KR20130051846A - Method for controlling plant growth and increasing antioxidant enzyme using led light - Google Patents
Method for controlling plant growth and increasing antioxidant enzyme using led light Download PDFInfo
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- KR20130051846A KR20130051846A KR1020110117230A KR20110117230A KR20130051846A KR 20130051846 A KR20130051846 A KR 20130051846A KR 1020110117230 A KR1020110117230 A KR 1020110117230A KR 20110117230 A KR20110117230 A KR 20110117230A KR 20130051846 A KR20130051846 A KR 20130051846A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
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- F21K—NON-ELECTRIC LIGHT SOURCES USING LUMINESCENCE; LIGHT SOURCES USING ELECTROCHEMILUMINESCENCE; LIGHT SOURCES USING CHARGES OF COMBUSTIBLE MATERIAL; LIGHT SOURCES USING SEMICONDUCTOR DEVICES AS LIGHT-GENERATING ELEMENTS; LIGHT SOURCES NOT OTHERWISE PROVIDED FOR
- F21K9/00—Light sources using semiconductor devices as light-generating elements, e.g. using light-emitting diodes [LED] or lasers
- F21K9/20—Light sources comprising attachment means
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
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- F21W—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES F21K, F21L, F21S and F21V, RELATING TO USES OR APPLICATIONS OF LIGHTING DEVICES OR SYSTEMS
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- F21W2131/10—Outdoor lighting
- F21W2131/109—Outdoor lighting of gardens
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
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Abstract
Description
본 발명은 LED광을 이용한 식물 성장 조절방법 및 항산화효소 증대 방법에 관한 것이다.The present invention relates to a method for controlling plant growth and an antioxidant enzyme increasing method using LED light.
LED (Light Emitting Diode)는 발광다이오드라 하며, 이러한 광에너지 기술은 사용자가 원하는 짧은 파장을 구현 할 수 있어 파장 선택이 용이하고, 소비전력이 낮고 고효율과 긴수명으로 인하여 인공광원으로 세계 각국에서 이용되고 있다. 최근 심각한 기후 변화로 급격한 온도 변화, 사막화와 호우 등이 세계적으로 큰 문제가 되고 있으며, 이에 농산물의 생산량에 막대한 손실을 가져와 안정적 농산물 공급이 중요시되고 있다. LED (Light Emitting Diode) is a light emitting diode, and this light energy technology can realize short wavelength that user wants, so it is easy to select wavelength, low power consumption, high efficiency and long life, so it is used as artificial light source in various countries around the world. It is becoming. In recent years, due to severe climate change, rapid temperature changes, desertification and heavy rain have become a big problem in the world, and this has caused a huge loss in the production of agricultural products, and thus stable supply of agricultural products is important.
따라서, 본 발명이 해결하고자 하는 과제는 LED 광원을 이용하여, 농산물의 성장을 조절하여, 생산을 증대시키는 방법을 제공하는 것이다. Therefore, the problem to be solved by the present invention is to provide a method of increasing the production by controlling the growth of agricultural products, using an LED light source.
상기 과제를 해결하기 위하여, 본 발명은 LED 광을 이용한 식물 성장 조절방법으로, 상기 방법은 청색광, 녹색광 또는 적색광의 LED 광을 선택하여, 식물의 성장을 조절하는 것을 특징으로 한다. In order to solve the above problems, the present invention is a plant growth control method using the LED light, the method is characterized in that to control the growth of the plant by selecting the LED light of blue light, green light or red light.
본 발명의 일 실시예에 따르면, 상기 방법은 상기 LED 광이 적색광 또는 녹색광인 경우, 상기 식물의 길이 생장이 촉진된다. 또한, 상기 LED 광이 청색광인 경우, 상기 식물의 조직이 견고해진다. According to one embodiment of the present invention, the method promotes the length growth of the plant when the LED light is red light or green light. In addition, when the LED light is blue light, the texture of the plant becomes firm.
본 발명은 또한 LED 광을 이용한 식물의 항산화 효소 증가 방법으로, 상기 방법은 청색 단파장 LED 광을 식물에 조사하는 단계를 포함하는 것을 특징으로 하는 LED 광을 이용한 식물의 항산화 효소 증대 방법을 제공한다. The present invention also provides a method for increasing the antioxidant enzyme of a plant using LED light, the method provides a method for increasing the antioxidant enzyme of a plant using LED light, wherein the method includes irradiating the plant with blue short wavelength LED light.
본 발명의 일 실시예에 따르면, 상기 청색광은 460nm의 파장대를 갖는다.According to an embodiment of the present invention, the blue light has a wavelength band of 460 nm.
본 발명에 따르면, 청색광, 녹색광 또는 적색광의 LED 광을 선택하여, 식물의 성장을 선택적으로 조절한다. 따라서, 재배되는 농산물의 종류에 따른 자유로운 성장 조절이 가능하며, 그 결과 별도의 화학합성 농약의 사용없이 농산물의 생산이 증대될 수 있다. 아울러, 청색 LED광을 식물에 조사함에 따라 상기 식물로부터 생산되는 농산물 내의 항산화효소가 증대된다. According to the present invention, the LED light of blue light, green light or red light is selected to selectively control the growth of plants. Therefore, it is possible to freely control growth according to the type of agricultural products grown, and as a result, the production of agricultural products can be increased without the use of a separate chemical synthetic pesticide. In addition, as the blue LED light is irradiated to plants, antioxidant enzymes in agricultural products produced from the plants are increased.
도 1은 파장별 토마토의 길이 및 가지수, 잎 등 외형적 비교결과,
도 2는 파장별 토마토의 줄기 생장 정도 비교 분석 결과(A:혼합광, B:청색광, C: 적색광, D: 녹색광),
도 3은 파장별 proline 함량 비교결과,
도 4는 파장별 DPPH free radical 소거능 비교결과,
도 5는 파장별 폴리페놀 화합물 비교결과,
도 6은 Superoxide dismutase (SOD) 효소 활성 분석결과,
도 7은 Catalase (CAT) 효소 활성 분석결과,
도 8은 Peroxidase (POX) 효소 활성 분석결과,
도 9는 Ascorbate peroxidase (APX) 효소 활성 분석결과, 및
도 10은 Glutathione reductase (GR) 효소 활성 분석결과이다. 1 is a comparison result of the appearance of the length and number of tomatoes, leaves, etc.
2 is a comparative analysis result of the stem growth degree of tomatoes for each wavelength (A: mixed light, B: blue light, C: red light, D: green light),
3 is a comparison result of the proline content for each wavelength,
4 is a comparison result of DPPH free radical scavenging ability by wavelength,
5 is a comparison result of polyphenol compounds for each wavelength,
Figure 6 shows the results of Superoxide dismutase (SOD) enzyme activity assay,
7 is Catalase (CAT) enzyme activity assay results,
8 is a Peroxidase (POX) enzyme activity assay results,
9 shows Ascorbate peroxidase (APX) enzyme activity assay results, and
10 shows the results of Glutathione reductase (GR) enzyme activity analysis.
이하, 본 발명의 바람직한 실시예를 첨부된 도면들을 참조하여 상세히 설명한다. 우선 각 도면의 구성요소들에 참조부호를 부가함에 있어서, 동일한 구성요소들에 대해서는 비록 다른 도면상에 표시되더라도 가능한 동일한 부호를 가지도록 하고 있음에 유의하여야 한다. 또한, 하기에서 본 발명을 설명함에 있어, 관련된 공지기능 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략한다. Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. First, in adding reference numerals to the components of each drawing, it should be noted that the same reference numerals are used to refer to the same components, even if displayed on different drawings. In addition, in the following description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.
본 발명에 따른 실험예는 하기와 같다. 하지만, 하기에 설명되는 실험방법, 장치 등은 모두 본 발명을 예시하기 위한 것으로, 본 발명의 범위가 하기 예에 제한되지 않는다. Experimental examples according to the invention are as follows. However, all experimental methods, apparatuses, and the like described below are intended to illustrate the present invention, and the scope of the present invention is not limited to the following examples.
본 발명은 하기 예시되는 실시예에서 토마토를 각각의 LED 단색광 생장상 조건에서 20일 생육시킨 뒤 형태적으로 크게 차이를 보인 초장, 가지 수, 가지 당 잎수, 잎 너비, 가지 길이 등 5종의 주요 형태학적 특성들을 비교 분석하였고, 각각의 LED 단색광 처리에 대한 토마토 식물의 생장은 대조구(백색광)와 비교하였다. According to the present invention, after the tomato was grown for 20 days in each LED monochromatic light growing condition in the following examples, the five main types of plants, branches, leaves per branch, leaf width, and branch length showed significant differences in shape. Morphological characteristics were analyzed and the growth of tomato plants for each LED monochromatic treatment was compared with the control (white light).
이를 통하여, 본 발명은 청색광은 1.0배, 적색광은 1.6배, 그리고 녹색광에서는 2.0배의 길이 생장을 보였다. 또한, 가지 길이에서 적색광과 녹색광이 대조구와 청색광 보다 1.4 ~ 1.6배 더 높았던 반면에 가지 수, 가지 당 잎수, 잎 너비는 모든 광에서 유사한 경향을 보였다. 이상의 각 LED 단일광 조건에서의 토마토 생육 조사를 종합해보면, 대조구(백색광)와 비교하여 적색와 녹색광 조건에서는 길이 생장이 약 2배 정도 큰 반면 줄기 조직이 가늘고 약한 생육 상태를 보였다. 그러나 청색광 조건에서 생장한 토마토는 대조구(백색광)와 유사하게 줄기 조직이 매우 견고하고 단단하였으며 강한 생육상태를 보였다. 따라서, 본 발명에 따른 LED광의 파장대를 녹색, 적색, 청색으로 선택적으로 이용하여, 식물의 생장과 생육 상태를 효과적으로 제어할 수 있다. 즉, 생장의 경우에는 녹색광이나, 적색광을 사용하고, 줄기 조직을 견고하게 성장시키는 경우에는, 청색광을 사용할 수 있다. 특히 청색광의 경우에는 식물의 생장과 함께 줄기조직의 견실함을 동시에 달성할 수 있다는 장점이 있다. 본 발명의 일 실시예에서 청색광은 400nm 내지 550nm의 파장대를 가지며, 이 중 460nm의 단파장을 갖는 것이 바람직하다.
Through this, in the present invention, the blue light showed 1.0 times, the red light 1.6 times, and the green light showed a length of 2.0 times. In addition, the red and green light at branch lengths were 1.4-1.6 times higher than the control and blue light, while the number of branches, number of leaves per branch, and leaf width showed similar trends in all the lights. In summary, the growth of tomato under the single light condition of LEDs showed that the length and growth of the stem tissue were thin and weak in the red and green light conditions compared to the control (white light). However, the tomato grown under the blue light condition showed very strong and firm stem tissue and strong growth similar to the control (white light). Therefore, by selectively using the wavelength band of the LED light according to the present invention in green, red, and blue, it is possible to effectively control the growth and growth state of the plant. That is, green light or red light is used for growth, and blue light can be used for firmly growing stem tissue. In particular, in the case of blue light, there is an advantage that the growth of the plant and the robustness of the stem tissue can be achieved at the same time. In one embodiment of the present invention blue light has a wavelength range of 400nm to 550nm, of which the short wavelength of 460nm is preferred.
이하 본 발명에 따른 식물 성장 제어방법을 실험예로 상세히 설명한다. 하지만, 하기 실험예는 모두 본 발명을 설명하기 위한 것으로, 본 발명의 범위가 하기 실험예에 의하여 제한되지 않는다.
Hereinafter, the plant growth control method according to the present invention will be described in detail as an experimental example. However, all of the following experimental examples are for explaining the present invention, and the scope of the present invention is not limited by the following experimental examples.
여러 가지의 단파장을 활용한 식물 생장 및 형태적 비교Plant Growth and Morphological Comparison Using Various Short Wavelengths
토마토 종자 100립을 선종하여 70% 에탄올에 1분 동안 표면소독한 후 2% sodium hypochloride에 20분 동안 종자 소독을 한 뒤, 수돗물과 증류수로 각각 10회 이상 세척하였다. 세척된 종자를 150 x 20 mm petri dish에 여지를 2장씩 깔고, 수분을 충분히 공급한 후 종자를 치상하여 25℃ 암 상태로 3일 동안 최아시켰다. 정상적으로 균일하게 최아 된 종자를 멸균된 상토가 담긴 pot에 파종하고 2 주일정도 자연광에서 생장시킨 후 LED 생장상 안으로 옮겨 주었다. LED 생장상 조건은 25± 1℃, 습도 50~55%, 그리고 광파장대는 백색 (대조구, 420 ~ 680nm), 청색 (460nm), 적색 (635nm), 녹색 (520nm)이고, 광량대는 1000 LUX로 일정하게 해주었다. 광조사시간은 15 h(광조건)/9 h(암조건)의 광주기 상태에서 생장을 진행하였다. 100 tomato seeds were adenosed, surface sterilized in 70% ethanol for 1 minute, seed sterilized in 2% sodium hypochloride for 20 minutes, and washed 10 times or more with tap water and distilled water. The washed seeds were placed in a 150 x 20 mm petri dish with two sheets of paper, supplied with sufficient moisture, and seeded and seeded at 25 ° C for 3 days. Normally, evenly sprouted seeds were sown in pots containing sterilized top soil, grown in natural light for about two weeks, and then transferred into LED growth phases. LED growth conditions are 25 ± 1 ℃, humidity 50 ~ 55%, light wavelength is white (control, 420 ~ 680nm), blue (460nm), red (635nm), green (520nm), and light intensity is constant at 1000 LUX I let you. Light irradiation time was 15 h (light condition) / 9 h (dark condition) in the photoperiod state of growth.
도 1은 파장별 토마토의 길이 및 가지수, 잎 등 외형적 비교결과이다. 1 is a comparison result of the appearance of the length and number of branches, leaves, etc. for each wavelength.
도 1을 참조하면, 청색광은 1.0배, 적색광은 1.6배, 그리고 녹색광에서는 2.0배의 길이 생장을 보였다. 가지 길이에서 적색광과 녹색광이 대조구와 청색광 보다 1.4 ~ 1.6배 더 높았던 반면에 가지 수, 가지 당 잎수, 잎 너비는 모든 광에서 유사한 경향을 보였다. Referring to FIG. 1, blue light showed 1.0 times, red light 1.6 times, and green light 2.0 times in length. In branch length, red and green light showed 1.4 ~ 1.6 times higher than control and blue light, whereas the number of branches, number of leaves per branch, and leaf width showed similar tendency in all light.
파장별By wavelength 유관속Flow 분석 analysis
가장 큰 형태적 특성을 보인 줄기 조직을 양날 면도칼을 이용하여 횡/종단면을 최대한 얇게 자른 다음 petri-dish에 증류수를 받아 그 위에 잘린 표본들을 모은다. 가장 잘 된 표본을 슬라이드글라스 위에 올린 뒤 아세트산카민 염색약을 한 방울 떨어 뜨려 20 ~ 30분 동안 염색한다. 커버글라스를 덮고 주변의 용액을 흡습지로 제거한 후 microscope (Eclipse E200, Nikon, Japan)을 이용하여 각 LED 단색광별 토마토 줄기 유관속 조직의 형태를 관찰하였다. Using the double-blade razor, the stem tissue with the greatest morphological characteristics is cut as thinly as possible, and the specimens are collected by distilled water on petri-dish. The best specimen is placed on a slide glass, and then stained with a drop of carbohydrate acetate dye for 20 to 30 minutes. After covering the cover glass and removing the surrounding solution with the absorbent paper, the shape of the tomato stem flow tube tissue for each LED monochromatic light was observed using a microscope (Eclipse E200, Nikon, Japan).
도 2는 파장별 토마토의 줄기 생장 정도 비교 분석 결과(A:혼합광, B:청색광, C: 적색광, D: 녹색광)이다.2 is a result of comparative analysis of the stem growth degree of tomatoes for each wavelength (A: mixed light, B: blue light, C: red light, D: green light).
도 2를 참조하면, 대조구(백색광)와 비교하여 적색와 녹색광 조건에서는 길이 생장이 약 2배 정도 큰 반면 줄기 조직이 가늘고 약한 생육 상태를 보였다. 그러나, 청색광 조건에서 생장한 토마토는 대조구(백색광)와 유사하게 줄기 조직이 매우 견고하고 단단하였으며 강한 생육상태를 보였다. Referring to Figure 2, compared to the control (white light) in the red and green light conditions, the length of the growth is about 2 times larger, the stem tissue showed a thin and weak growth state. However, the tomato grown under the blue light condition showed very strong and firm stem tissue and strong growth similar to the control (white light).
따라서, 이상의 도 1과 도 2의 결과를 참조하면, 본 발명에 따른 LED광의 파장대를 녹색, 적색, 청색으로 선택적으로 이용하여, 식물의 생장과 생육 상태를 효과적으로 제어할 수 있다. 즉, 생장의 경우에는 녹색광이나, 적색광을 사용하고, 줄기 조직을 견고하게 성장시키는 경우에는, 청색광을 사용할 수 있다. 특히 청색광의 경우에는 식물의 생장과 함께 줄기조직의 견실함을 동시에 달성할 수 있다는 장점이 있다.
Therefore, referring to the results of FIGS. 1 and 2, the growth and growth state of the plant can be effectively controlled by selectively using the wavelength band of the LED light according to the present invention in green, red, and blue. That is, green light or red light is used for growth, and blue light can be used for firmly growing stem tissue. In particular, in the case of blue light, there is an advantage that the growth of the plant and the robustness of the stem tissue can be achieved at the same time.
단색광Monochromatic light 처리 후 항산화 효소 분석 및 기타 물질 분석 Analysis of antioxidant enzymes and other substances after treatment
- Proline 함량 분석-Proline content analysis
많은 식물 종에서 proline의 축적은 stress-조절 유전자의 발현을 자극하는 대표적인 삼투제로서 스트레스 내성과 관련되어 있으며, stress 조건에서 sub-cellular 구조(막과 단백질)를 안정화시키고 free-radicals를 소거하며, 세포질의 산화 환원을 완충해 주는 기능을 가지고 있다. 따라서 생육조사에서 형태적으로 큰 차이를 보인 토마토 식물의 잎과 줄기에서 각 LED 단일광 처리에 따른 proline 함량을 비교 조사하였다. 이를 위하여, 본 실험예에서는 토마토 식물(작물)은 발아 일주일 후 각각의 단색광별 LED 생장상 조건에서 20일 생육시킨 후 잎과 줄기의 proline 함량을 측정하여 비교 분석하였다. Proline 함량 분석은 Bates 등(1973)의 방법으로 추출하여 분석하였다. 각 시료 0.5g을 취해 10 ml MCW (methanol : chloroform : water = 12 : 5 : 1, v/v/v)액을 첨가한 뒤 균질화 시키고, 상온에서 12,000 rpm으로 10분간 원심분리 한다. 상징액 2 ml을 2 ml acid-ninhydrin and 2 ml glacial acetic acid가 혼합된 용액과 혼합하여 1시간 동안 100℃에서 끓인 뒤, 즉시 5 ~ 10분 동안 ice-bath로 옮겨 반응을 중지시킨다. 반응이 중지된 혼합액에 4 ml toluene을 첨가하여 20초 동안 voltexing 하여 잘 혼합하고, 상온에서 30분간 정치 한 뒤 UV-spectrophotometer (UV-1800, Shimadzu Corp., Japan)을 이용하여 520nm에서 흡광도를 측정하여 proline 함량을 분석하였다.In many plant species, proline accumulation is associated with stress tolerance as a representative osmotic agent that stimulates expression of stress-regulated genes, stabilizes sub-cellular structures (membrane and proteins) and eliminates free-radicals under stress conditions, It has a function to buffer the redox of the cytoplasm. Therefore, we investigated the proline contents of the leaves and stems of tomato plants, which showed morphological differences in growth. To this end, in the present experimental example, the tomato plants (crops) were grown for 20 days under the conditions of LED growth for each monochromatic light one week after germination, and then measured by comparing the proline contents of leaves and stems. Proline content analysis was performed by extraction by Bates et al. (1973). Take 0.5 g of each sample, add 10 ml MCW (methanol: chloroform: water = 12: 5: 1, v / v / v) solution, homogenize and centrifuge for 10 minutes at 12,000 rpm at room temperature. 2 ml of the supernatant is mixed with a solution containing 2 ml acid-ninhydrin and 2 ml glacial acetic acid, boiled at 100 ° C for 1 hour, and then immediately transferred to an ice-bath for 5 to 10 minutes to stop the reaction. 4 ml toluene was added to the mixed solution to stop the reaction and mixed well for 20 seconds. After standing at room temperature for 30 minutes, absorbance was measured at 520 nm using a UV-spectrophotometer (UV-1800, Shimadzu Corp., Japan). Proline content was analyzed.
도 3은 파장별 proline 함량 비교결과이다. 3 is a comparison result of the proline content for each wavelength.
도 3을 참조하면, 각각의 LED 단색광 처리에 대한 토마토 식물의 proline 함량은 대조구(백색광)와 비교해 잎과 줄기 모두 청색광 조건에서 현저하게 높은 경향을 보였다.
Referring to FIG. 3, the proline content of the tomato plants for each LED monochromatic light treatment tended to be significantly higher in the blue and light conditions of both the leaves and the stems compared to the control (white light).
- DPPH free radical 소거 능력 분석-DPPH free radical scavenging ability analysis
토마토 식물(작물)은 발아 일주일 후 각각의 단색광별 LED 생장상 조건에서 20일 생육시킨 후 잎과 줄기의 DPPH radical 소거 능력을 측정하여 비교 분석하였다. DPPH (1,1-diphenyl-2-picrylhydrazyl)를 기질로 이용하여 radical scavenging 활성을 측정하는 Blois (1958)의 방법에 따라 0.2 mM DPPH methanol 용액을 흡광도가 2.0이 되도록 조절하여 분획시료 150 ul와 DPPH methanol 750 ul를 혼합하여 25℃에서 30분간 반응시킨 다음 UV-spectrophotometer (UV-1800, Shimadzu Corp., Japan)을 이용하여 517nm에서 흡광도를 측정하였다. 측정된 흡광도는 다음 식에 의해 DPPH free radical 소거 활성 값으로 환산하였다. Tomato plants (crops) were grown for 20 days under monochromatic LED growth conditions one week after germination, and then analyzed by comparing DPPH radical scavenging ability of leaves and stems. According to Blois's (1958) method, which measures radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) as a substrate, 150 mM ul and DPPH were fractionated by adjusting the absorbance of 0.2 mM DPPH methanol to 2.0. After mixing 750 ul of methanol and reacting at 25 ° C. for 30 minutes, absorbance was measured at 517 nm using a UV-spectrophotometer (UV-1800, Shimadzu Corp., Japan). The absorbance measured was converted into DPPH free radical scavenging activity value by the following equation.
도 4는 파장별 DPPH free radical 소거능 비교결과이다. 4 is a comparison result of DPPH free radical scavenging ability according to wavelengths.
도 4를 참조하면, 각각의 LED 단색광 처리에 대한 토마토 식물의 DPPH free radical 소거 능력은 대조구(백색광)와 비교해 잎과 줄기 모두 청색광 조건에서 다소 높은 경향을 보였다.
Referring to FIG. 4, the DPPH free radical scavenging ability of the tomato plant for each LED monochromatic light treatment tended to be somewhat higher in the blue and light conditions of both the leaves and the stems compared to the control (white light).
- 폴리페놀 화합물 함량 분석-Polyphenol Compound Content Analysis
토마토 식물(작물)은 발아 일주일 후 각각의 단색광별 LED 생장상 조건에서 20일 생육시킨 후 잎과 줄기의 총 폴리페놀 화합물 함량을 측정하여 비교 분석하였다. 총 폴리페놀 화합물 함량의 분석은 Singleton and Rossi (1965)의 방법에 따라 Folin-Ciocalteau reagent(F-C 시약)가 알칼리 조건에서 추출물의 polyphenol성 화합물에 의해 환원된 결과 노란색에서 몰리브덴 청색으로 발색되는 원리로 UV-spectrophotometer (UV-1800, Shimadzu Corp., Japan)을 이용하여 740nm에서 총 폴리페놀의 양을 측정하여 비교분석하였다. Tannic acid를 이용한 표준곡선은 gallic acid의 최종농도가 0, 100, 150, 250, 500 ug/ml이 되도록 하여 740nm에서 흡광도를 측정하여 작성하였다. Tomato plants (crops) were grown for 20 days under the conditions of LED growth for each monochromatic light one week after germination, and then analyzed by comparing total polyphenol compound contents of leaves and stems. The analysis of the total polyphenolic compound content is based on the principle that the Folin-Ciocalteau reagent (FC reagent) is colored yellow to molybdenum blue as a result of the reduction of the polyphenolic compound of the extract under alkaline conditions by the method of Singleton and Rossi (1965). The total amount of polyphenols was measured at 740 nm using a spectrophotometer (UV-1800, Shimadzu Corp., Japan). Standard curve using tannic acid was prepared by measuring the absorbance at 740 nm with final concentration of gallic acid at 0, 100, 150, 250, 500 ug / ml.
도 5는 파장별 폴리페놀 화합물 비교결과이다. 5 is a comparison result of polyphenol compounds for each wavelength.
도 5를 참조하면, 항산화 관련 물질인 폴리페놀 화합물과 항산화관련 효소들을 조사한 결과 총 폴리페놀 화합물 함량은 대조구(백색광)와 비교해 잎과 줄기 모두 청색광 조건에서 상당히 높은 경향을 보였다.
Referring to FIG. 5, as a result of investigating the antioxidant-related polyphenol compounds and antioxidant enzymes, the total polyphenol compound content was significantly higher in both blue and blue leaves compared to the control (white light).
- 항산화효소활성 비교 분석-Comparative analysis of antioxidant enzyme activity
토마토 식물(작물)은 발아 일주일 후 각각의 단색광별 LED 생장상 조건에서 20일 생육시킨 후 잎과 줄기의 항산화효소의 활성을 측정하여 비교 분석하였다. 전체 Superoxide dismutase (SOD) 활성은 Oberley와 Spitz (1984)의 방법에 딸 nitro 청색 tetrazolium (NBT) 의존적 O2 -의 환원 억제 정도를 560nm에서 측정하여 검정하였다. Catalase (CAT)활성 분석은 Beers와 sizer의 방법(1952)에 따라 H2O2의 이하작용을 240nm에서 측정하여 검정하였다. Guaiacol peroxidase (POX)활성 분석은 Chance와 Maehly (1955)의 방법에 따라 H2O2가 존재하는 가운데 470nm에서 guaiacol이 tetraguaiacol을 형성하는 반응을 측정하여 검정하였다. Ascorbate peroxidase (APX)활성 분석은 Nkano and Asada (1981)의 방법에 딸 ascorbate가 산화되어 감소되는 양을 290nm에서 측정하여 검정하였다. Glutathione reductase (GR)활성 분석은 O′kane et al. (1996)방법에 따라 NADPH의 산화반응을 340nm에서 측정하여 검정하였다. Tomato plants (crops) were grown for 20 days under the conditions of LED growth for each monochromatic light one week after germination, and then analyzed by comparing antioxidant activities of leaves and stems. Total Superoxide dismutase (SOD) activity was assayed by measuring the inhibition of reduction of daughter nitro blue tetrazolium (NBT) dependent O 2 − at 560 nm using the method of Oberley and Spitz (1984). Catalase (CAT) activity assay was assayed by measuring H 2 O 2 subatmospheric activity at 240 nm according to Beers and sizer method (1952). Guaiacol peroxidase (POX) activity analysis was performed by measuring the reaction of guaiacol to form tetraguaiacol at 470 nm in the presence of H 2 O 2 according to the method of Chance and Maehly (1955). Ascorbate peroxidase (APX) activity assay was performed by Nkano and Asada (1981) to determine the amount of oxidized decrease in daughter ascorbate at 290 nm. Glutathione reductase (GR) activity analysis was performed by O'kane et al. According to the (1996) method, the oxidation reaction of NADPH was measured and assayed at 340 nm.
도 6 내지 10은 각각 Superoxide dismutase (SOD) 효소 활성 분석결과, Catalase (CAT) 효소 활성 분석결과, Peroxidase (POX) 효소 활성 분석결과, Ascorbate peroxidase (APX) 효소 활성 분석결과, Glutathione 적색uctase (GR) 효소 활성 분석결과이다. 6 to 10 shows the results of Superoxide dismutase (SOD) enzyme activity analysis, Catalase (CAT) enzyme activity analysis, Peroxidase (POX) enzyme activity analysis, Ascorbate peroxidase (APX) enzyme activity analysis, Glutathione reductase (GR) Results of enzyme activity analysis.
도 6 내지 10을 참조하면, SOD 활성은 대조구(백색광)와 비교해 잎에서는 모두 비슷한 수준이었으나 잎과 줄기 모두 청색광 조건에서 SOD 활성이 증가하였다. 또한 CAT 활성은 대조구(백색광)와 비교해 잎과 줄기 모두에서 적색와 녹색광 조건에서는 감소하였으나 청색광 조건에서만 다소 증가하였고, POX 활성은 청색광 조건에서는 잎과 줄기 모두 대조구(백색광)와 비슷한 수준을 유지하였다. 그러나 적색와 녹색광 조건에서는 잎의 POX 활성이 대조구 보다 각각 약 1.2배(117.6%)와 1.3배(133.0%)까지 높았다. 식물의 APX 활성은 대조구(백색광)와 비교해 잎과 줄기 모두에서 적색와 녹색광 조건에서는 감소하였으나 청색광 조건에서는 약간 증가하였으며, GR 활성은 대조구(백색광)와 비교해 잎과 줄기 모두에서 적색와 녹색광 조건에서는 감소하였으나 청색광 조건에서는 현저하게 증가하는 경향을 보여 항산화 물질 생산이 청색광에서 증가됨을 확인하였으며, 식물의 외형인 형태적인 측면에서도 견고해짐을 확인하였다.
6 to 10, the SOD activity was similar in both leaves compared to the control (white light), but the SOD activity was increased in both blue and blue leaves and stem conditions. In addition, CAT activity was decreased in both red and green light conditions in both leaves and stems compared to the control (white light), but slightly increased only in blue light conditions, and POX activity was similar to the control (white light) in blue light conditions. However, under red and green light conditions, the POX activity of the leaves was about 1.2 times (117.6%) and 1.3 times (133.0%) higher than the control, respectively. APX activity of plants decreased in both red and green light conditions in both leaves and stems compared to the control (white light) but slightly increased in blue light conditions. GR activity decreased in both red and green light conditions in both leaf and stem compared to the control (white light) but decreased in blue light. Under the conditions, it showed a tendency to increase significantly, and the production of antioxidants was confirmed to be increased in blue light, and it was confirmed that it was also robust in terms of morphology, which is the appearance of the plant.
Claims (5)
청색광, 녹색광 또는 적색광의 LED 광을 선택하여, 식물의 성장을 조절하는 것을 특징으로 하는 LED 광을 이용한 식물 성장 조절방법.Plant growth control method using the LED light, the method
Plant growth control method using LED light, characterized in that to control the growth of the plant by selecting the LED light of blue light, green light or red light.
상기 LED 광이 적색광 또는 녹색광인 경우, 상기 식물의 길이 생장이 촉진되는 것을 특징으로 하는 LED 광을 이용한 식물 성장 조절방법.The method of claim 1,
If the LED light is red light or green light, the growth of the plant length is characterized in that the plant growth control method using the LED light.
상기 LED 광이 청색광인 경우, 상기 식물의 조직이 견고해지는 것을 특징으로 하는 LED 광을 이용한 식물 성장 조절방법.The method of claim 2,
When the LED light is blue light, the plant growth control method using the LED light, characterized in that the plant tissue is firm.
상기 청색광은 460nm의 파장대를 갖는 것을 특징으로 하는 LED 광을 이용한 식물의 항산화 효소 증대 방법.5. The method of claim 4,
The blue light has a wavelength band of 460nm, the antioxidant enzyme enhancement method of plants using LED light.
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| JP2006304610A (en) * | 2005-04-26 | 2006-11-09 | Ccs Inc | Method and device for cultivating lettuce |
| KR20100057849A (en) * | 2007-08-15 | 2010-06-01 | 렘니스 라이팅 페이턴트 홀딩 비.브이. | Led lighting device for growing plants |
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