KR20100115404A - Parthenocissus tricuspidata planch extracts for killing insects thereof - Google Patents

Abstract

PURPOSE: An agent for suppressing acyl CoA:cholesterol acyl transferase activation or insecticide containing parthenocissus tricuspidata PLANCH extract is provided to suppress sterol metabolism with safety. CONSTITUTION: An agent for suppressing acyl CoA:cholesterol acyl transferase(ACAT) activation contains Parthenocissus tricuspidata PLANCH extract as an active ingredient. An insecticidal composition contains Parthenocissus tricuspidata PLANCH extract as an active ingredient. The extract is a crude extract, polar solvent soluble extract or non-polar solvent soluble extract.

Description

Insecticide composition of solvent extract of ivy {Parthenocissus tricuspidata PLANCH extracts for killing insects

The present invention relates to an activity inhibitor or insecticide composition of acyl CoA: cholesterol acyltransferase (hereinafter referred to as “ACAT”) containing an ivy extract as an active ingredient.

[Document 1] Trend Newspaper 2006.7.25

[Document 2] Pesticide Yearbook 2004

[Reference 3] Entomology, Pyeong-saeng, Ji Hyunsa, 1996

[Document 4] Science, Vol 156, No. 3782, 1637-1638, 1967

Document 5 Medicinal Research Reviews, 14 (3), 271-305, 1994

[6] Brecher . P and C. Chen, Biochimica. Biophysica. Acat., 617 , pp 458-471, 1980

[Document 7] The Korean Society of Applied Entomology Vol 29, No.3, 194-200, 1990

The nations of the world gathered together to discuss ways to reduce the production and use of dangerous substances that have been used indiscriminately so as to protect human survival, among which the nations of the world agreed to reduce global warming and environmental pollution. Reached. Korea also plans to expand the quality competitiveness of our agricultural products and to transform the quality of our agricultural products into sustainable eco-friendly agriculture by spreading eco-friendly agriculture throughout our farms for the next five years from 2006. Quality and safety, and the proportion of eco-friendly agricultural products, which is currently 4%, will increase to 10% by 2010, and the use of pesticides and chemical fertilizers will be reduced by 30% by 2010 and 40% by 2013 to sustainably sustain our entire agriculture. It plans to switch to agriculture (Training News, June 25, 2006.7).

Many researchers around the world have tried to develop environmentally friendly insecticides that can replace organophosphorus and organochlorine pesticides, but have yet to develop new mechanisms for safe pesticides to replace highly toxic pesticides. Therefore, it is difficult to keep the contents of the agreement on the reduction of pesticides around the world, and if there is no safe pesticide production and the amount of pesticides to be used in the near future is expected to be a difficult problem not only in agriculture but also in various industries related to food production. have.

The UR Agricultural Agreement is largely made up of the fields of market opening on tariffs and tariff reductions, domestic subsidies that define agricultural subsidies, and export subsidies. In the domestic subsidy sector, agricultural subsidies of each country are classified into amber boxes, which should be reduced during the implementation period, and green boxes, which are exempt from reduction obligations. Direct payments using production restrictions as conditions are classified as blue boxes and excluded from the reduction.

According to the recent analysis of the global pesticide-related market trend, 2004 statistics show that the global pesticide market is $ 28 billion, of which herbicides account for 46.6%, pesticides 27.7%, fungicides 20.8%, and other pesticides 4.9%. According to 2006 statistics, the domestic pesticide market totaled 1.6 trillion won, which is different from the global market by pesticides.In Korea, pesticides 23.9%, insecticides 37.0%, fungicides 35.5%, and other pesticides 3.6% Is occupying. Pesticides, therefore, are marketed at 760 billion won and are gradually increasing (Pesticide Yearbook 2004).

Insect paths in the insect body have insect skin and gates in order to kill insecticides.When insecticides enter body tissues reach the point of action, some are decomposed and detoxified, and others are activated and turned into more toxic substances. Accumulate in the engine and some are discharged. Even when the drug reaches the umbilical cord, not all of them are involved in the insecticidal action but are resisted by various factors in the body tissues when invading, so only a part of them reaches the site of action, causing changes in insect physiology and biochemical function. Indicates lethal action. Therefore, considering the mechanism of action of pesticides, the point of action of pesticides and their methods, and the metabolism that governs the amount of pesticides effective in insect bodies have important meanings. Currently used insecticides can be classified into neurotransmission inhibitors, energy production inhibitors, growth regulators and sex pheromone agents in terms of their mechanism of action, among which growth regulators can be classified into glaze hormone inhibitors and chitin biosynthesis inhibitors. Insect insects are suppressed by abnormal stimulation, excitement or abnormality of insects, and insecticides that exhibit the inhibitory action of acetylcholinesteres are enzymes that decompose acetylcholine, a neurochemical transporter by organophosphorus and carbamate compounds. Inhibiting the activity of acetylcholine esters, acetylcholine accumulates at synapses, causing abnormalities in neurotransmitter function, causing convulsions and paralysis, resulting in the death of insects. Growth regulators exhibit insecticidal effects due to the composition of insect epidermis and inhibitory effect on chitin biosynthesis, and include glaze hormone inhibitors and chitin biosynthesis inhibitors. Insecticides that invade insects are metabolized by various enzymes such as oxidation, reduction and hydrolysis. Take apart. However, some pesticides have markedly increased toxicity, as opposed to detoxification. This change is called activation, which is mostly caused by oxidation in pesticides. The skin of insects is called the exoskeleton, and unlike the skin of vertebrates, the structure and chemical composition, such as body maintenance and muscle support, are different in many ways. Insects molt for gradual growth, and biosynthesis of the epidermis is very important for physiological function. Insect skin is composed of epidermis and basal membrane, which is divided into outer and primary epidermis. Chitin is not present in vertebrates and is a major constituent of the insect epidermis. Inhibiting chitin biosynthesis by insecticides, which are anti-skin inhibitors, causes insects to molt and die. Sex pheromone attractants induce males to capture and kill male pheromone secreted by insect females. However, the current pheromone attractant has not been shown to be effective in the field packaging test yet. Conventional pesticides use mechanical emulsifiers to cause suffocation by covering the surface of the cartilage, but most of the pesticides currently used are predominantly those that act on enzymes of the nervous or energy generating systems that play a fundamental role in maintaining life. It is coming true. Recently, insecticides are being developed that act on insect-specific functions, such as inhibiting the biosynthesis of chitin, which forms the epidermal layer, or inhibiting the production of glaze hormones (Insectology, Pyeong-saeng, Ji Hyun-sa, 1996).

Many researchers have discovered the research on the physiology of insects in part, and the recent research trend is on the study of metabolic enzymes and receptors through molecular biological methods.

It is known that sterols are required for the production of cell membranes during the growth of insects, the wax component of the epidermal layer, and the transport of fat from the hemolymph. In fact, most insects use 22-dehydrocholesterol (22) instead of cholesterol in the process of using this sterol. -dehydrochoesterol) or 7-dehydroergosterol can be replaced and these compounds are entomologically referred to as replacement compounds. However, it is known that sterols, which are involved in the synthesis of molten hormone, are available only with cholesterol and not with alternative compounds (insectology, paramedical, gypsy, etc.).

Many researchers have found that research on physiology of insects is partly related to metabolic enzymes and receptors using molecular biological methods, but not much has been done on hormone transport or storage of sterols.

Most insects do not have the ability to synthesize sterols, so they are required as essential nutrients to be taken from outside. Most insects eat vegetable sterols and convert them into cholesterol in the body. Some carnivorous insects eat other insects to eat sterols. It may be appropriate. Cholesterol is essential for the synthesis of molten hormones and is involved in the formation of cell membranes with phospholipids and various physiological functions (Science, Vol 156, No 3782, 1637-1638, 1967).

On the other hand, in humans and livestock, acyl CoA: cholesterol acyltransferase is involved in the acylation of cholesterol, which leads to the absorption of cholesterol in the small intestine, the synthesis of very low density lipoproteins in the liver, and the fat cell and vascular lining. It is known that the enzyme is involved in the accumulation of acylated cholesterol, which is a storage type, in the atherosclerosis, and new drug development of metabolic mechanisms is underway.Acyl Coay: Cholesterol acyltransferase inhibitors are organically synthesized. Synthesized compounds of urea, amide, and phenolic compounds are predominant. Among them, there are drug candidates in the preclinical stage to be used as an atherosclerosis prophylactic agent after in vivo activity test, but none have been used in the clinic yet (Medicinal Research Reviews, 14 (3) 271-305, 1994). ).

Therefore, the present inventors have taken advantage of the mechanism of insecticidal activity of the larvae which inhibits sterol acylase, which is involved in storage type or migration, in the mechanism of sterol metabolism, focusing on the phenomenon that insects necessarily require sterols. In the present invention, a new enzyme inhibitory activity was searched for from ivy extract, a natural resource widely existing in nature, and the present invention was completed by confirming insecticidal activity in Chinese cabbage worm larvae.

In order to achieve the above object, the present invention provides an activity inhibitor of acyl CoA: cholesterol acyltransferase (acyl CoA: cholesterol acyltransferase) containing an ivy extract as an active ingredient.

The present invention also provides a pesticide composition containing the ivy extract as an active ingredient.

The present invention also provides a pesticidal method comprising the step of treating the insecticidal composition in an insecticide or habitat in an effective amount.

Extracts as defined herein are characterized in that the crude extract of ivy, polar solvent soluble extract or non-polar solvent soluble extract.

The crude extract as defined herein is a solvent selected from water including purified water, lower alcohols having 1 to 4 carbon atoms such as methanol, methanol, butanol, or a mixed solvent thereof, preferably a mixed solvent of water and methanol, more preferably 70 Contains extracts soluble in ~ 100% methanol.

Polar solvent soluble extracts as defined herein include water, lower alcohols having 1 to 4 carbon atoms, or mixed solvents thereof, preferably extracts soluble in water.

Non-polar solvent soluble extracts as defined herein include hexane, chloroform, dichloromethane or ethyl acetate, preferably hexane, chloroform or ethyl acetate, more preferably extracts soluble in chloroform.

The pesticide as defined herein is characterized by controlling larvae, mites or nematodes of pests.

The pest as defined herein is characterized in that it is selected from Chinese cabbage moth, tobacco germ moth and white fire moth.

The aphid as defined herein is characterized in that it is selected from peach or aphids, raspberry bearded aphids, and green or aphids.

The nematodes as defined herein are characterized in that they are selected from cucumber root-knot nematodes, rice ear nematodes and rice root nematodes.

The pesticidal method as defined herein is characterized by spraying or administering to an insect.

Hereinafter, the method for obtaining the ivy extract of the present invention will be described in detail.

The ivy crude extract of the present invention is washed with water and dried in the shade after washing the ivy collected in Eeuneun-dong, Daejeon Yuseong-gu, and then powdered with a pulverizer, the powdered ivy is added to the solvent to extract and filtered. A solvent selected from 1 to 10 times, preferably 2 to 5 times the volume (w / v) of water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably water and methanol 1 to 15 days, preferably 3 to 10 days, a mixture of a solvent, more preferably 70-100% methanol, hot water extraction, cold extraction, ultrasonic extraction, reflux cooling extraction, heating extraction, etc. Extraction method of, the extract of the ivy crude extract of the present invention can be obtained through a manufacturing process of extraction by filtration under reduced pressure and concentration.

In addition, the polar solvent or non-polar solvent soluble extract of the present invention is 1 to 10 times, preferably 1 to 5 times the volume of hexane, chloroform, ethyl by weight of the crude extract of ivy, preferably the methanol extract of ivy. The acetate and water may be fractionated sequentially to obtain a soluble extract of polar or nonpolar solvent of the ivy of the present invention.

The present invention provides an activity inhibitor of acyl coay: cholesterol acyl transferase containing the ivy extract obtained by the above production method as an active ingredient.

The present invention also provides a pesticide composition containing the ivy extract obtained by the above production method as an active ingredient.

The composition containing the ivy extract of the present invention as an active ingredient is agrochemically acceptable with itself or with a salt (an inorganic acid such as hydrochloric acid and sulfuric acid, or an organic acid such as p-toluenesulfonic acid, without addition of any other ingredients). Salt). However, the extracts of the present invention are usually mixed with solid carriers, liquid carriers, gas carriers or baits, or absorbed into basic materials such as porous ceramic plates or nonwovens, followed by the addition of surfactants and other auxiliaries, if necessary, This can be in various forms such as oil sprays, emulsifiable concentrates, wettable powders, flows, granules, dusts, aerosols, smokers (eg fogging), vaporizable formulations, smoking formulations, toxic attractants, anti-mite sheets or It may be formulated in a resin formulation.

Each of the above formulations may typically contain 0.01 to 95% by weight of one or more of the present extract as an active ingredient.

Solid carriers that can be used in the formulation include fine powders or granules of clay materials such as kaolin clay, diatomaceous earth, synthetic hydrated silicon oxide, bentonite, fuvasami clay and acid clay; Various talc, ceramic and other inorganic materials such as biotite, quartz, sulfur, activated carbon, calcium carbonate and hydrated silica; And chemical fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea and ammonium chloride.

Liquid carriers include water; Water such as methanol and methanol; Ketones such as acetone and methyl ethyl ketone; Aromatic hydrocarbons such as benzene, toluene, xylene, ethylbenzene and methylnaphthalene; Aliphatic hydrocarbons such as hexane, cyclohexane, kerosene and light oils; esters such as ethyl acetate and butyl acetate; Nitriles such as acetonitrile and isobutynitrile; Ethers such as diisopropylether and dioxane; Acid amides such as N, N-dimethylformamide and N, N-dimethylacetamide; Halogenated hydrocarbons such as dichloromethane, trichloroethane and carbon tetrachloride; Dimethyl sulfoxide; And vegetable oils such as soybean oil and cottonseed oil.

Gas carriers or propellants may include freon gas, butane gas, LPG (liquefied petroleum gas), dimethyl ether, and carbon dioxide.

Base materials for use in toxic handouts include, but are not limited to, handout materials such as grain powders, vegetable oils, sugars and crystalline celluloses; antioxidants such as dibutylhydroxytoluene and nordihydroguaiaretic acid; Preservatives such as dehydroacetic acid; Materials for preventing non-eating and eating, such as pepper powder; And attractant flavors such as cheese flavor and onion flavor.

Surfactants may include alkyl sulfates, alkyl sulfonates, alkyl arylsulfonates, alkyl aryl ethers and polyoxyethylene derivatives thereof, polyethylene glycol ethers, polyhydric water esters and sugar water derivatives.

Adjuvants such as adhesives or dispersants include casein, gelatin; Polysaccharides such as starch, gum arabic, cellulose derivatives and alginic acid; lignin derivatives, bentonite, sugar and synthetic water soluble polymers such as polyvinyl water, polyvinyl pyrrolidone and polyacrylic acid.

Stabilizers include PAT (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (2-tert-butyl-4-methoxyphenol and 3-tert-butyl- Mixtures of 4-methoxyphenol), vegetable oils, mineral oils, surfactants, fatty acids and esters thereof.

When the compositions are used as agricultural insecticides, acaricides or nematicides, their application is usually from 0.1 to 100 g per 10 acres. For emulsifiable concentrates, wettable powders, flows and other similar formulations used after dilution with water, their application concentrations usually range from 1 to 1,000 ppm. Application of granules, dust or other similar formulations is carried out as undiluted formulations. When the compounds of the present invention are used as acaricides or nematicides for the prevention of mastopathy, they may be diluted to a concentration of 0.1 to 500 ppm with water, or in the case of emulsifiable concentrates, wettable powders, flows or other similar formulations, or For oil sprays, aerosols, smokers, toxic handouts, anti-mite sheets or other similar formulations, the same applies. The amount and concentration of this application may vary depending on the type of formulation, time of application, location and method, type of pest, degree of damage and other factors, and is not limited to the above range, and may be increased or decreased.

When the composition is used as a mite for controlling parasites of domestic animals such as cattle and pigs, or pets such as cats and dogs, the composition or its salts are known veterinary methods such as tablets and capsules for systematic control. Solutions for soaking, boli, food incorporation, suppositories or injections; Or by spraying, injecting (pouring or dripping) an oily or aqueous solution, or by using shaped articles made of suitable shapes such as collars and ear tags (tags) for non-systemic control. In this case, the compound is usually applied in an amount of 1 to 100 mg per kg of host body weight.

The compositions may be used separately or simultaneously with other insecticides, nematicides, acaricides, bactericides, fungicides, herbicides, plant growth regulators, synergists, fertilizers, soil conditioners and / or animal feeds. Can be.

Ivy extract of the present invention can confirm the mechanism of insecticidal action by inhibiting the sterol metabolism that insects are required in the body of the insect larvae, and confirm that the inhibition of sterol metabolism mechanism is harmless to the killing, can use a highly toxic pesticide Insecticides containing harmful nematodes, such as indoor horticultural insecticides and greenhouses that are difficult to spray pesticides, and also confirmed the efficacy as an insecticide with excellent enzyme activity inhibitors and excellent safety insecticides larvae resistant to conventional insecticides, the composition is acyl coei : It can be usefully used as a cholesterol acyl transferase inhibitor or an insecticide composition.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are only for illustrating the present invention and do not limit the scope of the present invention.

Example 1. Preparation of Ivy Leaf Methanol Crude Extract

The ivy leaves of the present invention were collected from Eeun-dong, Yuseong-gu, Daejeon, and consulted with a plant taxonomy. The collected ivy leaves were washed with water, dried in the shade, and powdered with a grinder. 1000 g of powdered ivy was added to 5000 ml of methanol, followed by filtration through solvent extraction at room temperature for 7 days, and the filtrate was concentrated under reduced pressure to obtain ivy crude extract of the present invention (hereinafter referred to as "PE"). 150 g (yield: 15%) were obtained.

Example 2. Preparation of Hexane Soluble Extract

Suspended in 150 g of methanol extract of the ivy leaf prepared by the method of Example 1 in 1000 ml of water, and then put into a 5000 ml fraction funnel, and then added with 1000 ml of hexane and shaken extract, fractions were hexane soluble Fractions and water soluble fractions were obtained, and the hexane soluble fraction layer was concentrated under reduced pressure to obtain 6 g of hexane soluble extract (hereinafter referred to as "PE-H") (extraction rate: 4%).

Example 3. Preparation of Chloroform Soluble Extract

1,000 ml of chloroform was added to the water-soluble fraction obtained in Example 2, followed by extraction, followed by fractionation to obtain a chloroform-soluble fraction and a water-soluble fraction. The chloroform-soluble fraction layer was concentrated under reduced pressure to obtain a chloroform-soluble extract (hereinafter, "PE-C"). 3 g (extraction rate: 2%) was obtained.

Example 4 Preparation of Ethyl Acetate Soluble Extract

1,000 ml of ethyl acetate was added to the water-soluble fraction obtained in Example 3, followed by shaking extraction to obtain an ethyl acetate soluble fraction and a water-soluble fraction. The ethyl acetate soluble fraction was concentrated under reduced pressure to obtain an ethyl acetate soluble extract (hereinafter, " 2 g (extraction rate: 1%) were obtained.

Example 5. Preparation of Water Soluble Extract

The remaining water-soluble fractions except for the ethyl acetate soluble fraction layer obtained by the method of Example 4 were concentrated and reduced pressure filtered to obtain 5 g of a water-soluble extract (hereinafter referred to as "PE-W") (extraction rate: 3.3%). It was.

Experimental Example 1. ACAT (Enzyme Inhibition) Activity Experiment

Insecticidal effect of the ivy extract (PE, PE-H, PE-C, PE-EA, PE-W) of the present invention is to inhibit the action of acyl coay: cholesterol acyl transferase (hereinafter referred to as "ACAT") The activity of this substance was measured by slightly modifying the Brecher method (Brecher. P and C. Chen, Biochimica. Biophysica. Acat., 617 , pp458 ~ 471, 1980).

The method uses a microsome partially purified from the liver as an ACAT active enzyme source, and reacts cholesterol and radioactively labeled oleoyl Co-A (Co-A) as a substrate. The degree of reaction was measured by the amount of radioactivity contained in the ester (cholesterol ester). Specifically, Triton WR-1339 dissolved in acetone and Tritone WR-1339 dissolved in acetone were suspended in water, and acetone was removed with nitrogen gas, and then K-phosphate buffer solution (pH 7.4, final concentration 0.1 M) was added. In order to stabilize the enzyme reaction, bovine serum albumin was added to a final concentration of 30 μM, and the sample dissolved in DMSO or MeOH was appropriately added and preliminarily reacted at 37 ° C. for 30 minutes. After the preliminary reaction, the reaction was performed by adding Oleoyl-Coenzyme A ([1- ¹⁴ C] oleoyl-Coenzyme A) as 0.04 μCi and reacting at 37 ° C. for 30 minutes. After completion of the reaction, 1 ml of isopropanol-heptane was added to stop the reaction. Then, 0.6 ml of n -heptane and 0.4 ml of KPB bufferf were added thereto, mixed well, and left for 2 minutes. Once separated, 200 μl of the supernatant was taken and placed in a scintillation vial. 4 ml of scintillation cocktail (Lipoluma, Lumac Co.) was added to the solution to measure the amount of cholesteryl oleate produced at the scintillation counter (Packard Delta-200). Activity was calculated according to the following formula (1).

Inhibitory activity (%) = [1-(T-B / C-B)] * 100

T: Put the sample in the enzyme reaction solution cpm value of the test group,

C: cpm value of the control group without the sample in the enzyme reaction solution,

B: cpm value of the control group without the enzyme source.

In this blank test, the inhibitory activity was calculated by correcting the test value without the enzyme source and the test value without the sample.

As a result, as shown in Figure 1, ACAT enzyme inhibition of the ivy crude extract (PE) of the present invention was determined to be 49.29% enzyme inhibition when 100 ug / ml, ivy soluble extract extract (PE-H ), Chloroform soluble extract (PE-C), ethyl acetate soluble extract (PE-EA), and water soluble extract (PE-W) were 66.89%, 67.30%, and 9.69 when treated at 100 ug / ml, respectively. %, 46.94%. In ivy crude extract (PE) and chloroform soluble extract (PE-C), it was confirmed that acyl coay: cholesterol acyl transferase enzyme inhibitory activity was the best.

Experimental Example 2. Activity test on Chinese cabbage moth larvae

In order to confirm the insecticidal effect of the Chinese cabbage moth larvae of the ivy crude extract (PE) of the present invention exhibited an excellent inhibitory activity against the acyl coei: cholesteryl acyl transferase (ACAT) enzyme in Experimental Example 1 The experiment was performed according to the Korean Society of Applied Entomology vol29, No3, 194-200, 1990.

The test insect used to confirm the insecticidal effect was Plutella xylostella L. The insect pest experiment was conducted in September 2006 at the Department of Agricultural Biology, Chungbuk National University, Cheongju, Chungcheongbuk-do, Korea.

Accurately weigh the ivy crude extract (PE) of the present invention having the ACAT inhibitory activity identified in Experimental Example 1, dissolve an appropriate amount in acetone, and dilute and treat sequentially with Triton X-100 100 ppm aqueous solution 9 times. The active search substance solution was prepared.

The cabbage leaf moth caterpillars used in this experiment were cut to a cabbage leaf in a uniform growth state with a leaf disk (3.0 cm in diameter), soaked in the prepared active search material solution sufficiently for 30 seconds, and then taken out and dried in a hood for 60 minutes. . Place the leaf with active search substance on Petri dish (55 × 20 mm) covered with filter paper moistened with distilled water, and move the larvae with a soft brush to avoid damaging the larvae. Inoculation. The cabbage moth larvae treated with active screening material were bred in constant temperature room (25 ± 1 ℃, relative humidity 40-45%, 16L: 8D) and tested for insecticides at 24 and 48 hours. The control group treated with Triton X-100 100 ppm aqueous solution 9 times in acetone 10% solution except the treated extract was treated in the same manner as the active screening treatment. The activity search experiment was performed in three repetitions and the results are shown in FIG. 2 and Table 1. FIG.

As a result of the experiment, as shown in Figure 2 and Table 1, the ivy crude extract obtained by the method of Example 1 was treated to 1, 10 and 100 ppm by the Chinese cabbage moth larvae and measured the degree of pesticidal 4 days at intervals of 1 day. When compared with the control group, persistent insecticidal phenomena appeared by the treatment of crude ivy extract (PE), the insecticidal effect was confirmed that the effect is excellent in concentration and time dependent.

sample density
ppm repeat Population Insecticide 4Day Insecticide Rate (%) 1Day 2Day 3day 4day Ivy
Crude extract 1000 One 10 2 10 10 10 100.0 2 10 3 10 10 10 3 10 2 10 10 10 100 One 10 4 6 8 10 96.7 2 10 2 5 10 10 3 10 2 7 7 9 10 One 10 3 4 7 7 80.0 2 10 3 5 7 7 3 10 2 8 10 10 One One 10 0 5 5 9 73.3 2 10 2 5 7 7 3 10 2 6 6 6 Control One 10 0 0 One 2 10.0 2 10 0 0 0 0 3 10 0 0 0 One

[National R & D project supporting this invention]

[Task unique number] 107048032SB010

[Department name] Agriculture and Forestry Technology Management Center

[Name of Research Project] Rural Development Project

[Project title] Development of new concept insecticide material regulation of sterol metabolism for growth and stripping of feeding insects

[Host] Korea University

[Research Period] May 30, 2008 ~ May 29, 2009

1 is a diagram measuring the inhibitory effect of acyl coai acyl transferase of ivy extract (PE, PE-H, PE-C, PE-EA, PE-W),

Figure 2 is a diagram measuring the insecticidal effect of the cabbage moth larvae of ivy crude extract (PE).

Claims (12)

An inhibitor of the activity of acyl coay: cholesterol acyl transferase (ACAT) containing ivy extract as an active ingredient. Insecticide composition containing ivy extract as an active ingredient. The composition of claim 2, wherein the insecticide controls larvae, mites or nematodes of the pest. 4. The composition of claim 3, wherein the pest is selected from Chinese cabbage moth, tobacco castor moth and white fire moth. 4. The composition of claim 3, wherein the mite is selected from peach or aphids, raspberry bearded aphids, and green or aphids. 4. The composition of claim 3, wherein the nematode is selected from cucumber root-knot nematodes, rice ear nematodes and rice root nematodes. The composition according to claim 2, wherein the extract is a crude extract of ivy, a polar solvent soluble extract or a nonpolar solvent soluble extract. 8. The composition of claim 7, wherein the crude extract is an extract available in a solvent selected from water including purified water, lower alcohols having 1 to 4 carbon atoms of methanol, ethanol, butanol, or a mixed solvent thereof. 8. The composition of claim 7, wherein the polar solvent soluble extract comprises an extract soluble in water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. 8. The composition of claim 7, wherein the nonpolar solvent soluble extract comprises an extract soluble in hexane, chloroform, dichloromethane or ethyl acetate. 3. A composition according to claim 2, characterized in that the composition comprises, without addition of any other ingredients, an agrochemically acceptable salt with itself or with an inorganic acid such as hydrochloric acid and sulfuric acid, or an organic acid such as p-toluenesulfonic acid. Composition. 3. The composition of claim 2, wherein the composition is an oil spray, emulsifiable concentrate, wettable powder, fluid, granule, dust, aerosol, smoker, vaporizable formulation, smoking formulation, toxic attractant, anti-mite sheet or resin formulation. A composition comprising water.

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