body weight of the active ingredient. However, the pharmaceutically effective amount may be appropriately changed depending on the degree of obesity symptoms, the age, weight, health condition, sex, route of administration and duration of treatment of the patient.

Also, the pharmaceutically acceptable hereinabove refers to a composition that is physiologically acceptable and does not normally cause an allergic reaction such as gastrointestinal disorder, dizziness, or the like when administered to a human. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. Further, it may further include a filler, an anticoagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent and an antiseptic agent.

In addition, the compositions of the present invention may be formulated using methods known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal. The formulations may be in the form of powders, granules, tablets, emulsions, syrups, aerosols, soft or hard gelatine capsules, sterile injectable solutions, sterile powders.

The composition for the prevention and improvement of obesity symptoms according to the present invention can be administered through various routes including oral, transdermal, subcutaneous, intravenous or intramuscular, and the dosage of the active ingredient is determined by the route of administration, age, sex, weight and It may be appropriately selected depending on various factors such as the severity of the patient. In addition, the composition for the prevention and improvement of obesity symptoms of the present invention can be administered in parallel with a known therapeutic agent (for example, a compound) having the effect of preventing, improving or treating obesity symptoms.

Furthermore, the composition of the present invention contains ingredients derived from nature as an active ingredient, so there is no toxicity and side effects on the body, so it can be used with confidence even during long-term use.

Therefore, the anti-obesity composition of the present invention can be added to food for the purpose of prevention and improvement of obesity symptoms, the composition of the present invention can be used as a composition for food for the prevention and improvement of obesity symptoms.

Therefore, the composition for food for the prevention and improvement of obesity symptoms of the present invention can be easily utilized as a food, such as the main ingredients, side ingredients, food additives, functional foods or beverages that are effective in the prevention and improvement of obesity symptoms.

As used herein, the term “food” refers to a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through a certain processing step, It includes all foods, food additives, functional foods and drinks.

Foods to which the composition for preventing and improving obesity symptoms according to the present invention can be added include, for example, various foods, beverages, gums, teas, vitamin complexes, functional foods, and the like. In addition, in the present invention, the food may include special nutritive foods (e.g., crude oil, spirits, baby food, etc.), meat products, fish meat products, tofu, mackerel, noodles (Such as soy sauce, soybean paste, kochujang, mixed potatoes), sauces, confectionery (eg, snacks), candies, chocolate, gums, ice cream, milk products (eg, fermented milk, cheese, But are not limited to, pickled foods (various kinds of kimchi, pickles, etc.), beverages (e.g., fruit drinks, vegetable beverages, beverages, fermented beverages and the like) and natural seasonings (e.g. The food, beverage or food additive may be prepared by a conventional production method.

The above-mentioned " functional food " refers to a food group which is imparted with added value to function and express the function of the food by physical, biochemical or biotechnological techniques, or to control the bio-defense rhythm of the food composition, Refers to a food prepared by processing a body so as to sufficiently express the body's control function on the body, such as recovery, and the like. Specifically, it may be a health functional food. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.

In addition, in the present invention, the "beverage" refers to a generic term for drinking to quench thirst or to enjoy a taste and includes a functional drink. The beverage contains, as an essential ingredient, a composition for the prevention and improvement of obesity symptoms as an essential ingredient, and there are no particular restrictions on the other ingredients, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Can be.

Furthermore, in addition to the above, foods containing compositions for the prevention and improvement of obesity symptoms of the present invention include various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, such as colorants and fillers (cheese) , Chocolate and the like), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The components can be used independently or in combination.

In the food containing the composition for the prevention and improvement of obesity symptoms of the present invention, the amount of the composition according to the present invention may comprise from 0.001% to 90% by weight of the total food weight, preferably from 0.1% by weight to 40% by weight, and in the case of a beverage, it may be included in a ratio of 0.001 g to 2 g, preferably 0.01 g to 0.1 g, based on 100 ml, for health and hygiene purposes or to control health. In the case of long-term intake of the target may be less than the above range, the active ingredient is not limited to the above range because it can be used in an amount above the above range because there is no problem in terms of safety.

In addition, the present invention can provide a health functional food for enhancing exercise ability, including a plant or insect-derived peptide, a lipase inhibitor, and a glycolysis inhibitor as an active ingredient, wherein the composition for improving exercise ability includes various nutrients and vitamins. , Flavoring agents such as minerals (electrolytes), synthetic and natural flavoring agents, colorants and fillers (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stable It may contain a topical agent, preservatives, glycerin, alcohol, carbonation agent used in the carbonated beverage and the like.

In addition, the health functional food is as described above.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that these examples are provided so that the scope of the present invention is not limited thereto.

Example

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

< Example  1>

Anti-obesity  Composite composition manufacturing

The present inventors prepared a composite composition containing three natural products as follows to prepare an anti-obesity composition having an obesity inhibitory effect. First, a peptide mixture obtained by hydrolyzing silk protein, pupa protein and soy protein as a plant or insect-derived peptide is mixed with cocoa extract acting as an lipase inhibitor, kidney bean extract acting as a glycolysis inhibitor, and Ganoderma lucidum extract. To prepare an anti-obesity composite composition.

< Experimental Example  1>

According to the invention Anti-obesity  Analysis of the Effect of Inhibiting Weight Gain on Complex Compositions

In order to confirm whether the anti-obesity complex composition of the present invention prepared in Example 1 actually has a weight-inhibiting effect, the anti-obesity complex composition of the present invention was added to the feed of the obese-induced mouse model. Then, the change in body weight was measured. At this time, as the control group, the obesity-induced mouse group which continuously consumed the high fat diet instead of the feed to which the anti-obesity complex composition was added and the mouse group that ingested the normal feed from the beginning were used. Each mouse group was fed for 30 days with a given feed, and then weighed at 0, 3, 6, 9, 12, 15, 18, 21, 24, 27 and 30 days from the time of breeding.

As a result, as shown in FIG. 1, the group that consumed only the high fat diet was found to continuously increase in weight over time, while the group that consumed the feed added with the anti-obesity complex composition of the present invention. Was significantly reduced in weight gain compared to the group fed high fat diet only. Therefore, the present inventors have found that the anti-obesity composition according to the present invention has the effect of preventing and improving obesity.

< Experimental Example  2>

According to the invention Anti-obesity  Analysis of endurance enhancement effect of composite composition

In order to determine whether the anti-obesity composition of the present invention has an endurance enhancing effect in addition to the anti-obesity effect, the following experiment was performed. The swimming time was checked in the tank for the same mouse experiment group used in Experimental Example 1.

As a result, as shown in Figure 2, the group of mice fed with the anti-obesity composition of the present invention was shown to have the best endurance enhancer effect compared to the group fed only normal diet and high fat diet.

Therefore, the present inventors have found that the anti-obesity composition of the present invention can be used for improving the health function by improving the exercise ability such as improving endurance as well as the effect of inhibiting and improving obesity.

So far I looked at the center of the preferred embodiment for the present invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

An anti-obesity composition comprising a plant or insect-derived peptide, an lipase inhibitor and a glycolysis inhibitor as an active ingredient. The method of claim 1,
The vegetable peptide is an anti-obesity composition, characterized in that at least one protein degradation product selected from the group consisting of silk protein, pupa protein and soy protein. The method of claim 1,
The lipase inhibitor is an anti-obesity composition, characterized in that the cocoa extract. The method of claim 1,
The glycolysis inhibitor is an anti-obesity composition, characterized in that the kidney bean extract or Ganoderma lucidum extract. A health functional food for enhancing athletic ability, comprising a plant or insect-derived peptide, an lipase inhibitor and a glycolysis inhibitor as active ingredients. The method of claim 5,
The exercise ability is a health functional food for enhancing exercise ability, characterized in that to promote endurance. The method of claim 5,
The plant or insect-derived peptide is a healthy functional food for athletic performance, characterized in that at least one protein degradation product selected from the group consisting of silk protein, pupa protein and soy protein. The method of claim 5,
The lipase inhibitor is a health functional food for enhancing athletic performance, characterized in that the cocoa extract. The method of claim 1,
The glycolysis inhibitor is a healthy functional food for athletic performance, characterized in that the kidney bean extract or Ganoderma lucidum extract.

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