KR20120071780A - Composition for anti-obesity - Google Patents

Abstract

PURPOSE: An anti-obesity composition containing a Canavalia gladiata fermentation is provided to remarkably lower body weight. CONSTITUTION: An anti-obesity composition contains: a fermentation prepared by sealing a mixture of Canavalia gladiata and an ethanol solution and fermenting at room temperature(20-30°C) for 50-70 days; or a fermentation which is prepared by sealing the mixture at room temperature for 50-70 days, and maturing at low temperature(3~10°C) for 40-50 days. The composition additionally contains Zanthoxylum ailanthoides leaf extract. The ethanol solution contains 20-40%(v/v) of ethanol. The composition is a pharmaceutical or food composition. A composition for diet contains the fermentation as an active ingredient.

Description

Anti-obesity composition {Composition for Anti-obesity}

The present invention relates to an anti-obesity composition.

Recently, the obese population is rapidly increasing due to the improvement of living standards according to economic development, lack of exercise due to busy living environment, and excessive intake of nutrition. The proportion of obese populations in Korea is growing rapidly from 11.7% in 1995 to 29.4% in 2001 and 18.0% in 1995 to 32.6% in 2001 (Ministry of Health and Welfare, 2007).

Obesity is an abnormal increase in adipose tissue due to an imbalance in calorie intake and consumption [Kopelman PG. Neuroendocrine function in obesity. Clinical Endocrinology 28: 675-689, 1998; Clinical Obesity (eds. Kopelman PG, Stock MJ) 248-89 (Blackwell Science, Oxford 1998) .Clinically, the BMI (Body Mass Index) defines 25.0 to 30.0 as overweight and is overweight. define.

Obesity persists as a cause of diseases such as hypertension, arteriosclerosis, diabetes, fatty liver, gallstones, gout, and especially in obese women, endometrial cancer, cervical cancer, ovarian cancer, breast cancer, etc. In addition, in mortality, obesity is reported to be 1.3 times higher than normal weight (American Cancer Society, 2003).

Anti-obesity agents generally fall into three categories: appetite suppressants, energy metabolism accelerators and digestive absorption inhibitors.

Reductil (Abbott, USA) is a representative anti-obesity agent using a pharmacological mechanism that suppresses appetite.Exorise ™ (Aco Pharma, Inc.) is a representative anti-obesity agent using a pharmacological mechanism that promotes energy in the body. France), and a representative obesity treatment agent using a pharmacological mechanism that suppresses digestion and absorption of fat is Xenical ™ (Roche Pharmaceuticals, Switzerland).

Recently, various natural products such as plants have been reported for the purpose of suppressing and preventing obesity. As such natural products, for example, mixtures of hot water extracts of pine needles, organolpi, goji berry and ginseng (domestic patent registration No. 470204), sandderock extract (domestic patent registration No. 589672), bokbunja extract (domestic patent registration No. 466580), Plant extract (587398) etc. are mentioned.

The present invention discloses an anti-obesity composition using soybean fermentation.

An object of the present invention is to provide an anti-obesity composition.

The specific object of the present invention will be presented below.

As confirmed in the following Examples and Experimental Examples, the present invention, when the soybean fermented product was fed to the experimental animal with a high fat diet in the animal experiment, the increase in body weight is significantly lower than that of the experimental animal fed only the high fat diet. It is done by confirming the loss. The fermented soybeans used in the experiment was obtained by mixing the soybeans with 30% ethanol, sealing them, and fermenting them at room temperature (20 ~ 30 ℃) for 60 days at room temperature.The fermentation broth was sealed at low temperature (3 ~ 10 ℃) for 45 days. It is obtained by aging for days.

The anti-obesity composition of the present invention is provided based on the results of these experiments, and (I) a fermentation product obtained by fermenting at room temperature (20-30 ° C.) for 50 to 70 days by sealing a mixture of soybean and ethanol aqueous solution, or (II) Sealing a mixture of aqueous soybean ethanol solution, fermented at room temperature (20-30 ° C.) for 50-70 days, and then aging for 40-50 days at low temperature (3-10 ° C.) while maintaining the sealed state. It is characterized by including the fermented product as an active ingredient.

In the present specification, the term "obesity" refers to a state in which fat tissue is abnormally increased, whether it is caused by genetic factors or obesity due to environmental factors, and obesity according to the division of body mass index (BMI is 30.0 or more). ) And overweight (if your BMI is 25-30).

In addition, in the present specification, the "active ingredient" refers to a component that can exhibit the activity alone or in combination with a carrier having no activity in itself.

In addition, in the present specification, the term "anti-obesity" is meant to include the reduction of body fat and / or weight loss, including the prevention of obesity, the treatment of obesity.

In addition, in this specification, a "fermented substance" is meant to include the fermentation stock solution after completion | finish of fermentation or fermentation and maturation, the filtrate of this fermentation stock solution, and the liquid or solid concentrate obtained by concentrating this filtrate.

Fermented soybeans as an active ingredient in the anti-obesity composition of the present invention is a soybean ( Canavalia) gladiata ) is obtained by mixing, sealing and fermenting an aqueous solution of ethanol, where the aqueous solution of ethanol is in the range of 20-40% (v / v) where the preferred ethanol content is a mixture of ethanol and water, where% means volume percentage. Equal to). It is because if less than 20% unintended fermentation may proceed by the various bacteria, and if more than 40% fermentation may not proceed or the fermentation may proceed very slowly. Although not shown in the following Examples and Experimental Examples, the anti-obesity activity was very insignificant when fermented with 50% ethanol aqueous solution, and when it was fermented with 13% ethanol aqueous solution, it caused an undesirable smell in the odor of the fermentation broth. .

In addition, in the preparation of fermented soybeans, which are included as an active ingredient in the anti-obesity composition of the present invention, the fermentation-maturation period is determined in relation to the fermentation-maturation temperature, but the fermentation period is 70 days when the fermentation temperature is 20 ° C. Preferably, the fermentation period is about 50 days if the fermentation temperature is 30 ℃. In addition, the ripening period is preferably 50 days when the ripening temperature is 3 ℃, the ripening period is preferably 40 days when the ripening temperature is 10 ℃. If the fermentation temperature and the fermentation period is out of the above range, unintended fermentation and ripening proceeds, or the fermentation and maturation degree is low, so that the desired effect of fermentation and ripening cannot be obtained.

The anti-obesity composition of the present invention may include any effective amount (effective amount) according to the use, formulation, formulation purpose, etc. as long as the effective ingredient can exhibit the activity for improving obesity, the usual effective amount is based on the total weight of the composition Will be determined within the range of 0.001% to 99.990% by weight. Here, the "effective amount" refers to the amount of the active ingredient that can induce the effect of improving obesity. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

Subjects to which the compositions of the invention can be applied (prescribed) are mammals and humans, in particular humans.

The anti-obesity composition of the present invention, in a preferred embodiment, is characterized in that it further comprises a buckthorn leaf extract in addition to the bean extract as an active ingredient.

The following experimental example shows that the anti-obesity activity is increased when the worm leaf extract is included.

In the above, the extract of the thorn tree is obtained by immersing the extract of the target worm ( Zanthoxylum ailanthoides ) in water, ethanol or a mixture thereof, which is an extraction solvent. It is meant to include liquid or solid concentrates.

In another aspect, the present invention (I) sealing the mixture of soybean and ethanol aqueous solution to ferment the product obtained by fermentation at room temperature (20 ~ 30 ℃) for 50 to 70 days, or (II) a mixture of aqueous soybean ethanol solution For fermentation at room temperature (20 ~ 30 ℃) for 50 to 70 days and then maintain the sealed state as it is, for a diet containing fermented product obtained by aging for 40 to 50 days at low temperature (3 ~ 10 ℃) as an active ingredient It relates to a composition.

As used herein, "diet" is defined as a condition in which a weight condition is not obese or overweight, but for which a reduction in weight / body fat is desirable or necessary for cosmetic or health purposes. Usually the composition for a diet of the present invention will be prepared for use for the beauty or health purposes of normal people.

In the composition for a diet of the present invention, with respect to the meaning of the fermented soybean, its effective amount, and the like, the above-described bar as described above with respect to the obesity improving composition of the present invention is effective.

The composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

Pharmaceutical compositions of the present invention, in addition to its active ingredients, include pharmaceutically acceptable carriers, excipients, and the like, oral formulations (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral formulations (sterile injectable aqueous solutions). Or oily suspensions), topical formulations (creams, lotions, ointments (semi-solid external preparations), microroemulsions, gels, pastes, transdermal agents (TTS) (such as patches, bandages, etc.), and the like.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) is not as toxic as applicable, without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, Solid lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, emulsifiers (such as TWEENS), wetting agents (Such as sodium lauryl sulfate), colorants, flavors, tableting agents, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions, and the like. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, when the pharmaceutical composition of the present invention is prepared with an aqueous suspending agent, suitable excipients are sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethylcellulose And suspending agents and dispersing agents such as sodium alginate and polyvinylpyrrolidone. Suitable excipients when prepared from injection solutions include Ringer's solution, isotonic sodium chloride, and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, preferably topically.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

The composition of this invention can be grasped | ascertained by food compositions, such as a functional drink, in a specific aspect.

The food composition of the present invention may include sweeteners, flavoring agents, bioactive ingredients, minerals, etc. in addition to the active ingredients.

Sweeteners may be used in amounts that give the food a suitable sweet taste, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavoring agents can be used to enhance the taste or aroma, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. In some cases, synthetic flavoring agents may be used, and synthetic flavoring agents may include esters, alcohols, aldehydes, terpenes, and the like.

As the physiologically active substance, catechins such as catechin, epicatechin, gallocatechin, epigallocatechin, vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, riboflavin, and the like can be used.

As minerals, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc and the like can be used.

In addition, the food composition of the present invention may contain a preservative, an emulsifier, an acidulant, a thickener, and the like, in addition to the sweetener.

Such preservatives, emulsifiers and the like are preferably added and used in very small amounts as long as the use to which they are added can be achieved. By trace amount is meant numerically expressed in the range of 0.0005% to about 0.5% by weight based on the total weight of the food composition.

Examples of preservatives that can be used include sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), and the like.

Emulsifiers that can be used include acacia gum, carboxymethylcellulose, xanthan gum, pectin and the like.

Examples of acidulants that may be used include lead acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like. Such acidulant may be added so that the food composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing taste.

Thickeners that can be used include suspending implements, sedimenters, gel formers, swelling agents and the like.

As described above, the present invention can provide an anti-obesity composition. The anti-obesity composition of the present invention can be commercialized into drugs, foods and the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Small beans Fermented  Produce

Example 1 Small Beans Fermented product Preparation Example 1

500 g of soybeans were mixed with 1.5 L of 30% ethanol (30% ethanol aqueous solution; the same as below), put into a container, sealed, and fermented at room temperature (20-30 ° C.) for 60 days to obtain a soybean fermentation broth. The fermentation broth was filtered through a 2 μm filter paper and then lyophilized to obtain a solid fermentation product.

Example 2 Fermented Soybean Leaf Preparation Example 2

The fermented soybean fermentation broth obtained in <Example 1> was kept at a low temperature (3-10 ° C.) for 45 days to prepare a ripe soybean fermentation broth. The fermentation broth was filtered through a 2 μm filter paper and then lyophilized to obtain a solid fermentation product.

< Comparative example > Small beans  Preparation of extract

500 g of bean beans were immersed in 1.5 L of 70% ethanol (70% ethanol aqueous solution) and extracted with stirring for 24 hours. After extraction, the extract was filtered with 2 탆 filter paper and then lyophilized to obtain a solid extract.

< Reference Example > Buckthorn  Preparation of Leaf Extract

500 g of the leaf flakes were immersed in 1.5 L of 70% ethanol (70% ethanol aqueous solution) and extracted with stirring for 24 hours. After extraction, the extract was filtered with 2 탆 filter paper and then lyophilized to obtain a solid extract.

< Experimental Example > Experiment on Obesity Improvement Activity

Sprague-Dawley rats weighing 200-250 g were purchased from Korean experimental animals and used in the experiment after being purified in a nursery environment for two weeks with sufficient feed and water.

Experimental animals were randomly divided into 6 groups of 5 (n = 5) and fed the normal diet (rat chow (Samyangsa)) with water for the normal group and the high-calorie diet (1.5% cholesterol (Sigma for the normal diet) for the obese control group. US, USA), 0.5% cholic acid (Sigma, USA) and 25% lard (lard, Sigma, USA) were fed with water, and experimental groups (experimental groups I to IV) High calorie diets and samples were fed with water. The sample is a high-calorie diet of 100 wt% of the fermented products of Examples 1 and 2 (Experimental Groups I & II, respectively), the extract of Comparative Example (Experimental Group III), or the mixture of the fermented products of Example 2 and the extract of Reference Example (Experimental Group IV). 2 parts by weight of the diet was mixed and fed. Diet and water were taken freely.

In order to exclude the deviation between the test animals, one dog was separated and reared for 8 weeks. The breeding environment was maintained at a temperature of 23 ± 3 ° C., a humidity of 55 ± 15%, and a night and day cycle for 12 hours.

Body weights were measured using animal scales at the beginning of the experiment and after 8 weeks of breeding to determine their changes.

The results are shown in the following [Table 1], and the statistical treatment was Student's t-test, and the significance was verified at P <0.05 level.

Change in body weight of test animal (g) division Weight change Normal 132.52 ± 7.48 Obesity Control 196.73 ± 5.82 † Experiment group I (fermented product administration group of Example 1) 162.38 ± 11.64 * Experiment group II (fermented product administration group of Example 2) 154.26 ± 7.38 * Experiment group Ⅲ (extract administration group of the comparative example) 178.39 ± 4.58 * Experimental group IV (the same amount of mixture administration of Example 2 and Reference Example) 142.82 ± 5.83 * †: Significant compared to normal group.
*: Significant compared to the obese control group.

As can be seen in Table 1, both the soybean extract (experimental group Ⅲ) and the soybean fermented product (experimental group I & II) showed an improvement in obesity, but the soybean fermented product had a higher obesity improving activity than the soybean extract, The fermented soybeans of Example 2 (Experimental Group II) have high obesity improving activity. In particular, the fermented product of <Example 2> was mixed with administration of the leaf extract of the tree, the obesity improvement activity was the highest.

Claims (7)

(I) a fermentation product obtained by sealing a mixture of soybeans and an ethanol aqueous solution and fermenting at room temperature (20 to 30 ° C.) for 50 to 70 days, or
(II) Sealing the mixture of soybean and ethanol aqueous solution to ferment at room temperature (20 ~ 30 ℃) for 50 ~ 70 days, and then aged for 40 ~ 50 days at low temperature (3 ~ 10 ℃) while maintaining the sealed state Anti-obesity composition comprising fermented product as an active ingredient.
The method of claim 1,
The composition is an anti-obesity composition, characterized in that it further comprises a buckthorn leaf extract.
The method of claim 1,
The fermented product (I) is a fermentation product obtained by fermenting a soybean for 30 days at room temperature (20 ~ 30 ℃) by sealing a mixture of 30% aqueous ethanol solution,
The fermentation product of (II) is fermented for 60 days at room temperature (20 ~ 30 ℃) by sealing the mixture of 30% ethanol aqueous solution of the soybeans, and then maintain the sealed state for 45 days at low temperature (3 ~ 10 ℃) Characterized in that it is a fermented soybean fermented by aging for a while
Anti-obesity composition.
The method of claim 1,
The ethanol aqueous solution is an anti-obesity composition, characterized in that the ethanol content of 20% (v / v) to 40 (v / v)%.
The method of claim 1,
The composition is an anti-obesity composition, characterized in that the pharmaceutical composition.
The method of claim 1,
The composition is an anti-obesity composition, characterized in that the food composition.
(I) a fermentation product obtained by sealing a mixture of soybeans and an ethanol aqueous solution and fermenting at room temperature (20 to 30 ° C.) for 50 to 70 days, or
(II) Sealing the mixture of soybean and ethanol aqueous solution to ferment at room temperature (20 ~ 30 ℃) for 50 ~ 70 days, and then aged for 40 ~ 50 days at low temperature (3 ~ 10 ℃) while maintaining the sealed state Diet composition comprising the fermented product as an active ingredient.