KR20120109141A - Composition for treatment of lung cancer and functional food comprising extract of gleditsiae semen - Google Patents

Abstract

PURPOSE: A composition containing Gleditsiae Semen ethanol extract and a functional food are provided to suppress lung cancer cell growth and to prevent and treat lung cancer. CONSTITUTION: A composition for preventing and treating lung cancer contains Gleditsiae Semen ethanol extract as an active ingredient. The ethanol extract is prepared by extracting Gleditsiae Semen at 50 Deg. C. for 24 hours and drying and concentrating the extracting at 45 Deg. C. The composition contains pharmaceutically acceptable carriers or diluents. [Reference numerals] (AA) Gleditsiae Semen

Description

Composition for Treatment of Lung Cancer and Functional Food Comprising Extract of Gleditsiae Semen}

The present invention relates to a novel use of extracts which are flakes. Specifically, the present invention relates to a therapeutic composition and a functional food containing the extract as an active ingredient, which is a fragment showing excellent preventive or therapeutic efficacy against lung cancer.

Among the major diseases of modern people, researches on the treatment and diagnosis of cancer are being conducted mainly on lung cancer, liver cancer, and stomach cancer, which have high incidence. Lung cancer is a malignant tumor in the lung, and it can be divided into primary lung cancer, which is the first cancer cell in bronchial tissue, bronchiole, alveoli, etc., and metastatic lung cancer in which cancer cells are formed in other organs and move to the lungs through blood vessels or lymphatic vessels. have.

According to the Korea Central Cancer Registry released in 2009, 161,920 cases of cancer occurred in Korea in 2007. Among them, lung cancer was 17,846 cases annually, which ranked 4th with 11.0% of all cancer cases. . The incidence rate per 100,000 population is 36.3. The sex ratio of men and women was 3.51: 1, which was higher in men. The incidence was 12,841 cases per year for men, ranking second among men's cancers, and women's number 5,005 per year, ranking fifth among women's cancers. By age group, 70s accounted for 34.1%, followed by 31.3% in the 60s, and 14.8% in the 50s. (A data released on December 21, 2009, by the Ministry of Health, Welfare and Family Affairs).

Lung cancer is classified microscopically into non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC) according to the size and shape of the cancer cells. The differentiation between non-small cell lung cancer and small cell lung cancer is due to the different clinical course and treatment. Non-small cell lung cancer, which accounts for 80% to 85% of the total lung cancer, can be treated by surgical treatment when diagnosed early. Due to the nature of difficult lung cancer, it is often found late and difficult to treat. Non-small cell lung cancer consists of three subtypes: 40% adenocarcinoma, 40% squamous cell carcinoma and 20% large cell carcinoma. TMN staging system is widely accepted for the management of lung cancer. Primary tumors are divided into four T categories (T1-T4) according to tumor size, site and local involvement. Lymph node spread is delivered into the bronchus / pulmonary in the lung (N1), medial spread on the same side as the primary tumor (N2) and mediastinal spread across the primary lung tumor. Or supraclavilcular involvement (N3). There is no distant or metastatic spread (M0 or M1). In general, lung cancer that has not metastasized is treated by surgical removal. However, recurrence rates after lung cancer removal surgery are high, ranging from 20 to 50% (Cancer: Principles & Practice of Oncology, 56th. Ed. In: Devita DV, Hellman S, Rosenberg SA, eds. Philadelphia, PA: Lippincott Williams & Wilkins, 2001 ).

In particular, non-small cell lung cancer has a high recurrence rate after treatment, and compared with a high incidence rate, resistance to conventional chemotherapy with an anticancer agent is easily generated, making it difficult to treat effectively. In the case of the non-small cell lung cancer, the 10-year survival rate is very low, less than 10% despite the recent development of cancer therapy. Therefore, it is very important to develop a treatment for such non-small cell lung cancer.

Gleditsiae Semen is the seed of the juniper tree ( Gledisia japonica Miquel var.koraiensis Nakai). It is a herbal medicine used in various folk remedies such as controlling wind and improving bowel function. Thorn is called a sculptor, and it has the effect of sowing, drainage, etc., used in various boils. Overwintering is also possible in central Korea. Gleditsia sinensis Lam. Is a herb used in various folk remedies such as improving intestinal function in East Asia. Thorn is called a sculptor, and it has the effect of sowing, drainage, etc., used in various boils. Overwintering is also possible in central Korea.

However, there is no known sculptor's effect on inhibiting lung cancer in any literature.

Accordingly, the present inventors completed the present invention by confirming that the extract of the sculptor can effectively kill lung cancer cells while studying the herbal medicine for sculptor.

It is an object of the present invention to provide a composition for treating lung cancer and a functional food containing the extract as an active ingredient.

In order to achieve the above object, the present invention provides a composition for preventing and treating lung cancer containing Gleditsiae Semen ethanol extract as an active ingredient.

In addition, the present invention provides a functional food for preventing lung cancer containing Gleditsiae Semen ethanol extract containing a food supplement acceptable food supplement as an active ingredient.

 Hereinafter, the present invention will be described in detail.

The present invention provides a composition for the prevention and treatment of lung cancer containing Gleditsiae Semen ethanol extract as an active ingredient. The compositions of the present invention comprise extracts that are flakes as active ingredients and may further comprise pharmaceutically acceptable carriers or diluents.

In the composition for preventing and treating lung cancer of the present invention, the ethanol extract is preferably extracted for 24 hours at 50 ℃, wherein the ethanol extract is more preferably dried and concentrated under 45 ℃ reduced pressure conditions, the ethanol is Most preferred is 95%.

In addition, in the composition for preventing and treating lung cancer of the present invention, the lung cancer is preferably non-small cell lung cancer, wherein the non-small cell lung cancer is adenocarcinoma (adenocarcinoma), squamous cell carcinoma (squamous cell carcinoma) and large cell cancer ( More preferably caused by a carcinoma selected from the crowd consisting of large cell carcinoma.

As used herein, the term "lung cancer" refers to a malignant tumor occurring in the lung, and includes both small cell lung cancer and non-small cell lung cancer such as adenocarcinoma, squamous cell carcinoma, and large cell lung cancer.

The "pharmaceutically acceptable carrier" is a pharmaceutically acceptable substance such as a liquid or solid filler, diluent, excipient or solvent which serves to transport the active ingredient from one organ or part of the body to another organ or part of the body. , Composition or vehicle.

The composition for treating lung cancer of the present invention may be prepared as a medicament by adding one or more pharmaceutically acceptable carriers together with the active ingredient. The carrier may include, but is not limited to, saline, buffered saline, water, glycerol and ethanol, and any suitable agent known in the art (Remingtons's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA) may be used. .

Formulations for pharmaceutical formulation of the extract of the present invention can be administered orally during clinical administration and can be used in the form of general pharmaceutical formulations, when formulated, commonly used fillers, extenders, binders, wetting agents, disintegrants And diluents such as surfactants or excipients. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and liquid preparations for oral use include suspensions, solvents, emulsions, and syrups. In addition to liquids and paraffins, various excipients may be included, such as wetting sweeteners, fragrances, preservatives and the like.

In addition, the herbal medicine that may be added to the composition of the present invention may be any pharmaceutically acceptable herbal medicine, for example, Angelica tenuissimae Radix, Gastrodiae Rhizoma, Bapleuri Radix, Angelica ( Angelicae gigantis Radix, Persicae Semen, Cinnamomi Ramulus, Rhubarb (Rhei Rhizoma), Licorice (Glycyrrhizae Radix), Cnidii Rhizoma, Aurantii nobilis Pericarpium, Taxa (Alismatis Rhizoma) Coptidis Rhizoma, Scutellariae Radix, Hoelen, Peeoniae Radix, Atractylodis Rhizoma alba, Phellodendri Cortex, Gardeniae Fructus, Pinelliae Tuber, Ramulu Set (Uncaria) Uncus, Ponciri Fructus, Ginseng (Gingseng), Liriopis Tuber, Polygalae Radix, Acori graminei Rhizoma, Atractylodis Rhizoma alba, Chrysanthemi Flos, Windproof (Ledebouri) ), Ginger (Zingiberis Rhizoma crudus), forget-me-not (Natrii sulfas), control (Ziz) yphi Fructus, Salviae Radix, Mautan Radicis Cortex, Rehmanniae Radix, Mint Herba, Dioscoreae Rhizoma, Polyporus, Polygoni multiflori Radix, Gusi (Allii tube) Semen, Cassiae Semen, Lycii Fructus, Araliae cordatae Radix, Eucommiae Cortex, Hedyotis Herba, Saururus Herba, Artemisiaecapillaris Herba, Anemarrhen Rhizoma, Carthami Flos, Astragali Radix, Lycopodium, Ginkgonis Folium, Polygonati Rhizoma, Nelumbinis Semen, Fossilia ossis Mastodi, Cortexi radics ), Achyranthis Radix, Rehmanniae Radix preparata, Perillae Semen, Thujae Semen, Malt (Hordei Fructus germinatus), Cuscutae Semen, Mordaedae Radix, Sea Song (Pini koraiensis) Radix) and the like can be used alone or in combination.

The composition of the present invention may be administered in various parenteral formulations during actual clinical administration, and solid preparations include tablets, pills, powders, granules, capsules, and the like. In addition to water, liquid, and paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. Specifically, preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like. In addition, calcium or vitamin D ₃ may be added to enhance the efficacy of the treatment. Such compositions may be presented in unit-dose (single) or multi-dose (several) containers, such as sealed ampoules and vials, and immediately before use, sterile liquid carriers such as injectable water. Can be stored under freeze-drying conditions requiring only the addition of. Immediate injection solutions and suspensions can be prepared from sterile powders, granules and tablets.

The formulations of the present invention can be applied differently depending on the age, sex, condition of the subject, the absorption of the active ingredient in the body, the inactivation rate and excretion rate, the drug used in combination. The invention also includes formulations of dosage units. The formulations are present in individual dosage forms, such as tablets, coated tablets, capsules, pills, suppositories, and ampoules, wherein the amount of active compound in the drug corresponds to the fraction or multiple of the individual dosage. Dosage units may contain, for example, one, two, three or four times the individual dosage, or 1/2, 1/3 or 1/4 times. The individual dosages preferably contain an amount in which the active compound is administered at one time, which usually corresponds to all, 1/2, 1/3 or 1/4 times the daily dosage.

As used herein, the term "extract" refers to an active ingredient isolated from natural products. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or any form formulated using the same.

In a specific embodiment of the present invention, ethanol extract of Sculpture killed 99.7% of A549 non-small cell lung cancer cells at 100 μg / ml and the EC ₅₀ (half maximal effective concentration) was 14.6 μg / ml. The above results demonstrate that the extract of the present invention has excellent killing activity of A549 non-small cell lung cancer cells and further has lung cancer treatment and prophylactic activity.

As used herein, the term "prevention" means any action that inhibits or delays the development of lung cancer by administration of the composition.

As used herein, the term "treatment" means any action that improves or advantageously changes the symptoms of lung cancer by administration of the composition.

Extract which is a flake in the present invention can be extracted and used using water, an organic solvent, or a mixed solvent thereof. Preferably it is extracted using an organic solvent, in particular ethanol. The extracted solution can be used directly or can be concentrated and / or dried. When extracted with an organic solvent, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof may be used and extracted by room temperature or warming under conditions where the active ingredient of the herbal medicine is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and loss of the active ingredient of the drug may vary, so select an appropriate organic solvent. The extraction method is not particularly limited, and examples thereof include cold needle extraction, ultrasonic extraction, reflux cooling extraction, and the like. Filtration is a process of removing the suspended solid particles from the extract, it may be used to filter the particles using cotton, nylon or the like, or may be used, such as ultrafiltration, cryofiltration, centrifugal separation, but is not limited thereto.

Concentration of the extract may be used, such as concentrated under reduced pressure, reverse osmosis concentration. The drying step after concentration includes freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying, and the like. If desired, a process of grinding the final dried extract may be added.

In addition, the extract can perform an additional fractionation process. Preferably, the extract is suspended in distilled water to obtain a nonpolar solvent soluble layer by extraction and separation with a nonpolar organic solvent such as hexane, ether, dichloromethane, chloroform, ethyl acetate, or a mixed solvent thereof. It can be used by concentrating and / or drying it.

In the present invention, the term "pharmaceutically acceptable salts" means salts derived from pharmacologically or physiologically acceptable inorganic acids, organic acids and bases. Examples of suitable acid include hydrochloric acid, bromic acid, sulfuric acid, nitric acid, perchloric acid, fumaric acid, maleic acid, phosphoric acid, glycolic acid, lactic acid, salicylic acid, succinic acid, toluene-p-sulfonic acid, tartaric acid, acetic acid, citric acid, methanesulfonic acid, Formic acid, benzoic acid, malonic acid, naphthalene-2-sulfonic acid, benzenesulfonic acid, and the like. Salts derived from suitable bases may include alkali metals such as sodium, alkaline earth metals such as magnesium, ammonium and the like.

The pharmaceutical composition for preventing and treating lung cancer diseases of the present invention comprises 0.1 to 50% by weight of the extract or compound based on the total weight of the composition. In addition, the composition does not increase the efficacy, but may include additional ingredients that are commonly used in the pharmaceutical composition to improve the smell, taste, time and the like. In addition, the composition adds inorganic and organic additives such as vitamins B1, B2, B6, C, E, niacin, carnitine, betaine, folate pantothenic acid, biotin, zinc, iron, calcium, chromium, magnesium, and mixtures thereof. It can be included as. In addition, the composition may include a substance having a therapeutic activity against lung cancer, used alone or previously used.

As used herein, the term “patient” refers to humans, horses, sheep, pigs, goats, camels, who have disease caused by lung cancer and its direct and indirect causes, and whose symptoms may be improved by administering the composition of the present invention. Means antelope, dog and other animals. By administering to a patient a composition comprising an extract which is a fragment of the present invention, the above-mentioned lung cancer can be effectively prevented and treated. The composition of the present invention can be administered in parallel with existing lung cancer therapeutics.

As used herein, the term "administration" means introducing a predetermined substance into a patient by any suitable method, and the route of administration of the composition of the present invention is oral or parenteral via any general route as long as the target tissue can be reached. May be administered. In addition, the composition may be administered by any device in which the active agent may migrate to the target cell.

The composition of the present invention is administered in a pharmaceutically effective amount. As used herein, the term “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dose level refers to a patient's sexually transmitted disease, age, severity, and drug activity. , Drug sensitivity, time of administration, route of administration and rate of release, duration of treatment, factors including concurrently used drugs, and other factors well known in the medical arts. The compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. It may be single or multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art. The method of administering the composition comprising the extract or compound prepared according to the preparation method of the present invention is preferably oral administration or intravenous administration. In general, when the effective dose is oral administration, it is usually 1 to 1 time per adult. 500 mg / kg is preferred, and in the case of intravenous administration, 1 to 100 mg / kg is preferred, and may be administered 2-3 times a day. Dosage levels for a particular patient may vary depending on sex, age, health condition, diet, time of administration, method of administration, drug mixture, the condition of the patient, and the extent of the onset of neurological disease.

In addition, the present invention provides a functional food for preventing lung cancer containing Gleditsiae Semen ethanol extract containing a food supplement acceptable food supplement as an active ingredient.

Functional food of the present invention preferably contains 0.1 to 5% by weight, more preferably 1% by weight of the ethanol extract relative to the total weight. Functional food of the present invention is not particularly limited in the formulation, for example, dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, Various soups, beverages, tea, drinks, alcoholic beverages and vitamin complexes, etc., and includes all of the health food in the usual sense.

In the present invention, "functional food" means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to Act No. 6767 of the Health Functional Food Act, and "functional" refers to the structure of the human body And ingestion for the purpose of obtaining nutrients for function or for obtaining useful effects in health uses such as physiological actions.

In the present invention, as a result of injecting the extract of the present invention into A549 non-small cell lung cancer cells, it was confirmed that A549 non-small cell lung cancer cells were killed (see FIG. 1 ). Therefore, it can be seen that the extract extract is a fragment of lung cancer treatment effect. Thus, in the present invention, the present invention was completed by preparing a functional food for lung cancer prevention containing the extract of the flakes as an active ingredient (see Preparation Example 2 ).

As discussed above, the extract of the present invention inhibits the growth of lung cancer cells and induces apoptosis. Therefore, the composition for treating lung cancer according to the present invention will be very effective in the treatment of lung cancer patients.

1 is a result of Alamar Blue analysis to determine the effect of the introduction of sculpting on the growth of lung cancer cells in human lung cancer cells A549 non-small cell lung cancer cells, X-axis is the concentration of the extract of the fragment, Y-axis is alive Survival rate of human lung cancer cells.

Hereinafter, the present invention will be described in more detail based on the following examples. It should be noted, however, that the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The present invention is not limited to the following examples. Will be apparent to those skilled in the art to which the present invention pertains.

<Example 1> Preparation of the extract flakes

3 kg of flakes (made in China) purchased from Seoul medicinal herb were dried and ground for 5 days at the shade and room temperature. The ground flakes were immersed in 30 L of 95% ethanol and extracted at 50 ° C. for 24 hours. This was filtered through filter paper, dried and concentrated under reduced pressure of 45 ° C. to obtain 419 g of the total extract, and stored at −20 ° C.

Example 2 Effect of Sculpture Extract on Lung Cancer Cell Growth

In order to determine the effect of the extract extract of Example 1 on the growth of lung cancer cells, A549 non-small cell lung cancer cells, human lung cancer cells were treated with ethanol extract of the flakes for 48 hours and subjected to Alamar Blue analysis. The Alamar Blue assay is a modified form of the MTT assay, in which a specific enzyme degrades a living cell and then measures the fluorescence intensity of the product as the compound breaks down to determine the relative number of living cells after treatment. I am an experimental method. It will be described in more detail below.

<2-1> Preparation and Treatment of Human Lung Cancer Cell Lines

A549 cells, a lung cancer cell line used in the present invention, were distributed from the Korean Cell Line Bank (KCLB) and used for experiments. Specifically, A549 lung cancer cells RPMI 1640 medium (Robwell Park Memorial) containing 10% FBS (fetal bovine serum, fetal bovine serum) (Welgene) and 25 mM HEPES (4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid) Subculture was performed in a floating culture medium researched and developed by the Institute.

<2-2> Cell growth inhibition measurement of A549 non-small cell

The extract extracted in Example 1 was confirmed the effect of inhibiting the growth of lung cancer cells A549 non-small cell. Specifically, 1.4 X 10 ³ A549 lung cancer cells were injected into each well in a 96 well plate, cultured for 24 hours, and then the fragments dissolved in DMSO (dimethyl sulfoxide). When the ethanol extract was treated at concentrations of 0 to 100 μg / ml (specifically, concentrations of 0, 3.125, 6.25, 12.5, 25, 50, and 100 μg / ml, respectively) for 48 hours, the degree of inhibition of cell growth was confirmed. (Table 1). After treatment with each concentration of extract, 20 μl of Alamar Blue reagent was added to 0.2 ml of cell culture filled in each well in a 96-well plate, and the plates were incubated for 2 hours in an incubator. The plate was slowly shaken to evenly react the cells in each well, and the intensity of fluorescence was measured at 590 nm with a fluorescence photometer (Fluorescence Microplate Reader; Molecular Devices Corp.) while irradiating irradiated light at a wavelength of 544 nm. Survival is shown in FIG.

Carving Concentration Lung Cancer Cell Survival (Average) Standard Deviation 0 μg / ml 1,000 0.000 3.125 μg / ml 0.900 0.069 6.25 μg / ml 0.799 0.067 12.5 μg / ml 0.702 0.023 25 μg / ml 0.031 0.003 50 [mu] g / ml 0.002 0.002 100 μg / ml 0.003 0.001

As a result, as shown in Table 1 and Figure 1, the higher the concentration of sculptor treated lung cancer cell growth was reduced, from which it can be seen that sculptor has a lung cancer treatment effect. Ie 10.0% at 3.125 μg / ml, 20.1% at 6.25 μg / ml, 28.8% at 12.5 μg / ml, 96.7% at 25 μg / ml, 99.8% at 50 μg / ml, 99.7% at 100 μg / ml Lung cancer cells were killed. In addition, an EC ₅₀ (half maximal effective concentration) was determined to be 14.6 μg / ml. Table 1 above describes the relative cell numbers of lung cancer cells after 48 hours according to the treatment concentration of each fragment based on the number of survival rates of lung cancer cells of the control group that was not treated with fragment. As such, the extract, a fragment of the present invention, demonstrates good A549 non-small cell lung cancer cell killing activity and further has lung cancer treatment and prophylactic activity.

Example 3 Acute Toxicity Test by Extract

Sculpture used in the present invention was widely used as a medicinal herb was determined that there is no problem in the stability, but the oral and intraperitoneal toxicity experiments were carried out to confirm this.

Acute toxicity test was performed using 6-week-old SPF SD rats. Two animals per group were suspended orally administered at a dose of 5 g / kg, each of the extracts of the fragment of Example 1 of the present invention suspended in 0.5% methylcellulose solution. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed, and hematological and hematological examinations were performed.

As a result, there were no clinical symptoms or deaths in all animals treated with the test substance, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc. As a result, all of the extracts of the fragments did not show toxic changes up to 5 g / kg in rats, and the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance of 5 g / kg or more.

Preparation Example 1 Preparation of Lung Cancer Therapeutic Agent Containing the Extract of Sculpture as an Active Ingredient

The present inventors have confirmed that the lung cancer treatment effect of the extract sculpted through the above examples to prepare a lung cancer treatment containing the extract sculpted as an active ingredient as follows. In addition, the preparation of the following therapeutic agent can be used for the application of not only a therapeutic agent but also a health food.

<1-1> soft gelatin capsules containing flake extract

Sculpture Extract 20%

Vitamin C 4.5%

Vitamin D ₃ 0.001%

Manganese Sulfate 0.1%

10% wax

Palm oil 25%

Safflower Seed Oil 30.399%

 <1-2> Preparation of Intravenous Formulation Containing Extract of Sculpture

Carrot Extract 0.2%

Mannitol 0.3%

Saline 9.5%

<1-3> tablet containing extract of flakes

Carving Extract 35%

Vitamin C 10%

Vitamin D ₃ 0.001%

Manganese Sulfate 0.1%

Crystalline cellulose 25.0%

Lactose 17.999%

Magnesium Stearate 2%

Preparation Example 2 Preparation of Functional Foods Containing Extracts of Flakes as Active Ingredients

The inventors have confirmed that the extract extract is excellent in the lung cancer treatment activity through the above Example to prepare a functional food containing it as an active ingredient as follows.

<2-1> Preparation of Drink

522 mg of honey

Chioctosanamide 5 mg

Nicotinamide 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Carving Extract 0.48 ~ 1.28 mg

200 ml of water

A beverage was prepared using the above-mentioned composition and content by a conventional method.

<2-2> production of chewing gum

Gum base 20%

Sugar 76.36-76.76%

Sculpture Extract 0.24 ~ 0.64%

1% fruit flavor

Water 2%

Chewing gum was prepared using the above-mentioned composition and content by a conventional method.

<2-3> Preparation of Candy

Sugar 50-60%

Starch syrup 39.26 ~ 49.66%

Sculpture Extract 0.24 ~ 0.64%

Orange flavor 0.1%

The composition and the content of the candy were prepared using a conventional method.

<2-4> Preparation of Biscuits

Force Class 1 88 kg

Gravity First Class 76.4 ㎏

16.5 kg

2.5 kg of salt

2.7 kg of glucose

Palm shortening 40.5 kg

5.3 kg of ammo

Medium kg 0.6 kg

0.55 kg sodium bisulfite

Rice flour 5.0 kg

Vitamin B1 0.003 kg

0.003 kg of vitamin B2

Milk Flavor 0.16 ㎏

71.1 kg of water

Whole milk powder 4 kg

Substitute powder 1 kg

0.1 kg of calcium phosphate

Spray salt 1 kg

25 kg of spray oil

0.2 ~ 0.5 kg of flakes extract

Biscuits were prepared using conventional methods with the above composition and content.

<2-5> Preparation of Ice Cream

Milkfat 10.0%

Non-fat solids 10.8%

Sugar 12.0%

Starch syrup 3.0%

Emulsifying stabilizer (span) 0.5%

Spices (Strawberries) 0.15%

Water 63.31 ~ 62.91%

Sculpture Extract 0.24 ~ 0.64%

Ice cream was prepared using conventional methods using the above composition and content.

<2-6> manufacturing of chocolate

Sugar 34.36-34.76%

Cocoa Butter 34%

Cocoa Mass 15%

Cocoa Powder 15%

Lecithin 0.5%

0.5% vanilla

Sculpture Extract 0.24 ~ 0.64%

With the above composition and content, chocolate was prepared using conventional methods.

On the other hand, the specific scope of the present invention is defined by the claims rather than the embodiments described above, all changes and modifications derived from the meaning and scope and equivalent concepts of the claims to the scope of the invention It should be interpreted as including.

Claims (8)

A composition for preventing and treating lung cancer containing Gleditsiae Semen ethanol extract as an active ingredient.
According to claim 1, wherein the ethanol extract is a composition for preventing and treating lung cancer, characterized in that extracted for 24 hours at 50 ℃.
The composition for preventing and treating lung cancer according to claim 1 or 2, wherein the ethanol extract is dried and concentrated at 45 ° C under reduced pressure.
The composition for preventing and treating lung cancer according to claim 1 or 2, wherein the ethanol is 95%.
The composition for preventing and treating lung cancer according to claim 1, wherein the composition comprises a pharmaceutically acceptable carrier or diluent.
According to claim 1, wherein the lung cancer lung cancer prevention and treatment composition, characterized in that the non-small cell lung cancer.
The method of claim 6, wherein the non-small cell lung cancer is caused by a carcinoma selected from the group consisting of adenocarcinoma, squamous cell carcinoma, and large cell carcinoma. And therapeutic compositions.
Functional food for lung cancer prevention containing Gleditsiae Semen ethanol extract containing food acceptable food supplement additives as an active ingredient.

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