KR20100062308A - Inhibition of alcoholic hangover and gastric ulcer by sweet potato fermentation fractions - Google Patents

Abstract

PURPOSE: A purple sweet potato fermentation-filtered fraction is provided to remove anthocyanine and to enhance hangover relief and gastric ulcer treatment. CONSTITUTION: A method for preparing purple sweet potato fermentation-filtered fraction comprises: a step of fermenting purple sweet potato with Saccharomyces at 60-70°C for 42-54 hours; a step of filtering to obtain sweet potato fermentation; and a step of isolating anthocyanine and removing.

Description

Inhibition of alcoholic hangover and gastric ulcer by sweet potato fermentation fractions

The present invention is a sweet potato yeast fermentation filtrate (SPF) filtered after the fermentation of purple sweet potato at 60-70 ℃ 42-54 hours in Saccharomyces yeast strain, or the sweet potato fermentation filtrate separated anthocyanin from the fermentation filtrate The present invention relates to a method of using the extracted liquid component fraction as a hangover detoxifying substance for decreasing ethanol blood concentration and aldehyde blood concentration or as a gastric ulcer inhibitor.

A hangover is a phenomena experienced by people who have drunk until they get drunk, and it is a physical and mental symptom that is often not pleasant. The cause is known as a deficiency of nutrients (lack of blood sugar, minerals and vitamins) due to the toxicity, dehydration and absorption disorders of alcohol and its intermediate metabolite acetaldehyde. The degree of hangover varies greatly depending on individual variation (genetic literacy) and environmental conditions (nutrition, exercise, dehydration, health, etc.). Representative symptoms include thirst, vomiting, fatigue, dizziness, and headaches, and these factors may work in combination.

Alcohol is oxidized to acetaldehyde, known as the causative agent of hangover, by alcohol dehydrogenase (ADH) and coenzyme NAD + in the liver and decomposed to acetic acid by aldehyde dehydrogenase (ALDH) and NAD +.

Acetaldehyde is also oxidized by cytochrome P-450 type 2E1 (CYP2E1) and catalase. Acetaldehyde and acetic acid cause cytotoxicity, headache and abdominal pain, and dehydration due to osmotic pressure change through lipid peroxidation. In addition, peptic ulcers, one of the major diseases of modern people, indicate damage to gastric acid (gastric HCl) and pepsin submerged areas, which are normally covered by mucin secreted by mucosal cells. Therefore, gastric mucosal erosions and ulcers are caused by various causes such as gastric acid secretion, weakening or depletion of mucin layer against pepsin invasion, local blood circulation disorder, cell damage and inflammatory reaction.

Thus, the causative agents of gastric ulcers are drugs such as nonsteroidal anti-inflammatory drugs (NSAIDs) that inhibit cyclooxygenase (COX), an enzyme that produces PGs that strengthen the mucin layer and facilitate local blood circulation. Gastric acid secretion and retention, increased gastrointestinal motility and acetic acid accumulation, and bacterial infections such as Helicobacter pylori. In particular, high concentrations of alcohol cause congestion of the gastric mucosa, which causes direct bleeding and necrosis, as well as lipid peroxidation due to radical reactions.

Gastric ulcer drugs include hydrogen ion pump inhibitors that block acid secretion from wall cells, antacids, histamine receptors that promote acid secretion (H2-antagonists), PGs and derivatives of mucin layer enhancers, and Helicobacter pylori Antibiotics that can eradicate the disease are typical (Wallace and Granger, 1996; Neal, 2003).

Alcoholic hangovers and stomach ulcers are one of the most indispensable diseases in modern people, and many suffer from them. In particular, in addition to social stress, frequent intake of alcohol is accompanied by severe gastric ulcers and liver disease, and many medicines and functional foods are being sold to improve them. In other words, efforts are being made to lower alcohol blood levels by activating ADH and ALDH. However, there are few effective substances that show significant detoxification effects by activating enzymes in a short period of time. In particular, a gastric ulcer therapeutic agent such as pantoprazole, which is a hydrogen ion pump inhibitor, is effective for gastric ulcers caused by other causes, but is less effective in alcoholic gastric ulcers (Cao et al., 2004).

Therefore, attention is focused on antioxidants that can alleviate hangover and gastric ulcer symptoms such as heartburn by protecting the cells and tissues from radical reactions as well as promoting alcohol degradation in the stomach and liver. Polyphenols, the most abundant antioxidant in our diet, are receiving great attention from consumers and food manufacturers. Among the known polyphenols, anthocyanin is consumed more than 100 mg per day, which is much more than other substances, which is of particular interest due to the purple color present in many fruits, vegetables, flowers, such as grapes, strawberries, cherries, mulberries, cabbage, etc. The band is a water-soluble pigment.

Anthocyanins are flavonoids which are hydroxylated and methoxylated derivatives of phenyl-2-benzopyrylium or flavilium salts. Until recently, 17 natural anthocyanins and aglycones have been known, and although there are about 600 chemical structures, they are cyanidin, peonidin, pelagonidin, malvidin, and delfinidine. (delphinidin) and petunidin are six major components (Giusti and Wrolstad, 2003; Kong et al., 2003; Harada et al., 2004; Mazza, 2007).

Anthocyanins inhibit the action of angiotensin converting enzyme (ACE), which is involved in the cardiovascular diseases such as removal of free radicals that cause cellular aging, inhibition of mutations that cause cancer, hypertension, arteriosclerosis, and myocardial infarction, and blood Elimination of cholesterol, especially low density lipoprotein (LDL), which causes arteriosclerosis, and improvement of obesity, prevention and treatment of fatty liver, liver cirrhosis and alcoholic liver disease, enhancement of brain metabolism function by promoting blood circulation, prevention of constipation, and Vision improvement is known (Mazza, 2007). Recently, large amounts of anthocyanins were found in purple sweet potatoes (Harada et al., 2004), and anthocyanins in black tea were reported to inhibit the oxidation reaction due to alcohol intoxication (Luczaj and Skrzydlewska, 2004).

Therefore, the present invention judges that anthocyanin may be effective in improving hangover and gastric ulcer caused by excessive drinking through improving liver function, blood circulation, antioxidant activity, etc., and sweet potato fermentation filtrate of purple sweet potato containing large amount of anthocyanin. The present invention has been completed by measuring the efficacy of fractionation of purple sweet potato yeast fermentation product (SPF) by measuring the hangover and gastric ulcer improvement efficacy of SPF) and identifying the physical properties of the active ingredient.

The problem to be solved by the present invention is that anthocyanin is effective in improving hangover and stomach ulcer caused by excessive drinking through improving liver function, blood circulation, antioxidant activity, etc., and fermentation filtrate of purple sweet potato containing large amount of anthocyanin ( potato) Purple sweet potato yeast fermentation filtrate (SPF), ii) Purple sweet potato yeast fermentation filtrate extraction Anthocyanin fraction, ⅲ) Purple sweet potato yeast fermentation filtrate extraction To evaluate the efficacy of the improvement of the hangover and gastric ulcer by comparing and evaluating the efficacy of the fraction of purple sweet potato yeast fermentation filtrate (SPF) through the liquid component fraction.

In this case, the purple sweet potato yeast fermentation filtrate liquid phase fraction is separated into a water fraction layer, a methanol fraction layer and a butanol fraction layer, and the efficacy of each fraction layer is also intended to be measured separately.

An object of the present invention is to ferment the purple sweet potato in Saccharomyces yeast strain at 60-70 ℃ 42-54 hours, and then filtered sweet potato yeast fermentation filtrate (SPF), or sweet potato fermentation separated and removed anthocyanin from the fermentation filtrate The present invention provides a method of using the filtrate extract liquid component fraction as a hangover detoxifying substance for reducing ethanol blood concentration and aldehyde blood concentration.

Still another object of the present invention is to remove purple sweet potato with Saccharomyces yeast strain at 60-70 ° C. for 42-54 hours, and then remove and remove anthocyanin from the filtered sweet potato yeast fermentation filtrate (SPF) or the fermentation filtrate. Sweet Potato Fermentation Filtrate Extract The present invention provides a method of using a liquid component fraction as an alcoholic gastric ulcer inhibitor.

On the other hand, the alcoholic gastric ulcer inhibitor is characterized in that the butanol fraction material obtained by separating the liquid component fraction of sweet potato fermentation filtrate extracted from the fermentation filtrate separated into a water fraction layer, methanol fraction layer and butanol fraction layer.

In addition, hangover detoxification or alcoholic gastric ulcer inhibitors of the sweet potato yeast fermentation filtrate is characterized in that the substance other than anthocyanin.

The effect of the present invention is that anthocyanin is effective in improving hangover and gastric ulcer caused by excessive drinking through improving liver function, blood circulation, antioxidant activity, etc., and yeast fermentation filtrate of purple sweet potato containing large amount of anthocyanin (sweet 측정) Purple sweet potato yeast fermentation filtrate (SPF), ii) Purple sweet potato yeast fermentation filtrate Anthocyanin fraction, ⅲ) Purple sweet potato yeast fermentation filtrate By comparing and evaluating the efficacy according to the fraction of the purple sweet potato yeast fermentation filtrate (SPF) through the component fraction to provide a hangover and gastric ulcer improvement effect. In addition, the purple sweet potato yeast fermentation filtrate extract liquid component fraction is separated into a water fraction layer, methanol fraction layer and butanol fraction layer to provide the efficacy of each fraction layer.

Hereinafter, the present invention will be described in more detail.

Hangover Relief

After an oral dose of 3 mL / kg (20 mL to 15 mL / kg) of ethanol in normal animals, blood levels of 0.13% at 1 hour, 0.13% at 3 hours, and 0.10% at 5 hours were shown. It gradually decreased after reaching the peak at (Fig. 1). In contrast, the serum ethanol concentration of oral administration of 240 mg / kg (15% at 1.6 mL / kg) 30 minutes prior to ethanol administration was 0.10% at 1 hour, 0.11% at 3 hours, and 0.07% at 5 hours. This resulted in a 20-25% reduction in all time periods. Particularly, in the group pre-administered 750 mg / kg (15% at 5 mL / kg) of SPF, the ethanol concentration was 0.05-hour at 1 hour, 0.07% at 3 hours, and 0.05% at 5 hours. %, And SPF-liquid component mg / kg) showed a similar effect to SPF. In comparison, the same dose (750 mg / kg) of purified anthocyanin was ineffective. In particular, commercially available hut extract (HDE) also showed a relatively low effect.

On the other hand, blood acetaldehyde concentration was maintained in the body for a considerable period of time maintaining the trend of gradually increasing to an average of 2.50 ppm at 1 hour, 2.09 ppm at 3 hours, and 3.20 ppm at 5 hours after oral administration of ethanol (Fig. 2). Interestingly, when 240 mg / kg of SPF was administered 30 minutes before, an average increase of 3.72 ppm in 1 hour, 2.44 ppm in 3 hours, and 1.96 ppm in 5 hours showed an initial sharp increase, and after 5 hours, It quickly decreased to low levels. When 750 mg / kg SPF was administered, the rate rose to an average of 4.85 ppm at 1 hour, 4.46 ppm at 3 hours, and 2.42 ppm at 5 hours, then recovered rapidly and was lower than the control group.

In order to evaluate hangover detoxification effect, SPF was administered 1 hour after the ethanol concentration reached its highest level, and the blood concentration of ethanol decreased rapidly, about 2.8 times at 240 mg / kg and 3.1 times at 750 mg / kg. The fast dissipation rate was shown (FIG. 3). The SPF-liquid component also rapidly lost ethanol blood concentration to the level comparable to that of SPF, whereas the effect of anthocyanin was relatively weak. In addition, the efficacy of the commercially available HDE was very small. On the other hand, blood acetaldehyde concentration was also confirmed that the disappearance rate is faster than the control group in a dose-dependent manner by the SPF administration (Fig. 4).

Alcoholic Gastric Ulcer Inhibitory Effect

Male rats were orally administered with ethanol (3 mL / kg) of the stock solution, and after 1 hour, the stomach was extracted and observed. As a result, severe hemorrhagic gastric ulcer was confirmed (FIG. 5). This gastric mucosal injury was 82.3 ㅁ 10.4mm when converted into gastric ulcer index (Table 1).

On the other hand, when SPF was administered prior to ethanol administration, gastric ulcer was suppressed due to dose-dependence, and especially at 100 mg / kg or more, almost perfect effect was obtained. In particular, the palliative effect of gastric ulcer was shown to be the same level by the SPF-liquid component, showing an inhibitory effect of 48.0% at 30 mg / kg, and almost completely at 100 mg / kg or more. On the other hand, purified anthocyanin showed no effect at low doses of 30 mg / kg, and only 30.0% of low doses at high doses of 300 mg / kg.

From these test results, the liquid component identified as containing the gastric ulcer inhibitory active ingredient was partitioned into water, butanol and methanol layers. As a result of evaluating the gastric ulcer prevention effect on these fractions, the water fraction of 100 mg / kg and the methanol fraction showed the inhibitory effect of 42.4% and 32.3%, respectively, and the effect was relatively low (Fig. 6 and Table 2). In comparison, the 100 mg / kg butanol fraction showed a high effect of 74.7%, which was less than 92.9% of the high dose (300 mg / kg) SPF, but was the most effective of the three fractions.

In order to quantify the amount of bleeding associated with gastric ulcers, the leaked Evan's blue was eluted, and the water, butanol, and methanol fractions showed 34.4, 76.8, and 28.4% of inhibitory effects, respectively, indicating a tendency to closely match the gastric ulcer index. Indicated

Recently, as the problem of synthetic pigments is raised and the demand for natural pigments increases, interest in anthocyanins as functional natural pigments is increasing. This interest began with the discovery of anthocyanin's health-promoting functionality, and the results of epidemiological studies on the association between the high intake of anthocyanin-induced wine called "French Paradox" and the prevention of coronary and heart disease. (Renaud and De Lorgeril, 1992; Ramirez-Tortosa et al., 2001). Since then, numerous studies have revealed the benefits of anthocyanin for human health, and its typical effects include radical scavenging, inhibition of phospholipid oxidation, inhibition of platelet aggregation, anti-inflammatory action, reduction of capillary permeability and vulnerability, prevention of obesity, and prevention of liver ischemia. , Anticancer and cancer prevention, vision improvement, diabetes improvement (Jankowski et al., 2000; Cohen-Boulakia, 2000; Hagiwara et al., 2001; Harris et al., 2001), and these effects are known to be associated with the strong antioxidant power of anthocyanins. (Mazza, 2007), and especially have been reported to show an excellent antioxidant effect in the oxidation reaction due to alcoholism (Luczaj and Skrzydlewska, 2004). From these theoretical backgrounds and previous series of test results, we predicted the improvement effect of alcoholic hangover and gastric ulcer, which are known to be involved in radical reaction.

In the hangover prevention effect, the dose-dependent dose of ethanol decreased the blood concentration of ethanol, especially at the high dose of 750 mg / kg, which was significantly lower in the gastrointestinal tract than that of the first hour after ethanol administration at 3 hours. It is believed to have an absorption or metabolic promoting effect. This can be inferred from the fact that the absorption rate is very low when a relatively small amount of ethanol is administered in the preliminary test. Interestingly, the pretreatment of 240 mg / kg SPF resulted in a significant increase in acetaldehyde blood level in one hour and rapidly recovery despite the decrease in ethanol blood concentration. Especially at 750 mg / kg the initial rise was higher because the yeast fermentation activates ADH in the gastrointestinal tract and promotes the metabolism of acetaldehyde, thereby reducing the absorption of ethanol while increasing the absorption of acetaldehyde. Nevertheless, the acetaldehyde concentration, which had risen high, decreased very rapidly and was lower than that of the control at 5 hours.

This increase in the cleaning rate was also confirmed in the hangover detoxification effect. In other words, when SPF was administered 1 hour after the blood ethanol concentration was sufficiently high, the rate of ethanol excretion was significantly increased, and the acetaldehyde clearance rate was also proved to be excellent in preventing and detoxifying effects. Considering these trends in blood ethanol and acetaldehyde concentrations, SPF is believed to lower the absorption rate of ethanol and promote metabolism in the liver through enzymatic activation in the intestine. Interestingly, acetaldehyde produced in the intestine or liver has been shown to impede the absorption of alcohol (Kinoshita et al., 1996). The high concentration of acetaldehyde produced by high concentrations of acetaldehyde produced by ADF activated by SPF in the intestine leads to It is believed to increase temporarily and consequently decrease alcohol absorption.

Interestingly, the excellent hangover efficacy of SPF was seen at the same level in its liquid components, but was not reproduced by anthocyanins, which are widely known for their antioxidant effects. Therefore, it was inferred that there is a new hangover effective ingredient in the liquid component. In the control effect of alcoholic gastric ulcer, oral administration of 3 mL / kg of ethanol caused severe hemorrhagic ulcer, but was completely completely suppressed to 98% by 100 and 300 mg / kg of SPF or its liquid component. In contrast, purified anthocyanin did not show a great effect at the same dose, it was confirmed that the active ingredient is present in the liquid component in the improvement of gastric ulcer as well as hangover.

Furthermore, the water, butanol and methanol fractions of the SPF-liquid component were evaluated for gastric ulceration improvement. As a result, the most effective effect was confirmed in the butanol fraction, and it was estimated that the butanol fraction contained more active ingredients than the water and methanol fractions.

Taken together, the results showed that SPF administration significantly reduced blood alcohol concentration and increased detoxification effect of alcohol and acetaldehyde in hangover prevention effect. It was found to be due to other components present in the SPF-liquid component. In addition, the alcoholic gastric ulcer showed excellent efficacy in the SPF-liquid component, and the active ingredient appeared to be contained at a relatively high level in the butanol fraction. Thus, the butanol fractions of SPF, SPF-liquid and SPF-liquid components have demonstrated the possibility of developing new functional materials for improving hangover and gastric ulcer caused by excessive alcohol intake, and further separating the active ingredients from the butanol fraction. It is expected that better effective new materials can be obtained if the mechanism of action is identified.

The present invention will be described in more detail with reference to the following examples. However, these examples do not limit the scope of the present invention.

Experimental animal

Sprague-Dawley (SD) male 6-week-old rats were purchased from Orient Bio Co., Ltd. (Gapyeong, Gyeonggi-do), and were kept in cages and rations for 7 days. Animal weights at the start of the test were 220-240 g. Animals were reared in an environment in which temperature (23 W 2 o C), relative humidity (50 W 5%) and 12 hour contrast cycle (07:00 lit, 19:00 off) were controlled. The feed was freely fed solid food for experimental animals, the negative was filtered purified water. All animal experiments in this study were performed in accordance with the Center's Standard Operation Procedures (SOP) with the approval of the Institutional Animal Care and Use Committee (IACUC).

Test substance

Purple sweet potato grown in Hamyang-gun, Gyeongnam was fermented with yeast strain (Saccharomyces HM2104) at 60-70 ° C. for 48 hours and then filtered to obtain purple sweet potato fermentation filtrate (SPF). From this fermentation, only anthocyanins were purified by the stepwise Sepadex column fractionation method to obtain SPF-liquid components from which anthocyanins were completely removed. The liquid component was dried at 40 ° C. and then extracted three times with methanol: water (1: 1) and the methanol layer was dried to give a methanol fraction. The water layer was extracted again with butanol: water (1: 1) three times and the butanol layer was dried to obtain a butanol fraction. The remaining water layer was dried to obtain a water fraction and used in the experiment. Separately, Hovenia dulcis extract (HDE) was purchased from the tree of Life Co., Ltd. (Suwon, Gyeonggi-do). The dry powder in the test substance was used by dissolving in sterile purified water.

Example 1 Evaluation of Hangover Relief Efficacy

SPF [240 mg / kg (1.6 mL / kg at 15%) or 750 mg / kg (5 mL / kg at 15%), anthocyanin (750 mg / kg) or SPF-liquid in rats for evaluation of hangover prevention Component (750 mg / kg) was administered orally. After 30 minutes, oral administration of 3 mL / kg (20 mL to 15 mL / kg) of ethanol was performed. After 1, 3 and 5 hours, blood levels of ethanol and acetaldehyde were measured by gas chromatography (Chen, 1995).

In order to evaluate the detoxification effect, the test substance was administered at 1 hour, the maximum blood concentration reaching time after ethanol administration, and the blood concentrations of ethanol and acetaldehyde were measured by gas chromatography after 1, 3, and 5 hours of ethanol administration. Hangover efficacy was evaluated by the degree of absorption and excretion rate in the body. The conditions of gas chromatography for blood ethanol and acetaldehyde analysis are as follows.

Gas Chromatography: Varian CP-3800

Column: Innowax (15 m, 0.25 mm, 0.5 μm)

Oven temperature: 50 ° C., 3 minutes (isothermal)

Pressure: 8.8 psi

Medium gas: He

Injection temperature: 230 ℃

-Detection temperature: 250 ℃

-Head space autosampler (65 ℃ incubation)

Example 2 Evaluation of Inhibitory Effect of Alcoholic Gastric Ulcer

Rats were first orally administered SPF (30, 100 or 300 mg / kg), anthocyanin (300 mg / kg) or SPF-liquid component (300 mg / kg) to evaluate gastric ulcer preventive efficacy. After 30 minutes, the ethanol of the stock solution was orally administered at 3 mL / kg, and animals were sacrificed by anesthesia with diethyl ether 1 hour after ethanol administration. After the stomach was removed, 10 mL of formalin (1%) was injected, inflated with a solution, and cut into species along a Taiwan valley. The stomach was fixed on the cork board and fixed with a pin, and then the length of the gastric ulcer lesion was measured. In the case of spot bleeding, the gastric ulcer index (mm ulcer lesion) was calculated by calculating 5 to 1 mm (Qui et al., 2004).

After evaluating the efficacy of the SPF-liquid component and the purified anthocyanin, the effect of improving gastric ulcer on the SPF fraction (water, butanol and methanol fraction) of 100 mg / kg was evaluated in order to determine the fraction containing the active ingredient. In this procedure, 0.5 mL / kg of 1% Evan's blue was administered intravenously immediately after oral administration of ethanol. After determining the gastric ulcer index, Evan's blue was extracted for 24 hours by soaking gastric tissue in 5 mL of formamide (Filaretova et al., 2002). Absorbance was measured at 620 nm with a spectrophotometer to quantify Evan's blue leaking from the standard calibration curve.

1 is purple sweet potato yeast fermentation filtrate (SPF: ■, 240 mg / kg; ◆, 750 mg / kg), liquid component fraction (△, 750 mg / kg), purified anthocyanin (○, 750 mg / kg), 30 minutes after HDE (▽, 750 mg / kg) administration, ethanol (●, 3 mL / kg) was a graph showing the change in alcohol blood concentration in rats.

Figure 2 shows acetaldehyde in rats when ethanol (●, 3 mL / kg) was administered 30 minutes after the purple sweet potato yeast fermentation filtrate (SPF: ■, 240 mg / kg; ◆, 750 mg / kg) It is a graph showing the change in concentration.

Figure 3 shows purple sweet potato yeast fermentation filtrate (SPF: ■, 240 mg / kg; ◆, 750 mg / kg), liquid component fraction (△, 750 mg / 1 hour after ethanol (●, 3 mL / kg) administration kg), tablet anthocyanin (○, 750 mg / kg), HDE (▽, 750 mg / kg) administration of the blood plasma concentration in the rat is a graph showing the change.

Figure 4 shows acetaldehyde in rats when purple sweet potato yeast fermentation filtrate (SPF: ■, 240 mg / kg; ◆, 750 mg / kg) was administered 1 hour after ethanol (●, 3 mL / kg) administration. It is a graph showing the change in concentration.

FIG. 5 is a diagram showing gastrointestinal bleeding treated with ethanol (3 mL / kg) alone, and FIG. 5 is a photograph showing gastrointestinal tissue when ethanol and purple sweet potato yeast fermentation filtrate (300 mg / kg) are administered together.

Figure 6 is a photograph showing the state of the gastrointestinal ulcer induced by ethanol (3 mL / kg) when the purple sweet potato yeast fermentation filtrate (SPF) and SPF liquid component fractions are administered together.

Fig. 6 is the ethanol alone administration, Fig. 6 is the water fraction 100 mg / kg of SPF liquid component fraction, butanol fraction 100 mg / kg in Fig. 6 rats of the methanol fraction 100 mg / kg in Fig. 6 It shows an ulcer state.

Claims (4)

Fermented purple sweet potato with Saccharomyces yeast strain at 60-70 ° C. for 42-54 hours and filtered sweet potato yeast fermented filtrate (SPF), or sweet potato fermented filtrate extract liquid from which anthocyanin was separated and removed from the fermented filtrate Method of using fraction as a hangover detoxification substance for lowering ethanol and aldehyde blood levels Fermented purple sweet potato with Saccharomyces yeast strain at 60-70 ° C. for 42-54 hours and filtered sweet potato yeast fermented filtrate (SPF), or sweet potato fermented filtrate extract liquid from which anthocyanin was separated and removed from the fermented filtrate Method of using fraction as an alcoholic gastric ulcer inhibitor According to claim 2, Butanol fraction material obtained by separating the liquid component fraction of sweet potato fermentation filtrate extracted from the fermentation filtrate separated by an anthocyanin into a water fraction layer, methanol fraction layer and butanol fraction layer as an alcoholic gastric ulcer inhibitor How to use The method according to claim 1 or 2, wherein the hangover detoxification or alcoholic gastric ulcer suppressant of the sweet potato yeast fermentation filtrate is a substance other than anthocyanin.

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