KR20110125116A - Composition for the prevention and treatment of apotic dermatitis containing the mixed extract of chrysanthemum indicum linne. and steamed rheum undulatum with alcohol as an effective ingredient - Google Patents

Abstract

PURPOSE: A composition containing extract of Chrysanthemum indicum Linne. and Rheum undulatum is provided to reduce cytokine secretion in atopic skin and to relieve atopic dermatitis symptom. CONSTITUTION: A pharmaceutical composition for preventing and treating atopic dermatitis contains mixture extract of Chrysanthemum indicum Linne. and Rheum undulatum as an active ingredient. A method for preparing Rheum undulatum comprises: a step of mixing Rhei Rhizoma pieces and Oryza sativa fruit wine; a step of steaming the mixture. The mixture extract is isolate using 1,3-butyelen glycol. A neutraceutical food for preventing and treating atopic dermatitis contains the mixture extract as an active ingredient. An external use skin formulation or cosmetic composition for preventing and treating atopic dermatitis contains the mixture extract as an active ingredient. The mixture extract is formed in a nanoliposome.

Description

Composition for the prevention and treatment of apotic dermatitis containing the mixed extract of Chrysanthemum indicum Linne. and steamed Rheum undulatum with alcohol as an effective ingredient}

The present invention relates to an atopy prevention and treatment agent, atopy prevention and improvement health food, external skin preparation and cosmetic composition.

Atopic dermatitis is a recurrent chronic dermatitis with severe itching. About 10 to 15 percent of children have atopic dermatitis, and 90 percent report a spontaneous improvement within 5 years. Such atopic dermatitis generally improves in adulthood, so that about 30 to 40% of dermatitis does not reappear in appearance, but the rest are known to suffer from dermatitis, such as dry skin, irritation of skin irritants, housewives and eczema. In addition, in the case of sensitive skin, moisture retention and its recovery power are low, and skin keratinization, itching, and the like are likely to occur, so it is highly likely to become atopic skin.

The exact pathophysiology of atopic dermatitis is not yet fully understood, but the genetic predisposition is associated with immunological and non-immunological mechanisms. Exogenous atopic dermatitis, which accounts for the majority of atopic dermatitis, is caused by an immune mechanism associated with IgE, and there are many reports that a delayed immune response by T cell abnormality is involved rather than an immediate immune response to a particular allergen. Recently, Th2 related cytokines such as IL-4, which induce IgE production from B cells, have been reported to cause atopy (JS Kang et al., Ihhibition of atopic dermatitis by topical application of silymarin in NC / Nga mice.intl immunopharm. (2008) 8. 1475-1480).

In order to treat such atopy, conventional components such as ceramide, linoleic acid, vegetable oil or mineral oil, steroid preparations such as hydrocortisone or substances that have enhanced antibacterial and anti-inflammatory functions, DNA synthesis through UV therapy Inhibitors, cell hyperproliferation inhibitors, inflammation and itching inhibitors, and the like have been proposed. However, the steroidal agent may cause adverse effects such as growth inhibition or side effects of the epidermis, and urea peroxide may cause excessive irritation of the skin, and antibiotics such as antihistamines may be resistant to bacteria and photosensitive. There is a problem that is likely to cause side effects. In addition, when applied to the skin for a long time, there is a risk of causing serious side effects, such as capillary dilator and / or increase in the thickness of the stratum corneum, expansion, etc. Recently, gamma-linoleic acid that is widely used to relieve atopy (Korean Patent Publication No. 2000-0046633) ) Is easily oxidized, and inferior in stability, and relatively strong in skin irritation, making it difficult to use for sensitive skin.

Recently, the focus is on the search for natural substances that have an effect on atopy. Representatively, extracts of mugwort extract (Korean Patent Registration No. 10-0377262), Ganoderma Lucidum Mushroom, Yu Geun-pi, Licorice, Baekbong-ryeong, Baekjima and Baeknyeoncho (Korean Patent Registration No. 10-0517465), Evening Primrose Oil, Aloe, Wood Vinegar, Violet, Jujube, Matsutake mushroom, Aralia, Ginseng, Green tea, Tofu, Bokbunja, Schisandra chinensis, Mugwort, Pogongyoung, Sambaekcho, Astragalus, Hagocho, Haesong, Golden and dew extract (Korean patent registration No. 10-0451444), Celadon leaf and deficit Extracts of leaflets (Korean Patent Registration No. 10-0454752), extracts of dry yield, Euiin, Schisandra chinensis, Gilgyeong, Nabokja, Macmun-dong and Seokchangpo (Korea Patent Registration No. 10-0483539), lavender, eucalyptus and tea tree oil (Korean patent) Registration No. 10-0597997) and the like, in addition to the above life, haphwanpi, changja, malt and the like are known to be effective. However, the natural substances have a disadvantage in that their use is limited because their efficacy is insignificant or allergic to hypersensitivity in sensitive skin.

Therefore, there is a demand for developing a material that is more effective and safer than conventionally known atopy prevention and treatment agents.

Rheum undulatum is a dicotyledonous plant that is a perennial herb of the bark, and is native to China's West and Qinghai, and grows in valley wetlands. It has thick yellow roots, and the straight stem grows up to 1m in height. The inside is hollow, the leaf shape is a wide egg-shaped round shape, and the leaf body length is 25-30cm. Leaf veins on the back are clear, rarely dull teeth on the edges, and blunt ends. Flowers blossom in July-August, abdominal inflorescences form conical inflorescences at the ends of branches and stems, and yellow-white flowers with sacks are returned from flower heads. There are no petals and there are 9 stamens and 3 pistils. The basement is a vertically rooted root with many burdock roots. Fruits are capsules, triangular, with wings, and ripen in August. Used as a medicine is a one-root root, when grown in the fourth year, the root weight is 3 ~ 5kg. After removing 6 ~ 7 years old root roots and burdock-shaped cup roots, they are sliced and dried as it is called rhubarb. The main ingredient is an anthraquinone derivative, its glycosides, and tannins, and when ingested in small amounts, it exhibits a healthy effect (健 다 作用) .In large amounts, it is a laxative (緩 下 劑), which is good for constant constipation or indigestion. It is raw and strong in effect, and when you use ripe one, the effect is gentle, and the charcoal is strong in hemostasis.

Rhubarb is used raw, or liquor (酒炒), jujugi [酒蒸] is used, Juju Rhubarb refers to the steamed rhubarb. Rhubarb is highly volatile, so it's a rule to put it in late rather than as long as it is. This main rhubarb is known to have a stronger blood circulation effect than rhubarb.

Compositae plants are native plants throughout our country and are widely used for landscape composition and food ingredients. The countries that use chrysanthemums are China, Japan, and Korea, and the ornamental chrysanthemums are used for tea and herbal medicine, but many varieties have strong odor and bitter taste. I am doing it.

Chrysanthemum indicum Linne. Is a perennial herb that belongs to the Asteraceae family and is a wild chrysanthemum widely distributed in the sub-central mountains of our country. It is used as a herbal or private prescription to relieve Sogangsim, central nervous system, lowering blood pressure, anemia, dizziness and headache. In addition, the anti-cancer effect, the aldose reductase inhibitory effect, and the nitric oxide production inhibitory effect have been reported as functionalities of the components isolated from the gamguk. In addition, folk remedies have been used for windy signs, dizziness and pain in the liver, redness and tearing of the eyes due to fever, coughing, joint pain and high blood pressure, and chrysanthemum to clear the head and brighten the eyes. It is known to help liver lack of energy, to make eyes and blood vivid, to remove eye diseases, to make bloody and to lighten the body.

The biggest problem with atopic dermatitis is the abnormal activation of immune function, which leads to an increased inflammatory response, leading to increased cytokine production. Ultimately, to alleviate the symptoms of atopic dermatitis, it is necessary to search for natural drugs that can reduce inflammation and prevent overgrowth of the epidermis while maintaining moisturizing. Recent studies have shown that proteinase-activated receptor-2 (PAR-2) is closely associated with atopy development, searching for inhibitors and inhibiting the mechanism of NF-kB. It is time to search for substances that inhibit the mechanism of PAR2-> NF-kB and develop them as therapeutic agents for atopic dermatitis. In addition, the drug used in the treatment of skin diseases is good, but in fact, external preparations are effective. Especially, atopic dermatitis has severe itching caused by dry skin, so it is developed as an external preparation that can remove inflammation by fundamentally removing the factors causing itching and atopic dermatitis along with moisturizing. Is expected to be more effective.

Accordingly, the present inventors have found that the mixed extract of chrysanthemum and syrup, which significantly reduces the secretion of cytokines in atopic dermatitis-derived skin cells compared to the individual extracts of chrysanthemum and syrup, reduces the expression of cytokines and adhesion molecules. In addition to a significant improvement in clinical atopic symptoms in the atopic animal model, and significantly reduced the concentration of IgE in the blood functioning immune function, the mixed extract of chrysanthemum and major rhubarb of the present invention prevents and treats atopic dermatitis. The present invention has been completed by revealing that the composition can be used as a composition for improvement, a health food for improvement, an external preparation for skin, or a cosmetic composition.

It is an object of the present invention to provide a composition for preventing and treating atopic dermatitis, improving health food, improving skin external preparation, and cosmetic composition containing a mixed extract of chrysanthemum and syrup.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention and treatment of atopy containing a mixed extract of chrysanthemum ( Chrysanthemum indicum Linne.) And main rhubarb as an active ingredient.

In addition, the present invention provides a dietary supplement for atopic prevention and improvement containing a mixed extract of chrysanthemum and main rhubarb as an active ingredient.

In another aspect, the present invention provides a topical skin for preventing and improving atopy containing a mixed extract of chrysanthemum and main rhubarb as an active ingredient.

In addition, the present invention provides a cosmetic composition for preventing and improving atopy, which contains a mixed extract of chrysanthemum and main rhubarb as an active ingredient.

The mixed extract of chrysanthemum and symptom rhubarb of the present invention is a natural-derived extract with little cytotoxicity and is safe, and significantly improves atopic dermatitis by inhibiting abnormal activation of NF-kB and immune function that are activated as atopic dermatitis intensifies. Since it shows an effect, it can be usefully used as a composition for preventing and treating atopic dermatitis, improving health food, improving skin external preparation, and cosmetic composition.

1 is a graphical representation of the anti-atopic efficacy of extracts in an atopic animal model compared to clinical skin severity scores:
Different alphabets of a, b, c indicate a statistically significant difference at P <0.05 between the three groups.
Figure 2 is a graphical representation of the anti-atopic efficacy of extracts in the atopic animal model compared to the concentration of IgE in serum:
Different alphabets of a, b, c indicate a statistically significant difference at P <0.05 between the three groups.

The present invention provides a pharmaceutical composition for the prevention and treatment of atopy containing chrysanthemum ( Chrysanthemum indicum Linne.) And a mixed extract of main rhubarb as an active ingredient.

The main rhubarb is preferably prepared by a manufacturing method comprising the following steps, but not always limited thereto:

1) mixing 10 to 15 roots of Hwangju (黄酒) with 50 to 120 roots of Rheum undulatum ;

2) after leaving the mixture of step 1) to steam; And

3) drying the steamed mixture of step 2).

In the above method, the rhubarb of step 1) is preferably from 50 to 120 roots, and from 10 to 15 roots of red wine, but not limited thereto. Preferred but not limited thereto.

In the above method, the mixture of step 3) is preferably steamed after being left for 4 hours, but not limited thereto, and is preferably steamed at 100 ° C. for 4 hours, but is not limited thereto.

In the above method, the steamed mixture of step 4) is preferably dried at 15 ° C to 30 ° C, but is not limited thereto, and is preferably dried at room temperature, but is not limited thereto.

The mixed extract is preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 40 parts by weight of main thickener, but is not limited thereto, and more preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 10 parts by weight of major sulfur, but is not limited thereto. .

The extract is extracted with a solvent selected from water, an alcohol or a mixture thereof, preferably a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof, 1,3-butylene glycol as an extraction solvent. It is preferable to use any one selected from the group consisting of, but is not limited thereto, and it is more preferable to extract using 1,3-butylene glycol, but is not limited thereto. The amount of the extraction solvent is preferably 10% to 40% by weight of the dry weight of chrysanthemum or main rhubarb, and more preferably 30% by weight, but is not limited thereto. The extraction temperature is preferably 10 ° C to 100 ° C, but is not limited thereto, and more preferably 40 ° C to 70 ° C, but is not limited thereto, and most preferably 60 ° C, but is not limited thereto. The extraction time is preferably 1 hour to 7 hours, more preferably 3 hours to 5 hours, but is not limited thereto, most preferably 2 hours, but is not limited thereto.

The method for preparing the extract is a method using an extraction device such as hot water extraction, dipping extraction, reflux cooling extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction method or adsorption resin including XAD and HP-20 Conventional extraction methods in the art, such as using a method may be used, preferably ultrasonic extraction at low temperature, but is not limited thereto.

In a specific embodiment of the present invention, the mixed extract of chrysanthemum and chimney rhubarb was prepared by adding 1,3-butylene glycol to chrysanthemum and main rhubarb, followed by low temperature ultrasonic extraction.

In addition, in a specific embodiment of the present invention, by treating the mite extract, which is an atopy-inducing substance in skin cells, by measuring the expression changes of PAR-2, ICAM-1 and IL-6 genes to determine the atopic induction concentration of the mite extract Confirmed at 3 ug / ml (see Table 1).

In addition, in a specific embodiment of the present invention, TNF-α and Il, which are inflammatory cytokines secreted into the medium after inducing atopic dermatitis, and treated with chrysanthemum, rhubarb, main rhubarb, and mixed extracts of chrysanthemum and main rhubarb By measuring the concentration of -6, compared with the individual extracts of chrysanthemum and major rhubarb, the mixed extract of chrysanthemum and major rhubarb significantly reduced cytokine secretion in atopic skin cells and showed a remarkable effect in alleviating the symptoms of atopic dermatitis. See Table 2).

In addition, in a specific embodiment of the present invention, in order to induce atopy on the skin cells, and treated with chrysanthemum, rhubarb, main rhubarb, and mixed extracts of chrysanthemum and main rhubarb, and then to confirm the change in activation of the NF-kB mechanism, When the NF-kB mechanism is activated, the expression of cytokines and adhesion molecules is increased, and as a result, the mixed extracts of chrysanthemum and main sulfur have atopic skin cells as compared to the individual extracts of chrysanthemum and major sulfur. Significantly reduced the expression of cytokines and adhesion molecules in, it was confirmed that the mixed extract of chrysanthemum and major rhubarb showed a significant effect in relieving the symptoms of atopic dermatitis by inhibiting the NF-kB mechanism (see Table 3).

In addition, in a specific embodiment of the present invention, NC / Nga mice were treated with DNCB (2,4-Dinitrochlorobenzene; Dinitrochlorobenzene) to prepare an atopic animal model inducing dermatitis symptoms. In NC / Nga mice, the form of atopic dermatitis accompanied by IgE overproduction is clinically and histologically very similar to that of human atopic dermatitis, and hypersensitivity reactions induced by X-ray irradiation and albumin It is very sensitive to the test animal, so it is easy to cause skin eczema wound such as atopic dermatitis in general environment. Butylene glycol as a control in the atopy animal model prepared as a control group by applying a mixed extract of chrysanthemum, main rhubarb, chrysanthemum and main rhubarb, the sensory evaluation using clinical visual evaluation results, chrysanthemum and major augmentation Compared with the individual extracts of sulfur, it was confirmed that the mixed extracts of chrysanthemum and major rhubarb showed a remarkable effect on improving the symptoms in the atopic animal model (see FIG. 1).

In addition, in a specific embodiment of the present invention, in order to confirm the anti-atopic effect of the mixed extract of chrysanthemum and major rhubarb through the change of immune function, the biggest problem of atopic dermatitis is that the inflammatory response is enhanced by abnormal activation of immune function. Since the incidence of cytokines is increased, the concentration of IgE in serum was confirmed to confirm abnormally enhanced immune function when atopic symptoms worsened. In the atopy animal model, butylene glycol was used as a control, and the experimental group was coated with a mixed extract of chrysanthemum, syrup rhubarb, chrysanthemum and syrup rhubarb. Then, blood was collected from capillaries of the ocular mucosa, and only serum was taken. As a result of confirming the concentration, the mixed extract of chrysanthemum and symptom rhubarb significantly reduced the concentration of IgE in atopic animal model and suppressed abnormal activation of immune function, compared with the individual extracts of chrysanthemum and symptom rhubarb. It was confirmed (see FIG. 2).

The composition comprising a mixed extract of chrysanthemum and syrup, according to the present invention, preferably comprises 0.1 to 50% by weight of the mixed extract of chrysanthemum and syrup, but not limited thereto.

The composition of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of a medicament.

The compositions according to the invention can be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. have. Examples of carriers, excipients and diluents that can be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient in the composition of the present invention, for example, starch, calcium carbonate, sucrose (sucrose), lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition of the present invention can be administered orally or parenterally, any parenteral administration method can be used, systemic or topical administration is possible, but systemic administration is more preferred, and intravenous administration is most preferred.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the preferred effect, the composition of the present invention is preferably administered at 0.0001 to 1 g / kg, preferably at 0.001 to 200 mg / kg. Administration may be administered once a day or may be divided several times. The dose is not intended to limit the scope of the invention in any way.

In addition, the present invention provides a dietary supplement for atopic prevention and improvement containing a mixed extract of chrysanthemum and main rhubarb as an active ingredient.

The mixed extract is preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 40 parts by weight of main thickener, but is not limited thereto, and more preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 10 parts by weight of major sulfur, but is not limited thereto. .

In a specific embodiment of the present invention, the mixed extract of chrysanthemum and major rhubarb significantly reduces the secretion of cytokines in skin cells induced by a mite, and reduces the expression of cytokines and adhesion molecules, as well as atopy animals. The model significantly improves clinical atopic symptoms and reduces the concentration of IgE in the blood, which is an immune function, and thus can be usefully used as a health food for the prevention and improvement of atopic dermatitis.

There is no particular limitation on the kind of food. Examples of foods to which the above-mentioned substances may be added include dairy products including drinks, meat, sausages, breads, biscuits, rice cakes, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, gums and ice cream, various soups, Beverages, alcoholic beverages, vitamin complexes, and the like, and include all healthy foods in the conventional sense.

The mixed extract of the chrysanthemum and syrup of the present invention may be added to the food as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its use purpose (for prevention or improvement). In general, the amount of the extract in the dietary supplement may be added to 0.1 to 90 parts by weight of the total food weight. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

The health functional beverage composition of the present invention has no particular limitation on the other ingredients other than the above-mentioned extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the chrysanthemum and the main rhubarb of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, such as flavoring agents, coloring and neutralizing agents (cheese, chocolate, etc.), pectic acid and Salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the mixed extract of chrysanthemum and jujube rhubarb of the present invention may contain pulp for the production of natural fruit juice and fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not so critical, but is usually selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the mixed extract of the chrysanthemum and main yellow rhubarb of the present invention.

In another aspect, the present invention provides a topical skin for preventing and improving atopy containing a mixed extract of chrysanthemum and main rhubarb as an active ingredient.

The mixed extract is preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 40 parts by weight of main thickener, but is not limited thereto, and more preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 10 parts by weight of major sulfur, but is not limited thereto. .

In a specific embodiment of the present invention, the mixed extract of chrysanthemum and major rhubarb significantly reduces the secretion of cytokines in skin cells induced by a mite, and reduces the expression of cytokines and adhesion molecules, as well as atopy animals. The model significantly improves clinical atopic symptoms and reduces the concentration of IgE in the blood, which is an immune function, and thus can be usefully used as an external skin preparation for atopic prevention and improvement.

The external skin preparation of the present invention is preferably made of a nanoliposomal formulation, but is not limited thereto.

The nanoliposomes are in the form of conventional liposomes, but the liposomes having an average particle diameter of 1-100 nm are preferred, but are not limited thereto, and more preferably 30-70 nm, but are not limited thereto. The liposomes are mutually spontaneous binding and aligned colloidal particles of particles composed of amphiphilic molecules with water-soluble heads and insoluble tails, and have a double-membrane structure similar to cell membranes. It can be expected to strengthen the skin and alleviate skin irritation.

_{Nanoliposomes} also include saturated or unsaturated fatty acids and intercellular lipids of C _12-22 alkyl chains. The saturated or unsaturated fatty acids of the C _12-22 alkyl chains used in the preparation of the nano liposomes of the present invention preferably include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid and palmilean acid More preferably, it may be any one selected from the group consisting of stearic acid, palmilean acid and mixtures thereof, but is not limited thereto, and the nano liposome may be contained in an amount of 0.05-5.0 wt% based on the total weight of the external skin preparation. However, the present invention is not limited thereto, and more preferably 0.1 to 4.0 wt%, but is not limited thereto. The content of the mixed extract of chrysanthemum and main rhubarb contained in the nanoliposomes is preferably 1.0-20.0 wt% based on the total weight of the nanoliposomes, but is not limited thereto, and more preferably 3.0-12.0 wt%, but is not limited thereto. It doesn't work.

When the mixed extract of chrysanthemum and syrup of the present invention is used as an external preparation for skin, it is further used as a fatty substance, an organic solvent, a dissolving agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizing agent and a foaming agent. , Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles Or any other ingredient commonly used in external preparations for the skin, such as auxiliaries commonly used in the field of dermatology. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.

In addition, the present invention provides a cosmetic composition for preventing and improving atopy, which contains a mixed extract of chrysanthemum and main rhubarb as an active ingredient.

The mixed extract is preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 40 parts by weight of main thickener, but is not limited thereto, and more preferably mixed in a ratio of 10 parts by weight of chrysanthemum and 10 parts by weight of major sulfur, but is not limited thereto. .

In a specific embodiment of the present invention, the mixed extract of chrysanthemum and major rhubarb significantly reduces the secretion of cytokines in skin cells induced by a mite, and reduces the expression of cytokines and adhesion molecules, as well as atopy animals. Significantly improves clinical atopic symptoms in the model, and reduces the concentration of IgE in the blood that exhibits immune function, and thus can be usefully used as a cosmetic composition for preventing and improving atopy.

The cosmetic composition of the present invention is preferably made of a nanoliposomal formulation, but is not limited thereto.

The nanoliposomes are in the form of conventional liposomes, but the liposomes having an average particle diameter of 1-100 nm are preferred, but are not limited thereto, and more preferably 30-70 nm, but are not limited thereto. The liposomes are mutually spontaneous binding and aligned colloidal particles of particles composed of amphiphilic molecules with water-soluble heads and insoluble tails, and have a double-membrane structure similar to cell membranes. It can be expected to strengthen the skin and alleviate skin irritation.

_{Nanoliposomes} also include saturated or unsaturated fatty acids and intercellular lipids of C _12-22 alkyl chains. The saturated or unsaturated fatty acids of the C _12-22 alkyl chains used in the preparation of the nano liposomes of the present invention preferably include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid and palmilean acid More preferably, it may be any one selected from the group consisting of stearic acid, palmilean acid and mixtures thereof, but is not limited thereto, and the nano liposome may be contained in an amount of 0.05-5.0 wt% based on the total weight of the external skin preparation. However, the present invention is not limited thereto, and more preferably 0.1 to 4.0 wt%, but is not limited thereto. The content of the mixed extract of chrysanthemum and main rhubarb contained in the nanoliposomes is preferably 1.0-20.0 wt% based on the total weight of the nanoliposomes, but is not limited thereto, and more preferably 3.0-12.0 wt%, but is not limited thereto. It doesn't work.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

In addition, the cosmetic composition is in addition to the mixed extract of the chrysanthemum and main rhubarb of the present invention, fatty substances, organic solvents, solubilizers, thickening and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents (foaming agents), Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or It may contain adjuvants conventionally used in the cosmetic field, such as any other ingredient conventionally used in cosmetics.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Preparation Examples.

However, the following Examples and Preparation Examples specifically illustrate the present invention, and the contents of the present invention are not limited to Examples, Experimental Examples, and Preparation Examples.

Example 1 Preparation of Extract

Chrysanthemum (Chrysanthemum indicum Linne.) And Rheum undulatum (purchased from Ulsan Gwangmyeongdang Pharmaceutical) are washed, and Juhwang rhubarb is mixed with 100 roots of rhubarb pieces and 14 roots of sulfur wine. And, after leaving for 4 hours and steamed for 4 hours at 100 ℃, prepare to dry in a well-ventilated place. Thereafter, 300 g of 1,3-butylene glycol (1,3-butylene glycol) is added to 1 kg of the prepared chrysanthemum, rhubarb, and main rhubarb, respectively, followed by low temperature ultrasonic extraction at 60 ° C. for 2 hours to obtain chrysanthemum, rhubarb, A mixed extract of chrysanthemum and main rhubarb was prepared.

Example 2 Skin Cell Culture

HaCaT cells (purchased from ATCC), human keratocytes, include 10% FBS, 2 mM L-glutamine, 100 IU / ml penicillin and 100 ug / ml streptomycin antibiotic Subcultured in RPMI medium.

Example 3 Preparation of Animal Model for Atopic Dermatitis

In order to confirm whether the mixed extract of chrysanthemum and symptom rhubarb is effective in atopic dermatitis, an animal model in which atopic dermatitis was induced was made. Specifically, 5-6 weeks old Nc / Nga mice (Japanese fancy mice, Nishiki-Nezumi, Japan) to clean the back area, left for 24 hours to heal the fine wounds of the epilation skin, 1 200 μl of% DNCB solution (acetone: olive oil = 3: 1) was applied to the back to cause dermatitis. After 4 days, 150ul of 0.2% DNCB solution is applied to the back area for 4 ~ 5 weeks every 2 ~ 3 times a week, so that dermatitis is sufficiently induced. As the scratching behavior was intensified, DNCB treatment was stopped, and atopic dermatitis animal models were prepared.

Experimental Example 1 Anti-Atopy Effect on Skin Cell HaCaT

<1-1> Changes in Expression of PAR-2, ICAM-1 and IL-6 by Mites Extracts

In order to confirm atopic dermal concentration by a mite treatment, which is an atopy-inducing substance in skin cells, skin mites were treated, and expression changes of PAR-2, ICAM-1 and IL6 genes were measured. Specifically, 0.3 ug / mL, 3 ug / mL, 30ug / ml of house mite extracts (purchased from Yonsei University Medical Arthropods) were collected on HaCaT cells, which are human keratocytes (keratinocytes) cultured in Example 2. And 300 ug / ml treatment and scraping the cells to homogenization buffer (homogenization buffer, 1 M HCl containing 5% (v / v) HCOOH, 1% (v / v) trifluoroacetic acid and 1% (w / v) NaCl) The amount of PAR-2, ICAM-1 and IL6 genes remaining in the cells extracted by alcohol extraction method was measured by real-time polymerase chain reaction (realtime PCR) method.

As a result, the expression of PAR-2, ICAM-1, and IL-6 genes was increased in proportion to the concentration of the mite extract, but it was not effective or the amount of expression was decreased at high concentrations above 3 ug / mL. Is shown (Table 1).

Therefore, when the mite extracts were treated on the skin cells, atopy was induced to increase the expression levels of PAR-2, ICAM-1, and IL6, and then, 3 ug / mL as a concentration for confirming the effect of the mixed extract. Was used a tick.

Changes in PAR-2, ICAM-1, and IL6 Expression by House Tick Extract in HaCaT Cells (Relative mRNA levels) PAR2 ICAM1 IL6 basal 1.21 ± 0.13 ^b 1.23 ± 0.14 ^b 1.23 ± 0.15 ^b 0.3 ug / mL mites 1.39 ± 0.18 ^ab 1.39 ± 0.17 ^b 1.49 ± 0.16 ^ab 3 ug / mL dust mites 1.50 ± 0.18 ^a 1.72 ± 0.21 ^a 1.63 ± 0.20 ^a 30 ug / mL dust mites 1.48 ± 0.17 ^a 1.67 ± 0.22 ^a 1.59 ± 0.18 ^a 300 ug / mL dust mites 1.42 ± 0.18 ^a 1.60 ± 0.24 ^a 1.55 ± 0.19 ^a

Different alphabets in columns a and b indicate statistically significant differences (p <0.05).

<1-2> Comparison of the concentrations of TNF-α and IL-6 secreted into the medium

To determine whether the mixed extracts of chrysanthemum, rhubarb, syrup rhubarb, chrysanthemum and syrup rhubarb affect cytokine expression in atopic dermatitis-derived skin cells, induced atopy on skin cells, treated each extract, and then secreted cytokines The concentration change of was measured. Specifically, 1 x 10 ⁶ HaCaT cells were transferred to 12 well culture plates 2 days before the cytokine secretion experiment, followed by RPMI with 10% FBS, 2 mM L-glutamine and antibiotics. Cultured in the medium. After incubation, 3ug / ml of the mite extract was treated on the day of the cytokine secretion experiment to induce atopy, and mixed 0.2% (w / v) BSA with chrysanthemum, rhubarb, main rhubarb, chrysanthemum and main rhubarb. The 30% extract of the extract was changed to KRB solution (pH 7.2) containing 2 ul / ml, and after 2 hours of treatment, KRB solution was collected from each well, and TNF-α, an inflammatory cytokines secreted into the collected KRB solution. And IL-6 concentrations were measured by ELISA kit (purchased from eBioscience).

As a result, the amount of TNF-α decreased in the order of the mixed extract of rhubarb, main rhubarb, chrysanthemum, chrysanthemum and main rhubarb compared to the control group, and IL-6 for rhubarb, chrysanthemum, main rhubarb, chrysanthemum and juju The amount of secretion decreased in the order of the mixed extract of augmented sulfur. Compared to the treatment of chrysanthemum and symptom rhubarb separately, when the mixed extract of chrysanthemum and symptom rhubarb was treated on atopic dermatitis-derived skin cells, it was confirmed that the amount of secretion of TNF-α and IL-6 decreased significantly. 2).

Therefore, compared to the individual extracts of chrysanthemum and symptom rhubarb, the mixed extract of chrysanthemum and symptom rhubarb significantly reduced cytokine secretion in atopic skin cells and showed a remarkable effect in alleviating symptoms on atopic dermatitis.

TNF-α (pg / mL) IL-6 (pg / mL) basal 456 ± 96 ^c 577 ± 121 ^c 3 ug / mL dust mites 1024 ± 184 ^a 1258 ± 214 ^a Mite + Chrysanthemum Extract 741 ± 126 ^b 907 ± 181 ^b Mite + Juju Rhubarb Extract 758 ± 159 ^b 870 ± 174 ^b Mite + Rhubarb Extract 887 ± 168 ^ab 1078 ± 192 ^ab Mite + Chrysanthemum + Juju Rhubarb Mixed Extract 605 ± 123 ^c 755 ± 167 ^c

a, b, c: Different alphabets in the same column indicate statistically significant difference (P <0.05).

<1-3> Confirmation of NF-kB Activation Change

When treated with a mixed extract of chrysanthemum and main rhubarb of the present invention in atopic dermatitis-derived skin cells, in order to confirm the change in activation of the NF-kB mechanism, cytokines (cytokine) is increased when the mechanism of NF-kB is activated And changes in expression of adhesion molecules. Specifically, 30% extract of a mite extract (3 ug / ml) and a mixed extract of chrysanthemum, rhubarb, main rhubarb, chrysanthemum and main rhubarb was treated together at 2 ul / ml for 2 hours in HaCaT cells, and then Cells were scraped and lysed with homogenization buffer, 1 M HCl containing 5% (v / v) HCOOH, 1% (v / v) trifluoroacetic acid and 1% (w / v) NaCl PAR-2, interleukin (IL) -6, intracellular adhesion molecules (IMC), and monocyte chemoattractant protein-1 (MCP) remaining in cells extracted by alcohol extraction method -1) The amount of expression was measured by real-time polymerase chain reaction (realtime PCR) method.

As a result, the expression of PAR-2, which is known to be increased when atopic symptoms appeared in skin cells, was increased in the treatment of mites extract, and PAR-2, ICAM-1, MCP-1, and Il-6 were rhubarb and symptomatic. Its expression decreased in the order of the extracts of rhubarb, chrysanthemum, chrysanthemum and main rhubarb, and the expression amounts of all cytokines and contacted molecules when the mixed extracts of chrysanthemum and main rhubarb were treated compared to the individual extracts of chrysanthemum and rhubarb. This was effectively reduced (Table 3).

Thus, compared to the individual extracts of chrysanthemum and syrup, the mixed extract of chrysanthemum and syrup is significantly reduced the expression of cytokines and adhesion molecules in atopic skin cells, whereby the extracts of chrysanthemum and symptom rhubarb have a mechanism of NF-kB. Inhibition was found to have a significant effect on alleviating the symptoms of atopic dermatitis.

PAR2 ICAM1 MCP-1 IL6 basal 1.19 ± 0.16 ^c 1.18 ± 0.18 ^c 1.21 ± 0.18 ^c 1.21 ± 0.19 ^c 3 ug / mL dust mites 1.66 ± 0.23 ^a 1.73 ± 0.24 ^a 1.86 ± 0.26 ^a 1.80 ± 0.23 ^a Mite + Chrysanthemum Extract 1.35 ± 0.19 ^b 1.31 ± 0.20 ^c 1.45 ± 0.19 ^b 1.34 ± 0.20 ^c Mite + Juju Rhubarb Extract 1.40 ± 0.18 ^b 1.37 ± 0.22 ^c 1.50 ± 0.21 ^b 1.40 ± 0.21 ^c Mite + Rhubarb Extract 1.57 ± 0.20 ^ab 1.52 ± 0.19 ^b 1.72 ± 0.22 ^ab 1.56 ± 0.21 ^b Mixed extract of mite + chrysanthemum and Juju Rhubarb 1.25 ± 0.16 ^c 1.12 ± 0.17 ^d 1.22 ± 0.18 ^c 1.18 ± 0.17 ^d

a, b, c, d: Different alphabets in the same column indicate statistically significant differences (P <0.05).

Experimental Example 2 Anti-Atopic Effects of Extracts in Atopic Animal Models

<2-1> sensory evaluation

In order to confirm the anti-atopic effect of the mixed extracts of chrysanthemum and major rhubarb in an atopic animal model, the extract was treated with an atopic dermatitis-induced animal model, and sensory evaluation was performed using clinical visual evaluation. Specifically, from the 10 days after the DNCB treatment in the animal model induced by atopic dermatitis by treating the DNCB prepared in <Example 3>, the control group was 30% 1,3-butylene glycol (1,3- butylene glycol), the experimental group was applied to the extract of chrysanthemum, main rhubarb and the mixed extract of chrysanthemum and main rhubarb twice a day for 8 weeks for 8 weeks, clinical visual evaluation method every two weeks Sensory evaluation was performed using. The visual evaluation results evaluated the five items, which are divided into erythema, pruritus & dry skin, edema and escoriation, erosion, and lichenification, respectively. The sum of one score is shown. Each of these items is graded as None (0), Weak (1), Severe (2), Severe (3), with scores ranging from a minimum of zero to a maximum of fifteen, and generally dermatitis using DNCB. If you trigger a 12-13 point can be judged that the dermatitis peaked.

As a result, there was a tendency to improve atopic status in all of the extracts of chrysanthemum, main rhubarb, chrysanthemum and main rhubarb compared to the control group treated with only butylene glycol alone. In particular, when treated with a mixed extract of chrysanthemum and symptom rhubarb compared to the individual extract of chrysanthemum and symptom rhubarb, atopic symptoms showed a marked improvement (Fig. 1).

Therefore, it was confirmed that the mixed extract of chrysanthemum and symptom rhubarb has a remarkable effect on improving the symptoms in an atopic animal model compared to the individual extracts of chrysanthemum and symptom rhubarb.

<2-2> blood IgE concentration

In order to confirm the anti-atopic effect of the mixed extracts of chrysanthemum and major rhubarb through changes in immune function, the extracts were treated in animal models in which atopic dermatitis was induced, and serum IgE concentration was measured by enzyme-linked immunoassay (ELISA). It was. Specifically, from the 10 days after the DNCB treatment in the animal model induced by atopic dermatitis by treating the DNCB prepared in <Example 3>, the control group was 30% butylene glycol (1,3-butylene glycol), The experimental group was applied to the stock solution of chrysanthemum, main rhubarb and chrysanthemum and main rhubarb mixed extract prepared in <Example 1> twice a day for 8 weeks, and then blood from the capillaries of the intraocular mucosa at intervals of 4 weeks. After collection and precipitation of blood cells, only serum was taken to check the concentration of IgE in the blood. ELISA dilutes capture antibody (anti-mouse IgE capture mAB, purchased from BD Bioscience) 250 times with coating buffer, add 50ul to 96well plate for ELISA, and overnight at 4 ° C. Incubated, allowed capture antibody to react on the plate, and then washed 5 times with PBST to remove unreacted antibody. Each well is then blocked with 100 ul of assay diluent at room temperature for 1 hour, and standard blocking (standard soln, 100/50/25 / 12.5 / 6.3 / 3.2) during blocking. /1.6 ng / ml) and 4.15ul of IgE standard samples, and sample samples were prepared. At this time, since the sample is a disease-induced clinical score is more than 6-7 points in the blood IgE concentration is usually 10ug / ml, it was used to dilute the blood 200-500 times. 50 ul of the standard sample and the sample sample were added to each well, covered with a wrap, and reacted at room temperature for 2 hours, and then washed 5 times with PBST. To each well 50 ul of detection Ab (Avidin-HRP) (Biotinylated anti-mouse IgE; purchased from BD Bioscience) was added to the wells and allowed to react at room temperature for 1 hour, followed by PBST. Rinse 5 times to remove unreacted antibody, add 50ul of TMB substrate to each well, and react for 30 minutes at room temperature. Then stop the reaction by adding 25ul of stop soln and absorbance at 450nm. By measuring, the concentration of IgE was measured.

As a result, the concentrations of IgE in the blood tended to decrease in both the group treated with the mixed extract of chrysanthemum, main rhubarb, chrysanthemum and main rhubarb compared to the control group treated with only butylene glycol alone. In particular, when the mixed extracts of chrysanthemum and symptom rhubarb were treated compared to the individual extracts of chrysanthemum and symptom rhubarb, the concentration of IgE in blood was significantly decreased (FIG. 2).

Therefore, the mixed extracts of chrysanthemum and symptom rhubarb significantly reduced the concentration of IgE in atopic animal models and suppressed abnormal activation of immune function compared to the individual extracts of chrysanthemum and symptom rhubarb and showed a marked improvement in atopic dermatitis. .

The preparation examples for the compositions of the present invention are illustrated below.

Preparation Example 1 Preparation of Pharmaceutical Formulation

<1-1> Preparation of powder

20 mg of mixed extract of chrysanthemum and syrup

Lactose 20 mg

After mixing the above components, the airtight cloth was filled to prepare a powder.

<1-2> Preparation of Tablet

10 mg of mixed extract of chrysanthemum and syrup

Corn starch 100 mg

100 mg of milk

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 1-3 > Preparation of capsules

10 mg of mixed extract of chrysanthemum and syrup

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2mg

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

<1-4> Preparation of Liquid

20 mg of mixed extract of chrysanthemum and syrup

10 g of isomerized sugar

5 g of mannitol

Purified water

According to the conventional method for preparing a liquid, each component is added and dissolved in purified water, lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 ml by adding purified water, and then filled in a brown bottle. The solution is prepared by sterilization.

<1-6> Preparation of Injection

10 ㎍ / ㎖ mixed extract of chrysanthemum and main rhubarb

Dilute hydrochloric acid BP until pH 7.6

Main sodium chloride BP up to 1 ml

Dissolve the mixed extract of chrysanthemum and main rhubarb in a suitable volume of main sodium chloride BP, adjust the pH of the resulting solution to pH 7.6 with dilute hydrochloric acid BP, adjust the volume with main volume of sodium chloride BP and mix well It was. The solution was filled into a 5 ml Type I ampoule made of clear glass, encapsulated under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120 ° C. for at least 15 minutes to prepare an injection solution.

Production Example 2 Preparation of Food

Food products containing a mixed extract of chrysanthemum and main rhubarb of the present invention were prepared as follows.

<2-1> Production of flour food

0.5 to 5.0 parts by weight of the mixed extract of chrysanthemum and syrup of the present invention was added to the flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare foods for health promotion.

<2-2> Production of Dairy Products

5 to 10 parts by weight of the mixed extract of chrysanthemum and main rhubarb of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<2-3> Preparation of Wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The mixed extract of chrysanthemum and main rhubarb of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a sprayer and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The dry powders of the grains, seeds, and mixed extracts of chrysanthemum and main rhubarb prepared above were formulated in the following ratio.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Mixed extract of chrysanthemum and syrup, (3 parts by weight),

(0.5 part by weight),

(0.5 parts by weight)

<2-4> Preparation of Health Supplements

100 mg of mixed extract of chrysanthemum and syrup

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the above-mentioned vitamin and mineral mixture is mixed with a composition suitable for a health food in a preferred embodiment, but the compounding ratio may be arbitrarily modified. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Preparation Example 3 Preparation of Health Beverage

       100 mg of mixed extract of chrysanthemum and syrup

       Citric acid 100 mg

       Oligosaccharide 100 mg

       Plum concentrate 2 mg

       Taurine 100 mg

       Add 500 ml of purified water

After mixing the above components according to the conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 1 L container, sealed sterilized and stored in the refrigerator and then Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and intended use.

Preparation Example 4 Preparation of Nanoliposomes

To prepare a nano liposome with the composition of the following [Table 4].

ingredient Content (% by weight) glycerin 15.0 Lecithin 5.0 cholesterol 5.0 Pseudoceramide 5.0 Stearic acid 2.0 Caprylic / capric triglyceride 5.0 Mixed Extract of Chrysanthemum and Juju Rhubarb 20.0 Purified water To 100

Production Example 5 Preparation of Cosmetics

<5-1> Softener (skin)

In order to prepare a flexible skin for atopic dermatitis comprising a mixed extract of chrysanthemum and main rhubarb of the present invention it can be formulated according to the preparation method in the field of conventional cosmetics as shown in the following [Table 5].

ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% 1,3-butylene glycol 3.0 glycerin 5.0 Polyoxyethylene (60) hardening castor oil 0.2 ethanol 8.0 Citric acid 0.02 Sodium citrate 0.06 antiseptic a very small amount Spices a very small amount Purified water To 100

<5-2> nutrition lotion (lotion)

In order to manufacture atopic dermatological nourishing water containing a mixed extract of chrysanthemum and symptom rhubarb of the present invention, it can be formulated according to the preparation method in the general cosmetic field as shown in the following [Table 6].

ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% 1,3-butylene glycol 8.0 glycerin 5.0 Squalane 10.0 Monooleic acid polyoxyethylene sorbitan 2.0 Flux oil 0.1 to 30% 1,3-butylene glycol 3.0 glycerin 5.0 Polyoxyethylene (60) hardening castor oil 0.2 ethanol 8.0 Citric acid 0.02 Sodium citrate 0.06 antiseptic a very small amount Spices a very small amount Purified water To 100

<5-3> essence

In order to prepare an atopic dermal essence comprising a mixed extract of chrysanthemum and main rhubarb of the present invention, it may be blended as described in the following [Table 7] and prepared according to a manufacturing method in a conventional cosmetic field.

ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceramide 0.7 Setares-4 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Carboxy Vinyl Polymer 0.2 Xanthan Gum 0.2 antiseptic a very small amount Spices a very small amount Purified water To 100

<5-4> Face wash (cleansing foam)

Atopy skin cleansing agent (cleansing foam) comprising a mixed extract of chrysanthemum and main rhubarb of the present invention can be prepared according to the conventional manufacturing method in the cosmetic field by blending as described in the following [Table 8].

ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% N-acyl glutamate 20.0 glycerin 10.0 PEG-400 15.0 Propylene glycol 10.0 POE (15) oleyl alcohol ether 3.0 Laurin derivative 2.0 Methyl paraben 0.2 EDTA-4Na 0.03 Spices 0.2 Purified water To 100

<5-5> Nutrition Cream

To prepare a nutritive skin cream for atopic dermatitis comprising a mixed extract of chrysanthemum and symptom rhubarb of the present invention, it is prepared according to the manufacturing method in the general cosmetic field as described in Table 9 below.

Compounding ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% vaseline 7.0 Liquid paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.5 Sorbitan sesquioleate 1.5 Squalane 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Xanthan Gum 0.5 Tocophenyl Acetate 0.1 Incense, preservative a very small amount Purified water To 100

<5-6> massage cream

To prepare a massage skin cream for atopic dermatitis comprising a mixed extract of chrysanthemum and symptom rhubarb of the present invention is prepared according to the manufacturing method in the general cosmetic field as described in Table 10 below.

Compounding ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocophenyl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Xanthan Gum 0.5 Incense, preservative a very small amount Purified water To 100

<5-7> pack

To prepare an atopic dermatological pack containing a mixed extract of chrysanthemum and syrup of the present invention, it is prepared according to the preparation method in the conventional cosmetic field as described in Table 11 below.

Compounding ingredient Content (wt%) Mixed Extract of Chrysanthemum and Juju Rhubarb 0.1 to 30% Propylene glycol 2.0 glycerin 4.0 Polyvinyl alcohol 10.0 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Incense, preservative a very small amount Purified water To 100

The present invention is not limited to the embodiments described above, and can be variously modified and changed by those skilled in the art, and can be applied to cosmetics of various uses, including other color cosmetics, It can be used in the manufacture of a medicament that can be applied thinly applied to the human body, that is, ointment, which is included in the spirit and scope of the present invention as defined in the appended claims.

As shown above, the mixed extract of chrysanthemum and main rhubarb of the present invention has little cytotoxicity and exhibits a marked improvement in atopic dermatitis. Thus, the development and products of external preparations, cosmetics, pharmaceuticals or functional foods for the treatment of atopy It can be usefully used for production.

Claims (9)

A pharmaceutical composition for preventing and treating atopic dermatitis comprising a mixed extract of chrysanthemum (Gamkook, Chrysanthemum indicum Linne.) And main rhubarb as an active ingredient.
The main symptom of claim 1 is
1) mixing 10 to 15 roots of Hwangju (黄酒) with 50 to 120 roots of Rheum undulatum ;
2) steaming after leaving the mixture of step 1); And
3) A pharmaceutical composition for preventing and treating atopy, characterized in that it is prepared by a method comprising the step of drying the steamed mixture of step 2).
The composition for preventing and treating atopy according to claim 1, wherein the extract is mixed at a ratio of 10 parts by weight of chrysanthemum and 10 parts by weight of major rhubarb.
The pharmaceutical composition of claim 1, wherein the extract is extracted with 1,3-butylene glycol (1,3-Butylene glycol).
Health functional food for the prevention and improvement of atopy containing mixed extract of chrysanthemum and Juju Rhubarb as an active ingredient.
A skin external preparation for preventing and improving atopic dermatitis, which contains a mixed extract of chrysanthemum and main rhubarb as an active ingredient.
According to claim 6, wherein the extract is a topical external preparation for atopy prevention and improvement, characterized in that the nanoliposome formulation.
Cosmetic composition for the prevention and improvement of atopic dermatitis containing a mixed extract of chrysanthemum and main rhubarb as an active ingredient.
The cosmetic composition for preventing and improving atopy according to claim 8, wherein the extract is a nanoliposome formulation.

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