KR20130083979A - The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1976 mirna expression - Google Patents

The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1976 mirna expression Download PDF

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KR20130083979A
KR20130083979A KR1020120004626A KR20120004626A KR20130083979A KR 20130083979 A KR20130083979 A KR 20130083979A KR 1020120004626 A KR1020120004626 A KR 1020120004626A KR 20120004626 A KR20120004626 A KR 20120004626A KR 20130083979 A KR20130083979 A KR 20130083979A
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South Korea
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cell line
mir
hsa
endorphin
mirna
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KR1020120004626A
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Korean (ko)
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김승찬
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김승찬
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Priority to KR1020120004626A priority Critical patent/KR20130083979A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Abstract

PURPOSE: A method for increasing the expression of hsa-miR-1976 miRNA and changing a signal transduction by treating neuronal cells with endorphin is provided to have the same effect as endorphin by adding an antagomer or miRNA using hsa-miR-1976 miRNA. CONSTITUTION: A method for mimicking the effect of endorphin and a method for supplementing a specific signal comprise the step of directly treating a neuronal cell line including in vitro cultured SHSy5y cells with hsa-miR-1976 miRNA and treating with an antagomer. A method for suppressing the expression of a specific protein comprises the step of increasing the expression level of hsa-miR-1976 by treating the cell line with endorphin. When endorphin is added, hsa-miR-1976 miRNA is increased and the expression level of the protein is reduced by targeting a certain mRNA which is not identified.

Description

The alteration of signal transduction by applying endorphin Neuronal cell line including SHSy5y cell line with increased hsa-miR-miR-miR--Ri-NR-1976 1976 miRNA expression}

The present invention is a method of controlling the expression of the protein or product while controlling the mRNA of genes that complementarily bind to one of the miRNAs generated during gene expression of endorphins that act on opioid receptors of neurons.

Beta endorphin is a substance that binds to the opioid receptor, and is widely used as an anesthetic to take various pains along with enkephalin and morphine. Beta endorphin is a substance that exists naturally in the human body, unlike morphine, and is one of the neurotransmitters that are often used for controlling stress. However, how the beta endorphin, which acts as a painkiller, acts on the body and its miRNA mechanism is not clear.

Horace H. Loh, L. F. Tseng, Eddie Wei, and Choh Hao Li (1976). "beta-endorphin is a potent analgesic agent". PNAS 73 (8): 2895-2898

A method of regulating signaling systems through miRNAs produced by endorphins processed into neurons.

In the present invention, after treatment with beta endorphin in neurons, we saw the increase and decrease of miRNA appearing and discovered various intracellular signaling materials targeting them.

1. Using this hsa-miR-1976 miRNA, the same effect as endorphin can be obtained by injecting miRNA such as antagomer or sequence.

2. By controlling this hsa-miR-1976 miRNA with the endorphins themselves, it can be the basis for controlling various signal transduction responses.

FIG. 1 Sequence and targeting sequence of hsa-miR-1976

First, the sequence of this miRNA is shown in FIG. This miRNA is increased by the addition of endorphins, reducing the expression of the protein by targeting specific mRNAs that have not yet been identified. This enables the study of the expression regulation of such signal transducing molecules. You can also insert the miRNA directly or add antagomer to it to mimic the effects of endorphins or to compensate for specific signals.

Claims (2)

A method of mimicing the effects of endorphins and complementing a specific signal by directly treating hsa-miR-1976 miRNA or treating antagomer to a neuronal cell line comprising in vitro cultured SHSy5y cells. The method of claim 1, wherein the cell line is treated with endorphins to increase the expression level of hsa-miR-1976 to inhibit the expression of specific proteins.
KR1020120004626A 2012-01-16 2012-01-16 The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1976 mirna expression KR20130083979A (en)

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KR1020120004626A KR20130083979A (en) 2012-01-16 2012-01-16 The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1976 mirna expression

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KR1020120004626A KR20130083979A (en) 2012-01-16 2012-01-16 The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1976 mirna expression

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KR20130083979A true KR20130083979A (en) 2013-07-24

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109706239A (en) * 2018-09-26 2019-05-03 南京市妇幼保健院 A kind of application of miR-1976 in prevention and treatment bacterial vaginosis BV

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109706239A (en) * 2018-09-26 2019-05-03 南京市妇幼保健院 A kind of application of miR-1976 in prevention and treatment bacterial vaginosis BV

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