KR20130077079A - Biomarkers for evaluating sediment ecotoxicity of fullerene from aquatic midge, chironomus riparius and the uses thereof - Google Patents

Abstract

PURPOSE: A biomarker for assessing ecotoxicity of fullerene is provided to assess ecotoxicity of novel fullerene with the biomarker obtained from midge, which is an indicator species of which the sequence information is not clarified, and to be able to contribute to preparing for future remedial measures as the biomarker is able to be usefully applicable to accurately determine the ecotoxicity contamination generated by fullerene. CONSTITUTION: A biomarker for assessing ecotoxicity of fullerene includes at least one midge originated gene selected from the group consisting of the base sequence of sequence number 1 to 72. A DNA chip for assessing ecotoxicity of fullerene includes at least one midge originated gene selected from the group consisting of the base sequence of sequence number 1 to 72. A method of detecting the presence of fullerene in a sample comprises: a step of exposing midge to a sample assumed to be polluted by fullerene and isolating RNA from the midge; a step of synthesizing cDNA using the isolated RNA as a template; a step of hybridizing with cDNA by contacting the synthesized cDNA with the DNA chip; a step of detecting the hybridization between the DNA chip and cDNA; and a step of comparing the detected hybridization with positive control.

Description

Biomarkers for Evaluating sediment ecotoxicity of fullerene from aquatic midge, Chironomus riparius and the uses approx}

The present invention relates to invertebrate aquatic for carbon nanomaterial of ecotoxicity evaluation of the fullerene animal kkaldagu biomarkers and the use thereof, and more particularly, one or more kkaldagu is selected from the group consisting of the nucleotide sequence of SEQ ID NO: 1 to 72 (Chironomus riparius ) biomarker for the evaluation of fullerene (fullerene, C60) derived gene, gene chip for fullerene ecological toxicity evaluation, kit for fullerene ecological toxicity evaluation and composition for fullerene ecological toxicity evaluation and the biomarker It provides a method for detecting the presence of fullerene in the sample.

Nanotechnology is actively used in all fields of science as the development of science and technology allows the production of atomic-molecule-sized materials. However, despite the many benefits of nanotechnology, concerns about the harmfulness of nanomaterials have arisen internationally. Toxicity studies on nanomaterials are currently at the starting point, but mainly focused on human toxicity, and since the test method of nanotoxicity studies on soil organisms has not been properly developed, studies on soil organisms have been limited. . This is because nanomaterials are particulate matters unlike conventional water-soluble materials, which makes it difficult to apply standard toxicity test methods. In particular, fullerene, a representative carbon-based nanoparticle, refers to carbon cluster C60 formed by combining 60 carbon elements in the shape of a soccer ball. However, it is used as a resin to increase durability, heat resistance, remove static electricity, and noise filter. Recently, studies on the photoinduced toxicity (Yang et al., Toxicology 16 pp. 41 ~ 46 (2002)) and the possibility of inducing toxicity on aquatic ecosystem have been conducted. (Oberdorster, Environ Health Perspect 112 pp. 1058-1062 (2004)).

Chironomus is one of the most representative insect species in the aquatic ecosystem. The larvae of Suseonggi, which last up to 4th instar, are about 2 ~ 100 ㎜ in size and are an important indicator species of water pollution. They are used as food for fish and predatory invertebrates and birds and occupy an important position in the Suseo ecosystem food chain have. They are benthic organisms living mainly in junitos (sediments) and are easily exposed to accumulated pollutants such as PAHs (polycyclic aromatic hydrocarbons) in contact with pore water or upper layer water in sediments or inhaling sediments And is widely used for sediment toxicity testing. In particular, the introduction of nanomaterials, such as fullerene, a carbon nanoparticle that is representative of aquatic ecosystems, leads to the accumulation of sediment layers and toxic effects on benthic organisms that move between sediment layers and water. This will have an impact on the predators predominantly feeding benthic organisms, leading to the destruction of food chains in the ecosystem, resulting in a reduction in habitats, densities, species diversity, food, and growth of aquatic life. The larvae commonly used in this sediment toxicity test are the larvae of Chironomus riparius and Chironomous tentans , which survive for 10 days, grow rate or 28-day allegory to assess the effects of pesticide treatment. It is widely used for evaluating the number and sex ratio of an adult (Byung-Seok Kim et al., Korean Journal of Pesticide Science, Vol.

Funnel ( Chironomus riparius ) has long been used as an ecotoxicity monitoring model, but there is a limit to sensitive toxicity diagnosis because genetic information is not available. If functional chips are produced by the toxic material mechanism in the funnel, it is expected to make a significant contribution to the ecotoxicity and risk assessment. In addition, the development of a functional chip for fullerene, a carbon nanoparticle, is expected to be applicable to the toxicity of other types of carbon-based nanoparticles.

Korean Patent No. 1088456 discloses a method and kit for assessing environmental risks using an organism's ecotoxicity determination point, and Korean Patent No. 1183666 discloses a 'Songsari Genetic Chip for Environmental Hormone Toxicity'. However, as described in the present invention, no biomarker for ecotoxicological evaluation of fullerene, which is a nanoparticle of carbon material derived from a funnel, has not been found at all.

The present invention has been made in accordance with the above requirements, and the present inventors found that the expression level of specific genes in the fungus larvae belonging to the ecotoxicity indicator organisms exposed to fullerene 1 mg / L were significantly increased or decreased compared to the control without fullerene treatment. By confirming, the present invention was completed.

In order to solve the above problems, the present invention is one or more funnel selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 72 ( Chironomus The present invention provides a biomarker for ecotoxicity assessment of fullerene containing genes derived from riparius ), a gene chip for ecotoxicity assessment of fullerenes, and a kit for ecotoxicity assessment of fullerenes.

The present invention also provides a method for detecting the presence of fullerene in a sample using the biomarker.

In addition, the present invention provides a composition for evaluating the ecotoxicity of fullerenes comprising at least one genera derived gene selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 72.

The present invention provides a biomarker for evaluating ecotoxicity of a fullerene obtained from a fungus, an indicator species for which the sequence information is not yet known, and can be usefully applied to accurately determine ecotoxicity pollution caused by fullerene. It can greatly contribute to making improvement plans.

Figure 1 shows the SOLEXA sequencing method used to secure a specific expression genomic database of the funnel following fullerene treatment.
FIG. 2 shows genotyping of litter cells increased or decreased with fullerene treatment.

In order to achieve the above object, the present invention is a funnel ( Chironomus riparius ) provides a biomarker for evaluating the ecotoxicity of fullerenes (including fullerene).

Derived from the above The gene may be composed of one or more nucleotide sequences selected from the group consisting of the nucleotide sequences of SEQ ID NOs: 1 to 72, but is not limited thereto. In addition, variants of the respective base sequences are included within the scope of the present invention. Specifically, the gene is at least 70%, more preferably at least 80%, even more preferably at least 90%, and most preferably at least one base sequence selected from the group consisting of the base sequences of SEQ ID NOs: 1 to 72, respectively. May comprise base sequences having at least 95% sequence homology. "% Of sequence homology to polynucleotides" is ascertained by comparing the comparison region with two optimally aligned sequences, and a portion of the polynucleotide sequence in the comparison region is the reference sequence for the optimal alignment of the two sequences (I. E., A gap) relative to the < / RTI >

Each gene expression amount consisting of the nucleotide sequence of SEQ ID NOS: 1 to 72 that can be used as the biomarker of the present invention is characterized by a significant increase or decrease compared to the control group not treated with fullerene in vivo of the fungus exposed to fullerene Indicates.

The present invention also provides a gene chip for evaluating the ecotoxicity of fullerenes including the gene for the funnel.

Derived from the above The gene may consist of one or more nucleotide sequences selected from the group consisting of the nucleotide sequences of SEQ ID NOs: 1 to 72, and preferably include all of the nucleotide sequences of SEQ ID NOs: 1 to 72, but is not limited thereto. In addition, variants of the respective base sequences are included within the scope of the present invention, as described above.

Gene chips can be used interchangeably with microarrays. In the microarray of the present invention, "microarray" refers to a microarray in which genes are immobilized at a high density on a substrate, and the genes are immobilized in a predetermined region, respectively. Such microarrays are well known in the art. Microarrays are described, for example, in U.S. Pat. Nos. 5,445,934 and 5,744,305, the contents of which are incorporated herein by reference.

The term "substrate" refers to any substrate to which a gene probe can be coupled under conditions that retain hybridization properties and keep the background level of hybridization low. Typically, the substrate may be a microtiter plate, a film (e.g., nylon or nitrocellulose) or a microsphere (bead) or chip. Before application or fixation to the membrane, the nucleic acid probe may be modified to promote immobilization or improve hybridization efficiency. Such modifications may include homopolymer tailing, coupling with different reactive functional groups such as aliphatic groups, NH ₂ groups, SH groups and carboxyl groups, or coupling with biotin, hapten or protein.

In addition, the present invention provides a kit for evaluating ecotoxicity of fullerenes comprising a gene of rugs.

Derived from the above The gene may consist of one or more nucleotide sequences selected from the group consisting of the nucleotide sequences of SEQ ID NOs: 1 to 72, and preferably include all of the nucleotide sequences of SEQ ID NOs: 1 to 72, but is not limited thereto. In addition, variants of the respective base sequences are included within the scope of the present invention, as described above. The kit may include a reagent that separates RNA from a sample, a reagent that synthesizes cDNA from the separated RNA, and a hybridization reagent.

In addition, the present invention comprises the steps of exposing the funnel to the sample presumed to be contaminated with fullerene and separating RNA from it;

Synthesizing cDNA using the separated RNA as a template;

Contacting the synthesized cDNA with the gene chip to hybridize with the cDNA;

Detecting a degree of hybridization between the gene chip and cDNA; And

It provides a method for detecting the presence of fullerene in a sample comprising the step of comparing the detected degree of hybridization with a positive control.

The method of the present invention is a method for detecting the presence of fullerene in a sample presumed to be contaminated with fullerene, and the positive control group shows the nucleotide sequence of SEQ ID NOs: 1 to 72 of the present invention showing the increase or decrease of gene expression in the funnel exposed to fullerene. Refers to the degree of hybridization. The more similar the hybridization of the positive control group to the hybridization of the test sample, the higher the probability of fullerene in the sample. On the contrary, the less the similarity, the lower the probability of fullerene in the sample.

The funnel of the present invention preferably utilizes a funnel larvae, but is not limited thereto.

In the method for detecting the presence or absence of fullerene of the present invention, a method for separating RNA from a funnel exposed to a sample suspected to be contaminated with fullerene may use a method well known in the art.

As a method for synthesizing the first strand cDNA using the separated RNA as a template, a method commonly used in the art can be used. For example, using a reverse transcriptase, an RNase block ribonuclease inhibitor or the like, cDNA can be synthesized. Examples of reverse transcriptases include reverse transcriptases from various sources, such as avian myeloblastosis virus-derived virus reverse transcriptase (AMV RTase), mouse leukemia virus-derived reverse transcriptase ( murine leukemia virus-derived virus reverse transcriptase (MMLV RTase) and Rous-associated virus 2 reverse transcriptase (RAV-2 RTase).

Preferably, the cDNA can be labeled with a detectable labeling substance. The labeling substance may be a fluorescent, phosphorescent or radioactive substance, but is not limited thereto. Preferably, the labeling substance is Cy5 or Cy3. When the first strand cDNA is synthesized by labeling Cy5 or Cy3 at the 5'-end of the primer, the target sequence may be labeled with a detectable fluorescent labeling substance. When the radioactive isotope such as ³² P or ³⁵ S is added to the reaction solution during the synthesis of the first strand cDNA, the synthetic product is synthesized and radioactive is incorporated into the synthesized product so that the synthesized product can be labeled with radioactivity have.

In addition, the present invention provides a composition for evaluating the ecotoxicity of fullerenes containing the gene of the funnel.

Derived from the above The gene may consist of one or more nucleotide sequences selected from the group consisting of the nucleotide sequences of SEQ ID NOs: 1 to 72, and preferably include all of the nucleotide sequences of SEQ ID NOs: 1 to 72, but is not limited thereto. In addition, variants of the respective base sequences are included within the scope of the present invention, as described above.

The composition comprises one or more genera derived from the group selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 72 as an active ingredient, each of the genes in a control group that is not treated with fullerene in vivo in the incubation exposed to fullerene Compared with the fullerenes, it is possible to evaluate the toxicity of fullerenes.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example  One. Crumple  ( Chironomus riparius Pyro Sequencing to Obtain a Genome Database pyrosequencing )

50 larvae of caterpillars were exposed for 24 hours, lyophilized to extract mRNA, synthesized with cDNA, and used as a representative method of NGS (Next Generation Sequencing) using Pyro Sequencing (Roche 454, GS FLX Titanium Run). Secured the database.

① Preparation of Sample

Four samples were used to extract total RNA using Trizol. 1 ml of TRIzol Reagent was added to 50-100 mg tissue and completely homogenized. After phase separation, total RNA was precipitated, washed, and pellets were washed with RNase-free water. RNase-free water was added so that the final volume of 1 mg of total RNA was 0.5 ml, and then placed in a 65 ° C. heating block for 10 minutes. 3 μl of biotinylated-oligo (dT) probe and 13 μl of 20X SSC were added, then mixed well and cooled slowly at room temperature. Flick the SA-PMPs (Streptavidin-Paramagnetic Particles) tubes to flatten them well, then place the tubes on a magnetic stand to collect the SA-PMPs on the sides of the tubes, then carefully Was removed. In the same manner, SA-PMPs were washed three times using 0.5 × SSC (300 μl). Finally, SA-PMPs were resuspended in 100 μl of 0.5 × SSC. The prepared annealing reaction mixtures were all added to the washed tubes of SA-PMPs and reacted at room temperature for 10 minutes. At this time, inverting (inverting) and mixed every 1-2 minutes. The SA-PMPs tube was placed on a magnetic stand and the supernatant was carefully removed. In the same manner, SA-PMPs were washed four times using 0.1 × SSC (300 μl). Finally, the SA-PMP pellets were rediluted in 100 μl of RNase-free water, and the SA-PMPs tube was placed on a magnetic stand and the supernatant (eluted mRNA) was carefully transferred to a new tube. This process was repeated to obtain 200 μl of total eluted mRNA volume. 0.1 volume of 3M sodium acetate (pH 5.2) and 1.0 volume of isopropanol of the eluted mRNA amount were added and left overnight at −20 ° C., followed by centrifugation at 13,000 rpm and 4 ° C. for 10 minutes. mRNA pellets were washed with 1 ml of 75% ethanol (13,000 rpm, 5 min at 4 ° C.).

② cDNA synthesis

5 μl 10 × first strand buffer, 5 μl 100 mM DTT, 5 μl dNTP (2.5 mM each), 5 μl Oligo d (T) 20 (50 μM), 2.5 μl StrataScript Reverse Transcriptase ( 200 U / μl), xμl (<10μl) poly (A) + RNA (˜5 μg) were added to a final volume of 50 μl. After mixing well, the mixture was centrifuged, reacted at 65 ° C. for 3 minutes, and cooled at room temperature. The reaction was carried out at 42 ° C. for one hour. Sterile water was added to the inactivated first strand cDNA at 70 ° C. for 15 minutes to prepare a total volume of 100 μl, 20 μl 10 × second strand buffer, 6 μl second strand dNTP mixture, and 61 μl sterile water were added. 2 μl RNase H (1.5 U / μl) and 11 μl DNA polymerase I (9.0 U / μl) were further added, followed by reaction at 16 ° C. for 2 hours and 30 minutes, and immediately put on ice. 23 μl blunting dNTP mix and 2 μl cloned Pfu DNA polymerase (2.5 U / μl) were added to the second strand synthesis product, and then rapidly vortexed and reacted at 72 ° C. for 30 minutes. Purification using a QIAquick® PCR Purification Kit (final volume 50 μl).

③ Pyro Sequencing (GS FLX Titanium Run)

Using the synthesized cDNA, a DNA library was prepared and subjected to a PCR procedure (clonal amplification) to attach DNA Capture Beads. The sequencing run sequentially flowed nucleotides into a platform called PicoTiterPlate with millions of wells, and when the nucleotides flowed through PicoTiterPlate, sequencing analysis was performed on sequencing beads with millions of DNA fragments on the surface where clonal amplification occurred. This is done. At this time, if nucleotides complementary to the dNTPs flowing on the surface of the sequencing bead are synthesized by the polymerase, at the same time, light is generated in the synthesized wells, and the sequence of the EST is detected using data after being detected by the CCD camera. Could get

Example  2. SOLEXA  Sequencing ( SOLEXA seqeuncing )through Fullerene  Acquisition of specific expression genome database according to processing

Funnels with fullerene treatment ( Chironomus To obtain a specific expression genome database (DB) of riparius ), 50 rats of 4 stage larvae treated with 1 mg / L fullerene at 20 ° C. for 24 hours and untreated controls, David et al. (David et al. BMC Genomics 2010 11: 216), SOLEXA sequencing was performed. The entire genome list obtained from the previously performed pyro sequencing was examined for the expression of genes due to fullerene exposure.

As shown in FIG. 1, the expression level of the genome was determined by measuring the number at a predetermined portion of the sequence during sequencing. Through this, genes increased or decreased by fullerene were distinguished. Among the 25% of genomes for which genetic information can be obtained, only the genomes showing the tendency to increase or decrease in the treatment group were classified into ontology as shown in FIG. 2. . Confirmation list of fullerene treatment group DEG (Differential Expressed Genes) is shown in Table 1. By comparing Highest Depth values, we selected 72 genes that were meaningful in virulence mechanisms. The cDNA sequence database obtained through sequencing into a file is then converted to another species in the NCBI database or the previously identified Chironomus by the BLASTx method. The accessibility of riparius with its amino acid sequence was confirmed and listed. NCBI blast X search in the database to classify contig with genomic information, of which the sequence length is 500 bp or more, and the list of Highest Depth values of 100 or more, which is considered to have high expression in this species in the treatment group. I figured out. Among the contig showing more than 10% increase and decrease, genes commonly used as stress markers were selected, and among them, 72 genes which can be mainly identified in similar insects were selected as biomarkers.

Name Highest depth up - down ratio Gene Chiro -
control Chiro - C60 contig00006
(SEQ ID NO: 1) 11131 2015 -82% CHK1 checkpoint-like protein
Helicoverpa armigera contig00447
(SEQ ID NO: 2) 980 1490 52% Metallothionein family protein
[Trichomonas vaginalis G3] contig00462
(SEQ ID NO: 3) 2152 3529 64% heat shock cognate 70
[Chironomus yoshimatsui] contig00726
(SEQ ID NO: 4) 5031 4154 -17% ribosomal protein S3
[Chironomus riparius] contig00772
(SEQ ID NO: 5) 214 321 50% heat shock transcription factor (hsf) [Aedes aegypti] contig00835
(SEQ ID NO: 6) 4681 3105 -34% cytochrome b [Culicoides arakawae] contig00840
(SEQ ID NO: 7) 2284 1903 -17% 60S ribosomal protein L7a
[Culex quinquefasciatus] contig00849
(SEQ ID NO: 8) 194 307 58% ATP-citrate synthase [Aedes aegypti] contig00936
(SEQ ID NO: 9) 200 317 59% acyl-CoA oxidase [Culex quinquefasciatus] contig01163
(SEQ ID NO: 10) 3252 4903 51% 40S ribosomal protein S7 [Aedes aegypti] contig01191
(SEQ ID NO: 11) 1280 1528 19% map kinase-interacting serine / threonine kinase [Aedes aegypti] contig01263
(SEQ ID NO: 12) 516 711 38% aldehyde dehydrogenase [Aedes aegypti] contig01343
(SEQ ID NO: 13) 180 44 -76% hemolymph proteinase 19 [Manduca sexta] contig01678
(SEQ ID NO: 14) 10680 13619 28% actin 1 [Nilaparvata lugens] contig02823
(SEQ ID NO: 15) 246 359 46% nucleolar GTP-binding protein
[Aedes aegypti] contig03291
(SEQ ID NO: 16) 2335 1021 -56% larval serum protein 2
[Culex quinquefasciatus] contig03359
(SEQ ID NO: 17) 7389 6076 -18% ribosomal protein Ubq / L40e
[Agriotes lineatus] contig03624
(SEQ ID NO: 18) 1406 1927 37% glutamate dehydrogenase, short peptide [Drosophila melanogaster] contig03983
(SEQ ID NO: 19) 195 335 72% cytochrome P450 [Culex quinquefasciatus] contig04024
(SEQ ID NO: 20) 533 778 46% mitochondrial malate dehydrogenase 2
[Culex quinquefasciatus] contig04162
(SEQ ID NO: 21) 580 795 37% utp-glucose-1-phosphate uridylyltransferase 2 [Culex quinquefasciatus] contig04618
(SEQ ID NO: 22) 6116 3720 -39% NADH dehydrogenase subunit 1
[Anopheles gambiae] contig04622
(SEQ ID NO: 23) 693 453 -35% DNA-binding nuclear protein p8
[Simulium vittatum] contig05549
(SEQ ID NO: 24) 265 422 59% hemolymph proteinase 6 [Manduca sexta] contig06416
(SEQ ID NO: 25) 253 777 207% cytochrome P450 [Aedes aegypti] contig06433
(SEQ ID NO: 26) 477 945 98% glucose-6-phosphate isomerase
[Culex quinquefasciatus] contig06748
(SEQ ID NO: 27) 543 999 84% carboxylesterase [Aedes aegypti] contig07318
(SEQ ID NO: 28) 129 275 113% alcohol dehydrogenase 3
[Chironomus riparius] contig07720
(SEQ ID NO 29) 185 314 70% glutathione S-transferase
[Chironomus tentans] contig07984
(SEQ ID NO: 30) 789 1175 49% mitochondrial NADH: ubiquinone oxidoreductase NDUFV1 / 51 kDa subunit [Aedes aegypti] contig08276
(SEQ ID NO 31) 264 155 -41% glutathione transferase, zeta class (AGAP002898-PA) [Anopheles gambiae str. PEST] contig08697
(SEQ ID NO 32) 248 351 42% aldehyde dehydrogenase [Aedes aegypti] contig09526
(SEQ ID NO: 33) 514 859 67% DNA polymerase [Fowlpox virus] contig10025
(SEQ ID NO 34) 409 554 35% mitochondrial processing peptidase beta subunit [Aedes aegypti] contig10185
(SEQ ID NO: 35) 762 1177 54% 60S ribosomal protein L24 [Aedes aegypti] contig11219
(SEQ ID NO: 36) 429 566 32% vitellogenin [Culex quinquefasciatus] contig13104
(SEQ ID NO: 37) 547 945 73% NADH-ubiquinone oxidoreductase sgdh subunit [Culex quinquefasciatus] contig14290
(SEQ ID NO: 38) 3656 9326 155% hemoglobin 13 [Chironomus thummi] contig20375
(SEQ ID NO: 39) 548 682 24% heat shock protein [Aedes aegypti] contig20379
(SEQ ID NO: 40) 1175 1589 35% cytochrome c oxidase subunit VIIa
[Culex quinquefasciatus] contig20383
(SEQ ID NO: 41) 420 306 -27% cytochrome p450 [Aedes aegypti] contig20408
(SEQ ID NO: 42) 875 1162 33% hemoglobin CTT6 [Polypedilum vanderplanki] contig20500
(SEQ ID NO: 43) 108 396 267% Metallothionein family protein
[Trichomonas vaginalis G3] contig20537
(SEQ ID NO: 44) 2724 3923 44% ribosomal protein S3 [Aedes albopictus] contig20580
(SEQ ID NO: 45) 1222 1544 26% hemoglobin CTT6 [Polypedilum vanderplanki] contig20591
(SEQ ID NO: 46) 543 194 -64% pupal cuticle protein, putative
[Aedes aegypti] contig20678
(SEQ ID NO: 47) 2338 2915 25% ribosomal protein L35
[Culicoides sonorensis] contig20781
(SEQ ID NO 48) 3244 3608 11% ribosomal protein L18a
[Polypedilum vanderplanki] contig20843
(SEQ ID NO: 49) 1944 2489 28% ribosomal protein L3 [Aedes aegypti] contig20854
(SEQ ID NO: 50) 1811 1460 -19% 40S ribosomal protein S24 [Aedes aegypti] contig20895
(SEQ ID NO: 51) 750 585 -22% mitochondrial ATP synthase F chain [Ochlerotatus triseriatus] contig20923
(SEQ ID NO: 52) 972 1157 19% multifunctional chaperone
[Simulium nigrimanum] contig20931
(SEQ ID NO: 53) 2341 1667 -29% 60S ribosomal protein L6 [Aedes aegypti] contig20956
(SEQ ID NO: 54) 499 764 53% alcohol dehydrogenase 3
[Chironomus riparius] contig21096
(SEQ ID NO: 55) 2704 3817 41% heat shock cognate 70 [Chironomus tentans] contig21110
(SEQ ID NO: 56) 2166 4007 85% calcium-transporting atpase sarcoplasmic / endoplasmic reticulum type (calcium pump) [Aedes aegypti] contig21114
(SEQ ID NO: 57) 6822 9858 45% 60S ribosomal protein L8 [Culex tarsalis] contig21172
(SEQ ID NO: 58) 8341 871 -90% putative senescence-associated protein [Cupressus sempervirens] contig21194
(SEQ ID NO: 59) 3221 3940 22% cytochrome b [Chironomus inquinatus] contig21199
(SEQ ID NO: 60) 422 313 -26% heat shock cognate 70 [Aedes aegypti] contig21217
(SEQ ID NO: 61) 811 979 21% cytochrome C oxidase subunit IV-like protein [Phlebotomus papatasi] contig21230
(SEQ ID NO: 62) 619 278 -55% zinc carboxypeptidase [Aedes aegypti] contig21269
(SEQ ID NO: 63) 6079 8711 43% putative ribosomal protein S4e [Graphocephala atropunctata] contig21299
(SEQ ID NO: 64) 7022 4576 -35% cytochrome oxidase subunit II
[Chironomus calligraphus] contig21301
(SEQ ID NO: 65) 3372 3876 15% 40S ribosomal protein S11
[Culex quinquefasciatus] contig21315
(SEQ ID NO: 66) 7786 9238 19% ribosomal protein rps23 [Arenicola marina] contig21328
(SEQ ID NO: 67) 1437 1700 18% heat shock cognate 70 [Chironomus tentans] contig21482
(SEQ ID NO: 68) 3864 2604 -33% ribosomal protein L9 [Culicoides sonorensis] contig21542
(SEQ ID NO: 69) 898 1124 25% aldehyde dehydrogenase
[Culex quinquefasciatus] contig21550
(SEQ ID NO: 70) 1114 1436 29% malate dehydrogenase
[Culex quinquefasciatus] contig21554
(SEQ ID NO: 71) 784 1096 40% alcohol dehydrogenase 3
[Chironomus riparius] contig21823
(SEQ ID NO: 72) 2751 1743 -37% 60S ribosomal protein L22
[Culex quinquefasciatus]

<110> University of Seoul Industry Cooperation Foundation <120> Biomarkers for evaluating sediment ecotoxicity of fullerene from aquatic midge, Chironomus riparius and the uses <130> PN11221 <160> 72 <170> Kopatentin 2.0 <210> 1 <211> 2249 <212> DNA <213> Chironomus riparius <400> 1 acgctcgaca gtttgtatgc cagctgtggt gagagtgtgt gaaaaaaatt tttttttgtt 60 tgcacacttt taaaatatgg gtcgaaagat gaagaaagtc gtgtcaatgc caacggttgg 120 ttggtgtgtc attgatgagt atcagtaata gttaatgttt aaattttttg atatagagaa 180 gtgtaatgca ctgaatgtat atgtggaata gtatttaggt atgaggtgta ttaggttgat 240 ggttgtgaca cacacaatgc ataagactat gtacatttgt aattttattg caagttgtat 300 atatgatgat atgtagaaga aaaaaaaaat gtgagagaat tctggttgat cctaccagta 360 atgtacgctc gtctcaaagg ttaagccatg catgtctaag tacatactaa aataaagtga 420 aaccgcaaaa ggctcagtat aacagctgta atttcttaga tcatattccc agtacttgga 480 taactgtgga aaatctagag ctaatacatg caaataatgc gagtatcgca agattctcgt 540 gcagttatta gacaaaccaa tcggtcttcg gactgtttta tatggtggaa ctctagataa 600 ctttgctgat cgtatggcct tgtgctgacg acagatcttt caaatgtctg ccctatcaac 660 tttgatgtta gtatagagga ctaacatggt tgcaacgggt aacggggaat cagggttcga 720 ttccggagag ggagcctgag aaacggctac cacatctaag gaaggcagca ggcgcgtaaa 780 ttacccactc ccggcacggg gaggtagtga cgaaaaataa caatatagaa ctagttaact 840 atgttctgta attggaatga atcaagttta aatcctttaa tgaggatcaa gtggagggca 900 agtctggtgc cagcagccgc ggtaattcca gctccactag cgtatgttaa aattgttgcg 960 gttaaaacgt tcgaagttga ttattgtcca gtgcgggtgt gacatcatca agaaatgatt 1020 gtcatcactt tccggttact tggactatat gactgaacac taaatgaata gcattgatgt 1080 ctttcactag actagatgtc tatttagttc ggcttacttt taccttgaac aaattaaagt 1140 gctcaaagca ggcttcatat gcttgaatag ttttgcatgg aataatggaa aaagacctcg 1200 gtcttatttg ttgcgtgagt tcgctcgcgt aatgattttc tattggtttg atacagatca 1260 agaggtaatg attaatagaa gcagttgggg gcattagtat tacggcgcga gaggtgaaat 1320 tcttagaccg tcgtaagact tactaaagcg aaagcatttg ccaaggatgt tttcattaat 1380 caagaacgaa agttagagga tcgaaggcga ttagataccg ccctagttct aactgtaaac 1440 gatgacagct agcgattggg tgtcactaca taatggtgca ctcagtagct ttccgggaaa 1500 ctaaagctag tttccggggg aagtatggtt gcaaagttga aacttaaagg aattgacgga 1560 agattacctc caggaatgga atttgcggct taatttgact caacacggga aaacttacca 1620 ggtccgaact caagtgggta tgacaatttg atagcgtttt cacgaatttg agggtagtgg 1680 tgcatggccg ttcttagttc gtggattgat ttgtctggtt aattccgata acgaacgaga 1740 ctcaattatt taactagata cctatcagct gttgagatgc tgaagtcatg tggtgtgtgt 1800 gtcgcaaggc attcatgaca tgtgatggag tctcacctga tagtttggct ttcgatttcg 1860 gtcgataagc tttatctgct taaatagaca aattgctttc agcaatatga aattgagcaa 1920 taacaggtcc gtgatgccct tagatgttct gggcggcacg cgagttacaa tgaagctgac 1980 aacgtgttac cttatccgag aggattggga aatcacttag ccagcttcct agttgggatt 2040 gtggactgaa aaagttcaca tgaaccagga actcctagta agtgtgagtc actagcttgc 2100 attgattacg accctgatct ttgtacacac cgcccgtcgc tattaccgac gaattattta 2160 gtgagatctc tggaggtaaa cattgtgatg cctcggtatc acgattgctt ttgccaaagt 2220 tgatcaaact tgatgatttg gaggaaata 2249 <210> 2 <211> 1433 <212> DNA <213> Chironomus riparius <400> 2 acgctcgaca aaaaaatcat tgtttgaagt ttgcaaaaat ttaaatttta aaaataattt 60 ttcaaattaa ttcagttttt taaaaaaaat tgacaataat ttcaagttta attaagaaaa 120 aaaaataaaa attaaaattt aaatatttgt gaattgtttt ttgaattttt gtaaagtttg 180 aaaattctga aaaaagtaaa aagttttaaa aaaataaatt aattttttga agttttaaaa 240 aatccttcta gactgaaatc aaaaatcaaa aaaggactca gaatgaaaat actcatagca 300 ttatcactcg cattctgtgc tgtcaatgga tttagtttct ttaatgcaca tgcagacccc 360 attgagattg caccattcaa cagttgtgct ggaattgccg atggtactcg acttcgaatc 420 gagagtaact gcaatgcatt cacactttgt gactctggat atggacttaa cttaacatgt 480 agattatcag agccagactt cgattggtgt actggaaaat gtgttaatga caagtcagtt 540 tgtacacaaa cgtcctgctc aacggatatg actacaacaa ctgttgctgg ggacacgaca 600 acaaccacgt caacgaccac aactgcatca acgaccactt ctacaacgcc agcagcagta 660 acaacaacaa caacaacatc aaaaacaacc actccattca atatcccaac agttccaact 720 gtatgcagtc ctgaatgtga tcctgccttg tgtgtagcac ctggttgtcc tgaacaaatg 780 gtctgcaaaa ttggaaatgt ttgtgaatgt gactcaacca taatgtgtcc aacggattta 840 gacacatgtt cattacccga aaattgcgga tgtgcatcaa attgtgagtg ctctgatcca 900 gacactaatc ttttatgcac atgcattgaa aaagacacat cagtctgcac attcgacaga 960 gaaacacaat gcgttgaaaa gtgtggatgc gattacagat gcgacagttc cgtttgtacc 1020 gaaagtacaa caaatcctgg atcttactta tgtactggat gtaatgaaga aaaatcatgt 1080 ccatttaaaa aggaagaatg ccttgctaaa tgcggctgta caaagaattg cgattgtgat 1140 cttactggaa gtacttgcaa ctgtttttcc acatgtcaat gtcctacaac accaccacca 1200 gatgtctgtc catttgtccc agatgattgt ttcacaaaat gtggttgtgc tgaacgttgt 1260 aactgtaatg gacctttctg catgtgtaat gttgatgcag tgtgtcctga aatttaaata 1320 aacttaacct taacatttta agttaattta ataactttaa aaaattttaa atgcttttta 1380 aaataaattt aaataaaatg aattgtttaa aacttaaaaa aaaaaaaaaa aaa 1433 <210> 3 <211> 1031 <212> DNA <213> Chironomus riparius <400> 3 gctcgtgtaa ttgaagtata atagtcaata ccttcgaata aagagtcgat ctcaatacta 60 gcttgagttg atgatgaaag agttctcttt gcacgttcgc atgctgttct caaacgacga 120 agtgctctct tatttgatgt taaatccttc ttatgcttac gcttgaattc ttgagcgaaa 180 tgattaacca aacgattatc aaaatcttca ccacctaaat gagtatctcc agcggttgat 240 tttacttcaa agattccatc atcaattgac aaaatggaaa catcgaaagt tccaccacca 300 agatcgaaaa tcaaaacatt acgttctcct acagctttct tatctaatcc ataagcaata 360 gcggctgcag ttggttcatt aataatacgt aaaacattta atccagagat agttcctgaa 420 tcttttgtag cttgacgttg tgaatcattg aaataggcgg gaaccgtaat tacagcatta 480 gtgacagtct tgcccaaata tgcttcagct gtttccttca tctttgtcaa aaccattgag 540 ctaatttctt ctgggaaaaa gttcttggct tcatccttgt acataacttg gatttttggt 600 tttccttcgg cactaataac ttcgaatggc caatgcttca tgtcagattg aactgctggg 660 tcatcgaatt tgcgtccaat caaacgcttg gcatcaaaaa tggtgtttgt tggattcata 720 gcaacttgat tcttggcagc atcaccaatt aatctttcag tgtcggtgaa tgcgacatac 780 gatggagttg tacgattacc ttgatcattg gcaataattt caacttttcc atgttggaaa 840 actccgacac atgaataggt tgttcctaaa tcgataccga ctgctggagc ttttgtagcc 900 atcttttatt ctttattcaa tgttttcgat tattaattaa cgtttttaat cactttaaag 960 tatttaattt aaggatttga gatctaatta aactatttta aactagcaca ctttagtctt 1020 gacgcactcg t 1031 <210> 4 <211> 725 <212> DNA <213> Chironomus riparius <400> 4 aaaataaagg tttagccaaa ggtggcaaaa aaggaaccaa aaagaagatc atcgatccat 60 tcactcgtaa ggattggtat gatatcaagg caccatccat gttcacaact cgtcaagtgg 120 gcaagacatt agtaaatcgt acgcaaggaa caaaaattgc aagcgaaggt ttaaaaggtc 180 gcgtgtttga agtttcatta gctgatttgc agaacgataa cgatgctgag agatctttcc 240 gcaaatttaa gcttattgct gaagatgtcc aaggaagaaa tgtgctcaca aacttccacg 300 gaatggattt aacaaccgat aaattgagat caatggtcaa gaaatggcaa acattgattg 360 aatgccacgt tgatgtcaag acaactgatg gatatttgtt acgtgtattc tgtattggat 420 tcacagccaa ggacagttca tctcatcgta agacatgcta tgcccaaacc tctcaaatca 480 aaacaatccg caagaagatg acagatatca tcaatcgtga tatcgccagc tctgatttga 540 aggaagttgt taacaaattg ttgcctgatt caatcgccaa ggatatcgag aaggcatgcc 600 atggtgttta tccactccat gatgtcttta tccgtaaagt aaaggtattg aagaagcctc 660 gtttcgatct ttcgaaatta cttgaattgc acggagatgg aggcaagtca actgaaccaa 720 cagcc 725 <210> 5 <211> 1793 <212> DNA <213> Chironomus riparius <400> 5 acgctcgaca gctgattcaa atatcttacg agtaagacgt cgaaggaaaa agatttaagt 60 tgcagaaagt tctagaaatt tctcgaagga actgacggag aaagtgaaaa caacagtgat 120 aaatagtcaa tagtatctta aggatttttt acaataaagt ttaagtgaat taaattaaga 180 attatatatg atgacaacat cattgacaag tgccgaaaag aagtaaaagt tagtctttga 240 cgcagtgtag aatcataatt ttggtcttac aaaagaaaaa tatattttga gtataaaaag 300 attataatca gtgctagatt acaatgcatc caatagagag cagtggaacc tcatccgttc 360 cagcattttt agcaaagtta tggcgattag tcgaggagca agagacgaat tccttaattt 420 cttggtcaca ggatggcaag agcttcatca ttcagaacca agcgaaattc gccaaggatc 480 tccttccatt aaactataaa cacaataaca tggcgagctt tattagacag cttaatatgt 540 atggctttca caagattaca tctattgata atggtggact aaagtttgat aaagatgaga 600 tggaattttc tcatccatat ttccaaaaag gtcatccata cttgttagag catattaaga 660 gaaagatcgc caatccaaaa cataatgaag cagaaaaaac ggcagcaaca aaagctgagc 720 atttcaatag agtattacat gaagttaaga atatgcgtgg acgacaggat tctttagata 780 cacgtttcag tgctatgaaa caggagaatg aagcattatg gcgtgaagtt gccattttac 840 gacaaaaaca catgaaacaa caacagattg tcaacaaact cattcaattc ttggtcacgt 900 tagttcaacc acaaagatcc ggtcttggaa atagtctcgg aaagagacgc ttccaattga 960 tgattaacga cgcaccacaa tcaaaagtca aaaagtcaaa tcaaacgggc ccaattatta 1020 gcgagttact agaacaatgt gattccgata tggatacaga cgaattcaat ttcacgaatg 1080 tccaatcgcc tggatcatca tcaagcaagg attttgataa tagtgcggga tcttcagaag 1140 gattaaactt tgatgatatt gatgaaattg atacaaacag tatggctatt tataacggcg 1200 ttcaagctgt taaaaaggat agacgctcaa aaaccaataa ttttacaggt aaattggaac 1260 caacgaggtc gacaaatcaa caaaagcatt tcaaaatcga aaataatgag ccagttattg 1320 tctcaccgaa tccatcacca ggaaagagct tactactatc aatggctgaa aattccaata 1380 acgaaactga tcaaaatatc atgattgata tgctaaacga tgaagaagtt gagttatctg 1440 gtgataaatt gaatggcaac ggaaatggtg caagctcaac tatcacgagc ctggatcctt 1500 tggttgcaca aaacactctc gatttcgatc atacatctca agatgtacac attctcggaa 1560 ataacgatga cgacgacgac atttttgaat ttgctgaaaa gaaggacgaa acagttgatc 1620 cgaatgcctt aaaagttgtt aaatttaatc ctccacatgt tgaagatatc ataaatctgc 1680 aatcgagtaa cgattataca acacatatag ataatgtaca aaacgatttg aattctttga 1740 aggacttatt gagcaatgat gcttatcagc ttgatgcaaa tcaattatta ggg 1793 <210> 6 <211> 552 <212> DNA <213> Chironomus riparius <400> 6 aaataaaaaa tgaaaagaaa aaaatcgtac aagagttgcg ttatcaacag caaaacctcc 60 tcataatcat tgaacaagat cagttcctat ataagggata gcagataaaa ggttagtaat 120 tactgtagcc cctcaaaaag atatttgtcc tcatggtaat acataaccta taaaagctgt 180 tcctatagtt aagaaaaata gaattactcc tacagttcat gtgtataaat atttatatga 240 tccataataa attcctcgtc caacatgaag ataaatacaa ataaaaaaaa atgatgcacc 300 attagcatga agtgtacgta aaagtcatcc gtaattaaca tcacgacaga tatgatttac 360 agaattgaag gctattgtgg tatcagctgt atagtgtata gagaggaaaa ttccagtagc 420 aatttggata attaaacaaa gtcctagaag tgaaccgaag tttcatcatc tagaaatatt 480 agagggagct ggtagatcaa ctaatgcatt atttataatt ttaaataagg gatgggtttt 540 tcacgcactc gt 552 <210> 7 <211> 594 <212> DNA <213> Chironomus riparius <400> 7 tttctggacg atacttttcc aaaaccttga acaattgaat tgcagtttgc ttgtcaacag 60 cttgagagaa ttgatgaatt ggtggaggaa ccttcaagcg cttttgtaaa attgccttgt 120 ggcgttggat acgaatgtat ttgggccatc ttacgaatct tgagagatca cgtttaggct 180 gtacattctg accgattccg tagttcttcg ggcgtttttc gaaaagtgga tttgttgcag 240 tcttcttagc gactgctgat tttgataatg gtgctgcagc aactttcttg ccaacaacct 300 tctttttgct tactggctga aattagcaaa aaacattggt cattagcatt ataataagcc 360 agtattgatt caaatcttaa ttgaatcaac tttaattcat gtccaatttt caaaacacat 420 tgttttaggt tagacgaaat aatatttttt cataaaatca ctgattttcg catttctttg 480 ggaaaacttc atgatcgttg attaattaat gcacttatac gtcttttgga atacaaacat 540 acctttttag taaccatttt ccttattttt ccacgtgatt ttcgggaatc gaag 594 <210> 8 <211> 3109 <212> DNA <213> Chironomus riparius <400> 8 aaggttctaa tcaagcatca aagataacca gtccaagagt tgttggtagc ttaaatcaag 60 tggaataccg aaaattgttc tcaaagaata caaaggcaat cgtttgggga atgcaaacaa 120 gagctgttca gtcaatgttg gattttgact tcatttgtcg acgagaggaa gcatcagttg 180 ctgctatggt ctatccattc actggatatc atcgtcaaaa gttttattgg ggacataagg 240 aagtcttgat tccagtttac agtaaaatgg ctgacgcact gaacaagcac aaggatgttg 300 atgtacttgt caattttgca tcacttcgtt ctgcttatga atcatcactt gaagttgtca 360 atgactttcc acaaattaga acaattgcta ttattgctga aggtattcca gaaaatatga 420 ctcgtaaatt aaactcagca gcacatgaaa agaaagtatc aattattgga ccagctactg 480 tcggtggagt taaacctggc tgctttaaaa ttggaaacac tggtggaatg ctcgataaca 540 tcttacactc aaaattatat cgtcctggca gcgttgctta cgtttcacgt tccggtggaa 600 tgtcaaatga gctcaacaat attattagca actcaacaga tggtgtattg gaaggtgttg 660 ccataggagg agatcgatat ccaggaacag tttttatgga tcatattatg cgatatcaac 720 atgacgatga tgcaaaattg attgttgttc ttggagaagt cggtggcgtt gaggaatatg 780 aaatttgtga agcattaaag gacggaagaa ttacaaaacc attgatcgca tggtgtattg 840 gaacttgtgc tggaatgttt acatcggaag ttcaatttgg acatgccggt tcttgtgctc 900 aatctgatct ggaaacagca ttatcaaaga acaaggcatt agcggaagct ggtgctcatg 960 tccctgaatc atttgataca cttggagatc tgattgctca tgtttatgaa gatatggtac 1020 acagtggaca gattgtgcct gctgaagaag ttccccctcc taccgttcca atggattaca 1080 attgggcaag agaattaggt ttaattcgta aaccagcctc attcatgacc tcaatttgtg 1140 acgagcgtgg acaagaattg ctatatgcag gaatgccaat ctctgaagtt ctcacaaaag 1200 acgtcggtat tggtggagtt gtttctcttt tgtggtttca acgctgctta cctccatacg 1260 tctgtaaatt ctttgaaatg tgtctaatgg ttactgctga tcacggacca gctgtcagcg 1320 gtgctcacaa tacaatcgtt tgtgctcgtg ctggaaaaga tctggtttcc tcattagttt 1380 caggattatt gactattggt gatcgtttcg gcggtgcact tgatggagct gcaaaacaat 1440 tctcagaagc atacgattcg aatatgcatc caatggactt tgttaattcg atgcgcaaaa 1500 agggacaact tattatggga attggacatc gtgtaaaatc aatcaacaat ccagatattc 1560 gtgttaaaat tattaaagat tttgtactcg aaaacttccc ttcaaagcca ttattggaat 1620 atgcacttga agttgaaaag atcacgacaa gcaaaaaacc aaatttaatt ttaaatgttg 1680 acggagttat tgctacgtgc tttgttgaca tgctcagaaa ttgtggtgca ttttcaagcg 1740 aggaagcaca agaatacata aacattggag ctataaactc attgttcgtg ttgggacgaa 1800 gcatcggatt tattggtcac ttcatggatc agaagcgctt gaagcaagga ctttacagac 1860 atccatggga tgacatctcg tatgttctgc ctgaacaata caacgtaaac caataaacgc 1920 aacatcccat gatctaaaaa tgatatccta cattttaacg ttagaaaatc gcttattatc 1980 tgaatgtgtt cctggctatt ttattgtaat gatggaactc attcgactat gatcgttata 2040 aaaaaaaata ttagtaaatt ttaatcttga ttcttggtgc tataaatttt gtcatttgga 2100 accaataatt atggatttta tagcaatgaa aatcaaacaa aacagaatat ataatgcaat 2160 gcatttaaaa tgaaacaaaa ccgcactgaa atcatggata agcacctgat aaatgttatg 2220 taggaaatga aatgtttttt gcactaaaac ttgtagaaag aaattttttg cacaaaaaag 2280 aaaatcataa aaataataat aattaaacga tgataatcag agttgggtga gcttgactgt 2340 taacagctct gtattaagca taaagtaatt tcttatttaa gtaaatggtt tattatatta 2400 atttcttttt gaaggttgat ttacattcct aattggaaaa aaaaagaatt aaaaaaatat 2460 tattaatcat agtttcaagc attagttttc attattttcg tcatttttgg gttctttatt 2520 gttatttttt tacttctttt tttgttttac aacattaatt tttaatttaa tgacatgaaa 2580 aataagtttc aagataaata aaaattacat ttatgttata gaaattcgaa aatacaccaa 2640 caatcttatc taaattccaa aaactaaggc tttccacgtg tcaacatttt aatctcttga 2700 ttcttgtcac caaaacttct ttgttttact agacacttgt gtctttgtca aatattttcc 2760 tagtattgtg attcaacaga aatataatca gcttcaagag atcaataagt taatccattc 2820 agttaagatt atattaagca caactagttg tcagattaat gttaatactt gaattattaa 2880 tcaattatgc gtgcctaaga agcttttaag tagtgaattc tttacaaatc acagggacgg 2940 gattttggta tatctggata aacccacata taattttctg atgactttta ttgtgagtgc 3000 aatgggtata tcctaattat actcaaatta atctagtccc agttctaaaa cttaacaaaa 3060 aaatttccat tttgccctaa taaatatgaa gtcagtcctt taacgtcgc 3109 <210> 9 <211> 2512 <212> DNA <213> Chironomus riparius <400> 9 acgagtgcgt cttgaataaa gtcgtaataa cttaacactg cttaatattt ttaaaataat 60 agtttgtttg gaaactttgt atagttttta ctcatttcaa aatgtcgaaa aacttaagta 120 ttgttaataa ggatttaaaa gaagaacgtg acaaaattaa ttttaatgtt gaagaattta 180 ccaattggtt ttatggtgga gctgaaaaag taaaggaaaa gaaatatatt gaaaattatt 240 tcttatctga taaggatcta aatttagaac ttgatacgag ttatttgagc tataaggaca 300 aatatgaaga agctgtcaga aggagtattg ttgtttacaa aaagctttat aaacttcata 360 acgaaatggg tggaacacat gaagattttg taaaaattat ggaaaatctt cgctttacaa 420 tgggagcaat aaatcctgaa ggaaatccgt tcgttttgca tttttcaatg tttgcactta 480 ctatcatgaa tatgactaat gatgaacaaa aagcagaatg gttacctcgt atttttaact 540 gtaatattct tggaacatac gcacaaacgg agcttggaca tggaacttat attcaaagat 600 tggaaacaac ggccacatat gatccgaaaa caaaagaatt tactttaaat tcaccaacat 660 taacatcgta caaatggtgg ccaggaaatc ttggacatac agttgatcat gctattgtta 720 tggcacaatt gtattcacaa gggaaatgtc atggaataca accatttatt gtacaattga 780 gagattcaga aacacataaa cctttacctg gaattgatat tggagatatt ggtaataaag 840 tcggtttcca aacggtaaat aatggatttt taggtttcaa gaacgtaaaa gttccttata 900 aaaacatgct gatgaaaaat tcaaagttgc ttgaagatgg aactttcatt aaaccaaaga 960 atgctaaact taattatgga actatggtta ttattcgagt tcacatgata atagatgctg 1020 cttgtcgtat tgccaaagcc acaacaatag ctttaagata ttctttggta cgacgccaaa 1080 gccctattaa tccaaatgaa atagaaccaa aaattattga acatattaca cagcaaaata 1140 agatttttcc aggaatcgcc aaggctgttg ttttcaaatg tatggctgac tatttaacaa 1200 agttgcatct ttcaataaca caagaaggaa atacaggcaa cttggctaga atgccagagt 1260 tacatgcact ttcatgctgt ttaaaagctg tttgtactaa tgaagatgca aaaactgtag 1320 aagtttgtcg actttcatgt ggaggacatg gattcttacg ttcagctgga ttagatgatg 1380 tgtatagaaa ttcaactgct gcacagacgt atgaaggcga aaatacagtt atgttattac 1440 aagcagctcg atttttaatc aaatcgttta atgaggctag aaacggtgga tccttgacag 1500 aaggagtaaa atatttattt gattttattg gacgaaacgg aaagcgtgaa atttttgata 1560 attcaattaa tggaattttg agagccattc aagctgctgc tgctggtaga acatatattg 1620 catggaaaca cattgaagat gacaagcgtc ataattcaat tgaagttgca actaatttag 1680 caggtgtgca cttaacaaaa gcagcagaac ttcatggtta cgtctgtctt ttgcaagttg 1740 gaattgaaga aattgaaaag ataattcgta atgtatctcc agctcttggg ttggttttca 1800 aaaatatctt agaattattt gcagttgatt taatccttaa caaaattggc gatgccattc 1860 agcatgttga tatatctaac gaagatattg aaaaattaga aaaacagatg gagactttat 1920 tcaaattctt taggacatca gctattggag ttgttgacgg ctttgatttt tctgatccta 1980 tcattgattc aactttgggt tcttacgatg gaaacgtata tgaaaactta attaatgccg 2040 ctagaaaatc gccactaaat caagaggatg ttaatagaac atttcatctt tatataaaac 2100 ctttcatgaa atctaacttg taattaaatt ttaaacaggt tttattttct atttatatgt 2160 ctgtaatttt aacaaatttt gaattgattt taacaaaaca tgttgagtca aaaaatttta 2220 tattttatat ttgtaattgt taagcaatgg ttgtggaatt tatattaaaa gtctattaaa 2280 tgacaaatgt taaattttga aaatatcgga atccaaaaag gagatgttat ttattttgtt 2340 cttcatataa accctaacga tccaaataga aaattatatt caatcggggt ttaatatgct 2400 gtccgtaatt tatttcacga tatcatatcc taaccgacaa gtgagtatac tcatggtacc 2460 gtaacatact ctatagaata tgtatgttca gaatatactc ttgagtatgt ta 2512 <210> 10 <211> 693 <212> DNA <213> Chironomus riparius <400> 10 acgctcgaca tgattggagc aaaaattatc aaaagtcaag gcgctgagcc tgatgaattc 60 gagaaatcta tcggacaagc ccttttggag cttgaaatga actccgattt gaagcaacaa 120 cttcgtgaac tctacattac tcgcgctaga gaagtcgagt tcggaaccaa gaaggcaatc 180 gtcatttacg ttccagttcc aaaacaaaag gcttttcaaa aggttcaaac tcgtttggtg 240 agagaattag agaagaagtt ttcaggacgt catgtagttt tctgtgctga gcgtaaaatc 300 ttacctaagc cacaaagagg acgtccagat ccaaacaaac aaaagcgtcc aagatcaaga 360 accttgactg ctgtatatga taacattttg gaagaccttg ttttcccagc tgagattgtt 420 ggcaagcgca tccgagtgaa attagacgga tcacagctca ttaaagtaca tttggataag 480 aaccaacaaa ccacaattga acataaagtt gatacattca cagcagtgta taagaagttg 540 accggccgtg atgtcacatt tgaattccca gaaccttatt tgtaagaagc ttctttaaac 600 agtttgagct ccgagaaaca agacaccctg aaaactaatg tcttgttaca cttgatgtga 660 aataaataaa agaatattat taaacatctt aaa 693 <210> 11 <211> 3561 <212> DNA <213> Chironomus riparius <400> 11 cgtttgttaa aaaaattttt tttaaacttt ttgttcttgt attttctata aaaattagta 60 tttttttttt cattcaaata taaaaaatag acttttattg cattttcttt atctcatcga 120 aaatttttta taaaaagaat cgttttttat gcaaaaaaat gtccaagtga ggttaaattc 180 ctagaaatat gatccacaga acgaaagtta tcttcatgga tgatatttaa aggatttcat 240 gtccacacaa gtttgcaata tgctcacgtg atttcataaa agaattagta aggatatgct 300 taaaatcaaa tttaataaat acacactatc tcataaatcc tatttttttc tgacgatgtt 360 aatcaacaaa ttcgttataa ctcattaaag taaagatcta tccatcttgt aaaactaaat 420 cttaacaagg aatcaaagtt tttatttagc acttaagttg taagttcaat atgcattatg 480 cattttttta attgcaaaaa tttaacataa atttttgaca cacacattaa tgtaccctta 540 tttttggtat gaatggcgta tgcttgaatg aaatcacatg attatattga ctcccgaatc 600 cgggaattga tgttcacaat tatatataaa aaaaagtcat ctgagaactt ttgttttgtg 660 tttttttaag taaatttctc tcattttata ggcaataatt tttttttgga agataaaatt 720 ttactcaata caaaaattat atattttggt gccacagatg ttgtgcagtt taaggatttg 780 taacaaacaa ggagttttaa gaaagcgttt cacgtaaatc acgaaaaaag tttcgcacaa 840 tctgcggaca ccttatgact aaaattcttt cattatggtc gatccaagga cgctgcttgg 900 atcttatcac gatcattatt gaagcacaaa gtgagtaaaa ttctatcttt cttattgttt 960 tcaaaacgtt tataaacaaa ttttgggaat ttttttctcg aaactgattt atgagccttt 1020 tttctgtgga atttcttaag gaatttggaa atttttaagt gagtttcaac tttaaatact 1080 ttaagttcga ggttgactct cagagatctt ttagagtgca gtttttaact actaggcaag 1140 atgactaagt ttatccgata ggttaggtct gtgaaatagg tatcaatgta atagatatta 1200 gaggttttag cttagtccag gtatacacaa agacaacagc attttcagag atttggagtc 1260 aaccattcct ctgttattga acatctacaa tcacaaacca acctctggat tgatatggcc 1320 aacaatattt tcatcactac ttttaattct tgcctaattt ttggattttc caataaagtt 1380 caaaaatatt ccaaacaatt ttaaaaattc gccacaaaaa aaagtcccac aaatcagttt 1440 cggtatggaa agcaggaaat aaaaatttta aggtttcttt caaagttcaa aaaactaaaa 1500 attagaagta aaaaattaaa ttaattttat aaagtaaaaa aaaagaatct actaaaggta 1560 aggttgttgt tttttgtaga agattagtag atctaacagc attttaatgg acacattcca 1620 agatgtttct catcaaaagc tagtcaaatc actaaatctc ctttacactg aggttttgtg 1680 ttttttttct tttaaatagt taataatttt tttgctgtat tattattaat attatttaaa 1740 tgtttttttt tgtatttagc gcattctttg cttaaggaaa tgcgattgat ttcttataaa 1800 ttatgataat agaaaaaatc tgttcaacac aacttcccgt ccaattcaat ccaaattgtt 1860 tttttctgtt cattttcagg gaatcaaaaa aattattaaa gttcattaga tagtaaatag 1920 aactacataa taatagtaat aataatatta gaaactgcat ttaaaaggat ttcttgttga 1980 tgttctgttt ctgttccata atttaagatt attttccagg catagacgat taattactca 2040 atgaaggatg tatagtttga tagtagaaag agatagtagt agatttaaaa aaaattgtag 2100 tttattaacc acttggcgtt ggacatacaa tgtatagttg tcgtgctgag cgacgacgtg 2160 attgtaatgg acttgcaaca tcatcatcat tgacgcattt attaatgata ttattcatac 2220 cgccaccact tttatttgta tcactaagga cattgtccaa aattgtctga atatcctcat 2280 ctgatgatgg ctccgatggt gttgttggat gggatccgcg tgatgatgac tcactgatgt 2340 aatggatgtc atcctcatcg ctactactta attgacgatt tgtcggtggt ggcaaatcag 2400 gcattaagaa tgaatcattt gaactgctat gatgtggatg gtgttgttgc ttcttttgtt 2460 gttgtgggat tggttgcggt tgtgtctttg ctgatggttg ataaatgttg gatcgctcct 2520 tgttcaaata atttagttgc atggaaaatt gttgtaaaat aactcggttc acagccattg 2580 ccgactcagc gaattgagat aatttacgag cagattgatt gcttcgaata atccctggag 2640 ttttcaacgc acgatggcgc tttccgttgt cacaattctg atcagcgact cttatccagg 2700 gatgggttag tacagcctca gctgatagtc gcttcgatgc ttccttgacc aataaattac 2760 gaatcaaatc cttagcttca ttactgacgt ctcgccattc ggtatcaggg aaacaaaaac 2820 gtccttcacg aatagactca aataaaagtt cttgacattt ctgacagttt tcacctcgat 2880 tccaaccaca ttcttgtcca caattgccag aaaatggtgg atagccagat agcaaaatat 2940 aagcaataac tcccattgac cacaaatcac atctcttatc gtagctgctc tcacgttcgc 3000 caacaaatag atcaaccact tcaggtgcca taaattcagc acttccaacc ggtgtcaata 3060 gttgcggtgt ggcaaccgat tcggaaagat cagtcgtaaa attaattccc gagccgagat 3120 caaaatcaca tattttaatg ggacaaattg tgtcgggatt gacacataga atgttttcag 3180 gtttaagatc acgatgcgca atgcctttct tatgcaaaaa gttcaatcct gaggcaatct 3240 ctttgattat ttgtgctgca tcatgctcag aaaagcaaat gttttcttga atgcgcgtca 3300 atagaggccc tccattgatc ttttcaaata ctaagtagaa cttctcctta tcctcaaaga 3360 attctattaa ttgaagaatg tttggatgtc cttggcaatg atggaatgtc tccacctctc 3420 gaaagactcg agctcgtgca tgacctggta tcttatcaat aattttaacg gcacactcta 3480 cttcggtgaa tatatttata catgtttgta ctgaggcata ggcaccttct cccaatattt 3540 cgcctgtcaa tttatacagt t 3561 <210> 12 <211> 1539 <212> DNA <213> Chironomus riparius <400> 12 cacctgtttt gctgattgag actaaaaagt acgtctgggg ctaaaaatca aattaaaatc 60 agcataaaat gtacgaagac ttgatgaacc gtgccaatgc ggcatttcaa agtggcaaga 120 caaaggatgt tgattttagg aagacacagt tgcgggcgct gttaagaatg tatgaagaaa 180 ataaggaacc aatggcaaga gcacttgctg ctgacttgag aaaaccaaag caagagtcat 240 acgtgtgtga aattgatttt ctcattaatg accttaaaaa tatgttgaat gttgtggatg 300 actgggttaa gcctgaattg cctaaaaaag gtcttgtcaa tatcatggac caagttctag 360 tctatccaga cccattgggt gttgtgttga tcatgggtgc ctggaattat ccaattcaat 420 tgccattgct tcctcttgct gctgccatag ctgctggaaa tgttgccatt gttaaaccaa 480 gtgaaattgc tgtgaattgt gcaaatatta ttgtggaaat gatgcccaag tatttggata 540 atgaatgtta ccacgtcgta tgtggaggaa tcccagaaac cacagagctc cttaaataca 600 aattcgacta cattttctac acgggatcag gtcgtgttgg aaaaatcatt tatgaggcag 660 ctgcaaagca tttaactcca gtcacattgg aattgggtgg aaagtcacct tgttatgttg 720 acaacagcgt tgacatctcg atagcagcaa gaagaatcat gtggggaaaa ttcatgaatg 780 ccggtcagac atgcgttgca cctgattata ttttatgtac caaagaagtc caagaaaagt 840 ttgtaaacga agccaaaatt attgttaagg aatggtatgg agatgatgtc aagaagagtc 900 ctgacttttg tagaatcatc aatcaggcta atttccaacg catcacaaag cttatggaaa 960 ccgggaaaat agctctcgga ggcaaaaccg acccagaaga taggttcatt gagccaacag 1020 tcctagtaga cgttaatgga gacgatttag tcatgcaaga tgaaatcttc ggaccaatcc 1080 ttccaattgt aaatgtcaat aatgcatttg aagctattaa attcatcaat tccaaagaca 1140 agccactcgc actctacata ttttcaaacc gcaaagcaga tagagatgct atcatcggaa 1200 acacatcatc cggtggagtt tgtgtgaatg atacaattat gcatatggct atagacacag 1260 tgccatttgg aggagttgga ccaagtggaa tgggaggata tcatggaaaa ttctcttttg 1320 atacgtttgc acataagaag ccttgcctag tcaagaagat tgacaaaatt ggagagaagc 1380 tagctgatgc ccgatacccg ccatacacag atggaaagat caaatacttg agcttcctca 1440 caaagaaccg caaagtgcca tcaattccat acttttccca catggtcctc ttcggtcttg 1500 gtattgcatc ggctctcctt gtccaacaag ccttgcctg 1539 <210> 13 <211> 1036 <212> DNA <213> Chironomus riparius <400> 13 ttttaaattc atattttatt taaaaatttt cttatttttt gaaaactata atcttgtcag 60 ataaagtgat aaaaagtaaa agggttaata gtgaaaaaat cgtggacctg tgtcaagtaa 120 ctttctatgt agactcaact acaaataaaa ctatgcaaat ttgcacaacc agttcttgtt 180 cttgttgttt aaagcctcct gaaggtctct tacttggtct ttcagcttct cgttttcttc 240 gttgagcagt ctcagtttat caccaaatac atcatgacca tcacttttta tgaaggtagt 300 gaatttcgaa acatccgtta aaattacatg ttcaggtctt ttacaatcat tctgtatatt 360 ctttagagat gttgaaacaa ttccacgtaa ataaaagttt ccatctttaa gaacaattaa 420 tccacttcca atatcgtctt cacatgatcc agtaccattc tcatatacac cacaatatgt 480 tctagaaccc aaaattgact caagattctt atttgttgaa caagtttgat gtttaatgat 540 ataaactttt gaagtcattc ctgaatcgag atcaccaatt ttattaaatc caataatttt 600 tcccggcaag ttatcaaact tttcagcatc agattcaatc aaacaaatcg gttggataaa 660 gtcgtcaaac tgaatttctt cagttaactc caaaactgca atgtctgcct catatgaaat 720 ttctttttga ttccaatccg aatgaatatt gattgatttt acagacacag aaattctgtt 780 catttcagtt atgtctagat tgtgtccacc taatataact ttgatgtcag aaggcttatg 840 attaatgttt gctgcgatgt caatgaaaca ttgagccgct gttacaatcg ttttatttga 900 aaccagagta cctccgcaga catattttcc ttctgctgta accaaggctg cattccaagg 960 atatgatcct tgttcgattt gtgttcctcc aagaatagat tcctttccaa ttttatgggt 1020 accacaggtt aaattt 1036 <210> 14 <211> 1801 <212> DNA <213> Chironomus riparius <400> 14 ttaaaaattt tattttataa ataatttaaa taacgacttg aaaatgtgag aaaaactgta 60 tatctgctat gaaaaatcaa aaagagatgt cacgaaagaa attcatttcc ctttttttgt 120 agattaatgt gtagtagttg attgctattt actattatta ttcttgatgt tgtatgcatt 180 ttgcagtgag atgtagtact gcttaatttc ttcatttaca taatgaacat tggcaactat 240 taattatagt tgcaatgact atgaaaaatt atggtatatt caattcatac ttatcgcatg 300 actatgtgcg ataaattgaa tttcaaaata aaatataatg tttatttttc ttcgttctat 360 ttgtctttta attaaaaatt aattaacgtc taaattcctt aagttgacgt aaattcatcc 420 atgcatctta aagcaaagat tgtaattgag taatataaat aatttgtgta ttttttgaat 480 gatacgagag agaaaagtta aaagaaaaag ccaagagcca acaacacacg aaggatggca 540 gtttgtgtgc attagtaaaa taaaattgat tgaatttgta agctgatttc ttaatttaga 600 agcattttct gtggacaatg cctgggccag attcgtcata ttcttgcttg ctgatccaca 660 tttgttggaa ggttgagagt gatgccaaga ttgaacctcc gatccagacg gagtatttac 720 gttctggtgg agcaatgatc ttaatcttga tggttgatgg agccaaggca gtaatttctt 780 tttgcatacg atcagcaata cctggataca ttgtggtacc tccagaaaga acagtgttgg 840 catacaagtc cttacggata tcaacatcac acttcatgat tgattgatag acagtctcgt 900 ggataccaca tgattccata cccaagaatg atggttggaa aagagcttct ggacaacgga 960 aacgttcgtt accgattgtg ataacttgtc catcaggcaa ttcatatgac ttctcgagtg 1020 aagttgaagc agcagctgtt gccatttctt gttcaaaatc caaggcaaca tagcacaact 1080 tttccttaat atcacgaacg atttcacgtt cagctgtggt tgtgaatgag tatccacgtt 1140 cagtaaggat cttcattaaa tagtcagtca agtcacgacc agccaagtca agacggagga 1200 tggcatgtgg aagagcataa ccttcataaa ttggaacagt gtgtgaaaca ccatctccag 1260 agtccaaaac aatacctgtg gtacgaccgg aagcgtagag tgaaagaaca gcttggattg 1320 caacatacat agctggtgag ttgaatgttt caaacatgat ttgtgtcatc ttctcgcgat 1380 tggcttttgg attcaatgga gcttctgtga gtaaaactgg atgttcttct ggtgcaacac 1440 ggagttcatt gtagaaagtg tgatgccaga tctttttcca tatcatccca gttggtaatg 1500 atgccgtgtt caattgggta tttcaatgtg aggataccac gtttgctttg tgcttcatca 1560 ccgacatatg aatccttttg acccatacca accatcacac cttgatgacg tggacgtcca 1620 acaattgatg ggaagacagc acgtggtgca tcatctccag caaaaccggc cttgcacatt 1680 cctgatccat tgtcaacaac gagtgctcca gcgtcttcgt cacacatttt gaattaattt 1740 aaagttatta atcagcaaga agaattgaaa tcgcgaattg taaatgccac aaagcgcaaa 1800 a 1801 <210> 15 <211> 2124 <212> DNA <213> Chironomus riparius <400> 15 acgctcgaca tttgaagtga gctcgtattt tttcttgtat caaaaattta aatttgatta 60 aaaccatcaa catgagtttg tataatttta agaaaattat ggtggttccg ccagcaaaat 120 cgttcatcga tattatgctg tcaagaacac aaagaaagac tccaacagta gtacataagc 180 actacaaaat atcaagaatt cgagcattct acatgagaaa agtgaaatat acacagcaga 240 attttcacga tcgcctttct caaatcattc aagattttcc aaaattagat gatgtccatc 300 cattttatgc tgatttaatg aatgttttgt atgacaaaga tcattacaaa cttgcactgg 360 gtcaactaaa tatggctcgc catttaattg ataatgttgc aaaagaatat gttcgtctat 420 tgaaatatgg tgattctcta tatcgttgta aacaactgaa gaaagcagca ctcggtagaa 480 tggcaacaat caccaagaga caagcgtcta atttgactta tctggagcaa gtgagacaac 540 atttatcaag attgcccacg atagatccat atacacgatc tattatattg actggatttc 600 ctaacgttgg taaatcttcc tttatgaata aaatcactcg agcagaagta gaagttcaac 660 catatgcttt tacaacaaag agtttgtttg ttggacatac tgattataag tatcttcgtt 720 ggcaaattat tgatacacca ggaatcttgg atcatccatt agaagataga aatgttattg 780 aaatgcaagc aattacagca ttggcacatc ttagagcttg tgtgctgtac ataatggata 840 tatctgaaca gtgtggtcat acattagaag agcaaattca tctttatgaa tcaattaagc 900 cactttttgc aaacaaacca cttatgattg ttattaataa agttgatata attaagcttg 960 aagaactgga tgaaacaaga aaaaatgtac tcgcaggagt tcttaatgac gaaaatgttc 1020 cagtcattca gatgagtaca gtcaacgaag aaggagtaat gcaagttaag attgaagcat 1080 gtgaaagact tttaggcttc cgtgtagatc acaaattaaa gacgaaaaag gttgaaaata 1140 ttttaaatcg tcttcatgtt gcaatgccag aaaaaagaga tgaaaaggaa cgtccaacat 1200 ttattcctga ttgtgtatta cagaagcgtg aagtagaaaa tgttataaaa caaaagagaa 1260 aattagagaa agatatcgaa gaagaaatgg gtgatgatta tattttggat ttgaagaaac 1320 attatgttac aataccagaa gaagagcgtt atgatataat tccagaattt ttggaaggac 1380 ataatatcgc tgattacatt gatccagata tatttgagaa attggaagaa ttagaacgtg 1440 aagaaggcct acgactggat agtggatact atgagccacc aaaattgaac attgatgaaa 1500 cattgcaaga gattcgagat atggcacgac aaattagaac aaaacgattt acattgcgcg 1560 ataataaacg cttagcaccg cgtgctgata aacctaagat tcctcgacac aaattacctc 1620 acgttcgtga tcgaaaggta gacaaattga agaatacaat ggaggaactt ggagttgata 1680 tgagtacaaa ggaagattct aatttcaaca agagcaaaat tcatgtacgt cgtagtttag 1740 tacctgctat tggtggaaaa gctgaaaaga gaaaggataa ggaatcgtcg gcaattgtca 1800 aatctacagg tcaaacattg aaacgaaaag caccaaaaca tgaacttgga ttgacaaatg 1860 ttgttgtaaa gaagaaagct caaattatgg ctaagcatga catcgctaaa aaattaaaga 1920 aaatgtgtct caaaggtgaa gctgacagat ttattggtga tttgaaaccc aaacatctct 1980 tcagtggaaa acgaggaaat ggaaagacag atcgacgata aacttatatt ttttgaaata 2040 atattttact cttaaacatt ttattatcca attgaaattt caagaaacaa taaacttcat 2100 gaaatattta caaactcaat aaat 2124 <210> 16 <211> 2223 <212> DNA <213> Chironomus riparius <400> 16 ttgaattgca aagagtcaag atgagacttt gtgtgttgtt tgtcgttctt atcactacgg 60 tgactgcacg ttatatgaca aatgaagtta aatatgcgga ttcggatttt atggtgaaac 120 aaaaagcaat ttttgaaatt ttcgcaaaca tttggcagcc agaaattcac aattcgtatt 180 atgatgaagc aaagaatttc aattatttga cgatcaaaga caaaattaca aatgaacatg 240 cctttgaatg ctttagcaac tgttatgaaa aaggtttcat tggaatggaa gatattttct 300 caccactaca aaaagaacat aatcatcaaa tgcttgccgt cttcaaaatg ttgtactatg 360 caaaagattg ggacgcattc tacaatttta tggtttgggc tcgctttaac ataaatcccg 420 gaatgtttat tcaagctgtc accatggctg tacttcatcg tgatgatttt gctggttata 480 ttttaccagc aatttacgaa ataagtccat attatttttt caacagttat gttattacaa 540 aagcacgccg tatcgaaatg atgggatacg agagcatgga gaaagtcggt agcgttcata 600 catacacaat tccaatgaat tatacgaatt attatattaa aacaaatcct gaatcaaaat 660 tggcctattt tatggaagac atgggtctaa attcttatta ttactattgg aatatggatt 720 attatggata tcttggagga gatcagtatg atttgaagaa ggatagacgt ggtgaatttt 780 atatgtacca aatacgtcaa attctcgctc gatattattt agaacggttg tcagtagggc 840 ttggtgagat tcctgaaatc aacttttggt caacaataaa tactggctac acttcaaact 900 tgatgtttta taatggcgtc aattttccaa ctagaacaaa ttattatatg atgcatctta 960 acagtgacaa ctctcgatat gttgatcatt tatttggact tgaacgtcgt atattcgatg 1020 caattgatag tggatacttt ttgaaaccaa acggtgaaaa aatgatgctt gacaaaccag 1080 aaagtataga gtatttagga aatcttattc aaaataataa ggatactatg gggaacacat 1140 acttttacgg attgcttgaa acttttggtc gtaagctgct tagtggttca ttcgaaagca 1200 tagaatcttt taccagaatt cctggtgtaa tggagctatt cgaaacagca atgcgtgacc 1260 caatgtttta cgtcttctat aagcgtatta cattcttcta tgaagcattt atcaataaaa 1320 tgccacgata caagaaatct gatttagtgt ttgacggagt caaattcgaa agcgtcgaaa 1380 tggataagtt gttaacgtat tttgatatgt ttactgccga tattacaaat gctgttgatg 1440 ttgctttgga tacgcaagac aatattaaat ctaaagttga atttaaagct caggttccac 1500 gattgaatca cgtgccattt aatgttcaca tgaaacttac ctcaaataaa gtacaaaaag 1560 cagtaataac gatgtttgtt ggaccaaaat acgatagctt tggaaagtat atagatttta 1620 cgcaaaatcg aaataattat tgggaaatcg ataggtggat tgttgactta aaaaaaggtg 1680 caaatgtaat tgacagaaaa tcaacagaat tttcttggtt tgttaaagat agaacaactt 1740 attatgaact ttacaaagga cttatgacag caataaatgg aggcgacaaa ttcccattgg 1800 atatgtccga agcacactgc ggatttccat ctagattaat gttaccaaga ggacgcgttg 1860 gtggtcttcc tgttcaattc ttctttatgg tttatccata tcatgcacca tctgtagaac 1920 gctttactgg attcgacccg tcagtctcgt gtggcgttgg cagtggtgct cgctatttag 1980 attcaatgcc attcggatat ccatttaatc gacagttcga atatcatttt aaactgacag 2040 aaaatatgat tttgcatgag accttcattc atcataaggc aaatgatgaa attatggtgc 2100 catactaagt tttcaaaaca tattcaaaaa tattacactt acatagagtt ttgatatttt 2160 tttcacgttc caaatttttc cgtaaagcaa aatacaataa aaataaaaat ttctgattta 2220 aaa 2223 <210> 17 <211> 538 <212> DNA <213> Chironomus riparius <400> 17 tcgagttttt tttttttttt ttttaggttt tccttaattg catggaattt tcacattttc 60 cgctttattt gaatatgaaa tgatgttaat ttcataattt cgcaatagta acagtagtaa 120 ggatacagaa tatttttttt gctaaagctt acttcagctt cttctttggg cgcaagttat 180 tcgtgtgtcc gcactttgtc ttacgacaat ttgttgcgcg aggatgtaaa cgagcatagc 240 acttacggca aatcatttta tcgcaattgt atttctgagc caagatacgt agagatggtt 300 caataatacc accacgaaga cgaagaacca aatgcaatgt tgattccttt tggatattgt 360 agtcactcaa tgtacgtcca tcttccaatt gtttaccagc gaagattaaa cgttgttgat 420 ctggtggaat tccttctttg tcttggatct ttgccttaac attttcgata gtgtcagaag 480 gctcaacttc caaagtgatg gttttaccag ttaatgtttt gacgaagatt tgcatctt 538 <210> 18 <211> 2276 <212> DNA <213> Chironomus riparius <400> 18 acgagtgcga tagtaaagtc gcgtcgtcca gctcagattg ttcggtatat ttaatttagg 60 tttaaaattc ttgtagataa cttgaagttt gtgtgaaaaa agtcagagta aaaaagcaaa 120 aaaatgtatc acttgaagaa tatcgcaaaa tcttctttgg tcgccaagga attgacaaga 180 gctttaccag tcgccttaag ttcgaaccaa caatgccgtg gatatacagg acatcaaatt 240 ccggaacgtt tacaacatat tcctactgcc gctaatccat tattcttcga tatggttgaa 300 tactttttcc atcgtgggtg tcaagttgtt gaggattcct taatcgaaaa tatgaaggaa 360 cgatcatcat tagaagagaa acgtaacaaa gttcgtggta tcttgagttt gatgcaagct 420 tgcgatcata ttattgaaat tactttccca ctccgacgtg ataatggtgt ttatgaaaac 480 atcacaggat atcgtgcaca acattcaagc catcgtacac caacaaaagg aggtattcgt 540 ttctctatgg acgtatgtcg tgatgaggtg aaagcattgt cagcattgat gacattcaag 600 tgcgcttgtg ttgatgttcc attcggtggt gctaaagctg gtattaaaat taatccaaag 660 gaatattcag aacatgaatt ggagaagatt acacgtcgtt ttgcccttga attggcaaag 720 aaaggatttt tggcaccagg cattgatgtc ccagctccag atatgggaac tggcgaacgt 780 gaaatgagtt ggatcgctga tacatatgct aagactgttg gatatcaaga tatcaatgca 840 cacgcctgcg ttactggcaa accaatcaat caaggtggta ttcatggacg tgtttcagct 900 actggacgtg gtgttttcca cggaattgaa aacttcatca atgaagccgc ttacatgagc 960 caaattggaa caactccagg ttggggcgga aagacattca ttgtccaagg tttcggtaat 1020 gtcggtcttc acacatgccg ttatttgaca agagctggtg ctgcttgtat tggtattatt 1080 gaatatgatg gctcaatcta taatccagaa ggtattgacc caaaggcatt agaagattac 1140 cgtaatgaac atggaacaat tgttggtttc ccaggagcta agccatacga aggtgaaagc 1200 ttaatgttcg aagaatgcga catcttcgtc ccagctgctg ttgaaaaagt tattacaagt 1260 gaaaatgctc ataagattaa ggcaaagatt attgctgaag cagccaatgg accaacaact 1320 ccagccgctg aaaagatcct tattgaacgt aacgttcttg tcattcccga tttatatatc 1380 aatgctggtg gtgttacagt ttccttcttt gaatggctca aaaatcttaa tcatgtctca 1440 tatggtcgtc ttacatttaa atatgaacgt gaatcaaatt atcacctctt agaaagtgtc 1500 caagaatcat tagaaagacg ttttggacgt gtcggtggaa gaattccagt cacaccatca 1560 gaagctttcc aaaagagaat ttctggtgca tcagagaaag acattgtaca ttctggactc 1620 gactacacta tggaacgttc agcccgcaac atcatgagaa ccgctgcacg cttcaatctt 1680 ggtcttgact tgagaacagc cgcttacatt aattcaattg agaaaatctt cacaacatac 1740 cgtgatgctg gattagcatt ctaaattcga tgaatatcta tctgaactgc tggagaatga 1800 agcatacaca cacattcaaa ttatatttca acaagacatt taagccgtat ttcttttttt 1860 ttcgttctga tttgttaact ttattttaat ttttccaaat tttttgacat ttctcctgat 1920 ttttgtgaac cttgccatgt gtacttttta atcaattaaa cgtgaaaaaa atattgagtc 1980 tttttcttga tagattgagt atgaattgta gcttaaaagt gtccacatga tatgtgtgga 2040 ttagattaac attgagtaat taggtttgtt gctatgttgt ctatgagaga tgagagttgc 2100 ttcctgtttt acgcatggaa gttaattaat ttttccctct atgaaattta tgcaagttaa 2160 agcaattttc atcctatctg ccacttttta aatgcaaatg aagcctaaat cttttacatt 2220 atgtactcag aaatatttgg agaataaaag aatgatttgt tgttttaatt atagac 2276 <210> 19 <211> 1833 <212> DNA <213> Chironomus riparius <400> 19 acgagtgcgt cgaacttgtc ggacttgaaa attgaaaaaa tattaattta ttaaacttta 60 gaggaattag tcataaatct ttccaaaatg gcgtcgagtc ttttaattat ttgtgcaaca 120 ctagttttgc tgtttatatt tacatatttt aagaaaaagt atgaatattt taaagatcgc 180 ggcattccaa atccaacacc atattggcca cttggaaatt tttggaaagt tggacttaca 240 gtgcacttca ttcatcgtgt aaatgagatt tataatcgat ttaagaaaag tggaaaagtt 300 tgtggatttt atatctttac tactcctgtt tatgtcgtca cagacattga tctgattaag 360 aatattctcg taaaggactt tgaatcattc catgatcgag gtctttacta taacgaaaac 420 agcgatcctt tatcagccca catattttcg gttgcaggtg aacattggaa aactctcaga 480 caaaaaatga caacaacatt ttcgagtggt aaaatcaagg gaatgatgcc gacattaacg 540 aaaatttcac atgagttaat tgaagttgtt ggtgaggctg ctgataataa aacacatgtt 600 aattaccgtg atttggcatc aagattcatg tgtgaaatta ttggagaaat tgcttttgga 660 ctgaaatgca atgcccttcg tgatccaaat tgtgaaatga tgttactgag cgacttcctc 720 agttttaagg acatgacaca acgtctttca tacttttttg ccaattcttg tcaagacttg 780 tgtcgcacat tgaatattca aatcacaccg aaaagaattc aagacttttt ccttcgtgtt 840 gtcaacgaag ctgttgagta tcgtgaaaag aacaagattc ggcgaaatga ctatttcgat 900 atgctgcttc aattgaagaa tgtcggtaaa cttgaaggcg atgtaacaaa tcatggaaaa 960 atttcctaca cagatctaac agctgcgtgc tttgttttct ttttggctgg atttgagacg 1020 tcatcgacag ctttgtcgta tgccctttat gagttggcca ttaatcaaga actacaggat 1080 cgaacacgcg atgaaattga aaaagtcatt caagaatatg atggtgaaat aagttatgaa 1140 gctattatga atatggattt tgctgaccat gtaattaatg agtctttgag gaagtacaca 1200 cccggtaaca ttcttatgcg tcgttgtaca aaagattaca aagttcccgg tactgatttg 1260 actattgaaa aaggaaaata tgtcttctta cccatgcatg cgattcataa tgatccacaa 1320 tacttcccag aaccagagaa attcaatcct gatagatttg caccagaagc tgttaaggaa 1380 cgaaatccat ttacattcct tccattcgga gaaggtccaa gaatttgcat ggggcttcga 1440 tttggtcaaa tccaaacaag acttggatta gcttcaatcc ttagaaatca tcgcgtacat 1500 ttgagcgaaa agacaatcca tccaattgga cttgatccta aagatgttac aatgatgcca 1560 aggggtggaa tttggttgaa gacagagaga ctttaaattg attgacattt attaggaata 1620 atgattattt agggtttatg acattataga agtggaaatg aatgaagttt caagacaaca 1680 cagcgcataa atttttctac ttatcatgta aattatattc ttaaattttg tggattctaa 1740 acttatgaac attacgatta caacttctgt attattggaa gtgatcgaac aacttttgga 1800 attgaaataa aaatatttaa ctacataaaa aaa 1833 <210> 20 <211> 1402 <212> DNA <213> Chironomus riparius <400> 20 taattcagaa tttaacattt ttattaaact tccgaacagg aaataaatcg tgtgtcgtta 60 catcaatagt atcccaattc gaataaattg catgtttttt aagtaaattt ccctagtaac 120 cttatctaag aacagataat taatttcaaa taaattcaac aaattaaagc aaatttttgt 180 ttaaggaatc gaagaacaga agggagtctt ttgtggacat aaataatatt atttataatt 240 atcagcagtt caattgtttg tgttacttgc tattcagtaa gaaatttact tcttgttgat 300 gaattcttca ccaacttgga tgttcttctt caattcagga atagcctttg acaaaagttc 360 ctgttcgaat gcattcaatg atcccatacc caaattcttt ttaattccat tctttccgag 420 taaaagtggt gttgagaaat attttgcttc ggtgatgtct gaacgaacat aagcacattc 480 aatgacattt tgttcgcctt gtaatgcacg aataagagaa attgcgaaac gagctccagc 540 ataagccatt gagagtgttg ctgatccagc accagccttt gcctttacaa cctcagttcc 600 agcttcttgg atacgttctg tgagagcctt aattttgtct gctgaaaagc ttactgatgg 660 tgtgcattgt gagaacaatg gcatgattgt aacacctgaa tgtccaccaa taactggaat 720 attgacttta gatggttcaa caccagcggc ttcaccaata aaagcattag cacgaacaac 780 atcgagtgtt gagacaccga agattctctt tggatctaaa actccaagtt tagcaagagc 840 ttcgcttgca attggaactg tcgagttgac tggatttgag atgataccaa ttaaggcttt 900 tgggcaggct tttccacatg cattagctaa atcgcgaacg attgatgcat ttgtgttgaa 960 taagtcatca cgagtcatgc ctggcttacg tggaactcca gcaggaataa taacaacatc 1020 agcaccttgt aaagcctttt ccaaattttc aggtccatta aatccaacaa ctttgcttct 1080 agtgtcaatg tgtgacaaat cagcggcaac tccgggtgtg tgtacaatat catacaaaga 1140 caattcagtg atcaaaggac tttgcttcaa taacaatgat aagggctgtc caatgccacc 1200 tgatgcaccg caaacagcta ctttgacatt gttttgatgt gatgttgaga agttcttggc 1260 tccttgttgg gcagcgattc tgaagactct taagaacatg ttgatgctga attaatttga 1320 tgtatggatg tgaaagagaa aattcgaaaa caatcggtag caaatagcaa aactcgacta 1380 aaaggtccaa taagaatgtt tg 1402 <210> 21 <211> 1793 <212> DNA <213> Chironomus riparius <400> 21 tttttttttt ttttaagatt tttcaaatct aatctatttc taaaacttta ttacacattt 60 tgtgcttctt cttcttgcac tcatttttgc ttaacagatt ctgataaaca ttaactgcca 120 atccaaaaat tattattgcg aaaacataaa aattctagtt aaaaattaaa acaatatttt 180 taatgaataa aaaatcaaca gtcaaattgg cgaactagcg actttgtaaa tatgatgaca 240 ctaccacgat aatagcaaga ggtctgttca gtagaagaac catttaatgg tccaaaattc 300 tcaagtttcc agagacaatc ttgttttcaa gaatagcacc tggtggaata tcaatacgat 360 ctccatgatt tgcaataata atgacagttc cacgaagtga cacgccacga ccaaaagtca 420 catctcccga tacagtcaag tgatccaatt caataatgtc aggaatattt gcaaaacgtc 480 ccaaaaattc tttgactttg ctgaaatgat tgtcacccag tttaaccaat ggcgtagttg 540 gaaacattct ttgtggagac ataacaagag agccagattt taaatgatac aaatttgaca 600 ttacaagcaa cagatcagaa gttttcttta caggcaagaa acgagaacga ggaacattaa 660 ttccaatggc attttcaaaa catttcattg cagcaccaac agccgtctcc aattgaatga 720 ctctcaagcc attatctaat gtcttattgt tcacaataat ttccgtattc attgatttct 780 cgtttaatac tctttcaatt gcatccaatt tcgcccaaat attattagtg ttgaagaatt 840 tgaaggtctt gactgatttg aactcatcaa catgctcgtt tggaacttga gcaatttcca 900 aaagtctcaa tttgttttca tactgaataa gagtaccacc cttaacgtca gcacgagtct 960 tatcagtgac ttccataaca aattcatgac aaactccatc tgtttcgccg actaagcgat 1020 taaggatgcc aagatcaatt gttgcgccca agttatcaat atttgacagg aacaaatatt 1080 cacgtccttc attgatgaat tgcttcaata agccagactt ttggaacgat tcgtagaagt 1140 ctccgtgacc tggcggatac catgcttcca tattatcttc aatctcatat tttcgtgcaa 1200 caggtagaag agaatcgcgt gaaatacgtg gaaatgcgct ttgattgaaa ctataaatgt 1260 tgacttgaaa gcctttatat tttcgtacaa ttttttccgt atcctcatct gtattaaacg 1320 agttcattaa taccaaagga acatttgcat tgtacgtctt attgagactt tcaatctgct 1380 gtacagtcaa gtccaaaaat gtcaagtcat tccttactgg aatcacactt tttggtccgt 1440 gacatcccat ggatgttccc aaaccaccgt tgagttttac aacaacaagt ttgttgagca 1500 ttaaacggat ttggtcattg tttgttggcg tttggagatg actataatcc tttacggctc 1560 cttcaggaag tttttcaatt ctattccatg caactgatgg tccatcttct tgcaaaaacc 1620 ttccgaaaag cccagcaaaa cttgtcattt cctcacgaaa tgcatctttc ttatctgcag 1680 gagatgtttc aatcagctta tcaagttcac gttccaattg cttcaaagca tcccgcttag 1740 tgacttcgtg aaattctttt gcatcacttg gaacacgaga gtgtgtacga ctc 1793 <210> 22 <211> 1662 <212> DNA <213> Chironomus riparius <400> 22 aaggcctgca cactgaaaaa ttttaaagag ccgcggtaat ttgaccgtgc aaaggtagca 60 taatcattag ttttttaatt gaagactgga atgaaaggtt taataagaaa taagctttat 120 tattttaaaa ataaatgaat ttattatttt agtaaaaaaa ctaaaataca gataaaagac 180 gataagaccc tataaagctt tataataata acttatataa tttttttaga ataatataaa 240 ttttatttgt taattattta attggggtga tttaaagatt tatttaactc tttataattt 300 tttttaataa tagttaataa aaagatcctg aaattttgga tttaaaaatt aagttacttt 360 agggataaca gcgtaatttt tttggagagt tcaaatcgat aaaagagttt gcgacctcga 420 tgttggatta agatataatt ttaggtgtag aagtttaaat ttttagtctg ttcgactatt 480 tattcttaca tgatctgagt tcaaaccggt ttaagccagg ttggtttcta tctttattta 540 aatattatat tttagtacga aaggaccaaa tataaaaaat attatttttg tcgaatgaat 600 aaaattaatt taaaaaataa attatttata aaatttatta aaaactattt tggtcagata 660 agtgcaataa atttagaatt tatgtatata ggttaaaatc tataaatagt attgattttt 720 aatttagatt tatttatacc atttttaaga agtttaatat taattatttt tgtaatagta 780 agtgttgctt ttttgacttt attagaacgt aaagttttag gctatattca aattcgaaaa 840 gggcctaata aaattggata ttttggtatt ttacaaccat tttgtgatgc aattaagtta 900 tttacaaaag aacaaatcct acctttttta tctaatagaa ttatttatta ttttgcccct 960 attttttctt tatttttatc cttatttatt tggttatgta tacctttttt agtaaaactt 1020 tattctttta atttaggtat attatttttt tttgttgtat aagatttggt gtatatatag 1080 ttatattagc gggatgatct tcaaattctg catatgcatt attagggtca ttacgttcta 1140 ttgcccaaac aatttcttat gaagttgctt tggttttgat attattatct gttttatttt 1200 taattggaag ttttaattta gtttattttg aattatttca aaaaaacata tgatttttaa 1260 ttttattttt tcctattggt ataatatgga taatttcaag tttggcagaa acaaatcgga 1320 ctcctttcga ttttgcggaa ggagaatcag aattagtttc tgggtttaat gtggagtacg 1380 gggctggtgg atttgcttta atttttttag ctgagtatgc cagtatttta ttcataagta 1440 tattattttc tatattattt ttagggtctc aggtatactc ttttttcttt tttataaagt 1500 taaatagaat tgcttttttg tttatttgag tgcggggggc tcttccacgt tatcgttatg 1560 ataaattaat aaatatagct tgaaagagat atttgccaag tgtactttat tttttatttt 1620 tattcgttgg gttatttatt tttatttttt aaaaaaaaaa aa 1662 <210> 23 <211> 901 <212> DNA <213> Chironomus riparius <400> 23 tttagttttt ttttttttgt agtttcttac tatttttttt tattcattat ttaatgctta 60 taatttttat ctcaaagata ttacatgaca agatagatag gggatctttt agaacaaaag 120 gcaaatcaac tccaaataaa ctttattttt aataatagta cgaagaataa aaagattaga 180 aacattacgc aattttaata gcttgcgtgc tataaactgg gatgaaagaa tttacaatta 240 attcattaaa tgactgatga acacatcttt tgcatcaagt cttgtgttat cgtaaaataa 300 atccagtttt gcaaaagaaa aacgtttaaa ggttgcttct taacgaaaac aacgcattaa 360 taatagtagt tatcaataac atacttttca cgactttttt gataataatt tttaagctgt 420 tgatgacgaa gaaagaaata ttaagtagtg ttgattttcg tagtagttta atgattggca 480 gagatttttt atgaatgctt ggaaatactt aattctttgc ttccttctta tttttctcag 540 agttttgaag ttttgttaca atcttacgag tatgtccact tggatcattg tgatttgtat 600 gagctgctac ctcagccttt gttctgcctt tacctgaatg tccactatac atgtgtttat 660 cattctcgaa ctcatattgt tcatatttct catagtctgg attgctgttt ttggggctac 720 tcatgttgat aaagttattt gtttcaagaa taagaaaaat ctaccagtta aatcactaaa 780 atatataatt taatatcact aaattggctt tacactcgaa gttttgtact agtaatcagc 840 tttttaagtc ttttaatctt attgattaat taatatttgc tcaagaaacg atgttaatcg 900 a 901 <210> 24 <211> 1302 <212> DNA <213> Chironomus riparius <400> 24 acgctcgaca atcattttca attgtagatt gcgaatattt gttgactttg tcatgcgttg 60 ccttttcaaa attccatcac tttttgtttt cattttgata acaaattttg taagttcaag 120 tgtaaatcat gaacgtgatc ttggggattt ttgtgtgctg aaaaacagtg taagaggtgt 180 ctgtaaggaa atacaggatt gtgattatgc aaaagctttg tttaagctga gaaaaaatgg 240 agaaattcta caggatcgat gtaaatttca ttcaaacacg ccttacgtgt gctgtccagc 300 atcatcagaa gtaagaattg atgaagagca tgcaacggaa aaagtcataa catcttcatt 360 tagtgaatcg aaatataaga aggctttatg tgaaaatgaa actccagaaa ttgtattaga 420 tctgcatatt gttggtggtg aagaagcaga agtcggagaa tttccataca tagcagcaat 480 tggatataca aaagcttctg aaccagattc aacagatatc gagtatcttt gtggtgggtc 540 actaattgct gatgatattg taattactgc tgcacattgt gtaaatagga ttgaaatcat 600 acctaaagtt gttaaattag gaagaacatc gctagtgcct agtcaacatg acttttctga 660 tggtgatgat gtggaaatag agtacttcaa aatacatcca aacttttcgc aaggatcaaa 720 acacaatgat attgctttaa tgaagcttaa gcgtccattt aacagttcta ttgcattgat 780 caagcctatt tgtctttcta ccaaagacga aaatttgcca tttaatttca caatcgctgg 840 ttttggcaga aatgatatca atactcaaaa aacatctaat tggttattga aagctacagt 900 taacgaatat ccattggaaa aatgtcaaga aatatttttg aatgcatcga tgaataccaa 960 gaaaattata gactcacatt tttgtgcgtt tggacataaa ggagctgaca gttgtcaagg 1020 agattcagga ggaccattaa gctacgaaat accatttgga gggggtaaat tgacgtactt 1080 atacggcgtc gtttcaactg gaattggatg tggatcaact attcctggca tttacacaaa 1140 ggttaataaa tttttggatt ggattgaaac tgaaatgaat ttgatggaat aaaaaaaata 1200 aaattgattt gcgtgaaatt tcataaagta gattttgcaa gaatgtgaaa aaaacttttt 1260 tgactcaaat gtaatcgaca tttaaaataa tacaaaataa tt 1302 <210> 25 <211> 1584 <212> DNA <213> Chironomus riparius <400> 25 acgagtgcgc aaagatggag gttctgtcga caattggttt attaattgta cttgctgtga 60 ctatttatgg atttctgctg catcaatatt catactggaa gcgacgaaat gttccgtaca 120 tagaaccaca tttgatttat ggaaatgcca aaggagttga gtctaaattt catcatacaa 180 agttttggat tgatttgtac caaaagttaa agtctaaagg acctgttgct gggatttatg 240 tctttttcta tccaattgcc atcgtcacag acttagatct agtcaagcag atacttgtta 300 aagactttaa tgtgtttgtg aatcgaggac attacctgaa cctcaaagat gatccaatta 360 gtgctcattt gttcaatatt gaagatgaat catggaaatt tatgaggaac aagttatcgc 420 cggcattcag cagtgggaaa cttaaagcaa tgttcccaac catcattaaa cttgctgaca 480 aattcacaga aattgctgaa accaacactt cattggatgt ccgagactta ggtgctagat 540 tcgctagtga tgtcctcgga tcggttggct ttggaatcga gtgcaatgca atgtccacaa 600 atgactcaga ataccacaaa atagcaacaa acttcatgaa taatttgaga tttatgagca 660 aaactttgtt ggaaagcaat cgaaagttct tcaattttat aagattcaca aacattgatg 720 actttaccag gacattctac acaacaattg tcgacaaaat gctgcaatat cgcgaggaaa 780 attctgtaaa ccgaacagac tttttaaatt ttatgattca cgagaaagac tcatcaagac 840 aactgactag aattgaaatg gcatctcaaa ctggagtgtt tcttgttgct ggccatgaag 900 ttgttgtatc gctgctttca cattgtcttt atgaactggc attaagaaaa gatttacagg 960 agaatgctag aaattcaata aagcaggtgc ttgatgagtt taatggagag ttcagctatg 1020 agtcggttta taagatggat tatattgaac agtgcattta tgaaactgcc agaaaatatt 1080 tcatcgctgt tagcgttcgt cgtgttgtca caagagacta taaagtgcca aatactgaca 1140 ttgttctaga gaaaggcaca gtcgttcaaa ttccagtcta cgcaatccat caagatccac 1200 aaatctaccc agaacctgac aaatgggatc ctgagagatt ctcaccagaa aatgtagaaa 1260 aacgacataa atttgcattc ttagcattcg gagaaggtcc acggatgtgc attggaaaaa 1320 ggcttggagt tttcatccta aagacagttt tggcaaaatt cttaatgagc tatgaattca 1380 accttgacac aaaaaagacg tcagttccaa tcaagcattg cataaagaag cttttcctta 1440 caccttcaga cggcattcat atcaatgtca ctaagtcaaa atttaattaa taaagaaaag 1500 ctgtaagata tgtaattttt caaaattgtg ataaaattta aaaaataaaa attgagataa 1560 aaaagttata ataaattcaa aaaa 1584 <210> 26 <211> 2006 <212> DNA <213> Chironomus riparius <400> 26 acttttttaa atcttgctaa ttcataaaca agactcttcg taagcagccc aaaactactt 60 caacttttat tatttttatt gttttgaatt tctgcatcca tttaatggaa tctgaaacac 120 aatattattt ttataatttt aacatattat gttgctcatt taagtattca atagcataaa 180 attattaatt tttaaggaat ctaaatgtac gtgcgcgtaa tataattata aattgatgag 240 atttcctcta atgtctttac caatttgact taatgaagtt aatcaagcca tttgttgatg 300 catcatgact tgagacttta tcattgttgg ccaagtcacc ttcaattgcc tttgccaatg 360 cctttccaag ttcaacaccc cattgatcaa atgaattgac atcccagatg ataccttgtg 420 tgaaaatctt ctgctcataa atagcaatga gggcaccgag agtgaatggt gttactttct 480 gaacaacaat tgagtttgtt ggacgatttc cagtaaaaac tttatgtgga acgagatttt 540 ccaatttttc tcccttcata ccggcttttt caagttcacc tcttacttgt tgttctgtct 600 tgccgcacat gagtgcctca gtttgtgcca aaaagttggc aagaatcaat ttatgatgta 660 caccgccagc aattggattg tgagtttgag ctggggcaat gaaatcacat ggaatcaatc 720 gtgtgccctg atgaatgagc tgataaaagg catgctgacc gttagttcca ggctcacccc 780 atacgatcgg tccagtgctg taatcaacgc gtttgccgct ctttgcaact cctttaccat 840 tactttccat gtcgccttgt tggaagtatg cagcgaatct atgcaagtat tggtcatatg 900 gcaaaagtgc tgttgtttca gcgccataga aatttgaata ccaaacaccc aaaagtgcga 960 gaattactgg agcattttga cataaaggtg ctgtttggaa atgctgatca atgaagtaag 1020 caccttcttg caatttaaca aagttgtcga atccaataga gagagaaatt gacaaaccaa 1080 tagcggacca gagtgaataa cgtccaccaa caaagtccca gaatgcaaac atgtttttgg 1140 catcaatgcc aaacgctgtt gcaagtttct cgtttgttga caatgcaaca aagtgtttag 1200 caacgtgact tggatccttt gcaacttcca agaaccagtt cttagcagat gtggcattag 1260 taattgtttc ttgtgtcgta aatgtctttg atgctataat gaataaagtt gtttcagcat 1320 tgattttctt taaggtttca gctaaatgag ttccatcaat gtttgaaacg aaatgcatat 1380 ttaatccagt tgaatatggt tttaaagctt ctgtgaccat tagaggtccg agatcagatc 1440 caccgatacc aatattaacg atatcagtca ttttcttgcc tgtgtatccc ttccattcac 1500 cactgacgac ttgattagtg aattccttca tatgatccaa aacagcaaga acttcaggca 1560 taacatcttt tccatcaact aatacaggct ttcctgactt attacgcaat gctgtatgca 1620 atactgcacg atcttcggta atattgatat gttcaccacc aaacatttgc tcacgagctt 1680 ctttaagtcc acgagagttg gcaagttcta ataacaattt ccaagcatcc tctgtgatgc 1740 gattctttga gtaatccaat aaaatgtcgc cgtcatttgg cgttttcaac actaaactaa 1800 atttatcaaa gcgttttgga tcgttgtcaa acaattcttt aatgttgatc tttgatccat 1860 ttgcattgta atattcttgg attttctgat aggtagcatc agcagtaagt aatggtttcg 1920 acatgattaa attgacaata aacttttaaa acgacagcaa agaagtaaat ttcaccaact 1980 gtcttactcg gcaaacacgc actcgt 2006 <210> 27 <211> 1769 <212> DNA <213> Chironomus riparius <400> 27 acgagtgcgt ttctcgtttt agctgctgtg cttgcaattg cctacgggca ggatcgtcca 60 attgtgacaa ttccccaagg tcaacttcag ggaataagaa catcgtcggg tattactcaa 120 gctacatacc ttgcattcaa aggaattcct tatgcagaac caccagttgg tgccttgaga 180 tttagaaatc ccgttgctca tcgtggatgg agtggagtac gtgatgcttc ggcacatgga 240 aattcatgtc caagttctgg atggtttgga ttggaggcag gcggtggaga agattgtttg 300 ttccttaatg tttatacaca atcattgact gccaatagac ccgttatggt ttggatacac 360 ggtggttcct ttactggcgg tagtggtgat agcttcattt atggaccaga ttttttcatt 420 aacgacggaa acgttgttgt tgttaccata aattatcgtc ttggagtctt gggcttcctt 480 tcaactggag atgctgctgc tcaaggaaat tggggcatga aagacatgat tacagcttta 540 caatgggtca gaaataatat cgcaagcttc ggtggaaatg tcaatcaaat tacagttttt 600 ggtgaaagtg ctggctcagt cggtgttcat tatcttctct tgacaagact tggcactgga 660 ctcttcaatc gcgcgatcat gcagtcaggt gttacagtgg caccatgggg cttccagcca 720 aatccattga gtgaagcaac aaatcttgga cgtagattgg gacttacttg gtcatcaaca 780 actgatcttg tcaatcaatt gagaaatatt ccaccccaaa gacttgttga taatcaaggt 840 ggatggcttg acttgacaat tccacgtgga ttctcaccat ttgaattcgt accaaatgta 900 gaaccagcaa attcacctga aagtcgattc cttactgata caccagtcaa cattatgaat 960 cgtggtgata tccttacaat gccaatcatc attggattta tgtctgtcga aagtttgttc 1020 atggttcgtg aacatttgtt tgatgacaca actttagctc aatttgcaac aaatccacac 1080 ttctacgtac atccatcatt caaccttgct ccaactcaaa caacacaggt taatgaagtc 1140 gcaacgacaa tgagaaatat gtactttaat ggcggtcatc caactatgga aactcgcttc 1200 aattattcgc aatttatgag cgatcaccat ttcgcatact caatcgatcg atgtgttcgt 1260 tatcatgcac gacgtcagac tcaaccaatc tactattata aattcgactt tgatggttca 1320 ttaaatatga ttaagagatt attgcttttg acggactggc ctggtgcagt tcatgctgat 1380 gacattttct acatgtttga cgttacacgc ttcccacttc caatgctccc aacaaatgct 1440 gctgccttga caagacgcag aatggtcaga atgtggacaa actttgcaaa ctttggagat 1500 ccaacaccag tcactgactc attgattcca accagatgga gccgttatac attggctaat 1560 gaagaatatt tggacattaa tgaaggcttg tctgttagaa gtgcacctta tgatggtaga 1620 cttcatccat ggcattcttt ccaagataga tttaatccat cttatcttag ttaagttttt 1680 gagagttcaa aggaaatgac ttatgagata tttgatattg ttccttaatt tacaataaac 1740 agctgcaaat atttaaaaaa aaaaaaaaa 1769 <210> 28 <211> 1268 <212> DNA <213> Chironomus riparius <400> 28 agcaaaaaat cattcttgaa ctttgtgatt aaatcaacaa gtaatattaa ctagatatga 60 ctgaaggatc tataataact tgtcttgctg ctgttgcttg ggcaccaaag gaaccgctaa 120 aaattgagaa aattcaagtt gagcccccaa gagcaggaga agttagagtt aaaatttatg 180 ctaccggagt ttgtcatact gatgcatata cactaagtgg tattgatcca gaaggtctat 240 ttccatgcat tttgggtcat gaaggtgctg gaattgtgga atcagttggc gaaggagtca 300 cacagtttaa ggctggagat catgtcattc cactgtatac acctcaatgt aaggaatgta 360 attattgctt gaacaagaaa acaaatctgt gccagaaaat tcgtttgact caaggacgtg 420 gtctgatgcc tgataataca agcagattta gctgcaatgg aaagacactg tatcatttta 480 tgggatgctc aacattcagt gaatatacag ttgttgctga aatttcaatt tgtaaaattg 540 ctgatgcagc accactcgat aaagtctgtt tattaggatg tggaattcca actggatatg 600 gagcagctat taatacagcc ggcgttgaaa aagattctgt ttgcgctata tggggacttg 660 gtgctgttgg attggctgtt attatgggct gtaaagatgc cggtgcgaag aaaatcattg 720 gaattgacat taatccagat aaatttaatg tagcaaagag ttttggtgct acagattttg 780 tcaatccaaa ggacttaggt gacaagacaa tccaagaata tctatttgat aattttgatc 840 aaggacctga ttatacattt gaatgcattg gaaatgtcca aactatgcgt caagctttag 900 aaagtgctca taaaggatgg ggagtaagtg ttattatcgg agtagctgga gctggccaag 960 aaatttcaac tcgtccattc caacttgtta ctggtcgtgt ctggaaagga acagcattcg 1020 gtggatacaa gagtgttgaa agtgtcccaa aattagtcga tcgctatctt gctaaggact 1080 ttaaaattga tgaatttatt acacatgatt taccattgga taagattaat gaggcatttg 1140 atttgatgca tgacggaaag agcattcgag ctataattca cttaaattaa atttatttgt 1200 gctaattttt tatgaatttt tatgggttct aaatactgtc gatatactca cgacttttga 1260 atgataat 1268 <210> 29 <211> 885 <212> DNA <213> Chironomus riparius <400> 29 gttgagtata gaaaatatgt ttctcagttg ctcgtatata ttcacatagt ttagtcatga 60 gtcgtaaatt gccgatgact ttatatcaca attgcttcag cccaccgtca cgcatggctg 120 tgctggctgt aagaaatttg gcactcgaaa ttgacgttag aaatattgac atctacaaag 180 gtgaacaaaa tactccagaa tatctcagag ttaatccttt gcatcaagtt ccatgcttgg 240 ttcatgaaaa ttttgtgctg acggaatcaa gagccatcat tatgtacctt gcaagtctca 300 ctgactcacc gctttatcca acaaacgatt taaagaaacg tgcccttgtt gactcacgat 360 tattttttga tgcgacgaac tcatttgtgg ctgtcaaaaa ttttgcgcga ccagttcttc 420 gatcaggcgt caaaaagatt ccatcagtag ctcgagaaga catcaaaatt ttactcaaca 480 cactcgactc gttccttgat aaatctgagt ggtttgcagg cgatgagatg acaatagctg 540 atttagcgat actcgcgagc gttggaacta taaaatgctg gggtgtgaac tttaaagaat 600 tcccgaaact aaatggatgg tttgagaaat gtaagaactt gcctggattc tctgaaaatc 660 atgaaggaag tcaagcattg gctgagaagt tatcgaaatt gctggaagag cctttatgga 720 agtgatgatt aaacatcatt gcaattgaaa tttttttacg aacaagcgaa gttcttcaat 780 tttaataaaa aatttgttat cgagaccttt ttgttatctc ttacctcaca taatttttga 840 gtagattaaa cgtatataaa caaaaaacat atattagtaa aaaaa 885 <210> 30 <211> 1755 <212> DNA <213> Chironomus riparius <400> 30 cgcttaaatt ttcgaaaata atattttaaa aatacataat atactgcata cgattagaca 60 aagtgagcat acttgagcat ttatcattta gtttatgttt tgaagaaaaa gagattcgac 120 aaattagaca gaagaagaag aagaaatttt ttatagaaat ggcactagca ataaaacggc 180 tttcttctat tccaaaacaa cagcttgcaa ttcttgcagt gccagtgtca caaaaatacc 240 aaaatattca ggtacgacat gcacaagttt cagcaccacc tcctcaaaca aaaacaaagt 300 ttggtggatt gtcagatcag gacaggatct ttacaaactt gtatgggcgt catgattgga 360 gattaaaggg tgcattaaag cgtggggatt ggtacaaaac aaaagaaatc ctcgataaag 420 gtgttgattg gattttgaat gaaatgaagg cttctggatt gcgtggacgt ggtggtgctg 480 gatttccaac aggaatgaaa tggtccttca tgaataaacc atcagacggt agaccaaaat 540 atcttgtcgt taatgctgat gaaggagaac caggaacttg caaggacaga gaaattatga 600 gacacgatcc tcacaaactg atcgaaggat gcttaattgc tggaaaggct atgggagcaa 660 gagctgcata tatttatatt cgtggtgaat tctacaatga agcatcaaac atgcagattg 720 caattgctga agcttatcaa gctggtctta ttggaaagaa tgcatgtaat tcaggctatg 780 attttgatgt ctttatgcat cgtggagctg gagcttatat ttgtggagaa gaaacagcat 840 tgattgagtc attggaaggt aaacaaggaa agccacgttt gaagcctcca ttcccagctg 900 atgtaggtgt ttttggatgt ccaacgactg tatcaaatgt tgaaactgtt gctgtagctc 960 caacaatttg tcgtcgtggt ggaatttggt ttgctggttt cggaaggact cgtaattcag 1020 gaacaaaatt attcaacatc tccggacatg tcaataatcc atgtacagta gaagaagaaa 1080 tgtcaattcc attaaaagaa ttgattgaac gtcattgcgg tggtgttcgt ggtggatggg 1140 ataatttgct tggaatcatt cctggtggaa gttctacccc aattattcca aaagatgttt 1200 gtgctgaagc attaatggat tttgatggac ttgttgcttg tcaaacatca cttggaactg 1260 ctgcaattat cgttatggac aaatcaactg acgtaattaa agctattgcc agattaattt 1320 ccttctataa acacgaaagt tgtggacaat gtacaccttg ccgtgaaggt gtttcgtgga 1380 tgaataaaat catgactcgt tttgtgagtg gaaatgcacg cccagaagaa attgatatgt 1440 tatgggaggt atctaaacaa attgaaggtc actcgatctg tgcattggga gatggtgcag 1500 catggcctgt tcaaggcttg attagacatt tcagacctgt tattgaaaag agaatgaagg 1560 atttcgaaca acgtgaagcc cttaagaatt aaatagattc aattatttga agtttcttaa 1620 ataacacaaa actatgtaaa ttatttgtct cagcgtgaaa atgtatctct ttttcacaat 1680 taaataaatt gatgattcat ggaatgttca agttgcttcc atgaattggg aaaaacgaaa 1740 aaaaaaaaaa aaaaa 1755 <210> 31 <211> 1784 <212> DNA <213> Chironomus riparius <400> 31 attcaaagta taatagctcc ctgcaaccag ggataaaggt agaatttata aatataatta 60 tttaaagtgt ctttatctga ttggtagaaa atttgtgcat tcaatcatta ttttttcctg 120 tatagtgctt ttgtttacaa tttgataatt tttatttttc atttatttta aatctttgtg 180 tgtgaattat ttttatttga cgtcgcaacg ataaaacatc atgaataaac tgccaatact 240 ctactcttac tggcgatcaa gttgcagttg gagagttcga atatgtctta atctcaaaga 300 agttccatat gacataaaac cgataagctt gatcaaatca ggcggtgagc agcattgcaa 360 tgaatacaga gaagtaaatc caatggaaca agttccagct ttacaaattg atggtcatac 420 actcgttgaa tcggtagcaa tcatgcaata tttagaagaa actcgtcccc aaagaccatt 480 attgccacaa gacttctata agcgagcaaa agtcagagag atatgtgaaa tcattgcatc 540 aggtatccag ccattgcaga atctgattgt tttaattcat gttggcgaag agaaaaagaa 600 ggaatgggct cagcactgga taacaagagg atttagagcc gttgaaaagc ttttatcaac 660 aagtgccggc aaatactgtg ttggtgatga aattactttg gcagattgtt gtttggttcc 720 tcaggttttt aacgcaagaa ggttccatgt cgatttacgg ccttatccaa taattctacg 780 aattgatcgt gaacttgaaa gtcatccggc atttagggca gctcatcctt cagcgcaacc 840 agattcacct ccagaaatta caaagtaaaa aatttcttca tttcaaatga gaacaaaaga 900 aaaaaatatt tttaaaaaac ttcaatcatt tttcttggac tttattaaac acttttcttt 960 cctttcaaaa attttaaaat aaaaagtcaa aaattataaa atttaacaca tgtgtgcttc 1020 atactcaaga cttgcagctt gtcgcatgta ttctaataca ggatttctta aacacatatc 1080 catttcttca acaagaaact ctccttcgac tgtaaatctt gtttgttccc attttaggag 1140 ttgtgttaca gctccaggcc attgagttgt ataaacttga tattgaccat tcaaatattc 1200 aataatctca tcctcatcat ccatcacatt tcctcttgat aaagcatcca ttacaatcaa 1260 attaataaaa agcacttctg attgaatatc agcgaacgtt ggataaaata catcacgcat 1320 ttgtgcttgc attgtattat ttgcacgaag tgaacagaca cgaacatcac gagtaatatc 1380 ctcagaaaga cgatctcttc tatttacgac accttgtaaa cattcagtta taccaccaag 1440 ttcattttct cttatttcaa gagcttcgtc aagttcaacg gatgctcttc taataaaatc 1500 taaatgttga ccgaaaagtt ccatcatctg aatgtaaatt gtttcaacca atgttacatg 1560 actaaggcga taccttctca tttgatagtt catttcagca tagaaaccgt taaaacgttg 1620 aaatttgtac attatctcgt cataaaaatc atcaacacga atatttccat cctctttgat 1680 ttcaggaact gatgacgcaa atgtctgtga tatgagagca agaacaacaa ttgcgataag 1740 acttttcatt ttcaaaactt aagcaataac ttcctgtcga gcgt 1784 <210> 32 <211> 1601 <212> DNA <213> Chironomus riparius <400> 32 cacttcaaag gaaaagaacg atgtcgtcac gttatctcat tgatgatcca aaattttcat 60 tccttaaaga tttgggaata gaaagaatca acaaaggagt ttataatgga gaatggtgtg 120 gaaatggaga agttacaaaa acatatgatc cagcaaccaa agaggttatc gctgaagttc 180 aaaatggaaa tgtcgaggac ctcaacaact gtataaaaat agcagatgat tcttataagc 240 attggaaaac tcttccagca ccattccgtg gagaaattgt aagacaaatt ggtgatgagc 300 ttagaaagta tcgcgagcca ttaggaaagt tggtctcact tgaaatggga aaaattcaag 360 ctgaaggaat tggtgaagtt caagaattta ttgacatttg tgactatgca actggactct 420 cacgcatgtt tgctggacaa gtcttacctt cagaaagaag tcagcatgtg atcattgaga 480 aatggaatcc acttggtgtt gttggtgtca tttcagcatt taatttccca aatgcagtgt 540 ttggatggaa tgcagctatt gcaatggttg ttggaaattc agtcttatgg aaaggagctc 600 catcgacacc acttatttct atagccacaa ctagaattgt aactgaagtc ttaaaacgca 660 ataatttacc accagttgtc acattatgtc aaggtggagt tgatgttggc aagaaaatgg 720 tagctgatga tcgtgttaag ctcctctcat tcactgggag tacaaatgtt ggccgtgaag 780 ttggtgtaga agtacaaaaa agattcggaa aagttctgct agaacttggt ggaaataacg 840 cattgattat caatgatgat gctgattttg atatggcact tgatgctgca ttcttcggat 900 gtattggaac agcaggacag cgatgcacca caacacgtcg tttaattatt catgagaaaa 960 tttatgatgc atttttagag aaattgattt taagatacaa gggactcatg aaacgagtcg 1020 gacatccact tgatgaagca acaattttgg gtcctcttca caatcaacaa gctgttgatg 1080 catacacaaa tacaatcaag gaagcaacta atcttggtgg taaaattgaa gtcggtggaa 1140 atatcatcga ccgtccagga tattttgtgg aaccaactat tgttactaat ctacctcacg 1200 atgcacctgt catcaagaga gaaacttttg ttccaattgt ttatgtttta aaagctaaga 1260 atttagatca agcaattgaa tggaataatg aggtagagca aggtctttcg tcagcattat 1320 ttactaaaaa tattggatct acattcaact ggatttctga aaatggttct gattgtggaa 1380 ttgtaaatat caacacatct ccatcaggcg cagagatcgg aggtgctttt ggtggtgaaa 1440 aggcaacagg aggtggtcgc gaaagtggat cagatgcatg gaaacaatat tgcagacgat 1500 caaccattac ggtcaattat tcacaacagt taccattggc acaaggactt gtatttgaat 1560 aaaagtttta tgtgaataaa ttttctgaaa ttcaccaact c 1601 <210> 33 <211> 1475 <212> DNA <213> Chironomus riparius <400> 33 tggaaattaa taatttaact agtggagtat tagctggtgg taaaaatgat aatatgttat 60 ataattcaaa agtaaatttt catccatcga catcctctgg tgtattttct gatataaata 120 aacaagaaga gttaccaggg attacaccac caccagaaaa tgataaaaat aataattctt 180 ttaatatggt tattattatt atattaataa ttttagttat tttaattata ataggtttaa 240 ttttatatgg attatttggt aaaagagata ctaataataa atataaatat agaacaaatg 300 aaattgttac tatgctttaa gttgaaatat taatataata ttaaatgagg attttagatt 360 taaatacggg atatttttta tctaatatta attatatata tataaataat ataaataaaa 420 atataaatca tcaagatata aaaatattat taaatcattt aaaaaaagat agtttaatta 480 ttagtttgtt tactacatat aataataaaa tgtatcatca taaaatgtta ccattagaaa 540 tattaaatga taattatgaa attaaaatta attaataatt tcttcagtat ctaaattttc 600 ttctaatata atatcttcat ttattatatt atttatttta tttttagtaa aaattctatt 660 tattattttt aatatattat cttcatttat taaatatttt ttaatatcat taaataatct 720 atttatataa atttcaattg ctagtctata attttctggt atatatatat catttgaatc 780 taatatccat aatttatctg ttactttttt taatgattca aaatcaaaaa atatattatc 840 taatattttt ttatctgata atatagatga tgttatataa atatatttaa atctatctcc 900 aggtgatatt ttttctttat gtcttggatt attattaaat aattctactt tatttttaat 960 ataatgtgta gatgattttg tatttttatt atatatcata gttaatatat aatcatttat 1020 atttaaatta ttataatcag ttaatattat attaattatt ttatttattt catttaataa 1080 tatattatca aatgtatcaa tattatctgt taaatccata ttttctttta taaatgtaat 1140 taatttgtat atagaatttt tatgaaattg agcataatct cttctaatta cagaaatacc 1200 tttattatag ttttttatat ataaattaat attagtatta tgtaatacta tacttggttc 1260 taatgatttt acagtatatt tttttttcta acataattaa atcaaaataa atattttcaa 1320 attcaaataa aattttatta taaaatattt cattatttaa tatattaaac aactcaaaac 1380 ctattttact agcatcttta atatttattt tttcttttat attatttaat gattctattt 1440 ttatcatatt agaatcagta tcaccatata caact 1475 <210> 34 <211> 1695 <212> DNA <213> Chironomus riparius <400> 34 tctccgactc agtcaaaatt gaacgaaccc catttgaaaa ttgattgaat ttttttgagg 60 aaaaaaataa agtttaatca aagatcgtga tggcatcgag aattttaaag cttacgagta 120 ctcttcgagc attgccaaaa gcaggaacaa tccagcgatt aaagtcaact aacgccacag 180 attatgcacg agcactcgta aatgtcccac caacaacagt ttcaacatta gataatggat 240 taagagttgc aactgaagac agcggttctg caacagcgac tgttggtctg tggatcgatg 300 ccggatctcg ttatgaaaat gccaacaata atggtgttgc acactttttg gaacatatgg 360 cattcaaagg aaccgctaag cgctcacaaa ccgacttaga attagaagtt gagaacatgg 420 gagcacatct taatgcctat acaagtcgcg agcagacagt cttctatgct aaatgccttt 480 caaaagatat cccaaaagct gtagaaattc tcggtgacat catccaaaac tcaaaacttg 540 gagagtcaga aatcgaacgt gaacgaagtg taattttgcg tgagatgcaa gaagttgaga 600 gcaatcttca agaagttgtt ttcgaccatc ttcatgccac cgcttatcaa ggaacacctt 660 tgggaaatac aattcttgga ccaacaaaga atatcaaatc aattacacgt caagacttga 720 aggattacat tgatgctcat tatcatacac caagaattgt cttggcagca tcaggtggtg 780 tccaacatga tgatttagtc aaattagcat caaaggaatt gggaaaattg aagaatactt 840 tcgacggaaa ggcaccaaca cttgaaccat gccgttttac aggctcagaa gttcgtgttc 900 gtgacgattc aattccatta gctcatgttg caattgccgt cgaaggatgt ggctggactg 960 atcaagataa tgttccatta atggttgcta atactcttat cggctcatgg gatcgctcac 1020 aaggtggtgg agtcaacaat gcatcacaat tggctaaagc ttcagctgaa gacagcttgt 1080 gccacagctt ccagtcattc aacacatgct acaaagatac cggcttgtgg ggtatttact 1140 ttgtatgtga tccaatgtct tgcgaagata tgctcttcaa tgttcaaagc gaatggatgc 1200 gtctgtgcac aatggtcacc gaacatgatg ttgagcgtgc aaagaacttg ttaaagacaa 1260 acatgctcct tcaactcgac ggaaccacac caatctgtga agatattgga cgtcaaatgt 1320 tgtgctataa ccgtagaatt cctcttcatg agctcgagaa acgtattgat aatgttaccg 1380 ccgataacat cagagacgtg tgcatgaaat acatttatga tcgttgtcca gccgtcgcag 1440 ctgtcggtcc agttgagaac ttaccagact acgtgcgtat ccgatcaagc atgttctggc 1500 tcagagttta aatttgttaa ttttaaacaa acaaacaagt aagaataata aaaaattgag 1560 ctatacactt aaactagcga aaaatgagag taaaattcaa atcaagcaag ttgttaattc 1620 aaagaaaata aattctgtat gatgtaagtc gataacaaat taataaaaaa tgaaaaatta 1680 ttcaaaacga ataaa 1695 <210> 35 <211> 725 <212> DNA <213> Chironomus riparius <400> 35 cacaaaaaca caaataagtt cacaataatt aattaaattt aatagtaatt aagctgttta 60 aagacacaaa aacacaatca aaatgaggat tgagacttgc tatttctgtt ctagcaaggt 120 ttatccagga catggaatgt tattcgtcag aaatgactgc aaacaattca gattttgccg 180 caagaagtgc aggaaggcat tccagcgaaa gaagaatcca agaaaggcaa aatggactaa 240 agcataccgt aaattagccg gaaaagaatt aacaatagat ccagcatttg aatttgagaa 300 gaaacgaaat gctccagtca aatacgatcg tgaattatgg acgaaaacag tcagtgccat 360 cagaaagatc aatgaaatca aagagagacg agagaagcac tttgtcatgg aacgattacg 420 aacaggcaca caacgtgaaa ttcatatgga tattcgtgat gtacagaaga atatagcact 480 gatccgagca ccagaaggca tcagaaagaa ggatacagag aaattggatg tgacaatgga 540 ggatgaggaa gaagagattg tttacatgcc agctaaggag atggagaaga agttggctgc 600 tggaattatg gatgtagaaa tgcagaaaat aaagaattaa atttactatt aagcttaatt 660 aatgtgaaaa aatgttgaaa taaaagaaat atctgaatta ttttaattac gtgaaaaact 720 cgaag 725 <210> 36 <211> 975 <212> DNA <213> Chironomus riparius <400> 36 gaacgattaa gtcatgattg gtaaaagttt tattcttgtt ttcgcctttg cggcaattgt 60 ttgtgctgat agccgtatca attttattta ttatcatgat ggcacaacaa ctgaatattc 120 tacagatacg cttccacaag tcctttctca tccatcattc cgacgaactg ctgaaactgt 180 tgcttttcat tatggagctg gtcagaatct tggatcttct gaggtttcag atattattaa 240 ctcttacata agcaacgctc gatacaatct tattgtcgtt cattacttta gcatggaatc 300 tattcacact gataacgcag tcgctctcag tgacagtctc gctacagcct tcactcgttt 360 ctttgatacc ggctataact caggctttat gaatttcgtt gcacatggac ttggtgcaca 420 aatcatggct cgagcatctc gtttgattca aagccgctca aactttcgtc atgttattgg 480 acgtttgact gggttagatc cagcaagtat gggaccagtt gctacaattc aaatcggaag 540 attaagctca gcagatgcac aatttgttga aactattcac accgatggca cattgaatgg 600 agatcattta tcatttggac atgtctcgtt cttcccaaat ggtggagaaa accaaccatt 660 ctgcacaaat ccagttccac cattaagagc caattgtaac tctgtatttt cgcttacaat 720 gtgggctgag tcagtcagag cagtttcaca aattttcccg tctttacaat gtgacacatg 780 gccacgatat acagctggag tttgcaataa caatcatgtt gcacatttgg gacgaattac 840 tgataataat ttacgtggaa cctacttctt gaggaccaat gatgctccac catttaaccg 900 cgaaactcca ttcccataaa ttttattgag tatgaaaatt gttgatggaa atgaaataaa 960 attcttgaaa gtaac 975 <210> 37 <211> 648 <212> DNA <213> Chironomus riparius <400> 37 tttttttttt taattttttt aaatatattt caaataatat tctacagcac atttagtaaa 60 tgttactcat ttgtaattag cgttcaccat caagtgcctc aagttcgtca gcaacttgtt 120 tagcaactct gtgatattta gccataactg gacgataata gtaagcttga taatcacgac 180 gctcagccat ctttgcctta atttcagctt ccaacttacg aagtctcatc ttttgcattt 240 cctcattaat tacatgacat tgcttctcgt attcttcctg gggtgagtta tggatatatc 300 gggaaatgaa tcttgaaatt ggatgtctgt gatattccca atgatttggc tcatagcctt 360 caggtgtttc tgtcaattga gcaggtccaa tgaagacatt agagtagaag atgatgccag 420 ttacaggaat aacacctagc atgacaaaga aatgaaacgt atccttgaat ttgtgccatt 480 gcattcgtgt tgggatcatt ggaaacacac gtggaccatg tgcactcatg ttacgagcag 540 cattctgaat gatactagat gctgctggtg acctcaaaag gcttgaaagc cgggtaatat 600 tggataattt tgaaattatt ggcatttttc aatatttcac gcactcgt 648 <210> 38 <211> 521 <212> DNA <213> Chironomus riparius <400> 38 acgagtgcgt aggaactttt ccaggcagaa tgaaattctt aattttggct ctttgcgttg 60 ccgcagcttc agcagctgtt gttccattat cagctgatca agcttcactt gtcaaatctt 120 catgggctca agttaaacat agtgaagttg atattcttgc ttccatcttc acagccaatc 180 cagacatcca agctcgtttc cctcaatttg ctggcaagga catcgactca atcaaggaca 240 ctggtgcatt cgccacacat gctggtagaa ttgttggttt cttctctgaa atcgttggac 300 ttattggaaa tcaatcaaat gtcccagcac tttacactct taccgaacaa cttgctacaa 360 gccataaagc acgtggaatc tcaacagctc aattcggtga attccgtgct tcacttgtct 420 catacctctc aaaccatgtc tcatggggtg aaaatgttgc tgctgcttgg aacgttgctc 480 ttgacaactt cttcttcgtt cttacctcaa actactaaat a 521 <210> 39 <211> 2593 <212> DNA <213> Chironomus riparius <400> 39 ttttttttta gtttatgatg aatctataac atctttagtt tttcgatttt tattctcctt 60 tttcagtatt tctaaccaaa acttatagct atgtttcatt ctttgatgag ttgtacaaaa 120 aatcataaat tattcaataa tatgacttca tgatatgaga atttaggaaa acaaaagtag 180 taattataat taatatctta aagaatttgg tttaaagcaa aaacaaaatg gaaaaagcgc 240 ttatgaaatt taagtgaatt aaggaattta aagttactca tgtttgtatg tcaaattctg 300 ttgatcgatt gatttaatca acttcttcca tatgagaagc atcttcggtg tcttcgacta 360 aaagtggcat atccgacgat gtggcagcag gtgtttcctc aacagtcatt gtatcatctt 420 catcgatacc caatccaagt ttaatcatgc gataaatgcg agcagcatga acttgtggtt 480 catccaatga gaatccagag ctcaataaag atgtctcgta gagtaaaatg cacaaatctt 540 taacagcctt atcattcttg tcagcttcag ccttttgacg taaagtttca atgattgaat 600 ggtctggatt aatttcaaga tgtttctttc cagccatata acccattgtg gttgtatcac 660 gcaatgcttg tgccttcata atacgttcca tatttgcaga ccatccatat tgagatgtaa 720 caatacaaca tggagattcg actagacgat ttgaaacaat aactttttca actttgctgt 780 ccaagactga tttcattact ttgcagagat tttcaaactt ggttttgtcc tcttcgcgct 840 tcttcttttc ttcgtcgtct tctggcaatt ctaagccttc ctttgtgaca caaacaagtt 900 gcttgccttg atattctttc aattgttgaa tgacatattc atcaattggt tcagtcatgt 960 aaacaacttc atatccacgt ttcttgacac gttcaacgaa agcagagttg ctgacttgtt 1020 ccttactttc gccagtaatg aaatagattg atttttggtt ttccttcatg cgtgaaacat 1080 attcgcctaa tgagcacatt tcgtcaccgc ttgcagatgt ttggaatctc aataaatcag 1140 ccaatttagc acggtttgta gagtcttcat gaacacccaa tttcaagttc ttgctaaatt 1200 gagcatagaa tttcttaaaa ccttctttgt cttcaatgag ttcttcgaac aattctaaac 1260 atttcttgac aagattctta cgaataactt ttaaaatctt gttttgttgc aacatttcac 1320 gagaaatgtt taatggtaaa tcttcagaat caacgacacc cttcataaag ttcaaataat 1380 caggaatgag atcttcacaa ttgtccataa taaagacacg acgaacatac aatttaatgt 1440 tattcttctt ctttttagct tcgaataaat cgaatgggat acgacgagga acaaaaagca 1500 atgcacggaa ttcaagttgt ccttcaacgc tgaaatgctt aacagctaaa tgatcttccc 1560 agtcgtttgt caagctcttg taaaattcac catattcttc agtagtgata tcatctggat 1620 tacgtgtcca aattggctta gttttgttaa gttcttcatc ttctgtgtac ttaactttaa 1680 cggttttttc tttttgtcag cgctcttatc agcatcctcg tcatcaccaa catcttcgat 1740 tttaggctca tcaccttctt tcttttcttc ttttggttcc tcaggttcag cttcgtcgtc 1800 gcttacttcc ttttcgcgtt ctttttcaac gaccaacttg attggatatc caataaattg 1860 agaatgttta gtgacaatag acttgatctt tgattcttct aaatattcag tttgatcttc 1920 tttaatgtgc aatacaatct ttgtaccacg acctaaatct tcaccgtcat caacagcaat 1980 tgtaaagcta ccaccagcag atgactccca aacgtattgt tcatcatcat tattctttga 2040 gtatacagta actttatcgg ctaccaagta tgctgaatag aaaccaacac cgaattgacc 2100 aatcatagaa atgtcagcac cagcttggag agcttccata aaagccttgg ttccagaacg 2160 tgcaattgtt ccaagattat tgactaaatc agatttcgtc ataccaatac cagtatcgat 2220 gagtgtcaag gtgcgagcct ccttatttgg aataatcttg atgtacaatt ccttgccaga 2280 gtctaatttt gatggatcag taagtgactc ataacggatc ttatccaaag catcagatga 2340 atttgagatc aattcacgca agaaaatttc cttgtttgaa taaaaggtgt tgatgatcaa 2400 tgacataagc tgagcaattt cagcttggaa ctggaaaact tcagcatcag gcatctttct 2460 tgatttattt agacttcaaa tactatcaat atatccttaa ttaattgttt caaatagctt 2520 ttaagcactt ttttcagtaa tttccttaat ttcacaaaaa aaaatttttg ctttattcac 2580 ttctgtcgag cgt 2593 <210> 40 <211> 695 <212> DNA <213> Chironomus riparius <400> 40 acgagtgcgt aaaaaatagc accaaaagca aaaggaaaat tcggaaaaat cgattaaaat 60 gaacgcaaag acaacacttc gttttttaca agctacacgc caattctcaa gatcatcagc 120 agcatgtaag gatcaagttc atcctggata taacaaaatt aagtcaatcc aggaccaatt 180 tcagaaacca gatggacttc cagtacattt aaaaggaggt gcaatggata aggttctcta 240 tggtacaaca atcgttgcct gtgtttttgg agtgcttgga ttcggtcaag tcctctacga 300 tcttggattt gcaaagaaga attaagttat tcctcaatcc caagtcaaat cattaaataa 360 gaataagtag tcgacttatc ttgtcgatgc agtgctgctg ggattttaca aacacataaa 420 agtatataaa ataagcataa tgtgggatac ttaagcattt aaaaacgaaa tttccttctt 480 aaaaagcatt tatgcgtcat taaatcaact tatctacctt aaattccgca tttattaagt 540 aatattgtga acggatgaag atgtaaagtt ttattttttt tatgtcacag aaataatata 600 ttaaagaccc aaaataaaat tgagcaagca ttggctgaga cgagagtttc caaaattatg 660 aaaaattgaa aaagaaaaaa aaaaaaaaaa aaaaa 695 <210> 41 <211> 1567 <212> DNA <213> Chironomus riparius <400> 41 acgagtgcgt gtaaaacgca cgaaaatgat tctcgaaatc atattgattt ttattggatt 60 tatattgtgg atgtgcagtg attatatttt tcatgatccg ctcaaaaatt tcagcaaaac 120 ttcaattcct ctgttaaaga attcatttaa tattgtcaga atgagtccag tcgaaatttt 180 tcaatttgtt cgaaacggcg ctattaaata tcaaaagtcc tatcaacata gtttttttgg 240 atgctttttt gtatacaaca ttataagagc taaagatgct gagaaaattt taagctcatc 300 aaagttattg aagaaaggac gagcttacca atatttgcat ccatttttaa agactggtct 360 attgactagt gaaggagcaa aatggcatca gaggcgaaga atgttaactc ctgctttcca 420 ctttaatatt ttaaaggaat tttgtgaaat tttcagagaa gaaagtgaaa aattggttca 480 gcagttaaaa tctttaacag gaaaagaaat tgacataatt ccgatttcca ctcaatttac 540 cctcaacact gtttgtgaat ctgcaatggg aattaagctt tctgatctgg gaaatgatgg 600 aaagacttat agaaacaaca tctatgaaat tggtagttta ttagtttcta gaatgacaaa 660 accatggctt tttaatgatt ttgcgtatag actttttggt tctaagaaaa tcttagataa 720 agttcttaag cctattcatg aatttactca gtcaatcatt agcaaacgta gaattgattt 780 tcttattaaa agaagcatta ataatgaaca accaattcaa gaaactgatg acgaaaatat 840 ttatttgaga tcaaaaaaga aaagagttgc aatgatggat actttattac aagcacaatc 900 tgaaggctta atagacgatg aaggaattat agaagaaaca gacacattta cttttgaagg 960 acacgatact acatcagctg gaatgacatt tacattattg ctgctagcac atcatcaaga 1020 aattcaggag aaaatatttc aagaaattca agaagtgacc gatggaaggg atgatcttac 1080 cacagatgat ttaaataaga tgaattacat ggaaagagcg ctaaaagaat ctttgagagt 1140 ttatccacca gttccttaca tttcaagaac ctttacaaaa gactttgaac atgatggcgc 1200 tttataccca aaaggatctt ttgttcaaat tatgatctat gacattcatc gtgacccaga 1260 atattttcca gatccagaaa aatttgatcc tgatcgcttt cttcctgaaa attgtataaa 1320 tagacacaac tacgcgtttg ttgcattctc agccggaatg agaaactgca tcggccagcg 1380 ctttgcaatg ttagagttaa aaatcatgtt agcacatgtt atcaaaaact ttaaagtttt 1440 accagtcaca aaaagagagg aacttgtttt caaagctgat ttggttttaa gaacagttga 1500 tcccgtgagg atgaaatttg aattgagaaa atgaataaaa tgctatcaaa aataaaaaat 1560 tctctac 1567 <210> 42 <211> 749 <212> DNA <213> Chironomus riparius <400> 42 acgagtgcgt ggtctgttca acacgcatca ctagtgcctt tcttgttaat tgtgaaaata 60 ttataaaatg gctagaattc cacaactaaa gattttgacg gaggaacaag ccaagctcgt 120 tcaaactgac tgggattata tcagaactca agctgtagaa atcctttaca atttctttga 180 aaaatatcca ggaaatatga aacattttaa agccttcgct ggcaaagact tagatgacct 240 taaggaaacc cctgaatttg ctgctcatgc tgaaaaaatt cttggagttt ttggtcaagt 300 cattgacctc ttgggcaagg atattgatgg aattaagaag attctcaacg aaatggggaa 360 taatcataga gctagaggca cttccagatt tgctttcatg gaattcagag aagcattgat 420 ggaatttttg cgctttaatg ttggtagttc ttacgatgga atgcaggcat ggactgatgc 480 tatcgattgc atgtatcaca tcatctacaa caatcttgaa ggaaatccag tttaaaataa 540 tatttattct aatcttaaat ttattttgtt ttgtttgcaa tcatcgagtg ccatatctgt 600 tcatgctatc aaaggtcttg caaaattctt tgcatggata gaacaaaaat tcaattgctg 660 gttagaaata tgacaaaaat tacacacaca aaatgtatta ccttaattat gtaaatgtat 720 gaataataat aaaaataata aataaaatt 749 <210> 43 <211> 1036 <212> DNA <213> Chironomus riparius <400> 43 acgagtgcgt taaatattta aaatttgtta atttataaac aaaagttgtc aaagttaaac 60 aaaaaaaata tctcaacaaa catgaagttt ttatatatta tctcgatgat tttttgtgca 120 gtcaatggat tcaggtttaa agatgttatc tcagatcaca tagacgtatc gccacacaac 180 gaatgcgcta atcaaccaaa tggtatgaga cttccacatg ctgattgtaa tcgattcata 240 ctttgtgatt cacaagctgg actggttctt tcatgcagag cagcagagcc gaattttgat 300 agatgcagtc agagatgtgt atatgatccg ttagtgtgca cgataactga ttgtaatggc 360 ggcggtgggg aagtaacatc gaccacagta acaagtatac ctccagtaac aacaacaaca 420 acaaggttta ctcctccaac agttcaagct gtatgcccaa ttccttgtac ttcagaaaac 480 atgcctacat cctgtgactg tatagaacga tggatatgcg ttggtgtaac ctgtcaatgc 540 aacgctaaca tcccgtgtcc tatgtcaaat gaagattgta tggcactacc agaatgtggc 600 ggtgattgtc ctgaacgatg tgtttgtgaa actcctgaaa attgtcaatg cgacactgaa 660 cttcaatgtc cattcgcaga gactgaatgt gttgcaagat gcggttgtgg aactgattgt 720 aactgcgaaa caccaacaac gtgtgcctgc aatgatgtat gcaattgtcc acaaccacca 780 aatgtatgtc catttgctcc cgttgactgt attgcacgtt gcggatgtaa cgaaagatgt 840 atctgtaatg gaaatgtttg cacatgtgat actgaaactc actgtccagc aaatgttcct 900 aggattttta tgaataagaa ttaaatgcgg atattaagga tgatttggat attttttaaa 960 aatggttcag gaattattat tttagttttt actattaatt tgaattatat ttaaataata 1020 aaatatattt taaata 1036 <210> 44 <211> 877 <212> DNA <213> Chironomus riparius <400> 44 gtgcgtgctt cacacgtttt tgatcaaaat gaatcaaaca atttcaaaaa acgcaagttc 60 gtagccgctg gagtcttcaa ggctgaattg aacgaattcc ttactcgtga acttgctgaa 120 gatggatatt caggtgttga agtacgtgta acaccatcac gtaccgaaat catcattatg 180 gctactagaa cacaaaatgt tttgggagaa aaaggtcgtc gtatccgtga attaacagcc 240 gtcgtacaga aaagattcag cttcccacaa ggagccgttg aactttatgc tgaaaaagta 300 gcaaccagag gactttgcgc aattgcccaa gcagaatcac tccgttacaa acttattgga 360 ggtttggcag ttcgtcgtgc atgttatggt gtcttgagat tcattatgga atcaggagct 420 aagggatgtg aagtcgttgt ctctggaaaa cttagaggtc agcgtgcaaa atcaatgaaa 480 ttcgttgatg gattgatgat tcattcagga gatccatgca atgattacgt cgacacagcc 540 acaagacacg tactcttgag acaaggagtt ttgggaatta aagttaagat tatgcttcca 600 tgggatccat caggcaaaat cggtccaaaa cgtccattac cagacaatgt atctgttgtt 660 gagcctaagg atgaaattac atatttgaaa cccgacacag aatacaaagc aaaaccattg 720 tacatggctg gtgctgaaga accacatatt gatgtcgcca agtaaatccg tttgtgaatg 780 aatgaggagc actgataaac atctgaaata tttcaagtgc tgtaaattaa ataagaaaag 840 atcgtcaaat aaaaatgaag taaatctatt ccaaaac 877 <210> 45 <211> 777 <212> DNA <213> Chironomus riparius <400> 45 acgagtgcgt ctcgagtttt tttttttttt tttttttaat cattcataaa cttttcaatt 60 tatttctgcc tgttgaactg atatttttaa tgtaagaaaa tttacaatag ataatatgag 120 ttaatgtaac aaaaaaaatg ttttactgcc tattgcaaat actttcgcgg ttcatattgt 180 ccatttttga ctgaagttgc cgtacatcag atcgaattgc ttagtgaact ggatttccat 240 caaggctgct gaacacgata gcgtacattt catcaaatgc cttgttccat gcggcaccag 300 tggcatcaga ccaggctcca gagtgttctt taagatattt agcaactgat gacttgaact 360 tttcgaattg ttcctttgtt gcaccacgac ttctgtgggt ttgtcctaat tcattaagga 420 gagtcttaat ggctggtgtg ttagcttcat tgccaaccaa ttcgatgtat tttgacactg 480 cagcgacaat tctgtcggca tgctttgtaa agtcatcatt ggatttaatt tcttctaagt 540 tctttccaac gaatgctggg aatttagctt ggatgtctgg attttccttg aaaattgcat 600 agagaattcc atcttcttgt cccttaactt tatcccatga acccttcacg agcttagctt 660 catcagcatt taaggtggca tatgcaccag caactagtac ggcagcaaga ataaggaact 720 tcattttaac ttagataaac tcagacgtct gttcacgagt aaattcaacg cactcgt 777 <210> 46 <211> 634 <212> DNA <213> Chironomus riparius <400> 46 aatttatgga ttaataaaat ctatgattta ttaaatttga gtttatggca tatacaatat 60 tcactattta aacagcaact atttcctcaa aataacaatt tacaagattt tataaaatta 120 atttaataat gatattgatg tccatgatta gcaaatactg gtgctggttg ataaactttt 180 gaaattgtag ctggttggta gactttggtg acaactggtt gtgcttgagc aatgattttg 240 tggtcaactg gttcacgttg tacattagct tggaatccat gatgatcatc agctgtgtat 300 gaaacaacac gtctgtatcc atcagcatca attaatgagt attgtcctga gatagcacca 360 tccttggcta cttcttgttg acgcttgata tctcctgtgt gttggtcatg aacttcatag 420 ttaaattcgt aattggctgg ttcttcatga actgcttgtt taacataagc agcttgctta 480 atgacagctg gctgaacata agttgttggt tgataagcag ctggttgatg ataatagtta 540 gaattgtatc caagatctac aactccggct gagacgactg ataaaattgt gaatgataag 600 actaatttga atgccataat gatttttttg tttg 634 <210> 47 <211> 556 <212> DNA <213> Chironomus riparius <400> 47 agcagtgaaa cagccaaaag cggctgctcc agccaaaaaa gaaaagaaaa cagaaaaagc 60 tgctccaaaa ccaaaagaag cagttgcagt aacacctaga cctttcaaac gttatggacg 120 tctttggtca aaggctgtat ttactggatt caaacgtgga ttgagaaatc aacacgaaaa 180 tcatgccttg cttaaggttg aaggatcacg tagccgtaaa acttccttgt tctatgttgg 240 caaacgctgt gcatttgtat tcaaaggaaa atcgaagaaa tcatccccaa ccaattcaaa 300 gcataagtca cgcatacgtg caatttgggg aaaagttact agactccacg gtaacaatgg 360 atgcgttcgt gctcgtttca agacaaattt gccagctgat gccatcggtc accgtgtcag 420 aattatgctt tatccatcaa ggatctaaac atacttcctt aaataaatgg atttaagaaa 480 tgaaacaaca aatttgaata aaatatccat tgaattcagt tccaacaaaa aaaaaaaaaa 540 annnnnaaaa aaaaaa 556 <210> 48 <211> 685 <212> DNA <213> Chironomus riparius <400> 48 aaaggagtac tcaaggagta tgagatcatt ggcagaaaat tgccatccga aaaggatcca 60 aatcctccat tattcaaaat gaatatcttc gctccagatc atattgtagc caagagtcgt 120 ttctggtatt tcttacgtca attgcgtaaa ttcaagaagg caacaggtga aatcgtcagc 180 gttaagcgca agtacgagaa gactccattg agaattaaga atttcggcat ttggcttcgt 240 tatgattcac gttcaggaac acacaatatg taccgtgaat atcgtgactt gactgtcggt 300 ggtgcagtta cacagtgcta ctcagacatg gcatcacgtc atcgtgctcg cgctcattca 360 atccaagtca tcaaaattga aaaggtagct gccatcaaga cacgccgtac gctcatcaag 420 caattccacg attcaaagat caggttccca cttgtacaaa gatatcacca caagagatac 480 cgacaattgt tctctattac tcgcccaacc acccacttca attaaactta tgtttattgc 540 tcgagtgtac taactgtaat aaagtggaaa tcggttaaat aatgattgtc caggcgatga 600 aaccatttta aatctatggt ttgttgaaga attgatgtga ataaatatga taaaaaagaa 660 tttttccatc tataaaaaaa aaaaa 685 <210> 49 <211> 1033 <212> DNA <213> Chironomus riparius <400> 49 ggtattcaag caaaaagaag gctttcacaa aggccagcaa gaaatggaca gacgatttgg 60 gcaagaagac aattgaagat aacttcaaga aaatgattcg ttattgcaaa ttcgttcgtg 120 tcattgtcca ttctcaaatc cgtttgatca agcaaggtca aaagaaggct cacatcatgg 180 aaatccaatt gaatggagga accattgaag acaaagtcaa ctgggtcaag gaacatttag 240 aaaagacaat tccagtctca aatgtctttg gacaagacga aatgattgat tgcattgccg 300 tcacaaaggg taaaggtttc aagggtgtca ctagccgttg gcacacaaag aaattgcctc 360 gtaagacgca caaaggttta cgtaaggttg cttgtattgg agcttggcat ccaagtcgtg 420 tagcctttac tgtagctcgt gctggtcaga aaggttatca tcatcgtact gaaatcaaca 480 agaaaatcta ccgtattggt gctggaatcc atacaaagga cggaaaggtc gttaagaaca 540 acgcagcaac tgaatatgat gtcactgaca agtcagtcac tccaattggt ggtttcccat 600 tctatggtga agtaagaaac gactttgtca tgcttaaggg aagctgtgcc ggtgccaaga 660 agcgcgtcat tacattgaga aagtcattgt tggtccatac atctcgtatt tccaatgaat 720 ctatcaatct taagttcatc gatactagct ccaaaatggg tcatggacgt ttccaaacat 780 ctgccgacaa gtcccaattc atgggtgttc ttaagaagga tcgtcttcgt gaagaaaagg 840 ccacacttgc tgcaaatgct gcaaagaagt aaactgaacc agaaaactga aaatatgtta 900 aataaaagga tttttcgtag tctattgtca tctgaaatat acgaaaatga ttaaaataaa 960 ggattaattc ttcaattacc ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa naaaaaaaaa 1020 aanaaaaaaa aaa 1033 <210> 50 <211> 559 <212> DNA <213> Chironomus riparius <400> 50 aatttttatt taaaaagatg acgactgcaa ccattcgtac aagaaagttt atgacaaatc 60 gtctcttatg tcgcaaacaa atggtatgcg atgttcttca tccaggatta ccatcaattg 120 caaagaagga agttcgcgaa aaattggcta cgatgtacaa agttacacct gatgttgtct 180 ttgtttttgg cttcaaaacc aactttggag gcagcaaatc aacaggattt gcattaatct 240 acgacacatt agattttgca aagaaatttg aaccaaaaca cagattggct cgtcatggac 300 tttatgaagt caagaagatt acacgcaaac aacgtaaaga acgtaagaac agaatgaaga 360 aagtccgtgg taccaagaaa gcaaaggttg gccaattaac aaagaagtaa attgatggat 420 atatttgaat tatacttctt tccattaaga tacttttcca tcaaattgtt cttgcttatt 480 gtacattctc gtgaattgtg aaaataaaat aaatgaataa agcatgaaga tgtgcgataa 540 tcagaattaa tataaaaaa 559 <210> 51 <211> 578 <212> DNA <213> Chironomus riparius <400> 51 agcgtgaaaa aagtcggaat ctttaattct gcttaaattt cttgcaaaaa tgggtttcgg 60 agactatcca gcagaatata atccaaaggt tcatggtgtt tatgatccag cacgcttcta 120 tggcaaacct gacactccat tgagccaggt caaagttagc gaattaggat catggttcgg 180 aagacgtaac aaatcaccaa gtgctgttgc tgctgcttgc tcacgagcat ggtggagatg 240 gcaacacaag tatatgcagc caaaacgcag tacaatggct ccatatttcc aacttgttgt 300 tggatctatg attttcttct atgccattaa ttacggaaag atgaaggctc acagaaacta 360 caaataccat taaacaagac tttccaaatt cttcaacgtt tgcaaagcat acagctcttg 420 atgatacttt agaaaatcaa tgaaatttca acaaaaatcc attaaatacc gcttacattt 480 cgaaaaattt aaaagttatg tcttgatgat tcaagagaag taagttcact taatatagaa 540 cgaataaaaa tcgattaaaa attggatctg tcgagcgt 578 <210> 52 <211> 2860 <212> DNA <213> Chironomus riparius <400> 52 aggctttcga tgatgcaatc gctgaattgg acacactcaa tgaggactca tataaagatt 60 caactcttat tatgcagctt ttgagagaca acctcacatt atggacatca gacacgcaag 120 gtgatggaga tgaaccagca gtgggagatg ataactaacc aaactaaatt gtcaaaaatt 180 cgagatgata aaaacaacta tcaactaaaa caaatttaaa taagataatt tttttgcgtc 240 gcagagaaga agaagaaaga aagaaatcgt ttaatgcagt tatatataat ttatattttt 300 tttttttcct ttaattgagt caactctgaa agaaaacatt ttatataaag atgaagaaag 360 aagaaaatga aagatacgcg cagtttttgt aaagtttaaa aatgtattcc ttattattct 420 tacatttcat tcatttatct cctttttttg tttaatttat tatattttcc atgcgctttt 480 atcaggatat ttggaaaatc agtcttcgaa aagcactttt ttaaaaaaat aaacaaaaca 540 aagaaaaaaa gcttagctta atttaagctc caccaaaact tgagagaaac ttaaaagaga 600 aaaaagtttt tcgctaaaaa ctgcaacttt atcaaattta ttaatttttt aacatccaat 660 cgattgatag tcatttattt tctgttgaac attttatttg cttttggatt tttgtcattt 720 gaaaaataaa aaaatttaca ttacacaacc aatatattaa atatattaaa tacataaatg 780 tgcccgaatg aagctctatt tatttagtca tcgttggtcc tttctttctt tcgcttttgt 840 atgtatgttg accatttttt taagttcctc taataataac cgctattttt taaacaatta 900 aaatatttga cggttatttt ttcaaatatt ttttgatcgt tattttttca tcatttaatt 960 tcaacaattc tttatttcat catttccctt tattagcttc caaccttttt gttttttctt 1020 cttcttatcc gcttaaattg ttaataaaaa ttttgttatt atatctaata cataaaaaaa 1080 gaaaaaaaat ataataagaa catcaccatc tctgccgaaa gaacagaaca aaaaaaagta 1140 aattaataag aattttgcgg aatacaaaag gaacagcaac aaccgctgaa acaaaagaaa 1200 ttgatatttt ttcaagcagc atgaattatg cttcaaaaaa tccaaaaatt aaattaacat 1260 catatggaag aattattact ctttttcaaa caaaacaaga aaaaaactta aacgcataag 1320 attttaaaac ttaaaataga ttttgccatt tgaagcaaaa ttgatattat ggaacgtctc 1380 aataaagatg taagaaggat tgataaaaaa tgatgatgga taagataatt tccctttttc 1440 tatatacaag gctcataatg gctcgctgtt attaaatatg tttaattttt ttaaatacag 1500 aaaatatgtc tgttttgctg tattcgcttt tgcataactt aagcggaaac acacgaaaaa 1560 ttgaagtgga aaataaaatt ctcaaattcc tctaaaaaaa caacaaatgg gattttattt 1620 cttcatataa ttagaattaa caggatgtat ctctttttaa ccaaatcacc atcaaaattt 1680 cctttttgcc agattgaaga acttaatatt attatgagaa taaaaaataa tgaattaaaa 1740 atgataaagc agtgatggaa atgtgagtgt gaaacatgat ttattacatg tttaaaaatt 1800 aaaaaaaata atagacgata acattatttc ttgcaacttc taaatccttc cttcagaaat 1860 atcctaatac acacatacag gaatagattg ctgtattcgg attattgact ctttttgagg 1920 aattttacat catttttgtt gttgattaag gtcgaggaag ttaaatttaa gcatgagata 1980 agcagataaa tctgtggatg aaattaatct aatagattta aattgtaatt ctaagattta 2040 ataattaatc cttggattta aaataaatct gactggtttc aacttaaatt gattctaaag 2100 attgcagatc attcgatcct gaaaatcaat aatcaagttt atgtgacatt ttctgtaaag 2160 aatagatgcc caaattggca gataatctct cgaaaactga agaattaaga cttcctttaa 2220 gacaagcatt tttctttctt caaattgaca ctttctgcat ttttcaataa aactaaaacg 2280 ttaaagaaat tcaatattga aaaaacaaaa ctaataaaat aatgatataa tatgaaatgg 2340 aagtaccagt accgtaccaa agtgttatga atatcaattg aatgtaaaag catcctttca 2400 tataaaatat gtgtatgcca tcgttttcga aatgtctttt attggtgata tattgaatga 2460 catgagaaat tcgataaact atgaatttag taaaaccaca aacaaacaca aaacacacat 2520 gcaagacatt tcaatgcagt atatagtccc tattttcaca aatttctctc ttcttatatt 2580 tattaattaa taaaaaatcg tatattgaaa aaaaatgttt aattggaaat aatggaattg 2640 gatgatattg gaaatttgcg gatatctatt tgaatataat tctttgtcgc aacaaaaaaa 2700 aattattgta ttgtgttcct ttttcgtcga gtatttttcc ttcccttaaa aaaagaatat 2760 attaaaaaat aatgaaaaaa atgataaaag tatcattaaa aagtcatata acaatggatg 2820 taaatcgatg gctcaaaaaa atgcgaataa caaatttttt 2860 <210> 53 <211> 597 <212> DNA <213> Chironomus riparius <400> 53 acttcaagaa acatgttcat catacacgcc gtacattagt tccaggcaaa gtattgattc 60 ttttggctgg tcgtcataag ggcaaacgag ttgtattaat gaaggttttg caatccggat 120 tattgctcgt taatggacca ttctatttga atagctgtcc attacgtcgc atctcacaac 180 gttatgtgat ctgcacaaag actcgtgtca acttgaaggg tgtcactatt cctgaacaca 240 tcaatgatac atacttcaag cgcgctgata agaagaaggc acgcaagacc gaaggtgata 300 tctttgctaa acaagaagag aaatacacac catcagagca acgcaaaaag gatcaaattg 360 aagttgataa gcttgtacgc ggtgctatca agaagacaaa tcaaggattg ttggtcagca 420 aataccttaa atcatacttt gcattgagaa accatcaata tccacatcgc ttgcgtttct 480 aagctaattc tttatgattt aacttcattt tcctcccttt tttcgtgctg cgatgatgaa 540 aaatatgtaa caacagtttt gaaatgctga aataaatgga aaatttatta taacgga 597 <210> 54 <211> 538 <212> DNA <213> Chironomus riparius <400> 54 gaacacaaac gccattgcca attgctgcac attccaaaga ctgttttaca gtgtacatgt 60 taccaatgca ctcaaatgtg tagtcaacac atccaaattt atcgttcaag tacttgtcca 120 ttggaccttc aacttccttt ggattaataa agtcagtgac tcccatttca gcagcaattt 180 cacgtttggc atcatttaag tcaataccga tgacagtttt tgctccacga tgtttacatc 240 caagtacggt ggcaagtcca attcctccta aaccccaaac agcacagact gaatctggct 300 tgactgctgc tgtcttaact gaaccgccat agcctgttgg gaatccacaa ctcaataagc 360 aagcaatatc caatggtgca gctggattga ttttagtcaa attgaattcc ttcaagactg 420 tatattcaga gaatgacgaa acacccaaaa actgtaaaag ttgctttccc ttgcatgtga 480 ttctcgtggt ctcatcagcc atcaattgac gtgactgaga tccgcttgtg aattcgag 538 <210> 55 <211> 656 <212> DNA <213> Chironomus riparius <400> 55 aaattgaagt tacattcgat attgatgcta atggaatttt gaatgtcaca gctttggaaa 60 aatcaacaaa caaggaaaat aagatcacaa ttacaaacga taagggacgt ttgagcaagg 120 aagacattga acgtatggtc aatgaagctg aaaagtatcg taatgaggat gatctccaaa 180 aggacagaat tgctgcaaag aatggcttag aatcatactg ctttaacatg aaatcaacaa 240 tggaagatga aaagttaaag gataagatct cagaaactga caagaaagtt atcttggata 300 aatgtaatga taccattaag tggcttgatg ctaatcaatt gggcgaaaag gaagaatatg 360 aacatcgtca aaaggaattg gaaggaattt gcagtccaat tatcaccaaa ttatatcaat 420 cagctggtgg tgcagcaaat atgccaaact tcccaggagc aactccaaat ccttcaggag 480 cagctccaac cgctggatca ggttcaggcc caactattga agaagtcgat taaatattca 540 atttgcggaa ccaaatagct tgtttttgcc aacttgatac gcttcccatc atttattttc 600 tttcaaattt tcacttgaac ttacttaaag cttgaagtaa taaccaccca aaaaaa 656 <210> 56 <211> 3017 <212> DNA <213> Chironomus riparius <400> 56 taatttgcat cttagattta cgacacaaaa ccattaaaca tggaagactc gcattgcaaa 60 acatccgaag aagtgttgaa attcttcaac acaaatgatg aattaggatt aacaccagat 120 caagtcaaac aatatcaagc taaatatgga cctaatgaac ttccagcaga ggaaggcaaa 180 tcaatatggc aattagtgct cgaacagttt gacgatcttt tagttaaaat tttgttattg 240 gctgctatta tttcatttgt attagctctt tttgaagaac atgagggtat tgaagcattc 300 gtagaaccat ttgtaatttt acttatttta attgctaatg ctgtcgtcgg tgtgtggcaa 360 gaacgtaatg cagaatcagc tattgaggcc cttaaagaat acgagcccga aatgggtaag 420 gttgttcgtc aagacaaaag cggtgtccaa aagctccgcg ccaaggaaat tgttccaggt 480 gatatcgttg aggtgtcagt cggtgacaaa attccagctg atattcgttt aattaagatc 540 tactcaacca caattcgtat tgatcagtca atcttgactg gtgagtcagt atccgttatt 600 aagcacacag atgccgttcc agatccacgc gctgtcaatc aagacaaaaa gaatatcttg 660 ttttctggta ctaacgttgc tgctggtaaa gcacgtggta ttgttattgg tacaggatta 720 aacacagcta ttggtaaaat tcgtactgaa atgtcagaaa ctgaggaaat taagactcca 780 ttgcaacaaa agcttgacga attcggtgaa caattgtcaa aggtcatctc aatcatttgc 840 gttgctgtct gggctatcaa cattggacat tttaacgatc cagctcatgg tggttcatgg 900 attaagggag ctatctatta tttcaaaatt gccgttgctc ttgccgtcgc tgctattcca 960 gaaggtttgc cagctgtcat tacaacatgt ttggctttag gtacacgtcg tatggccaaa 1020 aagaatgcta ttgttcgttc attgccatcc gttgaaactc ttggttgcac atccgttatt 1080 tgctcagata agactggtac attgacaaca aatcaaatgt ctgttgcacg catgtttgtc 1140 tttgacaaga ttgaaggaaa tgacagtagc ttcacagaat tcgaaataac tggatcaaca 1200 tacgagccaa ttggtgatct cttcttgaaa ggaggtaaag taaaggcctc agactatgac 1260 attcttcagg aacttggaac aatttgcgtc atgtgtaacg actcagctat tgatttcaat 1320 gaagtcaaac aagcattcga aaaagttggt gaagccacag aaactgctct cattgtcctt 1380 gccgaaaaat tgaatccatt cgccgtacca aagactggtt tggaccgtcg ttcagctgcc 1440 attgttgttc gtcaagaagt tgaaaccaaa tggaagaaag aattcactct cgagttctca 1500 cgtgatcgta aatccatgtc ctcatactgc acaccattga aatcatcacg tctcggaacc 1560 ggaccaaaga tgttctgtaa gggtgcccca gaaggtgtct tggaacgttg tacacacgct 1620 cgtgttggaa ctagcaaagt cccacttacc tcagctctta aaaaccgcat cttggaactc 1680 actcgtcaat acggtaccgg acgtgataca ctccgttgct tggctttggc cacaggagac 1740 tcaccaatga aaccagaaga catggacttg aatgactcaa ataaattcta cacttatgaa 1800 gtcaatctta cattcgtcgg tgttgttggt atgcttgatc caccacgtaa ggaagttttc 1860 gattcaatcg ttcgttgtcg tgctgctggt attcgagtta ttgtcattac tggtgataac 1920 aaggctacag ctgaagctat ttgccgtcgt attggagttt tcggtgaaga tgaagacaca 1980 actggaaaat catactcagg acgtgaattc gatgacttac cacttcatga acaaaagcaa 2040 gccgttgcac gtgctcgtct cttctctcgt gtagaacctt ctcacaaatc aaagattgtt 2100 gaattcttac aaggcatgaa tgaaatctct gctatgactg gtgatggtgt caatgatgct 2160 ccagctttaa agaaggctga aattggtatt gctatgggtt caggtacagc tgtcgctaaa 2220 tctgcatcag aaatggtcct cgctgacgat aacttctcat ccattgtcgc tgccgttgag 2280 gaaggtcgtg ctatctacaa caacatgaaa caatttatcc gttatcttat ttcatcaaac 2340 attggtgaag tcgtctcaat tttcttgact gccgctcttg gcttaccaga agctctcatt 2400 cccgttcaat tgttgtgggt caatttggtt actgatggtc tcccagccac agctctcggt 2460 ttcaacccac ctgatttgga catcatgagc aagccaccac gtaaagctga tgaaggcttg 2520 atttctggat ggttgttctt cagatatatg agtataggaa tgtatgttgg tgctgcaaca 2580 gtcggaggtg ctacatggtg gttcatgtat agctctactg ggccagggct ctctttttgg 2640 caattatctc accatctcca atgcatagga ggaggagatg acttcaaggg agtcgattgc 2700 aaagttttct cagcaccaga accaatgaca atggctttgt cagttctcgt cacaattgaa 2760 atgttgaacg ccatgaacag cttgtcagaa aatcaatctc tcgttaaaat gccaccatgg 2820 acaaatatgt ggttggtcgg atcaatgtgc ttgtcattcg ctcttcattt cgtcatctta 2880 tacgttgagg ttctttctgg agtcttccaa gtcacaccat tgggtattga tgaatggttg 2940 gttgtaatga agttctcaat tccagtagtg ctgttggatg aagtgctcaa attagtagcc 3000 agacgaattt ctgatgg 3017 <210> 57 <211> 508 <212> DNA <213> Chironomus riparius <400> 57 gtacatctgg aaattatgca tcagttattg cccataatcc agatacaaag agaacaagag 60 tcaagcttcc atcaggtgca aagaaagttt tgccatcaag taatcgtgct atggttggaa 120 tcgtcgctgg tggtggacgt attgacaaac caattcttaa agctggacgt gcctatcaca 180 aatacaaggc aaagagaaac tgctggccaa aggttcgtgg tgttgctatg aatcccgttg 240 agcatcctca tggtggtggt aatcatcaac atattggtaa ggcttcaact gtcaaacgag 300 gaactccacc tggtcgcaaa gtcggtctta ttgctgctag acgtaccggt agaattcgtg 360 gtggtaaggg tgatgcagac aagaaagaaa aggagaagaa ataaattaag tcgagttatt 420 aagtgcgatc ttatcatctg tattgtcgct gtctattcca tcaagaagtt tttatgataa 480 acttacaaca ataaaataaa atatttga 508 <210> 58 <211> 730 <212> DNA <213> Chironomus riparius <400> 58 tttttttgaa ccgccaagct agagtagtct tagaagatcg aatgaaaaat tctctacaac 60 ttacggcact ccgcttgaat cctcgtcgtt tgcgaggggt tcaggctcta acatagcatt 120 atccatgatt gactgtaaac aatatcggag cgttcaaaaa cataatcgta attactagct 180 tcatatgata cgtacagaca catgggagaa tatgagaatg gatttaggtg agtacaaaaa 240 tattgcgaga atagatatgt gcccgtccat tataaccaat acaatgtgta taagagcctt 300 tagatgaact gttgttctgg cacggctacg accttagagg cgttcaggca taatccggca 360 aacgtagctt cataccaatg tccgctcgga caagtattgt gccattggtt tgtacctgtg 420 gttcctctcg tactgcacag gtatgccata tcaacagtat aaatgccacc agtagggtaa 480 aactaacctg tctcacgacg gtctaaaccc agctcacgtt cccttgaaag ggtgaacaat 540 cctacgcttt gtgaattttg cttcacaatg ataggaagag ccgacatcga aggatcaaaa 600 agccacgtcg ctatgaacgc ttggcggcca caagccagtt atccctgtgg taacttttct 660 gacacctctt gttaaaaact ctttaaagcc aaaaggatcg taaggccagg cattagccgt 720 cccgatacgt 730 <210> 59 <211> 573 <212> DNA <213> Chironomus riparius <400> 59 aaataaccca ttaggtatca atagaaattc agataaaatt ccatttcatc catatttttc 60 ttataaggat atctttggtt ttgtagttat attaatattt cttacatttc ttacattaat 120 tgcgccctat atattaggtg atccagataa ttttattcct gccaacccac tagtaacgcc 180 cccacacatt caaccagaat gatattttct ttttgcttat gctattcttc ggtcaattcc 240 taataaatta ggaggagtaa ttgctcttgt aatatctatt gcaattttat ttattttacc 300 ttttactaat aaatttactt tccaaagtac tcaattttac cctattaatc aaattttatt 360 ttgaattata actattacag ttgtattact aacatgaatt ggtgcacgac cagttgaaga 420 tccttacatt cttacagggc aacttttaac tgtaatttac tttttatatt ttattattaa 480 tccattaatt gctatttgat gagaaaattt atttaaataa tataattaat ttaatctaaa 540 taaattaaaa aaaaanaaaa aaaaaaaaaa aaa 573 <210> 60 <211> 929 <212> DNA <213> Chironomus riparius <400> 60 aaatttgact tgactggcat tccaccagca ccaagaggaa ttccacaaat tgaagtctca 60 ttcgaaattg atgctaacgg tattcttcaa gttagtgctg aagataaggg aacaggaaat 120 cgcgaaaaga ttgtcattac aaatgatcag aacagactta caccagaaga tattgagcgt 180 atgattaagg atgcagaaag atttgcagat gatgataaga aactcaaaga acgtgttgaa 240 gcacgtaatg aacttgaaag ctatgcatac agtctaaaga accaaattgg agataaggag 300 aagcttggag ctaaattgtc tgatgaagaa aaggcaaaaa tggaagaggc tattgacgag 360 aaaattaaat ggcttgaaga acatcaagac acagactcag aagattataa gaaacaaaag 420 aaagaacttg aggatattgt gcagccaatt atcgctaaat tataccaagg acaacctgga 480 caaggaccac caccgactgg cgatgaggaa gatctcaaag acgagttgta aatccgttca 540 tctcaatcct ttttctacta caacaaaaca taaacctctt aattcaaaat gatttaaatc 600 ctaagccgtt atcatttcct ttttttattt gttacattca tttataattt ttttcattcc 660 gagaattaat taattgttta aaaaattgat cttaaagtga tttagggaaa attttcatac 720 gtcgcataaa aaaaagaacc ttgtcctaat aatttattag taaaagaaaa caaaaataag 780 aaaccaatgc acatgactga tgaatgagcg aatgagtgcg tgatctttta agtttttttg 840 taaattctta agcattcgtt tctattatga aacaaattga ataaaaaata gaagttaatc 900 tagaaaaaaa caacaacatt aaaaaaaaa 929 <210> 61 <211> 876 <212> DNA <213> Chironomus riparius <400> 61 acgctcgaca cgaaaaggta atttttgcga tcttccttaa aattttaaat tggtttttgt 60 gctttaagta attggcatta tcattaatta ggctgttaag ggattaaatc aacattcgaa 120 aaagatggca agtcgaatgc tagcagcaaa atttctccaa cctacctctg ctccagctat 180 gcttctacgt gcagctagca ctggcgcaat gaaccctcat cttgctgaga aattagaaaa 240 ggtcggtaat cgtgatgtcg ttggtttcgg atggaacggt gagccagtct attacgacag 300 accagatttc ccaatgccag ctgttcgttt caaggaaaac actccagacg ttcttgctct 360 ccgcacaaag gaaaaagaag actggaagaa gctttcagtt caagaaaaga aagccctcta 420 ccgtgcaaca ttctgtcaga cattcgctga gatgaaatat ccaacaggcg aatggaaaat 480 gcacgtcgga aacacattaa ttgtagctgg attggccatc ttcatctcat tatggatggc 540 tacattcatc tacgacgcag aaccagagac attcgatgaa gcccatcgca aggctcaact 600 taaacgtatg cttactttag aagtcaatcc aatccatgga gtttcctcac aatgggacta 660 cgaaaacaag aagtggaaga agtaaattta ttctgtcagg cattaaaagt gacgatagcg 720 tccgactttt cctaaatctt aaaaaaatag aagttattat tttttgtgaa agttcttgtg 780 gaaaaatcag gaacaaaatg taaaattgaa cgaaaaaaaa ttaaaatcag aacacaaaat 840 cgttgaataa agcaaaaaat ccaatggtaa ataaaa 876 <210> 62 <211> 1411 <212> DNA <213> Chironomus riparius <400> 62 tttttttgtt tttttttttt tttggtttat tttaaagata tcgaatgaat caaaatcttt 60 gtctttattt cttgtaaaaa attgtacaca tctttataaa atgtttatgg acctcccaag 120 tatcctaacc tcttagcttc attaatcatt gctttcaagc cagcaacaac ttcaatagca 180 tttggtctga taagacttgg tggcaaaaca aatccaaaat ttccataaac tccatttcct 240 ctcatttcaa ccgtgtagga caatggaatt ccatgggcac catatgcgtg atctggggta 300 gttccactcg cagcatacag tacagtattg ctacttccaa ctgcatagtt aattccatca 360 acagcacgaa gagcatctct aaatgctaca ccaactgata gtaatgttgc attgtttggt 420 ggcatgtttc ttgtgtgacc ccatggatgc aataaataat gtccataaga gtggaatgat 480 atgaacaaat cagtacttct tccatattgt gtaaaaagac tttcaattgc gagtgtctct 540 gcctcactca tactagtagc tccagcaaaa gtgtcgctgc atggtgtggt actagctcca 600 agacttccag tttcatcgcg tactagccag ttgtatccaa agtttctatt tgcgtcaaca 660 ccaaagcata aagcagatac tggtgcacga gttttacgcc agtttctatt gtttgttcgg 720 gagaattcat agccatcagg attagcgttt gtaatgatgt gccagtcaat gtttgcactt 780 aatgcttgga ttgttgggtc agttgatgtc aatagttcat taatcaaata tgtagcagtt 840 gcagatgaaa tccattcacg agaatgaata tttgcttcaa tgataatagc acgattaccc 900 gatcctctgg aaattgaaaa tgatctaatt ggacggcctt cgtgggatgt accaactgtt 960 ctaatatttg ttattgttgg gaaacgaaca tgcatttcat caagccatgc attaatttca 1020 gcaagtgttc catatgaatc ccaatcaatg aaggcagtcg ctttccttct tctgatggca 1080 gcttcttctt catcgattag tgattgataa ttattaatca ccaacttagt cttcattttc 1140 aagttttgtg aaatttcatc aaaatgggca tacatgtgtg gtggtacaag aatttcagct 1200 tcgccattta aaattgctgg ttcaaaaaag tcataagagt cactatgttc ttctaaatat 1260 ttcatggctt catattgatc ttgattttca attgatacac tatagagacg ataattatca 1320 aatctagcct tttctgctcc agcagaaagg aagaaaaaag ccaaacaaat caacagcttc 1380 attataccac aaatatattc cacgcactcg t 1411 <210> 63 <211> 649 <212> DNA <213> Chironomus riparius <400> 63 attgaaaaga ctggtgaatt cttccgtttg atctatgatg ttaagggacg attcactatt 60 caccgtatta ctgctgatga agctaaatac aagttgtgca aggtgaagaa agtcacaatt 120 ggacccaaga agattccata tttagtaaca cacgacggac gtacaattcg ctatccagat 180 ccaacattaa aggttaacga tacagttcaa ttggatattg ctacatcaaa aatcacagac 240 catattcgtt ttgaatcagg caatttagtc atgatcacag gaggtcgtaa tttgggtcgt 300 gtcggaactg tcgtcaacag agaaaaacat ccaggatcct tcgaaattat ccacgtgaaa 360 gatgccaatg gacacacatt cgccacacgt ttaacaaacg tttttaccat cggaaagggc 420 gctaaggcat tcatctcatt accaaagggt aagggtatca aacttagtgt tgctgaagag 480 cgtgacaaga gattggccca aaagagtgca cattaaggaa cacattcatt aatgttaaat 540 taataagcaa gaaaagagca aattttgcaa aggaaatctg caagaaaaaa tgtgaaatat 600 tcctcttgat tcaaaaataa aaaattgaga aaatttgata aaaatcaac 649 <210> 64 <211> 678 <212> DNA <213> Chironomus riparius <400> 64 aactcaccaa ttatagaaca attaaatttt ttccatgatc acgccttatt aattattatt 60 ttagtaacat tatctgtcgg ctatttaata ggaacattat ttttcaataa cttaaataat 120 cgttatttat tacatggtca aactattgaa gtaatttgaa caattttacc cgcaattatt 180 ttattattta ttgctttccc atctttacgg attttatatt tattagacga agtaaataat 240 ccctctattt cattaaaaac tattggtcat caatgatact gaagttacga atattctgat 300 ttttcaggaa ttgaatttga ttcatatata atcccacaaa atgaaatatc attagatact 360 tttcgacttt tagacgtaga taaccgtgtt atcctaccaa ttaataatca gattcgaatt 420 ctagtatcag caactgatgt tcttcattca tgagcaattc catcattagg tgtaaagatt 480 gatgcctctc caggtcgatt aaatcaaacc aattttttta ttaaccgccc aggattattt 540 tttggacaat gttctgaaat ttgtggtgct aatcatagtt ttataccaat tgtaattgaa 600 agtgtcccaa caaatcattt tattaaatga atttctaata taatttaaaa aaaattattt 660 taaaaaaaaa aaaaaact 678 <210> 65 <211> 523 <212> DNA <213> Chironomus riparius <400> 65 tcgaacgtgc tttccaaaag caacaaggag taaatcttaa tcgtaacatc cggttccaag 60 gaaagaaacc aaaatcaact cgtcttcacc gtaatgttgg acttggattc aaaacaccaa 120 aagaggcagt ctctggacat tatatcgata agaaatgtcc attcactggt atggtttcaa 180 ttcgtggaag aattttgact ggagtcgtac gaaaaatgaa gatgcaaaga acaattgtca 240 ttcgtcgtga tttcttacat ttcgttaaaa aatacagtcg ttttgaaaag agacacaaaa 300 atatgagcgt acatttatca ccttgcttcc gcgatgttga gctcggagat gttgtcacag 360 ttggagaatg tcgtccactc tcaaagacag tcaagtttaa tgtattgaag gtcagcaaag 420 ttgctggatc aaagaagaag ttccagaaat tctaaattgt ttaaatacat tcttcattat 480 gtatcaagat ccaagaacat aatattaaat aaaatcaaga aaa 523 <210> 66 <211> 503 <212> DNA <213> Chironomus riparius <400> 66 acgagtgcgt ggaattagaa cagctcgtaa gctcaagaca cacagacgtg atcaacgatg 60 ggccgataag gattacaaaa aggctcattt gggaactcgc tggaaggcaa atccattcgc 120 tggtgcatct catgccaaag gaatcgttct tgaaaaagtc ggtgttgaag ccaaacagcc 180 aaactctgct attcgcaaat gcgtccgtgt acagttaatc aagaatggaa agaaaatcac 240 tgcattcgta ccccgtgatg gttgcttaaa ctacattgaa gaaaacgatg aagttttggt 300 tgctggtttc ggtcgtaaag gtcatgccgt tggtgacatt cccggtgtta gatttaaggt 360 tgttaaggtt gcaaacgttt cactccttgc actttacaaa gaaaagaagg aacgaccaag 420 atcataagct gtaatatgct ggatatttca atgattatat tgaatttcta ataaagctac 480 ataaaatcca atgcttttgg ttt 503 <210> 67 <211> 500 <212> DNA <213> Chironomus riparius <400> 67 ggaactcctc ttggtgctgg gggaattcca gctaattcga actttccaag caagttattg 60 tccttagtca tagctctttc accttcaaag acttgaatca aaactcctgg ttgattgtca 120 gaatatgttg tgaaagtttg agtttgcttg gttggaattg ttgtatttct cttgataaga 180 acgctcataa cacctccagc agtttcaata cctaaagata atggtgttac atccaataaa 240 agcaagtctt gtacttcttc ggacttgtca ccatgcaaaa ttgcggcttg gacagcggca 300 ccataagcga cagcttcatc aggattaata gatttattta attcctttcc gttgaaaaaa 360 tcttgcaaaa gtttttggac ctttggaata cgtgttgatc caccaacaag aacaatatca 420 tgaattgctg ctttgtccat ttttgcatca cgaattgcct tttcgactgg ttccattgtc 480 gaacggaata gatcagcatt 500 <210> 68 <211> 673 <212> DNA <213> Chironomus riparius <400> 68 taaattattt ttagatgttt attatttcag cataataaaa agatgaagaa caaaacaatt 60 agcactttat tcttcctctt gcacgactgt tgtcttctct gagacataca aaccgtcaag 120 gaatttacgg atatccttat ctttaactgt tgtggattgt tgaataaggg cagcagaaag 180 agaaactgct tcaatgtcgt ttccttgcaa aatcaattcg tccttttgct ttgtggagtt 240 gacgactgtc acacctggtt tcattgtaac tttacggata tgcttttctc ccaagaagtt 300 acggatttca atgactgtgt tggcttcaga gatgacacaa ttgatgggga aatgcgcatg 360 tactgctctc atcttgtatt ggaatccctt tgtgacaccc ttgatcatgt tttcgatatg 420 actgcagacg gtacgaacag cagcaatttc tttttggaac cgaaccattt ttcaaccatg 480 acctttttgc gattaaccat tctaatatcc atagcaagat gacggaaatc tcgggtaagt 540 ttacctcttg gtccacggat cgttacaata cgtgccttga cattagcgct gactcctttt 600 gggagtttta caacttgatt gctgttgata gttctcatga tctcaacaaa ttctcgcttt 660 gagtgtcgag cgt 673 <210> 69 <211> 1086 <212> DNA <213> Chironomus riparius <400> 69 aaccgattgg agtttgtggt caaattattc catggaacta tcctgctctt atgacagtgt 60 ttaaaatagc accaattttg gctacaggat gtgttacaat tttaaaacca gcagaacaga 120 ctccattaac tgcactttat attgcaagtt taacaaaaga ggcaggaatt ccagatggtg 180 taattaatgt catcacagga tttggaccaa cagcaggagc agctatttca atgcatcgag 240 atattagaaa ggttgcattc acaggttcaa ctgaaatcgg aaaattgatt atggaagcag 300 ctgcaaaatc aaatttgaag aaagtgtcac ttgaacttgg tggcaaaagt cctttagtca 360 tttttgatga tgtaaatcta gacgaggtag ttccacttgc tcaagaagct atatttacaa 420 attcgggaca aatctgttgc ggtggaagta gaacttttgt tcaagaaaac atttatgatg 480 agtttgttaa acgtacggtt gaacttgcta agaagaggaa agtaggatgt ccatttgatt 540 caaatacaca acaaggtcca caagttgata aaatcacatt tgataagatt cttacttata 600 ttgattatgg aaagcaagac ggtgcaaaat tggaatttgg aggtaaaaga tggggtaatg 660 aagggttttt tgtagaacca accgtcttct caaatgtgac tgatgacatg agaattgcga 720 cagatgaaat tttcggacct gtacaatcaa tcattaagtt caagacatta gatgaagtca 780 tagaaagagc caataacaca gaatacggtc tagcagcagg cattttcaca aagaacttaa 840 acaatgccat cacatttgca aattctgttg aagcaggaac tgtttggatc aattgctata 900 atgcaacaag cgttacttca cctttcggtg gatataagct ctctggaatt ggacgcgagc 960 ttggcgaaga aggaattaat ttatatcttg aaactaaatc tgtatcaatc aacctcgcag 1020 caaataacta aatgatatga catcttcaat aaaaatttga aatttctaaa cgcaaaaaaa 1080 aaaaaa 1086 <210> 70 <211> 1788 <212> DNA <213> Chironomus riparius <400> 70 acgctcgaca ttttaaagtc aagtttgata gataagaaac tagtgcttaa ttttaattga 60 ttaaagaatc agtttgttga tagcagtgat ctcgagcaga ttcgttaaca gaaagctctt 120 taaggttcta gaaatttata tttttgcttc caattctccg ttcatacaaa tttgttaatt 180 atccttttta aaatgagtga gccaatcaga gtttgtgtca ccggcgcggc tggacaaatt 240 gcctactcgt tgctctatat gattgcaaat ggagatgttt ttggtagaga gcaaaaagtc 300 attttgacat tgttggatat tgagccaatg atgggagttt tagagggtgt cgttatggaa 360 ctaagtgatt gcgcttttac attgttgaca caagttatcc caacagccga tccagctgta 420 gctttcaaag atgtgtctgt agctttcctg gttggtgcta tgccaagaag acaaggaatg 480 gaaagaaaag atctgctctc agcaaatgtt aagatcttta aagttcaagg tgaagcatta 540 aacacttatg ctaaaaagga cgtcaaagtt ttggttgttg gcaatccagc aaataccaac 600 gctttgatct gttctcatta tgcaccaaca attccaaaag agaatttcac ggccatgaca 660 agattagacc aaaaccgagc aagagcacaa atcgctcaac gtgttggcac agacatttca 720 aacgtccaga atattatcat ttgggggaat cactcagcaa ctcaatttcc agatgtcgcg 780 aacggtacag ctaatggaaa ggcaataaca gatttagttg atacttcata cttgcagtca 840 gaattcattg agactgttca aaaacgtgga gctgctgtaa ttgctgctcg caaaatgagc 900 tcagcaatgt ctgcagcaaa agcagcagct gatcatgtac gtgattggtg gctcggaact 960 accggaacat tcgtttcaat gggtgtctta tctgatggaa gctataatat tccaaaagat 1020 attgttttct ccttcccagt tgaaattcag aataaacaat ggaaaatcgt tcaaggatta 1080 aacattgacg aatttgcacg tggaaaattg gatattacca caaaagagtt gttggaagaa 1140 aaagaagaag caatggctat ttgcaatgca aactgactag atggatcgaa gttataagaa 1200 taatttcgcc aaatgaattt caagtcgtac ttttcgtttg tctatttcct caagttttct 1260 tatgatttta ttttaccgaa tttatgcgca ttttgcaatt ctaccttcat gtcaataaca 1320 attagaacgt aataatgagg aaaaacatcc accaccaaca agcatttcta cacttcattc 1380 caatacatgc ttaaaccact aatccataaa gttttcagaa aataagagtt tcaaattttt 1440 atctttttct tatttcaaaa ttttctacaa aacaaatctt tatttggaca ctaaatagtc 1500 gatgcatttc aaataaattt taagttaaat actggaaatg gcagtagtgg tttgatcttc 1560 acttctctca aagcttattc tttgttcatc atttgatgca tcaaaatgaa taatagatat 1620 tagatccaat tgtggccatt tccgcctata ttttttattt tcctctcttc taagatgtga 1680 actaaaatca caactttaat tgtaaaaggt tcatattttt tgatattatt ttattattta 1740 agttccttta atttttattt ataatatctg ggttcaaggt ctattttt 1788 <210> 71 <211> 537 <212> DNA <213> Chironomus riparius <400> 71 aacacaaacg ccattgccaa ttgctgcaca ttccaaagac tgtttaacag tgtacatgtt 60 accgatgcac tcaaatgtgt agtcaacaca tccgaattta tcgttcaagt acttgtccat 120 tggaccttca acctcttttg gattaataaa gtcagtgact cccatttcag cagcaatttc 180 acgtttggca tcatttaagt caataccgat gacagttttt gctccacgat gtttacatcc 240 aagtacagtg gcaagtccaa ttcctcctaa accccaaacg gcacagactg aatctggctt 300 gactgctgct gtcttaactg aaccaccata gcctgttggg aatccacaac tcaataagca 360 agcaatatcc aatggtgcag ctggattgat tttagtcaaa ttgaattcct tcaagactgt 420 gtattcagag aatgacgaaa cacccaagaa ttgtaaaagt tgctttccct tgcatgtgat 480 tctcgtggtc tcatcagcca tcaattgacg tgactgagat ccgcttgtga attccag 537 <210> 72 <211> 543 <212> DNA <213> Chironomus riparius <400> 72 gtgttgtaga ttaatgcttt tatttcatag gaatcttaag gatgttagaa tgacaacaca 60 cattgcttag gattgaattt ggtttattcg ttttcttctt catcgtcatc gttcgaactg 120 attctgaagt aacgcaattc atagaggtcc ttatcgtttg agacaacacg gatccaatca 180 cgaagactgt gtttcttcaa atacttcttt gtcaagtact tcaaataacg ctttgagaaa 240 tgaacgtctg aattgacgta gattttgaac ttttgtcttt ccatgttgac attgtttccc 300 aagttgccag tctttcctgc aaccttgaag cgttccttta agaacttttc gaaatcagca 360 acatccaaga tgttgtcttc agcaatgttt gtacaatcaa caccatatct gagatgttcc 420 ttctttttct tcaagccttt gcctcgtaag acctgtttct tttgaactgg tttctttgca 480 tctttagtcg tctttgactt ggctgaagct gggtcgattt tcttggttgt tgcaaccatc 540 ttt 543

Claims (6)

Kkaldagu one or more selected from the group consisting of the nucleotide sequence of SEQ ID NO: 1 to 72 (Chironomus riparius ) Biomarker for the evaluation of ecotoxicity of fullerenes containing genes derived from. Gene chip for evaluating the ecotoxicity of fullerenes comprising one or more of the fungus derived gene selected from the group consisting of the nucleotide sequence of SEQ ID NO: 1 to 72. Kit for full evaluation of ecotoxicity of fullerene containing one or more genes derived from the multi-drugs selected from the group consisting of the nucleotide sequence of SEQ ID NO: 1 to 72. Exposing the funnel to a sample suspected of contaminated with fullerene and isolating RNA therefrom;
Synthesizing cDNA using the separated RNA as a template;
Contacting the synthesized cDNA with the gene chip according to claim 2 and hybridizing with the cDNA;
Detecting a degree of hybridization between the gene chip and cDNA; And
Comparing the detected degree of hybridization with a positive control group. 5. The method of claim 4, wherein the cDNA is labeled with a detectable labeling substance. Composition for evaluating the ecotoxicity of fullerenes comprising one or more genera derived gene selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 72.

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