KR101139954B1 - Apparatus and method for measuring intensity of particle immunoagglutination reaction - Google Patents

Abstract

The present invention relates to a scattering light measuring device and method for particle immune agglutination reaction, specifically, the pathogen and contamination of the test material can be identified through the light scattered by irradiating light to a site where the agglomeration reaction between the test material and the antibody immobilized particles occurs. The present invention relates to an apparatus and a method for measuring scattered light of particle immune agglomeration. Such a present invention is a light source unit for irradiating light; A microfluidic chip that scatters the irradiated light at a point where aggregation reaction between the test substance and the antibody stator particles occurs; An optical fiber for transmitting scattered light scattered from the microfluidic chip; A CCD array sensor for converting the transmitted scattered light into an electrical signal; Communication unit for transmitting the characteristic information of the test substance and the concentration information according to the intensity of the scattered light by a specific communication method; And analyzing the electrical signal to obtain wavelength information of light of a specific region, confirming pathogens and contamination levels of the test material based on the wavelength information of the light, and analyzing concentrations according to the intensity of the scattered light. It provides a particle immune agglutination reaction scattering light measuring apparatus and method comprising a microprocessor for controlling the transmission of pathogens, contamination and analyzed concentration information based on the specific communication method.

Description

Apparatus and method for measuring particle immune coagulation reaction light scattering light {APPARATUS AND METHOD FOR MEASURING INTENSITY OF PARTICLE IMMUNOAGGLUTINATION REACTION}

The present invention relates to a scattering light measuring device and method for particle immune agglutination reaction, specifically, the pathogen and contamination of the test material can be identified through the light scattered by irradiating light to a site where the agglomeration reaction between the test material and the antibody immobilized particles occurs. The present invention relates to an apparatus and a method for measuring scattered light of particle immune agglomeration.

At present, Korean people are increasing their interest in welfare and health as the economy grows toward the developed countries. In particular, since 2006, various projects have been in progress, especially in the IT field, at the national level on u-health.

In terms of u-Health service demand, no matter what type of respiratory illness, only one type of respiratory disease can be used to motivate users to voluntarily motivate their health care and prevention or to change behavior by discovering new health indexes through continuous and periodic measurements. It is expected to welcome both the normal person and his family, as well as the hospital and the patient, as it can induce active participation of the user.

Therefore, u-Health service with fusion / composite sensor is the representative realization of ubiquitous information communication culture, and it is emerging as the industrial area that will enrich our life. The Korea Information Society Agency (KISA) published the Korean Ubiquitous Society Research Series No. 17 'Ubiquitous Society's Medical / Bot-Vineness Trend' report on July 3, 2006 to continuously accumulate and manage human information due to the aging of the population and the increase of chronic diseases. As the necessity emerges, it is analyzed that the consumer demand for traditional health Paradigm is changing to 'Ubiquitous Health Care'.

The domestic u-Health Care service user market is expected to reach about 6.26 million after five years and the sales volume will exceed 1 trillion won.

Therefore, developed countries are focusing on IT-based medical and health services through competitive and active market transactions, centering on the strengths of their industries. The u-Health Care service is expected to continue in the future, driven by the improvement of consciousness to improve the quality of life and the development of IT technology.

In addition, u-Health services related to converged / composite air pollution sensor technology are underway in the EU Health Care Project and various service cases at home and abroad.

On the other hand, due to the rapid increase in the consumption of various agricultural and livestock products, the production of agricultural and livestock products is being produced in large quantities, and the import of various food ingredients has explosively increased, thereby strengthening the inspection for the safety management of various foods.

In particular, agricultural and livestock products are directly related to national health, and thorough management and supervision are required from the birth stage to the distribution process.

However, it is not easy to equip expensive inspection equipment to ensure the stability of these foods, and because the inspection means are complicated, various kits for easily detecting contamination of agricultural and livestock products are being developed. It is true.

Therefore, there is a need to develop a device capable of detecting various pathogens simply and accurately in place of expensive inspection equipment that requires complicated and highly skilled labor.

There is a need to easily and conveniently detect a variety of pathogens generated in livestock farms and pathogens contaminated during washing of agricultural products through a portable inspection device developed according to this need.

The present invention has been made in view of the necessity as described above, the particle immune coagulation to check the pathogen and contamination of the test material through the light scattered by irradiating light to the site where the aggregation of the test material and the antibody immobilized particles occurs It is an object of the present invention to provide an apparatus and a method for measuring reaction scattered light.

Another object of the present invention is to provide an apparatus and a method for measuring particle immune agglutination reaction scattered light, which is small and easy to carry, so that pathogens and contamination levels of test substances can be diagnosed in real time.

Technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the following description.

Particle immune coagulation reaction scattered light measuring apparatus according to an aspect of the present invention for achieving the above object, the light source unit for irradiating light; A microfluidic chip that scatters the irradiated light at a point where aggregation reaction between the test substance and the antibody stator particles occurs; An optical fiber for transmitting scattered light scattered from the microfluidic chip; A CCD array sensor for converting the transmitted scattered light into an electrical signal; Communication unit for transmitting the characteristic information of the test substance and the concentration information according to the intensity of the scattered light by a specific communication method; And analyzing the electrical signal to obtain wavelength information of light of a specific region, confirming pathogens and contamination levels of the test material based on the wavelength information of the light, and analyzing concentrations according to the intensity of the scattered light. It characterized in that it comprises a microprocessor for controlling the transmission of pathogens, contamination and analyzed concentration information based on the specific communication method.

Preferably, the wavelength information of the light is characterized in that the wavelength is 380nm.

Preferably, the microprocessor, the converter for converting the analog electrical signal to a digital signal; And a memory for preliminarily storing a standard characteristic curve of the particle immune agglutination reaction comprising concentration information corresponding to standard intensity and intensity for scattered light in a standard state in which no pathogen is present for the test substance. Compare the intensity corresponding to the wavelength information of the light obtained by the change and the standard intensity, and confirm the pathogen and contamination of the test material according to the comparison result, the concentration according to the intensity of the scattered light based on the standard characteristic curve Characterized in that the analysis.

Preferably, the specific communication method is characterized in that one of the USB, RS232 or IrDA communication method.

Preferably, the light source unit, the CCD array sensor and the microprocessor is characterized in that it is provided in the dark room does not receive external light.

Preferably, the microfluidic chip is disposable, and is easy to replace.

According to another aspect of the present invention, a method for measuring particle immune agglutination reaction scattered light may include: irradiating light to a point of a microfluidic chip in which an agglomeration reaction between a test substance and antibody stator particles occurs; The irradiated light is scattered by a particle immune coagulation reaction, receives the scattered scattered light and converts the scattered light into an electrical signal, and analyzes the electrical signal to obtain wavelength information of light in a specific region, and the wavelength information Identifying a pathogen and a degree of contamination of a test substance and analyzing the concentration according to the intensity of the scattered light; And transmitting the pathogen and the contamination degree and the analyzed concentration information of the test substance by a specific communication method.

Preferably, the wavelength information of the light is characterized in that the wavelength is 380nm.

Preferably, analyzing the concentration according to the intensity of the scattered light, converting the analog electrical signal into a digital signal; Comparing the intensity corresponding to the wavelength information of the light obtained by converting the digital signal with a standard intensity; And confirming the pathogen and contamination of the test substance according to the comparison result and analyzing the concentration.

Preferably, the step of analyzing the concentration, characterized in that for analyzing the concentration according to the intensity of the scattered light on the basis of the standard characteristic curve of the particle immune agglomeration reaction consisting of a concentration corresponding to the intensity.

Preferably, the standard intensity is an intensity for scattered light in a standard state in which no pathogen is present for the test substance, and the standard intensity is pre-stored together with the standard characteristic curve.

Preferably, the specific communication method is characterized in that one of the USB, RS232 or IrDA communication method.

Preferably, the pathogen and the degree of contamination of the test material from the scattered light, and the analysis of the concentration according to the intensity is characterized in that in the dark room does not receive external light.

Preferably, the microfluidic chip is characterized in that it is easy to replace as a single use.

According to the above-described problem solving means, the present invention enables to accurately measure the intensity of scattered light generated by irradiating light to a site where aggregation reaction between test substance and antibody immobilized particle occurs, thereby increasing the reliability of pathogen diagnosis and contamination diagnosis. It works.

In addition, it can be miniaturized to be portable so that it is easy to diagnose pathogens and contamination levels in real time anytime and anywhere.

1 is an exemplary view for schematically illustrating an apparatus for measuring particle immune coagulation reaction scattered light according to an embodiment of the present invention.
Figure 2 is a block diagram of a device for measuring the particle immune coagulation reaction scattered light according to an embodiment of the present invention.
Figure 3 is an exemplary view showing the structure of the particle immune coagulation reaction scattering light measuring apparatus according to the present invention.
4 and 5 are a flow chart showing a method for measuring scattered light of particle immune coagulation reaction according to an embodiment of the present invention.

In the following description, specific details of the apparatus and method for measuring particle immune agglutination reaction scattering light of the present invention are shown to provide a more general understanding of the present invention, and the present invention can be easily carried out without these specific details and by their modifications. It will be apparent to one of ordinary skill in the art.

On the other hand, the particle immune agglutination reaction scattering light measuring system according to the present invention is applicable to all fields (virus, bacteria, antibiotics, heavy metals, GMO genes, etc.) that induce antigen-antibody reactions and dozens of The target of can be detected simultaneously. In particular, by using optical fibers using light, food safety management may be used as well as denaturation of antibodies due to detection.

Hereinafter, with reference to the accompanying drawings, preferred embodiments of the present invention will be described in detail, focusing on the parts necessary to understand the operation and action according to the present invention.

1 is an exemplary view for schematically explaining a device for measuring the particle immune coagulation reaction scattered light according to an embodiment of the present invention.

Referring to FIG. 1, an apparatus for measuring particle immune coagulation reaction scattering light according to the present invention includes a microfluidic chip 50 that measures and analyzes a microfluidic chip, an optical fiber connected to the measuring device, and an intensity and a concentration of scattered light incident thereto. It consists of an optical measurement sensor system.

First, the microfluidic chip has a channel for introducing a test substance and a channel for inputting antibody-immobilized nanoparticles, and a light source is provided at a view point of a site where the two channels are combined to cause an aggregation reaction by an antigen antibody reaction. It is composed of a chip capable of measuring scattered light by irradiating a source).

The scattered light generated at this time is fixed to a predetermined position using an optical fiber to process the generated signal in the optical measuring sensor system.

Thereafter, the optical measurement sensor system is connected to a computer or a terminal through a USB interface and configured to monitor output data of the optical measurement sensor system.

It will be described the configuration of the particle immune agglomeration reaction scattering light measuring device that performs this operation and action.

2 is a block diagram of an apparatus for measuring particle immune agglutination reaction scattered light according to an embodiment of the present invention.

Referring to FIG. 2, the apparatus for measuring particle immune aggregation reaction scattered light according to the present invention includes a light source unit 10, a microfluidic chip 20, an optical fiber 30, a CCD array sensor 40, and a microcontroller. It is configured to include a processor 50, the communication unit 60 and the like.

The light source unit 10 irradiates light. The light source unit 10 may be a light source made of LED.

The microfluidic chip 20 has respective channels into which the test substance and the antibody stator particles can be introduced, and the microfluidic chip 20 is inserted into each channel and irradiated to the spot where the aggregation reaction between the test substance and the antibody stator particles occurs. Scatter the light. Here, the microfluidic chip 20 is for one-time use, and it is preferable that the microfluidic chip 20 is installed in the particle immune agglomeration reaction scattering light measuring device easily.

The optical fiber 30 transmits the scattered light scattered by the microfluidic chip 20 to the CCD array sensor 40 side.

The CCD array sensor 40 converts the scattered light transmitted through the optical fiber 30 into an electrical signal.

The microprocessor 50 receives an electrical signal from the CCD array sensor 40 and analyzes the signal to obtain wavelength information of light in a specific region, checks pathogens and contamination levels of the test material based on the wavelength information of the light, and intensity of scattered light. Analyze the concentration according to the control to transmit the pathogen and contamination level of the test substance to the computer or terminal based on the specific communication method. In this case, the computer or the terminal may be a means for displaying the received information on the screen. The wavelength information of the light may be a wavelength of 380nm, the specific communication method is one of the USB, RS232 or IrDA communication method.

The microprocessor 50 will be described in more detail. The microprocessor 50 includes a converter (not shown) for converting an analog electrical signal into a digital signal, and a standard for scattered light in a standard state where the test substance is not immunized. It includes a memory (not shown) that pre-stores the standard characteristic curve of the particle immune coagulation reaction consisting of the intensity and the concentration information corresponding to the intensity, and the intensity and standard intensity corresponding to the wavelength information of the light obtained by changing into a digital signal Compare and check the pathogen and contamination level of the test substance according to the comparison result, and analyze the concentration according to the intensity of the scattered light based on the standard characteristic curve.

The communication unit 60 performs communication with a computer or a terminal connected through a communication connector (not shown) according to a specific communication method under the control of the microprocessor 50 to provide concentration analysis information along with confirmed pathogens and contamination levels of the test substance. Transfer it to your computer or terminal. The computer or terminal then displays the received information.

The light source unit 10, the CCD array sensor 40, and the microprocessor 50 described above may be provided in a dark room where external light does not enter, so that the intensity and concentration of scattered light may be accurately measured and analyzed. do.

The above-described particle immune coagulation scattering light measuring apparatus 100 may be manufactured in the shape and configuration as shown in FIG. In FIG. 3, the reference numeral '70' indicates a communication connector connected to an external computer or terminal.

4 is a flowchart illustrating a method for measuring scattered light of particle immune agglomeration according to an embodiment of the present invention. Herein, in order to confirm the immunity of the test substance by the method of measuring particle immune agglutination reaction scattered light according to FIG. 4, the particle immune agglomeration consisting of concentration information corresponding to the standard intensity and intensity of the scattered light in a standard state in which the test substance is not immunized The standard characteristic curve of the reaction should be stored in advance. This storage step is omitted in the drawings.

Referring to FIG. 4, first, a test substance and antibody stator particles are introduced into each channel of the microfluidic chip 20 (S410).

Thereafter, the microprocessor 50 controls the light source unit 10 to irradiate light to a view point of a site where aggregation reaction between the test substance and the antibody stator particles occurs (S420). The microfluidic chip 20 then scatters the irradiated light. In this case, the scattered light has a different intensity of scattering of light depending on the presence and concentration of the pathogen of the test substance.

Then, the microprocessor 50 receives the scattered scattered light through the optical fiber 10 and converts the scattered light into an electrical signal (S430), analyzes the electrical signal to obtain wavelength information of light in a specific region, and examines the test material based on the wavelength information. Check the pathogen and contamination of the (S440). At this time, identify the pathogen and analyze the concentration according to the intensity of the scattered light. Here, the process of identifying the pathogen of the test substance from the scattered light and the process of checking the intensity of the scattered light is preferably performed in a dark room where external light does not enter.

Thereafter, the microprocessor 50 transmits the analyzed concentration information together with the confirmed pathogen and contamination of the test substance to the computer or the terminal by a specific communication method (S450).

Referring to the step (S440) of confirming the characteristics and immunity of the test substance described above in detail, as shown in Figure 5, the microprocessor 50 first converts the analog electrical signal to a digital signal (S441) .

Thereafter, the microprocessor 50 compares whether the intensity corresponding to the wavelength information of the light obtained by changing into a digital signal is equal to the pre-stored standard intensity (S443).

As a result of the comparison, if the intensity corresponding to the wavelength information of the obtained light and the standard intensity is the same, the microprocessor 50 determines that the pathogen is a normal test substance that does not exist (S445).

However, if the intensity and standard intensity corresponding to the wavelength information of the light obtained as a result of the comparison is not the same, the microprocessor 50 determines that the pathogen is a test substance present (S447).

After performing steps 445 and 447, the microprocessor 50 analyzes the concentration according to the intensity of the scattered light based on the stored standard characteristic curve (S449).

Through this process, the result data on the identified pathogen and the analyzed concentration of the test substance is transmitted to the computer or the terminal side connected by a specific communication method (S450). At this time, the microprocessor 50 detects whether a computer or a terminal is connected to the communication connector 70.

The computer or the terminal then displays the data received by the specific communication method through the display means.

While the present invention has been described in connection with what is presently considered to be the most practical and preferred embodiment, it is to be understood that the invention is not limited to the disclosed embodiments, but is capable of various modifications within the scope of the invention. Therefore, the scope of the present invention should not be limited by the illustrated embodiments, but should be determined by the scope of the appended claims and equivalents thereof.

Claims (14)

A light source unit for irradiating light;
A microfluidic chip having a channel for introducing a test substance and a channel for inputting antibody-immobilized nanoparticles, and scattering the irradiated light at a point where aggregation reaction between the test substance and the antibody stator particles occurs;
An optical fiber for transmitting scattered light scattered from the microfluidic chip; A CCD array sensor for converting the transmitted scattered light into an electrical signal;
Communication unit for transmitting the characteristic information of the test substance and the concentration information according to the intensity of the scattered light by a specific communication method; And
Analyze the electrical signal to obtain wavelength information of light of a specific region, identify pathogens and contamination levels of the test material based on the wavelength information of the light, analyze the concentration according to the intensity of the scattered light pathogens of the test material And a microprocessor which controls to transmit the pollution degree and the analyzed concentration information based on the specific communication method.
The microprocessor includes a particle immune agglomeration reaction comprising a converter for converting the analog electrical signal into a digital signal and concentration information corresponding to the standard intensity and intensity for scattered light in a standard state in which no pathogen is present for the test substance. Includes memory to pre-store standard characteristic curves,
The microprocessor compares the intensity corresponding to the wavelength information of the light obtained by changing to the digital signal and the standard intensity, and confirms the pathogen and contamination of the test substance according to the comparison result, and the standard characteristic curve Particle immune coagulation reaction scattered light measuring apparatus, characterized in that for analyzing the concentration according to the intensity of the scattered light.
The method of claim 1, wherein the wavelength information of the light,
Particle immune coagulation reaction scattered light measuring apparatus, characterized in that the wavelength is 380nm.
delete The method of claim 1, wherein the specific communication method,
Particle immune coagulation reaction scattered light measuring device, characterized in that the USB, RS232 or IrDA communication system.
The apparatus of claim 1, wherein the light source unit, the CCD array sensor, and the microprocessor are provided in a dark room to which external light does not enter.
The method of claim 1, wherein the microfluidic chip,
Particle immune coagulation reaction scattered light measuring device, characterized in that the replacement is easy for single use.
Irradiating light to a spot of a microfluidic chip in which an agglomeration reaction between the test substance and the antibody stator particles occurs;
The irradiated light is scattered by a particle immune coagulation reaction, receives the scattered scattered light and converts the scattered light into an electrical signal, and analyzes the electrical signal to obtain wavelength information of light in a specific region, and the wavelength information Identifying a pathogen and a degree of contamination of a test substance and analyzing the concentration according to the intensity of the scattered light; And
Including the step of transmitting the pathogen and contamination degree and the analyzed concentration information of the test substance by a specific communication method,
Analyzing the concentration according to the intensity of the scattered light, converting the analog electrical signal into a digital signal, comparing the intensity and the standard intensity corresponding to the wavelength information of the light obtained by changing to the digital signal And confirming the pathogen and contamination of the test substance according to the comparison result and analyzing the concentration.
Analyzing the concentration is to analyze the concentration according to the intensity of the scattered light on the basis of the standard characteristic curve of the particle immune agglomeration reaction made of a concentration corresponding to the intensity,
The standard intensity is the intensity of the scattered light in a standard state in which no pathogen is present for the test substance, and is pre-stored together with the standard characteristic curve.
The method of claim 7, wherein the wavelength information of the light,
Particle immune coagulation reaction scattered light measurement method, characterized in that the wavelength is 380nm.
delete delete delete The method of claim 7, wherein the specific communication method,
Particle immune coagulation reaction scattered light measurement method characterized in that the USB, RS232 or IrDA communication method.
The method according to any one of claims 7, 8, and 12, wherein the pathogen and contamination of the test substance are identified from the scattered light, and the concentration according to the intensity is performed in a dark room in which no external light enters. Particle immune aggregation reaction scattered light measurement method.
The method of claim 7, wherein the microfluidic chip,
Particle immune coagulation reaction scattered light measuring method characterized in that the replacement is easy as a single use.

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