KR20110131551A - Ultraviolet rays irradiator for enhancing osseointegration and surface treatment method of dental implant using the same - Google Patents

Abstract

PURPOSE: An ultraviolet ray irradiator for enhancing osseointegration and a surface treatment method of a dental implant using the same are provided to improve cell activation and osseointegration. CONSTITUTION: An ultraviolet ray irradiator for an implant comprises: a chamber(10) in which an optical irradiation space is formed; a door(20) which is installed in the chamber and opens and closes the optical irradiation space; a table(30) which is installed in the optical irradiation space of the chamber and in which an implant member is installed; a light source array module(50) which is installed in the chamber and in which a plurality of light sources are arrayed in a fixed pattern; and a rotary unit which rotates the implant member mounted on a table.

Description

Ultraviolet rays irradiator for enhancing osseointegration and surface treatment method of dental implant using the same}

The present invention relates to an ultraviolet light irradiator for bone adhesion enhancement and a method of modifying the dental implant surface using the same, and more particularly, by irradiating ultraviolet rays to the surface of the implant formed with titanium oxide film to increase the hydrophilicity on the implant surface and bone cells and The present invention relates to an ultraviolet light irradiator for enhancing bone adhesion that can enhance early bone bonding by increasing the affinity of and an implant surface modification method using the same.

As the aging society progresses, quality of life is emerging as a social issue, and attention to health is being subdivided. In particular, oral health has a direct impact on general health, but tooth loss due to aging is inevitable. .

Proper teeth treatment such as dentures and bridges is the main solution to tooth loss because it does not affect food intake and affects interpersonal relationships due to digestive problems, facial deformation and incorrect pronunciation. However, implant treatment, a treatment approach that is close to natural teeth, has emerged, and implant surgery is recognized as a universal treatment method for tooth loss due to the improvement of the income level of consumers and the development of dental dental technology.

Dental implants are used to permanently implant artificial teeth on the jaw bone of humans. The dental implant connects the jaw bone with artificial teeth and handles and distributes the load generated when chewing food. It is mechanically manufactured to serve as a more stable tooth than a conventional denture. Therefore, the implant should be made of biocompatible materials that are very stable against human tissues and should be free of side effects and other chemical and biochemical reactivity. In addition, it is very difficult to select a suitable material because the mechanical strength must be very high so as not to be deformed and destroyed even under repeated loads and instantaneous pressures.

Various metals and alloys have been developed and tried as suitable materials for implants, but Ti (titanium) metals and alloys are mainly used (Larry L. Hench, "Bioceramics", J. Am. Ceram. Soc. 81 [7] 1705 -28, 1998). Ti or its alloys are not only easy to process but also have the advantages of high biocompatibility, high mechanical strength and bioinertness to human tissues. However, Ti and its alloys have a disadvantage in that the bonding time with the bone is long when transplanted into the human body, and metal ions melt into the living body after a long time after transplantation.

In order to make up for these drawbacks, techniques have been developed and used to enhance bone bonding by appropriately treating the surfaces of these materials. The rate and quality of bone bonding are closely related to the surface properties and chemical composition of the implant, such as surface composition, surface roughness, and hydrophilicity.In particular, implants with a high hydrophilic surface may be used to interact with biological solutions, cells, and tissues. It is known to be advantageous and to make good bone-media contact.

As one of the implant surface treatment techniques, TiO ₂ oxide film on Ti surface is reported to be stable in vivo, excellent in biocompatibility, and positive in reaction with cells. Bonding with bone has been reported to be excellent (Patrick J. Henry, Albert ES Tan, Brent P. Allan, Jan Hall and Carina Johansson, "Removal Torque Comparison of TiUnite and Turned Implants in the Greyhound Dog Mandible," Applied Osseointegration Research, 1 [1] 15-17, 2000).

However, since the oxide film naturally present on Ti and its alloy surface is only a few nanometers, it is a big problem to make the Ti oxide film to an appropriate thickness. Therefore, a method of forming the Ti oxide film by physical methods such as plasma spraying, sputtering, ion implantation, and the like is known. Fini et al. Reported that TiO ₂ oxides were formed by anodization under constant voltage without the occurrence of cracks (M. Fini, A. Cigada, G. Rondelli, "In vitro and in vivo behavior of Ca and P-enriched anodized titanium ", Biomaterials, 20, 1587-1594, 1999).

However, the oxide film formed by the above method has a disadvantage in that adhesive strength with bone is not sufficient and the process is complicated.

The present invention was created to improve the above problems, an ultraviolet light irradiator capable of improving cell activation and bone adhesion by modifying the titanium oxide film to hydrophilic by a simple process of irradiating the ultraviolet light to the titanium oxide film formed on the surface of the implant and this The purpose of the present invention is to provide a dental implant surface modification method.

Another object of the present invention is to provide an ultraviolet light irradiator capable of irradiating ultraviolet light uniformly over the entire surface of the implant when the surface of the implant is irradiated with ultraviolet rays and a dental implant surface modification method using the same.

And the ultraviolet light irradiator of the present invention for achieving the above object is a chamber with a light irradiation space therein; A door installed in the chamber to open and close the light irradiation space; A table installed in the light irradiation space of the chamber and having an implant member having a titanium oxide film formed on a surface thereof; A light source array module installed in the chamber and arranged in a pattern in which a plurality of light sources are installed to increase the hydrophilicity of the surface of the implant member by irradiating ultraviolet rays to the surface of the implant member; And rotating means for rotating the implant member mounted on the table so that ultraviolet rays can be uniformly irradiated onto the surface of the implant member.

The light source of the light source array module is characterized in that the light emitting diode having a 365nm wavelength.

The table is coupled to the chamber, the receiving space is formed therein, the top is open, the lower cover is formed with a plurality of first through holes at regular intervals, and is detachably coupled to the upper portion of the lower cover and the first through hole A top cover having second through-holes formed at positions corresponding to each of them, and a plurality of rotatable disks accommodated in a receiving space of the lower cover, and rotating disks that rotate simultaneously to the other when one rotates by the rotating means. Each of the rotating disks engages with an adjacent rotating disk and has a cog wheel formed larger than the diameter of the first and second through holes, and a first disc formed under the cog wheels and inserted into the first through hole. A second disc portion formed on an upper portion of the cog wheel and inserted into the second through hole and to which the implant member is mounted; It is formed in the group the second disc-shaped portion characterized in that a fixing means for preventing the flow of the second disk portion in a rotation of the implant member.

The rotating means is installed in the base portion for supporting the lower portion of the chamber and the drive shaft is accommodated so as to be rotatable in the accommodation space of the lower cover and the drive shaft is coupled to the drive shaft and the rotating disk A drive gear meshing with the gearwheel of any one of the rotating disks, the sensor unit being installed in the chamber to detect the opening / closing of the door and outputting a signal, and opening the door by an output signal of the sensor unit. It characterized in that it further comprises a control unit for generating a warning sound through the buzzer and at the same time cut off the power applied to the light source array module.

Implant surface modification method for promoting bone adhesion of the present invention for achieving the above object comprises the steps of mounting an implant member having a titanium oxide film formed on the surface of the table installed in the chamber irradiation space; Rotating the implant member mounted to the table; And irradiating ultraviolet rays to the surface of the implant member through a light source installed in the chamber to increase the hydrophilicity of the surface of the implant member.

The light source is a light emitting diode having a 365nm wavelength, the ultraviolet light is characterized in that irradiated for 3 to 24 hours at 2mW / cm ² to the implant member rotating at 10 to 60rpm.

As described above, according to the present invention, a hydrophilic property may be imparted to the surface of the implant member by a simple process of irradiating ultraviolet light to the titanium oxide film naturally formed on the surface or the implant member coated with the titanium oxide film artificially.

Thus, the absence of a hydrophilic surface-modified implant can promote interaction with biological solutions, cells and tissues to enhance early Osseointegration.

In addition, the ultraviolet irradiation process can be easily performed by developing a dedicated device for irradiating ultraviolet rays to the implant member. In particular, a plurality of implant members can be installed on the table, and the ultraviolet rays are irradiated while the implant members are rotated, so that a uniform film modification operation can be performed over the entire surface.

1 is an exploded perspective view of an ultraviolet light irradiator according to an embodiment of the present invention,
2 is a front view of FIG. 1,
3 is a cross-sectional view showing a main portion provided with an implant member on the table of FIG.
4 is a block diagram schematically showing the configuration of the ultraviolet light irradiator of FIG.
6 is a graph analyzing the surface residue of the specimen,
7 to 12 are photographs showing the appearance of the water droplets formed in the control group and the test group, respectively, and the average contact angle,
13 is a graph showing the absorbance of the control group and the test group,
14 to 18 are photographs showing images of cells attached to the control group and the test group at 250 magnification,
19 to 23 is a photograph showing an image of the cells attached to the control group and the test group at a 1000 magnification,
24 is a graph quantifying the number of cells attached to the control group and the test group.

 Dental implants generally refer to the replacement of the missing natural tooth itself or a screw-shaped fixture to the alveolar bone to fuse with the bone for a period of time and then to the abutment on the abutment. ) And a dental procedure for restoring the original function of a tooth by fixing a prosthesis such as an artificial crown. In the present invention, a dental implant means a substitute for a lost natural tooth. And in the present invention, the implant member means a fixture, abutment, fixing screw, artificial crown, etc. constituting a substitute for natural teeth, and particularly means a fixture placed in the alveolar bone. In addition to fixtures, all implant members that bind to bone cells can be applied.

As shown in FIG. 5, a substitute for natural teeth, that is, artificial teeth, is typically a fixture 3 inserted into the alveolar bone 1, an abutment 5 coupled to an upper portion of the fixture 3, and It consists of a fixing screw (7) for fixing the abutment (5) to the fixture (3), and an artificial crown (9) mounted on the abutment (5).

Hereinafter, with reference to the accompanying drawings will be described in detail an ultraviolet light irradiator for promoting bone adhesion according to a preferred embodiment of the present invention.

1 to 4, the ultraviolet light irradiator according to an embodiment of the present invention largely the chamber 10, the door 20, the table 30, the light source array module 50, the rotating means It is provided.

The chamber 10 has a structure in which a light irradiation space 11 is formed inside and an open front surface thereof. The open front door 20 is installed to open and close the light irradiation space (11). Door 20 is hinged to the bracket 13 formed on the upper portion of the chamber 10 is installed in the chamber 10 to be rotated up and down.

The base portion 25 supporting the chamber 10 is installed below the chamber 10. The base unit 25 is provided with a key operating unit 27 for operating the ultraviolet light irradiator of the present invention on one side, and a display unit 29 for outputting various types of information on the other side. The key operation unit 27 is composed of four buttons (MENU, UP / STRAT, DN / PAUSE, SET) in which the user can set the output value of the light source, the motor rotation speed, and the operation time. And the inside of the base portion 25 is provided with a controller and various electric circuits to be described later.

The table 30 is installed in the light irradiation space 11 formed inside the chamber 10. The table 30 may be fixed to the chamber 10 but is preferably installed to be coupled to or separated from the chamber 10. The implant member 3 is mounted to the table 30.

The implant member 3 may be formed of a titanium material or may be formed by coating a titanium layer on the outside of a substrate of a material other than titanium. The implant member 3 has a titanium oxide film TiO ₂ formed on its surface. The titanium oxide film may be naturally formed on the surface of the implant member in contact with air, or artificially form the titanium oxide film.

The present invention has a structure in which the implant member 3 mounted on the table 30 can be rotated by the rotating means. This is to make it possible to irradiate ultraviolet rays uniformly on the surface of the implant member (3).

The illustrated table 30 is accommodated in such a way that the lower cover 31, the upper cover 36 coupled to the upper part of the lower cover 31, and the receiving space 33 of the lower cover 36 are rotatable. Rotating disks 40 are provided.

The lower cover 31 has an accommodating space 33 formed therein and has an open top portion. The lower cover 31 is mounted to the chamber 10 to be coupled and detached. On the bottom of the lower table 31, two insertion protrusions (not shown) protruding vertically downward are formed. The insertion protrusion is inserted into two coupling holes 12 formed at the bottom of the chamber 10. The lower cover 31 is provided with a plurality of first through holes 35 at regular intervals. The first through holes 35 are all formed with the same diameter, but may be formed with different diameters.

The upper cover 36 has a structure in which the lower portion is open, and is coupled to the upper portion of the lower cover 31. Preferably, the upper cover 36 is coupled to the lower cover 31 to be detachable. To this end, elastic pieces 39 are formed on both side surfaces of the upper cover 36, respectively. One side of the elastic piece 39 is coupled to the upper surface of the upper cover 36 and the other three sides are formed in a square separated from the side. In the elastic piece 39, a rectangular locking hole 38 is formed long before and after. In addition, a locking protrusion 34 fitted to the locking hole 38 is formed at a side surface of the lower cover 31 at a position corresponding to the locking hole 38. Therefore, when the upper cover 36 is coupled to the lower cover 31, the elastic piece 39 is opened in the outward direction of the upper cover 36 by the locking protrusion 34, and the locking protrusion 34 is the locking hole ( 38, the locking piece 34 is inserted into the locking hole 38 while the elastic piece 39 is in close contact with the side surface of the lower cover 31 by the elastic force of the elastic piece 39.

A plurality of second through holes 37 are formed on the upper surface of the upper cover 36. The second through hole 37 is formed at a position corresponding to the first through hole 35. Therefore, each second through hole 37 is formed at a position facing the first through hole 35. The second through holes 37 are all formed with the same diameter, but may be formed with different diameters. The second through hole 37 is formed to have the same diameter as the first through hole 35 facing each other.

A plurality of rotating disks 40 are installed between the lower cover 31 and the upper cover 36. In the illustrated example, a plurality of rotating disks 40 are rotated in cooperation with each other, in contrast, each of the rotating disks 40 can be rotated independently. In addition, only one rotary disk 40 may be installed in addition to a plurality is installed, of course. In addition, the number of the rotating disk 40 may also be changed to various numbers, as shown.

In the example of the rotating disk 40 shown, each of the rotating disk 40 meshes with an adjacent rotating disk and is formed with a cogwheel 41 larger than the diameter of the first and second through holes 35 and 37. And a first disc portion 43 formed below the cog wheel 41 and inserted into the first through hole 35 and inserted into the second through hole 37 formed above the cog wheel 41. And a second disc portion 45 on which the implant member 3 is mounted, and a second disc portion 45 formed in the second disc portion 45 to prevent the flow of the implant member 3 during rotation of the second disc portion 45. It is provided.

Various techniques may be applied as the fixing means, but as an example, the fixing rod 47 may be formed at the center of the second disc portion 45. The fixing rod 47 is formed to extend at a predetermined height vertically on the upper surface of the second disc portion 45. The diameter of the fixing rod 47 may be applied in various sizes. In addition, the fixed rod 47 may be formed in various shapes such as square, triangle, pentagon, in addition to the circular cross section.

The fixing rod 47 is inserted into a hole formed in the implant member 3 to prevent the flow of the implant member 3 during rotation of the rotating disk 40. As shown in FIG. 3, when the fixture is applied as an example of the implant member 3, the implant member 3 may be inserted into a screw hole formed in the fixture.

Rotating means for rotating the rotating disk 40 is installed in the base portion 25 and the electric motor 60, the drive shaft 61 extending into the interior of the chamber 10, the lower cover 31 A drive gear 65 is rotatably received in the accommodation space 33 and coupled to the drive shaft 61. The electric motor 60 rotates the rotating disk 40 at a minimum of 10 rpm to a maximum of 60 rpm. As the electric motor 60, a DC geared motor can be used. DC geared motor can operate based on strong force at initial driving even under load, and has the advantage of constant speed control.

The drive gear 65 is rotatably supported by the lower and upper covers 31 and 36. For example, support shafts 67 are formed at the lower and upper portions of the drive gear 65, respectively, and each support shaft 65 is inserted into the shaft insertion hole 69 formed at the lower and upper covers 31 and 36, respectively. do. The shaft insertion holes 69 are formed larger than the diameter of the support shaft 67. The support shaft formed in the lower portion of the drive gear is formed with a semi-circular coupling groove (not shown) to which the drive shaft 61 of the electric motor is coupled. The drive gear 65 meshes with the cog wheels of any one of the rotating disks 40. Accordingly, when the electric motor rotates, the drive gear 65 rotates, and as the drive gear 65 rotates, the rotating disks 40 rotate simultaneously.

The light source array module 50 is supported by a support frame 60 mounted in a module mounting space (not shown) formed at the rear side of the chamber 10. And the module mounting space is partitioned from the outside by the grill cover 63 is coupled to the rear side in the chamber (10). The grill cover 63 is formed with a plurality of vents (65) so that air can flow. A plurality of light source insertion holes 15 into which the light source mounted on the light source array module 50 is inserted are formed at the rear of the chamber 10.

In the illustrated example, two light source array modules are installed in the chamber. One light source array module 50 irradiates ultraviolet rays in a horizontal direction in the light irradiation space 11 of the chamber 10, and the other light source array module 50 is located in the light irradiation space 11 of the chamber 11. Irradiate UV light obliquely from the top to the bottom. This is to allow the ultraviolet light to be evenly distributed to the side and the top of the surface of the implant member 3.

Each light source array module 50 has a plurality of light emitting diode chips 53 arranged in a pattern set on the heat sink 43. A heat radiation compound is applied between the light emitting diode chip 53 and the heat sink 51 to effectively transfer the heat of the light emitting diode chip 53 to the heat sink 51. The light source applied to the light source array module 50 is a light emitting diode. Light irradiation by a light emitting diode (LED) does not generate much heat, can precisely control the wavelength, and has a feature of maintaining a wide beam. The light emitting diode applied to the present invention preferably irradiates ultraviolet rays having a 365 nm wavelength.

As the controller 70, a microcontroller may be used. The controller 70 sets the output of ultraviolet rays, the rotational speed, and the operation time of the motor desired by the user through the key input of the key control unit 27, and the set items are displayed on the display unit 29.

On the other hand, when the user accidentally opens the door 20 during light irradiation, in order to ensure stability to cut off the power at a high speed should be able to reduce the damage caused by ultraviolet rays. To this end, a sensor unit for detecting opening and closing of the door 20 is installed, and when the door 20 is opened by detecting a signal output from the sensor unit, the control unit 70 cuts off the power applied to the light source array module 50. At the same time, a warning sound is generated through the buzzer 75 installed in the base part. The limit switch 80 may be applied to the sensor unit. The limit switch 80 may be installed at the switch mounting portion 17 formed on the front surface of the chamber 10 as an example. When the door 20 is closed in the chamber 10, a connection bar (not shown) formed inside the door 20 is inserted into the switch mounting portion 17 to come into contact with the limit switch 80.

Hereinafter, a method for modifying a dental implant surface using an ultraviolet light irradiator will be described with reference to FIGS. 1 to 4.

In the dental implant surface modification method of the present invention, the implant member 1 having the titanium oxide film formed on the surface is first mounted on the table 30 installed in the light irradiation space 10 of the chamber 10. At this time, the implant member 3 may be mounted only on some of the rotating disks of the plurality of rotating disks 40, or all of the implant members may be mounted on each of the rotating disks.

After mounting the implant member 3 on the table 30, the door 20 is closed and the key operation unit 27 is operated to drive the electric motor. At this time, the user can arbitrarily adjust the rotational speed of the electric motor within 10 to 60rpm by the operation of the key control unit 27. As the electric motor is driven, the rotating disk 40 rotates, and thus the implant member 3 mounted on the rotating disk 40 also rotates together.

Next, power is applied to the light source array module 50 to irradiate the surface of the implant member 1 with ultraviolet rays. The user may set the output of the ultraviolet rays and the operation time through the key operation unit 27. The set value is displayed on the display unit 29. In the ultraviolet irradiation process, it is preferable to irradiate the implant member 3 with ultraviolet light for 3 to 24 hours at a light quantity of 2 mW / cm ² . Hydrophilicity and osteoadhesion are effectively increased within the above light quantity and irradiation time.

Experimental Example

In order to examine the bone adhesion promoting effect of the implant member using the ultraviolet light irradiator of the present invention, the hydrophilicity, toxicity to osteoblasts and the degree of proliferation were tested.

1. Specimen Fabrication

Disc-shaped specimens treated with RBM (Resorbable Blast Media) were fabricated in the same process as a conventional implant member. Specimens used were machined from titanium (CP Ti Gr4, DYNAMET A Carpenter Company, USA) in the form of discs with a diameter of 14 mm and a thickness of 3 mm on a precision CNC dedicated to implants (CINCOM, L20VIII, Japan), and then injected into a blasting machine. Apatite media was sprayed onto the surface of the disk for RBM treatment and then acid washed to remove residue.

The surface residues of the specimens subjected to the surface treatment as described above were analyzed by X-ray photoelectron spectrometer (XPS) (ESCALAB250, VG Scientific, U.K.) and the results are shown in FIG. 6.

Referring to the graph of FIG. 6, it can be seen that the surface of the disk subjected to the surface treatment is composed of C, O, N, and Ti elements. This means that the median apatite hydroxide (main component: Ca, P) was completely removed by the pickling process. In addition, when looking at the binding energy on the data peak, it can be seen that the Ti-2P phase and the O-1S phase form titanium oxide around 457 eV and 531 eV, respectively. This titanium oxide (TiO ₂ ) is in the form of a stable oxide, formed throughout the disk surface.

2. UV irradiation

Ultraviolet rays were irradiated onto the surface of the specimen using the light irradiator shown in FIG. 1. At this time, the specimen was placed on top of the rotating disk on which the locking rod was not formed and irradiated with ultraviolet rays. As an ultraviolet light source, a UV LED (Model: NCSU033A, 365 nm) manufactured by Nichia Corporation was used as a light emitting diode. The experiment was carried out by varying the irradiation time with light quantity 2mW / cm ² while rotating the specimen at a speed of about 35rpm.

3. Hydrophilicity Measurement

The surface hydrophilicity of the specimens was measured using the Sessile drop method. After dropping 0.025 ml of distilled water per specimen, take a picture using a video camera-equipped measuring device (Camscope®, Sometech, Korea) and use the Dropsnake® (National Institute of Health, USA) to determine the left and right contact angles in the picture. The average value was calculated | required after a measurement.

The control and the ultraviolet rays (365nm, 2mW / cm ² ) of the water droplets formed on the specimens irradiated for 3h, 6h, 12h, 18h, 24h, and a photograph showing the average contact angle is shown in Figs.

7 to 12, as the UV irradiation time increases, the average contact angle decreases. As shown in FIG. 7, the average contact angle of the specimen with the ultraviolet irradiation time of 0 hours, that is, the specimen not irradiated with ultraviolet rays (hereinafter, referred to as the control group) was 60 °. On the other hand, UV-radiated specimens (hereinafter referred to as test group) showed that the average contact angle was smaller than 60 °. In the case of the specimen surface irradiated for 18 hours, the average contact angle was about 19.43 °, indicating that the hydrophilicity was greatly increased. This seems to be the result of modifying the implant surface in the direction of enhanced hydrophilicity by Ti-OH induced by irradiation of ultraviolet rays to the titanium oxide film.

4. Cytotoxicity and Cell Proliferation Experiment

To determine whether the UV-irradiated implants were toxic to surrounding cells during implantation, the toxicity and proliferation of osteoblasts were checked.

As osteoblasts used in the experiment, MG63 (osteosarcoma, primary, human, Korea Cell Line Bank) cells were used. To culture the cells, DMEM (minimal) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA), 100 U / ml penicillin and 100 μg / ml streptomycin in a 100 mm diameter cell culture dish essential medium) (Gibco, Grand Island, NY, USA) was added to the incubator at 37 ℃, 100% humidity, 5% CO ₂ conditions. Cells were passaged when 70% confluent.

After each UV put one at (365nm, 2mW / cm ²⁾ for 6h, 12h, 18h, each culture dish in the irradiated specimen 24Well (corning Co., USA) for 24h dispensing the cell culture medium 5 × 10 ⁴ by 6 hours After 400 μl of 10% DMEM was added to each well, the cells were cultured for 1, 4, and 7 days in the cell incubator, and the toxicity and proliferation were confirmed by XTT assay.

200 μl of the cell culture solution was taken to measure absorbance at A450, and the basic absorbance values for the culture and the reagent were measured at A630 to correct the A450 value. The experimental results are shown in the following Tables 1 to 3 and the graphs of FIG. 13.

Psalter   UV irradiation time (hr) 0 6 12 18 24 A450

0.716 0.781 0.832 0.794 0.802 0.714 0.550 0.617 0.633 0.610 0.760 0.663 0.500 0.690 0.696 A630

0.067 0.068 0.069 0.072 0.071 0.061 0.06 0.064 0.064 0.062 0.065 0.07 0.066 0.067 0.067 A450-630

0.649 0.713 0.763 0.722 0.731 0.653 0.490 0.553 0.569 0.548 0.695 0.593 0.434 0.623 0.629 Average 0.666 0.599 0.583 0.638 0.636 Standard Deviation 0.025 0.112 0.167 0.078 0.092 100% conversion (%) 100% 89.9 87.5 95.7 95.4

Looking at the results of Table 1, in the absorbance after 1 day of culture, it appeared slightly lower than the control group when irradiated with UV rays for 6 hours and 12 hours, but a greater difference than the control group when irradiated with UV rays for 18 hours or more It can be seen that.

Psalter   UV irradiation time (hr) 0 6 12 18 24 A450

1.874 1.864 1.868 1.931 1.894 1.691 1.763 1.954 1.712 2.191 2.374 1.599 2.069 1.803 1.83 A630

0.07 0.068 0.072 0.072 0.069 0.066 0.07 0.07 0.071 0.066 0.07 0.074 0.073 0.073 0.077 A450-630

1.804 1.796 1.796 1.859 1.825 1.625 1.693 1.884 1.641 2.125 2.304 1.525 1.996 1.730 1.753 Average 1.911 1.671 1.892 1.743 1.901 Standard Deviation 0.352 0.137 0.100 0.110 0.197 100% conversion (%) 100% 87.4 99.0 91.2 99.4

Looking at the results of Table 2, after 4 days of culture, the absorbance was about 13% lower than that of the control group when irradiated with UV radiation for 6 hours, but no significant difference was observed when UV irradiation was performed for 12 hours or longer. Able to know.

Psalter   UV irradiation time (hr) 0 6 12 18 24 A450

1.948 1.974 2.077 2.014 1.995 2.047 1.89 1.932 1.911 2.058 1.928 1.948 1.796 1.996 1.985 A630

0.065 0.059 0.06 0.063 0.064 0.066 0.062 0.063 0.063 0.064 0.073 0.067 0.068 0.068 0.071 A450-630

1.883 1.915 2.017 1.951 1.931 1.981 1.828 1.869 1.848 1.994 1.855 1.881 1.728 1.928 1.914 Average 1.906 1.875 1.871 1.909 1.946 Standard Deviation 0.066 0.044 0.145 0.054 0.042 100% conversion (%) 100% 98.3 98.1 100.1 102.0

Looking at the results of Table 3, after 7 days of culture, the test group was not significantly different from the control group as a whole.

To summarize the results of Table 1 to Table 3, and Figure 13, the test group did not show a significant difference in cytotoxicity and proliferation compared to the control. By 7 days of cell culture, the cell viability was 95.3% (integral value of all values) compared to the control group, and less than about 87% of the cell numbers of 1, 4, and 7 days of each specimen were not found. In view of the above results, it is judged that irradiating the implant member with ultraviolet light does not significantly affect cytotoxicity and proliferation.

5. Cell Attachment Test

In order to determine the effect of the material on the adhesion reaction of the surrounding cells during the implantation of the implant member irradiated with UV light (UV), the degree of attachment of bone cells to the specimen was confirmed. For the experiment, put the specimens irradiated with ultraviolet rays (365 nm, 2 mW / cm ² ) for 6 h, 12 h, 18 h, and 24 h, respectively, into the 24Well corning Co., USA and dispense osteoblast culture medium by 5 × 10 ⁴ . After 6 hours, 400 μl of 10% DMEM was further treated in each well. After 6 hours, the culture medium was removed, cell pretreated for scanning electron microscopy, and cell adhesion was confirmed using the scanning electron microscope.

14 to 18 illustrate the control and 250 magnification images of osteoblasts attached to the specimens irradiated with ultraviolet rays for 6, 12, 18, and 24 hours. In Table 4 below, the number of cells attached to the cells in the image shown in FIGS. 14 to 18 was confirmed and quantified. Quantitative values of cell adhesion image five areas on the image (upper, lower, left, right, middle).

Psalter   UV irradiation time (hr) 0 6 12 18 24
Adherent cell number


Prize 88 120 125 111 99 Ha 131 125 131 125 122 Left 102 119 104 111 135 Right 110 125 111 102 122 medium 84 75 95 100 88 Average 103 112.8 113.2 109.8 113.2 Standard Deviation 18.8 21.3 14.8 9.9 19.1 100% conversion (%) 100.0 109.5 109.9 106.6 109.9

Overall, the test group was observed to be higher than the number of cells attached to the control group (average 108.9% adhesion rate compared to the control group).

19 to 23 illustrate a control and 1000-fold enlarged images of osteoblasts attached to the specimens irradiated with ultraviolet rays for 6, 12, 18, and 24 hours. As a result of 1,000-magnification image observation, cell-cell contact confirmed during cell proliferation and differentiation in UV-irradiated specimens.

In summary, the hydrophilic properties were found to improve the hydrophilicity significantly because the average contact angle of 60 ° can be reduced to less than 19.43 ° in the test group, it did not appear to have a significant effect on the cytotoxicity and proliferation. And in the experiments on the attachment reaction with the surrounding cells at the time of implantation in the body, the number of cell attachment was greater than that of the control group, and the cell-cell contact confirmed during the cell proliferation and differentiation.

Therefore, according to the present invention, by irradiating ultraviolet (Ultraviolet) to the implant member coated with a titanium oxide film or artificially formed titanium oxide film naturally on the surface, it was confirmed that the hydrophilic characteristics can be imparted to the implant member surface. In addition, the absence of a hydrophilic surface-modified implant may promote interaction with biological solutions, cells and tissues to enhance early osseointegration.

In the above, the present invention has been described with reference to the embodiments shown in the drawings, which are merely exemplary, and those skilled in the art will understand that various modifications and equivalent embodiments thereof are possible.

Therefore, the true scope of protection of the present invention should be defined only by the appended claims.

10: chamber 11: light irradiation space
20: door 30: table
31: lower cover 36: upper cover
40: rotating disk 41: gear
43: first disc part 45: second disc part
47: fixing rod 50: light source array module

Claims (6)

A chamber in which a light irradiation space is formed;
A door installed in the chamber to open and close the light irradiation space;
A table installed in the light irradiation space of the chamber and having an implant member having a titanium oxide film formed on a surface thereof;
A light source array module installed in the chamber and arranged in a pattern in which a plurality of light sources are installed to increase the hydrophilicity of the surface of the implant member by irradiating ultraviolet rays to the surface of the implant member;
And rotating means for rotating the implant member mounted to the table so that ultraviolet rays can be uniformly irradiated onto the surface of the implant member. The implanter of claim 2, wherein the light source of the light source array module is a light emitting diode having a 365 nm wavelength. The lower cover of claim 1 or 2, wherein the table is coupled to the chamber and has a receiving space formed therein, an upper portion of which is opened, and a plurality of first through holes formed at regular intervals, and an upper portion of the lower cover. Removably coupled to the upper cover and the second cover is formed in a position corresponding to each of the first through holes, a plurality is accommodated so as to be rotatable in the receiving space of the lower cover, one of which is rotated by the rotating means And rotating disks rotating simultaneously to the rest,
Each of the rotating disks is meshed with an adjacent rotating disk and formed to have a larger diameter than the diameter of the first and second through holes, and a first disc portion formed under the cog wheels and inserted into the first through holes. And a second disc portion formed on the cog wheel and inserted into the second through hole and mounted with the implant member, and formed in the second disc portion to prevent flow of the implant member during rotation of the second disc portion. Ultraviolet light irradiator for implants comprising a fixing means for preventing. According to claim 3, wherein the rotating means is installed in the base portion for supporting the lower portion of the chamber and the drive shaft is accommodated so as to be rotatable in the receiving space of the lower cover and the drive shaft and the drive shaft And a driving gear engaged with the cog wheel of any one of the rotating disks,
A sensor unit installed in the chamber to detect the opening and closing of the door and outputting a signal, and blocking the power applied to the light source array module when the door is opened by the output signal of the sensor unit, and at the same time, a warning sound through the buzzer Ultraviolet light irradiator for implants, characterized in that further comprising a control unit for generating. Mounting an implant member having a titanium oxide film formed on a surface thereof to a table installed in a light irradiation space of a chamber;
Rotating the implant member mounted to the table;
And irradiating the surface of the implant member with ultraviolet light through a light source installed in the chamber, thereby increasing the hydrophilicity of the surface of the implant member. The method of claim 5, wherein the light source is a light emitting diode having a 365nm wavelength,
The ultraviolet light is implanted surface modification method, characterized in that for irradiating for 3 to 24 hours at 2mW / cm ² to the implant member rotating at 10 to 60rpm.

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