KR20130074796A - DNA Polymorphism Marker for Identification of Cucumis sativus L. - Google Patents
DNA Polymorphism Marker for Identification of Cucumis sativus L. Download PDFInfo
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Abstract
Description
본 발명은 오이의 품종을 식별하기 위한 다형성 마커, 오이 품종 식별 방법 및 상기 마커를 포함하는 키트에 관한 것이다. The present invention relates to a polymorphic marker for identifying a variety of cucumbers, a method for identifying a cucumber variety, and a kit comprising the marker.
식물의 품종간 구분은 여러 가지 목적에 따라 필요한 연구이다. 예를 들면, 국제식물신품종보호동맹 UPOV(International Uion for the Protection of New Varieties of Plant)의 설립이후 품종보호권 설정은 종자회사와 육종가의 큰 관심사가 되었고, UPOV의 회원국인 우리나라 또한 품종보호를 위하여 품종구분에 객관적인 기준을 설정 혹은 수치화하는 계량화의 필요성을 인식하게 되었다. The distinction between plant varieties is a necessary study for various purposes. For example, since the establishment of the International Uion for the Protection of New Varieties of Plant (UPOV), the establishment of variety protection rights has become a great concern for seed companies and breeders. I came to recognize the necessity of quantification to establish or quantify objective criteria for classification.
일반적인 품종 구분은 표현형에 근거하여 구분되어 왔다. 하지만 표현형은 환경요인에 많은 영향을 받기 때문에 통계적으로 많은 실험을 요구한다. 또한 품종간 구별에 있어 표현형은 객관적 기준이 불분명하고 체감적인 차이를 반영하지 못한다. 이런 어려움을 극복하기 위해 DNA 분자 마커를 개발하기 시작했다. General cultivar classification has been classified based on phenotype. However, since phenotype is highly influenced by environmental factors, statistically many experiments are required. In addition, in the distinction between varieties, the objective criterion for phenotype is unclear and does not reflect tangible differences. To overcome these difficulties, they began to develop DNA molecular markers.
DNA 분자 마커는 RFLP(restriction fragment length polymorphism), RAPD(random amplified polymorphic DNA), SSR(simple sequence repeat), SNP(Single-nucleotide polymorphism) 등 여러 가지 종류가 있다. 이중에 SNP 마커는 DNA 염기 변이 형태의 가장 흔한 유형이며 다형성 마커 개발에 높은 성공의 결과를 나타내는 방법 중 하나이다. There are several types of DNA molecular markers, such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and single-nucleotide polymorphism (SNP). Among them, SNP markers are the most common type of DNA base variant form and are one of the methods with high success in developing polymorphic markers.
오이(Cucumis sativus L.) 는 인도 서북부의 히말라야 산맥 남부가 원산지이며, 약 3,000년 전부터 재배되어 오고 있는 작물로서, 이집트에서는 기원전 1,750년경에 재배되었고, 남유럽에는 기원 전후에 전파되었으며, 유럽에서는 16세기경에 재배되었으나 중국에서는 6세기경부터 널리 재배된 것으로 보고된다. 우리나라에서는 약 1,500년 전에 도입된 것으로 알려지고 있는데, 오이는 많은 종류의 품종이 있지만 주로 화남형, 화북형, 피클형 오이가 재배된다.Cucumber ( Cucumis sativus L. ) is a crop that is native to the southern Himalayas of northwest India and has been cultivated for about 3,000 years.It was cultivated in Egypt around 1,750 B.C., and spread around B.C. It was cultivated around the circumference, but in China it is reported to have been cultivated widely from around the 6th century. In Korea, it is known that it was introduced about 1,500 years ago. There are many kinds of cucumbers, but mainly Hwanam-type, Hwabuk-type, and pickle-type cucumbers are grown.
오이의 주요성분은 약 95%가 수분이며, 단백질, 지질, 당질의 함량이 낮아 칼로리는 매우 낮고, 그 밖에 칼륨, 비타민 A와 비타민 C를 함유하고 있다. 특히 오이가 함유하는 비타민 C는 산화효소에 의해 쉽게 파괴될 수 있다. 또한, 오이에 함유된 칼륨은 과잉의 염분을 체외로 배출시키는 작용을 하며, 이뇨작용을 촉진시켜 신장병 환자나 고혈압 환자에게 좋다. 오이는 목이 마르고 아플 때, 특히 더위를 먹었을 때 섭취하면 좋다고 보고된다. 뿐만 아니라 최근 일본에서는 오이가 사람의 신체 내에서 각종 해독작용을 하는 것으로 보고됨에 따라 앞다투어 오이를 이용한 기능성 식품개발에 박차를 가하고 있으며, 국내에서도 이러한 움직임이 보이고 있다.Cucumber's main ingredient is about 95% water, and its low content of protein, lipid, and sugar makes it very low in calories. In addition, it contains potassium, vitamin A and vitamin C. In particular, vitamin C contained in cucumbers can be easily destroyed by oxidative enzymes. In addition, potassium contained in cucumbers acts to discharge excess salt out of the body, and promotes diuresis, which is good for patients with kidney disease or hypertension. Cucumbers are reported to be good for consumption when thirsty and sick, especially in the heat. In addition, as cucumbers are recently reported to have various detoxification effects in the human body in Japan, they are striving to develop functional foods using cucumbers, and such a movement has been shown in Korea.
오이는 7개 (2n=14)의 염색체를 가진다. 국외에서는 오이와 멜론을 대상으로 분자 마커가 개발되었고 이를 이용한 유전학적 연구가 이루어져 왔다 (Silberstein et al.1999; Staub et al.2004; Zhuang et al.2004; Lopez-Sese et al.2002; Monforte et al.2003; Danin-Poleg et al.1998; Danin-Poleg et al.2001 Chiba et al.2003; Fazio et al. 2002). 하지만 이런 연구를 통해 오이의 경우 품종간 유전적 차이가 상당히 적어 다형성 마커의 개발이 어려운 것으로 알려졌다. 특히 같은 지역 또는 비슷한 유전적 기원을 가지는 오이에서 개발되어온 오이 품종들은 서로 상당히 유전적으로 가까워서 다형성 마커의 개발이 어렵다.Cucumber has 7 chromosomes (2n=14). In foreign countries, molecular markers have been developed for cucumbers and melons, and genetic studies have been conducted using them (Silberstein et al.1999; Staub et al.2004; Zhuang et al.2004; Lopez-Sese et al.2002; Monforte et al.2002; Monforte et al. al. 2003; Danin-Poleg et al. 1998; Danin-Poleg et al. 2001 Chiba et al. 2003; Fazio et al. 2002). However, it is known that the development of polymorphic markers is difficult in the case of cucumbers through these studies because the genetic differences between varieties are quite small. In particular, cucumber cultivars that have been developed from cucumbers having the same region or similar genetic origin are very close to each other genetically, making it difficult to develop polymorphic markers.
이에 본 발명자들은 유전적 차이가 적은 오이 품종 간에 구분이 가능할 수 있는 다량의 SNP 기반 다형성 분자 마커를 개발하고자 연구를 계속한 결과, 오이 염기서열 중 다형성 지역을 확보하고 프라이머를 제작함으로써, 본 발명을 완성하였다. Accordingly, the present inventors continued research to develop a large amount of SNP-based polymorphic molecular marker that can distinguish between cucumber varieties with little genetic difference. As a result, by securing a polymorphic region among cucumber nucleotide sequences and preparing a primer, the present invention Completed.
본 발명의 목적은, 오이 품종 식별용 마커를 제공하는 것이다. An object of the present invention is to provide a marker for identifying cucumber varieties.
본 발명의 또 다른 목적은, 오이 품종을 식별하는 방법 및 오이 품종 식별용 키트를 제공하는 것이다. Another object of the present invention is to provide a method for identifying cucumber varieties and a kit for identifying cucumber varieties.
상기 과제를 해결하기 위하여, 본 발명은 오이의 16개 품종에 대해 높은 다형성을 나타내는 오이 품종 식별용 마커를 제공한다. In order to solve the above problems, the present invention provides a marker for identifying a cucumber variety that exhibits high polymorphism for 16 varieties of cucumber.
또한, 본 발명은 오이로부터 유래된 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고 상기 마커를 프라이머로 이용하여 게놈 DNA를 증폭하는 단계; 및 상기 증폭된 산물을 분석하여 오이의 품종을 식별하는 단계를 포함하는 오이 품종을 식별하는 방법을 제공한다. In addition, the present invention is to separate the genomic DNA derived from cucumber; Amplifying genomic DNA using the isolated genomic DNA as a template and using the marker as a primer; And it provides a method for identifying a cucumber variety comprising the step of identifying the variety of cucumber by analyzing the amplified product.
또한, 본 발명은 상기 마커를 포함하는 오이 품종 식별용 키트를 제공한다. In addition, the present invention provides a kit for identifying cucumber varieties comprising the marker.
본 발명의 오이 품종 식별용 마커를 이용하여 국내에서 보급되고 있는 오이 16개 품종을 식별할 수 있어, 국내 오이 품종 구분 및 오이 품종 육성에 유용하게 사용될 수 있다. It is possible to identify 16 varieties of cucumbers that are spreading in Korea using the marker for identifying cucumber varieties of the present invention, and thus can be usefully used for distinguishing domestic cucumber varieties and cultivating cucumber varieties.
도 1은 16종의 오이 품종을 나타낸 도표이다.
도 2 내지 도3은 오이의 다형성 지역을 바탕으로 총 60개의 마커를 나타낸 도표이다.
도 4는 총 60개의 프라이머를 이용하여 다형성 수준을 분석한 도표이다.
도 5는 M42 마커를 이용하여 국내외 품종을 구분한 그림이다. (G : GY-14, N : NC76)
도 6은 다양한 마커를 이용하여 HRM을 통해 오이 품종을 구분한 그림이다.
(M12의 그룹 1 : 국내 오이품종, 그룹 2: 중국품동 (Donggwan), 그룹 3: 미국품종 (GY-14, NC76), M6의 그룹 1: 백다다기 품종, M47의 그룹 1: 동부한농 품종) 1 is a chart showing 16 kinds of cucumber varieties.
2 to 3 are diagrams showing a total of 60 markers based on the polymorphic region of cucumber.
Figure 4 is a chart analyzing the level of polymorphism using a total of 60 primers.
5 is a diagram showing domestic and foreign varieties using the M42 marker. (G: GY-14, N: NC76)
6 is a diagram showing cucumber varieties classified through HRM using various markers.
(M12's Group 1: Domestic Cucumber Varieties, Group 2: Chinese Pumdong (Donggwan), Group 3: American Varieties (GY-14, NC76), M6's Group 1: Baekdagi Varieties, M47's Group 1: Dongbu Hannong Varieties)
이하 본 발명에 대하여 보다 상세히 설명한다. Hereinafter, the present invention will be described in more detail.
본 발명은 오이의 16개 품종에 대해 높은 다형성을 나타내는 오이 품종 식별용 마커을 제공한다.The present invention provides a marker for identifying a cucumber variety showing high polymorphism for 16 varieties of cucumber.
바람직하게는 본 발명의 마커는 하기 도 2 내지 도 3에 기재된 염기서열 (서열번호 1 내지 120)로 이루어진 군으로부터 선택되는 하나 이상의 마커를 포함한다. Preferably, the marker of the present invention includes one or more markers selected from the group consisting of the nucleotide sequences (SEQ ID NOs: 1 to 120) described in FIGS. 2 to 3 below.
본 발명에서 사용하는 용어인 “마커”란 오이를 품종별로 구분할 수 있는 물질로, 오이 품종에 따라 특이적으로 존재하는 폴리펩타이드 또는 핵산 (예: mRNA 등), 지질, 당지질, 당단백질, 당(단당류, 이당류, 올리고당류 등) 등과 같은 유기 생체 분자 등을 포함한다. The term “marker” used in the present invention is a substance that can classify cucumbers by cultivar. Polypeptides or nucleic acids (eg, mRNA, etc.), lipids, glycolipids, glycoproteins, sugars ( Organic biomolecules such as monosaccharides, disaccharides, oligosaccharides, etc.) are included.
본 발명의 “프라이머 (primer)”란 적절한 버퍼 중의 적절한 조건 (예를 들면, 4개의 다른 뉴클레오시드 트리포스페이트 및 DNA, RNA 폴리머라제 또는 역전사 효소와 같은 중합제) 및 적당한 온도 하에서 주형-지시 DNA 합성의 시작점으로서 작용할 수 있는 단일가닥 올리고뉴클레오티드를 말한다. 상기 프라이머의 적절한 길이는 사용 목적에 따라 달라질 수 있으나, 통상 15 내지 30 뉴클레오티드이다. 짧은 프라이머 분자는 일반적으로 주형과 안정한 혼성체를 형성하기 위해서는 더 낮은 온도를 필요로 한다. 프라이머 서열은 주형과 완전하게 상보적일 필요는 없으나, 주형과 혼성화할 정도로 충분히 상보적이어야 한다. 본 발명의 프라이머는 예를 들면, 포스포르아미다이트 고체 지지체 방법과 같은 당 분야에 공지된 방법을 이용하여 화학적으로 합성할 수 있다. 또한, 공지된 방법으로 메틸화, 캡화 등으로 변형시킬 수 있다.The term "primer" of the present invention refers to a template-directed DNA under appropriate conditions in an appropriate buffer (eg, 4 different nucleoside triphosphates and a polymerizing agent such as DNA, RNA polymerase or reverse transcriptase) and an appropriate temperature. It refers to a single-stranded oligonucleotide that can serve as a starting point for synthesis. The appropriate length of the primer may vary depending on the intended use, but is usually 15 to 30 nucleotides. Short primer molecules generally require a lower temperature to form a stable hybrid with the template. The primer sequence need not be completely complementary to the template, but must be sufficiently complementary to hybridize to the template. The primer of the present invention can be chemically synthesized using a method known in the art, such as, for example, a phosphoramidite solid support method. In addition, it can be modified by methylation or capping by a known method.
본 발명에 있어서 "특이적"이라는 용어는 다른 유전자에 결합하지 않고 상기 유전자의 특정부위에만 특이적으로 결합하는 것을 의미한다.In the present invention, the term "specific" refers to specifically binding only to a specific region of the gene without binding to other genes.
또한, 본 발명은 오이로부터 유래된 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고 상기 마커를 프라이머로 이용하여 게놈 DNA를 증폭하는 단계; 및 상기 증폭된 산물을 분석하여 오이의 품종을 식별하는 단계를 포함하는 오이 품종을 식별하는 방법을 제공한다. In addition, the present invention comprises the steps of separating genomic DNA derived from cucumber; Amplifying genomic DNA using the isolated genomic DNA as a template and using the marker as a primer; And it provides a method for identifying a cucumber variety comprising the step of identifying the variety of cucumber by analyzing the amplified product.
본 발명의 오이 품종 식별 방법에서 게놈 DNA의 증폭은 PCR(polymerase chain reaction) 을 사용하는 것이 바람직하다. 일반적인 PCR 수행 방법에 대해서는 당업계에 잘 알려져 있다. 구체적으로, PCR 반응은 당업계에서 PCR반응에 필요하다고 알려진 여러 성분을 포함하는 PCR 반응액을 이용하여 수행될 수 있다. 예를 들어, 상기 PCR 반응액은 분석하고자 하는 오이로부터 유래된 게놈 DNA, 본 발명의 마커, 적당량의 DNA 중합 효소(예를 들면, Taq polymerase), dNTP 혼합물, PCR 완충용액(buffer) 및 물을 포함한다. 상기 PCR 완충용액은 KCl, Tris-HCl 및 MgCl2를 함유할 수 있다.In the method for identifying cucumber varieties of the present invention, it is preferable to use PCR (polymerase chain reaction) for amplification of genomic DNA. A general method of performing PCR is well known in the art. Specifically, the PCR reaction may be performed using a PCR reaction solution containing various components known to be necessary for a PCR reaction in the art. For example, the PCR reaction solution contains genomic DNA derived from a cucumber to be analyzed, a marker of the present invention, an appropriate amount of DNA polymerase (e.g., Taq polymerase), a dNTP mixture, a PCR buffer, and water. Includes. The PCR buffer solution may contain KCl, Tris-HCl and MgCl2.
본 발명에서 상기 증폭된 산물은 전기영동에 의해 확인하는 것이 바람직하나 이에 제한되는 것은 아니다. 본 발명에서 전기영동 시 사용하는 겔은 통상적으로 전기영동에서 사용할 수 있는 겔을 사용할 수 있고, 대표적인 예로 아가로스 겔 또는 폴리아크릴아미드 겔을 사용할 수 있다. 전기영동 후 실버 염색(silver staining)으로 전기영동 결과를 분석할 수 있다. 일반적인 전기영동 분석 방법에 대해서는 당업계에 잘 알려져 있다. 본 발명의 오이 품종 식별 방법에서 증폭된 산물을 전기영동으로 확인한 후, 조합된 밴드들의 분석을 통하여 각각의 품종을 식별할 수 있다. In the present invention, the amplified product is preferably confirmed by electrophoresis, but is not limited thereto. The gel used for electrophoresis in the present invention may be a gel that can be used in general electrophoresis, and as a representative example, an agarose gel or a polyacrylamide gel may be used. After electrophoresis, the electrophoresis results can be analyzed by silver staining. General electrophoretic analysis methods are well known in the art. After confirming the amplified product by electrophoresis in the cucumber variety identification method of the present invention, each variety can be identified through analysis of the combined bands.
또한 본 발명의 오이 품종 식별 방법은 HRM (high resolution melting) 시스템을 통해 이루어질 수 있으며 일반적인 HRM 수행 방법에 대해서는 당업계에 잘 알려져 있다.In addition, the method of identifying cucumber varieties of the present invention may be performed through a high resolution melting (HRM) system, and a general method of performing HRM is well known in the art.
또한, 본 발명은 상기 마커를 포함하는 오이 품종 식별용 키트를 제공한다. In addition, the present invention provides a kit for identifying cucumber varieties comprising the marker.
본 발명의 키트는 본 발명의 프라이머 세트 중 하나 이상, DNA 중합 효소 및 PCR 완충용액(buffer)을 포함하는 오이 품종 식별용 키트를 제공한다. PCR 완충용액에 포함되는 성분들은 상기에서 기술한 바와 같다. 이외에 PCR산물의 증폭 여부를 확인할 수 있는 전기영동 수행에 필요한 구성 성분들이 본 발명의 키트에 추가로 포함될 수 있다.
The kit of the present invention provides a kit for identifying cucumber varieties comprising at least one of the primer sets of the present invention, a DNA polymerase, and a PCR buffer. Components included in the PCR buffer solution are as described above. In addition, constituents necessary for electrophoresis that can determine whether a PCR product is amplified may be additionally included in the kit of the present invention.
이하, 본 발명을 실시예에 의거하여 보다 구체적으로 설명한다. 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.
Hereinafter, the present invention will be described in more detail based on examples. The examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the invention is not limited by these examples according to the gist of the present invention.
실시예 1. 오이 품종 및 DNA 추출Example 1. Cucumber varieties and DNA extraction
국내에서 시판하는 오이품종과 국외에서 시판하는 오이품종으로 총 16종의 오이를 사용하여 실험하였다 (도 1). DNA는 동결 건조된 어린 잎 조직으로부터 CTAB 방법으로 추출하였다.
A total of 16 kinds of cucumbers were used as a cucumber variety sold in Korea and a cucumber variety sold overseas (FIG. 1). DNA was extracted from freeze-dried young leaf tissue by the CTAB method.
실시예 2. 오이 염기서열 다형성 지역 확보 및 프라이머 제작Example 2. Securing cucumber nucleotide sequence polymorphism region and preparation of primer
오이 염기서열은 GY-14 품종과 Long4 품종을 이용하여 두 품종사이에서 염기서열 차이가 심한 정도를 SNP 수와 Indel (insertion/deletion)의 수 정도로 구분하였다 (도 4). 이러한 방법으로 60개 지역이 선정되었고 이 염기서열 다형성 지역을 PCR 증폭이 가능한 프라이머를 제작하였다 (도 2 및 도 3). 프라이머의 Tm 온도는 섭씨 54도 기준으로 제작하였다. 프라이머는 Genotech Corp. (Daejeon, Korea)에서 구입하였다.
Cucumber nucleotide sequence was classified as the number of SNPs and the number of Indels (insertion/deletion) to the extent that the nucleotide sequence difference between the two cultivars was severe using GY-14 cultivar and Long4 cultivar (FIG. 4). In this way, 60 regions were selected, and primers capable of PCR amplification of this nucleotide sequence polymorphic region were prepared (FIGS. 2 and 3). The Tm temperature of the primer was prepared based on 54 degrees Celsius. The primer is Genotech Corp. (Daejeon, Korea).
실시예 3. PCR 증폭, 전기영동, HRM (High resolution melting) 분석Example 3. PCR amplification, electrophoresis, HRM (high resolution melting) analysis
PCR의 총 볼륨은 15ul로 10mM dNTP 0.3ul, 10x buffer는 1.5ul, Taq polymerase는 0.4 unit, 100uM의 Foward 프라이머, Reverse프라이머를 각각 1ul, DNA는 15ng을 사용하였다. PCR은 섭씨 95도에서 3분 후 95도에서 5초, 50도에서 10초, 68도에서 20초 조건을 50번 반복 하였다. PCR이 끝난 후 전기영동으로 증폭 유무와 증폭된 DNA 조각 크기를 확인하였다. EtBr이 첨가된 2% 아가로즈 겔 (20×40Cm)에서 0.5× TBE 버퍼에 담군 후 300V에서 약 2시간동안 전기 영동하였고, DNA 증폭 밴드의 위치를 UV trans-illuminator로 확인하였다. 염기서열 차이를 구분하기 위하여 HRM 분석은 Light scanner (Idaho Co. USA)를 사용하였다. The total volume of PCR was 15ul, 0.3ul of 10mM dNTP, 1.5ul of 10x buffer, 0.4 unit of Taq polymerase, 1ul of 100uM of forward primer, reverse primer, and 15ng of DNA. PCR was repeated 50 times at 95°C for 3 minutes, followed by 5 seconds at 95°C, 10 seconds at 50°C, and 20 seconds at 68°C. After the PCR was completed, the presence or absence of amplification and the size of the amplified DNA fragment were checked by electrophoresis. After immersing in 0.5× TBE buffer on a 2% agarose gel (20×40Cm) to which EtBr was added, electrophoresis was performed at 300V for about 2 hours, and the position of the DNA amplification band was confirmed with a UV trans-illuminator. HRM analysis was performed using a light scanner (Idaho Co. USA) to distinguish nucleotide sequence differences.
전기영동 결과 모든 프라이머 조합에서 증폭된 DNA 밴드를 관찰 할 수 있었고 그 중 전기영동에서 크기 구분이 가능한 것은 M42 프라이머 조합으로 나타났다 (도 5). M42에서 DNA 크기가 다른 2개의 오이 품종은 NC-76과 GY-14 으로 미국 품종이었다. 이 결과는 오이 품종이 품종 간에 유전적 차이가 적다는 것을 다시 한 번 확인할 수 있었고 특히 국내 오이 품종 간에는 차이를 발견할 수 없었음을 확인하였다. As a result of electrophoresis, DNA bands amplified in all primer combinations could be observed, and among them, the M42 primer combination was shown to be size-distinguishable in electrophoresis (Fig. 5). Two cucumber cultivars with different DNA sizes in M42 were the American cultivar NC-76 and GY-14. This result confirmed once again that the genetic difference between the cucumber varieties was small, and in particular, no differences were found between the domestic cucumber varieties.
또한 DNA 염기서열 차이를 염기분석 없이 다형성을 검사하기 위해 HRM을 수행하였다. HRM은 염기서열 차이에 의해 DNA조각의 melting 온도 차이에 의한 melting curve 차이를 기준으로 구분해 주는 기술이다. In addition, HRM was performed to test for polymorphism without base analysis for DNA sequence differences. HRM is a technology that classifies the difference in the melting curve due to the difference in melting temperature of DNA fragments based on the difference in base sequence.
HRM 분석에 의한 오이 품종간의 차이를 보여주는 결과를 도 6에 나타내었다. M12 마커에서 미국 품종인 NC-76, GY-14 과 중국품종인 Donggwan, 그리고 국내품종의 구분이 가능하였다. The results showing the difference between cucumber varieties by HRM analysis are shown in FIG. 6. In the M12 marker, it was possible to distinguish between NC-76, GY-14, Chinese varieties, Donggwan, and domestic varieties.
또한, M6 마커는 조은 백다다기를 제외한 은성, 흑농, 백록, 한강 백다다기 품종이 다른 것과 구별되어 그룹화 되었다. M20, M47의 경우 동부 한농에서 구입한 품종인 신정품, White, 낙동청장은 다른 오이 품종들로부터 분리되어 그룹화 되었다. 이를 토대로 16가지 오이는 기본적으로 국가별로 유전적 차이를 보이며, 국내품종 사이에서는 각각 은성, 흑농, 백록, 한강백다다기와 신정품, White, 낙동청장이 유전적으로 가깝다고 추측 할 수 있다.In addition, the M6 markers were grouped by distinguishing from other varieties of Eunseong, Heuknong, Baekrok, and Hangang Baekdagi except for the Joeun Baekdagi. In the case of M20 and M47, the varieties purchased from Dongbu Hannong, New Genpum, White, and Nakdong Cheongjang were separated from other cucumber varieties and grouped. Based on this, it can be assumed that 16 kinds of cucumbers basically show genetic differences by country, and between domestic varieties, Eunseong, Heuknong, Baekrok, Hangang Baekdadagi, New Genpum, White, and Nakdong Cheongjang are genetically close, respectively.
<110> Dongguk University Industry-Academic Cooperation Foundation <120> DNA Polymorphism Marker for Identification of Cucumis sativus L. <160> 120 <170> KopatentIn 1.71 <210> 1 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M1 F Primer of Cucumis sativus L. <400> 1 catttgaaag ttcaaccctt ctaaaca 27 <210> 2 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M1 R Primer of Cucumis sativus L. <400> 2 cctaaacttt gaaaagtaac aatttactc 29 <210> 3 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M2 F Primer of Cucumis sativus L. <400> 3 aatcctgaag ttcatgttga tgtgg 25 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M2 R Primer of Cucumis sativus L. <400> 4 ctctctaggc cataaactat cctc 24 <210> 5 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M3 F Primer of Cucumis sativus L. <400> 5 tgttccactc tacctatgac attttga 27 <210> 6 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M3 R Primer of Cucumis sativus L. <400> 6 accaaatgta tatgtttttc tttgcattt 29 <210> 7 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M4 F Primer of Cucumis sativus L. <400> 7 ttgtttacac cgtgtcagta acttaag 27 <210> 8 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> M4 R Primer of Cucumis sativus L. <400> 8 cgtgtgtaaa aattaaattc aaaacatttc 30 <210> 9 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M5 F Primer of Cucumis sativus L. <400> 9 ttttcgagca atcaaatggt ttttattta 29 <210> 10 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M5 R Primer of Cucumis sativus L. <400> 10 tgctactttc ttgagatgaa actttttg 28 <210> 11 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M6 F Primer of Cucumis sativus L. <400> 11 tgattgtgac tttgcaagga aatg 24 <210> 12 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M6 R Primer of Cucumis sativus L. <400> 12 tcttaagaga tggcccaacc aa 22 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M7 F Primer of Cucumis sativus L. <400> 13 ttgctgaatg caccaatgga 20 <210> 14 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M7 R Primer of Cucumis sativus L. <400> 14 ccatggaagg tattgagaaa ttgatg 26 <210> 15 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M8 F Primer of Cucumis sativus L. <400> 15 tcttaattaa ccgagagaca cttcc 25 <210> 16 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M8 R Primer of Cucumis sativus L. <400> 16 tcgttaaatt tgtatacgaa aaatttgat 29 <210> 17 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> M9 F Primer of Cucumis sativus L. <400> 17 aatctttact tgtatcaatc ttctaaattc 30 <210> 18 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M9 R Primer of Cucumis sativus L. <400> 18 caatttaaac ctcaaaactt ctttaaag 28 <210> 19 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M10 F Primer of Cucumis sativus L. <400> 19 tgaataaata ttttaaggtc gtatacag 28 <210> 20 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M10 R Primer of Cucumis sativus L. <400> 20 tccaccaaac cacactactc tt 22 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M11 F Primer of Cucumis sativus L. <400> 21 cgtctgtgct atacgccaag 20 <210> 22 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M11 R Primer of Cucumis sativus L. <400> 22 ttcaagcact taaagataag ccaa 24 <210> 23 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M12 F Primer of Cucumis sativus L. <400> 23 tatagaagat tagaagaaat acgttg 26 <210> 24 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M12 R Primer of Cucumis sativus L. <400> 24 aaccttacta gggtttacat tcgttt 26 <210> 25 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M13 F Primer of Cucumis sativus L. <400> 25 aaacgtgaag taatgagacc tagcgta 27 <210> 26 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M13 R Primer of Cucumis sativus L. <400> 26 catttgtaaa catcaattcg ccaaa 25 <210> 27 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M14 F Primer of Cucumis sativus L. <400> 27 tctctgtgtt ggtataacgt acca 24 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M14 R Primer of Cucumis sativus L. <400> 28 caagctaagt gagaatgtaa gtg 23 <210> 29 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M15 F Primer of Cucumis sativus L. <400> 29 tacagaattt gtgtttaaat tatttgtt 28 <210> 30 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M15 R Primer of Cucumis sativus L. <400> 30 atgtgatcaa atacatttat aaagg 25 <210> 31 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M16 F Primer of Cucumis sativus L. <400> 31 cttatatatt gttgatcaag ctattg 26 <210> 32 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M16 R Primer of Cucumis sativus L. <400> 32 gcacttacga ggataaacaa aatac 25 <210> 33 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M17 F Primer of Cucumis sativus L. <400> 33 aggtgcattt gggtagaatg ttg 23 <210> 34 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M17 R Primer of Cucumis sativus L. <400> 34 cagtgattta tttttgcacg gaact 25 <210> 35 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M18 F Primer of Cucumis sativus L. <400> 35 tccaaaaaga tcaattaact caagtctca 29 <210> 36 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M18 R Primer of Cucumis sativus L. <400> 36 ccggtccatc aactactact caagg 25 <210> 37 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> M19 F Primer of Cucumis sativus L. <400> 37 gccccaactc gctcgatg 18 <210> 38 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M19 R Primer of Cucumis sativus L. <400> 38 tgaaataaga tcacaaaaag gggc 24 <210> 39 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M20 F Primer of Cucumis sativus L. <400> 39 gcatacattg aaatgcaaca actca 25 <210> 40 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M20 R Primer of Cucumis sativus L. <400> 40 tcccctttag atgtccaata aatgc 25 <210> 41 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M21 F Primer of Cucumis sativus L. <400> 41 ttgatgaaga agcgtttaca gtatgg 26 <210> 42 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M21 R Primer of Cucumis sativus L. <400> 42 agtggatttg aaacgcaagg at 22 <210> 43 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M22 F Primer of Cucumis sativus L. <400> 43 tggccttaca attgctattt tcca 24 <210> 44 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M22 R Primer of Cucumis sativus L. <400> 44 gccttttcct attcgacttt tgtct 25 <210> 45 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M23 F Primer of Cucumis sativus L. <400> 45 aaagttaact catcgcaaaa ttgttg 26 <210> 46 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M23 R Primer of Cucumis sativus L. <400> 46 ggccaaaagt attagtccgt ag 22 <210> 47 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M24 F Primer of Cucumis sativus L. <400> 47 tcaaagtatt cattaagaaa attgcat 27 <210> 48 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M24 R Primer of Cucumis sativus L. <400> 48 tgaaaggggg ttagttttta aaga 24 <210> 49 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M25 F Primer of Cucumis sativus L. <400> 49 gttggttaaa tatatttttt tacaacttg 29 <210> 50 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M25 R Primer of Cucumis sativus L. <400> 50 aaacagacaa cttttctaat atacg 25 <210> 51 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M26 F Primer of Cucumis sativus L. <400> 51 tggcttcttg ccagattcca tt 22 <210> 52 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M26 R Primer of Cucumis sativus L. <400> 52 tctcttgaaa gttcctttga gatct 25 <210> 53 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M27 F Primer of Cucumis sativus L. <400> 53 aagaactgac tataaagatt aatttta 27 <210> 54 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M27 R Primer of Cucumis sativus L. <400> 54 taactcttag acatgtgagt atac 24 <210> 55 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M28 F Primer of Cucumis sativus L. <400> 55 ctaatatgga atgaaaaaca aggttg 26 <210> 56 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M28 R Primer of Cucumis sativus L. <400> 56 ttaactctta gacatgtgag tatact 26 <210> 57 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M29 F Primer of Cucumis sativus L. <400> 57 caacatcaac atgaccccat aa 22 <210> 58 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M29 R Primer of Cucumis sativus L. <400> 58 ttgcactgga agttctgaaa agg 23 <210> 59 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M30 F Primer of Cucumis sativus L. <400> 59 ctgagaaaaa tggcggcaag 20 <210> 60 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M30 R Primer of Cucumis sativus L. <400> 60 gggacattgt cgatgagatt ga 22 <210> 61 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M31 F Primer of Cucumis sativus L. <400> 61 gcgattttaa tcaaaaattt cagtg 25 <210> 62 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M31 R Primer of Cucumis sativus L. <400> 62 tcttccaata tactctctcc ct 22 <210> 63 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M32 F Primer of Cucumis sativus L. <400> 63 tgcaatgtgt cattccccat tta 23 <210> 64 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M32 R Primer of Cucumis sativus L. <400> 64 ccctatacct ctacattcat ctc 23 <210> 65 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M33 F Primer of Cucumis sativus L. <400> 65 tgtaatttgc cattttaaga accttga 27 <210> 66 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M33 R Primer of Cucumis sativus L. <400> 66 gaaggaaaat tggttggaat ttcg 24 <210> 67 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M34 F Primer of Cucumis sativus L. <400> 67 catgttttta ataattaaaa ataaatcat 29 <210> 68 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M34 R Primer of Cucumis sativus L. <400> 68 atgataaata ataactttta gttcg 25 <210> 69 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M35 F Primer of Cucumis sativus L. <400> 69 tgatcgtagt tattctccct tcaa 24 <210> 70 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> M35 R Primer of Cucumis sativus L. <400> 70 gaaaaaggac taaaaatgtg aataaataag 30 <210> 71 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M36 F Primer of Cucumis sativus L. <400> 71 catcgctttc aaaagaggtt gg 22 <210> 72 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M36 R Primer of Cucumis sativus L. <400> 72 gaacctccaa atgggattca gg 22 <210> 73 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M37 F Primer of Cucumis sativus L. <400> 73 tgcaatcaca gaaatggaac cag 23 <210> 74 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M37 R Primer of Cucumis sativus L. <400> 74 tcaacttttg atgcaaactg atgg 24 <210> 75 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M38 F Primer of Cucumis sativus L. <400> 75 cgcgaagaag aagaggcaga 20 <210> 76 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M38 R Primer of Cucumis sativus L. <400> 76 ccctagcttt gtgcgacgtt 20 <210> 77 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M39 F Primer of Cucumis sativus L. <400> 77 cagtcgctgg gccttctttc 20 <210> 78 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M39 R Primer of Cucumis sativus L. <400> 78 ttcaggagac aaagcgagac ctt 23 <210> 79 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M40 F Primer of Cucumis sativus L. <400> 79 tattggatat ccacattgtg acag 24 <210> 80 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M40 R Primer of Cucumis sativus L. <400> 80 ggatttctgt tttgatggat agaac 25 <210> 81 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M41 F Primer of Cucumis sativus L. <400> 81 gtaattaaat accacataat atcacaa 27 <210> 82 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M41 R Primer of Cucumis sativus L. <400> 82 tgttcaagag gctatacatc cta 23 <210> 83 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M42 F Primer of Cucumis sativus L. <400> 83 ggattccaaa gaatttgttc tcca 24 <210> 84 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M42 R Primer of Cucumis sativus L. <400> 84 gccttatggg tttaaattca atactc 26 <210> 85 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M43 F Primer of Cucumis sativus L. <400> 85 atagatatgt acgtcgattt ctcca 25 <210> 86 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M43 R Primer of Cucumis sativus L. <400> 86 taggctttat attttggatt aagttg 26 <210> 87 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M44 F Primer of Cucumis sativus L. <400> 87 ctttcaagaa ctattaacca ttgc 24 <210> 88 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M44 R Primer of Cucumis sativus L. <400> 88 cgcaaggttg tacattgcca aa 22 <210> 89 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M45 F Primer of Cucumis sativus L. <400> 89 ttcatattga cacaacttaa accttg 26 <210> 90 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M45 R Primer of Cucumis sativus L. <400> 90 gaggcttaca cctctcatag aag 23 <210> 91 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M46 F Primer of Cucumis sativus L. <400> 91 catgcatgga gatcatatat attttg 26 <210> 92 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M46 R Primer of Cucumis sativus L. <400> 92 acaaaatgga gctttgtttt gagaa 25 <210> 93 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M47 F Primer of Cucumis sativus L. <400> 93 tgctaaggaa ggtgggtgac aa 22 <210> 94 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M47 R Primer of Cucumis sativus L. <400> 94 tcatgggaga atgtgggtaa caa 23 <210> 95 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M48 F Primer of Cucumis sativus L. <400> 95 tttctggtgg gagagttgtg ttg 23 <210> 96 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M48 R Primer of Cucumis sativus L. <400> 96 tctctcatcc catttcaacc tca 23 <210> 97 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M49 F Primer of Cucumis sativus L. <400> 97 tgggaataga aaaactccga acaa 24 <210> 98 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M49 R Primer of Cucumis sativus L. <400> 98 ttgcctataa ggacggagtt tcaa 24 <210> 99 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M50 F Primer of Cucumis sativus L. <400> 99 gcatttcttc tgttattttt atttttcat 29 <210> 100 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> M50 R Primer of Cucumis sativus L. <400> 100 gctttgtgct cactgcttat g 21 <210> 101 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M51 F Primer of Cucumis sativus L. <400> 101 aactgagatg atgatgtgac aatcaaa 27 <210> 102 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M51 R Primer of Cucumis sativus L. <400> 102 acaacaagtt caccatcttt agtcgat 27 <210> 103 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M52 F Primer of Cucumis sativus L. <400> 103 caacattata ttcatttctt caaagtta 28 <210> 104 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M52 R Primer of Cucumis sativus L. <400> 104 ggtaagtgat gaaggtatag gtg 23 <210> 105 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M53 F Primer of Cucumis sativus L. <400> 105 cataagattc ttttgcttca ccattc 26 <210> 106 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M53 R Primer of Cucumis sativus L. <400> 106 ctccaaatga tattatgatt cacatg 26 <210> 107 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M54 F Primer of Cucumis sativus L. <400> 107 tgaagaaatc ggcgatggag 20 <210> 108 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> M54 R Primer of Cucumis sativus L. <400> 108 cccacactgc ggatccaac 19 <210> 109 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M55 F Primer of Cucumis sativus L. <400> 109 gtattttcca attcaaatca tattcttac 29 <210> 110 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M55 R Primer of Cucumis sativus L. <400> 110 acatattacg tagaaatgtg attgga 26 <210> 111 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M56 F Primer of Cucumis sativus L. <400> 111 tcccagaagt caatatccta gtgct 25 <210> 112 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M56 R Primer of Cucumis sativus L. <400> 112 ccctgtgttt ctgtttgagg aa 22 <210> 113 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M57 F Primer of Cucumis sativus L. <400> 113 tccaaatagc ttgtatgaaa ctctgtca 28 <210> 114 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M57 R Primer of Cucumis sativus L. <400> 114 tccattcagg accaatcttg tca 23 <210> 115 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M58 F Primer of Cucumis sativus L. <400> 115 gcatgataat aatctcaact ctgtta 26 <210> 116 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M58 R Primer of Cucumis sativus L. <400> 116 tgcaataggc tgaagaaatt cg 22 <210> 117 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M59 F Primer of Cucumis sativus L. <400> 117 gcctctttgc gttgggtcat 20 <210> 118 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M59 R Primer of Cucumis sativus L. <400> 118 tcctaaaacc tatcatggat ctga 24 <210> 119 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M60 F Primer of Cucumis sativus L. <400> 119 gcacgaagga agacaaaagt cg 22 <210> 120 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M60 R Primer of Cucumis sativus L. <400> 120 ccagtcgcca ccatctaagc 20 <110> Dongguk University Industry-Academic Cooperation Foundation <120> DNA Polymorphism Marker for Identification of Cucumis sativus L. <160> 120 <170> KopatentIn 1.71 <210> 1 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M1 F Primer of Cucumis sativus L. <400> 1 catttgaaag ttcaaccctt ctaaaca 27 <210> 2 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M1 R Primer of Cucumis sativus L. <400> 2 cctaaacttt gaaaagtaac aatttactc 29 <210> 3 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M2 F Primer of Cucumis sativus L. <400> 3 aatcctgaag ttcatgttga tgtgg 25 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M2 R Primer of Cucumis sativus L. <400> 4 ctctctaggc cataaactat cctc 24 <210> 5 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M3 F Primer of Cucumis sativus L. <400> 5 tgttccactc tacctatgac attttga 27 <210> 6 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M3 R Primer of Cucumis sativus L. <400> 6 accaaatgta tatgtttttc tttgcattt 29 <210> 7 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M4 F Primer of Cucumis sativus L. <400> 7 ttgtttacac cgtgtcagta acttaag 27 <210> 8 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> M4 R Primer of Cucumis sativus L. <400> 8 cgtgtgtaaa aattaaattc aaaacatttc 30 <210> 9 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M5 F Primer of Cucumis sativus L. <400> 9 ttttcgagca atcaaatggt ttttattta 29 <210> 10 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M5 R Primer of Cucumis sativus L. <400> 10 tgctactttc ttgagatgaa actttttg 28 <210> 11 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M6 F Primer of Cucumis sativus L. <400> 11 tgattgtgac tttgcaagga aatg 24 <210> 12 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M6 R Primer of Cucumis sativus L. <400> 12 tcttaagaga tggcccaacc aa 22 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M7 F Primer of Cucumis sativus L. <400> 13 ttgctgaatg caccaatgga 20 <210> 14 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M7 R Primer of Cucumis sativus L. <400> 14 ccatggaagg tattgagaaa ttgatg 26 <210> 15 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M8 F Primer of Cucumis sativus L. <400> 15 tcttaattaa ccgagagaca cttcc 25 <210> 16 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M8 R Primer of Cucumis sativus L. <400> 16 tcgttaaatt tgtatacgaa aaatttgat 29 <210> 17 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> M9 F Primer of Cucumis sativus L. <400> 17 aatctttact tgtatcaatc ttctaaattc 30 <210> 18 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M9 R Primer of Cucumis sativus L. <400> 18 caatttaaac ctcaaaactt ctttaaag 28 <210> 19 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M10 F Primer of Cucumis sativus L. <400> 19 tgaataaata ttttaaggtc gtatacag 28 <210> 20 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M10 R Primer of Cucumis sativus L. <400> 20 tccaccaaac cacactactc tt 22 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M11 F Primer of Cucumis sativus L. <400> 21 cgtctgtgct atacgccaag 20 <210> 22 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M11 R Primer of Cucumis sativus L. <400> 22 ttcaagcact taaagataag ccaa 24 <210> 23 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M12 F Primer of Cucumis sativus L. <400> 23 tatagaagat tagaagaaat acgttg 26 <210> 24 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M12 R Primer of Cucumis sativus L. <400> 24 aaccttacta gggtttacat tcgttt 26 <210> 25 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M13 F Primer of Cucumis sativus L. <400> 25 aaacgtgaag taatgagacc tagcgta 27 <210> 26 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M13 R Primer of Cucumis sativus L. <400> 26 catttgtaaa catcaattcg ccaaa 25 <210> 27 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M14 F Primer of Cucumis sativus L. <400> 27 tctctgtgtt ggtataacgt acca 24 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M14 R Primer of Cucumis sativus L. <400> 28 caagctaagt gagaatgtaa gtg 23 <210> 29 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M15 F Primer of Cucumis sativus L. <400> 29 tacagaattt gtgtttaaat tatttgtt 28 <210> 30 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M15 R Primer of Cucumis sativus L. <400> 30 atgtgatcaa atacatttat aaagg 25 <210> 31 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M16 F Primer of Cucumis sativus L. <400> 31 cttatatatt gttgatcaag ctattg 26 <210> 32 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M16 R Primer of Cucumis sativus L. <400> 32 gcacttacga ggataaacaa aatac 25 <210> 33 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M17 F Primer of Cucumis sativus L. <400> 33 aggtgcattt gggtagaatg ttg 23 <210> 34 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M17 R Primer of Cucumis sativus L. <400> 34 cagtgattta tttttgcacg gaact 25 <210> 35 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M18 F Primer of Cucumis sativus L. <400> 35 tccaaaaaga tcaattaact caagtctca 29 <210> 36 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M18 R Primer of Cucumis sativus L. <400> 36 ccggtccatc aactactact caagg 25 <210> 37 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> M19 F Primer of Cucumis sativus L. <400> 37 gccccaactc gctcgatg 18 <210> 38 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M19 R Primer of Cucumis sativus L. <400> 38 tgaaataaga tcacaaaaag gggc 24 <210> 39 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M20 F Primer of Cucumis sativus L. <400> 39 gcatacattg aaatgcaaca actca 25 <210> 40 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M20 R Primer of Cucumis sativus L. <400> 40 tcccctttag atgtccaata aatgc 25 <210> 41 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M21 F Primer of Cucumis sativus L. <400> 41 ttgatgaaga agcgtttaca gtatgg 26 <210> 42 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M21 R Primer of Cucumis sativus L. <400> 42 agtggatttg aaacgcaagg at 22 <210> 43 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M22 F Primer of Cucumis sativus L. <400> 43 tggccttaca attgctattt tcca 24 <210> 44 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M22 R Primer of Cucumis sativus L. <400> 44 gccttttcct attcgacttt tgtct 25 <210> 45 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M23 F Primer of Cucumis sativus L. <400> 45 aaagttaact catcgcaaaa ttgttg 26 <210> 46 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M23 R Primer of Cucumis sativus L. <400> 46 ggccaaaagt attagtccgt ag 22 <210> 47 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M24 F Primer of Cucumis sativus L. <400> 47 tcaaagtatt cattaagaaa attgcat 27 <210> 48 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M24 R Primer of Cucumis sativus L. <400> 48 tgaaaggggg ttagttttta aaga 24 <210> 49 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M25 F Primer of Cucumis sativus L. <400> 49 gttggttaaa tatatttttt tacaacttg 29 <210> 50 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M25 R Primer of Cucumis sativus L. <400> 50 aaacagacaa cttttctaat atacg 25 <210> 51 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M26 F Primer of Cucumis sativus L. <400> 51 tggcttcttg ccagattcca tt 22 <210> 52 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M26 R Primer of Cucumis sativus L. <400> 52 tctcttgaaa gttcctttga gatct 25 <210> 53 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M27 F Primer of Cucumis sativus L. <400> 53 aagaactgac tataaagatt aatttta 27 <210> 54 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M27 R Primer of Cucumis sativus L. <400> 54 taactcttag acatgtgagt atac 24 <210> 55 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M28 F Primer of Cucumis sativus L. <400> 55 ctaatatgga atgaaaaaca aggttg 26 <210> 56 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M28 R Primer of Cucumis sativus L. <400> 56 ttaactctta gacatgtgag tatact 26 <210> 57 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M29 F Primer of Cucumis sativus L. <400> 57 caacatcaac atgaccccat aa 22 <210> 58 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M29 R Primer of Cucumis sativus L. <400> 58 ttgcactgga agttctgaaa agg 23 <210> 59 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M30 F Primer of Cucumis sativus L. <400> 59 ctgagaaaaa tggcggcaag 20 <210> 60 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M30 R Primer of Cucumis sativus L. <400> 60 gggacattgt cgatgagatt ga 22 <210> 61 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M31 F Primer of Cucumis sativus L. <400> 61 gcgattttaa tcaaaaattt cagtg 25 <210> 62 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M31 R Primer of Cucumis sativus L. <400> 62 tcttccaata tactctctcc ct 22 <210> 63 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M32 F Primer of Cucumis sativus L. <400> 63 tgcaatgtgt cattccccat tta 23 <210> 64 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M32 R Primer of Cucumis sativus L. <400> 64 ccctatacct ctacattcat ctc 23 <210> 65 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M33 F Primer of Cucumis sativus L. <400> 65 tgtaatttgc cattttaaga accttga 27 <210> 66 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M33 R Primer of Cucumis sativus L. <400> 66 gaaggaaaat tggttggaat ttcg 24 <210> 67 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M34 F Primer of Cucumis sativus L. <400> 67 catgttttta ataattaaaa ataaatcat 29 <210> 68 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M34 R Primer of Cucumis sativus L. <400> 68 atgataaata ataactttta gttcg 25 <210> 69 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M35 F Primer of Cucumis sativus L. <400> 69 tgatcgtagt tattctccct tcaa 24 <210> 70 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> M35 R Primer of Cucumis sativus L. <400> 70 gaaaaaggac taaaaatgtg aataaataag 30 <210> 71 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M36 F Primer of Cucumis sativus L. <400> 71 catcgctttc aaaagaggtt gg 22 <210> 72 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M36 R Primer of Cucumis sativus L. <400> 72 gaacctccaa atgggattca gg 22 <210> 73 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M37 F Primer of Cucumis sativus L. <400> 73 tgcaatcaca gaaatggaac cag 23 <210> 74 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M37 R Primer of Cucumis sativus L. <400> 74 tcaacttttg atgcaaactg atgg 24 <210> 75 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M38 F Primer of Cucumis sativus L. <400> 75 cgcgaagaag aagaggcaga 20 <210> 76 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M38 R Primer of Cucumis sativus L. <400> 76 ccctagcttt gtgcgacgtt 20 <210> 77 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M39 F Primer of Cucumis sativus L. <400> 77 cagtcgctgg gccttctttc 20 <210> 78 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M39 R Primer of Cucumis sativus L. <400> 78 ttcaggagac aaagcgagac ctt 23 <210> 79 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M40 F Primer of Cucumis sativus L. <400> 79 tattggatat ccacattgtg acag 24 <210> 80 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M40 R Primer of Cucumis sativus L. <400> 80 ggatttctgt tttgatggat agaac 25 <210> 81 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M41 F Primer of Cucumis sativus L. <400> 81 gtaattaaat accacataat atcacaa 27 <210> 82 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M41 R Primer of Cucumis sativus L. <400> 82 tgttcaagag gctatacatc cta 23 <210> 83 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M42 F Primer of Cucumis sativus L. <400> 83 ggattccaaa gaatttgttc tcca 24 <210> 84 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M42 R Primer of Cucumis sativus L. <400> 84 gccttatggg tttaaattca atactc 26 <210> 85 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M43 F Primer of Cucumis sativus L. <400> 85 atagatatgt acgtcgattt ctcca 25 <210> 86 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M43 R Primer of Cucumis sativus L. <400> 86 taggctttat attttggatt aagttg 26 <210> 87 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M44 F Primer of Cucumis sativus L. <400> 87 ctttcaagaa ctattaacca ttgc 24 <210> 88 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M44 R Primer of Cucumis sativus L. <400> 88 cgcaaggttg tacattgcca aa 22 <210> 89 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M45 F Primer of Cucumis sativus L. <400> 89 ttcatattga cacaacttaa accttg 26 <210> 90 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M45 R Primer of Cucumis sativus L. <400> 90 gaggcttaca cctctcatag aag 23 <210> 91 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M46 F Primer of Cucumis sativus L. <400> 91 catgcatgga gatcatatat attttg 26 <210> 92 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M46 R Primer of Cucumis sativus L. <400> 92 acaaaatgga gctttgtttt gagaa 25 <210> 93 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M47 F Primer of Cucumis sativus L. <400> 93 tgctaaggaa ggtgggtgac aa 22 <210> 94 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M47 R Primer of Cucumis sativus L. <400> 94 tcatgggaga atgtgggtaa caa 23 <210> 95 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M48 F Primer of Cucumis sativus L. <400> 95 tttctggtgg gagagttgtg ttg 23 <210> 96 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M48 R Primer of Cucumis sativus L. <400> 96 tctctcatcc catttcaacc tca 23 <210> 97 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M49 F Primer of Cucumis sativus L. <400> 97 tgggaataga aaaactccga acaa 24 <210> 98 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M49 R Primer of Cucumis sativus L. <400> 98 ttgcctataa ggacggagtt tcaa 24 <210> 99 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M50 F Primer of Cucumis sativus L. <400> 99 gcatttcttc tgttattttt atttttcat 29 <210> 100 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> M50 R Primer of Cucumis sativus L. <400> 100 gctttgtgct cactgcttat g 21 <210> 101 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M51 F Primer of Cucumis sativus L. <400> 101 aactgagatg atgatgtgac aatcaaa 27 <210> 102 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> M51 R Primer of Cucumis sativus L. <400> 102 acaacaagtt caccatcttt agtcgat 27 <210> 103 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M52 F Primer of Cucumis sativus L. <400> 103 caacattata ttcatttctt caaagtta 28 <210> 104 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M52 R Primer of Cucumis sativus L. <400> 104 ggtaagtgat gaaggtatag gtg 23 <210> 105 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M53 F Primer of Cucumis sativus L. <400> 105 cataagattc ttttgcttca ccattc 26 <210> 106 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M53 R Primer of Cucumis sativus L. <400> 106 ctccaaatga tattatgatt cacatg 26 <210> 107 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M54 F Primer of Cucumis sativus L. <400> 107 tgaagaaatc ggcgatggag 20 <210> 108 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> M54 R Primer of Cucumis sativus L. <400> 108 cccacactgc ggatccaac 19 <210> 109 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> M55 F Primer of Cucumis sativus L. <400> 109 gtattttcca attcaaatca tattcttac 29 <210> 110 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M55 R Primer of Cucumis sativus L. <400> 110 acatattacg tagaaatgtg attgga 26 <210> 111 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> M56 F Primer of Cucumis sativus L. <400> 111 tcccagaagt caatatccta gtgct 25 <210> 112 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M56 R Primer of Cucumis sativus L. <400> 112 ccctgtgttt ctgtttgagg aa 22 <210> 113 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> M57 F Primer of Cucumis sativus L. <400> 113 tccaaatagc ttgtatgaaa ctctgtca 28 <210> 114 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> M57 R Primer of Cucumis sativus L. <400> 114 tccattcagg accaatcttg tca 23 <210> 115 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> M58 F Primer of Cucumis sativus L. <400> 115 gcatgataat aatctcaact ctgtta 26 <210> 116 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M58 R Primer of Cucumis sativus L. <400> 116 tgcaataggc tgaagaaatt cg 22 <210> 117 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M59 F Primer of Cucumis sativus L. <400> 117 gcctctttgc gttgggtcat 20 <210> 118 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> M59 R Primer of Cucumis sativus L. <400> 118 tcctaaaacc tatcatggat ctga 24 <210> 119 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> M60 F Primer of Cucumis sativus L. <400> 119 gcacgaagga agacaaaagt cg 22 <210> 120 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M60 R Primer of Cucumis sativus L. <400> 120 ccagtcgcca ccatctaagc 20
Claims (4)
서열번호 83과 84의 올리고뉴클레오티드로 구성되는 M42 프라이머 세트; 및
서열번호 93과 94의 올리고뉴클레오티드로 구성되는 M47 프라이머 세트;로 이루어진 군으로부터 선택되는 하나 이상의 프라이머 세트;를 포함하는 오이 품종 식별용 마커 조성물.M6 primer set consisting of oligonucleotides of SEQ ID NOs: 11 and 12;
M42 primer set consisting of oligonucleotides of SEQ ID NOs: 83 and 84; And
M47 primer set consisting of oligonucleotides of SEQ ID NO: 93 and 94; at least one primer set selected from the group consisting of; marker composition for identifying cucumber varieties.
(b) 상기 분리된 게놈 DNA를 주형으로 하고, 서열번호 11과 12의 올리고뉴클레오티드로 구성되는 M6 프라이머 세트; 서열번호 83과 84의 올리고뉴클레오티드로 구성되는 M42 프라이머 세트; 및 서열번호 93과 94의 올리고뉴클레오티드로 구성되는 M47 프라이머 세트;로 이루어진 군으로부터 선택되는 하나 이상의 프라이머 세트;를 이용하여 게놈 DNA를 증폭하는 단계; 및
(c) 상기 증폭된 산물을 분석하여 오이의 품종을 식별하는 단계를 포함하는 오이 품종을 식별하는 방법.(a) isolating genomic DNA derived from cucumbers;
(b) a set of M6 primers comprising the isolated genomic DNA as a template and consisting of oligonucleotides of SEQ ID NOs: 11 and 12; M42 primer set consisting of oligonucleotides of SEQ ID NOs: 83 and 84; And amplifying genomic DNA using at least one primer set selected from the group consisting of M47 primer set consisting of oligonucleotides of SEQ ID NOs: 93 and 94; And
(c) analyzing the amplified product to identify a cucumber variety.
서열번호 83과 84의 올리고뉴클레오티드로 구성되는 M42 프라이머 세트; 및
서열번호 93과 94의 올리고뉴클레오티드로 구성되는 M47 프라이머 세트;로 이루어진 군으로부터 선택되는 하나 이상의 프라이머 세트;를 포함하는 오이 품종 식별용 키트.M6 primer set consisting of oligonucleotides of SEQ ID NOs: 11 and 12;
M42 primer set consisting of oligonucleotides of SEQ ID NOs: 83 and 84; And
Kit for identifying cucumber varieties comprising; M47 primer set consisting of oligonucleotides of SEQ ID NO: 93 and 94; one or more primer set selected from the group consisting of.
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