KR20110047585A - Anti-obesity composition comprising Cordyceps militaris extracts - Google Patents

Abstract

PURPOSE: A composition containing Cordyceps militaris extract for anti-obesity is provided to reduce body fat and triglyceride in blood and to suppress fat accumulation. CONSTITUTION: An anti-obesity composition contains Cordyceps militaris extract. The Cordyceps militaris extract is isolated using water, hydrophilic organic solvent such as ethanol or mixture thereof. A functional food for weight loss contains the anti-obesity composition. The Cordyceps militaris extract is prepared by mixing 100g of Cordyceps militaris and 500 ml of ethanol; a step of filtering the mixture at 65°C for 2 hours three times; a step of decompressing at 45°C and removing a solvent; and a step of freeze-drying the concentrate.

Description

Anti-obesity composition comprising Cordyceps militaris extracts

The present invention relates to an anti-obesity composition comprising the extract of Cordillaps militaris and its use.

The present invention is derived from a study conducted as part of the Gyeonggi-do Strategic Technology Development Project.

[Project number: A08081910, Title: Development of anti-diabetic functional material using mushrooms, mushroom mycelium and spores]

In Korea, as in western developed countries, obesity is on the rise due to the recent increase in living standards and westernized diet. In 1996, the WHO defined obesity as a disease, and the Organization for Economic Cooperation and Development (OECD) recently announced that the country's proportion of obese populations has doubled in the United States and nearly three times in the United Kingdom and Australia compared to 20 years ago. Obesity is not just a cosmetic problem, but is a serious health problem because it leads to adult diseases such as hypertension, hyperlipidemia, arteriosclerosis, heart disease, type 2 diabetes, and the like.

Overweight and obesity occur because the amount of calories consumed and not consumed is converted to fat and accumulated in various parts of the body, especially the subcutaneous tissue and the abdominal cavity. Fat accumulation like this causes various diseases. As the body weight increases with fat accumulation, the incidence of hyperlipidemia and hypercholesterolemia increases due to the increase of cholesterol and triglycerides in the blood, and cardiovascular diseases such as angina pectoris, myocardial infarction and allergic hardening occur. In addition, insulin secreted by the pancreas, which controls blood sugar, has insulin resistance due to fat, which causes dysfunction, leading to diabetes.

Although most of the therapeutic agents developed to improve obesity are effective in weight loss and maintenance, there are questions about the sustainability of the effects, and problems such as depression, insomnia or digestive problems. Therefore, there is still a need for an anti-obesity agent that treats the underlying cause of obesity and has a continuous effect of improving obesity and can be safely administered without fear of side effects and toxicity.

Therefore, the present inventors have conducted studies on natural substances that act safely in the body while having an excellent anti-obesity and alleviation effect, so that the extract of Cordyceps militaris lowers blood sugar, reduces body fat and blood triglycerides. The present invention was found to have an effect of inhibiting the accumulation of fat in the body by increasing blood HDL (high density lipoprotein) -cholesterol.

It is an object of the present invention to provide an anti-obesity composition comprising militaris cordyceps extract.

Still another object of the present invention is to provide a functional food comprising an anti-obesity composition comprising the extract Militaris cordyceps.

In order to achieve the above object, the present invention provides an anti-obesity composition comprising a extract of Millyris cordyceps (Cordyceps militaris).

According to the Food and Drug Administration's database of raw materials, Cordyceps sinensis inhabits insects during winter, absorbs nutrients, destroys the host, and grows out of insects in summer. In other words, the spores or mycelium of Cordyceps fungus invade insects or fungal nuclei to create endogenous fungal nuclei in the host, and then form fruiting bodies outside.

According to the plant taxonomy, Cordyceps belongs to Ascomycota, Clavicipitales, Clavicipitaceae, and three genera of Cordyceps, Podonectria, and Torubiella. Among them, genus Cordyceps is representative (Kobayasi Y., Trans Mycol. Soc. Japan, 23. pp329-362, 1982). Militaris cordyceps, belonging to the genus Cordyceps, is distributed in Korea, Japan, and the United States and is also called pupa mushroom. Occurs in insect bodies in the land of mixed forests from summer to autumn. Parasitic on the pupa of lepidoptera insects. Fruiting bodies occur at the head of the chrysalis body, club-shaped, sack is round columnar, slightly curved, orange, but the head is fusiform and vivid orange. The pizzeria is embedded in the epidermis of the head, and there are thread-shaped spores in the inner scapula.

Militaris Cordyceps sinensis is known to have various effects such as anti-inflammatory, antibacterial, anti-tumor, and immune-enhancing effects such as protection, anti-aging, disease suppression and alleviation, but obesity prevention or treatment through inhibition of fat accumulation No effect was previously known of the present invention. The present inventors have found that the extract Millitaris cordyceps sinensis inhibits fat accumulation by decreasing blood triglycerides and increasing blood HDL-cholesterol.

In one embodiment of the present invention, the Millitaris Cordyceps Sinensis extract may be extracted by water, a hydrophilic organic solvent such as ethanol, or a mixed solvent thereof.

In one embodiment of the present invention, the Millitaris Cordyceps Sinensis extract may be an ethanol extract.

In one embodiment of the present invention, the Millitaris Cordyceps Sinensis extract may be an extract obtained by extraction, a dilution or concentrate of the extract, a dried product obtained by drying the extract or a crude or purified product of the extract.

In one embodiment of the present invention, the millitaris Cordyceps sinensis extract is mixed with 500 ml of Millitaris Cordyceps Sinensis with 500 ml of 80% ethanol, and the mixture is extracted three times for 2 hours by attaching a reflux condenser at 65 ℃ and filtered through a filter paper It can be prepared by the step of, the combined filtrate obtained above and concentrated under reduced pressure at 45 ℃ to remove the solvent, and the step of lyophilizing the obtained concentrate and can be stored at -70 ℃ until use.

In one embodiment of the present invention, the Millitaris Cordyceps Sinensis extract may be included as 0.02 to 90% by weight of the total weight of the composition. If the millitaris cordyceps sinensis extract is less than 0.02% by weight of the total weight of the composition, the anti-obesity effect is not detectable, and if it exceeds 90% by weight, the increase in anti-obesity effect is not large compared to the increased amount.

The anti-obesity composition according to the present invention may be included as an active ingredient in a pharmaceutical composition for preventing or treating obesity.

Pharmaceutical compositions comprising an anti-obesity composition according to the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. Pharmaceutically acceptable carriers, excipients and diluents that may be included in the pharmaceutical composition according to one embodiment of the invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, Alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and Including but not limited to mineral oils.

The pharmaceutical composition according to the present invention can be formulated and used in various formulations such as tablets, capsules, granules, suspensions, syrups and the like according to conventional methods known in the art.

The present invention also provides a functional food for weight loss comprising a composition comprising a Militaris Cordyceps Sinensis extract as a component.

Functional food for weight loss comprising the anti-obesity composition according to the present invention may be in the form of fish, meat, beverages, chocolate, snacks, confectionery, ramen, other noodles, gums, ice creams, vitamin complexes, fermented foods and the like.

As used herein, "functional food" means a food manufactured and processed using raw materials or ingredients having a useful function to the human body, and "functional" means nutrients or physiological effects on the structure and function of the human body. Ingestion means the purpose of obtaining a beneficial effect for the same health use.

In one embodiment of the present invention, the functional food for weight loss comprising the anti-obesity composition further comprises conventional food additives, such as flavors, flavors, colorants, fillers, stabilizers, etc. that are acceptable in food production. It may include.

Hereinafter, the present invention will be described in more detail with reference to experimental examples. However, the following examples are only intended to illustrate the invention and are not intended to limit the scope of the invention.

Anti-obesity composition comprising millilis cordyceps sinensis extract of the present invention can reduce body fat and blood triglycerides, increase blood HDL-cholesterol to inhibit fat accumulation, thereby effectively reducing weight to prevent and treat obesity have. The composition of the present invention can also be used for the treatment or prevention of diabetes because it reduces blood sugar.

Example 1 Preparation of Millitaris Cordyceps Sinensis Extract

100 g of Millitaris Cordyceps sinensis were prepared, and the medicinal herb was mixed with 500 ml of 80% ethanol, attached to a reflux condenser at 65 ° C., extracted three times for 2 hours, and then filtered through Whatman No. 2 filter paper. The combined filtrates were concentrated under reduced pressure at 45 ° C. to remove the solvent, and the lyophilized dry extract was stored at −70 ° C. until use.

Example 2 Anti-obesity Effect by Militaris Cordyceps Sinensis Extract

In order to confirm the anti-obesity effect of the extract of Millitaris cordyceps sinensis extract prepared in Example 1, the weight gain, dietary intake, and body fat weight were measured using experimental animals, and plasma was separated from blood to determine blood cholesterol and blood triglyceride content. Was measured. The experimental animals used were C57BL / 6 male black mice, weighing 17-19 g, 5 weeks of age, purchased from Daehan Biolink.

2-1. Weight gain suppression

In order to confirm the inhibitory effect on the weight gain of the Militaris cordyceps sinensis extract prepared in Example 1, the weight gain and dietary intake of the experimental animals were measured for a certain period of time. Body weight gain and dietary intake were measured as follows. The experimental animals were divided into two groups, the normal diet and the high-fat diet, and each group consisted of 6 mice. The normal diet (# 38057, Cargill Agri Purina Inc., Republic of Korea) supplied 4.5% of total calories to fat, and the high-fat diet (# D12492, Research Diets Inc., United States of America) produced 60% of total calories. % Was fed as fat and raised. The dietary composition supplied to the normal diet and the high fat diet group is shown in Table 1 below.

Table 1. Dietary Composition

ingredient Normal diet. It's high-fat. Normal diet (n = 6) High fat diet (n = 6) High Fat Diet + Militaris Cordyceps Sinensis Extract (n = 6) protein 20 20 20 Fat 4.5 60 60 carbohydrate 64.1 20 19.95 Fibrous 6 mineral 4.2 vitamin 1.2 Militaris Cordyceps Sinensis Extract 0.05 Total amount (% by weight) 100 100 100

After adapting the experimental animals to laboratory conditions for one week, the diet (normal diet group), high fat diet (high diet control), and high fat diet and Militaris cordyceps extract 0.05 weight each for 4 weeks from 6 to 10 weeks of age. Dietary supplements containing% (high-fat diet group) were fed to observe the effect of inhibiting weight gain by Militaris cordyceps extract. 1 shows the weight change by dietary feeding of these conditions.

Experimental animals were bred in three groups for each group, and water and diet were supplied without restriction. Dietary intake and body weight were measured twice a week during the experiment. The food efficiency ratio (FER) was calculated by dividing the amount of weight gain during the test period by dividing the dietary intake during the test period from the experimental diet supply date to the sacrifice day. The results are shown in Table 2 as weight gain and dietary intake for 4 weeks for each experimental group.

Table 2. Effect of Militaris Cordyceps Sinensis Extract on Weight Gain

Total weight gain (A, g) Total dietary intake (B, g) Dietary Efficiency (A / B * 100)
Normal diet 4.00 93.95 4.26
High-fat diet 4.63 64.93 7.14 High Fat Diet + Militaris Cordyceps 0.05% by weight 4.33 66.60 6.51

* All data are displayed as mean ± standard deviation.

As shown in Table 2, in the high-fat diet group in which the diet which added 0.05% of millitaris cordyceps extract to the high-fat diet was added to the diet, the high-fat diet only ingesting the high-fat diet increased weight compared to the control group. Decreased by about 6%. In particular, the dietary intake was lower in the high-fat diet group and the high-fat diet group than the normal diet group during the whole period, but the weight gain was greater due to the high calorie intake of fat, so that the dietary efficiency (weight gain / diet intake * 100) was higher. Lee group was higher. However, the high-fat diet with 0.05 wt% of Militaris cordyceps sinensis extract in combination with the high-fat diet was found to be about 9% lower than the high-fat diet, even though the high-fat diet was consumed. .

2-2. Inhibit body fat accumulation

In order to confirm the inhibitory effect on the increase of body fat of the Militaris cordyceps sinensis extract prepared in Example 1, the body fat of the experimental animal was separated and its weight was measured.

As described in 2-1 above, the animals fed with normal diet, high fat diet and high fat diet + Militaris Cordyceps sinensis extract, respectively, were sacrificed by two or three animals in each experimental group at the time of 10 weeks of age. And organs were harvested. Visceral fat, kidney fat (peri-kidney fat) and epididymal fat were separated from the organs and their weights were measured. The results are shown in Table 3 below, the fat weight (fat weight per 100 g body weight) for each part of the experimental group.

Table 3. Effect of Militaris Cordyceps Sinensis Extract on Body Fat Weight

Visceral fat (g / 100g body weight) Kidney Fat (g / 100g Weight) Epididymal fat (g / 100g body weight) % Of total fat * Normal diet 0.97 0.44 1.49 56 High fat diet 1.26 1.09 2.83 100 High Fat Diet + Militaris Cordyceps 0.05% by weight 0.80 1.09 2.72 95

* Total fat is the sum of visceral fat, kidney fat and epididymal fat.

Table 3 shows that the difference in body weight gain with high fat diet is due to the difference in body fat accumulation. The percentage of total fat is the sum of the weights of the total fat (visceral fat, kidney fat, and epididymal fat) in each group relative to the high fat diet. In the high-fat diet group, which had high fat diet and Militaris cordyceps extract, the high-fat diet, which was fed only high-fat diet, reduced body fat weight by 5% compared to the control group, especially visceral fat group. Fat accumulation was reduced more.

2-3. Inhibition of blood cholesterol and triglyceride accumulation

In order to confirm the effect on the blood cholesterol and triglyceride content of the Militaris cordyceps sinensis extract prepared in Example 1, the amount of cholesterol and triglycerides in blood of the experimental animals was measured. Measurement of blood cholesterol and triglyceride content using experimental animals was performed as follows.

As described in Example 2-2, blood collected by orbital vein immediately before sacrifice of the experimental animal was centrifuged at 4000 rpm for 10 minutes to obtain plasma and stored at −70 ° C. until analysis. Plasma total cholesterol, triglycerides and HDL-cholesterol were measured by enzyme colorimetric method using a kit commercially available from Asan Pharmaceuticals (T-CHO, Cleantech TG-S, HDL-CHO, Asan Pharmaceutical, Korea).

Table 4. Effect of Militaris Cordyceps Sinensis Extract on Blood Cholesterol and Triglyceride Contents

Total cholesterol (mg / dl) HDL-cholesterol (mg / dl) Triglycerides (mg / dl) Normal diet 121.64 99.11 150.69 High fat diet 182.98 146.70 163.02 High Fat Diet + Militaris Cordyceps 0.05% by weight 210.37 162.76 144.67

As shown in Table 4, the total cholesterol content of blood was increased in the high-fat diet group, which was fed high-fat diet and Militaris cordyceps extract, but the high-fat diet was fed only high-fat diet, compared to the control group. Lowering useful HDL-cholesterol was observed in the high-fat diet group by about 11% more than the high-fat diet group. Serum triglycerides increased by about 8% in the high-fat diet control group compared to the normal diet group, while the high-fat diet group fed with Millitaris cordyceps extract was significantly reduced, which is about 89% higher than the normal diet group. Lower.

Example 3 Antidiabetic Effect by Militaris Cordyceps Sinensis Extract

In order to confirm the hypoglycemic effect of the Millitaris cordyceps sinensis extract prepared in Example 1, the blood glucose content was measured by separating plasma from blood using an experimental animal as described in Example 2. Plasma blood glucose stored at −70 ° C. after blood collection and separation from experimental animals was measured by enzyme colorimetry using a kit commercially available from Asan Pharmaceuticals (GLUCOSE, Asan Pharmaceuticals, Korea).

Table 5. Effect of Militaris Cordyceps Sinensis Extract on Blood Sugar

Blood sugar (mg / dl) Normal diet 158.41 High fat diet 181.25 High Fat Diet + Militaris Cordyceps 0.05% by weight 163.73

As shown in Table 5, in the high-fat diet experimental group ingested Militaris Cordyceps sinensis extract with the high-fat diet, high-fat diet only high-fat diet showed a decrease in blood glucose compared to the control group. The high-fat diet control group with increased blood sugar by the high-fat diet increased the blood sugar by about 29% compared to the normal diet group, while the high-fat diet group fed the high-fat diet with Militaris cordyceps extract added the high-fat diet control group. Blood sugar was reduced by about 10%.

Figure 1 shows the weight change over time of the normal diet group (ND), high-fat diet control group (HFD), and the high-fat diet group (HFD + CM (0.05%)).

Claims (3)

Anti-obesity composition comprising an extract of Millyris cordyceps ( Cordyceps militaris ). The anti-obesity composition of claim 1, wherein the millilis cordyceps sinensis extract is an ethanol extract. Functional food for weight loss comprising the composition of claim 1.

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