KR20110115803A - Compositions for external application to the skin having a promotion activity of collagen synthesis - Google Patents

Abstract

The present invention discloses a composition for external skin preparation for promoting collagen production, which comprises an extract as a active ingredient.

Description

Composition for external application to promote collagen production {Compositions for External Application to the Skin Having a Promotion Activity of Collagen Synthesis}

The present invention relates to an external preparation composition for promoting collagen production. Specifically, the present invention is a ball dan ball hat ( Sargassum) Muticum ) relates to an external composition for skin promotion for collagen production using the extract.

Collagen is an insoluble crosslinked protein produced and secreted by the fibroblasts of the skin and is present in extracellular epilepsy and accounts for 30% of the total weight of biological proteins.

Collagen is a major fibrous protein that forms skin, bones, cartilage, blood vessels, etc.The main functions are collagen's mechanical strength, resistance of connective tissue and tissue binding, support of cell adhesion, induction of cell division and differentiation. It is known ( Ann NY Acad Sci . 1990; 580: 8-16).

In particular, collagen is composed of more than 90% of the dermal layer, when collagen is destroyed by ultraviolet rays, stress, free radicals, etc., the skin elasticity is reduced and skin wrinkles occur (Arch Dermatol. 1989; 125 (8): 1150). In addition, collagen has its own water retention, so when collagen is destroyed, the skin becomes dry and rough (J Ginseng Res 31 (2): 86-92, 2007;Toxicol Appl Pharmacol 195: 298-308, 2004).

Thus, collagen biosynthesis promoting material may be useful as a cosmetic raw material to prevent skin wrinkles and improve the dryness of the skin.

For this reason, efforts have been made to find collagen biosynthesis promoting substances. For example, Korean Patent Publication No. 2009-0110726 discloses an ultrafiltration permeate of aloe vera gel having collagen production promoting activity, and Korean Patent Laid-Open Publication No. 2006-0037245 discloses a milk-derived basic protein fraction or excitation having collagen production promoting activity. Discloses a basic peptide fraction treated with proteolytic enzymes.

The present invention also discloses a dumpling bead cap extract having collagen production promoting activity.

An object of the present invention is to provide an external composition for skin for promoting collagen production.

Other objects or specific aspects of the present invention will be presented below.

The present inventors confirmed that the biosynthesis of type I collagen is promoted when the hydrothermal extract of the transdermal bead capsula is treated with human fibroblast CCD-986SK, as confirmed in the following experimental example. Type I collagen is a collagen that has been reported to be closely associated with skin elasticity and skin moisturization ( J. Inv . Der. , 98; 248-254, 1992; Arch Dermatol 138: 1462-1470, 2002). In the following experimental example, it can be confirmed that the collagen biosynthesis promoting effect of the light bulb bead capillary extract is not due to the cell proliferation promoting effect.

The present invention was completed based on the results of these experiments, the composition for promoting external collagen production of the present invention is characterized in that it comprises a dumpling bead capillary extract as an active ingredient.

In the present specification, the meaning of "promoting collagen production" includes the meaning of "for improving skin wrinkles" and / or "for moisturizing skin," as described above, since the amount of collagen has a close correlation with skin wrinkles and skin moisturizing. I mean. Therefore, the skin external preparation composition for improving skin wrinkles comprising the dandelion bead capillary extract as an active ingredient and / or the skin external preparation composition for moisturizing the skin comprising the extract as an active ingredient is also included in the composition for promoting collagen production of the present invention. It is understood that.

In addition, in the present specification, "light bulb bead cap extract" regardless of the extraction method is used to extract the light bulb bead cap is methanol, distilled water, ethanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or a mixed solvent thereof The crude extract obtained by extraction with the crude extract and the crude extract is understood as meaning including the extract obtained by fractionation with the solvents listed above. Regardless of the extraction method, as long as the extraction object is extracted through the step of immersing in the extraction solvent, the extraction method may be any method such as cooling, refluxing, heating, ultrasonic radiation, and the like. Nevertheless, the "single bead hat jar extract" refers to an extract obtained by extracting the bead bead hat jar to be extracted with water, distilled water, ethanol or a mixed solvent thereof. Such extracts may be liquid concentrates or solids from which the extraction solvent has been removed.

In addition, the term "active ingredient" as used herein means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

Other terms that are not specifically defined herein follow the Korean dictionary meaning or the meaning generally accepted in the art.

On the other hand, the composition for promoting collagen biosynthesis of the present invention may include an effective ingredient, such as hardwood bead capsular extract, in any amount (effective amount) depending on the purpose, formulation, formulation purpose, etc., as long as it can exhibit collagen biosynthesis promoting activity. An effective amount will be determined within the range of 0.001% to 20.000% by weight, based on the total weight of the composition. Here, "effective amount" refers to the amount of the active ingredient that can promote the biosynthesis of collagen. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

The external preparation composition for promoting collagen biosynthesis of the present invention can be understood as a cosmetic composition in a specific embodiment.

When the composition of the present invention is conceived as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and Aqueous), anhydrous products (oil and glycol based), gels, masks, packs, powders and the like.

The composition of the present invention may include an acceptable carrier in a cosmetic preparation in addition to including the active ingredient.

Herein, "acceptable carrier in cosmetic preparation" means a compound or composition already known and used that can be included in cosmetic preparations or a compound or composition which will be developed in the future and does not have more toxicity than the body can adapt to when contacted with the skin.

The carrier may be included in the composition of the present invention from about 1% to about 99.99% by weight, preferably from about 50% to about 99% by weight of the composition.

However, it is understood that the ratios limit the scope of the present invention in any aspect because the ratios vary depending on the formulation of the cosmetics as described above and their specific application site (face or hand) or their preferred application amount. It should not be.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

The composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by including an active ingredient thereof in a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a hard water softener, and the like as an additive. .

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti or the like may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion sequestrant, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally added in an amount of 99% to 50% by weight based on the total weight of the soap composition. The additive may be added in an amount of 1% to 20% by weight.

The composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier, excipient, and the like in addition to the active ingredient, and may be a topical formulation such as creams, lotions, ointments (semi-solid external preparations), micro-emulsions, gels, pastes, transdermal preparations. (TTS) (such as patches, bandages, etc.) and the like.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) is not as toxic as applicable, without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, Solid lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, emulsifiers (such as TWEENS), wetting agents (Such as sodium lauryl sulfate), colorants, flavors, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions, and the like. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, suspending agents and dispersants such as sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethyl cellulose, sodium alginate, polyvinylpyrrolidone, etc. Can be.

The pharmaceutical composition of the present invention may be administered orally or parenterally, preferably topically.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

As described above, according to the present invention, it is possible to provide an external preparation composition for promoting collagen production. Collagen production promoting composition of the present invention can be used for skin wrinkle improvement or skin moisturizing use, it can be commercialized in cosmetics, soap and the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Dumpling ball hat  Extract manufacturer

Purified water of 10 times the weight of the sample was added to the dried sample of the hard bead cap, and hot water was extracted at 100 ° C. for 4 hours, and then the extraction residue was removed. The sample was dissolved in water and used.

< Experimental Example > Collagen biosynthesis promoting activity experiment and skin wrinkle improvement clinical trial

< Experimental Example  1> Collagen Biosynthesis Promoting Activity Experiment

<1> cell culture

Human fibroblasts (CCD-986SK, human fibroblasts) used in the experiment were purchased from the American Type Culture Collection (ATCC , Rockville, USA), 10% FBS (fetal bovine serum, Hyclone) and 1% Antibiotic-Antimycotic ( Cultured at 37 ° C., 5% CO ₂ conditions with DMEM (Dulbecco's Modified Eagle Medium.Hyclone) added with Gibco).

<2> Type I collagen synthesis promoting effect experiment

Human fibroblasts were dispensed into 96 wells at a concentration of 2 × 10 ⁴ cells / well and incubated for 24 hours. The medium was discarded and the sample was diluted with serum free medium and added to 13, 25, 50, 100, and 200 µg / ml concentrations and incubated for 24 hours. Cultures were collected and used for experiments, and collagen synthesis in cell cultures was measured using a procollagen type I peptide EIA kit (Takara Biomedical Co.). 100 μl of Anticody-POD conjugate solution was added to each well, and then 20 μl of sample and standard were added and incubated at 37 ° C. for 3 hours. After the reaction, the culture solution was removed from each well and washed four times with 400 μl of PBS (phosphate buffered saline). 100 μl of substrate solution was added and reacted at room temperature for 15 minutes. After stopping the reaction by adding 100 μl of stop solution (1N H ₂ SO ₄ ), the absorbance was measured at 450 nm with an ELISA reader. A standard concentration curve was prepared to check the amount of collagen.

The results are shown in FIG. 1, which shows that the treatment concentration significantly promotes biosynthesis of collagen at 50 μg / ml or more.

<3> cell viability evaluation experiment

Human fibroblasts are dispensed into 96 wells at a concentration of 2 × 10 ⁴ cells / well and incubated for 24 hours under cell culture conditions. The media was discarded and the samples were diluted with serum free media, treated with 13, 25, 50, 100, and 200 μg / ml concentrations and incubated for 24 hours. MTT solution (2mg / ml 3- (4,5-dimethylthiazol-2-yl) -2,5 diphenyl-2H-tetrazolium bromid solution) was added to each well of 50ul and incubated for 4 hours. After removing the culture solution, dimethyl sulfoxide (DMSO) solution was added, the solution was shaken for 10 minutes, and the absorbance was measured at 540 nm using an ELISA reader.

The results are shown in FIG. 1 along with the results of the type I collagen production promoting effect experiment.

From the results of Figure 1, it can be seen that the collagen biosynthesis promoting effect of the dandelion bead hatchban is not an effect of cell proliferation.

Statistical processing

One way ANOVA test was used to determine the effect, and Dunnett's post hoc test was compared to the control group for post test ( * P <0.05, ** P <0.01 ).

Claims (6)

Skin external composition for promoting collagen production, comprising a dumpling bead cap extract as an active ingredient.
The method of claim 1,
The extract is a skin external composition for promoting collagen production, characterized in that obtained by extracting the dumpling bead caps with water, ethanol or a mixed solvent thereof.
The method of claim 1,
The skin external preparation composition for promoting collagen production is a skin external preparation composition for promoting collagen production, characterized in that it is used for skin wrinkle improvement or skin moisturizing use.
4. The method according to any one of claims 1 to 3,
The composition for external application for skin, characterized in that the cosmetic composition.
4. The method according to any one of claims 1 to 3,
The composition for external application for skin, characterized in that the soap composition.
4. The method according to any one of claims 1 to 3,
The composition for external application of skin, characterized in that the pharmaceutical composition.

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