KR20110125958A - Composition for lowering cholesterol in blood containing the ethanol extract of cynanchum wilfordii as effective component - Google Patents

Abstract

PURPOSE: A pharmaceutical composition containing Cynanchum wilfordii extract for reducing blood cholesterol is provided to prevent and treat hypercholesterolemia. CONSTITUTION: A pharmaceutical composition for reducing blood cholesterol contains 0.02-50 wt% of Cynanchum wilfordii ethanol extract as an active ingredient. The ethanol is 70-90% ethanol. The Cynanchum wilfordii ethanol extract is prepared by washing, drying, and pulverizing Cynanchum wilfordii and extracting at 90-98°C for five to seven hours, and concentrating. The pharmaceutical composition is manufactured in the form of powder, granules, tablets, capsules, suspension, emulsion, syrup, external use formulation, and suppository.

Description

Composition for lowering cholesterol in blood containing the ethanol extract of Cynanchum wilfordii as effective component}

The present invention relates to a pharmaceutical composition for lowering cholesterol in the blood containing baekhsiao ethanol extract as an active ingredient. More specifically, the present invention provides a cortical artery disease by developing baekhsiao ethanol extract, which is a functional material capable of lowering blood cholesterol content. It can provide a very useful invention in the health food industry for the prevention and improvement of hypercholesterolemia inducing adult diseases such as heart disease, stroke.

Sewage is largely divided into Red sewage, which belongs to Nodeulaceae, and White sewage, which belongs to Park Juka. Drip-off leaves are alternate, narrow egg-shaped or heart-shaped, and the tips of the leaves cover the stem with the membrane. Flowers are small, many, dense, and hang on conical inflorescences with several branches, with 2-4 small flowers in bloom or several again, flowers are greenish white or white, about 6-15cm long, and the thick part is 3 diameters. It is ~ 12cm, the surface is reddish brown or purple, with uneven vertical grooves, rugged, and traces of roots at each end. Zhoushan is distributed in China's Hanam, Hubei, Guizhou, Sichuan, Jiangsu and Guangxi, and also in Zhejiang, Anjing, Guangdong, Shandong, Gangseo and Hunan. It has long been known to be effective in replenishing the liver, gods, nourishing blood, and removing wind, and whitening of the heads early. Efficacy in subdivision, chronic malaria, chronic diarrhea, chronic hepatitis, carbuncle, nausea, intestinal tract, hemorrhoids. White sewage is also conical, 5-10 cm long and 15-35 mm in diameter. The outer surface is grayish yellow to yellowish brown with many vertical wrinkles and hard quality. The cross section of this herb is white, and when viewed under the microscope, three to five layers of stone cell rings under the cork layer are broken.

On the other hand, 95% of the fat in human food is trigriceride, and the rest is cholesterol and phospholipids. Cholesterol is a major component of cell membranes and lipoproteins, and is a precursor of bile acids, steroid hormones, and vitamin D3. It is synthesized in most tissues except brain tissue and occurs most actively in the liver.

In adults, the amount of cholesterol synthesized in the body per day is about 9 to 11 mg / kg of body weight, which is much higher than the amount supplied to meals. do. Diseases related to cholesterol metabolism are mainly caused by the accumulation of cholesterol in the blood and tissues. Fats consumed through food are absorbed mainly in the duodenum and jejunum, and these fats are gradually absorbed by the emulsification of bile salts secreted from the liver before absorption. It is changed to a small fat spot. They are then degraded in the form of free fatty acids, monoacylglycerols or diacylglycerols, free cholesterol and lysophosphatidyl choline by the action of lipase secreted from the pancreas. Among them, water-soluble glycerol and lysophosphatidyl choline are easily absorbed, but the remainder is dissolved in hydrophobic and hydrophilic mixed micelles and then absorbed. These colloids consist mainly of monoacylglycerols, fatty acids, bile salts and small amounts of cholesterol, and the role of bile acids is essential for their formation. Lipids absorbed in the form of colloidal particles are esterified in enterocytes and converted into triglyceride and esterified cholesterol and then combined with apolipoprotein to form chylomicrons. do. In this process, cholesterol that is not dissolved into the colloid is excreted through the intestine.

Hypercholesterolemia is well known as a risk factor for coronary artery disease. The purpose of the prevention and treatment of hypercholesterolemia is to remove excess cholesterol through normal lipid metabolism in the body. According to research reports of developed countries, cerebrovascular and cardiac diseases, which account for more than 30% of all mortality rates, are vascular diseases represented by cerebral hemorrhage, cerebral thrombosis, heart failure, and myocardial infarction. It is actively underway.

The present invention is derived from the above requirements, the present invention, but the development of a functional material that can lower the blood cholesterol content is desired, these days being a lot of restrictions due to safety problems such as side effects, natural products and relatively safe The present invention was completed by developing an extract of ethanol extract of Baeksuohao, which is a herbal material that has been used based on this high and long experience.

In order to solve the above problems, the present invention is Baek Ha Shou ( Cynanchum wilfordii ) provides a pharmaceutical composition for lowering cholesterol in the blood containing ethanol extract.

In addition, the present invention is a white ethanol extract obtained by the method of washing, drying and crushing the white sewage, followed by extracting for 5 to 7 hours at 90-98 ° C. with an ultrafast vacuum cold extractor by adding 70-90% ethanol. It provides a method of manufacturing.

In addition, the present invention provides a functional food having blood cholesterol lowering activity, including baekhsiao ethanol extract.

According to the present invention, the Baek Hao Shou ethanol extract of the present invention has an excellent effect of lowering lipids in blood and liver tissue, thereby preventing and improving hypercholesterolemia inducing adult diseases such as coronary artery disease, heart disease and stroke. It is a very useful invention in the food industry.

1 is a result showing the serum glucose content of male (A) and female (B) mice according to the ingestion of Baek Sewage. I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet), bars represent mean ± standard error. n represents 5 rats per group. Other letters indicate significant other values evaluated by Duncan's multiple test ( P <0.05).
Figure 2 is a result showing the serum high-density cholesterol (HDL-cholesterol) content of male (A) and female (B) mice according to the ingestion of Baek Sewage. I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet), bars represent mean ± standard error. n represents 5 rats per group. Other letters indicate significant other values evaluated by Duncan's multiple test ( P <0.05).
Figure 3 shows the results of serum triglyceride content of male (A) and female (B) rats according to the ingestion of Baek Sewage. I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet), bars represent mean ± standard error. n represents 5 rats per group. Other letters indicate significant other values evaluated by Duncan's multiple test ( P <0.05).
4 is a diagram showing the results of histochemical analysis of livers of SD male rats following ingestion of Baek Sewao. (A) I (Control), (B) II (1% Cholesterol Control), (C) V (1% Cholesterol and 0.5% White Sewage), (D) VI (1% Cholesterol and 1% White Sewage).

In order to achieve the object of the present invention, the present invention provides a pharmaceutical composition for lowering blood cholesterol, comprising ethanol extract of Cactenum wilfordii.

In the pharmaceutical composition for lowering cholesterol in the blood according to one embodiment of the present invention, the Baeksewao ethanol extract is preferably 70 to 90% ethanol extract, more preferably 80% ethanol extract.

In the pharmaceutical composition for lowering cholesterol in the blood according to one embodiment of the present invention, the Baek Sewage ethanol extract is washed, dried and crushed Baew Sewage, and 70 to 90% ethanol is added at 5 to 90 to 98 ° C. with an ultra-fast vacuum cold extractor. It can be obtained by the method of extracting for ~ 7 hours and then concentrating, preferably washing the white sewage, drying and pulverizing, adding 80% ethanol and extracting at 95 ° C. for 6 hours using an ultrafast vacuum cold extractor and then concentrating. Can be obtained by

The pharmaceutical composition for lowering blood cholesterol of the present invention may include 0.02 to 50% by weight of the extract based on the total weight of the composition.

Pharmaceutical compositions comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage forms of the extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.

Pharmaceutical compositions comprising extracts according to the invention, in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. Can be formulated and used. Carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate And various compounds or mixtures including cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil and the like. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, and such solid preparations may include at least one excipient such as starch, calcium carbonate and sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 100 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The extract of the present invention can be administered to mammals such as rats, mice, livestock, humans and the like in various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

In addition, the present invention is a white ethanol extract obtained by the method of washing, drying and crushing the white sewage, followed by extracting for 5 to 7 hours at 90-98 ° C. with an ultrafast vacuum cold extractor by adding 70-90% ethanol. It provides a method of manufacturing. Preferably, the white sewage is washed, dried and pulverized, and 80% ethanol is added, followed by extraction at 95 ° C. for 6 hours using an ultrafast vacuum cold extractor, followed by concentration.

In addition, the present invention provides a functional food having blood cholesterol lowering activity, including baekhsiao ethanol extract.

In the functional food having blood cholesterol lowering activity according to one embodiment of the present invention, the Baekshou ethanol extract is preferably 70 to 90% ethanol extract, more preferably 80% ethanol extract.

In addition, the present invention provides a health functional food comprising a baekhsiao ethanol extract and a food supplement acceptable food supplements exhibiting a blood cholesterol lowering effect. Examples of the food to which the extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

It may also be added to foods or beverages for the purpose of lowering cholesterol in the blood. At this time, the amount of the extract in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5 g, preferably 0.3 to 1g based on 100 ml. have.

The health functional beverage composition of the present invention has no particular limitation on the other ingredients other than the above-mentioned extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract, for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. . The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the extracts of the present invention may contain flesh for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components may be used independently or in combination, and the proportion of such additives is not so critical but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Way

The purpose of this study was to analyze the nutritional value of sewage by comparing the effect of supplementation with Baek Sewage on blood cholesterol levels in male and female rats.

1. Experimental material

In this experiment, Baek Ha- seo Baekryong-do ( Cynanchum wilfordii ) was purchased and used. The sample was washed, dried and pulverized, and 80% ethanol was added thereto, followed by extraction for 6 hours at 95 ° C. using a COSMOS-660 ultrafast vacuum cold extractor, followed by concentration.

2. Breeding and Experimental Diet of Experimental Animals

Seventy-week-old female Sprague-Dawley strains of 250g-260g female rats and 50 330-350g male Sprague-Dawley strains were distributed from Orient Bio Co., Ltd., and were freely supplied with solid feed and water. After one week of adaptation, the male and female were divided into six groups of five males according to the randomized block design, and the experimental diet was supplied for five weeks. The experimental group consisted of the normal diet control group (I), cholesterol control group (II), 0.5% white sewage supplement group (III), 1% white sewage supplement group (IV), and cholesterol-0.5% white sewage supplement group (Ⅴ). ), And divided into cholesterol-1% Baek Sewao supplement group (VI) (Table 1).

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Dietary (10 g / kg of diet)

The diet used in the experiment was prepared by modifying the dietary composition of AIN-93M based on the dietary requirements of adult rats in 1982 by the American Institute of Nutrition (AIN). The amount of cholesterol added to the cholesterol group was 1% of dietary weight, and the amount of white sewage was added 0.5% and 1% of dietary weight, respectively. Water and diet were supplied unrestricted, and the room temperature was 22 ± 0.5 ° C, relative humidity was 55 ± 5%, and the contrast was kept constant at 12 hour cycle (8 AM to 8 PM). Body weight and dietary intake were measured at regular times once a week to calculate dietary efficiency. The food efficiency ratio (FER) was calculated by dividing the weekly weight gain by the amount of food intake consumed during the same period.

3. Collection of blood and various organs

Blood was collected from the orbital artery, which was fasted 12 hours before the end of the experiment, and centrifuged at 3,500 rpm for 15 minutes (desk top centrifuge, Hanikl Sci. Ind., Seoul, Korea). Frozen at ℃. Immediately after blood collection, the experimental animals were sacrificed by cervical dislocation, and livers were extracted, washed with physiological saline, and water was removed with filter paper. Part of the liver was taken and stored in formalin solution for photography of the liver.

4. Blood analysis

Serum triglyceride (TG) analysis was performed on triglycerides reagents kit, LDL-cholesterol on LDL-cholesterol kit, HDL-cholesterol on Direct HDL-Cholesterol kit, and total cholesterol (TC) on triglyceride reagent kit (Bayer, USA) The blood biochemical test was analyzed with an automated analyzer ADVIA 1650 (Bayer, Japan), and total lipids were analyzed with an Agilent 8453 photometer (Agilent, Germany) using a total lipid reagent kit (Medicos, USA). It was.

5. Histological Observation of Liver

Liver tissues stored in formalin were fixed in 10% formalin to prepare paraffin blocks, mounted on slide glass, and subjected to H & E staining. Olympus BX51TF optical microscopy was observed 100 times and photographed.

6. Statistical Processing

All the results of the present invention were obtained using the SPSS 12.0 for windows package to obtain the mean and standard error of each group, the significance of the mean among the experimental group was carried out one-way analysis of variance (ONE-ANOVA) test, the significance test between each experimental group Duncan's multiple test was performed at the significance level p <0.05.

Example  One. Baek Ha Shou  Weight gain in rats, Dietary Intake  And Dietary efficiency

1.1 male

The initial body weights were all started without significant difference, but the final body weight was partially reduced in the supplement of ethanol extract. In particular, body weight was significantly decreased in 1% supplement (IV), 1% cholesterol and 1% baekshou (VI). Dietary supplementation The total weight gain for 5 weeks showed a tendency to decrease compared to the control in some of the ethanol extract supplemented group, especially in both cholesterol and ethanol extract supplemented group (Table 2). This suggests that the appetite suppressant component is inherent in the ethanol extract of Baek Hao Sew due to a decrease in body weight due to a decrease in dietary intake.

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

1.2 female

The initial body weights were all started without significant difference, but the final body weight was decreased in the supplemental diet of ethanol extract. In particular, the body weight was significantly decreased in 0.5% Baekshao Ethanol Extract Supplement (III), 1% Baeksiao Ethanol Extract Supplement (IV), 1% Cholesterol and 1% Baekshui (VI). Dietary supplementation The total weight gain for 5 weeks showed a tendency to decrease compared to the control group in both baekshou ethanol extract supplementation group, and significantly decreased in both cholesterol and baekshou ethanol extract supplementation (Table 3). This is thought to be a loss of body weight with a decrease in dietary intake. This suggests that the appetite suppressant component is inherent in the ethanol extract of Baek Hao Sew due to a decrease in body weight due to a decrease in dietary intake.

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

Example  2. Baek Ha Shou  Changes in Organ Weight in Rats with Intake

2.1 male

The liver weight of the cholesterol control group (II) was significantly increased compared to the control group (I), and liver hypertrophy due to supplementation of cholesterol was confirmed (Table 4).

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

2.2 Female

The liver weight of the cholesterol control group (II) was significantly increased compared to the control group (I), confirming hepatomegaly due to supplementation of cholesterol (Table 5). It was confirmed that the liver weight was significantly decreased in both the cholesterol and Baewha ethanol extract supplementation groups (Ⅴ, Ⅵ) compared to the cholesterol control group (Ⅱ).

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

Example  3. Baek Ha Shou  Rat intake In serum aspirate aminotransperase  (AST) and alanine aminotransferase  ( ALT ) 'S activity

3.1 male

It was confirmed that the AST activity of the cholesterol control group (II) significantly increased, and the supplementation of cholesterol increased the liver number (Table 6). Some AST activity was decreased in the supplement of baekshou ethanol extract. In particular, the 1% Baek Sewha Ethanol extract supplement (IV) was significantly decreased.

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

3.2 Female

It was confirmed that the AST activity of the cholesterol control group (II) significantly increased, and the supplementation of cholesterol increased the liver number (Table 7). The AST activity was decreased in all supplements of Baeksiao ethanol extract. In particular, 1% cholesterol and Baeksiao ethanol extract supplements (V, VI) were significantly decreased. The activity of ALT also showed the same tendency as AST, and the activity of ALT was decreased in the ethanol extract supplemented with Baek sewage.

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

Example  4. Baek Ha Shou  Effect of Serum Glucose Content and Lipid Pattern on Rats

4.1 Baek Ha Shou  Changes in Blood Glucose Content of Rats with Intake

4.1.1 Male

Glucose levels showed a tendency to decrease blood sugar levels in all supplemental ethanol extracts of Baeksiao OH. In particular, the hypoglycemic amount of 0.5% Baeksiao ethanol extract supplement (III), 1% cholesterol and 0.5% Baeksiao ethanol extract supplement (VI) was shown to have a significant effect on hypoglycemic effect on Baekshao ethanol extract ( 1A).

4.1.2 Female

Glucose levels tended to decrease in the ethanol extract supplemented with Baekshao ethanol compared to control (Ⅰ, Ⅱ). In particular, 1% cholesterol and 1% Baekshao supplement (VI) blood glucose reduction was significantly higher than that of the control (II), it was confirmed that there is a hypoglycemic effect on the ethanol extract of Baeksiao (Fig. 1B).

4.2 Baek Ha Shou  Serum Serum According to Intake HDL Cholesterol content change

4.2.1 Male

The content of HDL-cholesterol in the cholesterol supplementation group was decreased compared to the control group (I). HDL-cholesterol content was increased in both ethanol extract supplemented with Baeksiao, especially in 1% Baeksiao supplement (IV), 1% cholesterol and 1% Baekshou supplement (VI) compared to the control. Significantly increased the blood lipid improvement effect of the ethanol extract of Baek Sewao (Fig. 2A).

4.2.2 Female

The content of HDL-cholesterol in the cholesterol supplementation group was decreased compared to the control group (I). The content of HDL-cholesterol increased significantly in all supplements of Baeksiao ethanol extract, and the amount of HDL-cholesterol in the blood increased as compared with the control group. Lipid improvement effect was confirmed (FIG. 2B).

4.3 Baek Ha Shou  Changes in Triglyceride Contents of Rats According to Intake

4.3.1 Male

Compared with the control group (I), the content of triglycerides in the blood of the ethanol extract supplemented with Baeksiao was significantly reduced, and the supplementation of Baekshou ethanol extract was confirmed to reduce the concentration of triglycerides in the blood (FIG. 3A).

4.3.2 Female

Compared with the control group (I), the content of triglycerides in the blood of the ethanol extract supplemented by Baeksiao was significantly reduced, and the supplemental supply of the baekshou ethanol extract was confirmed to reduce the concentration of triglycerides in the blood (Fig. 3B).

4.4 Baek Ha Shou  Changes in Serum Glucose Levels and Lipid Patterns of Rats with Intake

4.4.1 male

It was confirmed that hypercholesterolemia was induced by the cholesterol supplementation diet by increasing the total cholesterol concentration in the blood control group (II) (Table 8). Some total ethanol extract supplements showed a decrease in total cholesterol concentration in blood. The concentration of LDL-cholesterol was significantly increased in the cholesterol control (II). The total lipid content in blood tended to decrease in Baekshao ethanol extract supplements (III, IV) compared to the control (I).

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

4.4.2 Female

The concentration of LDL-cholesterol was increased in the cholesterol control group (II), and the trend was decreased in both the baekshou ethanol extract supplements (Table 9). In particular, there was a significant decrease in 1% cholesterol and 1% Baekshou supplement (VI). The total lipid content in blood did not show a significant difference in all groups.

I: control diet, II: cholesterol control diet (1%, 10 g / kg of diet), III: experimental diet containing 0.5% Baek Sewage extract (5 g / kg of diet), IV: containing 1% Baek Shou extract Experimental diet comprising (10g / kg of diet), V: 1% cholesterol and 0.5% Baekshou extract, Experimental diet (5g / kg of diet), VI: Experiment comprising 1% cholesterol and 1% Baekshou extract Diet (10 g / kg of diet). All data represent mean ± standard error. n represents 5 rats per group. Different letters in the column represent significant other values evaluated by Duncan's multiple test ( P <0.05). NS (not significant).

4.5 Baek Ha Shou  Rat intake Hepatic  Fat accumulation change

As a result of taking a picture of the liver tissue of the male rats, it was confirmed that the fat was significantly accumulated in the cholesterol control (FIG. 4B) compared to the control (FIG. 4A). Hepatic fat accumulation was significantly reduced in the 1% Cholesterol and Baeksiao Ethanol Extract Supplement (FIG. 4C, D), suggesting that the supplemental supply of Baeksiao Ethanol Extract is related to hepatic fat metabolism.

Claims (6)

Cynanchum wilfordii ) A pharmaceutical composition for lowering cholesterol in the blood containing ethanol extract as an active ingredient. The pharmaceutical composition of claim 1, wherein the ethanol is 70 to 90% ethanol. The method of claim 1, wherein the Baek Sewage ethanol extract is washed, dried and crushed Baek Sewage, and 70 to 90% ethanol is added and extracted by ultrafast vacuum low temperature extractor at 90 to 98 ° C. for 5 to 7 hours and then concentrated. Pharmaceutical composition for lowering blood cholesterol, characterized in that obtained by. Washing, drying and grinding the white sewage, 70 ~ 90% ethanol was added by ultrafast vacuum low temperature extractor for 5 to 7 hours at 90 ~ 98 ℃ extract, characterized in that obtained by the method of concentrating Manufacturing method. A functional food having blood cholesterol lowering activity, which contains baekhshao ethanol extract as an active ingredient. The functional food according to claim 5, wherein the ethanol is 70 to 90% ethanol.

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