KR20130059128A - MATERIAL AS AGONIST FOR PPARα, PPARγ AND PPARδ USING DENDROPANAX MORBIFERA LEV. LEAF AND THE EXTRACTING METHOD THEREOF - Google Patents

Abstract

PURPOSE: PPAR alpha, gamma, and delta active materials using Dendropanax morbifera Lev. leaves are provided to prevent or treat diseases such as obesity, diabetes, hyperlipidemia, hypertension, atherosclerosis, cardiovascular diseases, metabolic diseases, cancer, and inflammation, and to be used as a main ingredient of a pharmaceutical product, a functional food, and a health food. CONSTITUTION: A method for isolating PPAR alpha, gamma, and delta active materials using Dendropanax morbifera Lev. leaves comprises: a step of extracting Dendropanax morbifera Lev. leaves with hydrous alcohol and preparing concentrate(S101); and a step for fractioning the concentrate with a non-polar solvent(S103). The PPAR alpha, gamma, and delta active materials contain a Dendropanax morbifera Lev. leaf extract and a fraction thereof. The PPAR alpha, gamma, and delta active materials further contains a pharmaceutically acceptable inactive carrier. The inactive carrier is selected from a group consisting of starch and lactose. [Reference numerals] (AA) Start; (BB) End; (S101) Concentration step of extracting Dendropanax morbifera Lev. leaves with hydrous alcohol and preparing concentrate; (S103) Fraction preparing step for fractioning the concentrate obtained from the concentration step with a non-polar solvent

Description

PARA R, γ, and δ active substances using the leaves of Hilchi chinensis and methods for extracting the same. LEAF AND THE EXTRACTING METHOD THEREOF}

The present invention relates to PPAR α, γ, and δ active substances using the leaves of Dendropanax morbifera Lev., And a method of extracting the same, and more particularly, to enhance the activity of PPAR α, γ, and δ that regulates homeostasis of glucose. The present invention relates to PPAR α, γ, and δ active substances useful in medicines, functional foods, and food additives for treating and preventing hyperglycemia associated with insulin resistance and diabetes, and extracting methods thereof.

The present invention relates to a PPAR α, γ and δ active substance using the hwangchil tree leaves and more particularly, the method of extracting the insulin, and more particularly the action of increasing the activity of PPAR α, γ and δ to regulate the homeostasis of glucose The present invention relates to PPAR α, γ and δ active substances useful as medicines, functional foods and food additives for the treatment and prevention of hyperglycemia associated with resistance and diabetes, and methods of extracting the same.

Peroxisome proliferator activated receptors (PPARs) regulate the expression of genes primarily involved in fat metabolism or genes involved in the differentiation of adipocytes (J. Invest. Dermatol. 111, 1116-1121, 1998; J. Med Chem., 43, 527-550, 2000), which refers to an intranuclear receptor whose ligand is a peroxisome proliferator, which is a compound capable of increasing the number of peroxisomes, PPARα, PPARδ, Isoforms of PPARγ are known (J. Steroid Biochem. Molec. Biol., 51, 157, 1994; Gene Expression, 4, 281, 1995; Biochem. Biophys. Res. Commun., 224, 431, 1996 ).

Among the isoforms of PPAR, PPARα is mainly expressed in liver, retina, and adipose tissue, and is involved in fatty acid oxidation or neutralization of toxic substances and in inflammatory reactions. PPARγ is mainly expressed in adipocytes, immune cells, adrenal gland, spleen, and small intestine, and is a transcription factor activated by fatty acids. It is an important regulator of adipocyte differentiation and function. In addition, the glucose tolerance of adipocytes is maximized, adipocyte differentiation is promoted and insulin resistance is reduced (Tren. Pharmacol. Sci., 25: 331-336, 2004). In addition, since PPARγ plays a role in regulating glucose homeostasis, PPARγ activators have been developed as potent drugs for treating insulin resistance and hyperglycemia associated with type 2 diabetes, and are currently pioglitazone and Thiazolidinediones such as rosiglitazone have been used as therapeutic agents for diabetes as a representative ligand of PPARγ. In other words, the activation of PPARγ increases the availability of glucose in the muscle, inhibits the synthesis of glucose in the liver, and increases cholesterol outflow from macrophages, resulting in insulin sensitization, glucose lowering, and triglyceride lowering effects. Treat Diabetes

It has already been found in the literature that PPARγ activators have been shown to be effective in the treatment of insulin resistance and hyperglycemia associated with type 2 diabetes [1) Diabetes 45: 1661-1669, 1996; 2) J. Clin. Invest. 106: 467-472, 2000; 3) Diabetes 51: 797-802, 2002; 4) Diabetologia 48: 83-95, 2005].

On the other hand, PPARδ is not tissue-specifically expressed, but is widely expressed and its function is unclear (Endocrinology., 137, 354, 1996), but later PPARδ activity increased the concentration of HDL cholesterol in db / db mice. It has been suggested that PPARδ activators, particularly when administered to insulin-resistant middle-aged obese rhesus monkeys that cause significant dose dependence, lower the concentration of low-density LDL, fix triglycerides, and fix insulin at the same time. Raise serum HDL cholesterol (PNAS 98: 5306-5311, 2001). In addition to effects on cholesterol homeostasis, PPARδ treatment has been observed to lower plasma glucose and improve insulin sensitivity in diabetic ob / ob mice and high fat diets that lead to insulin resistance mice (PNAS 100: 15924-15929, 2003). These observations suggest that PPARδ activation is useful for the treatment and prevention of cardiovascular diseases and diseases including type 2 diabetes and atherosclerosis, hypertriglyceridemia and mixed dyslipidemia (WO 01/00603).

As a sole approach, glucose lowering is thought to be limited in overcoming the macrovascular complications associated with type 2 diabetes and metabolic syndrome, so treatment of type 2 diabetes and metabolic syndrome not only lowers the apparent hypertriglyceridemia associated with these syndromes, but also hyperglycemia. Should be aimed at mitigating. This suggests that studies on compounds exhibiting varying levels of PPARδ activity include type 2 diabetes, dyslipidemia, and metabolic syndrome (including glucose tolerance, insulin resistance, hypertriglyceridemia and / or obesity), cardiovascular diseases (including atherosclerosis) And the discovery of triglycerides and / or cholesterol and / or glucose lowering agents that are likely to be used in the treatment of diseases such as hypercholesterolemia.

The prevalence of diabetes in Korea was estimated to be less than 1% in 1970, reaching 5% in the 2000s. According to the 2010 National Health Insurance Corporation's medical expenses data, the number of diabetes patients was 20,15,180, or 1.63 million in 2006. The number of patients increased by 23.9%, which is emerging as a social problem due to the westernization of dietary and aging problems.

Diabetes is a disease in which blood sugar rises due to lack of insulin or inoperability, and progresses to chronic vascular complications if not actively treated. Among the diabetic patients, type 2 diabetes related to lifestyle, such as overeating or lack of exercise, is associated with insulin secretion disorder and insulin resistance, and type 1 is associated with autoimmune factor and pancreatic beta cell destruction.

Type 2 diabetes is the leading type of insulin resistance. The main etiologies are obesity, decreased insulin receptors, reduced tyrosine kinase activity, decreased muscle / adipose tissue type transporters in muscle and adipose tissue, and insulin receptor substrate-1 (IRS). -1) has been reported to be caused by complex causes such as intracellular deficiency. Type 2 diabetes occurs mainly in adults and accounts for 90% of all diabetes (Drugs, 65: 433-445, 2005).

On the other hand, a diet rich in triglycerides and free fatty acids is known to be a cause of metabolic diseases such as obesity, type 2 diabetes, heart disease, hypertension. But on the other side of the two-sided fatty acids play an important role. That is, fatty acids are stored in adipocytes as triglycerides.In this case, patients with lipodystrophies who have inherently no adipocytes have severe metabolic diseases such as insulin resistance, hyperglycemia, and hyperlipidemia. I'm sick. Therefore, fat cells and fatty acids are essential components in maintaining health. The study of adipocytes has made many achievements, among which the discovery of nuclear hormone receptors known as PPARα, PPARγ and PPARδ is a remarkable result.

Based on these findings, studies on activating PPARs from natural herbal medicines have recently been made, and there are examples of substances that increase the activity of PPARγ in plants such as brier mushrooms and nutmegs (domestic patent registration 10-0760999, 10-0959557). However, as the side effects of thiazolidinedione drugs that activate only PPARγ have been raised, questions such as rosiglitazone have been withdrawn from the market, raising questions about drugs that activate only PPARγ. In the case of natural herbal medicines, considering the presence of various active substances, the development of drugs without multiple side effects by activating three types of PPARs will be a remarkable development direction.

Hwangchil-tree is an evergreen broad-leaved arboreous tree that grows only in the southwestern coastal area of Jeollanam-do and Jeju Island, which belongs to the Armenaceae family.

Hwangchil has been used as a rare paint that emits the golden color of emperor's armor, helmets, and other metal ornaments since the Three Kingdoms.It is a Korean envoy, written in the Goryeo Dynasty, and Guilin History, Guilin, and Haedong History. It is written on the back, and it remains in the Tangbu History Book Book District, which is a special product of Baekje.

However, not only Hwangchil tree extract but also Hwangchil tree are effective in eliminating heat, detoxifying alcohol, treating eye and jaundice, treating burns, and leprosy and are harmless to the human body (Lee, Jin-jin, Herb, Chinese Moonshine Book, 1590). It has been known to exhibit sedation and tonic action (research of unique global agricultural commodities (Hwang Chil-pyeon), Jeollanam-do, 1996). In addition, it is known that the leaf extract fraction has anti-cancer activity and slightly weaker antioxidant activity than α-tocopherol (Patent Publication No. 2000-0004499, Park Ho-geun et al., 1998), but it has been used for thousands of years. Considering the value, the results of pharmacological activity are very poor.

Dendropanoxide and polyacetylene compounds have also been found in ingredient studies (Journal of Ethnopharmacology 90, 403-408 (2004) Park Bo-young, etc.). Considering the reality that has been used in various diseases, there is almost no scientific explanation. Recently, there have been some reports that dendropanoxide has some effect in animal experiments of streptozotocin-induced type 1 diabetes model (Human and Experimental Toxicology (2010)), and the relationship between specific component-pharmacological activity It can be said that it is not done.

Against this background, the present inventors collected natural herbal medicines that are known to be effective in diabetes in folks that activate PPARα, PPARγ, and PPARδ to overcome insulin resistance, which is the mainstream of diabetes, and metabolic diseases caused by type 2 diabetes. During the investigation, it was confirmed that the extract of Hwangchil-tree leaves and its organic solvent fractions increased three kinds of PPAR activity. In particular, the activity of PPARδ showed more excellent effects. The complex activity of the visible PPARs has been accomplished by confirming that no activity has been reported so far.

As these three PPAR isoforms play an important role in fat metabolism with respect to blood glucose lowering in vivo, in the present invention, the degree of activation of these PPAR isoforms is examined in order to detect the hypoglycemic action of the herbal extracts derived from Hwangchil-tree. Looking to develop a comprehensive treatment for metabolic disorders, in particular, it is excellent in increasing the activity of PPARγ and PPARδ that regulates glucose homeostasis, and thus, drugs and functionalities for treating and preventing hyperglycemia associated with insulin resistance and diabetes. Provided are PPAR α, γ, and δ active substances using yellow lacquer leaves useful as foods or food additives, and methods for extracting the same.

An object of the present invention is a PPAR α, γ, and δ active substance using the leaves of Hilchi chinensis which is used as a main raw material for the treatment and prevention of hyperglycemia, functional foods and health foods that exhibit hypoglycemic effects associated with insulin resistance and diabetes. It is to provide an extraction method.

An object of the present invention is a PPAR α using a yellow lacquer leaves, characterized in that the extract comprises the step of extracting the sulfuric acid leaves with a hydrous alcohol to prepare a concentrate and the fraction obtained in the concentration step fractions with a non-polar solvent, by providing a method for extracting γ and δ actives.

According to a preferred feature of the present invention, the step of concentrating is prepared by adding Hwangchil-tree leaves to hydrous alcohol and refluxing for 5 to 24 hours at a temperature of 90 to 100 ° C to produce Hwangchil-tree leaves extract, After cooling to 50 DEG C, the supernatant of the filtrate obtained by filtration is separated, and then the separated supernatant is concentrated by evaporation.

According to a more preferred feature of the present invention, the concentration step is to add the yellow lacquer leaves to the water-containing alcohol and cold soaked for 5 to 7 days at a temperature of 4 to 20 ℃ to prepare the yellow lacquer leaves extract, After separating the supernatant by filtration, the separated supernatant shall be evaporated and concentrated.

According to a more preferred feature of the invention, the fractionation step is to be made by dividing the concentrate obtained in the concentration step into a separatory filter with a non-polar solvent having 4 to 6 carbon atoms.

According to a further preferred feature of the invention, the nonpolar solvent is said to be normal hexane.

In addition, the object of the present invention can also be achieved by providing PPAR α, γ and δ active material using the hwangchil leaves, characterized in that consisting of the hwangchil tree leaves extract and the hwangchil tree leaves extract.

According to a preferred feature of the present invention, the PPAR α, γ and δ active material using the yellow lacquer leaves will further include a pharmaceutically acceptable inert carrier.

According to a further preferred feature of the invention, the inert carrier is to be made of one or more selected from the group consisting of starch and lactose.

According to a more preferable feature of the present invention, the PPAR α, γ and δ active material using the yellow lacquer leaves are at least one additive selected from the group consisting of a diluent, a flavoring agent, a solubilizer, a lubricant, a suspending agent, a binder and a boric acid agent. It shall be included more.

PPAR α, γ and δ active substance and extracting method using the Hwangchil leaves according to the present invention prevent or treat diseases such as obesity, diabetes, hyperlipidemia, hypertension, arteriosclerosis, cardiovascular diseases, metabolic diseases, cancer and inflammation It exhibits an excellent effect of providing PPAR α, γ and δ active substances which can be used as main ingredients of medicines, functional foods and health foods.

1 is a flow chart illustrating a method of extracting PPAR α, γ and δ active material using the Hwangchil tree leaves according to the present invention.
Figure 2 is a graph showing the PPAR α activation effect of the Hwangchil-tree leaves extract prepared through Examples 1 to 6 of the present invention and its fractions.
Figure 3 is a graph showing the PPAR-γ activation effect of the Hwangchil-tree leaves extract prepared through Examples 1 to 6 of the present invention and fractions thereof.
Figure 4 is a graph showing the PPAR-δ activation effect of the Hwangchil-tree leaves extract prepared through Examples 1 to 6 of the present invention and fractions thereof.

Hereinafter, preferred embodiments of the present invention and physical properties of the respective components will be described in detail with reference to the accompanying drawings. However, the present invention is not limited thereto, And this does not mean that the technical idea and scope of the present invention are limited.

Extraction method of PPAR α, γ and δ active material using the Hwangchil tree leaves according to the present invention is to extract the Hwangchil tree leaves with water-containing alcohol to prepare a concentrated solution (S101) and the concentrate obtained in the concentrated step (S101) Fraction preparation step of fractionating with a non-polar solvent (S103).

The step of concentrating (S101) is a step of preparing a concentrate by extracting the yellow lacquer tree leaves with water-containing alcohol, by adding hot yellow leaf to water-containing alcohol and refluxing for 5 to 24 hours at a temperature of 90 to 100 ° C. After preparing the extract, and cooling the Hwangchil-tree extract to 20 to 50 ℃ and separating the supernatant of the filtrate obtained by filtration, the separated supernatant is made by evaporation or concentrated, or the Hilchi chile leaves into hydrated alcohol After chilling for 5 to 7 days at a temperature of 4 to 20 ℃ to prepare the Hwangchil-tree leaf extract, and after filtering the Hwangchil-tree leaf extract to separate the supernatant, the separated supernatant is concentrated by evaporation, concentrate In order to increase the yield of the active ingredient contained in the concentrated step (S101) three times by repeating the supernatant obtained in each concentration step to the final concentrated solution It is preferable to use a method to collect.

In the two concentration steps (S101) as described above, a hydrous alcohol is used as an extraction solvent, which can be extracted using water in addition to the hydrous alcohol, and the alcohol used in the hydrous alcohol has a concentration of 10 to 100% methanol or Ethanol can be used.

The concentrate obtained through the concentration step may be obtained in a liquid state with fluidity, but may also be obtained in a dry powder state using a lyophilization method.

The fraction production step (S103) is a step of fractionating the concentrate obtained in the concentration step (S101) with an organic solvent, the concentrate obtained in the concentration step (S101) is formed by dividing with a solvent in a separatory filter, the degree of polarity In order to obtain a fraction according to the above, the liquid or dried powder concentrate was extracted and filtered with an organic solvent three times, and repeated three times to add the filtrates obtained in each extraction and filtration step to obtain a final fraction. It is preferable to use a collecting method.

At this time, the organic solvent is not particularly limited as long as it can fractionate the concentrate obtained in the concentration step (S101), hexane, chloroform, ethyl acetate, butanol and water, etc. may be used, the fraction is PPAR α, γ and δ In order to have an excellent activity against, it is preferable to use a nonpolar solvent of alkanes having 4 to 10 carbon atoms, and more preferably to use normal hexane.

PPAR α, γ and δ active material using the Hwangchil tree leaves according to the present invention is composed of a fraction of Hwangchil tree leaf extract and Hwangchil tree leaf extract.

At this time, the fractions of the Hwangchil-tree leaf extract and the Hwangchil-tree leaf extract are extracts and fractions extracted using the method of extracting PPAR α, γ and δ active substances using the yellow-lacquer leaves, and the components and roles are the same. Description thereof will be omitted.

In addition, the PPAR α, γ and δ active material using the yellow lacquer tree leaf may further include a pharmaceutically acceptable inert carrier, wherein the inert carrier orally uses the PPAR α, γ and δ active material using the yellow lacquer tree leaf. It serves to formulate pharmaceutical preparations in solid, semi-solid or liquid form suitable for administration, and pharmaceutically acceptable inert carriers which can be suitably used for this purpose can be solid or liquid, from the group consisting of starch and lactose It is preferred to consist of one or more selected.

In addition, the PPAR α, γ and δ active material using the yellow lacquer leaves may further include one or more additives selected from the group consisting of a diluent, a flavoring agent, a solubilizer, a lubricant, a suspending agent, a binder, and a boric acid agent.

In this case, the PPAR α, γ, and δ active material formulated into pharmaceutical preparations in solid, semi-solid or liquid form with the carrier added may be prepared as tablets, capsules, and syrups. It is obtained by sieving the fractions fractionated through the preparation step, mixing with lactose, starch and pregelatinized corn starch, granulating into powder by adding purified water, drying the granules, mixing with magnesium stearate and pressing. Preferred ingredients and contents of the tablet are 5.0 mg of PPAR α, γ and δ actives, 150.0 mg of lactose BP, 30.0 mg of starch BP, 15.0 mg of pregelatinized corn starch BP and 1.0 mg of magnesium stearate.

In addition, the capsule is prepared by sieving the fraction fractionated through the fraction production step and then mixed with excipients and filled into gelatin capsules, the preferred components and content of the capsule is PPAR α, γ and δ active material 5.0 mg, 100.0 mg of starch (1500) and 1.0 mg of magnesium stearate BP.

In addition, the syrup is dissolved in sucrose in 500 ml of purified water, and dissolved in carboxymethyl cellulose sodium in 400 ml of purified water in a separate container, and then the purified water in which the white sugar is dissolved and purified water in which methyl cellulose sodium is dissolved are mixed. And propylparaben are added to dissolve, and after adding ethanol, purified water is added so that the total solution has a volume of 1000 ml, and the PPAR α, γ, and δ active substances fractionated through the fraction preparation step are suspended therein. Preferred components and contents of the syrup are 5.0 g of PPAR α, γ and δ active substances, 637.5 g per bag, 2.0 g of sodium carboxymethylcellulose, 0.28 g of methylparaben, 0.12 g of propylparaben, 20 ml of ethanol, and the remaining amount of purified water.

In addition, the PPAR α, γ and δ active material using the hwangchil tree leaves excellent blood sugar lowering effect when used for the purpose of treating diabetes, it is preferable to administer 0.0001 to 0.10g compared to 1kg of the weight of the subject, The dosage is not particularly limited and may vary depending on the needs of the patient, the extent of the condition to be treated and the components and contents in the mixture used together.

Hereinafter, the method of extracting PPAR α, γ, and δ active substances using the Hwangchil tree leaves according to the present invention and the effect of PPAR α, γ, and δ of the extracted active substance will be described with reference to Examples.

≪ Example 1 >

The process of taking 100 g of yellow chile leaves into 500 ml of methanol solution (methanol: water = 4: 1) at a suitable size, cooling them at room temperature for 3 days, and filtering them with multiple layers of gauze to obtain the filtrate supernatant. Repeated three times, the supernatants obtained through three iterations were combined, and the combined supernatants were depressurized with a rotary evaportor to completely evaporate water and methanol to extract the yellow lacquer leaf extract as a powder.

<Example 2>

After extracting the powdered Hwangchil-tree leaf extract prepared in Example 1 into a separatory filter at room temperature with hexane (normal hexane) for 24 hours, filtering the filter with multiple layers of filter paper and then taking the supernatant three times. , And the supernatants obtained through three iterations were combined, and the combined supernatants were decompressed with a rotary evaportor to completely remove the solvent hexane, thereby fractionating the yellow lacquer leaves.

<Example 3>

Proceed in the same manner as in Example 2, using the chloroform instead of hexane to fractionate the Hwangchil wood leaf extract.

<Example 4>

Proceed in the same manner as in Example 2, using the ethyl acetate instead of hexane to fractionate the Hwangchil wood leaf extract.

<Example 5>

Proceed in the same manner as in Example 2, butanol instead of hexane was used to fractionate the hwangchil wood leaf extract.

<Example 6>

Proceed in the same manner as in Example 2, using the water instead of hexane to fractionate the yellow lacquer leaves.

<Experimental Example 1>

The activation effects of PPAR α, γ, and δ of the Hwangchil-tree leaf extract and fractions obtained through Examples 1 to 6 were measured and shown in FIGS. 2 to 4 below.

Renal cells CV-1 cells were incubated in DMEM medium containing 10% calf serum (FBS) at 5% carbon dioxide, 37 ° C. The cells were inoculated with 6 × 10 4 cells per well in 48 well plates for use in this experiment. Incubated for 24 hours. Lipofectamine plasmid DNA of luciferase activity reporter DNA (tk-PPREx3-luc), PPAR-α (pCMX-mPPARa), PPAR-δ (pCMX-mPPARd) and PPAR-γ (pCMX-mPPARg) Transformation was performed at 37 ° C. for 7 hours using LTX reagent and PLUS reagent. 400 μl each in a cell culture system using dimethyl sulfoxide (DMSO, dimethyl sulfoxide) as a solvent to concentrate 1.0 to 500 μg / ml concentration of the Hwangchil-tree leaf extract obtained through Examples 1 to 4 and the fraction thereof. After treatment and incubation for 40 hours, the culture solution was removed, and 50 µl of lysis buffer was added to lyse the cells while shaking for 20 minutes. 20 μl of cell lysate and 50 μl of luciferase assay reagent were added thereto, and the luminescence value was measured by a luminometer.

At this time, 0.1% dimethyl sulfoxide was used as the negative control group, 10 μm for the PPARα agonist GW7647, 10 μm for the PPARδ agonist GW0742, and 10 μg for the PPARγ agonist pioglitazone, respectively. The experimental results were expressed as a% Max value representing the value of the sample relative to the maximum value of the positive control group, the% Max value is calculated by the following equation (1).

&Quot; (1) &quot;

% Max = 100 × (sample activity-negative control activity) / (positive control activity-negative control activity)

As shown in Figures 2 to 4 below, it can be seen that the fractions of the Hwangchil-tree leaf extract and the Hwangchil-tree leaf extract exhibit excellent activity on PPAR α, γ, and δ, and the hwangchil exhibiting excellent activity on PPAR α, γ, and δ The active substance consisting of the fractions of the tree leaf extract and the yellow lacquer tree leaf extract shows excellent activity against PPAR α, γ and δ.

In particular, it can be seen that the fraction fractionated through Example 2 shows the best activity against PPAR α, γ and δ.

S101: concentration step
S103: fraction production step

Claims (9)

Concentrating step of producing a concentrate by extracting the hwangchil tree leaves with a hydrous alcohol; And
A method for extracting PPAR α, γ, and δ active substances using the yellow lacquer leaf, characterized in that the fraction preparation step of fractionating the concentrate obtained in the concentration step with a non-polar solvent;
The method according to claim 1,
In the concentration step, the yellow lacquer tree leaves are poured into a hydrous alcohol and heated to reflux for 5 to 24 hours at a temperature of 90 to 100 ° C. to produce a yellow lacquer tree leaf extract, and the yellow lacquer tree extract is cooled to 20 to 50 ° C. and then filtered. And then separating the supernatant of the filtrate obtained by evaporation, followed by evaporating and concentrating the separated supernatant.
The method according to claim 1,
In the concentration step, the yellow lacquer leaves are added to the hydrous alcohol, and then cooled for 5 to 7 days at a temperature of 4 to 20 ° C. to prepare the yellow lacquer leaves extract, and after filtering the yellow lacquer leaves extract to separate the supernatant, A method for extracting PPAR α, γ, and δ active substances using a yellow lacquer leaf, characterized by evaporating and separating the separated supernatant.
The method according to claim 1,
The fractionation step is a method for extracting PPAR α, γ and δ active material using the yellow lacquer leaves, characterized in that the concentrated solution obtained in the concentration step is put into a separatory filter and fractionated with a non-polar solvent having 4 to 6 carbon atoms.
The method according to claim 1,
The non-polar solvent is a method for extracting PPAR α, γ and δ active material using the hwangchil leaves, characterized in that the normal hexane.
PPAR α, γ and δ active material using the hwangchil leaves, characterized in that consisting of the extract of the Hwangchil-tree leaves and the Hilchi-chile leaves extract.
The method of claim 6,
PPAR α, γ and δ active material using the yellow lacquer leaves PPAR α, γ and δ active material using a yellow lacquer tree, characterized in that the pharmaceutically acceptable inert carrier further comprises.
The method of claim 7,
The inert carrier is PPAR α, γ and δ active material using the yellow lacquer tree, characterized in that consisting of one or more selected from the group consisting of starch and lactose.
The method of claim 6,
PPAR α, γ and δ active material using the hwangchil leaves further comprises one or more additives selected from the group consisting of a diluent, a flavoring agent, a solubilizer, a lubricant, a suspending agent, a binder and a boric acid agent PPAR α, γ and δ active substances using leaves.

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