KR20120109934A - Bacillus subtilis kkg-1 and microbial agent and biopesticide containing the same - Google Patents
Bacillus subtilis kkg-1 and microbial agent and biopesticide containing the same Download PDFInfo
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Abstract
Description
본 발명은 고추역병 방제에 있어서 고추역병균 파이토프소라 캡사이시( Phytophthora capsici)에 항균 활성을 갖으면서 작물 생육을 촉진하는 신규한 균주 바실러스 섭틸러스(Bacillus subtilis) KKG-1과 이를 유효성분으로 함유하는 미생물제제 및 미생물농약에 관한 것으로, 더욱 상세하게는 길항성과 작물 생육 촉진을 동시에 갖는 균주를 분리동정하고 이 균주를 유효성분으로 함유하는 미생물제제 및 미생물농약에 관한 것이다.The present invention is a novel strain Bacillus subtilis KKG-1 and active ingredient which promotes crop growth while having antibacterial activity against phytophthora capsici The present invention relates to a microbial agent and a microbial pesticide containing the same, and more particularly, to a microorganism and a microbial pesticide containing and identifying a strain having both antagonism and crop growth promotion at the same time.
미생물 제제 및 농약의 개발에 있어 필수적인 요소는, 길항성이 뛰어난 유용 미생물을 선발하거나 변형하여 만들어내고, 선발된 미생물 또는 미생물이 분비하는 약제를 대량 생산하여 이들을 농작물에 효율적으로 전달하여 사용하는데 있다. 최근 미생물을 이용한 미생물농약의 연구개발이 활발히 이루어지고 있다.An essential element in the development of microbial preparations and pesticides is to select or modify useful microorganisms with excellent antagonism, to mass produce the selected microorganisms or drugs secreted by the microorganisms, and to efficiently deliver them to the crops for use. Recently, research and development of microbial pesticides using microorganisms has been actively conducted.
이와 관련하여 종래기술로는 한국 특허출원 1990-0017551호(신규의 바실러스 서브틸리스 아종 및 이로부터 생산되는 항진균 물질 KFR-001 복합체의 용도)에 항진균 올리고 펩티드계 물질을 생산하는 바실러스 서브틸리스 아종 크릭티엔시스 (Bacilus subtilis subsp. Krictiensis) 에 관한 것이 있으나, 항진균 활성 스펙트In this regard, the prior art is Bacillus subtilis subspecies producing antifungal oligopeptide-based material in Korean Patent Application No. 1990-0017551 (new Bacillus subtilis subspecies and the use of the antifungal substance KFR-001 complex produced therefrom). Related to Bacilus subtilis subsp.Krictiensis, but antifungal activity spec
럼이 좁은 문제점이 있었다.Rum had a narrow problem.
또한 한국 특허출원 1998-0062437호(병원성 진균에 대한 항진균 활성을 갖는 미생물, 이를 함유하는 항진균성 미생물 농약제 및 그의 제조방법)에는, 바실러스 섭틸리스 변종 아밀로리쿠파시엔스(Bacilius subtilis var. amyloliquefaciens), 슈도모나스 아에루기노사(Pseudomonas aeruginosa)등 항진균 활성을 나타내는 미생물과 이를 이용한 바이오 매트릭스에 관한 것이 기재되어 있으나, 바이오 매트릭스의 구성이 복잡하고 항진균 활성 스펙트럼이 좁은 문제점이 있었다.In addition, Korean Patent Application No. 1998-0062437 (microorganisms having antifungal activity against pathogenic fungi, antifungal microbial pesticides containing the same and preparation methods thereof), Bacillius subtilis var. Amyloliquefaciens (Bacilius subtilis var. Amyloliquefaciens) ), Pseudomonas aeruginosa (Pseudomonas aeruginosa) and the like showing a microorganism that exhibits antifungal activity and a biomatrix using the same, but the composition of the bio-matrix and a narrow antifungal activity spectrum has a problem.
한편, 본 발명의 대상이 되는 고추는 열대와 아열대 지방의 중요한 작물로 우리나라뿐만 아니라 전 세계적으로 소비량이 많은 작물이며, 농가와 소비자들에게 영양학적 및 경제적인 가치가 매우 높다. 최근 고추 연작재배로 인하여 토양의 물리화학적 성질을 악화시키고, 독성 화합물의 축적으로 식물 병원균의 내성을 증가시켜On the other hand, the red pepper which is the object of the present invention is an important crop of tropical and subtropical regions, a crop that is consumed not only in Korea but also worldwide, and has high nutritional and economic value to farmers and consumers. Recent pepper cultivation worsens the physicochemical properties of soil and increases the resistance of plant pathogens by accumulating toxic compounds.
역병 방제의 어려움을 가지고 있다.Has difficulty in controlling plague.
고추에 심각한 병을 일으키는 것중 하나인 역병은 난균강인 파이토프소라 캡사이시( Phytophthora capsici)The late blight, which causes serious illness in peppers, is Phytophthora capsici, a fungal steel
에 의한 것으로, 병징은 뿌리와 crown 부패, 고추의 잎, 줄기, 열매의 마름병을 일으킨다(Mao등, 1998). 상기 파이토프소라에 의한 병이 고추 생산량을 감소시키는 가장 중요한 원인으로 보고되어 왔지만, 효과적인 방제Symptoms cause root and crown rot, blight of pepper leaves, stems and fruits (Mao et al., 1998). Although the disease caused by the phytopsora has been reported as the most important cause of reducing pepper production, effective control
법은 없는 실정이다. 그 이유는 파이토프소라 캡사이시( Phytophthora capsici)의 난포자가 내건성이고, 낮은 온도와 다른 극심한 악조건에서도 저항성이 강해 수년동안 토양내에서 기주식물 없이도 생존할 수 있기 때문이다.There is no law. The reason is that the follicles of Phytophthora capsici are dry tolerant and resistant to low temperatures and other extreme adverse conditions, allowing them to survive without host plants in the soil for many years.
방제법으로는 연작의 회피와 이랑을 높게 하여 배수를 좋게 하면 병의 관리에 도움이 되지만 완벽한 방제법이라 할 수 없다. 또한, 메틸 브로마이드와 같은 살균제를 사용하여 좋은 결과를 얻을 수 있으나 식물 독소와 잔여 농약은 환경오염과 인체건강을 위협하는 주요한 문제로서 대두되고 있다. 더욱이 토양내 잔여농약은 유용As a control method, avoiding the series and raising the mockup to improve drainage helps manage the disease, but it is not a perfect control method. In addition, good results can be obtained by using fungicides such as methyl bromide, but plant toxins and residual pesticides are emerging as major problems that threaten environmental pollution and human health. Moreover, residual pesticides in soils are useful
미생물의 밀도와 식물생장에 영향을 주어 작물 생산량의 감소를 초래하고 있다.It affects the density of microorganisms and plant growth, leading to a decrease in crop yield.
위와 같은 이유로 고추역병에 길항하며 작물의 생육에 도움을 줄 수 있는 다기능성 균주에 기초한 농약제를 제공할 필요가 제기되고 있다. 특히 최근 친환경 농산물의 수요가 급증하여 화학농약의 사용을 줄이고 친환경적 제품들의 사용이 증가하는 추세에 있으나 대부분 외국에서 제재화된 수입품들이며 국내 미생물로 방제하는 경우는 드물기에 신규한 미생물을 제공할 필요가 있다.For these reasons, there is a need to provide pesticides based on multifunctional strains that can antagonize pepper disease and help crop growth. In particular, the demand for eco-friendly agricultural products has soared recently, reducing the use of chemical pesticides and increasing the use of eco-friendly products. However, most of them are imported from foreign countries and rarely need to provide new microorganisms. have.
본 발명은 상기한 문제점을 해결하기 위하여 안출된 것으로서, 고추역병의 병원균인 파이토프소라 캡시사이시에 대해 항균활성과 함께 작물 생육을 촉진하는 신규의 균주를 분리동정하고, 이 균주를 함유하는 미생물제제 및 농약을 제공함에 그 목적이 있다.The present invention has been made to solve the above problems, isolates and identifies new strains that promote crop growth with antimicrobial activity against the phytoplasma capsicici, a pathogen of red pepper disease, microorganisms containing the strain The purpose is to provide formulations and pesticides.
본 발명의 상기 목적은 고추역병의 병원균인 파이토프소라 캡시사이의 생육을 억제하는 작용에 더하여 길항균에서 분비하는 물질(ACC deaminase)이 식물의 스트레스를 줄여줌으로써 생육을 촉진시키는 균주 바실러스 섭틸러스 KKG-1을 분리동정하고, 분리동정한 균주 바실러스 섭틸러스 KKG-1의 파이토프소라 캡시사에The above object of the present invention, in addition to the action of inhibiting the growth of phytophthora capsisai, a causative agent of pepper disease, the bacterium Bacillus subtilis KKG, which promotes growth by reducing the stress of the plant (ACC deaminase) secreted by antagonists -1 was isolated and identified in the phytophora capsaisa of the strain Bacillus subtilis KKG-1.
대한 항균 활성과 길항균에서 분비하는 물질이 식물의 스트레스를 줄여 생육을 촉진시키는 활성을 확인한다.Antimicrobial activity against antagonists and substances secreted by the plant to reduce the stress to promote the activity to confirm the growth.
음, 바실러스 섭틸러스 KKG-1을 함유하는 미생물제제 및 농약을 제조하여 고추 역병 발생 억제 효과를 확인함으로써 완성하였다.Well, microbial preparations and pesticides containing Bacillus subtilis KKG-1 were prepared to confirm the inhibitory effect of pepper blight.
본 발명의 일견지에 의하면,고추역병균 파이토프소라 캡사이시에 대해 항균활성을 가지는 바실러스 섭틸러스 KKG-1 균주를 제공한다. According to an aspect of the present invention, it provides a Bacillus subtilis KKG-1 strain having an antimicrobial activity against the pepper disease bacteria phytopsora capsaish.
본 발명의 제2 견지에 의하면,상기 제1 견지에 의한 균주 또는 그 배양액을 유효성분으로 함유하는 고추역병 발생억제용 미생물제제를 제공한다.According to the second aspect of the present invention, there is provided a microbial agent for inhibiting the development of red pepper disease, which comprises the strain according to the first aspect or its culture solution as an active ingredient.
본 발명의 제3 견지에 의하면,According to the third aspect of the present invention,
상기 제1 견지에 의한 균주 또는 그 배양액을 유효성분으로 함유하는 고추역병 발생억제용 미생물농약을 제공한다.Provided is a microorganism pesticide for inhibiting the development of pepper blight, comprising the strain according to the first aspect or a culture medium thereof as an active ingredient.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
이상에서 설명한 바와 같이, 본 발명 바실러스 섭틸러스 KKG-1은 항진균 활성에 더하여 작물 생육을 촉진할As described above, the Bacillus subtilis KKG-1 of the present invention may promote crop growth in addition to antifungal activity.
뿐 아니라 유기물 분해능, 농약내성 및 농약분해능을 지니고 있어 환경보전 및 정화작용이 있는 다기능성 농In addition, it is a multifunctional agricultural plant with environmental preservation and purification, as it has organic substance resolution, pesticide tolerance and pesticide resolution.
약제로 유용하게 사용될 수 있다.It can be usefully used as a medicament.
도 1은 본 발명의 일 실시예에 의한 다기능성 효과중 식물병원균의 예방 효과를 도시한 도면으로 구체적으로
(a)는 식물병원균인 Phytophthoracapsici에 길항하는 사진(A:길항균주, B:식물병원균)을, (b)는 처리구의 유묘기 때 길항작용사진(C:처리, D:무처리)을, (c)는 전처리한 미생물이 잎에서 식물병원균감염을 예방한 사진(C: 무처리, D: 처리)을 의미한다.1 is a view showing the preventive effect of phytopathogens among the multifunctional effect according to an embodiment of the present invention specifically
(a) shows a picture (A: antagonist strain, B: plant pathogen) antagonistic to Phytophthoracapsici, phytopathogen, (b) shows a picture of antagonism (C: treatment, D: no treatment) during the seedling period of the treatment group, ( c) refers to the photograph (C: no treatment, D: treatment) in which the pretreated microorganisms prevented phytopathogen infection from the leaves.
이상에서 설명한 바와 같이, 본 발명 바실러스 섭틸러스 KKG-1은 항진균 활성에 더하여 작물 생육을 촉진할 뿐 아니라 유기물 분해능, 농약내성 및 농약분해능을 지니고 있어 환경보전 및 정화작용이 있는 다기능성 농약제로 유용하게 사용될 수 있다.As described above, the Bacillus subtilis KKG-1 of the present invention not only promotes crop growth in addition to antifungal activity, but also has an organic decomposition ability, pesticide resistance and pesticide decomposition ability, which is useful as a multifunctional pesticide with environmental conservation and purification. Can be used.
부호를 설명하겠습니다Let me explain the sign
Claims (1)
발현하여 작물의 생육을 촉진하는 바실러스 섭틸러스(Bacillus subtilis) KKG-1 (KCTC11124BP) 균주.1-aminocyclopropane-1-carboxylate deaminase (ACCD) gene with antibacterial activity against Phytophthora capsici
Bacillus subtilis KKG-1 (KCTC11124BP) strain that expresses and promotes the growth of crops.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112592840A (en) * | 2018-04-02 | 2021-04-02 | 叶志坚 | Compound microbial preparation and application thereof in prevention and treatment of phytophthora |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112592840A (en) * | 2018-04-02 | 2021-04-02 | 叶志坚 | Compound microbial preparation and application thereof in prevention and treatment of phytophthora |
| CN112592840B (en) * | 2018-04-02 | 2022-08-19 | 山东土木启生物科技有限公司 | Compound microbial preparation and application thereof in prevention and treatment of phytophthora |
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