KR20110118395A - Permeable barrier and method using the same for theremediation of contaminated ground water - Google Patents

Abstract

A permeable reaction wall and a groundwater purification method using the same are introduced to improve the safety of microorganisms and to effectively purify contaminants in groundwater. The groundwater purification method includes the steps of disposing a permeable reaction wall in which a specific microorganism is encapsulated and fixed in a carrier on a movement path of groundwater, supplying a substrate and oxygen to the permeable reaction wall, and in the permeable reaction wall. Inhaling the substrate on the opposite side of the permeable reaction wall supplied with the substrate for circulation, and decomposing the contaminants in the groundwater passing through the permeable reaction wall through biological reactions by specific microorganisms.

Description

Permeable reaction wall and method for purifying contaminated groundwater using the same {PERMEABLE BARRIER AND METHOD USING THE SAME FOR THEREMEDIATION OF CONTAMINATED GROUND WATER}

The present invention relates to a permeable reaction wall and a method for purifying contaminated groundwater using the same, and more particularly, a permeable reaction capable of purifying contaminants in groundwater by incorporating and fixing a specific microorganism capable of decomposing chlorine-based organic compounds in a carrier. It relates to a wall and a method for purifying polluted groundwater using the same.

The vast amount of waste generated by the acceleration of industrial development and population growth has seriously contaminated ground and groundwater. In particular, in the situation where the use of groundwater as industrial water, agricultural water and drinking water is increasing, interest in the groundwater quality is increasing, and various purification methods for improving the groundwater quality have been developed.

Purification methods to improve groundwater quality include physical methods such as excavation removal, surface control, shielding, pumping, vacuum extraction, solidification and hydraulic fracturing, and chemical methods such as oxidation, neutralization, ion exchange, and surfactant addition. , Thermal methods such as plasma, freezing, pyrolysis and vitrification, biological methods such as nutrient injection, use of specific microorganisms, and electrokinetic methods using electroosmosis.

Among these purification methods, a highly practical method may be a method of draining or adsorbing contaminants in groundwater by using a permeable reactive barrier. Permeable reaction walls for contaminated groundwater purification including a reaction medium containing microorganisms and contaminated groundwater purification method using the same (Registration No .: 10-0529300 (2005.11.10)) have been proposed.

However, in the case of the conventional technology, when microorganisms are directly supplied to the conventional permeable reaction wall, competition between the supplied microorganisms and the existing indigenous microorganisms is difficult. Be generated  In the end, due to the competition between these microorganisms there was a problem that the purification of contaminants in the groundwater using the microorganism was not effective.

An object of the present invention for solving this problem is to improve the safety of microorganisms for purifying the contaminants in the groundwater, permeable reaction wall that can improve the treatment capacity of the contaminants in the groundwater and groundwater purification method using the same To provide.

In order to achieve the above object, the contaminated groundwater purification method using the permeable reaction wall according to the present invention includes disposing a permeable reaction wall in which a specific microorganism is encapsulated and fixed in a carrier on the movement path of groundwater, and the permeable reaction. Supplying a substrate and oxygen to the wall, sucking the substrate on the opposite side of the permeable reaction wall to which the substrate is supplied so as to circulate inside the permeable reaction wall, and in the groundwater passing through the permeable reaction wall Degrading the contaminant through a biological reaction by a specific microorganism.

At this time, the step of decomposing, the specific microorganism in the carrier through the substrate supplied in the step of supplying the substrate and oxygen to release the degrading enzyme, the chlorine-based organic compounds in the contaminants through the degrading enzyme released from the specific microorganism It is preferable to decompose. The carrier is a three-dimensional network that forms crosslinks between backbones formed through polymer molecules, and is preferably composed of polyethylene glycol (PEG) -based polymer. The specific microorganism is preferably an aerobic microorganism including Pseudomonas cepacia G4 or Nitrosomonas europaea. It is preferable to supply toluene as a substrate to the Pseudomonas cepacia G4 microorganism and to supply nitrate as a substrate to the Nitrosomonas europaea microorganism.

The permeable reaction wall according to the present invention is a substrate having a specific microorganism encapsulated and fixed by a reaction wall network, and a substrate having a spray nozzle installed on one side of the case and supplying a substrate to the specific microorganism. And a substrate suction tube installed at the other side of the case and provided with a suction network for recovering the substrate supplied from the injection nozzle so as to circulate the substrate in the carrier.

Here, it is preferable that the plurality of substrate introduction tubes and the plurality of substrate suction tubes are arranged side by side to face each other with a plurality of carriers therebetween. The reaction wall network is preferably made of stainless steel or copper and has a body size smaller than the size of the carrier. The carrier is a three-dimensional network that forms crosslinks between backbones formed through polymer molecules, and is preferably composed of polyethylene glycol (PEG) -based polymer.

According to the present invention, the following remarkable effects can be realized.

First, in the present invention, unlike the physical and chemical methods of moving pollutants to other media for treatment, the process of treating pollutants is environmentally friendly by reducing the possibility of secondary pollution through biological methods utilizing specific microorganisms. Has the advantage.

Secondly, the present invention has an advantage that it is possible to easily decompose a hardly decomposable substance such as chlorine organic material which is difficult to decompose by a general biological treatment method through a common metabolism.

Third, the present invention prevents competition between the specific microorganisms of the present invention and indigenous microorganisms caused by growth substrates through the carrier, so that the specific microorganisms of the present invention that decompose contaminants in groundwater can continue to grow without being removed. The benefit is that it can be protected.

Fourth, the present invention has the advantage that by incorporating and fixing a specific microorganism in the carrier, it is possible to improve the treatment capacity of the contaminants, increase the useful life of the use of the permeable reaction wall, high efficiency biological purification and restoration.

Fifth, the present invention can prevent predation or loss from the outside by enclosing and fixing a specific microorganism in a carrier, and thus, there is an advantage that the efficiency can be improved compared to treatment under conventional anaerobic and aerobic single conditions.

1 is a conceptual diagram showing a permeable reaction wall and a contaminated groundwater purification method using the same according to the present invention.
Figure 2 is a block diagram showing a water-permeable reaction wall according to the present invention.
Figure 3 is a block diagram showing a substrate inlet tube and substrate suction tube of the permeable reaction wall according to the present invention.
Figure 4 is a block diagram showing a contaminated groundwater purification method using a permeable reaction wall according to the present invention.

First of all, in adding reference numerals to the components of each drawing, it should be noted that the same reference numerals are used as much as possible even if displayed on different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

Embodiments of the present invention will be described in detail with reference to the accompanying drawings.

As shown in Figures 1 to 3, the permeable reaction wall according to the present invention, the case 100, the substrate inlet pipe 200 and the substrate suction pipe 300, the configuration comprising a groundwater of contaminated ground ( 500 is installed on the moving path.

Specifically, the case 100 according to the present embodiment has a structure in which a carrier is accommodated by a reaction wall network (not shown). Here, the reaction wall network is made of stainless steel or copper material and has a body size smaller than the size of the carrier.

In particular, the reason why the carrier is used in the case 100 is to prevent the weakening of the competitiveness between the existing indigenous microorganisms through the comprehensive fixation of specific microorganisms. That is, various kinds of indigenous microorganisms exist in the groundwater 500 and the soil, and these indigenous microorganisms may use the same substrates provided to specific microorganisms of the present invention as growth substrates, and compete between these indigenous microorganisms and specific microorganisms. As relationships can be established, indigenous microorganisms can act as predators and certain microorganisms of the present invention can act as predators, and in order to prevent this, in this embodiment, by immobilizing a specific microorganism on a carrier, only a specific microorganism is protected. While being able to efficiently decompose contaminants in the groundwater (500).

In this case, the carrier is a three-dimensional network structure that forms crosslinks between backbones formed through polymer molecules, and is composed of polyethylene glycol (PEG) -based polymer. In particular, the fine pores formed in the carrier, the permeation of the substrate is possible, but the specific microorganisms in the interior or the external microorganisms can not be introduced into the inside, it can effectively protect the specific microorganisms from the outside.

Such carriers allow contaminants in groundwater 500, particularly Trichlethylene (TCE), to be biologically treated by certain microorganisms. TCE (Trichlroethylene) is a chlorinated aliphatic hydrocarbons (CAHs) used for dry cleaning of garments and for the removal of oil from mechanical and electrical components.Chlorine-based organic compounds (CHAs) are used throughout the world for soil and groundwater (500). Is the main source of pollution.

As specific microorganisms to be immobilized on the carrier, aerobic microorganisms including Pseudomonas cepacia G4 or Nitrosomonas europaea are used.

In this embodiment, the reason for using the aerobic microorganisms is that the decomposition reaction rate of TCE by the aerobic microorganisms is relatively fast, and carbon dioxide and chlorine ions converted into the final product are harmless to the human body. In particular, as studied, microorganisms that degrade TCE express oxygenase by a specific growth substrate, and by these enzymes, so-called cometabolism, (TCE) is removed, which can cleave harmful components using enzymes expressed by the substrate.

On the other hand, The use of anaerobic microorganisms in place of aerobic microorganisms, dichloroethylene (DCE: dichlorotehylene) in the anaerobic treatment process, the vinyl chloride (monochloroethene (VC: vinyl chloride) ) may be accumulated, vinyl chloride Ticino the ( TCE ) is much stronger than carcinogenicity. In addition, since the dechlorination reduction reaction rate and the growth rate of microorganisms is very slow, the process for removing TCE (TCE) is generated. In addition, considering that the DO concentration of the groundwater 500 is 2-3 mg / L, it is difficult to expect only anaerobic decomposition.

On the other hand, one side of the case 100 is provided with a substrate injection tube 200 for supplying a substrate to a specific microorganism in the carrier. Substrate injection pipe 200 to be introduced to the substrate which corresponds to the growth substrate for microorganisms within a specified carrier, the carrier wave of Pseudomonas three cyano G4 (a particular microorganism Pseudomonas cepacia G4) supplying a toluene (Toluene) as a substrate for micro-organisms and, in a particular microorganism carrier nitro consumption eggplant passage paeah (Nitrosomonas europaea ) supplies nitrates as substrates to microorganisms . Herein, the substrate refers to an electron donor that provides reducing power and energy necessary for growth or maintenance of cells, and the production of co-metabolizing enzymes and cofactors is induced by the substrate.

The substrate inlet tube 200 is composed of a plurality of spaced apart along the width direction on one side of the case 100, each substrate inlet tube 200 for supplying a substrate to a specific microorganism of the carrier in the corresponding longitudinal direction A plurality of injection nozzles 210 are provided, and a separate pump 400 is connected.

The other side of the case 100 is provided with a substrate suction tube 300 for recovering the substrate supplied from the substrate input tube 200. The substrate suction tube 300 allows the substrate to circulate in the carrier.

And the substrate suction tube 300 is composed of a plurality of spaced apart from the other side of the case 100 along the width direction, in this case, the plurality of substrate suction tube 300 is a plurality of substrate inlet tube 200 and the carrier therebetween. It is preferable to be arranged side by side to face each other. Each substrate suction pipe 300 is provided with a plurality of suction network 310 for recovering the substrate in the longitudinal direction, a separate pump 400 is connected.

Hereinafter, the present invention will be described in detail with reference to Examples.

Example 1

-Toluene, benzene, and phenol are supplied to Pseudomonas cepacia G4, a specific microorganism, as a first substrate. In addition, as a secondary substrate, the metabolic combibolism is performed in which the non-degradable contaminants are decomposed together, thereby removing the non-degradable contaminants such as TCE.

Primary substrate Reaction enzyme Secondary substrate Toluene Toluen monooxygenase Trichloroethylene Benzen Toluen monooxygenase Trichloroethylene phenol Toluen monooxygenase Trichloroethylene

[Example 2]

-When nitrate is supplied to a specific microorganism, Nitrosomonas europaea, as a primary substrate, and this microorganism decomposes the primary substrate and releases its degrading enzymes, it is a non-degradable contaminant as a secondary substrate. This breakdown cometabolism removes hardly degradable contaminants such as TCE.

Primary substrate Degrading enzyme Secondary substrate Nitrate AMO Trichloroethylene

On the other hand, the process of purifying the contaminants in the groundwater using the reaction wall described above is as follows.

As shown in FIG. 4, first, a carrier in which a specific microorganism is encapsulated and fixed is installed on a permeable reaction wall, and a permeable reaction wall equipped with a carrier is disposed on the groundwater 500 moving path of contaminated ground ( S1). At this time, the specific microorganism is an aerobic microorganism including Pseudomonas cepacia G4 (Nitrosomonas europaea) or Pseudomonas cepacia G4.

When the permeable reaction wall is disposed on the movement path of the groundwater 500, the substrate and the oxygen are supplied through the substrate inlet tube 200 provided at one side of the permeable reaction wall (S2). Preferably, toluene is supplied as a substrate when the specific microorganism in the carrier is Pseudomonas cepacia G4 microorganism, and when the specific microorganism in the carrier is a nitrosomonas europaea microorganism, Nitrate) is supplied as a substrate.

When the substrate and oxygen are supplied through the substrate inlet tube 200, the substrate is supplied through the substrate inlet tube 300 provided on the other side of the permeable reaction wall to circulate inside the permeable reaction wall (S3). ). Through this, the supply of the substrate is smoothly supplied to the specific microorganism of the carrier, the decomposition of the substrate can be made in the specific microorganism due to the supply of the substrate.

When the substrate and oxygen are smoothly supplied to the microorganisms, the biological reactions by the microorganisms are performed, and the decomposition of contaminants in the groundwater 500 passing through the permeable reaction wall is performed (S4). That is, when a substrate is supplied to a specific microorganism, the specific microorganism in the carrier releases the degrading enzyme, wherein contaminants in the groundwater 500 are formed by combibolism in which the hardly decomposable pollutants are degraded together as the secondary substrate. In particular, chlorine-based organic compounds such as TCE are decomposed, and eventually, the groundwater 500 contaminated with the contaminants is purified.

As described above, the present invention can easily decompose a hardly decomposable substance such as chlorine organic material which is difficult to decompose by a general biological treatment method through a common metabolism generated between a specific microorganism and a contaminant.

Although the present invention has been described in detail using the preferred embodiments, the scope of the present invention is not limited to the specific embodiments, and should be interpreted by the appended claims. In addition, those skilled in the art should understand that many modifications and variations are possible without departing from the scope of the present invention.

100: case 110: carrier
200: substrate injection pipe 210: spray nozzle
300: substrate suction pipe 310: suction network
400: pump 500: groundwater

Claims (9)

Disposing a permeable reaction wall in which a specific microorganism is encapsulated and fixed in a carrier on a movement path of groundwater;
Supplying a substrate and oxygen to the permeable reaction wall;
Inhaling the substrate on the opposite side of the permeable reactive wall to which the substrate is supplied so as to circulate inside the permeable reactive wall; And
And contaminating the contaminants in the groundwater passing through the permeable reaction wall through a biological reaction by a specific microorganism. The method according to claim 1,
In the decomposing step, the specific microorganism in the carrier releases the degrading enzyme through the substrate supplied in the step of supplying the substrate and the oxygen, and decomposes the chlorine-based organic compound among contaminants through the degrading enzyme released from the specific microorganism. Groundwater purification method using a permeable reaction wall, characterized in that. The method according to claim 1,
The carrier is a three-dimensional network structure that forms crosslinks between backbones formed through polymer molecules, and is made of a polymer of polyethylene glycol (PEG) based on a water-permeable reaction wall. Groundwater purification method. The method according to claim 2,
The specific microorganism is a contaminated groundwater purification method using a permeable reaction wall, characterized in that the aerobic microorganism comprising Pseudomonas cepacia G4 (Nitrosomonas europaea). The method of claim 4,
Permeable to the Pseudomonas cepacia G4 (Pseudomonas cepacia G4) microorganisms toluene (Toluene) as a substrate, the nitrosomonas europaea (Nitrosomonas europaea) microorganisms characterized in that the supply of nitrates (Nitrate) as a substrate Groundwater purification method using reaction wall. A case 100 in which a carrier having a specific microorganism encapsulated therein is accommodated by the reaction wall network;
A substrate injection tube (200) installed at one side of the case (100) and provided with a spray nozzle (210) for supplying a substrate to the specific microorganism; And
It is installed on the other side of the case 100 and characterized in that it comprises a substrate suction tube 300 is provided with a suction network 310 for recovering the substrate supplied from the injection nozzle 210 so that the substrate is circulated in the carrier Permeable reaction wall made of. The method of claim 6,
The case 100 has a plurality of substrate inlet tube 200 and a plurality of substrate inlet tube 300 is a water-permeable reaction wall, characterized in that arranged side by side to face each other with a plurality of carriers in between. The method of claim 6,
The reaction wall network is made of stainless steel or copper and permeable reaction wall, characterized in that having a body size smaller than the size of the carrier. The method of claim 6,
The carrier is a three-dimensional network structure that forms a crosslink between the backbone (backbone) formed through the polymer molecule, the water-permeable reaction wall, characterized in that composed of polyethylene glycol (PEG: polyethylene glycol) polymer.

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