KR20110082819A - Agricultural nutrient using waste leaf of green tea - Google Patents

Abstract

PURPOSE: A plant nutrient using waste leaves of green tea is provided to increase plants and to ensure high storage property. CONSTITUTION: A plant nutrient contains waste leaves of green tea as an active ingredient, which is quickly pretreated under saturated steam of high temperature(200°C-250°C) and high pressure(20kgf/cm^2-40 kgf/cm^2). The plant nutrient additionally contains organic materials.

Description

Plant nutrient using waste leaf green tea {Agricultural nutrient using waste leaf of green tea}

The present invention relates to a phytonutrient using waste leaf green tea, which can increase the effect of enlargement and high shelf life of various plants, as well as increase the profits of green tea farmers by utilizing waste leaf green tea.

Recently, as interest in eco-friendly functional agricultural products has increased due to the interest in the public's health and well-being, the risk of toxicity in the use of persistent pesticides in agricultural products has become a concern of the public, and the use of pesticides for pathogens has been a concern. The resistance increased, the drug was lost and its use increased.

In this situation, consumers are increasingly interested in health and want to use the least harmful substances such as pesticide-free and organic. As a result, consumer-friendly food production has emerged as a major concern for agriculture. As a result, eco-friendly farming methods for restricting the use of chemical fertilizers and pesticides have been researched and developed at various angles, but the scope of application is very limited.

In particular, the development of plant growth nutrients from natural resources recognizes the potential of experts, but it is difficult to develop methods to extract effective extracts economically and inexpensively. Nevertheless, many studies are being conducted continuously.

First, plant-based biomass resources are organic resources with unlimited potential. It is a natural pollution-free resource, a resource produced by natural circulation and repetition in the category of the natural environment. Economically, however, transport and high added value are mostly degraded by the natural material cycle. However, plant-based biomass resources have the potential as phytonutrients, as they are a huge collection of carbon and have all the nutrients needed for plants.

Plant biomass resources are a large collection of carbon sources that can be classified as organic, most of which consist of carbohydrates, phenolic compounds, and other extracts such as organic acids and volatiles. Carbohydrates are present in the form of cellulose and hemicellulose. In addition to nitrogen, phosphoric acid, potassium, and trace elements, which are essential for plant growth, cellulose and hemicellulose are highly available as constituents involved in promoting plant growth. Especially, phenolic compounds Has the potential to improve the storage and disease resistance of plants, and organic acids and volatiles can be effective in controlling pests and plant pathogens.

Recently, several domestic companies have developed phytonutrients, but soil acidification due to the use of chemicals has been a problem for many years. Currently, phytonutrients in natural products have very weak technical level. In particular, the concept of plant growth nutrients produced in nature was also very inadequately recognized in Korea, but as the wind of environmentally friendly culture spreads to people recently, much attention has been focused on pesticide-free, low pesticides, and organic farming. In line with the current wind, many universities, agricultural technology centers, and fertilizer companies are participating in the development of natural plant nutrients. The materials are being developed using various materials such as timber, algae, and rocks. However, there are still difficulties due to various problems such as product cost and efficacy.

There have been no reports of plant nutritional supplements made from green tea, and the potential value of the plant nutritional supplement market in Korea is close to 1 trillion won.

In order to solve the problems of the prior art, the present invention adds a functional retention substance to the plant growth useful substances separated and extracted from the leaves and trees of Hadong green tea containing a large amount of various nutrients necessary for plant growth, promoting plant growth and physiology The primary purpose is to prepare the active agent, and the secondary purpose is to improve the profits of the local green tea farmers by utilizing the waste leaf green tea accordingly.

In order to achieve the above object, the present invention provides a phytonutrient using waste leaf green tea containing the waste leaf green tea pretreated for a short time under high temperature and high pressure saturated steam as an active ingredient.

The pretreatment according to the present invention is preferably pretreated with the waste leaf green tea for 3 to 9 minutes under a high temperature of 200 ℃ to 250 ℃ and saturated steam of 20 kg f / cm ² to 40 kg f / cm ² .

If out of the temperature range and the pressure range, the carbonization of the wood part according to the temperature and pressure may cause a relatively low concentration of carbohydrates and a change in volatile components due to wood deterioration.

In particular, if the pretreatment time is shorter than 3 minutes, the low molecular weight of the wood part is insufficient, and the content of the extracted component is lowered. If the pretreatment time is longer than 9 minutes, the deterioration degree of the wood part is severed, and the carbohydrate concentration of the extract is low and the volatile content is reduced. The content of is likely to increase relatively, which may cause problems that are unsuitable for use as a general purpose phytonutrient.

In addition, the phytonutrient according to the present invention preferably comprises 50 to 90 parts by weight of the pretreated waste leaf green tea extract based on 100 parts by weight of the phytonutrient. If it is included in a small amount out of the above range, it may not function as a phytonutrient, and if it is included in an excessive amount, it may not be economically desirable.

In addition, the phytonutrient according to the present invention may further include a mineral. In this case, the inorganic material may include potassium, iron, molybdenum, calcium, magnesium, phosphorus, zinc, iodine, and the like, but is not limited thereto, and may include any inorganic material that can be used as a phytonutrient.

The mineral is preferably included in 10 to 40 parts by weight based on 100 parts by weight of the plant nutrients. If the content of the mineral is out of the above range is low, there is a tendency to decrease the function as a plant nutrient, and if the content of the inorganic content exceeds 40 parts by weight, it is difficult to dissolve the mineral during the manufacturing process, manufacturing problems May cause.

In addition, the present invention comprises the steps of pre-treating waste leaf green tea for a short time under saturated steam of high temperature and high pressure; Instantaneously releasing and discharging the pretreated waste leaf green tea through a nozzle in the air; Extracting hot water by adding water to the seaweed waste green tea; And dehydrating and filtering the extract to provide a method for producing a plant nutrient using waste leaf green tea, characterized in that it comprises the step of separating the extract.

The pretreatment step is preferably pre-treated waste leaf green tea for 3 to 9 minutes under a high temperature of 200 ℃ to 250 ℃ and saturated steam of 20 kg f / cm ² to 40 kg f / cm ² .

In addition, the extraction step is preferably added to 5 to 9 parts by weight of water with respect to 1 part by weight of the seaweed waste leaf green tea to extract hot water for 30 to 60 minutes at 120 ℃.

In addition, the extract may further include a mineral.

The germination rate of lettuce seed according to the dilution of the plant nutrient according to the present invention was 250 times or the plant nutrient dilution 500 times dilution than the control was not germinated or similar to the control until 48 hours . When the plant nutrients diluted at 1000 times were compared with the control, the germination rate was higher than that of the control when the plant nutrient solution (HW) was applied to the lettuce seeds. Higher or similar.

The plant nutrients diluted 1000-fold showed good effect on germination rate, and the growth of red lettuce showed the good growth length of HW, while the green lettuce showed good growth length of NHW. Indicated.

The maximum growth length of each part of the plant was different, but the phytonutrients diluted 1000-fold for the lettuce species showed the best effect.

Therefore, the phytonutrient according to the present invention can be usefully used as a nutrient for a variety of plants, including large bonsai, strawberries, lettuce and the like.

Applying phytonutrients using waste leaf green tea according to the present invention to plants such as Daebonggam, strawberries and lettuce can not only increase the hypertrophy effect and high shelf life of these plants, but also increase the profit of green tea farmers by utilizing waste leaf green tea. You can.

Figure 1 shows the manufacturing process of the green tea nutrient according to the present invention,
Figure 2a and Figure 2b shows the crushed state of the waste leaf green tea before and after the waste leaf green tea pretreatment,
3 and 5 show the results of the red and lettuce seed germination test (elapsed three days) of the green tea nutrient solution (HW) according to Example 1, respectively,
4 and 6 show the germination rate of the red and blue lettuce seeds according to the germination period of the green tea nutrient solution (HW) according to Example 1, respectively,
7 and 9 show the results of the red and lettuce seed germination test (3 days) of the mineral-added green tea nutrient (NHW) according to Example 2, respectively,
8 and 10 show the germination rate of red and blue lettuce seeds according to the germination period of the mineral-added green tea nutrient (NHW) according to Example 2, respectively,
11 and 12 show the results of the growth (15 days) of red lettuce and blue lettuce by the green tea nutrient solution (HW),
13 and 14 show the results of growth (15 days) of red lettuce and blue lettuce by mineral-added green tea nutrient (NHW),
Figure 15 and Figure 16 shows the growth results for each part of red lettuce and blue lettuce by the green tea nutrient solution (HW),
FIG. 17 and FIG. 18 show the growth results of red lettuce and blue lettuce by mineral-added green tea nutrients (NHW), respectively.
19 shows the hypertrophy effect of the large peak feeling when the prototype containing inorganic green tea nutrient (NHW) and a functional product is applied to the large peak feeling,
FIG. 20 illustrates storage stability when a prototype including inorganic green tea nutrient (NHW) and a functional product is applied to a strawberry.
Figure 21 shows the change in hardness when applying a prototype containing a mineral-containing green tea nutrient (NHW) and functional products to strawberries,
FIG. 22 shows the storage stability when a prototype including inorganic green tea nutrient (NHW) and a functional product were applied to lettuce.

The present invention is explained in more detail by the following examples. However, the present invention is not limited by these examples.

Example 1 Preparation of Green Tea Nutritional Supplement

Waste leaf green tea raw material used in the present embodiment is a pruning operation using a cutter in the test green tea plantation located in Hadong Green Tea Research Institute, and since the leaves and branches are recovered at the same time to take into consideration the ease of operation, etc. Used.

This waste leaf green tea raw material is treated with a high temperature (over 200 ℃) and high pressure (20 ~ 40 kg f / cm ² ) saturated steam for a short time (3 ~ 9 minutes) as a pretreatment process, and then discharged instantaneously through the nozzle in the air. To optimize the extraction by frictional force, desalination was aimed, and to extract the extract, a certain amount of water was added (liquid ratio 9: 1), mixed, and then reacted at 120 ° C. for 30 to 60 minutes, followed by reaction residue, that is, high temperature. And the high-pressure treated sample and the reaction solution extract was separated by dehydration and filtration. The extract thus separated was used as a phytonutrient stock solution derived from waste green tea.

The crushed state of the waste leaf green tea by the high temperature and high pressure treatment as described above is shown in Figure 2b compared with the pre-treatment state of the waste leaf green tea (Fig. 2a).

Example 2 Preparation of Mineral-Added Plant Nutrients

To the 80.13 ml of the phytonutrient stock solution prepared in Example 1 was added in a ratio of 11.5 g of nitrate, 6.3 g of phosphate, 1.0 g of EDTA-FE, and 0.0043 g of sodium molybdate to prepare a mineral nutrient.

Example 3 Characterization of Plant Nutrients

1) pH measurement

Dilute 100 ml of the phytonutrient stock solution prepared in Example 1, add 2-3 drops of phenolphthalein indicator, and obtain the neutralization point of titration with 0.1 N NaOH solution, or 0.1 N NaOH until the pH reaches 8.15. Then, the consumption was measured to determine the acidity, and the pH was measured using a pH test paper or a pH meter.

The pH and specific gravity of the steam pretreatment extracts treated for 6 minutes at 25 atm from green tea leaves showed a pH range of 3.0-3.5 and specific gravity was 1.0046.

2) Analysis of Mineral Component Content

Inorganic component content contained in the plant nutrients prepared in Examples was ICP [inductively coupled plasma spectrometer; ICP-Atomic Emission Spectrometer, JY 38 Plus, Jobin-Yvon (France)]. At this time, the ICP analysis conditions were frequency: 40.66 MHz, grating: Daul 4320lines / mm + 1800lines / mm and mounting type: Czerny turner. The plasma gas flow rate, sheath gas flow rate, and sample flow rate were 12, 0.2, and 1 L / min, respectively.

As a result, as shown in Table 1, the contents of iron, boron, lead, and selenium were generally high, whereas zinc, aluminum, and magnesium were relatively low.

element Inorganic content, mg / g Example 1 Example 2 K 36.74 ± 4.73a 34.47 ± 3.66ab Ca 14.65 ± 2.60b 0.53 ± 0.13 g Mg  9.18 ± 1.05c 7.54 ± 0.76c Fe 0.21 ± 0.04i 1.27 ± 0.16f Mn 2.93 ± 0.50 g 0.07 ± 0.02i Zn 0.13 ± 0.03j - B 0.39 ± 0.43 h 6.63 ± 0.90d Al 4.05 ± 0.40f 0.10 ± 0.02h Na 6.94 ± 0.89d 5.37 ± 0.48e Pb 4.92 ± 0.80e 39.11 ± 5.84a Se 0.06 ± 0.04k 0.08 ± 0.03i ※ Below 0.05ppm is impossible to measure

3) Carbohydrate Analysis

The composition of carbohydrates contained in the plant nutrients prepared in Examples was analyzed by fructose calibration criteria by HPLC, and the content of carbohydrates was measured by DNS method. That is, 1 ml of the phytonutrient prepared in the example was added, and 3 ml of the prepared DNS reagent was added, and the mixture was left at 100 ° C. for 5 minutes and quenched with tap water. After quenching, the sample was measured for absorbance at 490 nm using an ultraviolet spectrophotometer, and the carbohydrate content was analyzed based on a previously prepared calibration curve.

Carbohydrate analysis includes Waters Co. 600E Model, Sugar-Pak I column (Φ6.5 × 300 mm) was used. The temperature of the column was maintained at 90 ℃, the mobile phase was used Ca-EDTA buffer (50mg Ca-EDTA / 1 LdH ₂ O). The flow rate was 0.5 ml / min, the injection volume was 10 μl, and the detector was analyzed using a Refractive Index Detetor (RI, Model 410). The results are shown in Table 2.

Form of sugar Sugar composition (%) Ara. Xyl. Man. Gal. Glu. Oligomer - 95.3 - - 4.7 Monomer 10.8 52.7 22.8 10.2 3.5

4) Volatile Components Analysis

In a vial bottle with a 50 ml silicone stopper, the nutrient sample prepared in the 5 ml example was accurately taken in the same amount as the control, and then 1 g of NaCl was added thereto. The temperature of the sample was maintained at 50 ± 2 ℃, the SPME was stabbed upright, and then volatile components in the sample was adsorbed onto SPME fiber (Supelco, 10㎛ polymethylsiloxane coating) for 30 minutes. The volatile components adsorbed on the SPME fiber were desorbed for 2 minutes at the injection hole set at 250 ° C., and then subjected to GC and GC-MS analysis.

For GC analysis, Shimadzu GC-17A, CBP 20 Capital column (0.22mm x 50m, film thickness 0.25㎛) was used. After the oven temperature was maintained at 100 ° C. for 2 minutes, the temperature was increased by 3 ° C. per minute to 220 ° C., and then maintained at 220 ° C. for 10 minutes. The inlet temperature is 250 ° C, the detector temperature is 230 ° C, and the flow rate of helium is 1.5 ml / min. The split ratio was 10. Shimadzu QP5050 was used for the GC-MS analysis and the same column as the GC analysis described above was used. Oven temperature, helium flow rate and split ratio were also measured under the same conditions as in GC analysis. The acceleration voltage was 70 eV and the interface temperature was 230 ° C. Most compounds were identified using MS library data. The results are shown in Table 3.

Peak No. RT (min) compound A One 20.13   Acetic acid 2.67 2 20.69   Furfural 0.90 3 22.95   Tetrahydrofurfuryl alcohol 0.88 4 24.35   2,3-Dimethyl cyclopenten-1-one 1.31 5 26.50   5-Methyl-2-furfural 0.83 6 28.77   Unknown 0.81 7 30.64   Unknown 0.70 8 39.68   3,4-Dimethoxy toluene 1.10 9 42.62   Guaiacol 5.87 10 43.19   3 (or 6) -Methyl guaiacol 1.03 11 47.04   6 (or 3) -Methyl guaiacol 0.81 12 47.60   cis-5-Butyldihydro-4-methyl-2 (3H) -furanone 1.82 13 47.85   4-Methyl guaiacol 9.45 14 48.17   Trimethyl phenols 0.71 15 48.38   3 (or 6) -Ethyl guaiacol 1.05 16 50.32   1-Indanone 1.48 17 50.60 o -Cresol 1.97 18 50.67   Phenol 1.69 19 50.98   - 0.73 20 51.97   Phenols 1.27 21 52.84   3-Ethyl syringol 1.87 22 53.05   Acetoguaiacone 0.66 23 53.43   Octanoic acid 1.16 24 54.17   2-Ethyl phenol 0.73 25 54.42   2,5 (or 4) -Xylenol 1.72 26 54.64   2,4 (or 5) -Xylenol 2.74 27 55.04 p- Cresol + m - Cresol 2.66 28 55.67   Acetosyringone 1.02 29 55.85   4-Propyl guaiacol 6.86 30 58.02   2,3-Xylenol 0.77 31 58.64   2-Phenyl-2-butenal 0.64 32 58.79   Eugenol 1.24 33 58.91   Ethylmethylphenols 1.41 34 59.09   Ethylmethylphenols 0.73 35 59.34   3,5-Xylenol 2.87 36 59.70   3-Ethyl phenol 0.78 37 61.01   Trimethyl phenols 0.83 38 61.58   3,4-Xylenol 1.29 39 61.74   Trimethyl phenols 1.10 40 63.16   trans-Isoeugenol 0.98 41 63.53   Ethylmethyl phenols 0.74 42 63.68   Syringol 6.88 43 67.74   4-Methyl syringol 8.12 44 70.43   4-Ethyl syringol 10.11 45 73.58   4-Propyl syringol 5.03

5) Organic and inorganic content analysis

2 g of the green tea nutrient sample prepared in Example was weighed into a crucible weighed in advance, and the stopper was slightly closed to heat and insulate, followed by complete carbonization in an electric furnace at 600 ± 25 ° C. The crucible was radiated on an asbestos plate for 2 minutes, and then cooled in a desiccator, followed by addition of a certain amount of 5% H ₂ O ₂ , followed by repeated ignition and cooling several times. The mineral content of mineral added phytonutrients added with minerals increased about 20% compared to the phytonutrient stock solution (Table 4).

The organic content was previously weighed with a weighing bottle and a thimble filter, and then 2 g of the green tea nutrient sample prepared in the above-mentioned example was transferred to a thimble filter, placed in a soxhlet, and placed in a flask of the extractor with 150-170 ml of 95% ethanol and benzene. (v: v = 1: 2) A mixture was added. The Soxhlet extractor was assembled and extracted for 6 hours in the heating mentle. After extraction, the thimble filter was taken out and the organic solvent was evaporated in the hood. The thimble filter containing the residue residue was transferred to a weighing bottle and dried for 24 hours at 105 ± 3 ℃ in a thermo-dryer, then cooled in a desiccator and weighed and measured. About 20% of the mineral supplemented plant nutrient was compared to the plant nutrient solution. Low results were shown (Table 4).

sample Mineral content,% Organic matter content,% Extraction stock solution a 14.1 14.6 ± 0.7 b a 85.9 85.4 ± 0.7 a b 13.9 b 86.1 c 15.1 c 84.9 d 13.7 d 86.3 e 14.9 e 85.1 f 15.7 f 84.3 Inorganic Ingredients a 35.0 34.1 ± 0.8 a a 65.0 65.9 ± 0.8 b b 34.7 b 65.3 c 34.0 c 66.0 d 33.9 d 66.1 e 34.5 e 65.5 f 32.7 f 67.3

6) Polyphenol Content Analysis

The polyphenol content was measured by adding 3 ml of distilled water to 30 μl of the green tea nutrient sample prepared in Example by modifying the Folin-denis method, and mixing 100 μl of Folin-Ciocalteu's phenolic reagent 2N reagent at room temperature for 5 minutes. After 5 minutes, 300 μl of a 20% Na ₂ CO ₃ solution was added, mixed well, and reacted at room temperature for 30 minutes, and the absorbance was measured at 765 nm with UV (U-3000 Spectrophotometer).

As a result, as shown in Table 5, the polyphenol content of the green tea nutrient preparation prepared in Example 1 was found to be 57.6 mg / g, and the polyphenol content of the mineral-added green tea nutrient prepared in Example 2 was 30.1 mg / g. appear. This suggests that the polyphenol content may decrease as the inorganic component is added.

sample Polyphenol content, mg / g Extraction stock solution a 57.1 57.6 ± 0.7 a b 58.2 c 56.4 d 58.0 e 58.1 f 57.5 Inorganic Ingredients a 30.0 30.1 ± 1.6 b b 28.9 c 28.0 d 32.9 e 30.7 f 29.8

Example 4 Plant Germination Rate Analysis of Plant Nutrients

Plant germination rate according to the application of the plant nutrient extract (HW) according to Example 1 and the mineral-added plant nutrient (NHW) according to Example 2 prepared above was evaluated.

1) Green Tea Nutritional Solution (HW) according to Example 1

i) Germination Rate of Green Tea Nutritional Supplements against Red Lettuce

The plant nutrient solution (HW) according to Example 1 was mixed with distilled water 250 times, 500 times, and 1000 times (based on the cooperative institution's recommended concentration), and the test product was treated with red lettuce seeds, and the control was treated with distilled water. . The germination rate of seeds was examined at 24 hours after tooth placement. One filter paper (Whaman No. 2) was placed in a 9 cm diameter Petri dish and 10 seeds were scattered thereon. The average value of 5 repetitions is shown. The epidermis of seeds and the root growth of more than 1mm were considered as germinated seeds, and the number of germinated seeds was examined at intervals of 24 hours, and the relative germinnation ratio (RGR) was calculated using the following equation.

[Equation 1]

The green tea nutrient solution (HW) according to Example 1 was treated by diluting 250-fold 500-fold and 1000-fold dilutions in red lettuce, and when diluted 1000-fold, the germination rate of HW was high (see FIGS. 3 and 4). And, as shown in Figure 3, even at a high concentration of 250 times dilution control and seed germination is not a big difference, which means that there is no harm (seed inhibition) of the seed by the green tea nutrient solution (HW).

In addition, as shown in Fig. 4, the germination rate of the control lettuce after 24 hours, the germination rate of the control was the highest, but after 48 hours, the plant-treated red lettuce showed the highest germination rate of 99.9%. That is, after 48 hours of diluting the plant nutrient to 1000ppm, the red lettuce seeds germinated 99.9%, while the control showed about 80% germination rate.

ii) Germination Rate of Green Tea Nutritional Supplements against Blue Lettuce

As a result of evaluating the germination rate according to the concentration of the phytonutrient stock solution (HW) according to Example 1 for the blue lettuce, the germination rate of HW was high when diluted 250 times in blue lettuce, as shown in Figure 5 Germination status of blue lettuce seeds showed similar germination with the naked eye as the germination status of red lettuce seeds.

In addition, as shown in Fig. 6, the lettuce seeds showed a lower tendency of germination compared to the control (water) in the early stages of germination, but showed a germination rate reaching 99.9% after 48 hours.

From these results, all seeds germinate over time, but it seems that the germination time can be reduced by treating plant nutrients, and green tea nutrients (HW) are weak at diluting levels of 250 times or more. Was judged to be little.

2) Inorganic-added green tea nutrient according to Example 2 (NHW)

i) Germination Rate of Mineral-Added Green Tea Nutritional Supplement (NHW) against Red Lettuce

As a result of evaluating the germination rate according to the concentration of the mineral-added green tea nutrient (NHW) according to Example 2 for red lettuce in the same manner, as shown in Fig. 7 showed a seed germination almost the same level as the control. In addition, as shown in FIG. 8, the germination rate of red lettuce for the mineral-added green tea nutrient (NHW) showed the highest result at 1000-fold dilution, and similar germination rate was shown after 72 hours at the remaining dilution concentration.

From these results, it could be inferred that even if the mineral-added green tea nutrient (NHW) was diluted to 250 times or more and applied to crops, the germination of seeds had no effect.

ii) Germination Rate by Mineral Concentration of Green Tea Nutrients (NHW)

As a result of evaluating the germination rate by concentration of the mineral-added green tea nutrient (NHW) according to Example 2 for blue lettuce in the same way, there was no difference between the degree of germination and distilled water at the naked eye as shown in FIG. In addition, the germination rate of the blue lettuce seeds over time as shown in Fig. 10, the germination rate of the distilled water as a control, the germination rate was higher than the dilution of NHW, as shown in Figure 10, except for 500 times dilution after 48 hours is almost the same level Showed germination rate of.

Therefore, the diluted solution of the mineral-added green tea nutrient (NHW) did not inhibit the germination of blue lettuce seeds, so it was judged that no harmful effects occurred when diluted more than 250 times with existing crops.

Example 5 Plant Growth Analysis According to the Concentration of Plant Nutrients

Plant growth was measured by applying the plant nutrient solution (HW) according to Example 1 prepared above and the mineral-added plant nutrient (NHW) according to Example 2 for each concentration.

That is, the green tea nutrient and the distilled water according to the embodiment are mixed at a predetermined ratio (based on the recommended concentration of the cooperative institution), and then the red lettuce and blue lettuce seeds are mixed with horticultural clay (supermix, fine clay). The mixture was mixed with water to adjust the specific gravity to 0.72 g / mL and the water content of 70%, and the red and blue lettuce seeds were sown, and the growth rate was investigated by fertilizing 10 mL daily.

1) Green Tea Nutritional Solution (HW) according to Example 1

Mix green tea nutrient solution (HW) and distilled water at a certain ratio (based on the cooperative institution's recommended concentration), and then mix red lettuce seeds with horticultural soil (supermix, fine soil) in a horticultural pot (Φ 6cm). The seedlings were seeded with 0.72 g / mL and 70% water content, and then seeded with red lettuce seeds and fermented at 10 mL daily for 15 days. As a result, as shown in FIG. 11, the red lettuce was visually higher than the distilled water as a control, and the growth was determined to be slightly higher at 250-fold and 1000-fold dilutions. In each concentration, when the extract was diluted 500 times than 250 times, it showed a low increase, and it was judged that the highest increase was obtained when the 1000 times diluted extract was treated.

In addition, the mixture of green tea nutrient solution (HW) and distilled water in a certain ratio was tested on the blue lettuce as described above, and as a result it shows a high increase when the nutrients are supplied compared to the control as shown in Figure 12, 1000 times It is believed that the highest increase is seen when the diluted extract is treated.

2) Inorganic-added green tea nutrient according to Example 2 (NHW)

Mix mineral-added green tea nutrient (NHW) with distilled water at a certain ratio (based on the cooperative institution's recommended concentration), then mix red lettuce seeds with horticultural soil (supermix, fine soil) with water in a horticultural pot (Φ 6 cm). The seeds were fertilized at 0.72 g / mL and 70% water content, and then the red lettuce seeds were sown and fertilized at 10 mL daily to investigate the growth of lettuce for 15 days. As a result, as shown in Figure 13, the red lettuce showed visually higher growth than the distilled water as a control, the length growth was confirmed that the highest 1000-fold dilution. In each concentration, when the extract was diluted 500 times than 250 times, it showed a low increase, and it was judged that the highest increase was obtained when the 1000 times diluted extract was treated.

In addition, mineral-added green tea nutrient (NHW) and distilled water were mixed in a proportion to experiment on the blue lettuce as described above. As a result, as shown in FIG. 14, there was no significant difference in the growth amount from the control water.

Example 6 Plant Growth Analysis According to Plant Part Concentrations of Plant Nutrients

The plant growth was evaluated by applying the plant nutrient solution (HW) according to Example 1 prepared above and the mineral-added plant nutrient (NHW) according to Example 2 according to the concentration of each plant in the same manner as in Example 5. .

1) Green Tea Nutritional Solution (HW) according to Example 1

i) red lettuce growth

That is, the green tea nutrient solution (HW) was diluted 250 times, 500 times, 1,000 times using distilled water, and the same control was applied using only distilled water. As a result, the green tea nutrient solution (HW) was used to grow red leaf lettuce. The growth effect was observed, and it was significant at the 5% level, the effect was also observed at the stem and root growth, and the significance was recognized at the 5% level.

Green tea nutrient solution (HW) had a higher influence on stem and root growth than leaves as shown in FIG. 15, and there was no constant trend according to the concentration. Therefore, the difference in growth according to the dilution concentration (250 times, 500 times, 1000 times) in leaf growth did not appear significant.

However, the 250-fold dilution showed the highest growth in stem growth, and significance was recognized at the 5% level. In root growth, the 1000-fold dilution showed higher growth than other concentrations, and the overall growth result was higher than that of distilled water.

ii) blue lettuce growth

As a result of applying the green tea nutrient solution (HW) to the growth of blue lettuce, the effect on the leaf growth as shown in Figure 16 did not appear significant, but in the stem and root growth was significant at 5% level was recognized. In particular, stem growth showed a distinct difference compared to the control, and the effect was better than the root growth.

In leaf growth, there was no significant difference according to the dilution concentration of the green tea nutrient solution (HW), and in the stem growth, there was no tendency of growth length according to the dilution concentration. In root growth, growth was not significant at 250-fold dilution, but at 500-fold dilution.

2) Inorganic-added green tea nutrient according to Example 2 (NHW)

i) red lettuce growth

As a result of applying mineral-added green tea nutrient (NHW, liquid type) to the growth of red lettuce, the effect on leaf growth did not appear as high as shown in FIG. 17, but the growth effect on the stem appeared to be significant at the 5% level. Compared to the distilled water, the control, the effect was clearly visible to the naked eye. Growth effect on root growth was significant when compared to the control at some concentrations, but the effect was insignificant.

Growth difference except for the stem part of red lettuce according to the dilution concentration of mineral supplemented green tea nutrient (NHW) (250 times, 500 times, 1000 times) was not significant compared to the control water, and finally the prototype for red lettuce growth The optimal dilution ratio of was determined to be 250-500 times.

ii) blue lettuce growth

As a result of applying the mineral-added green tea nutrient (NHW, liquid type) to the growth of blue lettuce, the growth effect on the leaves as shown in Figure 18 was significant, but the effect was low. However, in stem growth, NHW was significant at 5% level compared to the control, and the effect was clearly confirmed. Root growth was only significant at dilution concentrations greater than 250-fold compared to the control.

In stem growth, as the dilution concentration increased, the growth length tended to increase, and the stem growth length according to the dilution concentration was significant at 5% level.

As a result, based on the growth of blue lettuce, the optimal dilution concentration of mineral-added green tea nutrient (NHW) was determined to be 250 or 500 times.

Example 7 Farm Field Application Test of Mineral-Added Plant Nutrients (NHW)

1) Great feeling application test

The size and number of fruits is the most important factor in determining the success of persimmon farming. In order to confirm the hypertrophy effect by applying mineral-added plant nutrients (NHW), the persimmon orchard was divided into four sections, one section was used as a control, and the other three sections were sprayed three leaf and soil six times during the growing season. 6). In other words, Group A was prepared by adjusting the ratio of NHW and functional materials to 2: 8, Group B was prepared by adjusting the ratio of NHW and functional materials to 5: 5, and Group C was prepared by adjusting the ratio of NHW and functional materials. It was adjusted to 8: 2. The produced prototype was applied six times to the A, B, and C groups (50 pyeong) in Daebonggam Orchard.

Representative trees were selected, persimmons were harvested from the representative trees, and the number and weight were measured by a selector held by Hadong-gun Daebonggam Co., Ltd. Persimmons weighing 350 g or more are considered as commodities, and persimmons between 350 and 270 g are regarded as intermediate, and persimmons weighing 270 g or less are yawned. The lower the product value of the yawn, the lower the production rate of the yawn, the better the result.

In particular, the present experiment was focused on the weight of the harvested Daebonggam rather than the yield of Daebonggam. As a result, FIG. 19 shows the weight of the selected persimmon divided into upper, middle and lower products, and the number is expressed as a percentage. It can be seen that the proportion of goods increased. In the Group A, the upper commodities accounted for 50.18%, and in the Group B, 50.48%. Group C had a slightly lower upper ratio of 41.54%, but showed a 13% higher hypertrophy efficiency than the control with 28.57% upper ratio.

When to apply Application Fertilization method After flowering
(About June 12)  Hypertrophy 1ℓ Foliar fertilization
Soil spraying First phase
(About June 23) Calcium 1ℓ Foliar fertilization
Soil spraying First phase
(About July 10)  Hypertrophy 1ℓ Foliar fertilization
Soil spraying Second phase
(About July 25) Calcium 1ℓ Foliar fertilization
Soil spraying Second phase
(About August 15) Hypertrophy 1ℓ Foliar fertilization
Soil spraying Third phase
(About August 25) 1 l of colorant Foliar fertilization
Soil spraying

2) Strawberry coverage test

 There may be a number of tests to check the efficiency of nutrition by fertilizing a mineral-containing plant nutrient (NHW) in strawberries, but in the present embodiment, the strawberry, when the plant nutrient is fertilized by testing the hardness, sugar, and size of the strawberry fruit It was confirmed how much increased the commerciality compared to the control.

The demonstration gun selected strawberries produced from the Okjong in Hadong, and the house of the demonstration gun was 300 pyeong per building, and 150 pyeong was used for the facility. Nutrient, hypertrophy, calcium, and rooting agent were selected as the applied prototype (Table 7), and the prototypes were prepared by adjusting the ratio of green tea extract and functional material to 5: 5, B and C, ie, B type. Group C was tested by adjusting the ratio of green tea extract and functional material to 8: 2 until the first flower band after the establishment.

When the prototype was applied eight times to strawberries, there was no significant difference visually. There was no difference in the hypertrophy of strawberry fruit and no big difference in terms of production.

When to apply Application Fertilization method September 29 Rooting agent Irrigation (1.4ℓ per 150 pyeong) October 6 Nutrients Leaf noodles (400 ml per 150 pyeong) October 13 Calcium Irrigation (1.4ℓ per 150 pyeong) Nutrients Leaf noodles (400 ml per 150 pyeong) October 20 Hypertrophy Irrigation (3ℓ per 150 pyeong) Hypertrophy Leaf noodles (400 ml per 150 pyeong) October 27 Hypertrophy Irrigation (3ℓ per 150 pyeong) Calcium Leaf noodle (200 ml per 150 pyeong) November 3 Rooting agent Irrigation (1.4ℓ per 150 pyeong) Nutrients Leaf noodles (400 ml per 150 pyeong) November 10 Hypertrophy Irrigation (3ℓ per 150 pyeong) Nutrients Leaf noodles (400 ml per 150 pyeong) November 17 Hypertrophy Irrigation (3ℓ per 150 pyeong) Calcium Leaf noodle (200 ml per 150 pyeong) November 23
Calcium Irrigation (1.4ℓ per 150 pyeong) Nutrients Leaf noodles (400 ml per 150 pyeong)

In addition, the control and test plots harvested on the same day and in the same period for testing the shelf life of the strawberries were tested at room temperature. As shown in FIGS. 20 and 21, it can be confirmed that mold is formed in the control and the C test berries before and after 5 days. After about seven days, all the strawberries were moldy, but the progress was confirmed by the visually slow progress of strawberries harvested from B test.

In addition, the hardness test of the strawberry showed a better effect than the control. For numerical verification, 10 repeated hardness measurements were made using a hardness tester held by Hadong Green Tea Research Institute. The results are shown in FIG. 22.

As shown in Figure 22, when treated with phytonutrients using green tea can be seen that the hardness increased to about 20% or more than the control, the hardness does not affect 100% storage, but storage efficiency is increased as the hardness increases to some extent It can be judged to increase.

3) lettuce application test

 There may be a number of tests to check the efficiency of the nutrient by fertilizing the mineral-added plant nutrients (NHW) to the lettuce, but in this embodiment was tested with an emphasis on the shelf life of lettuce. For the demonstration gun, we selected lettuce farms grown in proper quantity in Hadong Pavilion, and applied 40 pyeong of the demonstration gun as an application unit. Nutrient, calcium and rooting agents were selected as the applied prototype (Table 8), and the prototypes were prepared by adjusting the ratio of green tea extract and functional material to 5: 5 in groups B and C. The ratio of green tea extract and functional material was adjusted to 8: 2, and was tested as the test period from 10 days after the formulation to the product was applied. As a result, there was no significant change with the naked eye, and both control and B and C test groups showed similar growth.

When to apply Application method Fertilization method October 20 Rooting agent Leaf surface (80 ml per 40 pyeong) November 3 Nutrients Leaf surface (80 ml per 40 pyeong) November 17 Calcium Leaf surface (40 ml per 40 pyeong) December 1st Calcium Leaf surface (40 ml per 40 pyeong)

Looking at the lettuce storage test results, it was seen that the leaves of the lettuce wither after 48 hours as shown in FIG. However, it was confirmed that the test zone B still preserves its shape. After 72 hours, the wounds of the control and lettuce lettuces were severely wilted, but the test B was retained to some extent.

Claims (7)

A plant nutrient using waste leaf green tea containing waste leaf green tea pretreated for a short time under high temperature and high pressure saturated steam as an active ingredient. The method of claim 1, wherein the pretreatment is a plant nutrient using waste leaf green tea for pre-treating waste leaf green tea for 3 to 9 minutes under a high temperature of 200 ℃ to 250 ℃ and saturated steam of 20 kg f / cm ² to 40 kg f / cm ² . The plant nutrient according to claim 1 or 2, further comprising waste green tea containing minerals. Pretreatment of the waste leaf green tea for a short time under high temperature and high pressure saturated steam;
Instantaneously releasing and discharging the pretreated waste leaf green tea through a nozzle in the air;
Extracting hot water by adding water to the seaweed waste green tea; And
Dehydrating and filtering the extract to separate the extract
Method of producing a plant nutrient using waste leaf green tea, characterized in that comprises a. The method of claim 4, wherein the pre-treatment step is a plant using waste leaf green tea for pre-treating waste leaf green tea for 3 to 9 minutes under high temperature of 200 ℃ to 250 ℃ and saturated steam of 20 kg f / cm ² to 40 kg f / cm ² Method of preparing nutrients. The method of claim 4, wherein the extracting step is added to 5 to 9 parts by weight of water based on 1 part by weight of seaweed waste green tea green tea plant extracts using waste leaf green tea extracted for 30 to 60 minutes at 120 ℃. The method of claim 4, wherein the plant extract using a foliar green tea further comprising a mineral in the extract.

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