KR20100136710A - Natural antibiotics based on natural products and its composition and function - Google Patents

Abstract

PURPOSE: A composition for suppressing growth of in vivo harmful bacteria containing medicinal herb mixture extract is provided to control harmful bacteria without side effects and tolerance and to treat plant blight. CONSTITUTION: A composition for suppressing growth of in vivo harmful bacteria contains medicinal herb mixture extract containing extract of 0.1-6 weight parts of Copitis chinensis, 0.1-6 weight parts of Phellodendri cortex, or 0.1-4 weight parts of Schisandra chinensis as an active ingredient. The harmful bacteria are S. pyogenes, S. zooepidemicus, C. difficile, C. perfringens, S. aureus or S. epidermidis. The extract is isolated using water, alcohol or mixture thereof.

Description

Natural Antibiotics Based on Natural Products and Its Composition and Function

The present invention relates to a natural antibiotic comprising the herbal extract as an active ingredient.

Along with the improvement of living standards, the demand for edible meat is greatly increasing, and accordingly, the supply is also increasing. However, the quality of meat will be a problem as everyone pays attention to the health of their families in the age of well-being. In particular, the problem caused by the abuse of antibiotics is serious and these synthetic antibiotics are a major cause of increasing the resistance to bacteria entering the human body. In addition, marine fish and meat for export are not stable because of the abuse of antibiotics, causing serious damage to farmers and fishermen who are producers. In addition, in order to lower the production cost, the number of animals per unit area is too large, the stress for survival increases, and is always exposed to the pathogenic microorganisms by feces and the like and are exposed to the infection of various diseases. In order to prevent or treat such diseases, various chemotherapeutic agents are widely used. At this time, a drug is not properly selected, commercially available or long-term administration is exacerbating the disease without a therapeutic effect. In addition, the bacteria themselves have increased resistance to the drug, the concentration of the therapeutic agent must be used day by day, and several types of drugs are used in parallel, so in some cases, the newly used drugs do not show a clear therapeutic effect. to be.

Recently, research on the development of feed and feed additives and the development of immunostimulants to improve the self-defense ability of food animals against diseases. Much research and time will be needed to make them practical and universal enough to regulate drug use. Moreover, in recent years, distrust of consumers has increased due to the presence of antibiotics in meat tissues, and environmental hormones are emerging with sensitivity. And although the accumulation of antibiotics along the food chain is harmless to living organisms, the direct injection of antibiotics makes all bacteria resistant, and when these resistant bacteria are administered in vivo, there is no evidence of a recent superbacteria, such as superbacteria. Uncontrollable cells, even as powerful drugs, may develop and threaten human health. As such, the administration of an excessive amount of antibiotics for the treatment of edible meat diseases may threaten the stability of foods, which may lead to a decrease in commodities, which inevitably leads to large economic losses in livestock prices. Therefore, even if the used therapeutic agent remains in meat, it is necessary to develop an antibiotic that does not cause harm to the person who consumes it and can be quickly and easily decomposed when exposed to the ecosystem, and thus does not cause secondary problems caused by antibiotics. In addition, the mixing of antibiotics and antimicrobials in veterinary medicines is permitted until 2011, and will be banned from 2012, and the Ministry of Agriculture and Forestry will gradually reduce the mix of antibiotics and antimicrobials in formulated feeds to produce safe livestock products. Of the 25 antibiotics and antimicrobials currently available, seven such as tetracycline antibiotics and five antibiotics are not allowed to be added to blended feeds from 2009. Overseas, the World Health Organization (WHO) warns of the dangers of using antibiotics for livestock feed and regulates the EU from administering antibiotics outside of therapeutic purposes. The US Food and Drug Administration (FDA) has banned the use of new antibiotics in addition to therapeutic purposes. In 1999, farm livestock consumed 4,700 tons, 35% of all EU-approved antibiotics, but humans consumed 8,500 tons, 65%. Of the antibiotics consumed by cattle, 3,900 tonnes (29% of total use) were used to treat livestock disease, but 786 tonnes (6%) of antibiotics used as growth promoters in feed were reported.

At this point, the development of natural antibiotics for plant extracts is timely, and since a considerable amount of antimicrobial substances are present in many natural plant extracts, many studies are being conducted to apply them to food corruption. Therefore, natural antibiotics, which can substitute synthetic antibiotics from natural products that are not resistant to the present, are becoming essential for livestock farms, and thus, the natural antibiotics should be developed very urgently.

Streptococcus ( S. pyogenes , S. zooepidemicus ) is a pathogenic strain that destroys blood red blood cells, causing meningitis, pneumonia, endocarditis, and muscle necrosis. It is the causative agent of bacterial diseases that leads to. Staphylococci ( S. aureus , S. epidermidis ) secrete toxins and cause various diseases in humans and animals, while they die by heating during cooking, but endotoxins are heat-resistant and cause food poisoning. It is a pathogenic strain located on the throat mucosa causing skin diseases such as abscesses and wound infections, osteoarthritis, pneumonia, sepsis, and toxin shock syndrome. Clostridium ( C. difficile , C. perfringens ) secretes toxins and causes a variety of bowel diseases, dementia and bowel cancer.

Cactis japonica Makino is a Berberidaceae plant and is used as a medicinal herb in the fall as it is sunk and dried in the sun. Ingredients include berberine, coptisine, epiberberine, etc.In herbal medicine and folk medicine, it is used for antibacterial, anti-inflammatory, gastrointestinal disease, hyperemia, eye disease, etc.黄連 解毒 湯) is prescribed for detoxification and anti-inflammatory.

Schizandra chinensis RUPRECHT) is a Magnoliaceae plant that blooms in June-July and ripens in September-October and becomes magenta. Schisandra chinensis has been used in traditional medicine to improve systemic weakness, mental and physical fatigue, bronchitis, bronchial asthma, nervous breakdown, hypotension, cardiac dysfunction, malnutrition ulcers and wounds, and to improve visual acuity in tea or summer. It is processed and used mainly in starch foods and Schisandra chinensis using pigments. The antimicrobial effect of Schisandra chinensis has also been reported (Korean Patent No. 10-0854567).

Phellodendron amurense Ruprecht ( Phellodendron amurense Ruprecht) is a plant of the Rutaceae, called Rhubarb and Rhubarb , peeled in the summer and dried in the sun for use as a medicinal herb. The components of the white and yellow are Berberine, Palmitine, Magnoflorine, Guanidine, Guellodine, Phellodendrine, Jateorrhizine, Obakunone and Candicein Candicine), and is used in folk medicine and herbal medicine for antibacterial, anti-inflammatory, astringent, and inflammatory diseases.

Cheonmun-dong (天 을) edible light edible roots are used as Jinhae, diuretic, tonic. Astronomical dong is sweet and bitter, has a strong temper, and works on menopause and nerves to stop the coughing, soaking down the fever and stop coughing.In pharmacological experiments, the ingredient is asparagine, sputum, coughing, anticancer, urinating Bet. Its main components include saponin, flavonoids, and asparagine (Astronomical and Environmental Characteristics of Astronomical Dongs, Herbal Medicines, 11 (3) 212 ~ 215, (2003)). In addition, the components extracted from cheonmun-dong has an antimicrobial effect when cultivating bean sprouts (Korean Patent No. 10-0415759), and antimicrobial activity against harmful bacteria in vivo (Korean Patent No. 10-2003-0068748) Known.

The antibacterial effect of each of the sulfur, sulfur, Schizandra chinensis and astronomical dong is known, but it has not been reported whether the mixed herbal medicine composed of these antibacterial action.

Accordingly, the present inventors have shown that the herbal extract mixture containing two or more selected from the group consisting of the extracts of Hwang, Hwangbaek, Schisandra chinensis and Astronomical dong exhibits an antimicrobial effect against the genus Streptococcus, Staphylococcus and Clostridium, The present invention was completed by confirming that it can be usefully used as a natural antibiotic of herbal ingredients.

SUMMARY OF THE INVENTION An object of the present invention is to provide a composition for inhibiting growth of harmful bacteria in vivo, intestines or wounds, which contains a mixture of herbal medicines containing two or more selected from the group consisting of rhubarb, baekbaek, schisandra chinensis and astronomical extract as an active ingredient. will be.

Another object of the present invention is to provide a composition for treating plant blight comprising the herbal extract as an active ingredient.

Another object of the present invention is to provide a healthy food for inhibiting growth of harmful bacteria in vivo, containing the herbal extract as an active ingredient.

Another object of the present invention is to provide an antibiotic containing the herbal drug extract as an active ingredient.

In order to achieve the above object, the present invention provides a composition for inhibiting harmful bacterium growth in vivo, containing as an active ingredient a herbal extract mixed extract containing at least two species selected from the group consisting of Hwang lotus, Hwangbaek, Schisandra chinensis and astronomical extract.

In another aspect, the present invention provides a composition for inhibiting intestinal harmful bacteria growth containing the herbal extract as an active ingredient.

In another aspect, the present invention provides a composition for inhibiting harmful bacterial growth of the wound site containing the herbal extract as an active ingredient.

In addition, the present invention provides a composition for treating plant blight containing the herbal extract as an active ingredient.

In addition, the present invention provides a healthy food for inhibiting growth of harmful bacteria in vivo, containing the herbal extract as an active ingredient.

In addition, the present invention provides an antibiotic containing the herbal drug extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for inhibiting harmful bacterium growth in vivo, containing as an active ingredient a herbal medicine mixed extract containing two or more selected from the group consisting of Huangyan, Hwangbaek, Schisandra chinensis and Astronomical Dong extract.

Extracts of the yellow lotus, yellow baek, Schisandra chinensis and Astronomical dong of the present invention

1) extracting dried yellow lotus, yellowish white, Schisandra chinensis and astronomical dong into powder, and then adding an extracting solvent to each extract;

2) cooling each extract of step 1) and then filtering; And

3) is preferably prepared by a manufacturing method comprising the step of concentrating each filtrate of step 2) and lyophilizing, but not always limited thereto.

In the above production method, the extraction solvent of step 1) is preferably water, alcohol or a mixture thereof, it is preferable to use C ₁ to C ₄ lower alcohol, the lower alcohol is ethanol or methanol It is preferable to use but not limited thereto. The extraction solvent is preferably extracted by adding 1 to 1 to 10 times the amount of dried sulfur lotus, yellowish white, Schizandra and astronomical dong, more preferably 3 to 5 times to extract the extract. In addition, the extraction temperature is preferably 80 ~ 120 ℃, more preferably 800 ℃ but is not limited thereto. In addition, the extraction time is preferably 1 to 10 hours, more preferably 1 hour is not limited thereto. In addition, the extraction number is preferably 1 to 4 times, but is not limited thereto.

In the above production method, the concentration of step 3) is preferably used as an evaporator but not limited thereto. The concentration is preferably concentrated at 20 ~ 60 ℃ and preferably concentrated at 20 ~ 40 ℃ but is not limited thereto. The freeze-drying is preferably using a lyophilizer, more preferably using a vibration freeze dryer, but is not limited thereto. The lyophilization is preferably dried at -50 ~ -100 ℃ and preferably at -70 ℃ is not limited thereto.

The inventors of the present invention to investigate the effect of the four herbal medicines mixed extracts, that is, herbal extracts containing Hwangryeon, Hwangbaek, Schisandra chinensis and Astronomical dong on the growth of harmful strains. Quantitatively mixed by weight as follows. The composition ratio of the extract for the preparation of the herbal medicine mixture extract of the present invention is 0.1 to 6 parts by weight, 0.1 to 6 parts by weight of sulfur white, 0.1 to 4 parts by weight of Schisandra chinensis, 0.1 to 4 parts by weight of cheonmun-dong, More preferably, 6 parts by weight, 1 to 6 parts by weight of sulfur white, 1 to 4 parts by weight of Schizandra chinensis, 1 to 4 parts by weight of astronomical copper, 3 parts by weight of sulfur, 3 parts by weight of sulfur, 2 parts by weight of Schizandra chinensis and 2 parts by weight of Cheonmun-dong desirable.

The harmful bacteria in vivo are S. pyogenes of S. pyogenes , S. zooepidemicus in Streptococcus, which is an enterotoxic strain ; Causing many bowel disease Clostridium genus C. D. Pickle (C. difficile), C. Lin pulp Jens (C. perfringens); And S. aureus and S. epidermidis of the genus Staphylococci causing various purulent diseases in the wound.

Examples in the present invention include S. pyogenes and S. zooepidemicus in Streptococcus, which is an enterotoxic strain ; S. aureus , S. epidermidis in the genus Staphylococci, which secrete toxins and cause various purulent diseases; And, causing a number of bowel disease Clostridium genus C. D. Pickles (C. difficile), C. Lin presence pulp (C. perfringens) in the target, the impact on the growth of the harmful strain of the herbal mixture extract of the present invention Was investigated. As a result, C. difficile and C. pulprinzen by Schisandra chinensis and S. aureus, S. aureus and S. epidermidith by S. japonicus, S. fiogenes and S. z. Epidemius It was confirmed that growth is inhibited by, yellow lotus and Schizandra (see Table 2). In addition, C. pulprinzen was obtained by the mixture of Rum + + Schisandra chinensis, Rum + Rye astronomical and Hwangsi + Hwangbaek, and S. aureus by S. aureus This is done by Schisandra + astronomical dong and Schisandra + yellowish white extract mixture, S. piogenes by S. aureus + Schisandra chinensis, S. aureus + Astronomical dong and Silla + yellow white extract mixture, It was confirmed that growth was inhibited by the extraction mixture (see Table 2). In addition, C. pulprinzen by the Hwangryeon + astronomical dong + Hwangbaek extract mixture, S. aureus by the Hwangryeon + Astronomical dong + Hwangbaek extract mixture, and S. epidermidis by Schisandra + astronomical + Hwangbaek extract mixture, S. pyogenes was found to inhibit growth by H. rye + astronomical dong + hwangbaek and omija + astronomical dong + yellow and white extract mixture, and S. jupitemicus was inhibited by the mixture of rhubarb + astronomical dong + yellow baek and Schisandra + astronomical dong + yellow white extract mixture. (See Table 2). In addition, it was confirmed that growth of the six strains was inhibited by the extract mixture of Hwangyeon + Astronomical + Hwangbaek + Schisandra chinensis (1: 1: 1: 1) (see Table 2). The effect of the mixed extract prepared in a ratio of 3: 3: 2 was found to be the best (see Table 3 and Figure 1).

In addition, in another embodiment carried out in the present invention, by controlling the mixed extract of the herbal medicine of the present invention, the strains of the genus Clostridial harmful strains in the intestine were excellently controlled (see Figs. 2 and 3). In addition, the herbal extracts of the present invention, in vitro experiments on wounded rats to produce ① pus to swell the wound, secrete toxins, causing various diseases in humans and animals and staphylococci causing food poisoning Aureus strains; ② wounds ooze of the skin and causes skin diseases, osteoarthritis, pneumonia, sepsis, toxic shock syndrome, such as abscesses, wound infections, as pathogenic strains of merchandise to the throat mucosa, corneal ulcer, star, causing the disease to flounder Phycocosy Epidermidis Strains (epidermal staphylococci); ③ Streptococcus , a pathogenic strain that destroys red blood cells and causes meningitis, pneumonia, endocarditis, and muscle necrosis Piogenes strain; And (4) Bacterial Streptococcus , which causes death by infecting people, cattle, chickens, deer, etc. Jupitemicus Inhibition of the growth of the strain resulted in much faster healing (see FIGS. 4-7).

In vivo harmful bacterium growth inhibitory composition of the present invention preferably comprises 0.1 to 50% by weight of the herbal extracts with respect to the total weight of the composition is not limited thereto. The composition containing the herbal drug extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the preparation of the composition.

Pharmaceutical dosage forms of the in vivo harmful bacterium growth inhibitory composition of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds, as well as in a suitable collection. .

The compositions of the present invention can be used in the form of powders, granules, tablets, capsules, soft capsules, pills, granules, suspensions, emulsions, syrups, aerosols and the like oral formulations, external preparations, suppositories, and sterile injectable solutions. . Carriers, excipients and diluents that may be included in the composition containing the herbal mixture extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose, and the like in the herbal extract mixture. (sucrose), lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories, injections. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the in vivo harmful bacterium growth inhibitory composition of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the drug form, the route of administration, and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the raw drug mixture extract of the present invention is preferably administered at 0.0001 to 1000 mg / kg, preferably 0.001 to 100 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The harmful bacterium growth inhibitory composition of the present invention may be administered to mammals such as mice, mice, livestock, and humans by various routes. All modes of administration can be expected, for example, oral or parenteral, and may be externally administered, intraperitoneal, rectal, subcutaneous, intravenous, intramuscular or intrathoracic. It is preferable to select the injection method.

Herbal medicine mixture extract of the present invention is a composition that can be used with confidence even when taken for a long time because there is little toxicity and side effects.

In another aspect, the present invention provides a composition for inhibiting intestinal harmful bacteria growth containing the herbal extract as an active ingredient.

The intestinal harmful bacteria include S. pyogenes and S. zooepidemicus in Streptococcus, which are enterotoxic bacteria ; And, a disease that causes multiple copies of the genus Clostridium C. D. Pickle (C. difficile) and Jens C. Lin pulp (C. perfringens).

The herbal extracts can be usefully used as a composition for inhibiting the growth of harmful bacteria in the intestine because it has excellent growth inhibitory ability against intestinal harmful strains.

In another aspect, the present invention provides a composition for inhibiting harmful bacterial growth of the wound site containing the herbal extract as an active ingredient.

The harmful bacterium of the wound includes S. aureus and S. epidermidis of the genus Staphylococcus which causes various purulent diseases in the wound.

In addition, the composition of the present invention may be administered orally or parenterally, and when parenteral administration is selected by external skin or intraperitoneal injection, rectal injection, subcutaneous injection, intravenous injection, intramuscular injection or intrathoracic injection injection method. Preferably, most preferably, it is used for external skin.

Since the herbal extract extract is excellent in the ability to inhibit the growth of harmful strains on the wound site can be usefully used as a composition for inhibiting growth of harmful bacteria on the wound site.

In addition, the present invention provides a composition for treating plant blight containing the herbal extract as an active ingredient.

In addition, in the examples performed in the present invention, the herbal extract of the present invention was found to control nematodes in a concentration-dependent manner (see FIG. 8). Thus, the herbal extracts of the present invention can be usefully used as a composition for treating plant blight because it has an excellent ability to control nematodes that cause plant blight on wounds.

In addition, the present invention provides a healthy food for inhibiting growth of harmful bacteria in vivo, containing the herbal extract as an active ingredient.

Since the herbal extracts have excellent growth inhibitory ability against harmful strains of intestinal harmful strains and wounds, the composition of the present invention containing the herbal extracts as an active ingredient may be usefully used as a health food for inhibiting harmful bacteria growth in vivo. Can be.

The herbal mixture extract of the present invention may be added to a food as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). Generally, in the preparation of food or beverages, the composition of the present invention is added at 0.2 to 20% by weight, preferably 0.24 to 10% by weight relative to the raw materials. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range. It is certainly.

There is no particular limitation on the kind of food. Examples of foods to which the above-mentioned substances may be added include dairy products including drinks, meat, sausages, breads, biscuits, rice cakes, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, gums and ice cream, various soups, Beverages, alcoholic beverages, vitamin complexes, and the like, and include all healthy foods in the conventional sense.

In addition, the present invention provides an antibiotic containing the herbal drug extract as an active ingredient.

Since the herbal extracts have excellent growth inhibitory ability against harmful strains of intestinal harmful strains and wounds, the composition of the present invention containing the herbal extracts as an active ingredient may be usefully used as an antibiotic.

The herbal medicine extract of the present invention may be included in an amount of 1 to 100 parts by weight, preferably 50 to 100 parts by weight, and further added to one or more pharmaceutically acceptable carriers as a medicament. It can manufacture. The carrier may include, but is not limited to, saline, buffered saline, water, glycerol and ethanol, and any suitable agent known in the art (Remingtons's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA) may be used. .

The herbal extracts of the present invention can be used to provide natural antibiotics that can control bacteria harmful to the living body without side effects or resistance, and can be used as an agent for treating plant blight caused by wound healing and nematodes. .

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example 1 Noxious Strains and Culture Methods

<1-1> Streptococcus  Cultivation of the genus

Peptone 10.00 g, meat extract (Meat extract) 8.00 g, yeast extract (Yeast extract) 3.00 g, KH 2 PO 4 2.50 g, K 2 HPO 4 2.50 g, ㎎SO 4 · 7H 2 O 0.20 g, MnSO 4 · 4 0.05 g of H ₂ O, 5.00 g of Glucose, 5.00 g of Lactose, 0.50 g of Cysteine-HCl · H ₂ O, 1000.00 mL of distilled water, pH 6.8, 5% BSA after sterilization bovine serum), S. pyogenes (KCTC 3096) and S. zooepidemicus ( S. zooepidemicus : KCTC 3318) in Streptococcus, an enterotoxic strain, were cultured. 100 ml of the culture solution was added to a 250 ml flask and incubated at 37 ° C. with 150 rpm.

<1-2> Staphylococci  Cultivation of the genus

0.5 g of KH ₂ PO ₄ , 2.0 mg of NiCl ₂ · 6 H ₂ O, 10.0 ml of trace elements, 30.0 g of sulfur, powdered, 1.0 g of yeast extract (Difco), peptone (Bacto, Difco) 5.0 g, Resazurin 1.0 mg, 1000.0 ml of water, S. aureus ( S. aureus : KCTC 1928), S. epidermis in Staphylococcus, which secrete toxins and cause various purulent diseases Dummy ( S. epidermidis : KCTC 1917) was incubated. 100 ml of the culture solution was added to a 250 ml flask and incubated at 37 ° C. with 150 rpm.

<1-3> Culture in Clostridium

5.0 g of pancreatic digest of casein of casein, 5.0 g of protease peptone No3, 10.0 g of beef extract, 3.0 g of yeast extract, 5.0 g of glucose, 5.0 g of soluble starch ( Reinforced Clostridial Medium adjusted to pH 6.8 prepared with 1.0 g of soluble starch, 0.5 g of cysteine HCl, 3.0 g of sodium acetate, 3.0 g of agar, 1000.0 ml of purified water In the medium, C. difficile ( C. difficile : KCTC 2009) and C. perfringens (KCTC 3269) in Clostridium causing various intestinal diseases were cultured. 100 ml of the culture solution was added to a 250 ml flask and incubated at 37 ° C. with 150 rpm.

< Example  2> goldthread , Yellowing, oh Mija  And Astronomical building  Preparation of Extract

<2-1> Preparation of Water Extract

300 g of rhubarb, yellowish white, Schisandra chinensis and astronomical dong were taken, and each was put in a reflux flask, and 1,500 ml of water was added thereto, followed by heating under reflux for 1 hour. The extract was filtered, concentrated to 500 ml using an evaporator, and lyophilized to obtain 30 g of a powder (yield 10%).

Hwangnyeon, Hwangbaek, Schisandra chinensis and Astronomical dong consist of Dongbobogam, Jeungpang and medicinal herbs, the composition and application of herbal remedies, and prescription Chinese medicine. It was selected based on what is described as having astringent, anti-inflammatory and antimicrobial activity from ancient prescriptions in various books such as 圖 用 漢 方 處方, modern herbal lecture, and modern biopharmaceutical. Used.

<2-2> Preparation of Ethanol Extract

Except for using ethanol instead of water as the extraction solvent was extracted in the same manner as the extraction method of Example <1-1> to obtain a powder (yield 10%).

<2-3> Preparation of Methanol Extract

Except for using methanol instead of water as the extraction solvent, the extraction was carried out in the same manner as in the extraction method of Example <1-1> to obtain a powder (yield 10%).

< Example  3> goldthread , Yellowing, oh Mija  And astronomical agreement Herbal medicine  Preparation of Extraction Mixture

<3-1> Preparation of Extract Mixture of Two Herbs

To prepare a mixed solution of the two combinations of the extract of Hwangyeon, Hwangbaek, Schisandra chinensis and Cheonmundong 1: 1.

<3-2> Preparation of Extract Mixture of Three Herbal Medicines

Three mixtures of the extracts of Hwangnyeon, Hwangbaek, Schisandra chinensis and Astronomical dong were prepared 1: 1: 1.

<3-3> Preparation of Extract Mixture of Four Herbs

To prepare a mixed solution of the combination of the extract of Hwangyeon, Hwangbaek, Schisandra chinensis and Cheonmundong 1: 1: 1: 1.

<3-4> Preparation of Extract Mixture of Optimum Ratio of Four Herbs

The mixture was prepared by combining the extracts of Hwangnyeon, Hwangbaek, Schisandra chinensis and Astronomical dong in the ratio as shown in Table 1 below.

Prescription group goldthread Yellow white Schisandra Astronomical building B1 One One One One B2 2 One One One B3 One 2 One One B4 2 2 One One B5 3 2 One One B6 2 3 2 One B7 3 3 2 2

< Experimental Example  1> Control of each strain from one type of extract

The antimicrobial effect of the extract prepared in Example 2 for the harmful strains of Example 1 was confirmed by the Disk method. At this time, a positive control group (50 µg / ml tetracycline antibiotic) was placed in the center of the disk. Then, the diameter (mm) of the disc (Clear zone) in which bacteria do not grow was measured. Larger diameters indicate that bacteria do not grow well.

As a result, as shown in Table 2, C. difficle and C. pulpjensen were obtained by Schisandra chinensis and Rhus,

S. aureus and S. epidermidis,

S. piogenes and S. zhu epidemius confirmed that growth was inhibited by astronomical dong, rhubarb and schizandra.

< Experimental Example  2> Control of each strain from two kinds of extraction mixture

The antimicrobial effect of the extract mixture prepared in Example 3-1 for the harmful strains of Example 1 was confirmed by the Disk method. At this time, a positive control group (50 µg / ml tetracycline antibiotic) was placed in the center of the disk.

As a result, as shown in Table 2, C. pulprinzeneun by the extract mixture of rye + + Schisandra chinensis, sulfur + astronomical dong and sulfur + baekbaek,

S. aureus is characterized by the extract of Hunan + Schisandra chinensis, Hunan + Astronomical Dong, and Hunan + Huang White.

S. epidermidis was prepared by the Schizandra chinensis + astronomical dong and Schizandra chinensis extract mixture,

S. piogenes is produced by the extract of Hunan + Schisandra chinensis, Hunan + Astronomical Dong, and Huang + Huang White.

S. Jupitemicus was confirmed that the growth is inhibited by the extract mixture of Hwangyeon + Schisandra chinensis and Hwangyeon + Astronomical Copper.

< Experimental Example  3> Control of each strain from three kinds of extraction mixture

The antimicrobial effect of the extract mixture prepared in Example 3-2 for the harmful strains of Example 1 was confirmed by the Disk method. At this time, a positive control group (50 µg / ml tetracycline antibiotic) was placed in the center of the disk.

As a result, as shown in Table 2, C. pulprinzeneun by the sulfur mixture + astronomical dong + yellow white extract mixture,

S. aureus is characterized by extracting the mixture of rhubarb + astronomical tract + yellow white,

S. epidermidis was prepared by the Schizandra chinensis + Astronomical + Yellow-white extract mixture.

S. piogenes is prepared by extracting Hwangryeon + Astronomical Dong + Hwangbaek and Schisandra + Astronomical Dong + Yellow White

S. Jupitemicus was confirmed that growth is inhibited by the mixture of Hwang Ryun + Astronomical Dong + Yellow White and Schisandra + Astronomical Dong + Yellow White Extract.

< Experimental Example  4> Control of each strain from four kinds of extraction mixture

The antimicrobial effect of the extract mixture prepared in Example 3-3 for the harmful strains of Example 1 was confirmed by the Disk method.

As a result, as shown in Table 2, it was confirmed that growth was inhibited by the extract of Hwangryeon + Astronomical + Hwangbaek + Schisandra chinensis (1: 1: 1: 1) for the six strains.

Example goldthread Yellow white Schisandra Astronomical building Depickle Pearl Prince Aureus Epidermidis Piogenes Jupitemicus 2-1 One - 7 8 7 9 5 One - - - - - - One 4 5 - - 4 3 One - - - - 6 - 2-2 One - 6 9 7 8 4 One - - - - - - One 5 5 - - 5 4 One - - - - 7 - 2-3 One - 8 8 6 8 5 One - - - - - - One 5 5 - - 7 4 One - - - - 7 - 3-1 One One - 10 14 - 10 - One One - 11 14 - 12 7 One One - 9 14 - 11 7 One One - - - 5 - - One One - - - - - - One One - - - 8 - - 3-2 One One One - - - - - - One One One - 8 17 - 16 9 One One One - - - - - - One One One - - - 10 14 9 3-3 One One One One 9 22 19 14 19 12

< Experimental Example  5> By optimum mixing ratio Control of each strain

The antimicrobial effect of the extract mixture prepared in Example 3-4 for the harmful strains of Example 1 was confirmed by the Disk method.

As a result, as shown in Table 3 and Figure 1 it was shown that the best effect of the mixed extract (B7) prepared in the ratio of yellow: yellow white: Schisandra chinensis: astronomical dong = 3: 3: 2: 2 :.

Prescription group Depickle Pearl Prince Aureus Epidermidis Piogenes Jupitemicus B1 15 21 17 12 19 12 B2 13 27 20 12 19 12 B3 15 21 10 17 17 15 B4 13 25 17 15 15 14 B5 12 26 17 16 21 13 B6 14 23 13 16 18 14 B7 15 27 21 17 21 16

< Experimental Example  6> By herbal extract In vivo Clostridium  Control of genus strains

Eight male or female mice, 4 weeks of age, were purified for 1 week and were bred for 1 week while 100 μl / 200 g (mouse weight) of 1% crude drug mixture extract (0.2% -sucrosevh) was administered orally once daily. After one week, the excreta was obtained and pretreated with a FISH kit, which was then observed with a fluorescence microscope.

As a result, as shown in Figure 2 (male) and Figure 3 (female) significantly less harmful strains than the control group, by the herbal extract mixed extract of the present invention, a clot tree that causes intestinal inflammation, dementia, intestinal cancer, etc. in vivo It was confirmed that excellent control of the strain in the medium.

< Experimental Example  7> By herbal extract In vivo  Control of Harmful Strains in Trauma

① Staphylococcus that produces pus to break wounds, secretes toxins, causes various diseases in humans and animals, and causes food poisoning Aureus strains;

② wounds ooze of the skin and causes skin diseases, osteoarthritis, pneumonia, sepsis, toxic shock syndrome, such as abscesses, wound infections, as pathogenic strains of merchandise to the throat mucosa, causing illness in corneal ulcers, flounder Staphylococcus Kosi Epidermidis Strains (epidermal staphylococci);

③ Streptococcus , a pathogenic strain that destroys red blood cells and causes meningitis, pneumonia, endocarditis, and muscle necrosis Piogenes strain; And,

④ Bacterial Streptococcus Jupitemicus , which causes humans, cows, chickens, deer, etc. to cause death Strain

The wounds of the S.D. series rats were applied and observed for healing.

As a result, as shown in Figures 4 to 7, the group to which the herbal drug mixture extract of the present invention is applied shows a much faster healing as time passes, so that the herbal drug extract is well controlled staphylococcus aureus strain. Confirmed.

< Experimental Example  8> by herbal extract Nematode  salvation

After the nematodes, which cause plant blight, were placed on the plate, it was confirmed that the nematodes were controlled in a concentration-dependent manner when 0.01%, 0.5%, and 1.0% of the herbal extracts were administered at different concentrations (FIG. 8).

1 is a view showing the antimicrobial activity by the integrated prescription of the extraction mixture.

2 is a diagram showing the antimicrobial activity by the optimum mixing ratio of the extraction mixture.

3 is a diagram showing the number of bacteria from strains of Clostridium from the feces of male rats treated with the extract mixture of the present invention.

Figure 4 is a diagram measuring the number of bacteria from strains of Clostridium from the feces of female rats treated with the extract mixture of the present invention.

Figure 5 is a diagram showing the healing process of rats treated with the extract mixture of the present invention from the Staphylococcus aureus strain.

Figure 6 is a diagram showing the process of healing mice treated with the extract mixture of the present invention from the Staphylococcus epidermidis strain.

Figure 7 is a diagram showing the healing process of rats treated with the extract mixture of the present invention from Streptococcus piogenes strain.

Figure 8 is a diagram showing the healing process of the rats treated with the extract mixture of the present invention from the Streptococcus main epidemius strain.

Figure 13 shows the control activity against nematodes from the extraction mixture.

Claims (14)

As a composition for inhibiting harmful bacterium growth in vivo, containing as an active ingredient a herbal medicine mixed extract containing two or more selected from the group consisting of Hwang Ryun, Hwangbaek, Schisandra chinensis and Astronomical Dong extract, In vivo harmful bacteria include S. pyogenes , S. zooepidemicus , C. difficile , C. perfringens , Staphylococcus aureus ( S. aureus ) and Staphylococcus epidermidis ( S. epidermidis ) composition for inhibiting harmful bacterial growth in vivo, characterized in that the selected. The composition of claim 1, wherein the extract is extracted with water, alcohol or a mixture thereof. The composition of claim 2, wherein the alcohol is a C ₁ to C ₄ lower alcohol. 4. The composition of claim 3, wherein the lower alcohol is ethanol or methanol. 5. The composition of claim 4, wherein the lower alcohol is ethanol. The composition of claim 1, wherein the composition ratio of the herbal medicine mixture extract is 0.1 to 6 parts by weight, 0.1 to 6 parts by weight of sulfur white, 0.1 to 4 parts by weight of Schisandra chinensis and 0.1 to 4 parts by weight of astronomical dong. The composition of claim 6, wherein the composition ratio of the herbal medicine mixture extract is 1 to 6 parts by weight, 1 to 6 parts by weight of sulfur white, 1 to 4 parts by weight of Schizandra chinensis and 1 to 4 parts by weight of astronomical dong. 8. The composition of claim 7, wherein the composition ratio of the herbal medicine mixture extract is 3 parts by weight of sulfur, 3 parts by weight of sulfur white, 2 parts by weight of Schisandra chinensis and 2 parts by weight of astronomical dong. As a composition for inhibiting intestinal harmful bacterium growth, containing as an active ingredient a herbal medicine mixed extract containing two or more selected from the group consisting of Hwangyeon, Hwangbaek, Schisandra chinensis and Cheonmundong extract, Intestinal harmful bacteria are S. pyogenes , S. zooepidemicus , C. difficile and C. perfringens . Compositions selected from the group consisting of. As a composition for inhibiting the growth of harmful bacteria in wounds, which contains a mixture of herbal medicines containing two or more selected from the group consisting of Hwangyeon, Hwangbaek, Schisandra chinensis and Astronomical dong extract as an active ingredient, The harmful bacterium of the wound is Staphylococcus aureus ( S. aureus ) or Staphylococcus epidermidis ( S. epidermidis ) composition, characterized in that. A composition for treating plant blight comprising as an active ingredient a herbal medicine mixed extract containing two or more selected from the group consisting of Hwang Ryun, Hwangbaek, Schisandra chinensis and Astronomical Dong extract. 12. A composition according to claim 11 having a nematode control ability. As a health food for inhibiting harmful bacterium growth in vivo, containing a mixture of herbal medicines containing two or more selected from the group consisting of Hwangyeon, Hwangbaek, Schisandra chinensis and Cheonmundong extract as an active ingredient, The harmful bacteria are Streptococcus piogenes ( S. pyogenes ), Streptococcus epidemicus ( S. zooepidemicus ), Clostridium difficile ( C. difficile ), Clostridium pulpingensis ( C. perfringens ), Health food characterized in that it is selected from the group consisting of Staphylococcus aureus ( S. aureus ) and Staphylococcus epidermidis . As antibiotics containing a mixture of herbal medicines containing two or more selected from the group consisting of Hwang Ryun, Hwangbaek, Schisandra chinensis and Astronomical extract as an active ingredient, S. pyogenes , S. zooepidemicus , C. difficile , C. perfringens , Staphylococci au Antibiotic, characterized in that to inhibit the growth of the harmful bacterium selected from the group consisting of S. aureus and S. epidermidis ( S. epidermidis ).

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