KR20120111118A - Composition for treatment of renal cell carcinoma and beauty expenses composition comprising extract of alpiniae officinari rhizoma - Google Patents

Abstract

PURPOSE: A composition and a cosmetic composition containing Alpiniae Officinari Rhizoma ethanol extract are provided to suppress pancreatic cancer cell proliferation and to treat a patient with renal cancer. CONSTITUTION: A composition for preventing and treating renal cancer contains Alpiniae Officinari Rhizoma ethanol extract as an active ingredient, prepared at 50 Deg. C. for 24 hours. The composition also contains pharmaceutically acceptable carriers or diluents. The renal cancer is kidney cell cancer. A cosmetic composition for preventing rental cancer contains Alpiniae Officinari Rhizoma ethanol extract containing cosmetologically acceptable cosmetic additives as an active ingredient. [Reference numerals] (AA) Relative survival rate

Description

Composition for the treatment of kidney cancer and cosmetic composition comprising a high amount river extract {Composition for Treatment of Renal Cell Carcinoma and Beauty Expenses Composition Comprising Extract of Alpiniae Officinari Rhizoma}

The present invention relates to a novel use of high amount river extract. Specifically, the present invention relates to a therapeutic composition and a cosmetic composition containing a high amount of the extract as an active ingredient exhibiting excellent prophylactic or therapeutic efficacy against kidney cancer.

Among the major diseases of modern people, researches on the treatment and diagnosis of cancer are being conducted mainly on lung cancer, liver cancer, and stomach cancer, which have high incidence.

The kidney is an important urinary system that has the role of excreting waste products in vitro by filtering blood to produce urine. At the same time, it is also an important endocrine organ that produces hormones such as angiotensin, which regulates blood pressure, and erythropoietin, a red blood cell hematopoietic factor. Renal tumors include kidney cell cancer in adults, Wilms' tumor in children, and sarcoma as a rare tumor, but the most common malignancy below Renal cell cancer, a tumor, is called kidney cancer. The incidence of renal cancer is about 2.5 per 100,000 population, and male to female ratio is 2 to 3: 1. Among urological malignancies, there are many tumors following prostate cancer and bladder cancer.

Genetic factors are also known as risk factors for kidney cancer, but generally include smoking and excessive fat intake. It is also known that the incidence of such tumors is high in patients undergoing long-term dialysis. In kidney cancer, when the tumor has a maximum diameter of 5 cm or less, some subjective symptoms rarely occur and are often found by CT scan or the like at the time of examination. Larger tumors may include hematuria, abdominal tumors, and pain. In addition, systemic symptoms may cause fever, weight loss, anemia and the like, and rarely, endocrine factors may cause erythrocytosis, hypertension, hypercalcemia, and the like. On the other hand, there may be cases in which vein veins of the abdominal body surface or venous varicose veins occur due to the progression of kidney cancer into the inferior vena cava. About 20% of kidney cancers are found from lung or bone metastases. In kidney cancer, tumors tend to spread in veins, and metastasis to other organs is likely to occur.

Ultrasound, CT, angiography, etc. are examined for kidney cancer, but specific biochemical markers are not known. Therefore, examination using a device is necessary. In addition, although several types of kidney cancer can be classified pathologically, the cause of the development of clear cell type kidney cancer, which accounts for about 90%, is known to be a defect in the von-Hippel-Lindau (VHL) gene, a cancer suppressor gene. . Deletion of the VHL gene results in transcriptional activation of the hypoxic inducible gene group through association of HIF-α / VHF, leading to enhanced expression of genes such as VEGF and TGFβ.

The main treatment for kidney cancer is surgical therapy. Regardless of the timing of the disease, extraction of the kidneys or partial extraction of the kidneys is the most common where possible, and even if there is metastasis, surgical removal of the kidneys may be considered. Arterial embolization of the renal artery may be used as a method other than the surgical therapy. This method may be performed prior to surgery in cases where it is impossible to extract or when a large tumor is removed. Recent advances in burn diagnostics have led to early detection of renal cancer, even at very small sizes, resulting in more than 90% healing in early cancer. However, since the therapeutic results of large tumors larger than 5 cm or tumors with metastases are poor (the 5-year survival rate of all renal cell carcinomas is about 50 to 60%), the importance of early detection is recognized.

On the other hand, the Alpinia officinarum Hance has an unusual aroma and a warm taste of adnau. It is effective for abdominal pain, vomiting and diarrhea caused by cold, and it is effective for weakness in kidney function, diarrhea due to cold symptoms, diarrhea and dysentery. Chronic gastritis, loss of appetite, and drink when the stool is not cool. Pharmacological action has been reported to inhibit the development of hemolytic streptococci, diphtheria bacteria and pneumococci.

High amount river ( Alpinia officinarum Hance ) warms the stomach to increase the digestive function of the stomach and promotes the secretion of gastric juice, so it is effective in chronic gastritis, anorexia, vomiting, abdominal pain, food, stomach and soreness. Drug decoction inhibits the development of hemolytic streptococci, diphtheria and pneumococci. Α-cadinene, cineol, and cinnamic acid, which are the major components of high-quality steel, are natural organic compounds with phenolic structure. They are effective for removing bacteria, viruses, molds, and itching. It is known to be excellent for treating purulent skin and acne. In addition, the fragrant aroma and cool feeling of essential oils contained in high quantity river purify the skin, and it is said to have the effect of soothing burns and sunburned skin.

Goyang River is a perennial herb that is about 1 meter high belonging to Gingeraceae and uses dried root stems in autumn. In motion therapy, it is said to be effective in abdominal pain, vomiting and diarrhea, and in response to abdominal pain and diarrhea caused by eating cold foods, beverages, and fruit, it is said to be effective in weakness, diarrhea and dysentery due to renal weakness and coldness. Essential oils contain 0.5-1.5%, and the main ingredients are cineol, methylcinnamate, eugenol, pinene and cardinene. In addition, the flavonoids contain galangin, camperide, kaempferol, quercetin, isoramnetine, galanzine-3-methyl ether, and the like and galangol as a spice component. The main pharmacological action is known to include gastric secretion promoting action, analgesic action, antioxidant deficiency action, antithrombotic action, anticoagulant action, peripheral circulation improvement effect (components and use of herbal medicine, wolwoljeol).

In oriental medicine, high-volume steel is known to be effective in abdominal pain, vomiting and diarrhea caused by nausea, renal weakness, weakness due to coldness, diarrhea and dysentery. It has been reported that pharmacological action inhibits the development of hemolytic streptococci, diphtheria bacteria and pneumococci. The shape is bent cylindrical or conical with several side roots. The outer side has reddish brown gray white spinal cord every 5 ~ 10 mm, and there are longitudinal wrinkles between the nodes. There is a round mark on the lower part where the roots are attached. The quality is hard and not easily bent. Folded sides are yellowish red to reddish brown with clear skin and neck and fibrous. By other names, Yanggang, Haiyang River, Soyang River, Mangang, Lianggang, Xinjiang, Soxin River, Goshingang (膏 身 薑) and the like. However, nothing has been disclosed or disclosed in the above literature with regard to high dose kidney cancer related diseases.

Thus, the present inventors completed the present invention by confirming that the high-volume extract can effectively kill kidney cancer cells during the herbal medicine study for the high-volume steel.

It is an object of the present invention to provide a composition for the treatment of kidney cancer and a cosmetic composition containing a high amount of the extract as an active ingredient.

In order to achieve the above object, the present invention provides a composition for the prevention and treatment of kidney cancer containing Alpiniae Officinari Rhizoma ethanol extract as an active ingredient.

In another aspect, the present invention provides a cosmetic composition for preventing kidney cancer, containing a high- strength ( Alpiniae Officinari Rhizoma ) ethanol extract containing a cosmetically acceptable cosmetic supplement additive as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for the prevention and treatment of kidney cancer containing Alpiniae Officinari Rhizoma ethanol extract as an active ingredient. The composition of the present invention comprises a high-volume extract as an active ingredient, and may further include a pharmaceutically acceptable carrier or diluent.

In the composition for preventing and treating kidney cancer of the present invention, the ethanol extract is preferably extracted at 50 ° C. for 24 hours, wherein the ethanol extract is more preferably dried and concentrated at 45 ° C. under reduced pressure, and the ethanol Is most preferably 95%.

In addition, in the composition for preventing and treating kidney cancer of the present invention, the kidney cancer is preferably renal cell cancer.

The high amount river extract of the present invention is possible at any part of the high amount steel, but is preferably extracted from the root. The extraction solution may be obtained by extraction with water or an organic solvent, and examples of the organic solvent may include lower alcohols, acetone, chloroform, methylene chloride, ether, ethyl acetate, and hexane. Lower alcohols include methanol, ethanol, propanol and butanol, with ethanol being most preferred.

Specifically, 1 to 5 times, preferably 3 times, 95% ethanol is added to the high volume steel building or powder, and 10 to 100 hours, preferably 15 at a temperature of 20 to 100 ° C, preferably 40 to 60 ° C. To 40 hours, more preferably, 24 hours after the extraction can be filtered to produce a high-volume ethanol extract. Preferably, the filtrate obtained by filtering the extract may be concentrated under reduced pressure. In the above extraction methods, the extraction process may be repeated two or more times as necessary, and the extract obtained after filtration may be lyophilized or dried under reduced pressure to obtain a powder form.

The "pharmaceutically acceptable carrier" is a pharmaceutically acceptable substance such as a liquid or solid filler, diluent, excipient or solvent which serves to transport the active ingredient from one organ or part of the body to another organ or part of the body. , Composition or vehicle.

The composition for treating kidney cancer of the present invention may be prepared as a medicament by adding one or more pharmaceutically acceptable carriers together with the active ingredient. The carrier may include, but is not limited to, saline, buffered saline, water, glycerol and ethanol, and any suitable agent known in the art (Remingtons's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA) may be used. .

Formulations for pharmaceutical preparation of high-volume steel extract of the present invention can be administered orally during clinical administration and can be used in the form of general pharmaceutical formulations, and when formulated, commonly used fillers, extenders, binders, wetting agents, disintegrants And diluents such as surfactants or excipients. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and liquid preparations for oral use include suspensions, solvents, emulsions, and syrups. In addition to liquids and paraffins, various excipients may be included, such as wetting sweeteners, fragrances, preservatives and the like.

The composition of the present invention may be administered in various parenteral formulations during actual clinical administration, and solid preparations include tablets, pills, powders, granules, capsules, and the like. In addition to water, liquid, and paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. Specifically, preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like. In addition, calcium or vitamin D ₃ may be added to enhance the efficacy of the treatment. Such compositions may be presented in unit-dose (single) or multi-dose (several) containers, such as sealed ampoules and vials, and immediately before use, sterile liquid carriers such as injectable water. Can be stored under freeze-drying conditions requiring only the addition of. Immediate injection solutions and suspensions can be prepared from sterile powders, granules and tablets.

The formulations of the present invention can be applied differently depending on the age, sex, condition of the subject, the absorption of the active ingredient in the body, the inactivation rate and excretion rate, the drug used in combination. The invention also includes formulations of dosage units. The formulations are present in individual dosage forms, such as tablets, coated tablets, capsules, pills, suppositories, and ampoules, wherein the amount of active compound in the drug corresponds to the fraction or multiple of the individual dosage. Dosage units may contain, for example, one, two, three or four times the individual dosage, or 1/2, 1/3 or 1/4 times. The individual dosages preferably contain an amount in which the active compound is administered at one time, which usually corresponds to all, 1/2, 1/3 or 1/4 times the daily dosage.

As used herein, the term "extract" refers to an active ingredient isolated from natural products. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or any form formulated using the same.

In a specific embodiment of the present invention, high-strength ethanol extract killed 98.1% of ACHN kidney cancer cells at 100 μg / ml, and the EC ₅₀ (half maximal effective concentration) was 29.7 μg / ml. The above results demonstrate that the high-yield river extract of the present invention has excellent killing activity of ACHN kidney cancer cells and further has therapeutic and prophylactic activity for kidney cancer.

As used herein, the term "prevention" means any action that inhibits or delays the development of kidney cancer by administration of the composition.

As used herein, the term "treatment" means any action that improves or advantageously changes the symptoms of kidney cancer by administration of the composition.

High amount steel extract in the present invention can be used to extract using water, an organic solvent, or a mixed solvent thereof. Preferably it is extracted using an organic solvent, in particular ethanol. The extracted solution can be used directly or can be concentrated and / or dried. When extracted with an organic solvent, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof may be used and extracted by room temperature or warming under conditions where the active ingredient of the herbal medicine is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and loss of the active ingredient of the drug may vary, so select an appropriate organic solvent. The extraction method is not particularly limited, and examples thereof include cold needle extraction, ultrasonic extraction, reflux cooling extraction, and the like. Filtration is a process of removing the suspended solid particles from the extract, it may be used to filter the particles using cotton, nylon or the like, or may be used, such as ultrafiltration, cryofiltration, centrifugal separation, but is not limited thereto.

Concentration of the extract may be used, such as concentrated under reduced pressure, reverse osmosis concentration. The drying step after concentration includes freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying, and the like. If desired, a process of grinding the final dried extract may be added.

In addition, the extract can perform an additional fractionation process. Preferably, the extract is suspended in distilled water to obtain a nonpolar solvent soluble layer by extraction and separation with a nonpolar organic solvent such as hexane, ether, dichloromethane, chloroform, ethyl acetate, or a mixed solvent thereof. It can be used by concentrating and / or drying it.

In the present invention, the term "pharmaceutically acceptable salts" means salts derived from pharmacologically or physiologically acceptable inorganic acids, organic acids and bases. Examples of suitable acids include hydrochloric acid, bromic acid, sulfuric acid, nitric acid, perchloric acid, fumaric acid, maleic acid, phosphoric acid, glycolic acid, lactic acid, salicylic acid, succinic acid, toluene-p-sulfonic acid, tartaric acid, acetic acid, citric acid, methanesulfonic acid, formic acid , Benzoic acid, malonic acid, naphthalene-2-sulfonic acid, benzenesulfonic acid, and the like. Salts derived from suitable bases may include alkali metals such as sodium, alkaline earth metals such as magnesium, ammonium and the like.

The pharmaceutical composition for preventing and treating kidney cancer diseases of the present invention comprises 0.1 to 50% by weight of the extract or compound based on the total weight of the composition. In addition, the composition does not increase the efficacy, but may include additional ingredients that are commonly used in the pharmaceutical composition to improve the smell, taste, time and the like. In addition, the composition adds inorganic and organic additives such as vitamins B1, B2, B6, C, E, niacin, carnitine, betaine, folate pantothenic acid, biotin, zinc, iron, calcium, chromium, magnesium, and mixtures thereof. It can be included as. In addition, the composition may include a substance having a therapeutic activity against kidney cancer used alone or used in the past.

As used herein, the term "patient" refers to humans, horses, sheep, pigs, goats, and camels having a disease caused by kidney cancer and its direct and indirect causes, and whose symptoms may be improved by administering the composition of the present invention. Means animals such as nutrition, dogs. By administering to the patient a composition comprising a high dose of the extract of the present invention, it is possible to effectively prevent and treat the above-mentioned kidney cancer. The composition of the present invention can be administered in parallel with existing kidney cancer treatments.

As used herein, the term "administration" means introducing a predetermined substance into a patient by any suitable method, and the route of administration of the composition of the present invention is oral or parenteral via any general route as long as the target tissue can be reached. May be administered. In addition, the composition may be administered by any device in which the active agent may migrate to the target cell.

The composition of the present invention is administered in a pharmaceutically effective amount. As used herein, the term “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dose level refers to a patient's sexually transmitted disease, age, severity, and drug activity. , Drug sensitivity, time of administration, route of administration and rate of release, duration of treatment, factors including concurrently used drugs, and other factors well known in the medical arts. The compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. It may be single or multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art. The method of administering the composition comprising the extract or compound prepared according to the preparation method of the present invention is preferably oral administration or intravenous administration. In general, when the effective dose is oral administration, it is usually 1 to 1 time per adult. 500 mg / kg is preferred, and in the case of intravenous administration, 1 to 100 mg / kg is preferred, and may be administered 2-3 times a day. Dosage levels for a particular patient may vary depending on sex, age, health condition, diet, time of administration, method of administration, drug mixture, the condition of the patient, and the extent of the onset of neurological disease.

In another aspect, the present invention provides a cosmetic composition for preventing kidney cancer, containing a high- strength ( Alpiniae Officinari Rhizoma ) ethanol extract containing a cosmetically acceptable cosmetic supplement additive as an active ingredient.

The cosmetic composition of the present invention preferably contains 0.01 to 10% by weight, and more preferably 0.1 to 1% by weight, based on the total weight of the ethanol extract. The cosmetic composition of the present invention is not particularly limited in the formulation, for example, it may have a flexible cosmetic water, nourishing cosmetics, massage cream, nutrition cream, pack, gel or skin adhesive cosmetic formulation, and also lotion, It may be a transdermal dosage form such as an ointment, gel, cream, patch or spray.

In the present invention, it was confirmed that the ACHN kidney cancer cells die as a result of injecting the high amount of the extract of the present invention into ACHN kidney cancer cells (see FIG. 1 ). Therefore, it can be seen that the high-yield extract has a therapeutic effect on kidney cancer. Thus, in the present invention, the present invention was completed by preparing a cosmetic composition for preventing kidney cancer containing a high amount of the extract as an active ingredient (see Preparation Example 2).

As described above, the high amount of the extract of the present invention inhibits the growth of kidney cancer cells and induces apoptosis. Therefore, the composition for treating renal cancer according to the present invention will be very effective in the treatment of renal cancer patients.

1 is a result of Alamar Blue analysis to determine the effect of the introduction of high-volume steel on the growth of kidney cancer cells in human kidney cancer ACHN cells, wherein the X-axis is the concentration of high-volume extract, Y-axis of the surviving human kidney cancer cells Survival rate.

Hereinafter, the present invention will be described in more detail based on the following examples. It should be noted, however, that the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The present invention is not limited to the following examples. Will be apparent to those skilled in the art to which the present invention pertains.

Example 1 Preparation of High-Range Steel Extract

3 kg of high quantity steel (made in China) purchased from Seoul medicinal herb was dried and ground for 5 days at the shade and room temperature. The ground high volume steel was immersed in 30 L of 95% ethanol and extracted at 50 ° C. for 24 hours. It was filtered through filter paper, dried and concentrated under reduced pressure at 45 ° C. to obtain 492 g of the total extract, which was stored at −20 ° C.

Example 2 Effect of High-Range River Extract on the Growth of Kidney Cancer Cells

In order to determine the effect of the high-volume extract extracted in Example 1 on the growth of kidney cancer cells, ACHN cells, which are human kidney cancer cells, were treated with high-volume ethanol extract for 48 hours and subjected to Alamar Blue analysis. The Alamar Blue assay is a modified form of the MTT assay, in which a specific enzyme degrades a living cell and then measures the fluorescence intensity of the product as the compound breaks down to determine the relative number of living cells after treatment. I am an experimental method. It will be described in more detail below.

<2-1> Preparation and Treatment of Human Kidney Cancer Cell Line

ACHN cells, a kidney cancer cell line used in the present invention, were distributed from Korean Cell Line Bank (KCLB) and used for experiments. Specifically, ACHN kidney cancer cells (Minimum essential medium) containing 10% FBS (fetal bovine serum, fetal bovine serum) (Welgene) and 25 mM HEPES (4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid) Subcultured in medium.

<2-2> Measurement of Cell Growth Inhibition of ACHN Cells

The high-volume extract extracted in Example 1 was confirmed to inhibit the growth of ACHN cells, which are kidney cancer cells. Specifically, after the injection of 6.0 × 10 ³ ACHN kidney cancer cells per well in a 96 well plate and incubated for 24 hours, the high-quality steel dissolved in DMSO (dimethyl sulfoxide) When the ethanol extract was treated at concentrations of 0 to 100 μg / ml (specifically, concentrations of 0, 3.125, 6.25, 12.5, 25, 50, and 100 μg / ml, respectively) for 48 hours, the degree of inhibition of cell growth was confirmed. (Table 1). After treatment with each concentration of extract, 20 μl of Alamar Blue reagent was added to 0.2 ml of cell culture filled in each well in a 96-well plate, and the plates were incubated for 2 hours in an incubator. The plate was slowly shaken to evenly react the cells in each well, and the intensity of fluorescence was measured at 590 nm with a Fluorescence Microplate Reader (Molecular Devices Corp.) while irradiating irradiated light at a wavelength of 544 nm. The survival rate of is shown in FIG.

High river concentration Kidney Cancer Cell Survival (Average) Standard Deviation 0 μg / ml 1,000 0.000 3.125 μg / ml 1.002 0.023 6.25 μg / ml 0.996 0.008 12.5 μg / ml 0.942 0.017 25 μg / ml 0.698 0.032 50 [mu] g / ml 0.080 0.002 100 μg / ml 0.019 0.001

As a result, as shown in Table 1 and Figure 1, the higher the treatment concentration of the high amount of steel, the growth of renal cancer cells was reduced, from which it can be seen that the high amount of steel has a renal cancer treatment effect. That is, renal cancer cells were killed at 0.40% at 6.25 μg / ml, 5.8% at 12.5 μg / ml, 30.2% at 25 μg / ml, 92.0% at 50 μg / ml, and 98.1% at 100 μg / ml. In addition, an EC ₅₀ (half maximal effective concentration) was measured at 29.7 μg / ml. In Table 1, the relative cell numbers of kidney cancer cells after 48 hours according to each high-strength treatment concentration based on the number of survival rates of kidney cancer cells of the control group not treated with high-volume steel were described. As such, the high-yield river extract of the present invention has excellent ACHN cell killing activity and further demonstrates the therapeutic and prophylactic activity of kidney cancer.

Example 3 Acute Toxicity Test by High-Range Steel Extract

The high amount of steel used in the present invention was widely used as a medicinal herb, and thus it was judged that there would be no problem in stability.

Acute toxicity test was performed using 6-week-old SPF SD rats. Two animals per group were suspended orally administered at a dose of 5 g / kg in a high-strength extract of Example 1 of the present invention, respectively, in 0.5% methylcellulose solution. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed, and hematological and hematological examinations were performed.

As a result, there were no clinical symptoms or deaths in all animals treated with the test substance, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc. As a result, all the high-yield extracts did not show toxicological changes in rats up to 5 g / kg and the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance of 5 g / kg or more.

Preparation Example 1 Preparation of Renal Cancer Therapeutic Agents Containing High-Range River Extract as Active Ingredient

The present inventors have confirmed that the renal cancer treatment effect of the high-volume river extract is excellent through the above example, and prepared a renal cancer therapeutic agent containing the extract as an active ingredient as follows. In addition, the preparation of the following therapeutic agents can be used for the application of not only therapeutic agents but also cosmetic compositions.

<1-1> Soft gelatin capsules (wt%) containing high amount of extract

Goyang River Extract 20%

Vitamin C 4.5%

Vitamin D ₃ 0.001%

Manganese Sulfate 0.1%

10% wax

Palm oil 25%

Safflower Seed Oil 30.399%

 <1-2> Preparation of Intravenous Formulations Containing High-Quality Steel Extracts (wt%)

Goyang River Extract 0.2%

Mannitol 0.3%

Saline 9.5%

<1-3> tablets (wt%) containing high-volume steel extract

Goyang River Extract 35%

Vitamin C 10%

Vitamin D ₃ 0.001%

Manganese Sulfate 0.1%

Crystalline cellulose 25.0%

Lactose 17.999%

Magnesium Stearate 2%

Preparation Example 2 Preparation of Cosmetic Composition Containing High-Range Steel Extract as Active Ingredient

The present inventors have confirmed that the high-volume river extract is excellent in the renal cancer treatment activity through the above embodiment to prepare a cosmetic composition containing the same as an active ingredient as follows.

<2-1> Nutritional Cosmetic Water Containing High-Range River Extract (wt%)

Nutrients were prepared in a conventional manner according to the composition described below.

Glycerin 8.0%

Butylene Glycol 4.0%

Hyaluronic Acid Extract 5.0%

Beta Glucan 7.0%

Carbomer 0.1%

Goyang River Extract 0.05%

Capric and Capric Triglycerides 8.0%

Squalane 5.0%

Cetearyl Glucoside 1.5%

Sorbitan stearate 0.4%

Cetearyl Alcohol 1.0%

Triethanol Amine 0.1%

Purified water level

<2-2> Nutritional Cream Containing High-Range River Extract (wt%)

The nourishing cream was prepared by a conventional method according to the composition described below.

Glycerin 3.0%

Butylene Glycol 3.0%

Liquid paraffin 7.0%

Beta Glucan 7.0%

Carbomer 0.1%

Goyang River Extract 3.0%

Capric & Capric Triglycerides 3.0%

Squalane 5.0%

Cetearyl Glucoside 1.5%

Sorbitan stearate 0.4%

Polysorbate 60 1.2%

Triethanol Amine 0.1%

Purified water level

<2-3> massage cream containing a high amount of river extract (% by weight)

Massage creams were prepared in a conventional manner according to the composition described below.

Glycerin 8.0%

Butylene Glycol 4.0%

Liquid paraffin 45.0%

Beta Glucan 7.0%

Carbomer 0.1%

Goyang River Extract 1.0%

Capric & Capric Triglycerides 3.0%

Beeswax 4.0%

Cetearyl Glucoside 1.5%

Sesqui oleic acid sorbitan 0.9%

Vaseline 3.0%

Paraffin 1.5%

Purified water level

<2-4> Pack containing a high quantity river extract (wt%)

Packs were prepared by conventional methods according to the compositions described below.

Glycerin 4.0%

Polyvinyl Alcohol 15.0%

Hyaluronic Acid Extract 5.0%

Beta Glucan 7.0%

Allantoin 0.1%

Goyang River Extract 0.5%

Nonyl Phenyl Ether 0.4%

Polysorbate 60 1.2%

Ethanol 6.0%

Purified water level

<2-5> Ointment for external application of skin containing high quantity steel extract (% by weight)

Ointments were prepared in a conventional manner according to the compositions described below.

Glycerin 8.0%

Butylene Glycol 4.0%

Liquid paraffin 15.0%

Beta Glucan 7.0%

Carbomer 0.1%

Goyang River Extract 3.0%

Squalane 1.0%

Cetearyl Glucoside 1.5%

Sorbitan stearate 0.4%

Cetearyl Alcohol 1.0%

Beeswax 4.0%

Purified water level

On the other hand, the specific scope of the present invention is defined by the claims rather than the embodiments described above, all changes and modifications derived from the meaning and scope and equivalent concepts of the claims to the scope of the invention It should be interpreted as including.

Claims (7)

Alpiniae Officinari Rhizoma A composition for the prevention and treatment of kidney cancer, which contains ethanol extract as an active ingredient.
According to claim 1, wherein the ethanol extract is a composition for preventing and treating kidney cancer, characterized in that extracted for 24 hours at 50 ℃.
The composition for preventing and treating kidney cancer according to claim 1 or 2, wherein the ethanol extract is dried and concentrated at 45 ° C under reduced pressure.
According to claim 1 or claim 2, wherein the ethanol is a composition for the prevention and treatment of kidney cancer, characterized in that 95%.
The method of claim 1, wherein the composition is a composition for preventing and treating kidney cancer, characterized in that it comprises a pharmaceutically acceptable carrier or diluent.
The composition for preventing and treating kidney cancer according to claim 1, wherein the kidney cancer is renal cell cancer.
A cosmetic composition for preventing kidney cancer, comprising an ethanol extract of Alpiniae Officinari Rhizoma containing an cosmetically acceptable cosmetic supplement as an active ingredient.

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