KR20120060194A - Composition having protective function from fatty liver using an extract of dropwarts as active ingredient and Method for manufacturing the same - Google Patents

Abstract

PURPOSE: A composition containing Oenanthe javanica(BLUME.) extract and a method for preparing the same are provided to prevent and relieve fatty liver. CONSTITUTION: A method for preparing a composition containing OENANTHE javanica(BLUME.) extract for relieving fatty liver comprises: a step of drying and pulverizing whole plant or upper part of Oenanthe javanica(BLUME.); a step of adding extraction solvent and refluxing; and a step of filtering or concentrating the extract. The extraction solvent includes water or low carbon number alcohol of 1-4 carbon atoms.

Description

Composition having protective function from fatty liver using an extract of dropwarts as active ingredient and Method for manufacturing the same}

The present invention relates to a composition for releasing fatty liver comprising a buttercup extract as an active ingredient and a method for preparing the same, and more particularly, to prevent and cure fatty liver with a hydrothermal extract extracted from natural buttercup and n-butanol fraction thereof. It relates to a composition and a method for producing the same.

In general, as eating patterns gradually become westernized, the intake of cereals rapidly decreases, and the intake of fats, especially animal fats, increases, and the incidence of various chronic metabolic diseases such as hyperlipidemia, arteriosclerosis, fatty liver, and diabetes increases. It is a serious social problem.

Of these, fatty liver can impair the liver's function of metabolism, detoxification, coagulation of blood and regulation of blood volume by making foods absorbed from the stomach into necessary components for the body, but liver is often ' As long as it is almost impossible to cure a certain disease to the extent that it is called the 'silent organ', the importance of preventive treatment is increasing because it is very difficult to treat the disease.

The liver is the largest organ of about 2% of the body weight, plays a central role in metabolism, metabolism of nutrients, detoxification of drugs and toxins, bile formation, filtration of blood from the intestine, urea synthesis , Inactivation of various hormones, production of plasma proteins such as immunoglobulins, etc., which is responsible for life support, particularly in terms of fat metabolism, β-oxidation and acetoacetate of fatty acids. ), Lipid synthesis (lipoprotein), cholesterol (cholesterol) and phospholipid (synthesis), and fat synthesis from sugar and protein.

The excess accumulation of fat in these livers is called fatty liver, and if fatty liver persists for a long time, hepatitis (Charlton et al .: Hepatology 35: 898-904, 2002), liver fibrosis or cirrhosis (Farrell and Larter, 2006; Adams et al., J Hepatol 42 132-138, 2005), liver cancer (Bugianesi et al., Gastroenterology; 123: 134-140, 2002; Marrero et al., Hepatology 36: 1349-1354, 2002), as well as hypertension, hyperlipidemia, arteriosclerosis, insulin It is known to promote the development of various chronic diseases such as resistance (Pagano et al., Hepatology 35: 367-372, 2002), and excessive accumulation of fat in the liver is more than the amount of fat used or excreted in the liver. In cases (Donnelly et al., J Clin Invest 115: 1343-1351, 2005).

Recently, research and experiments have been conducted on many substances for preventing and eliminating fatty liver. However, the only effective substance for fatty liver is metadoxin as a medicine, and few substances with good effects and few side effects are rare. It is not developed.

On the other hand, Oenanthe javanica is a typical food that clears blood. It is a food that has been cherished in the court since ancient times. It is a representative alkaline food that is rich in minerals, and according to the agreement and herbal milk, parsley clears the head, smoothes the large intestine and small intestine, promotes metabolism, lowers fever and is effective for rheumatism. It is reported that it is effective in eggplant disease, and it is recognized that the effect of lowering blood pressure is one of the foods enjoyed by hypertensive patients, and it is also known to relieve constipation and neutralize or remove various toxic substances in the body.

These effects are mainly due to the various flavonoids contained in buttercups, such as Quercetin, Isorhamnetin, Hyperside, etc. Among them, Persicarin has recently been known as an active substance. It is known to have a very good hepatoprotective effect. The flavonoid component of the buttercup is known to have a hepatoprotective effect, an antimutagenic and antioxidant effect, a high blood pressure treatment effect as well as an excellent effect on eliminating hangovers after drinking. Vitamin C has already been fully tested for its functions, including fatigue and antioxidant effects.

Accordingly, the present inventors have confirmed that the composition comprising the extract extracted by hot water in hot water and the active ingredient extracted secondly with butanol again has the effect of preventing and eliminating fatty liver by inhibiting the accumulation of liver fat in a living body. Was completed.

The present invention has been made in order to solve the above-mentioned conventional problems, by extracting a specific ingredient of the natural food at a predetermined ratio, it is harmless to the human body to prevent fatty liver containing buttercup extract as an active ingredient without any side effects And it is an object to provide a composition to solve and a method for producing the same.

Method for producing a composition for preventing and releasing fatty liver containing the buttercup extract according to the present invention for achieving the above object, after drying the outpost or ground part of the apiaceae plant at room temperature for one week, the shaded A milling step of preparing apiaceae plants into milled products; An extraction step of adding an extraction solvent to the pulverized product pulverized in the pulverizing step and extracting it under reflux; And a hot water extract manufacturing step of preparing the buttercup hot water extract by filtration or concentration of the extract obtained in the extracting step. In the extracting step, the pulverized product and the extraction solvent are mixed in a weight ratio of 1:10, and The extract is refluxed at a temperature of 80 to 100 ° C. for 1 to 3 hours, and the extracting solvent is formed of one or more selected from the group consisting of water, a low alcohol having 1 to 4 carbon atoms, or a low alcohol.

Moreover, this invention provides the composition obtained by said method.

The present invention can be promoted to improve the health of the human body by showing the activity in the prevention and remedy of fatty liver without any side effects due to no harm to the human body through the water extract of the buttercup hot water extract and the n-butanol fraction of the specific ingredients of natural foods have.

1 is a diagram showing the change in body weight after breeding Sakura hot water extract, butanol extract and metadoxin for 6 weeks with high fat diet in Sprague-Dawley rats applied to the present invention
Figure 2 is an example showing the change in visceral fat amount after breeding Sprague-Dawley rats applied to the present invention for 6 weeks with a high-fat diet of Buttercup hot water extract, butanol extract and metadoxin
Figure 3 is a diagram of the changes in liver triglycerides and total cholesterol concentrations after breeding the Buttercup hydrothermal extract, butanol extract and metadoxin with high fat diet for 6 weeks in Sprague-Dawley rats applied to the present invention Example shown by
Figure 4 is a Sprague-Dawley rats applied to the present invention after breeding the Buttercup hydrothermal extract, butanol extract and metadoxin with a high fat diet for 6 weeks and stained liver tissue with hematoxylin and eosin Example showing changes in liver tissue and the degree of fat

Hereinafter, with reference to the drawings will be described in detail with respect to the fatty liver composition and the preparation method comprising a buttercup extract applied to the present invention as an active ingredient.

1 is an example showing the change in body weight after breeding Sakura hot water extract, butanol extract and metadoxin for 6 weeks in a Sprague-Dawley rat applied to the present invention with a high fat diet, Figure 2 is an embodiment showing the change in visceral fat amount after the breeding of hot water extract, butanol extract and metadoxine for 6 weeks with high fat diet in the sprag Dauri-based rats applied to the present invention, Figure 3 Fig. 4 is a diagram showing changes in liver triglycerides and total cholesterol concentrations after breeding the hot water extract, butanol extract, and metadoxin of Buttercup Dari rats applied with the present invention for 6 weeks with a high fat diet, and FIG. 4. The sprag dauri rats applied to the present invention for 6 weeks with a high fat diet of Buttercup hot water extract, butanol extract and metadoxin Yukhan the embodiment shown the degree of change and fat tissue of the liver for example, H. and stained with hematoxylin and eosin of liver tissue after.

Fatty liver prevention and remedy composition of the present invention is obtained by extracting the outpost or the ground part of the apiaceae plant with hot water, wherein the asteraceae plants Ostericum coreanum (Ostericum coreanum), Oenanthe javanica, Oe It includes all apiaceae plants, such as Nante stolonifera (Oenanthe stolonifera), looking at the hydrothermal extraction and butanol extraction process of the buttercup implemented by one embodiment of the present invention, as follows.

[Manufacturing Process of Hot Water Extract of Buttercup]

After cleaning the parsley cultivated in Pyongyang-ri, Cheongdo-eup, Cheongdo-gun, Gyeongbuk, Korea, dry it for 1 week at room temperature, take 600 g, crush it, add 6 liters of distilled water, extract it at reflux for 1 to 3 hours, and evaporate it to dryness. As a result of measuring the amount of extract, a yield of 27-31% was obtained from 164.0 to 185.8 g, and the analysis of the buttercup hydrothermal extract by HPLC showed that the content of persicarin, which is an indicator, was 0.23%. HPLC is Shimadzu LC-10A dual pump, SCL-10vp Controller, SIL-AF autoinjector, SPD-M 10Avp 253nm using a diode array detector (SPD-M 10Avp diode array detector) and outflow solvent at 6ml / min flow through Shim-pak CLC-ODS Effluent at Wavelength Detection, and to the outlet are prepared using distilled water as the solvent A mixture of methanol (methanol), B solution, and outlet using a concentration gradient of the two solvents.

Buttercup hot water extract as described above can be used to extract the active ingredient using water, organic solvents or mixed solvents thereof as the extraction solvent, using the outpost or ground portion of the apiaceae plant, or may be used by concentrating it in some cases, wherein The extraction solvent may be water, a low alcohol or dilute water having 1 (C1) to 4 carbon atoms (C4), but is not limited thereto. The low alcohol may be ethanol, methanol, n-butanol, isobutanol, Any one of n-propan and isopropanol may be used, but is not limited thereto, and preferably n-butanol may be used.

In particular, in the present invention, the buttercup is extracted by refluxing with water at 50 to 110 ° C, preferably at 80 to 100 ° C for 1 hour to 1 day, preferably 2 to 3 hours, to prepare a buttercup hydrothermal extract. After extraction with reflux, the content of persicarin, a marker, was analyzed by HPLC, and the content of persicarin was 0.23%.

[Manufacturing process of Buty Butanol Extract]

Buttercup butanol extract is prepared by adding the same amount of n-butanol to 1 liter of the Buttercup hot water extract and repeated extraction three times, collecting the extract solution in one place to concentrate and remove the solvent to fractionate n-butanol.

Here, the buttercup butanol extract has been described as an example of preparing a fraction by adding n-butanol to the buttercup hot water extract, but the solvent for fractionating the buttercup hot water extract is composed of n-hexane, methylene chloride, acetone, ethyl acetate and n-butanol Fractionation may be performed using one or more solvents selected from the group, and the preparation temperature of the fractions fractionated by using an additional solvent to the extract or extract may be 20 to 50 ° C., but is not limited thereto. Although not limited, it is preferable to extract within 10 to 1 days, and the extraction device may be a conventional extraction device, ultrasonic grinding extractor or fractionator, the extract thus prepared is hot air drying, reduced pressure drying or / and freezing Drying may remove the solvent, but is not limited thereto, and preferably Pressure can be dried to remove the solvent.

[Production Example of Pharmaceutical and Functional Health Food Using Buttercup Hot Water Extract]

Fatty liver reliever composition (hereinafter, referred to as `` parsley composition '') comprising the buttercup extract according to the present invention as an active ingredient includes a pharmaceutically acceptable carrier or a food pharmaceutically acceptable food supplement additive to prevent and resolve fatty liver. It can be used as a pharmaceutical and functional health food.

In the present invention, the buttercup composition may be used alone, but is not limited thereto. The buttercup composition may be used by additionally including a pharmaceutically acceptable carrier or excipient according to the formulation and purpose of use. Excipients including acceptable carriers or diluents, preservatives, stabilizers, wetting agents, emulsifiers, solubilizers, sweeteners, colorants, osmotic pressure-controlling agents and antioxidants can be used with conventional materials depending on the dosage form. , Wetting agents, disintegrating agents or surfactants may be used, and at this time, representative diluents or excipients may include water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paratin and physiological saline.

In addition, the buttercup composition may be administered orally or parenterally, and the formulation thereof may be used as a warning agent (PLASTERS), granules (GRANULES), lotions (LOTIONS), powders (POWDERS), syrups (SYRUPS), solutions LIQUIDS AND SOLUTIONS, AEROSOLS, OINTMENTS, FLUIDEXTRACTS, EMULSIONS, CAPSULES, or PILLS, but are not limited thereto.

In addition, the buttercup composition of the present invention may be preferably formulated using a method known in the art or a method described in Remington's Pharmaceutical Science (latest version), Mack Publishing Company, Easton, PA.

The dosage or amount of the buttercup composition is preferably used in consideration of the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate and severity of the disease, the dosage is usually 1 ~ 250mg / kg, frequency of administration can be administered once or several times a day, preferably in the case of adult (70kg) divided into 0.5 ~ 2g, 3 times or 4 times a day, the administration period is also particularly limited The buttercup composition may be used in addition to any food other than oral administration alone.

In addition, the buttercup composition of the present invention can be used as a functional health food or health food supplements, etc., because it can be taken for a long time without toxicity and side effects, wherein the health food may include drugs, food and beverages, etc. Specifically, there are various foods, candy, chocolate, beverages, gums, teas, vitamin complexes, health supplements, and the like, and may be used in the form of powders, granules, tablets, capsules, or beverages.

The amount of the buttercup composition added to the food or beverage is not particularly limited, but may be used in an amount of 1 to 80% by weight, preferably 1 to 20% by weight of the total food weight, and 0.1 to 10 g per 100 ml of the health beverage. Preferably, it can be used in the ratio of 0.5-2 g.

At this time, the functional health food may additionally include a food supplement acceptable food supplement with the buttercup composition of the present invention, for example, the liquid component used in the health beverage containing the buttercup composition is particularly limited It is not a matter of course, and may contain various flavors or natural carbohydrates, etc. as an additional ingredient as in a conventional beverage.

Examples of the natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and sugar alcohols such as xylitol, sorbitol, and erythritol are included, and the flavoring agent is a natural flavoring agent [taumatin, stevia extract (for example, rebaudio) Seed A, glycyrrhizin, etc.] or synthetic flavoring agents (saccharin, aspartame, etc.), and the ratio of the natural carbohydrate is 0.1 to 20 g, preferably 0.5 to 2 g per 100 ml of the health beverage composition. to be.

In addition, the functional health food is a variety of nutrients, vitamins, minerals (electrolytes), natural or synthetic flavors, colorants or fillers (such as cheese, chocolate), pectic acid or salts thereof, alginic acid or salts thereof, organic acids, protective properties It may contain colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, carbonates used in alcoholic or carbonated beverages, and the like, and may also contain flesh to produce natural fruit or vegetable beverages. Or it may be used in combination, the ratio of the additive is not particularly limited, but may be selected in the range of 1 to 20% by weight per 100% by weight of functional health food.

[Example showing the effect of hot water extract and Butanol extract on fatty liver]

In order to investigate the effects of Buttercup hot water extract and butanol extract on fatty liver in vivo , 6 weeks of high fat-cholesterol diet (HFCD) was used in Sprague-Dawley rats. As shown in Fig. 3, the 6-week high fat-cholesterol diet showed about 1.5 times higher liver triglyceride content and 3.5 times higher cholesterol than the normal chow group. It can be seen that the fatty liver model was successfully induced.

As a control drug, metadoxin, a drug currently used as a liver treatment such as fatty liver, is used, and the recommended dosage of the metadoxin is 500 mg once per adult and twice a day, as shown in FIGS. 1 and 2. The used buttercup extract was formulated by administering 0.5g of dry raw materials before the composition of the buttercup extract per 100g of dietary cholesterol, and metadoxin 15mg. The total number of rats used was 30 rats, and the high fat-fat after 6 weeks diet. Cholesterol diet group increased body weight by 19-30%, visceral fat 21-36% compared to normal diet group, and significantly higher body weight or visceral fat mass in high fat-cholesterol diet group compared to non-administrated group. It can be seen that there is no difference.

As shown in FIG. 3, the liver triglyceride concentration was measured 6 weeks after the administration of the extract of the buttercup with the high fat-cholesterol diet, but the hot water (HFCD-W) or butanol (HFCD-B) compared to the control (HFCD). In the experimental group treated with the extract, 13% and 14% lower, respectively, while the control group, metadoxin-ingested group (HFCD-M), showed a decrease of 8%, and the buttercup extract group was 58% and 75% higher than the control group. It can be seen that the effect is excellent.

In addition, the total cholesterol content of the liver was decreased by 13% in the hot water extract, but the metadoxin intake group (HFCD-M), a control drug group, showed a decrease rate of 18%. It is more effective than metadoxin in, and metadoxin may be more effective in reducing total cholesterol concentration.

As shown in FIG. 4, the results of the observation of the liver biopsy at 200 times magnification showed that the high fat-cholesterol diet group showed a lot of fat in the tissue, but the buttercup extract and metadoxin administration group showed much improvement. There is a number.

[Observation of tissue changes according to the administration of Buttercup hot water extract and n-butanol fraction thereof]

In order to observe the occurrence of acute side effects from the administration of Buttercup hot water extract and its n-butanol fraction, the dose in the above example was increased by 10 times and administered to ICR mice in the same manner, and the brain, cerebellum, heart of the mouse The tissues of the liver, kidney, small intestine, pancreas and skeletal muscle were cut and examined, but no significant tissue changes were observed in each tissue compared to the normal control group.

Claims (5)

After drying the outpost or ground portion of the apiaceae plant for 1 week at room temperature, the milling step of manufacturing the dried apiaceae plant as a pulverized product;
An extraction step of adding an extraction solvent to the pulverized product pulverized in the pulverizing step and extracting it under reflux; And
It consists of a hydrothermal extract manufacturing step of producing a buttercup hot water extract by filtration or concentration of the extract obtained in the extraction step;
In the extraction step,
The pulverized product and the extraction solvent are mixed at a weight ratio of 1:10, and the mixture is refluxed for 1 to 3 hours at a temperature of 80 to 100 ° C,
The extraction solvent,
A method for producing a composition for releasing fatty liver, comprising a parsley extract as an active ingredient comprising at least one member selected from the group consisting of water, a low alcohol having 1 to 4 carbon atoms or a low alcohol. The method of claim 1,
Buttercup hot water extract prepared in the hydrothermal extract manufacturing step, the method for producing a composition for releasing fatty liver comprising a buttercup extract as an active ingredient, characterized in that the content of persicarin is prepared as 0.23%. The method of claim 1,
After the hydrothermal extract manufacturing step,
A fraction extraction step of preparing a fraction extract after mixing the hot water extract prepared by the hydrothermal extract preparation step and the fraction solvent; And
Further comprising: a drying step of drying the fraction extract extracted in the fraction extraction step;
The method of producing a composition for releasing fatty liver comprising the buttercup extract as an active ingredient, characterized in that the buttercup hydrothermal extract and the fraction solvent in the fraction extraction step by mixing the same amount fractions. The method of claim 3,
Fraction solvent used in the fraction extraction step,
Method for producing a composition for releasing fatty liver comprising a buttercup extract as an active ingredient, characterized in that at least one solvent selected from the group consisting of n-hexane, methylene chloride, acetone, ethyl acetate and n-butanol. A composition prepared using the method of any one of claims 1 to 4.

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