KR20120034485A - A disease control system with portable measuring apparutus - Google Patents

Abstract

PURPOSE: A disease diagnosing/managing system using a portable measuring device is provided to concentratedly diagnose/manage a disease using measured information. CONSTITUTION: A portable measuring device measures a concentration and whether pathogenic fungi exist in a tested material using an optical feature. The portable measuring device generates and transmits the measured result. A disease managing server(420) receives the measured result transmitted from the portable measuring device. The disease managing server stores the received measured result in a database. When a result test material made by analyzing the received measured result is infected, the disease managing server transmits a warning message according to preset information.

Description

A Disease Control System With Portable Measuring Apparutus}

The present invention relates to a disease diagnosis / management system using a portable measuring device, and more particularly, to conveniently and conveniently measure disease infection using a portable measuring device capable of measuring immunoaggregated scattered light and / or PDA embolism. In addition, the present invention relates to a disease diagnosis / management system using a portable measuring device that can centrally diagnose and manage a disease at a national or regional level by using measured information.

In the prior art, a sample for measurement (a test substance such as blood or secretion) is collected from a diagnosis target (human, animal, etc.) in diagnosing a disease or the like, and the collected sample (test substance) is used. It was configured to determine whether a particular disease was infected, and this measurement was possible only at a hospital, quarantine, or research institute equipped with separate measuring equipment.

Meanwhile, as biotechnology, nanotechnology, and IT technology have developed, biosensing technologies using diagnostic kits that respond to specific pathogens have been developed, and their utilization is gradually increasing. The diagnostic kit includes a specific substance (pathogen, etc.) contained in the test substance and a reactant causing a specific chemical / physiological reaction, and when the test substance is added to the diagnostic kit, the specific substance is determined according to the reaction status. It is configured to determine whether the inclusion of (ie, disease infection, etc.). However, even when performing a diagnosis using a diagnostic kit using such a bio-sensing technology, there is a problem that it is possible only at a hospital, quarantine, or research institute equipped with a separate fixed measuring device, and it is determined that the infection is accurately measured and infected. If there is a problem that the concentration can not be measured accurately.

On the other hand, due to the absence of a nationwide disease diagnosis / management system, there was a problem that integrated and immediate disease diagnosis and management could not be achieved in accordance with the prior art. In other words, transport the test material collected from the field to a hospital, quarantine office, research center, etc. with inspection / measurement equipment, determine whether the pathogen is infected by using fixed measuring equipment, and report and message by manpower according to the result. Due to the problems of the prior art that is configured to propagate, there was a problem that can not be immediately diagnosed and responded to the disease, and systematic disease information management.

In addition, according to the prior art, since a series of disease management processes such as diagnosis of disease infection, disease occurrence report, disease occurrence warning propagation are performed through separate and separate systems, efficient disease diagnosis and management cannot be achieved. There was a problem.

The present invention has been made to solve the problems described above, using a portable measuring device capable of immuno-aggregated scattering light measurement and / or PDA embolism measurement to conveniently and conveniently measure the disease infection, and measure the measured information The purpose of the present invention is to provide a disease diagnosis / management system using a portable measuring device that can centrally diagnose and manage diseases of a nation or a certain regional unit.

In order to achieve the above object, according to a preferred embodiment of the present invention, a portable measuring device for generating and transmitting a measurement result by measuring the presence and concentration of pathogens of the test material using the optical characteristics; And a disease management server for receiving a measurement result transmitted from the portable measuring device and storing the result in a database, and transmitting a warning message according to preset setting information when it is determined that the test substance is infected with a pathogen as a result of analyzing the received measurement result. Provided is a disease diagnosis / management system using a portable measuring device comprising a.

Here, the above-described alert message may be configured to include at least one of a message transmitted to the administrator's mobile communication terminal through the mobile communication network, and a message transmitted to the administrator terminal through the network.

In addition, the above-described portable measuring device stores the unique identification information, the measurement result transmitted from the portable measuring device includes the unique identification information, the disease management server is a portable measuring device included in the received measurement results It may be configured to determine at least one or more information of the local information, affiliated organization information, the measurer information of the measuring device using the unique identification information of the.

In addition, the disease diagnosis / management system using the above-mentioned portable measuring device, if the alarm message is transmitted from the disease management server, receiving and storing it, and further comprising a related agency server for propagating the alarm message according to a preset procedure; It is preferred to be configured.

On the other hand, according to a preferred embodiment of the present invention, the immuno-aggregated scattered light and PDA fluorescence biochip measurement device may be adopted as the above-described portable measurement device, wherein the immuno-aggregated scattered light and PDA fluorescence biochip measurement device is equipped with a measurement sample A chip loading unit; A light source unit including at least one light source for supplying measurement light; The spectacle is connected to any one of the light sources included in the light source unit according to the operator's operation, and supplies the light for measurement supplied from the connected light source to the measurement sample, receives the light passing through the measurement sample, and supplies the light to the detection unit. Optical path means for forming a furnace; And a detector for detecting light transmitted through the optical path means.

Here, the above-described light source unit, the first light source for supplying light for measuring immuno-aggregated scattered light;

A second light source for supplying light for PDA embolism reaction measurement; A first ferrule installed above the first light source to which the optical path means is connected / fixed; And a second ferrule installed above the second light source to which the light path means is connected / fixed.

More preferably, the above-described light source unit is provided between the second light source and the second ferrule, and bypasses only the light of a specific wavelength band of light supplied from the second light source to supply the filter slider to the optical path means. It may further include.

In addition, the above-described optical path means, the first optical fiber is connected to any one of the light source in accordance with the operator's operation to supply the light for the measurement sample mounted on the chip loading unit; And an optical path unit for supplying the light passing through the measurement sample mounted to the chip loading unit to the detection unit.

More preferably, the above-described light path unit, a ferol is attached to one side of the chip loading portion to receive the light passing through the measurement sample to supply to the connected optical fiber; And a second optical fiber supplying light supplied to the ferrol to the detection unit.

Here, the above-described ferrule is configured to be detachably attached to one side of the chip loading portion, and when attached to one side of the chip loading portion according to the selection of the measuring device, the inclined ferrule and the scattering light passing through the measurement sample, and the chip loading portion It is detachably configured on one side and is attached to one side of the chip loading part according to the selection of the measuring device among the upright ferrules in which light passing through the measurement sample is received when attached to one side of the chip loading part according to the selection of the measuring person. Can be.

On the other hand, the above-described detection unit, the detection unit ferrule is fixed to the optical fiber of the optical path means;

A condensing lens installed at one side of the detection unit ferrule and condensing light transmitted through the optical fiber; A filter wheel for passing only a specific wavelength of light transmitted through the condenser lens; And an optical diode which receives the light transmitted through the filter and converts the light into a current signal.

In this case, the above-described filter wheel may include at least one filter, and may be configured to be operated to position a specific filter between the condenser lens and the photodiode according to a measurer's manipulation.

In addition, the filter wheel described above, the transmission filter for measuring the immuno-aggregated scattered light; And a bandpass filter for measuring a PDA embolism response.

On the other hand, according to another preferred embodiment of the present invention, the immuno-aggregated scattered light and PDA fluorescence biochip measuring device using a spectrometer may be adopted as the above-described portable measuring device, immuno-aggregated scattered light and PDA fluorescent biochip measuring device using the spectrometer The chip loading unit is mounted to the measurement sample; A light source unit including at least one light source for supplying measurement light; A first optical fiber connected to the light source unit to irradiate light supplied from the light source unit with a measurement sample mounted to the chip loading unit; A second optical fiber receiving light passing through the measurement sample and supplying the light to a spectroscope; A spectrometer that separates the light supplied from the second optical fiber for each wavelength and detects and outputs light of a specific band; An electrical signal conversion unit for converting the optical signal input from the spectroscope into an electrical signal and outputting the light detected by the spectroscope; And a controller configured to determine pathogens and contamination levels of the measurement sample based on wavelength information of light of a specific region obtained by analyzing the electrical signal.

At this time, the above-described spectroscope, the spectroscope body formed with a light inlet to which the optical fiber is fixed on one side; A light collecting slit installed inside the body of the spectroscope and collecting light introduced into the light inlet into one place; A first reflecting mirror installed inside the spectrometer body and reflecting light transmitted from the condensing slit; A second reflector installed inside the spectrometer body and reflecting the light transmitted from the first reflector by wavelength band; A third reflector installed inside the spectrometer body and reflecting light transmitted from the second reflector; It may include a detector installed inside the spectrometer body to detect the light transmitted from the third reflecting mirror.

The light source unit may include a light source for measuring immunoaggregation scattering light and a light source for measuring PDA embolization reaction, and the first optical fiber may react with the light source for immunoaggregation scattering light measurement and PDA embolization according to an operator's manipulation. It may be configured to be connected to any one of the light source for the measurement.

On the other hand, according to another preferred embodiment of the present invention, as the portable measuring device described above, the portable measuring device may be a particle immune coagulation reaction scattered light measuring device, the above-described particle immune coagulation reaction scattered light measuring device, A light source unit to be irradiated; A microfluidic chip that scatters the irradiated light at a point where aggregation reaction between a test substance and antibody stator particles occurs; An optical fiber for transmitting scattered light scattered from the microfluidic chip and a CCD array sensor for converting the transmitted scattered light into an electrical signal; Communication unit for transmitting the characteristic information of the test substance and the concentration information according to the intensity of the scattered light by a specific communication method; And acquiring wavelength information of light of a specific region by analyzing the electrical signal, confirming pathogens and contamination levels of the test material based on the wavelength information of the light, and analyzing concentrations according to the intensity of the scattered light. It may include a microprocessor for controlling the transmission of pathogens, contamination levels and analyzed concentration information based on the specific communication method.

Here, the above-mentioned wavelength information of light is preferably a wavelength of 380 nm.

In addition, the above-described microprocessor, the converter converts the analog electrical signal into a digital signal and outputs; And a memory storing a standard characteristic curve of the particle immune coagulation reaction, the concentration characteristic corresponding to the standard intensity and intensity of the scattered light in a standard state in which no pathogen is present for the test substance, and converted into the digital signal. Compare the intensity corresponding to the wavelength information of the obtained light with the standard intensity, check the pathogen and contamination of the test material according to the comparison result, and analyze the concentration according to the intensity of the scattered light based on the standard characteristic curve It can be configured to.

According to one embodiment of the present invention as described above, there is an advantage that it is convenient and simple to measure disease infection using a portable measuring device capable of measuring immunocoagulation scattering light and / or PDA embolism.

In addition, according to a preferred embodiment of the present invention, by using the measurement information measured by using a portable measuring device can be centrally diagnosed and managed for the disease of the national or certain regional unit, and during the initial infection There is an advantage in that it is possible to cope with appropriate infectious diseases.

1 is a block diagram of a disease diagnosis / management system using a portable measuring device according to an embodiment of the present invention,
Figure 2 is a perspective view of the immuno-aggregated scattered light and PDA fluorescence biochip measurement apparatus according to an embodiment of the present invention,
3 is a side view of the immuno-aggregated scattered light and PDA fluorescent biochip measurement apparatus shown in FIG.
4 is a side conceptual view showing a state in which the components of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 2 are connected by optical fibers;
Figure 5 is a flow chart of the immuno-aggregated scattered light and PDA fluorescent biochip measurement device shown in Figure 2,
6 is a state diagram using the immuno-aggregated scattered light and PDA fluorescent biochip measurement device shown in FIG.
FIG. 7 is a conceptual diagram illustrating a light migration path using scattered light in a state diagram of use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 6;
FIG. 8 is a conceptual diagram illustrating a path of light for PDA embolism measurement in a state diagram of use of the immuno-aggregated scattering light and the PDA fluorescence biochip measuring apparatus shown in FIG. 6;
9 is a perspective view showing an immuno-aggregated scattered light and a PDA fluorescence biochip measuring apparatus using a spectroscope according to another embodiment of the present invention,
FIG. 10 is a side view illustrating a spectrometer employed in an immunoaggregated scattered light and a PDA fluorescent biochip measuring apparatus using the spectrometer shown in FIG. 9; FIG.
11 is a perspective view showing an immuno-aggregated scattered light and a PDA fluorescent biochip measuring device according to another embodiment of the present invention,
12 is a side conceptual view showing a state in which the components of an embodiment using a single diode of the immuno-aggregated scattering light and the PDA fluorescent biochip measuring apparatus shown in FIG. 11 are connected by optical fibers;
FIG. 13 is a flow chart of an immunocoagulated scattering light and a PDA fluorescent biochip measuring apparatus shown in FIG. 12;
FIG. 14 is a conceptual diagram illustrating a light migration path using scattered light in a state diagram of use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 12; FIG.
FIG. 15 is a conceptual diagram illustrating a movement path of light for PDA embolism measurement in a state diagram of use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 12;
FIG. 16 is a side conceptual view illustrating a state in which components of an embodiment using a spectrometer are connected with optical fibers among the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 11;
FIG. 17 is a flow chart of an immunocoagulated scattering light and a PDA fluorescent biochip measuring apparatus shown in FIG. 16;
FIG. 18 is a conceptual diagram illustrating a light path using scattered light in a state diagram of use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 16;
19 is a conceptual diagram showing a path of light for PDA embolism measurement in the state diagram of use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 16;
FIG. 20 is a block diagram illustrating an apparatus for measuring scattering light of particle immune agglomeration according to another embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings will be described in detail a preferred embodiment of the disease diagnosis / management system using a portable measuring device according to the present invention.

1 is a block diagram of a disease diagnosis / management system using a portable measuring device according to an embodiment of the present invention. As shown in Figure 1, the disease diagnosis / management system according to the present invention is a portable measuring device (1, 100, 200, 300), diagnostic terminal 400, network 410, disease management server 420, related It may include an institution server 430.

The portable measuring device (1, 100, 200, 300) performs the function of generating and transmitting the measurement result by measuring the presence and concentration of the pathogen of the test material using the optical characteristics. That is, the portable measuring device (1, 100, 200, 300) according to the present invention is a reaction part of the measurement sample that contains a reaction material that can react with the detection material, such as test substances and pathogens contained in the test material It is configured to perform the function of measuring the presence and concentration of pathogens of the test material by irradiating the light for measurement and receiving and analyzing the light passing through the measurement sample. The portable measuring device according to the present invention is an immuno-aggregated scattering light and PDA fluorescent biochip measuring device (1) using a single diode, which will be described later, immuno-aggregated scattering light and a PDA fluorescent biochip measuring device (100) using a spectrometer, immunoaggregation using a light source module The scattered light and PDA fluorescent biochip measuring device 200, the particle immune coagulation reaction scattering light measuring device 300, at least one or more measuring devices may be adopted and used as necessary.

More preferably, the portable measuring device according to the present invention may be configured to perform scattered light measurement and / or PDA (polydiacetylene, polydiacetylene) embolization reaction measurement according to the immunoaggregation reaction.

The measurement of scattered light according to the immunoaggregation reaction can be carried out by irradiating the measuring light to the reaction part where the immune coagulation reaction occurs, and by measuring the scattered light scattered through the reaction part. Means a measurement method. Agglutination is an antigen-antibody reaction, in which particles refer to aggregation, which is widely used for immunological studies, as well as for serological diagnosis, determination of blood type, determination of the fit of blood transfusion, and identification of bacteria in microbiology. It is becoming. On the other hand, an aggregate refers to a state in which particles are stuck to each other after collision and have increased in volume. Therefore, the state of scattered light (intensity of scattered light, etc.) that is scattered when the measurement light is irradiated depends on the presence or absence of the aggregate and the degree of aggregation (the concentration of the aggregate). It is configured to measure the presence and concentration of pathogens in the measurement sample using.

The portable measuring device according to the present invention can be configured to use a microfluidic chip for measuring such an immunoaggregation reaction. The microfluidic chip includes a view point of a channel for introducing a test substance, a channel for inputting antibody-immobilized nanoparticles, and a site where two channels are combined to generate an aggregation reaction by an antigen-antibody reaction. The measuring device according to the present invention is configured to calculate the presence or contamination of pathogens by irradiating measurement light to the viewpoint and measuring / analyzing scattered light generated from the viewpoint.

On the other hand, the measurement of PDA (polydiacetylene) embolism reaction refers to the red color (absorption wavelength) when the stimulus is transmitted to the PDA structure while accessing or binding chemicals or biological materials (antibodies, proteins, peptides, DNA), etc. It is a measurement method using the characteristic of polydiacetylene which causes an embolism reaction toward 550 nm band increase). In other words, the portable measuring device according to the present invention is a specific material (pathogen, protein, virus, etc.) applying the characteristics of polydiacetylene which causes an embolism reaction from blue (600 nm) to red (550 nm) when binding or reaction occurs. PDA biochip for the detection of the test material can be configured to measure the presence and concentration of pathogen infection.

Scattered light measurement and / or PDA (polydiacetylene, polydiacetylene) embolism response measurement according to the immunoaggregation reaction as described above, can be measured only for one type according to the configuration of the portable measuring device according to an embodiment of the present invention In some cases, or by using a single portable measuring device may be configured to allow for the selective measurement of the two types. The portable measuring device stores measurement / diagnostic algorithms and diagnostic information suitable for the measurement method that can be measured and used for measurement and diagnosis.

Detailed configuration and function of the portable measuring device as described above will be described later with reference to FIGS. 2 to 20.

When the portable measuring device according to the present invention finishes the measurement of the measurement sample through the above-described process, it generates and outputs a measurement result (including at least one or more information of pathogen infection, contamination, and concentration). More preferably, when the portable measuring device itself is provided with a display means such as an LED, LCD, etc. capable of outputting the information, the portable measuring device outputs the measured information through the display means to provide the measurer.

In addition, the portable measuring device according to the present invention is preferably provided with a communication means capable of communicating with an external device (diagnostic terminal, disease management server, etc.). According to the present invention, the portable measuring device may be simply configured to be connected to a computer such as a diagnostic terminal 400 through a wired / wireless interface (USB, RS 232, Zigbee, Bluetooth, etc.) to perform data communication. Alternatively, it may be configured to include a communication unit that can perform data communication with the disease management server 420 independently through a wired / wireless network 410, that is, a wired / wireless internet network. When the portable measuring device is configured to have only a communication interface that can be connected only to a computer such as the diagnostic terminal 400, the measurement result output from the portable measuring device to the diagnostic terminal 400 is transmitted through the communication unit of the diagnostic terminal 400. It is configured to be transmitted to the disease management server 420 connected to / wireless network.

More preferably, the unique measuring information may be assigned to the portable measuring device according to the present invention. This is unique information for identifying a plurality of portable measuring devices constituting the disease diagnosis / management system according to the present invention. The database 422 of the disease management server 420 to be described later may be configured according to unique identification information of each portable measuring device. The measuring device information (local information of the portable measuring device, organization information, measuring information, etc.) is structured and stored, and the portable measuring device transmits the measurement result to the disease management server 420, the unique identification information assigned to itself. And the disease management server 420 transmits information corresponding to the unique identification information among the identification information stored in the database 422 using the unique identification information of the portable measuring device included in the received measurement result. Can be configured to be used for disease diagnosis / management procedures. The unique identification information for each portable measuring device may be a product serial number of the portable measuring device or IP information when the fixed IP is configured to be assigned.

On the other hand, the disease diagnosis / management system according to the present invention is more preferably configured to upgrade the measurement / diagnostic algorithm and diagnostic information stored in the portable measuring device using the communication function of the portable measuring device. Such measurement / diagnostic algorithms and diagnostic information can be upgraded automatically or manually. That is, in an embodiment configured to perform an automatic upgrade, when the portable measuring device is connected to the diagnostic terminal 400 or the disease management server 420, the diagnostic terminal 400 or disease management server 420 is connected to the portable measuring device. Check the version information of the stored measurement and diagnostic algorithm and diagnostic information, and if not the latest version is configured to automatically update the stored measurement and diagnostic algorithm and diagnostic information. On the other hand, in the embodiment in which the manual upgrade is performed, the upgrade of the measurement / diagnostic algorithm and the diagnostic information is performed only when there is an upgrade request according to the operator's operation.

On the other hand, the portable measuring device according to the present invention may further include a GPS module (not shown) that can receive the satellite signal transmitted from the GPS satellite to calculate / output the current location information. When the portable measuring device is configured to calculate the current position in this manner, the measurement result using the portable measuring device may be generated / stored including the location information, and the measurement result including the location information may be included in the diagnostic terminal ( 400 or the disease management server 420 has the advantage that can be transmitted. In particular, such location information may be very useful when dealing with an infectious disease.

The diagnostic terminal 400 may be implemented using a personal information communication device such as a desktop, a notebook, and the like, connected to a portable measuring device through a wired / wireless interface (USB, RS 232, Zigbee, Bluetooth, etc.). It receives / stores the measurement result transmitted from the display and outputs it through the display unit. In addition, the diagnostic terminal 400 transmits the received measurement result to the disease management server 420 through a wired / wireless network. On the other hand, as described above, if the portable measuring device itself is configured to perform data communication directly with the disease management server 420 directly through a wired / wireless network independently diagnostic terminal 400 according to the present invention May be omitted in diagnostic / management systems.

Meanwhile, according to the present invention, the portable measuring device as described above may be configured to simply measure the optical characteristics of the measuring sample and transmit the measured result to the connected diagnostic terminal 400. When configured in this manner, the diagnosis of pathogen infection, pathogen concentration, etc. of the measurement sample by using the measured optical properties will have to be performed in the diagnostic terminal 400.

The disease management server 420 receives the measurement result transmitted through the portable measuring device 1, 100, 200, 300 or the diagnostic terminal 400 connected through the wired / wireless network 410 and stores it in a database. In the database 422 provided in the disease management server 420, measurement device information (local information, organization information, measurer information, etc.) according to unique identification information of each portable measurement device (1, 100, 200, 300) is structured. And stored, and the measurement results transmitted from each measuring device itself or via the diagnostic terminal 400 are structured and stored.

In addition, the disease management server 420 according to the present invention, when it is determined that the test material is infected with the pathogen as a result of analyzing the received measurement results, that is, if it is determined that the disease has occurred an alarm message according to the preset setting information Will be sent. That is, the disease management server 420 according to the present invention may be configured to transmit an alarm message to at least one or more administrator terminals (not shown) connected through an internal network or an external network when it is determined that a disease has occurred. In addition, it may be more preferably configured to transmit an alert message consisting of a text message or a multimedia message to the manager's mobile communication terminal through the mobile communication network using the registered manager's mobile communication terminal information.

On the other hand, the disease management server 420 according to the present invention is from a terminal (terminal controlled by a person allowed to access the server, such as administrator terminal, diagnostic terminal, general terminal) connected to the server via a wired / wireless network It may be configured to receive the transmitted information providing request, to read / process the measurement result stored in the database 422 according to the received information providing request and to transmit the information to the requesting terminal. In this case, at least one or more measurement results read from the database 422 may be configured to be generated as statistical information and provided to the terminal through statistical processing according to the type of information providing request.

Meanwhile, more preferably, the disease diagnosis / management system according to the present invention receives and stores an alarm message when the alarm message is transmitted from the disease management server 420 and propagates the alarm message according to a preset procedure. It may be configured to further include. Such related agencies may include various government agencies and public organizations that carry out relevant tasks such as disease / quarantine / health. The related agency server 430 sends an alarm message to a related agency manager terminal (not shown) and a related agency manager mobile communication terminal (not shown) according to a preset processing procedure when an alarm message is transmitted from the disease management server 420. It is even more desirable to be configured to retransmit.

FIG. 2 is a perspective view illustrating an immunoaggregated scattered light and a PDA fluorescent biochip measuring apparatus according to an embodiment of the present invention, FIG. 3 is a side view of the immunoaggregated scattered light and the PDA fluorescent biochip measuring apparatus illustrated in FIG. 2, and FIG. 2 is a side conceptual view showing a state in which the components of the immuno-aggregated scattered light and PDA fluorescent biochip measuring apparatus are connected by optical fibers, and FIG. 5 is a flow chart of the immuno-aggregated scattering light and PDA fluorescent biochip measuring apparatus shown in FIG. FIG. 6 is a diagram illustrating the use of the immunoaggregated scattered light and the PDA fluorescence biochip measuring apparatus illustrated in FIG. 2, and FIG. 7 is a diagram illustrating a movement path of the light using the scattered light in the state diagram of the immunoaggregated scattered light and the PDA fluorescent biochip measuring apparatus illustrated in FIG. 6. 8 is a conceptual diagram of the PDA embolization side in the state diagram of the use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. It is a conceptual diagram showing a travel path of light for.

According to a preferred embodiment of the present invention, immuno-aggregated scattered light and PDA fluorescence biochip measuring device may be adopted as a portable measuring device constituting a disease diagnosis / management system using the portable measuring device as described above.

As shown in the accompanying drawings, the immuno-aggregated scattered light and PDA fluorescent biochip measuring device 1 (first embodiment) includes a main body 10, a light source unit 20, a filter slider 30, a light The route unit 40 may include a detector 50, a controller 60, and a display 80.

As shown in FIG. 1, the main body 10 is preferably formed in a rectangular parallelepiped shape and small in size to facilitate portability. On the upper side of the main body 10 is provided a chip loading section 11 on which a measurement sample is mounted. The chip loading unit 11 may employ a microfluidic chip 12 for measuring an immunoaggregation reaction or a PDA biochip (not shown) for measuring an PDA embolism reaction. The microfluidic chip 12 has a channel through which the test substance and the antibody stator particles can be introduced, and the microfluidic chip 12 is transferred from the light source unit 20 to be described later to a portion where the aggregate is generated by the agglomeration reaction between the test substance and the antibody stator particles. Incoming light is irradiated.

As shown in FIG. 3, the light source 20 is installed inside the main body 10 and supplies light irradiated to the microfluidic chip 12 or the PDA biochip mounted on the chip loading unit 11. 3, the first and second light sources 21 and 22 installed on one side of the main body 10 and the first and second light sources 21 and 22 installed on the inner side of the main body 10, and the chip 20. Ferrule 23 for fixing the first optical fiber 70 connected to the loading unit 11 (installed on the upper side of the first light source and above the first ferrol and the second light source to which the first optical fiber 70 is connected / fixed) And a second ferrol to which the first optical fiber 70 is connected / fixed.

Here, the first optical fiber 70 is connected / fixed to the first ferrol or the second ferrol installed above the first light source 21 or the second ferrol installed above the first light source 21 according to the operator's manipulation. Light supplied from the first light source 21 or the second light source 22 is supplied to the measurement sample.

Preferably, the first light source 21 uses an ultraviolet LED (UV LED), and the second light source 22 preferably uses a xenon lamp (or halogen lamp). The xenon lamp can generate a broad spectrum of light from ultraviolet to infrared with high efficiency, and has an output of about 30 to 50W, and its efficiency is very high, which is advantageous in converting 50% of electrical energy into light. have.

The first light source 21 is used as a light source for measuring scattered light, and preferably has a wavelength of 380 nm, and the second light source 22 is used as a light source for detecting a liposome (Liposome) liposome in a PDA (polydiacetylene). It is used and preferably has a wavelength of 220 ~ 720nm.

The above-described polydiacetylene (PDA) is one of conjugated polymers having a change in conductivity, redox potential, absorption or emission spectrum according to the change of environment. Polydiacetylene is a polymerization form of amphiphilic monomers, and diacetylene, which is a monomer, has an advantage of easily self-assembling into a three-dimensional spherical structure in an aqueous solution. The three-dimensional spherical structure thus formed is polymerized between the monomers and the triple bonds adjacent to each other at 254 nm, and the colorless aqueous solution is changed into the blue aqueous solution having the maximum absorption wavelength at 650 nm. Blue polydiacetylene has a characteristic that the color changes according to external environmental factors. For example, when the stimulus is transferred to the PDA structure with access or binding of temperature, pH, chemicals or biomaterials (antibodies, proteins, peptides, DNA), the embolism shifts toward red (increased absorption wavelength 550nm band). Will be done. According to the degree of such external factors, the color transition appears differently, and the fluorescence characteristic that did not appear in blue has the characteristic that the maximum emission energy is stronger in the 630nm portion as the color transition occurs. Examples of sensors that can be recognized through embolization of PDA liposomes include antibody response sensors, peptide and protein reaction sensors, sensors that detect through film form or immobilization on a support, and sensors that use electrical signal conversion. That is, as described above, the antigen-antibody reaction, the peptide reaction, the protein reaction, and the like, by detecting the PDA liposome embolism, the reaction and the degree of the reaction can be measured. PDA biochip refers to a biosensor configured to detect a substance to be measured using the PDA liposome embolism reaction. For example, the PDA biochip for the detection of influenza virus can be used for influenza virus detection by binding the antibody to the ends of the liposomes that have already been made so that the color change for the selective and specific reaction with the antigen can be made.

As described above, the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus according to the present invention can be used to selectively use two light sources (ultraviolet LED (UV LED), xenon lamp) appropriately according to the measurement / analysis target Composed,

In the case of selecting the first light source 21, by measuring the scattered light according to the immunoaggregation reaction, it is possible to detect the presence or absence of contamination of the pathogen of the measurement sample,

When the second light source 22 is selected, a PDA (polydiacetylene, polydiacetylene) liposom (Liposom) by sensing the metabolic reaction is configured to diagnose the presence or absence of pathogens in the measurement sample.

On the other hand, immuno-aggregated scattered light and PDA fluorescence biochip measuring device according to the present invention is to measure the wavelength and the measurement sample of the light supplied to the measurement sample in order to more accurately and efficiently sense the PDA liposome embolism reaction using the second light source as described above It is more preferable to further include the structure which can limit the wavelength of the light transmitted and received by the photodiode 54.

That is, the filter slider 30 for limiting the wavelength of the light supplied to the measurement sample to a single wavelength is preferably installed above the second light source 22 as shown in FIG. 4 and as shown in FIG. 5. Filtering the light emitted from the second light source 22 to a specific wavelength, a slider body (not shown) provided on one side of the main body 10, and a filter that is preferably fitted to the hole formed in the slider body ( Not shown). The filter is configured as a 600 nm bypass filter, preferably configured to pass only light having a wavelength of 600 nm (blue wavelength), which is responsible for the PDA liposome embolism reaction (ie, red embolism reaction). In sensing, light emitted to a measurement sample as a light source is formed into a single wavelength of light (ie, light of blue wavelength) to improve accuracy and efficiency in sensing an embolism reaction.

As shown in FIG. 4, the optical path unit 40 is a light path guiding the light to be transmitted to or scattered by the aggregate to be transmitted to the detection unit 50, which will be described later. 11 is installed on the upper side of the inclined ferrule 41 and the chip loading portion 11 is mainly installed in the upper side of the first light source 21 is transmitted from the first light source (21) to be transmitted to the detection unit 50. The upright ferrule 42 for transmitting the light emitted from the second light source 22 and passing through the PDA biochip to the detection unit 50, and the light flowing into the inclined ferrule 41 and the upright ferrule 42 is detected ( The second optical fiber 43 is connected to the 50 to be delivered.

The detection unit 50 measures the amount of light transmitted from the optical path unit 40 and transmits it to the control unit 60 to be described later. As shown in FIG. 3, the detection unit 50 is installed at one side of the main body 10 and the optical path is provided. The detection unit ferrule 51 to which the second optical fiber 43 of the unit 40 is fixed, and the light collecting lens 52 which is installed above the detection unit ferrule 51 and collects the light flowing through the detection unit ferrule 51. And a filter wheel assembly 53 installed at the main body 10 so as to be positioned below the detection unit ferrule 51 and transmitting the light transmitted through the condenser lens 52 entirely or only a required wavelength. It is installed in the main body 10 to be located below the filter wheel assembly 53 and consists of a photodiode 54 for converting the light transmitted through the filter wheel assembly 53 into a photocurrent to be transmitted to the control unit 60. .

The filter wheel assembly 53 is composed of a filter wheel 53a which is installed to be slidable to the main body 10 and a filter 53b which is preferably fitted to two filter wheels 53a. One of the two filters 53b preferably uses a transmission filter to transmit scattered light, and the other filter uses a bandpass filter to transmit 550 nm wavelength for PDA. As described above, the color transfer of PDA liposomes occurs when the reaction (combination) occurs from blue (absorption wavelength 600nm) to red (absorption wavelength 550nm), so the filter for sensing the metastasis reaction of the PDA liposome 550nm When the bypass filter is adopted, only the single wavelength light (550 nm red), which is a measurement target for measuring the metabolic reaction, is received by the photodiode 54 so that the light-receiving unit is arranged in the CCD array. Instead of a (CCD array) it can be configured with only a single photodiode 54, there is an advantage that can simplify the device and analysis algorithm, reduce the portability and production cost of the device configuration.

Here, the above-described two filters 53b are selected according to a measurement method that an experimenter (measurer) wants to measure so that the optical path unit 40 is connected.

That is, when the measurer wants to measure the immunoaggregation reaction using the scattered light, the first light source 21 is selected as the light source to irradiate light onto the measurement sample through the first optical fiber 70, and the above-described light path One side of the unit 40 is connected to the above-mentioned inclined ferrule 41 and the other side is connected to a transmission filter through which scattered light is transmitted, and transmits scattered light scattered from the measurement sample to the photodiode 54. Will be done.

On the other hand, when the measurer wants to measure the PDA liposome embolism reaction, the second light source 22 is selected as the light source and irradiates 600 nm wavelength light to the measurement sample through the filter slider 30 and the first optical fiber 70. One side of the above-described optical path unit 40 is connected to the above-described upright ferrule 42, and the other side is connected to a bandpass filter to transmit a 550 nm wavelength of the two filters 53b. Light of 550 nm wavelength emitted from the sample will be delivered to the photodiode 54.

The controller 60 acquires light quantity information of a specific region by analyzing the electrical signal transmitted from the detection unit 50, and checks pathogens and contamination levels of the test substance based on the obtained light quantity information. The control unit 60 specifically corresponds to a converter (not shown) for converting an analog electric signal into a digital signal, and a standard intensity and intensity for scattered light in a standard state in which a test substance is not contaminated for measuring immuno-aggregated scattered light. PDA consisting of standard characteristics curve for immunoaggregation reaction and concentration information corresponding to standard intensity and intensity for light of 550nm wavelength from PDA biochip in standard state without reaction for PDA embolism measurement It includes a memory (not shown) that stores a standard characteristic curve for the embolic response.

The control unit 60 compares the intensity and the standard intensity corresponding to the light quantity information obtained by converting the digital signal according to the measurement mode selected by the measurer, confirms the pathogen and contamination level of the test substance according to the comparison result, and sets the standard characteristic curve. Based on the intensity of the light is analyzed.

In more detail, when the measurer selects the immunoaggregated scattering light measurement, the controller 60 compares the light intensity information obtained from the detector 50 with the standard characteristic curve for the immunoaggregation reaction stored in the memory to determine the pathogen of the measurement sample. The presence and pollution level are calculated. On the other hand, when the measurer selects the PDA embolism response measurement, the control unit 60 compares the light intensity information obtained from the detection unit 50 with the standard characteristic curve for the PDA embolism reaction stored in the memory to determine the presence of pathogens and contamination levels in the measurement sample. Will yield.

The display unit 80 displays specific information transmitted from the control unit 60 on the screen. Preferably, display means such as an LCD may be adopted, and may be replaced with other known display means.

FIG. 6 is a diagram illustrating the use of the immunoaggregated scattered light and the PDA fluorescence biochip measuring apparatus shown in FIG. 2, and FIG. 7 is a diagram illustrating the movement path of the light using the scattered light in the state diagram of the immunoaggregated scattered light and the PDA fluorescent biochip measuring apparatus illustrated in FIG. 6. FIG. 8 is a conceptual diagram illustrating a movement path of light for PDA embolism measurement in a state diagram of use of the immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus illustrated in FIG. 6.

Hereinafter, the immunoaggregated scattering light measurement process of the immunoaggregated scattered light and the PDA fluorescence biochip measuring device 1 according to an exemplary embodiment of the present invention will be described with reference to FIGS. 6 to 7.

As described above, the first light source 21 (380 nm ultraviolet LED (UV LED)) is selected as the light source when measuring the immuno-aggregated scattering light, and the first optical fiber 70 is connected to one side of the optical path unit 40. Is connected to the aforementioned inclined ferrule 41 and the other side is connected to a transmission filter through which scattered light is transmitted among the two filters 53b, thereby forming an optical path for immunoaggregated scattered light measurement.

On the other hand, in order to detect the immunoaggregation reaction, the test substance and the antibody stator particles are introduced into each of the channels of the microfluidic chip 12, and the microfluidic chip 12 is mounted in the chip loading part 11.

When the measurement is started in this state, the light irradiated from the first light source 21 passes through the first transmission irradiation lens L, is supplied to the first optical fiber 70, and then the second transmission irradiation lens L is again provided. It passes through and irradiates to the microfluidic chip 12 mounted in the chip loading part 11.

When the light is irradiated from the first light source 21, the microfluidic chip 12 generates scattered light, and the generated scattered light is supplied to the second optical fiber 43 connected through the inclined ferrule 41 to condense the incoming light. The light is collected by the condenser lens 52. Scattered light collected through the condenser lens 52 is supplied to the photodiode 54 through a transmission filter for transmission.

The photodiode 54 converts the received scattered light into a current signal and outputs it to the controller 60. The controller 60 converts the input analog current into digital data to obtain light quantity information of the digitized scattered light. By comparing the obtained light quantity information and the standard characteristic curve for the immunoaggregation reaction stored in the memory, the presence or absence of contamination of the measurement sample is calculated, and the calculated information is stored in the memory and / or output through the display unit 80.

Next, the PDA embolism measurement process of the immunoaggregated scattered light and the PDA fluorescence biochip measuring apparatus 1 according to a preferred embodiment of the present invention will be described with reference to FIGS. 6 and 8.

As described above, the second light source 22 (xenon lamp: 220-720 nm) is selected as the light source when measuring the PDA embolism reaction, and the first optical fiber 70 is connected to the optical path unit 40. One side is connected to the above-described upright ferrule 42, and the other side is connected to a band pass filter (550 nm bandpass filter) so that only light of 550 nm wavelength of the two filters (53b) is transmitted, the sight for measuring PDA embolism response The furnace is constructed.

On the other hand, in order to detect the PDA embolism reaction, a test substance is added to the PDA biochip, and the PDA biochip is mounted on the chip loading unit 11.

When the measurement starts in this state, the light irradiated from the second light source 22 passes through the filter slider 30 (600 nm bandpass filter), and only light of a single wavelength (600 nm) is bypassed, and the bypassed single wavelength ( 600 nm) light is passed through the first transmission lens (L) and supplied to the first optical fiber 70, and then passes through the second transmission irradiation lens (L) to the PDA biochip mounted on the chip loading section (11). Is investigated.

The light passing through the PDA biochip is condensed by the condenser lens 52 that condenses the light that is supplied to the second optical fiber 43 connected through the upright ferrule 42. The light collected through the condenser lens 52 passes through a 550 nm bandpass filter, and only light having a 550 nm wavelength is bypassed, and light of the bypassed 550 nm wavelength is supplied to the photodiode 54.

The photodiode 54 converts the received light of 550nm wavelength into a current signal and outputs it to the controller 60, and the controller 60 converts the input analog current into digital data to obtain light quantity information of the digitized 550nm wavelength light. Then, by comparing the obtained light quantity information and the standard characteristic curve for the PDA embolism reaction stored in the memory to calculate the presence of pathogens and contamination of the measurement sample, the calculated information is stored in the memory and / or via the display unit 80 Will print.

FIG. 9 is a perspective view illustrating an immunoaggregated scattered light and a PDA fluorescence biochip measuring apparatus using a spectrometer according to another embodiment of the present invention, and FIG. 10 is employed in an immunoaggregated scattered light and a PDA fluorescent biochip measuring apparatus using the spectrometer shown in FIG. 9. Side view showing the spectrometer.

According to another preferred embodiment of the present invention, as a portable measuring device constituting the disease diagnosis / management system using the portable measuring device as described above, the immuno-aggregated scattered light and the PDA fluorescent biochip measuring device (second embodiment) Can be adopted.

The immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus 100 using the spectrometer according to another embodiment of the present invention is the main body 110, the light source unit 120, the spectrometer 130, the electrical signal conversion unit 140, the controller 150 ) May include a communication unit 160 and a display unit 170.

As shown in FIG. 9, the main body 110 is preferably formed in a rectangular parallelepiped shape and small in size to facilitate portability. On the upper side of the main body 110 is provided a chip loading portion 111 that accommodates the test substance and the antibody stator particles. In one embodiment of the present invention, the test material and the antibody stator particles are preferably installed in the form of a microfluidic chip 112 in the chip loading portion 111. The microfluidic chip 112 has a channel and an observation window into which the test substance and the antibody stator particles can be input, respectively, and the light source unit 120 which will be described later in a part where an aggregate is generated by the agglomeration reaction between the test substance and the antibody stator particles. The light transmitted from) is irradiated.

The light source unit 120 is installed on the main body 110 so as to be positioned below the microfluidic chip 112 as shown in FIG. 9, and irradiates aggregates formed on the microfluidic chip 112 mounted to the chip loading unit 111. As for supplying the light, preferably an ultraviolet LED (UV LED) having a wavelength of 380 nm is used. The ultraviolet LED may be replaced with the above-described xenon lamp preferably having a wavelength of 220 ~ 720nm. As described in the above embodiment, the ultraviolet LED is preferably used as a scattering light source, and the xenon lamp is used as a PDA light source.

The spectrometer 130 is installed inside the main body 110 as shown in FIG. 9 and is connected to the microfluidic chip 112 with the optical fiber 200 to detect light irradiated to the aggregates, thereby detecting an electric signal converter (described later). As transmitted to 140, the spectrometer body 131 and the spectrometer body 131 is installed in the main body 110 and the optical inlet 131a is formed on one side is fixed to the optical fiber 200 as shown in FIG. It is installed inside the light collecting slit 132 to collect the light flowing into the light inlet 131a in one place, and installed inside the spectrometer body 131 and reflects the light transmitted from the light collecting slit 132 The first reflector 133, the second reflector 134 is installed inside the spectrometer body 131, and the light reflected from the first reflector 133 is dispersed and reflected for each wavelength band, and the spectrometer body ( Is installed inside the 131 and reflects the light transmitted from the second reflector 134 The key is installed inside the third reflector 135 and the spectroscope body 131 and detects the intensity by receiving light from the third reflector 135 and is connected to an electrical signal converter 140 to be described later. 136.

The electrical signal conversion unit 140 is installed inside the main body 110 as shown in FIG. 9 and converts light transmitted through the spectrometer 130 into an electrical signal, preferably using a CCD array sensor. .

The controller 150 analyzes the electrical signal transmitted from the electrical signal converter 140 to obtain wavelength information of light in a specific region, and checks pathogens and contamination levels of the test material based on the wavelength information of the light. The control unit 150 specifically includes an converter (not shown) for converting an analog electric signal into a digital signal, and an immunity including concentration information corresponding to standard intensity and intensity for scattered light in a standard state in which the test substance is not immunized. It includes a memory (not shown) that pre-stores the standard characteristic curve of the flocculation reaction, and compares the intensity and standard intensity corresponding to the wavelength information of the light obtained by changing into a digital signal, and according to the comparison result pathogens and Check the contamination level and analyze the concentration according to the intensity of light based on the standard characteristic curve.

The display unit 170 displays specific information transmitted from the control unit 150 on the screen. Preferably, the display unit 170 is installed above the main body 110 and uses an LCD display.

The communication unit 160 is installed inside the main body 110 as shown in FIG. 9, and is connected to a computer or a terminal connected by the control unit 150 to provide concentration analysis information along with confirmed pathogens and contamination levels of the test substance. send. The communication unit 160 preferably uses any one of USB, RS232, or IrDA.

According to a preferred embodiment of the present invention, an immuno-aggregated scattered light and a PDA fluorescent biochip measuring device using a plurality of light source modules may be adopted as the portable measuring device constituting the disease diagnosis / management system using the portable measuring device as described above. .

11 is a perspective view showing an immuno-aggregated scattered light and a PDA fluorescence biochip measuring device according to another embodiment (third embodiment, fourth embodiment) of the present invention.

Another embodiment (third embodiment, fourth embodiment) of the present invention described below is different from the embodiment described with reference to FIGS. 2 to 10 (first embodiment, second embodiment) and two light sources ( The scattering light measuring light source and the PDA color transition measuring light source are not configured to be fixedly provided in the measuring apparatus 200, and two light sources (the scattering light measuring light source and the PDA color transition measuring light source) are separately formed as separate light source modules. The biggest difference is that the light source module selected by the measurer is configured to be used in combination with the measuring device.

Due to this difference, in the above-described first and second embodiments, it is necessary to separately configure an optical path by operating an optical fiber, etc. according to the type of light source selected by the measurer as the light source for measurement. Therefore, since the required light source module of the plurality of light source modules is coupled to the measuring device, there is a difference that the optical path itself composed of an optical fiber or the like is almost fixed.

Hereinafter, a third embodiment and a fourth embodiment of an immunoaggregated scattered light and a PDA fluorescence biochip measuring apparatus 200 according to the present invention will be described based on the differences described above with reference to FIGS. 11 to 19. .

The immuno-aggregated scattered light and the PDA fluorescence biochip measuring apparatus 200 according to the third and fourth embodiments of the present invention include a main body 210, a light source unit 220, a detection unit 230 or 250, and an electrical signal conversion unit ( Not shown), the control unit 260, the communication unit 270, and a display unit 280.

As shown in FIG. 11, the main body 210 is preferably formed in a rectangular parallelepiped shape and small in size to facilitate portability. On the upper side of the main body 210 is provided a chip loading section 211 is mounted a measurement sample. The chip loading unit 211 may employ a microfluidic chip 212 for measuring an immunoaggregation reaction or a PDA biochip (not shown) for measuring a PDA embolism reaction. The microfluidic chip 212 has a channel through which the test substance and the antibody stator particles can be input, respectively, and is transmitted from the light source unit 220 to be described later to a portion where the aggregate is generated by the agglomeration reaction between the test substance and the antibody stator particles. Light is irradiated. PDA biochip refers to a biosensor configured to detect a substance to be measured using a PDA liposome embolism reaction. For example, the PDA biochip for the detection of influenza virus can be used for influenza virus detection by binding the antibody to the ends of the liposomes that have already been made so that the color change for the selective and specific reaction with the antigen can be made.

As shown in FIG. 11, the light source unit 220 is installed at the main body 210 so as to be positioned below the measurement sample and supplies measurement light to the microfluidic chip 212 or the PDA biochip mounted on the chip loading unit 211. Done. As described above, the light source unit according to the third and fourth embodiments of the present invention is configured to combine the light source module selected by the measurer among the plurality of light source modules to supply the light for measurement. More preferably, the light source module used in the present invention is configured to be coupled / fixed to the light source unit according to the present invention, and furthermore, according to the object of the present invention, an ultraviolet LED (UV LED) having a wavelength of 380 nm for measuring scattered light. Any one of the first light source module is configured, the second light source module consisting of a xenon lamp (or halogen lamp) having a wavelength of 220 ~ 720nm for PDA embolism reaction measurement is coupled to the light source unit according to the needs of the measurement Can be configured. It will be apparent to those skilled in the art that the above-described first light source module and second light source module are merely exemplary, and more light source modules and various light source modules may be applied and used.

The detector 230 or 250 detects the light passing through the measurement sample, converts the light into an electrical signal, and outputs the signal to the controller 260. Such a detector according to the present invention may be composed of a single diode detector using a single diode according to the invention (third embodiment), or may be composed of a spectrometer detector using a spectrometer (fourth embodiment). . The detailed configuration of this third embodiment will be described later with reference to FIGS. 12 to 15, and the detailed configuration of the fourth embodiment will be described below with reference to FIGS. 16 to 19.

The controller 260 analyzes the electrical signal transmitted from the detector 230 or 250 to obtain light quantity information of a specific region, and checks pathogens and contamination levels of the test substance based on the obtained light quantity information. The controller 260 specifically corresponds to a converter (not shown) for converting an analog electrical signal into a digital signal, and a standard intensity and intensity for scattered light in a standard state in which a test substance is not contaminated for measuring immunoaggregated scattered light. PDA consisting of standard characteristics curve for immunoaggregation reaction and concentration information corresponding to standard intensity and intensity for light of 550nm wavelength from PDA biochip in standard state without reaction for PDA embolism measurement It includes a memory (not shown) that stores a standard characteristic curve for the embolic response.

The control unit 260 compares the intensity and the standard intensity corresponding to the light quantity information obtained by converting the digital signal according to the measurement mode selected by the measurer, confirms the pathogen and contamination level of the test substance according to the comparison result, and sets the standard characteristic curve. Based on the intensity of the light is analyzed. In more detail, when the measurer selects the immunoaggregated scattered light measurement, the controller 260 compares the light intensity information obtained from the detector with a standard characteristic curve for the immunoaggregation reaction stored in the memory to determine the presence of pathogens and contamination levels in the sample. Will yield. On the other hand, when the measurer selects the PDA embolism response measurement, the controller 260 compares the light quantity information obtained from the detector with the standard characteristic curve for the PDA embolism reaction stored in the memory to calculate the presence of pathogens and the contamination level of the measurement sample. do.

The display unit 280 displays specific information transmitted from the control unit 260 on the screen. Preferably, the display unit 280 is installed above the main body 210 and uses an LCD display.

The communication unit 270 is installed inside the main body 210 as shown in FIG. 10, and is connected to a computer or a terminal connected by the control unit 260 to provide concentration analysis information along with confirmed pathogens and contamination levels of the test substance. send. The communication unit 270 is preferably configured to support at least one or more of USB, RS232, or wired / wireless communication methods that provide an interface by direct connection with the terminal.

FIG. 12 is a side conceptual view illustrating a state in which components of an embodiment using a single diode are connected by optical fibers among the immunoaggregated scattered light and PDA fluorescence biochip measuring apparatus shown in FIG. 11, and FIG. 13 is an immunoaggregated scattered light and PDA fluorescence shown in FIG. FIG. 14 is a flow chart of a biochip measuring apparatus, FIG. 14 is a conceptual diagram showing a path of movement of light using scattered light in a state diagram of use of the immunoaggregated scattering light and PDA fluorescent biochip measuring apparatus shown in FIG. 12, and FIG. 15 is an immunoaggregated scattering light shown in FIG. It is a conceptual diagram showing the movement path of light for the measurement of PDA embolism in the use state diagram of the PDA fluorescence biochip measuring device.

Hereinafter, the detailed configuration and function of the measuring apparatus according to the third embodiment of the present invention will be described with reference to FIGS. 12 to 15.

More preferably, the light source unit 220 according to the third embodiment of the present invention is coupled to the light source unit according to the selection of the measurer to supply the measurement light with the first light source module 221 or the second light source module 222 and the light source module. The coupling part 224 and the light source part ferrule 223 may be included.

As described above, the first light source module 221 may be configured as an ultraviolet LED (UV LED) for the scattered light measurement, the second light source module 222 is a xenon lamp (xenon lamp) or for the PDA embolism measurement It may consist of a halogen lamp. On the other hand, the light source module according to the present invention is configured in a form that can be coupled / fixed to the light source module coupling unit 224 to be described later, for the coupling / fixing method already adopted a well-known technology more detailed description Will be omitted.

The light source module coupling unit 224 is configured such that a specific light source module specified by the selection of the measurer can be combined / separated according to the operation of the measurer. It is supplied to the light path means (226, 240) through.

The light source unit ferrule 223 is installed above the light source module coupling unit 224 to connect / fix the optical path means (first optical fiber 226), and to supply the measurement sample measurement light through the first optical fiber. Will perform.

On the other hand, the measuring device according to the present invention may include the light path means (226, 240). Here, the optical path means is a light path for moving the light used in the measuring apparatus according to the present invention, that is, the light source connected to the light source unit for supplying the measurement light supplied from the light source module selected / coupled according to the operator's operation to the measurement sample It means a path for receiving and passing the light passing through the path and the measurement sample to the detection unit, the optical path means means a variety of means that can form such an optical path. As described above, the third and fourth embodiments according to the present invention are configured to couple one of the light source modules specified among the plurality of light source modules to the light source module coupling unit 224 according to the selection of the measurer. Compared to the second embodiment, the optical path is fixedly formed.

The optical path means may include a first optical fiber 226 for supplying the measurement light to the measurement sample and an optical path unit 240 for receiving the light passing through the measurement sample and supplying the light to the detection unit 230. .

The first optical fiber 226 is output from the light source module (the first light source module 221 or the second light source module 222) coupled to / fixed to the light source unit ferrule 223 and coupled to / fixed to the light source module coupling unit 224. The measurement light is supplied to the measurement sample mounted on the chip loading unit.

The light passing through the measurement sample mounted on the chip path of the optical path unit 240 is supplied to the detector 250. More preferably, the optical path unit 240 according to the present invention may include a ferrol 241 or 242 and the second optical fiber 243.

The ferrule is configured so that one side of the second optical fiber 243 is connected / fixed to supply the light passing through the measurement sample to the second optical fiber, and more preferably, the upright ferrule 242 or the inclined ferrule 241 according to the selection of the measurer. Any one of the ferrules) may be selectively used in combination. That is, the upright ferrule 242 or the inclined ferrule 241 may be detachably configured at one side of one side of the chip loading unit, and may be coupled to or separated from one side of the chip loading unit according to the selection of the measurer. Therefore, the measurer forms an optical path by selecting a suitable ferrule among the upright ferrule 242 or the inclined ferrule 241 according to the type of measurement and coupling the ferrule to one side of the chip loading unit.

When measuring the scattered light scattered at a certain angle it is more preferable that the oblique ferrule 241 is coupled to one side of the chip loading portion to form an optical path, when measuring the PDA embolism reaction, the upright ferrule 242 is chip loading It is more preferable to form an optical path on one side of the part. Therefore, when measuring scattered light, the first light source module 221 and the inclined ferrule 241 form a set and are coupled to the measuring device. When measuring the PDA embolism reaction, the second light source module 222 and the upright ferrule are measured. 242 is coupled to the measuring device in a set.

The second optical fiber 243 is connected / fixed to the inclined ferrule 241 or the upright ferrule 242 described above to transmit the light flowing through the inclined ferrule 241 or the upright ferrule 242 to the detection unit 250. do.

The detector 250 according to the third embodiment of the present invention may be configured using a single diode. The single diode detector 250 configured using the single diode is configured similarly to the detector 50 according to the first embodiment described above.

That is, the detector 250 according to the third embodiment of the present invention may include a detector ferrule 251, a condenser lens 252, a filter wheel assembly 253, and a photodiode 254.

The detection unit ferrule 251 is installed on one side of the main body 210 and is configured to connect / fix the second optical fiber 243 of the optical path unit 240. The condenser lens 252 is installed on the upper side of the detection unit ferrule 251 and performs a function of condensing the light flowing through the detection unit ferrule 51 and outputting the light to the filter wheel assembly 253. The condenser lens 252 is not an essential configuration in embodiments according to the present invention, and may be selectively included for efficient condensing an optical signal.

The filter wheel assembly 253 is installed on the main body 210 so as to be positioned below the detection unit ferrule 251, and the condenser lens 252 (in the embodiment including the condenser lens, the second optical fiber when the condenser lens is not included) The light transmitted through the entire transmission or to perform the function to transmit only the required wavelengths. The filter wheel assembly 253 is an essential component of the single diode detection unit 250 constructed using a single diode according to the present invention, and the single diode itself has a problem in that it cannot selectively detect light of a specific wavelength band. Therefore, it is a component devised to solve this problem.

The filter wheel assembly 253 is composed of a filter wheel (53a) that is installed to be slidable to the main body 210, and a filter (253b) that is preferably fitted to two of the filter wheel (253a). One of the two filters 253b preferably uses a global transmission filter to transmit scattered light, and the other filter uses a bandpass filter to transmit 550 nm wavelength for PDA. As described above, the color transfer of the PDA liposomes occurs when the reaction (combination) occurs from blue (600 nm) to red (550 nm), so the 550 nm bypass filter is used to detect the color transfer reaction of the PDA liposomes. In case of adoption, only the single wavelength of light (550nm red), which is the measurement target for the measurement of embolism reaction, is configured to be received by the photodiode 254. Instead of using a single photodiode 254 can be configured instead of the configuration, there is an advantage that it is possible to simplify the device and analysis algorithm and to reduce the portability and production cost of the device configuration. Therefore, when the first light source module is coupled to the measurement device for measuring the scattered light, the global transmission filter will be selected among the two filters 253b, and the second light source module is coupled to the measurement device for the PDA embolism response measurement. The band pass filter will be selected from the two filters 253b.

The photodiode 254 is installed in the main body 210 to be positioned below the filter wheel assembly 253 and converts light transmitted through the filter wheel assembly 253 into a current to be transmitted to the controller 260.

When measuring the scattered light using the third embodiment configured as described above, as shown in Figure 14, the first light source module 221 is coupled to the light source module coupling unit 224 to output the measurement light The output measurement light is supplied to the measurement sample through the first optical fiber 226, and the light passing through the measurement sample is passed to the filter wheel assembly 253 through the inclined ferrule 241 and the second optical fiber 243. Supplied. In this case, since the scattered light is measured, the entire transmissive filter is selected and set among the two filters 253b included in the filter wheel assembly 253. Therefore, all of the light supplied to the filter wheel assembly 253 is output to the photodiode 254.

On the other hand, when measuring the PDA embolism reaction using the third embodiment configured as described above, as shown in FIG. 15, the second light source module 222 is coupled to the light source module coupling unit 224 The measurement light is output, and the output measurement light is supplied to the measurement sample through the first optical fiber 226, and the light passing through the measurement sample passes through the upright ferrule 242 and the second optical fiber 243 through the filter wheel. Supplied to assembly 253. At this time, the PDA embolism is a case of measuring the response, the bandpass filter of the two filters 253b included in the filter wheel assembly 253 is selected and set. Therefore, light belonging to a specific band among the light supplied to the filter wheel assembly 253 is bypassed and output to the photodiode 254.

FIG. 16 is a side conceptual view illustrating a state in which components of an embodiment using a spectrometer are connected by an optical fiber among the immunoaggregated scattered light and the PDA fluorescent biochip measuring device shown in FIG. 11, and FIG. 17 is an immunoaggregated scattered light and PDA fluorescent biochip shown in FIG. 16. 18 is a conceptual diagram showing a path of movement of light using scattered light in the state diagram of use of the immuno-aggregated scattered light and PDA fluorescence biochip measuring device shown in FIG. 16, and FIG. 19 is an immuno-aggregated scattered light and PDA shown in FIG. It is a conceptual diagram showing the path of light for PDA embolism measurement in the state diagram of use of the fluorescent biochip measuring device.

Hereinafter, the detailed configuration and function of the measuring apparatus according to the fourth embodiment of the present invention will be described with reference to FIGS. 16 to 19.

First, the measuring device according to the third embodiment of the present invention described above with reference to FIGS. 12 to 15 and the measuring device according to the fourth embodiment described below differ only in the configuration of the detection unit, Since the detailed configuration and function are the same, the detailed description of the same configuration will be omitted, and the description will be mainly given on the configuration of the detection unit having the difference.

The measuring apparatus according to the fourth embodiment of the present invention may include a light source unit 220, light path means 226 and 240, and a detector 230.

The light source unit 220 according to the fourth exemplary embodiment of the present invention is coupled to the light source unit according to the selection of the measurer to supply the measurement light to the first light source module 221 or the second light source module 222 and the light source module coupling unit ( 224, a light source unit ferrule 223, and the structure and function of the first light source module 221, the second light source module 222, the light source module coupling unit 224, and the light source unit ferrule 223 described above. Same as the third embodiment.

In addition, according to the fourth embodiment of the present invention, the optical path means 226 and 240 are configured to supply the first optical fiber 226 for supplying the measurement light to the measurement sample and the light passing through the measurement sample to the second optical fiber. It may include a ferrule (upright ferrule 242 or inclined ferrule 241) and the second optical fiber 243 is connected to the ferrol to supply the light received by the detection unit 230.

Meanwhile, the detector 230 according to the fourth embodiment of the present invention may be configured using a spectrometer. The spectroscope detector configured using the spectroscope is configured almost similarly to the detector 130 according to the second embodiment described above.

That is, the detection unit according to the fourth embodiment of the present invention may be configured using the spectrometer 230, and more preferably, may further include a detection unit ferrule 251 and a condenser lens 252.

The detection unit ferrule 251 is installed on one side of the main body 210 and is configured to connect / fix the second optical fiber 243 of the optical path unit 240. The condenser lens 252 is installed on the upper side of the detection unit ferrule 251 and performs a function of condensing the light flowing through the detection unit ferrule 51 and outputting the light to the spectrometer 230. The condenser lens 252 is not an essential configuration in embodiments according to the present invention, and may be selectively included for efficient condensing an optical signal.

The spectrometer 230 is installed inside the main body 210 and receives the light passing through the measurement sample through the second optical fiber 243, and selectively receives only light of a specific band according to a preset pass band setting among the received light. It passes through and transmits to the electric signal converter (not shown). In the case of using the spectrometer 230, only the light of a specific band can be selectively supplied to the controller 260 according to a measurer's operation, so that a filter as in the above-described third embodiment is not required, and a finer setting and The advantage is that measurement is possible. It may also be set to function as an all-pass filter when operating the spectrometer. Since the physical and optical configuration of the spectrometer 230 according to the fourth embodiment is the same as the spectrometer 130 according to the second embodiment of the present invention described with reference to FIG. 9, a detailed description thereof will be omitted.

The electrical signal converter is installed inside the main body 110 and converts light transmitted through the spectrometer 230 into an electrical signal. Preferably, a CCD array sensor may be adopted and used.

When measuring the scattered light using the fourth embodiment configured as described above, as shown in Figure 18, the first light source module 221 is coupled to the light source module coupling unit 224 to output the measurement light The output measurement light is supplied to the measurement sample through the first optical fiber 226, and the light passing through the measurement sample is supplied to the spectrometer 230 through the warp ferrule 241 and the second optical fiber 243. . In this case, since the scattered light is measured, the spectrometer 230 is set to allow the entire band (or 380 nm band pass) to pass.

On the other hand, when measuring the PDA embolism reaction using a fourth embodiment configured as described above, as shown in Figure 19, the second light source module 222 is coupled to the light source module coupling portion 224 The measurement light is output, and the output measurement light is supplied to the measurement sample through the first optical fiber 226, and the light passing through the measurement sample is passed through the upright ferrule 242 and the second optical fiber 243 through a spectrometer ( 230). In this case, since the PDA embolism reaction is measured, the spectrometer 230 is set to separate and output only light of a specific band (550 nm wavelength band pass).

FIG. 20 is a block diagram illustrating an apparatus for measuring scattering light of particle immune agglomeration according to another embodiment of the present invention. Hereinafter, a portable measuring device according to another exemplary embodiment of the present invention will be described with reference to FIG. 20.

As shown in FIG. 20, a particle immunoaggregating reaction scattered light measuring device (a fifth embodiment) may be adopted as a portable measuring device constituting a disease diagnosis / management system according to another exemplary embodiment of the present invention.

First, a schematic operation concept of the particle immune aggregation reaction scattering light measuring apparatus according to the present invention will be described.

The particle immune coagulation reaction scattered light measuring apparatus 300 according to the present invention includes a microfluidic chip 320 and a microprocessor 350 for measuring and analyzing the intensity and concentration of the scattered light incident from the optical fiber connected to the measuring apparatus therefrom. It may include an optical measurement sensor system.

First, the microfluidic chip 320 includes a view point of a site into which a test substance is introduced, a channel into which antibody immobilized nanoparticles are input, and two channels are combined to generate an aggregation reaction by an antigen antibody reaction. In addition, the measuring apparatus according to the present invention is configured to calculate the presence and contamination of the pathogen by irradiating the measurement light to the viewpoint, and by measuring / analyzing the scattered light generated from the viewpoint.

At this time, the optical measurement sensor system receives the scattered light generated from the microfluidic chip 320 using an optical fiber to perform measurement / analysis. It is connected to a personal computer or an analysis / diagnostic terminal 400 through an interface, and outputs measurement / analysis information to provide a monitoring function for related information.

As shown in FIG. 20, the particle immune coagulation reaction scattering light measuring apparatus 300 according to the present invention includes a light source unit 310, a microfluidic chip 320, an optical path 330, a CCD array sensor 340, and a microprocessor. 350 and the communicator 360.

The light source 310 irradiates the measurement light to the microfluidic chip 320, more precisely, the viewpoint of the microfluidic chip in which the immunoaggregation reaction occurs. More preferably, an LED light source may be adopted as the light source unit () according to the present invention.

The microfluidic chip 320 has respective channels into which the test substance and the antibody stator particles can be introduced, and the microfluidic chip 320 is input to each channel to irradiate a point at which the aggregation reaction between the test substance and the antibody stator particles occurs. The light is scattered. Here, the microfluidic chip 320 is a one-time use, and is preferably configured to be easily installed in the measuring device.

The optical path 330 transmits the scattered light scattered by the microfluidic chip 320 to the CCD array sensor 340, and more preferably, may be configured using an optical fiber to minimize the loss.

The CCD array sensor 340 receives the scattered light transmitted through the optical fiber 330, converts the scattered light into an electrical signal, and outputs the converted signal to the microprocessor 350.

The microprocessor 350 receives and analyzes an electrical signal output from the CCD array sensor 340 to obtain wavelength information of light in a specific region, and checks pathogens and contamination levels of the test material based on the wavelength information of the light, and scattered light. By analyzing the concentration according to the intensity of the control agent to transmit the pathogen and contamination of the test substance and the analyzed concentration information to a computer, a terminal or a management server according to a specific communication method. In this case, the computer or the terminal may be used as an output means for outputting the received information through the display means. Of course, if the measuring device 300 itself is provided with independent display means such as LED or LCD, it will output the measurement results through the provided display means. In addition, the wavelength information of the light may be a wavelength of 380nm, the specific communication method is one selected from among known communication methods such as USB, RS232, IrDA, Bluetooth, RF, TCP / IP, wireless Internet protocol, or the like, or There may be a plurality of communication methods.

The microprocessor 350 described above will be described in more detail. The microprocessor 350 includes an A / D converter (not shown) in which an analog converts an electrical signal into a digital signal, and scattered light in a standard state where the test substance is not immunized. Storage means (not shown) such as a memory for storing a standard characteristic curve of the particle immune coagulation reaction consisting of the concentration information corresponding to the standard intensity and intensity for the The intensity and standard intensity are compared, and the pathogen and contamination level of the test substance are checked according to the comparison result, and the concentration according to the intensity of the scattered light is analyzed based on the standard characteristic curve.

The communication unit 360 communicates with a computer, a terminal, or a management server using a specific communication method under the control of the microprocessor 350, and transmits the measurement result (identified pathogen, contamination level, concentration information, etc. of the test substance) to the computer or Transfer to terminal or management server. As described above, the communication unit may be configured to perform communication according to one or a plurality of communication methods, and in this case, a communication means capable of supporting the adopted communication method is provided.

The light source unit 310, the CCD array sensor 340, and the microprocessor 350 of the above-described components may be provided in a dark type in which external light does not enter, so that the intensity and concentration of scattered light may be accurately measured and analyzed. More preferably.

Hereinafter, to describe the operation process of the particle immune coagulation reaction scattered light measuring apparatus 300 according to another embodiment of the present invention configured as described above.

First, the microfluidic chip 320 is fixed to the measuring device 300, and then a test substance and antibody stator particles are introduced into each channel.

Thereafter, the microprocessor 350 controls the light source unit 310 to irradiate the measurement light to a viewpoint where aggregation reaction between the test substance and the antibody stator particles occurs, and the microfluidic chip 320 scatters the emitted light by scattering the irradiated light. Generates. In this case, the scattered light is different in intensity of the scattered light according to the presence and concentration of the pathogen of the test material. That is, the intensity of the scattered light is changed according to the degree of the immunoaggregation reaction, and the measuring device according to the present invention measures the presence and concentration of the pathogen of the test substance by using these characteristics.

The microprocessor 350 receives scattered light scattered through the microfluidic chip 320 using an optical fiber, converts the received optical signal into an electrical signal, and then converts wavelength information of light belonging to a specific region (wavelength band). You get it. When the wavelength information of the light belonging to the specific region is obtained, the microprocessor 350 generates the measurement result by calculating the presence and concentration of the pathogen of the test substance with reference to the standard characteristic curve stored in the memory.

When the measurement is completed, the microprocessor 350 transmits the measurement result to the computer or the terminal connected through the interface such as a USB cable or a serial cable using the communication unit 360, or a wired / wireless network independently of the measuring device itself. When a communication unit 360 capable of data communication is provided through the wired / wireless network, the measurement result is transmitted to a computer, a terminal, or a management server.

As the present invention can be variously modified by those skilled in the art without departing from the scope of the claims, the technical protection scope of the present invention is not limited to the specific preferred embodiments described above. Do not.

1, 100, 200, 300: Portable measuring device
400: diagnostic terminal 410: network
420: disease management server 422: database
430: related agency server 432: database
1,100, 200: Immunoaggregated Scattered Light and PDA Fluorescence Biochip Measuring Device
10,110 body
11,111: chip loading part 12,112: microfluidic chip
20,120: light source unit 21: first light source
22: second light source 23: ferrule
30: filter slider 40: optical path unit
41: inclined ferrule 42: upright ferrule
43: second optical fiber 50: detection unit
51 detection unit ferrule 52: condensing lens
53 filter wheel assembly 53a: filter wheel
53b: filter 54: photodiode
60,150 control unit 80,170 display unit
130: spectroscope 131: spectroscope body
131a: light inlet 132: condensing slit
133: first reflecting mirror 134: second reflecting mirror
135: third reflector 136: detection unit
140: electrical signal conversion unit 160: communication unit
70: first optical fiber

Claims (28)

Portable measuring device for generating and transmitting the measurement results by measuring the presence and concentration of the pathogen of the test material using the optical characteristics; And
A disease management server for receiving a measurement result transmitted from the portable measuring device and storing the result in a database, and analyzing the received measurement result and transmitting an alarm message according to preset setting information when it is determined that the test substance is infected with a pathogen. Disease diagnosis / management system using a portable measuring device comprising a.
The method of claim 1,
The alarm message is a disease diagnosis / management system using a portable measuring device, characterized in that it comprises at least one or more of a message transmitted to the administrator's mobile terminal via a mobile communication network, the message transmitted to the administrator terminal through the network.
The method of claim 1,
Unique identification information is stored in the portable measurement device, and the measurement result transmitted from the portable measurement device includes the unique identification information.
The disease management server may be configured to determine at least one or more information among local information, affiliated organization information, and measurer information of the corresponding measurement device by using the unique identification information of the portable measurement device included in the received measurement result. Disease diagnosis / management system using the device.
The method of claim 1,
Disease diagnosis / management system using the portable measuring device,
Receiving and storing the alarm message is transmitted from the disease management server, the disease diagnosis / management system using a portable measuring device further comprises a related organ server for propagating the alarm message according to a preset procedure.
The method of claim 1,
The portable measuring device is an immunocoagulated scattering light and PDA fluorescent biochip measuring device,
The immuno-aggregated scattered light and PDA fluorescence biochip measuring device,
A chip loading unit in which a measurement sample is mounted;
A light source unit including at least one light source for supplying measurement light;
The spectacle is connected to any one of the light sources included in the light source unit according to the operator's operation, and supplies the light for measurement supplied from the connected light source to the measurement sample, receives the light passing through the measurement sample, and supplies the light to the detection unit. Optical path means for forming a furnace; And
And a detection unit for detecting light transmitted through the optical path means.
The method of claim 5,
The light source unit includes:
A first light source for supplying light for measuring immunocoagulated scattered light;
A second light source for supplying light for PDA embolism reaction measurement;
A first ferrule installed above the first light source to which the optical path means is connected / fixed; And
And a second ferrule installed above the second light source to connect / fix the optical path means.
The method of claim 6,
The light source unit may further include a filter slider disposed between the second light source and the second ferrule to bypass only light of a specific wavelength band among the light supplied from the second light source and to supply the light path to the optical path means. Disease diagnosis / management system using a portable measuring device.
The method of claim 5,
The optical path means,
A first optical fiber connected to any one of the light sources according to an operator's operation, the first optical fiber supplying light for measurement with a measurement sample mounted on the chip loading unit; And
And a light path unit for supplying the light passing through the measurement sample mounted to the chip loading unit to the detection unit.
The method of claim 8,
The light path unit,
A ferrol attached to one side of the chip loading part to receive light passing through the measurement sample and to supply the optical fiber to the connected optical fiber; And
And a second optical fiber for supplying light supplied to and connected to the ferrol to the detection unit.
10. The method of claim 9,
The ferrule,
An inclined ferrule configured to be detachably attached to one side of the chip loading part, and to receive scattered light passing through a measurement sample when the chip loading part is attached to one side of the chip loading part according to a selection of a measurer;
It is detachably configured on one side of the chip loading part, and when attached to one side of the chip loading part according to the selection of the measurer, the chip loading part is attached to one side of the chip loading part according to the choice of the measurer among the upright ferrules. Disease diagnosis / management system using a portable measuring device, characterized in that any one.
The method of claim 5,
Wherein:
A detection unit ferrule to which the optical fiber of the optical path means is fixed;
A condensing lens installed at one side of the detection unit ferrule and condensing light transmitted through the optical fiber;
A filter wheel for passing only a specific wavelength of light transmitted through the condenser lens; And
And a photodiode for receiving the light transmitted through the filter, converting the light into a current signal, and outputting the light signal.
The method of claim 11,
The filter wheel comprises at least one filter, the disease diagnosis / management system using a portable measuring device, characterized in that it is operated to position a particular filter between the condenser lens and the photodiode according to the operator's operation.
The method of claim 12,
The filter wheel,
Transmission filter for measuring immunocoagulated scattered light; And
A disease diagnosis / management system using a portable measuring device, characterized in that it comprises a bandpass filter for measuring the PDA embolism response.
The method of claim 1,
The portable measuring device is an immunocoagulated scattering light and a PDA fluorescent biochip measuring device using a spectrometer,
Immunoaggregated scattered light and PDA fluorescence biochip measuring device using the spectrometer,
A chip loading unit in which a measurement sample is mounted;
A light source unit including at least one light source for supplying measurement light;
A first optical fiber connected to the light source unit to irradiate light supplied from the light source unit with a measurement sample mounted to the chip loading unit;
A second optical fiber receiving light passing through the measurement sample and supplying the light to a spectroscope;
A spectrometer that separates the light supplied from the second optical fiber for each wavelength and detects and outputs light of a specific band;
An electrical signal conversion unit for converting the optical signal input from the spectroscope into an electrical signal and outputting the light detected by the spectroscope; And
And a control unit for determining pathogens and contamination levels of the measurement sample based on wavelength information of light of a specific region obtained by analyzing the electrical signal.
The method of claim 14,
The spectroscope,
A spectroscope body having a light inlet through which an optical fiber is fixed at one side;
A light collecting slit installed inside the body of the spectroscope and collecting light introduced into the light inlet into one place;
A first reflecting mirror installed inside the spectrometer body and reflecting light transmitted from the condensing slit;
A second reflector installed inside the spectrometer body and reflecting the light transmitted from the first reflector by wavelength band;
A third reflector installed inside the spectrometer body and reflecting light transmitted from the second reflector;
And a detection unit installed inside the body of the spectroscope and detecting the light transmitted from the third reflector.
The method of claim 14,
The light source unit includes a light source for measuring immunoaggregated scattered light and a light source for measuring PDA embolism reaction.
The first optical fiber is connected to any one of the light source for the immuno-aggregated scattering light measurement and PDA embolism response measurement according to the operator's operation, the disease diagnosis / management system using a portable measuring device.
The method of claim 1,
The portable measuring device is an immunocoagulated scattering light and PDA fluorescent biochip measuring device,
The immuno-aggregated scattered light and PDA fluorescence biochip measuring device,
A chip loading unit in which a measurement sample is mounted;
A light source unit coupled to a light source module according to a selection of a measurer among at least one light source module to supply light for measurement;
A light path connected to the light source unit to supply the measurement light supplied from the light source module selected / combined according to the measurement of the measurer to the measurement sample, and receive light passing through the measurement sample to supply to the detection unit; Light path means; And
And a detection unit for detecting light transmitted through the optical path means.
The method of claim 17,
The light source module may be any one of a first light source module for supplying light for measuring immuno-aggregated scattering light or a second light source module for supplying light for PDA embolism reaction measurement. system.
The method of claim 17,
The light source unit includes:
A specific light source module is coupled / separated according to the operator's operation, and includes a light source module coupler configured to supply measurement light output from the coupled light source module; And
And a light source unit ferrule installed above the light source module coupling unit to which the light path means is connected / fixed.
The method of claim 17,
The light path means,
A first optical fiber connected to the light source unit ferrule to supply light for measuring a measurement sample mounted to the chip loading unit; And
And a light path unit for supplying the light passing through the measurement sample mounted to the chip loading unit to the detection unit.
The method of claim 20,
The light path unit,
A ferrol attached to one side of the chip loading part to receive light passing through the measurement sample and to supply the optical fiber to the connected optical fiber; And
It includes a second optical fiber for supplying the light supplied to the detection connected to the ferrol,
The ferrule is detachably configured on one side of the chip loading part, and when attached to one side of the chip loading part according to the selection of a measurer, the inclined ferrule receiving the scattered light passing through the measurement sample, and detachable on one side of the chip loading part. It is configured to be attached to one side of the chip loading portion according to the selection of the measuring device is a portable measurement, characterized in that any one attached to one side of the chip loading portion according to the selection of the measuring device of the upright ferrule is received light passing through the measurement sample Disease diagnosis / management system using the device.
The method of claim 17,
The detector is a single diode detector,
The single diode detector,
A detection unit ferrule to which the optical fiber of the optical path means is fixed;
A filter wheel for passing only a specific wavelength of light transmitted through the detection buferol; And
And a photodiode for receiving the light transmitted through the filter, converting the light into a current signal, and outputting the light signal.
The method of claim 22,
The filter wheel comprises at least one filter, the disease diagnosis / management system using a portable measuring device, characterized in that the operation is operated so that a particular filter is located between the detection unit ferrule and the photodiode according to the operator's operation.
The method of claim 13,
The filter wheel,
Transmission filter for measuring immunocoagulated scattered light; And
A disease diagnosis / management system using a portable measuring device, characterized in that it comprises a bandpass filter for measuring the PDA embolism response.
The method of claim 17,
The detector is a spectrometer detector,
The spectrometer detector,
A detection unit ferrule to which the optical fiber of the optical path means is fixed;
A spectrometer for passing only a specific wavelength of light transmitted through the detection buferol; And
And an electrical signal converting unit converting light of the measurement band output from the spectrometer into an electrical signal and outputting the electrical signal.
The method of claim 1,
The portable measuring device is a particle immune coagulation reaction scattered light measuring device,
The particle immune aggregation reaction scattered light measuring apparatus,
A light source unit for irradiating light;
A microfluidic chip that scatters the irradiated light at a point where aggregation reaction between a test substance and antibody stator particles occurs;
An optical fiber for transmitting scattered light scattered from the microfluidic chip and a CCD array sensor for converting the transmitted scattered light into an electrical signal;
Communication unit for transmitting the characteristic information of the test substance and the concentration information according to the intensity of the scattered light by a specific communication method; And
Analyze the electrical signal to obtain light wavelength information of a specific region, identify the pathogen and contamination of the test material by the light wavelength information, analyze the concentration according to the intensity of the scattered light pathogens of the test material And a microprocessor for controlling to transmit pollution degree and analyzed concentration information based on the specific communication method.
The method of claim 26,
The wavelength information of the light is a disease diagnosis / management system using a portable measuring device, characterized in that the wavelength of 380nm.
The method of claim 26,
The microprocessor,
A converter for converting and outputting the analog electrical signal into a digital signal; And
It includes a memory that stores the standard characteristic curve of the particle immune coagulation reaction consisting of the concentration information corresponding to the standard intensity and intensity for the scattered light in the standard state without the pathogen for the test material,
Comparing the intensity corresponding to the wavelength information of the light obtained by conversion into the digital signal and the standard intensity, to determine the pathogen and contamination of the test material according to the comparison result, the scattered light based on the standard characteristic curve Disease diagnosis / management system using a portable measuring device, characterized in that for analyzing the concentration according to the intensity.

Images