KR20130083978A - The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1972 mirna expression - Google Patents

The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1972 mirna expression Download PDF

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KR20130083978A
KR20130083978A KR1020120004625A KR20120004625A KR20130083978A KR 20130083978 A KR20130083978 A KR 20130083978A KR 1020120004625 A KR1020120004625 A KR 1020120004625A KR 20120004625 A KR20120004625 A KR 20120004625A KR 20130083978 A KR20130083978 A KR 20130083978A
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cell line
mir
hsa
endorphin
mirna
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김승찬
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김승찬
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Abstract

PURPOSE: A method for increasing the expression of hsa-miR-1972 miRNA and changing a signal transduction system by treating neuronal cells with endorphin is provided to show the same effect as endorphin by adding an antagomer or miRNA using hsa-miR-1972 miRNA. CONSTITUTION: A method for mimicking the effect of endorphin and a method for supplementing a specific signal comprise the step of directly treating a neuronal cell line including in vitro cultured SHSy5y cells with hsa-miR-1972 miRNA or treating with an antagomer. The expression of a specific protein is suppressed by treating the cell line with endorphin and increasing the expression level of hsa-miR-1972.

Description

The alteration of signal transduction by applying endorphin Neuronal cell line including SHSy5y cell line with increased hsa-miR-miR-MIR-MiR- 1972 miRNA expression}

The present invention is a method of controlling the expression of the protein or product while controlling the mRNA of genes that complementarily bind to one of the miRNAs generated during gene expression of endorphins that act on opioid receptors of neurons.

Beta endorphin is a substance that binds to the opioid receptor, and is widely used as an anesthetic to take various pains along with enkephalin and morphine. Beta endorphin is a substance that exists naturally in the human body, unlike morphine, and is one of the neurotransmitters that are often used for controlling stress. However, how the beta endorphin, which acts as a painkiller, acts on the body and its miRNA mechanism is not clear.

Horace H. Loh, L. F. Tseng, Eddie Wei, and Choh Hao Li (1976). "beta-endorphin is a potent analgesic agent". PNAS 73 (8): 2895-2898

A method of regulating signaling systems through miRNAs produced by endorphins processed into neurons.

In the present invention, after treatment with beta endorphin in neurons, we saw the increase and decrease of miRNA appearing and discovered various intracellular signaling materials targeting them.

1. By using this hsa-miR-1972 miRNA, miRNAs such as antagomers or sequences can be added to give the same effect as endorphins.

2. By controlling this hsa-miR-1972 miRNA with the endorphins themselves, it can be the basis for controlling various signal transduction responses.

FIG. 1 Sequence and targeting sequence of hsa-miR-1972

First, the sequence of this miRNA is shown in FIG. When the miRNA is added to endorphins, it is increased and attached to the mRNA of the target molecule to regulate the expression level of the protein. This may facilitate the expression control of such signal transducing molecules. You can also insert the miRNA directly or add antagomer to it to mimic the effects of endorphins or to compensate for specific signals.

Claims (2)

A method of mimic the effects of endorphins and complementing a specific signal by directly treating hsa-miR-1972 miRNA or antagomer to a neuronal cell line comprising in vitro cultured SHSy5y cells. The method according to claim 1, wherein the cell line is treated with endorphins to increase the expression of hsa-miR-1972 to inhibit the expression of specific proteins.
KR1020120004625A 2012-01-16 2012-01-16 The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1972 mirna expression KR20130083978A (en)

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KR1020120004625A KR20130083978A (en) 2012-01-16 2012-01-16 The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1972 mirna expression

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KR1020120004625A KR20130083978A (en) 2012-01-16 2012-01-16 The alteration of signal transduction by applying endorphin neuronal cell line including shsy5y cell line with increased hsa-mir-1972 mirna expression

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KR20130083978A true KR20130083978A (en) 2013-07-24

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