WO2013100331A1

WO2013100331A1 - Manufacturing method for dental implant having hydrophilic surface for enhanced ossification

Info

Publication number: WO2013100331A1
Application number: PCT/KR2012/008018
Authority: WO
Inventors: 김수경; 강은정; 송주동; 엄태관; 최규옥
Original assignee: 오스템임플란트 주식회사
Priority date: 2011-12-27
Filing date: 2012-10-04
Publication date: 2013-07-04
Also published as: KR101196171B1

Abstract

The present invention relates to a manufacturing method for a dental implant having a hydrophilic surface for enhanced ossification in which the surface of an hydrophobized implant is hydrophilized and affinity thereof to bodily fluids and blood is improved by irradiating same with ultraviolet light, thus ultimately enhancing ossification after an implant procedure, and allowing a bone fusion period to be shortened.

Description

【Specification】

[Name of invention]

Manufacturing method of dental implant with hydrophilic surface with bone formation promoting effect

The present invention relates to a method for manufacturing a dental implant having a hydrophilic surface having an effect of promoting bone formation, and to irradiate the surface of the hydrophobized implant with ultraviolet rays to hydrophilize the surface and improve body fluid and blood affinity. The present invention relates to a method for producing a dental implant that can improve the bone formation effect and shorten the bone fusion period after the implant procedure.

Background Art

<2> In the case of surface treatment methods such as RBM and SLA, which are currently used in the implant surface treatment method, an oxide layer grows on the surface of the product when the product is exposed to air after the Table 3 treatment process as shown in FIG. Adsorption of various pollutants, such as hydrocarbons, occurs and becomes hydrophobic as it changes to a chemically stable state. Since the hydrophobized surface has low wettability to body fluids and blood, it has a disadvantage of prolonging the period of stabilization of the implant by preventing the overall process of fusion with bone after implant implantation.

In order to overcome the disadvantages caused by the hydrophobicity of the titanium surface, techniques for hydrophilizing the surface by coating a material such as a hydrophilic polymer polymer have been developed, but all of these methods change the shape of the surface by a predetermined level or more. There was a limit to providing hydrophilicity while maintaining the surface shape.

[Detailed Description of the Invention]

[Technical problem]

The purpose of the present invention is to solve problems of surface treatment methods such as RBM and SLA, to hydrophilize the hydrophobized surface and to improve the fluid and blood affinity of the implant. It is to provide a method for producing a dental implant having a hydrophilic surface that can improve the formation and shorten the period of bone fusion.

Technical Solution

<5> The present invention for achieving the above object is a step of forming a roughness on the dental implant surface of titanium or titanium alloy material, and irradiated with ultraviolet rays of 10 ~ 400nm wavelength on the surface of the implant is formed rough surface contamination source It provides a method for producing a dental implant having a hydrophilic surface comprising the step of oxidizing.

In this case, ultraviolet rays irradiated onto the surface of the implant to oxidize the surface contamination source It is preferable to have a wavelength of 100 to 200 nm to generate the reactive oxygen species, and it is preferable to irradiate ultraviolet rays of 320 to 400 nm having a photocatalytic effect. _, .

In addition, it is preferable to further include a step of accelerating the oxidation of the surface contaminant by volatilizing the surface contaminant by irradiating the surface of the dental implant oxidized surface of the dental implant 180 ~ 190nm ultraviolet rays.

Advantageous Effects

According to the present invention, the hydrophobized surface of the implant may be hydrophilized and the body fluid and blood affinity of the implant may be improved without finally undergoing a complicated process, thereby improving bone formation and shortening the bone fusion period after the implant procedure. .

[Brief Description of Drawings]

1 is a schematic view showing the hydrophilization process according to the present invention of the hydrophobized titanium implant surface.

2 and 3 are graph and photographic results showing surface contact angle changes with UV-ozone treatment time of SA disks.

<π> Figure 4 is the result of measuring the blood affinity of the dental implant treated with ultraviolet rays.

5 is a result of measuring the protein adhesion amount of the UV-treated dental implant.

6 is a result of measuring the cell adhesion amount of the UV-treated dental implants.

7 is a result of measuring the interfacial bone bonding force of the UV-treated, dental implant.

[Best form for implementation of the invention]

Hereinafter, a method for manufacturing a dental implant having a hydrophilic surface having a bone formation promoting effect according to an aspect of the present invention will be described in detail with reference to the accompanying drawings.

<16> The nature of the implant surface is an important factor in the bone adhesion process in which new bone tissue is fused to the implant surface, and the implant surface is changed to improve implant fixation and hardening of the implant to hard tissue. It can accelerate the healing process by strengthening the bone adhesion of the implant.

Osteoadhesion of such implants can be improved by creating micro-grained roughness on the implant surface, and the micro-grained roughness can be improved by particle spraying, absorbent spray media, acid etching, alkali etching, and titanium plasma. It can be formed by a variety of methods, such as spray method, particle treatment after acid treatment method, anodization method, laser surface processing method. Implants formed with micro roughness have the advantage of increasing the surface area and improving bone adhesion. However, as the surface area is large during storage before implantation, contamination caused by various pollutants in the air increases. There are disadvantages. In particular, the carbon pollutant present in the air is irreversibly adsorbed on the surface of the implant to hydrophobic the surface of the implant.

When the surface of the implant is contaminated and hydrophobized by the contaminants, various blood proteins do not adhere to the surface of the implant when the implant is implanted in vivo, and as a result, the biocompatibility of the surface of the implant is reduced and bone adhesion becomes difficult. In addition, inflammatory reactions may be caused by the pollutants.

The present invention is to form a roughness on the surface of the dental implant made of titanium or titanium alloy, in order to hydrophilize the titanium surface in which the various contaminants including hydrocarbons described above, 10 ~ 10 on the surface of the implant formed Ultraviolet rays having a wavelength of 400 nm may be irradiated and removed by oxidizing surface contamination sources.

That is, the present invention, as shown in Figure 1 by oxidizing and volatilizing the surface contamination source through the ultraviolet irradiation on the titanium surface in which various pollutants including hydrocarbons exist, by removing the contamination source causing surface hydrophobicity of the titanium surface The hydrophilicity of the surface improves body fluid and blood affinity, which ultimately improves bone formation and shortens bone fusion after implantation.

The ultraviolet rays irradiated onto the implant surface for the oxidation of the surface contaminants are preferably ultraviolet rays having a wavelength of 100 to 200 nm to generate reactive oxygen species, wherein the ultraviolet rays having 320 to 400 nm wavelength have a photocatalytic effect. Can be investigated together to promote oxidation of the pollutant.

In addition, in order to further accelerate the oxidation of the surface contaminant to volatilize the contaminant, the step may be additionally performed by irradiating 180-190 nm wavelength ultraviolet rays to the dental implant surface of which the surface contaminant is oxidized. The hydrophilized implant according to the present invention is preferably implanted during a period in which the contaminant is not resorbed on the hydrophilized surface.

Hereinafter, the effects of the present invention will be described in detail with reference to the following examples. However, these examples are provided to illustrate one or more embodiments by way of example, but the scope of the invention is not limited to these examples.

<25>

Example 1 Hydrophilic Titanium Surface Agent Removed Contaminants from Dental Implant Surfaces article

<27> The machined titanium implant or disk is blasted for 1 to 60 seconds at a blasting pressure of 1 to 10 atm using A1203 powder having a particle size of 1 麵 or less, and then the acid treatment method using a mixed acid aqueous solution is used. Macro & micro morphology was applied to the implant or disc surface. The acid-treated dental titanium implant or disc was ultrasonically cleaned with ethane for 30 minutes and with distilled water for 30 minutes and then dried.

<28> After the UV treatment at 150nm and 350nm wavelength for 5 minutes to the implant or disk after the SA (Sandblasting and Acid etching) process, and further irradiated with UV light of 185nm wavelength for 5 minutes to adsorb and stabilize the surface After removal, the prepared hydrophilic implants or discs were used in Examples 2 to 5 below.

<29>

Example 2 Measurement of Contact Angle and Surface Carbon Pollution Degree of UV Treated Impulent

In order to check the wettability of the UV-treated dental titanium disk produced in Example 1, distilled water 5 was dropped in the center of the disk and photographed with a digital camera from the side. The contact angle of was measured. In addition, the contact angle was measured for each UV irradiation time in order to confirm the effect of improving the wettability of the titanium disk according to the UV irradiation time. At this time, as a negative control was used a disk without removing the source.

As shown in FIG. 2, as the UV irradiation time increases, the hydrophilicity and wettability increase, so that the contact angle of the SA disk is lowered. As shown in FIG. 3, when the ultraviolet ray is irradiated for 5 minutes, It was confirmed that the contact angle was lowered from 97 ^° to 0 ^° and modified to a hydrophilic surface.

<33> In addition, to measure the carbon contamination of the surface of the ultraviolet treated dental disk,

Using XPS to measure the element content distributed on the titanium surface is shown in Table 1 below. As can be seen in the table below, the carbon content of the SA disk was about 35%, but the carbon content was reduced to about 24% upon UV irradiation.

<34> [Table 1]

Example 3 Blood Affinity and Blood Protein Part of UV-treated Dental Implants Implantation measurement experiment

After irradiating UV light to the dental implant prepared in Example 1, the micro-pig (mkropig) blood to support the implant about 3mm in order to check the blood affinity, and then the blood coming up the surface of the implant Blood affinity was confirmed by height. As a result, as shown in Figure 4, it was confirmed that the blood wettability was improved by the ultraviolet irradiation, through which the blood affinity of the implant surface was improved through the ultraviolet treatment.

In addition, in order to confirm the blood protein adhesion amount of the UV-treated dental implant prepared in Example 1, the implant was loaded with about 1 5 in 5¾ BSA (bovine serum albumin) solution or FBS (Fetal Bovine Serum) Afterwards, BSA or plasma proteins adhered to the surface were confirmed by BCA assay. At this time, the negative control group was used as the implant was not removed.

As a result of the experiment, as shown in FIG. 5, the adhesion of blood proteins on the surface of the UV-treated implants was increased by about 70% in 5¾ FBS and about 40% in BSA.

<39>

Example 4 Accumulation of Cell Adhesion of Ultraviolet Treated Titanium Disc

<41> the Example 1, the osteoblast cell line MG63 in order to measure the cell adhesion function of the produced disc seeding with 1X10 ^S eel ls / disk and then in one hour the cultured adhering to the disc surface with crystal violet assay Cells Quantification. At this time, the negative control group was used as an implant was not removed. ^■

As a result of the experiment, as shown in FIG. 6, it was confirmed that the cell adhesion increased by about 40% in the experimental group irradiated with UV when compared with SA, which is a negative control group.

<43>

[Example 5] Animal experiment for measuring bone interface binding force of the dental implant treated with UV

In order to confirm the implant-bone interface force of the dental implant prepared in Example 1, the implant is placed in a micropig tibia and the removal torque after 16 days of bone formation period (removal torque) The measurement was made. At this time, the negative control group was used as the implant was not removed.

As a result of the experiment, as shown in FIG. 7, the torsional removal force was improved by about 50% in the experimental group irradiated with ultraviolet rays compared to the negative control group SA, resulting in a strong implant-bone interface binding force. It was confirmed to disappear. The present invention is not limited to the above specific embodiments and descriptions, and various modifications can be made by those skilled in the art without departing from the spirit of the invention as claimed in the claims. It is possible for such modifications to fall within the protection scope of the present invention.

Claims

[Range of request]

[Claim 1]

Forming a roughener on the surface of the dental implant made of titanium or titanium alloy; And

Oxidizing a surface contaminant by irradiating UV light having a wavelength of 10 to 400 nm to an implant surface having the roughening machine;

Method for producing a dental implant with a hydrophilic surface allol comprising a.

[Claim 2]

The method of claim 1,

Method for producing a dental implant having a hydrophilic surface of the ultraviolet ray of 100 ~ 200nm wavelength to generate reactive oxygen species (Reactive Oxygen Species) is irradiated to the implant surface for oxidation of the surface contamination source.

[Claim 3]

The method of claim 2,

Method for producing a dental implant having a hydrophilic surface comprising a ultraviolet ray of 320 ~ 400nm wavelength that the ultraviolet rays irradiated to the implant surface for the oxidation of the surface contamination source has a photocatalytic effect.

[Claim 4]

The method of claim 1,

The surface of the dental implant of the dental implant having a hydrophilic surface further comprising the step of volatilizing the surface contamination by irradiating ultraviolet rays of 180 ~ 190nm wavelength on the surface of the dental implant oxidized surface Manufacturing method.