KR20100042192A - Method for preparation of matured dried persimmon using enzyme - Google Patents

Method for preparation of matured dried persimmon using enzyme Download PDF

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KR20100042192A
KR20100042192A KR1020080101362A KR20080101362A KR20100042192A KR 20100042192 A KR20100042192 A KR 20100042192A KR 1020080101362 A KR1020080101362 A KR 1020080101362A KR 20080101362 A KR20080101362 A KR 20080101362A KR 20100042192 A KR20100042192 A KR 20100042192A
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enzyme
persimmon
fermentation
dried
dried persimmon
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KR101093210B1 (en
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윤동욱
박성진
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주식회사 하눌
김노수
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/03Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/10Drying, dehydrating

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
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Abstract

PURPOSE: A method for manufacturing fermented dried persimmon using natural enzyme is provided to improve aesthetic properties and palatability, and to reduce an aging period of the persimmon while reducing failure rates of products. CONSTITUTION: A method for manufacturing fermented dried persimmon includes the following steps: making dried persimmon by peeling off persimmon; applying fermenting enzyme on the dried persimmon; fermenting the dried persimmon for a constant period; and aging the fermented persimmon for a specified period. The fermenting enzyme is natural enzyme gained by aging and fermenting natural fruits, cereals and seaweed for 4 years. A fermentation period of the persimmon is 26 ~ 30 days and an aged period of the persimmon is 2~6 days.

Description

효소를 이용한 발효곶감의 제조방법{Method for preparation of matured dried persimmon using enzyme}Method for preparation of fermented dried persimmon using enzyme {Method for preparation of matured dried persimmon using enzyme}

본 발명은 효소를 이용한 발효곶감의 제조방법에 관한 것으로, 더욱 상세하게는 수확한 생감의 껍질을 깎아 탈피된 생감을 건조하여 된 곶감의 표면에 발효효소를 도포하여 일정기간 발효시킨 후, 숙성시켜 되는 효소를 이용한 발효곶감의 제조방법에 관한 것이다.The present invention relates to a method for producing fermented dried persimmon using an enzyme, and more particularly, by fermenting a fermented enzyme to a surface of dried persimmon dried by peeling the peeled raw persimmon and fermenting for a certain period of time, then aging. It relates to a method for producing fermented dried persimmon using an enzyme.

일반적으로 곶감은 명절이나 제사 때 쓰는 과일의 하나로, 장기간 저장할 수 있는 것으로, 건시(乾)라고도 한다. In general, dried persimmon is one of the fruits used during holidays and sacrifices, and can be stored for a long time.

곶감 만드는 법은 ≪규합총서≫에 의하면, 8월에 잘 익은 단단한 수시(水; 물감)를 택하여 껍질을 벗기고 꼭지를 떼어 큰 목판에 펴놓아 비를 맞지 않도록 말린다. 위가 검어지고 물기가 없어지면 뒤집어놓고, 마르면 또 뒤집어 말린다고 하였다.The method of making dried persimmons, according to the book, is to pick ripe hard water in August and peel off the peels, remove the faucet and spread it on a large wooden board to avoid rain. When the stomach is black and dry, it turns over, and when it dries, it turns over again and dry.

다 말라서 납작해지면 모양을 잘 만들어 물기 없는 큰 항아리에 켜켜로 넣는다. 감껍질을 같이 말려 켜켜로 격지를 두고 위를 덮는다. 그런 다음에 좋은 짚으 로 덮어 봉하여 두었다가 시설(雪 : 곶감거죽에 돋은 흰가루)이 앉은 뒤에 꺼내면 맛이 더욱 좋다고 알려져 있다. When it is dry and flat, make a good shape and put it in a large, dry jar. Dry the persimmons together and leave them on top to cover them. It is then known to taste better if it is covered with good straw and then taken out after installation (雪: white powder on dried persimmon).

대규모의 곶감 제조에 있어서는 유황훈증(硫黃燻蒸 : 유황 연기를 쐬어서 찜.)을 하여 질을 향상시키고 있다. 이것은 껍질을 벗긴 감을 유황으로 30~40분간 훈증하여 햇볕을 잘 쬔다. 그리고 통풍이 잘 되는 건조장으로 옮겨서 30~38℃에서 4~5일간 건조시키는 방법이다.In the production of large scale dried persimmon sulfur fumigation (硫黃 燻蒸: steamed by steaming steam.) To improve the quality. This fumigation peeled persimmon with sulfur for 30 to 40 minutes to sunshine well. It is then transferred to a well-ventilated drying room and dried at 30-38 ℃ for 4-5 days.

감이 황갈색으로 되고 외피가 굳어지기 시작하면 과육을 손으로 문질러 연하게 하고 씨를 빼낸 다음에 구멍을 합쳐서 건시모양을 만든다. 3주일이 지나서 곶감의 무게가 생감 무게의 75% 정도 되고, 손가락으로 누르면 과육이 들어갔다가 곧 원상으로 돌아올 정도가 되면 거두어들인다.When the persimmons turn yellowish brown and the outer shell starts to harden, the flesh is rubbed with your hands to soften, the seeds are removed, and the holes are combined to form a dry shape. After three weeks, the weight of dried persimmon is about 75% of the weight of raw persimmon, and when you press it with your finger, the pulp enters and is returned to its original shape.

상자에 잘 말린 짚을 켜켜로 깔고 곶감을 담아 시원한 곳에 두어 곶감의 표면에 흰 당분가루가 덮이도록 한다. 감의 껍질을 벗길 때는 쇠로 만든 칼을 사용하면 감에 함유되어 있는 탄닌과 반응하여 빛깔이 변하므로 스테인레스 스틸로 만든 칼을 사용하는 것이 좋다.Put dried straw in a box and put dried persimmon and put it in a cool place so that the surface of dried persimmon is covered with white sugar powder. When peeling persimmons, a knife made of iron reacts with the tannins in the persimmon to change its color, so a stainless steel knife is recommended.

감은 무게에 비하여 건조면이 좁으므로 갑자기 말리는 것을 피하는 것이 좋다. 너무 건조시키면 과육이 굳어지고 건조가 부족하면 곰팡이가 생기기 쉬우므로 매우 조심하여야 한다. 곶감의 조성분은 수분 32%, 단백질 6.3%, 지방 0.44%, 탄수화물 44.8%, 섬유질 15%, 회분 1.99%로 되어 있다.Persimmons are narrower than dry weight, so avoid sudden drying. If you dry too much, the pulp hardens, and if the drying is insufficient, it is easy to form mold, so be very careful. The composition of dried persimmon is 32% water, 6.3% protein, 0.44% fat, 44.8% carbohydrate, 15% fiber, and 1.99% ash.

곶감은 그대로 먹기도 하고, 곶감에 호두를 싸서 곶감쌈을 만들기도 한다. 실백을 박아 숙실과(한과를 말함)이나 생실과(생과일)에 곁들여 놓기도 할 뿐 아니 라, 수정과의 주원료이기도 하다. Dried persimmons are eaten as they are, or dried persimmons are wrapped in walnuts. It is not only added to the fruit and fruit (same) or raw fruit (fresh fruit), but also the main ingredient of the crystal fruit.

근래에 들어서는 곶감의 숙성효과와 저장성, 기능성을 향상시키기 위한 다양한 곶감의 제조방법이 실시되고 있다. Recently, various methods of producing persimmons have been carried out to improve the ripening effect, shelf life, and functionality of dried persimmons.

그 예로서, 등록특허 제10-0762471호에는 녹차잎에 함유된 카테킨류의 성분이 파괴됨이 없이 주정에 추출되게 하므로써 주정녹차추출액을 얻고, 상기 추출액에 탈피된 생감을 침지시키거나 추출액을 분무하는 등의 방법으로 곶감을 제조하는 녹차성분이 함유된 곶감의 제조방법이 개시되어 있고, 또한, 등록특허 제10-0621943호에는 미생물에 대한 살균력과 건강개선 효능을 지닌 목초액과 계피향료를 곶감의 제조공정 중에 사용하여 저장성과 기호성을 향상시킨 곶감의 제조방법이 개시되어 있다. As an example, Patent No. 10-0762471 discloses brewing green tea extract by allowing extracts of catechins contained in green tea leaves to be brewed without being destroyed, and immersing the peeled raw fruit in the extract or spraying the extract. There is disclosed a method for producing dried persimmon containing green tea ingredients for producing dried persimmon, etc., In addition, Patent No. 10-0621943 has been prepared for the production of dried persimmon and cinnamon flavored wood vinegar having a bactericidal power and improved health Disclosed is a method for producing dried persimmons, which is used during the process to improve storage and palatability.

상기의 종래기술처럼 다양한 곶감의 제조방법이 연구되어지고 있으며, 본 발명자들 또한 곶감의 새로운 제조방법을 제공하고자 한다.Various methods of manufacturing persimmon have been studied as in the prior art, and the present inventors also intend to provide a new method of manufacturing persimmon.

본 발명의 목적은 종래의 곶감에 발효효소를 도포하여 발효 및 숙성시킴으로서 종래의 곶감제조방법에 비해 불량률을 낮추고, 숙성능을 향상시켜 숙성기간을 단축시키고, 위생적이며, 심미성 및 기호성이 향상된 발효곶감의 제조방법을 제공하는 것이다. The purpose of the present invention is to apply the fermentation enzymes to the conventional persimmon fermentation and aging by lowering the defective rate compared to the conventional method for producing persimmon, improving the aging performance to shorten the maturation period, hygienic, improved aesthetics and palatability It is to provide a manufacturing method.

또한, 본 발명의 다른 목적은 숙성기간의 단축으로 생산성, 소비성 등이 향상된 발효곶감의 제조방법을 제공하는 것이다.In addition, another object of the present invention is to provide a method for producing a fermented dried persimmon having improved productivity, consumption, etc., by shortening the aging period.

또한, 본 발명의 다른 목적은 일차가공된 곶감을 발효시키는 2차 가공과정을 거치면서 숙최해소능과 같은 새로운 기능성이 향상된 발표곶감의 제조방법을 제공하는 것이다.In addition, another object of the present invention is to provide a method for producing a dried persimmon improved new functionality such as the scouring capacity while going through the secondary processing to ferment the dried persimmon.

상기한 목적을 달성하기 위하여, 본 발명은 수확한 생감의 껍질을 깎아 탈피된 생감을 건조하여 얻어진 곶감의 표면에 발효효소를 도포하여 일정기간 발효시킨 후, 숙성시켜 되는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법을 제공한다.In order to achieve the above object, the present invention by applying a fermentation enzyme on the surface of the dried persimmon obtained by cutting the peeled raw persimmon peeled and dried fermented for a period of time, using the enzyme characterized in that it is aged It provides a method of producing fermented dried persimmon.

또한, 본 발명은 하기 단계를 포함하는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법을 제공한다. In addition, the present invention provides a method for producing a fermented dried persimmon using an enzyme comprising the following steps.

수확한 생감의 껍질을 깎아 탈피하고 건조하여 곶감을 얻는 공정;Peeling and drying the harvested raw persimmons to obtain dried persimmons;

상기 곶감에 발효효소를 도포하는 공정;Applying a fermentation enzyme to the dried persimmon;

상기 발효효소가 도포된 곶감을 상온에서 일정기간 발효시키는 공정; 및 Fermenting the dried persimmons coated with the fermentation enzyme at room temperature for a predetermined time; And

상기 발효된 곶감을 일정기간 숙성시키는 공정.A step of ripening the fermented dried persimmon for a certain period.

또한, 본 발명은 상기 발효효소는 천연과실, 곡류 및 해조류를 4년간 숙성 발효시켜 얻어진 천연효소인 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법을 제공한다.In addition, the present invention provides a method for producing a fermented dried persimmon using an enzyme, characterized in that the fermented enzyme is a natural enzyme obtained by fermenting natural fruit, cereals and seaweeds for 4 years.

또한, 본 발명은 상기 발효효소 도포 후 발효기간은 26 ~ 30일, 숙성기간은 2~6일간이며, 상기 발효효소가 도포된 곶감을 발효 및 숙성시 발효용기에 넣어 일정간격지도록 적층시켜 발효 및 숙성시키는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법을 제공한다.In addition, the present invention is a fermentation period of 26 to 30 days after the application of the fermentation enzyme, the fermentation period is 2 to 6 days, when the fermented fermented enzyme is applied to the fermentation container and put into a fermentation container at a certain interval during fermentation and fermentation and It provides a method for producing a fermented dried persimmon using an enzyme, characterized in that aging.

또한, 본 발명은 상기 발효효소가 도포된 곶감을 숙성시 발효용기에 넣어 일정간격지도록 적층시켜 숙성시키는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법을 제공한다.In addition, the present invention provides a method for producing a fermented dried persimmon using the enzyme, characterized in that the fermentation enzyme-coated dried persimmon is put into a fermentation vessel and laminated so as to be spaced apart at a certain interval.

또한, 본 발명은 상기 발효용기는 내부에 적층판을 순차적으로 적층시키는 구조를 가지며, 스틸인 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법을 제공한다.In addition, the present invention provides a method for producing a fermented dried persimmon using an enzyme, characterized in that the fermentation vessel has a structure to sequentially laminate the laminated plate therein, steel.

본 발명에 따르는 효소를 이용한 발효곶감의 제조방법은 천연과실, 곡류 및 해조류를 4년간 숙성시켜 얻어진 천연효소를 사용함으로써 곶감의 숙성능을 향상시키고, 곶감에 천연과실, 곡류 및 해조류 등에 함유되어 있는 각종 영양소를 곶감에 부가시켜 곶감의 영양성을 향상시킴과 동시에 과육을 부드럽게 하여 식용성, 기호성 및 심미성을 향상시키는 효과를 가진다.Fermented dried persimmon production method using the enzyme according to the present invention improves the ripening performance of dried persimmon by using natural enzymes obtained by ripening natural fruits, cereals and seaweeds for 4 years, and the dried persimmons contained in natural fruits, cereals and seaweeds Various nutrients are added to dried persimmons to improve the nutrition of dried persimmons and at the same time to soften the pulp and have the effect of improving edibility, palatability and aesthetics.

또한, 본 발명은 곶감을 발효시켜 숙성시킴으로써 생산성을 향상시키고, 또한, 외부 환경에 영향을 받지 않도록 발효용기에 넣어 숙성시킬 수가 있어 위생성도 향상시키는 효과를 가진다. In addition, the present invention improves productivity by fermenting dried persimmon and fermenting it so that it can be put into fermentation vessels so as not to be affected by the external environment, thereby improving hygiene.

이하, 본 발명을 보다 상세히 설명한다. Hereinafter, the present invention will be described in more detail.

본 발명은 발효곶감의 제조방법에 관한 것으로서, 본 발명에서는 수확한 생감의 껍질을 깎아 탈피된 생감을 건조하여 곶감을 얻고, 상기 곶감의 표면에 발효효소를 도포하여 발효, 숙성시킴으로서, 불량률을 낮추고, 곶감의 기호성 및 영양성, 그리고 생산성을 향상시키는 것에 발명의 특징이 있다. The present invention relates to a method for producing fermented dried persimmon, in the present invention by cutting the peeled raw persimmon peeled dried persimmon to obtain dried persimmon, by applying fermentation enzymes on the surface of the dried persimmon fermentation, aging, lowering the defective rate The characteristics of the invention are to improve the palatability and nutrition of dried persimmons and productivity.

본 발명의 일예로 하기와 같은 공정으로 곶감을 제조한다. As an example of the present invention, dried persimmon is prepared by the following process.

즉, 수확한 생감의 껍질을 깎아 탈피하고 건조하여 곶감을 얻는 공정;That is, a step of peeling and drying the harvested persimmon peel to obtain dried persimmon;

상기 곶감에 발효효소를 도포하는 공정;Applying a fermentation enzyme to the dried persimmon;

상기 발효효소가 도포된 곶감을 상온에서 일정기간 발효시키는 공정; 및 Fermenting the dried persimmons coated with the fermentation enzyme at room temperature for a predetermined time; And

상기 발효된 곶감을 일정기간 숙성시키는 공정.A step of ripening the fermented dried persimmon for a certain period.

상기 공정에 있어서, 사용되는 상기 발효효소는 일반적인 효소를 사용할 수도 있으나, 천연과실, 곡류 및 해조류를 숙성 발효시켜 얻어진 천연효소를 사용하는 것이 바람직하며, 더 좋게는 상기 천연과실, 곡류 및 해조류를 4년간 숙성 발효시켜 얻어진 천연효소를 사용하는 것이 바람직하다. In the above process, the fermentation enzyme used may be a general enzyme, but it is preferable to use a natural enzyme obtained by aging and fermenting natural fruit, cereals and algae, and more preferably the natural fruit, grains and seaweed 4 It is preferable to use natural enzymes obtained by fermentation for a year.

이때, 상기 천연효소는 정제수 등에 효소액을 일정량 혼합한 후, 혼합제조된 효소액을 분무기 등으로 도포하거나, 혹은 일정 부피를 가지는 용기에 효소액을 담고, 상기 효소액에 곶감을 침지시켜 표면에 도포되도록 한다.At this time, the natural enzyme is mixed with a predetermined amount of the enzyme liquid, such as purified water, the mixed enzyme solution is applied by a sprayer or the like, or the enzyme solution is contained in a container having a predetermined volume, so as to immerse the dried persimmon in the enzyme solution to be applied to the surface.

또한, 상기 발효공정에서 발효기간은 26 ~ 30일간 발효시키는 것이 좋으며, 더 바람직하게는 27 ~ 29일간 발효시켜 숙성시키는 것이 더 좋다.In addition, the fermentation period in the fermentation process is good to ferment for 26 to 30 days, more preferably 27 to 29 days of fermentation is more preferable.

또한, 상기 숙성공정에서 숙성기간은 2~6일간 숙성시키는 것이 바람직하며, 더 바람직하게는 2~4일간 숙성시키는 것이 좋다.In addition, the aging period in the aging step is preferably aged for 2 to 6 days, more preferably for 2 to 4 days.

본 발명에 의하면, 상기 발효효소가 도포된 곶감을 발효, 숙성시 통상의 곶감을 제조하는 것과 같은 방식으로 발효, 숙성시켜도 되나, 더 좋게는 위생적인 생산을 위하여 별도의 발효용기를 준비하고, 상기 발효용기에 넣어 일정간격지도록 적층시켜 발효, 숙성시키는 것이 바람직하다. 이때, 상기 발효용기는 통상 사용되는 건조장을 사용하기 보다는 일정 온도를 유지하는 데 유리한 스틸로 별도 제작된 발효용기를 사용하는 것이 바람직하다.According to the present invention, the fermented fermented persimmon coated with fermentation, may be fermented and matured in the same manner as the conventional dried persimmons are prepared during fermentation, more preferably, prepare a separate fermentation vessel for hygienic production, The fermentation vessel is preferably laminated so as to be spaced at a predetermined interval to ferment and ripen. In this case, it is preferable that the fermentation vessel uses a fermentation vessel separately made of steel, which is advantageous for maintaining a constant temperature, rather than using a dry field that is usually used.

여기서, 상기 발효용기는 발효효소가 도포된 곶감을 순차적으로 적층가능하도록 일정면적을 가지는 적층판이 적층되는 구조로 되는 것을 사용하는 것이 좋다. Here, the fermentation vessel is preferably used that is a structure in which a laminated plate having a predetermined area is laminated so that the dried persimmon coated with fermentation enzyme can be sequentially stacked.

상기 적층판에는 상기 발효효소가 도포된 곶감을 일정간격지도록 나열하고, 상기 곶감이 나열된 적층판을 공기의 자연순환이 이루어지도록 소정 높이를 가지도록 적층하는 것이 바람직하다. It is preferable to arrange the dried persimmons coated with the fermentation enzyme on the laminated plates at a predetermined interval, and to laminate the laminated plates on which the dried persimmons have a predetermined height so that natural circulation of air is achieved.

또한, 상기 발효용기는 밀폐식보다는 자연 통풍이 되도록 제조된 것을 사용하는 것이 바람직하다. In addition, the fermentation vessel is preferably used to be a natural ventilation rather than hermetic.

이하, 본 발명을 바람직한 실시예를 들어 설명하기로 한다. 이하, 실시예로 본 발명을 한정하는 것은 아니다. Hereinafter, the present invention will be described with reference to preferred embodiments. Hereinafter, the present invention is not limited to the Examples.

[실시예]EXAMPLE

1. 재료 및 방법1. Materials and Methods

① 재료: 일반적인 상법에 따라 제조된 곶감을 이용① Material: using dried persimmon manufactured according to the common commercial method

② 곶감의 준비② Preparation of dried persimmon

일반적인 상법에 따라 제조하여된 곶감을 준비하여 사용하였다.Dried persimmons prepared according to the usual commercial methods were prepared and used.

③ 천연효소 : 사과, 쌀 및 멸치를 4년간 발효시킨 효소액을 사용하였다.③ natural enzyme: apple, rice and anchovy was used for 4 years fermented enzyme solution.

④ 천연효소액 도포시기 및 방법: 상기 천연효소액 0.3ℓ를 혼합한 천연효소혼합액을 제조하고, 곶감을 상기 혼합액에 침지, 교반하여 표면에 골고루 도포하였다.④ Application time and method of natural enzyme solution: A natural enzyme mixture was prepared in which 0.3 L of the natural enzyme solution was mixed, and dried persimmon was immersed in the mixed solution, followed by stirring.

⑤ 발효곶감의 제조 : 천연효소가 도포된 곶감을 28일간 발효시킨 후, 2일간 숙성시켜 제조하였다. ⑤ Preparation of fermented dried persimmon: Fermented dried persimmon coated with natural enzyme for 28 days, and then matured for 2 days.

⑥ 기능성 검사 : 하기의 방법으로 제조된 발효곶감의 기능성을 시험하였다. ⑥ functional test: The functionality of fermented dried persimmon prepared by the following method was tested.

- 시험용 마우스에 알콜섭취 후 30, 60, 120분후 체혈한 후, 혈중 알콜농도, 아세트알데하이드 농도, AST(GOT), ALT(GPT) 농도 측정-After 30, 60, 120 minutes after the alcohol intake in the test mice, blood alcohol concentration, acetaldehyde concentration, AST (GOT), ALT (GPT) concentration measurement

<혈중알콜농도>Blood Alcohol Concentration

- 검사방법 : 알코올 탈수소효소에 의한 효소법-Test method: Enzyme method by alcohol dehydrogenase

- 검사원리 : 에탄올의 효소검사는 다음 반응식과 같이 NAD와 ADH(알코올 탈수소효소)를 이용한다. Principle of Test: Enzyme test of ethanol uses NAD and ADH (alcohol dehydrogenase) as shown in the following equation.

Figure 112008071875134-PAT00001
Figure 112008071875134-PAT00001

- NADH의 형성은 378nm에서 흡광도 증가로 정량할 수 있다. 1,3-디아미노-2-하이드록시프로판이 알데하이드를 포획하는 시약이며, 효과적인 완층액으로 작용한다. 이러한 시약계는 알코올 탈수소효소를 이용하는 에탄올 정량법으로 다른 검사방법에 비해 안정성이 있다.Formation of NADH can be quantified by increased absorbance at 378 nm. 1,3-diamino-2-hydroxypropane is a reagent that captures aldehydes and acts as an effective slow effluent. This reagent system is ethanol quantitative determination using alcohol dehydrogenase is more stable than other test methods.

- 참고치 : 에탄올을 섭취하였을 경우를 제외하고, 정상상태에서는 혈액 내에 에탄올은 발견되지 않는다. -Reference value: No ethanol is found in blood under normal condition except when ethanol is ingested.

- 사용시약 : Alcohol (제조사 : Roche-BM / 제조국 : Switzerland )-Reagent: Alcohol (Manufacturer: Roche-BM / Country of origin: Switzerland)

- 측정기기 : Cobas-integra (제조사 : Roche-BM / 제조국 : Switzerland)-Measuring equipment: Cobas-integra (Manufacturer: Roche-BM / Country of manufacture: Switzerland)

- 측정결과는 표1 및 도1에 개시됨The measurement results are shown in Table 1 and FIG.

<아세트알데하이드 농도>Acetaldehyde concentration

- 검사방법 : UV method -Inspection method: UV method

- 검사원리 : -Inspection Principle:

Figure 112008071875134-PAT00002
Figure 112008071875134-PAT00002

Acetaldehyde는 NAD와 물, Al-DH 효소에 의해 NADH와 H로 가수분해되고, Acetic acid로 산화된다.  Acetaldehyde is hydrolyzed to NADH and H by NAD, water and Al-DH enzymes and oxidized to Acetic acid.

검체중에 NADH화된 acetaldehyde 양을 334, 340 또는 365 nm 에서 측정한다.The amount of NADHylated acetaldehyde in the sample is measured at 334, 340 or 365 nm.

- 사용시약 : Acetaldehyde Cat.no.668613 (제조사 :BOEHRINGER MANNHEIM, 제조국 :Germany)-Reagent: Acetaldehyde Cat.no.668613 (Manufacturer: BOEHRINGER MANNHEIM, Country of origin: Germany)

- 측정기기 : UV spectrophotometer U-3010 (제조사 : Hitachi, 제조국 : Japan)-Measuring instrument: UV spectrophotometer U-3010 (manufacturer: Hitachi, country of origin: Japan)

- 측정결과는 표2 및 도2에 개시됨The measurement results are shown in Table 2 and FIG.

<AST 농도><AST concentration>

- 검사방법 : IFCC UV method(without pyridoxal phosphate without sample blank)-Test method: IFCC UV method (without pyridoxal phosphate without sample blank)

- 검사원리 : -Inspection Principle:

Figure 112008071875134-PAT00003
Figure 112008071875134-PAT00003

NADH의 흡광도 감소율에 의해 GOT활성치를 구한다. The GOT activity value is obtained from the absorbance reduction rate of NADH.

- 참 고 치 : < 38 IU/L -Note: <38 IU / L

- 사용시약 : AST (제조사 : Simens / 제조국 : U.S.A)-Reagent: AST (Manufacturer: Simens / Country of manufacture: U.S.A)

- 측정기기 : ADVIA 1650 (제조사 : Simens / 제조국 : U.S.A)-Measuring equipment: ADVIA 1650 (manufacturer: Simens / country of manufacture: U.S.A)

- 측정결과는 표3 및 도3에 개시됨The measurement results are shown in Table 3 and FIG.

<ALT 농도><ALT concentration>

- 검사방법 : IFCC UV method(without pyridoxal phosphate without sample blank)-Test method: IFCC UV method (without pyridoxal phosphate without sample blank)

- 검사원리 :-Inspection Principle:

Figure 112008071875134-PAT00004
Figure 112008071875134-PAT00004

NADH의 흡광도 감소율에 의해 GPT활성치를 구한다.The GPT activity value is obtained from the absorbance reduction rate of NADH.

- 참고치 : < 43 IU/L Reference value: <43 IU / L

- 사용시약 : ALT (제조사 : Simens / 제조국 : U.S.A )-Reagent: ALT (Manufacturer: Simens / Country: U.S.A)

- 측정기기 : ADVIA 1650 (제조사 : Simens / 제조국 : U.S.A )-Measuring equipment: ADVIA 1650 (manufacturer: Simens / country of manufacture: U.S.A)

- 측정결과는 표4 및 도4에 개시됨.The measurement results are shown in Table 4 and FIG.

⑦ 각 시험군 준비 및 시험: ⑦ Preparation and test of each test group:

- 각 시험군 그룹당 실험용 마우스 개체수는 6마리 -6 experimental mouse populations per group

- 발효곶감 및 시판품(자연건조 곶감)의 섭취량은 100g, 알콜섭취량: 성인기준 소주 1병-Intake of fermented dried persimmon and commercial products (natural dried dried persimmon) is 100g, alcohol intake: 1 bottle of adult shochu

- 혈액체취는 30분, 60분, 120분 후 각각의 개체당 3회 체취, 상기 기능성 검사 방법에 따라 각각의 혈액내의 변화를 검사함.Blood odor is three times for each individual after 30 minutes, 60 minutes, and 120 minutes, and the change in blood is examined according to the functional test method.

⑧ 관능검사 : 훈련된 관능요원 10명이 곶감의 표면 형태, 맛, 향, 촉감 및 기호도에 대하여 평가하였다. 평가는 상, 중, 하로 구분하여 실시하였다.(표 5)⑧ Sensory Evaluation: Ten trained sensory personnel evaluated the surface morphology, taste, aroma, touch and palatability of dried persimmons. Evaluation was performed by dividing into upper, middle and lower (Table 5).

2. 결과 및 요약2. Results and Summary

ConCon 발효곶감Fermented dried persimmon 시판품Commercial item 30분30 minutes 48 48 48 48 55 55 60분60 minutes 27 27 21 21 38 38 120분120 minutes 5 5 2 2 2 2

<결과><Result>

상기 표 1에서 보는 바와 같이 발효곶감을 섭취시 시간 경과에 따라 체내의 알콜 분해가 훨씬 빠르게 이루어짐을 알 수 있다.As shown in Table 1, it can be seen that alcohol degradation in the body is made much faster over time when the fermented dried persimmon is ingested.

ConCon 발효곶감Fermented dried persimmon 시판품Commercial item 30분30 minutes 0.151 0.151 0.201 0.201 0.175 0.175 60분60 minutes 0.118 0.118 0.139 0.139 0.168 0.168 120분120 minutes 0.063 0.063 0.042 0.042 0.043 0.043

<결과><Result>

상기 표 2에서 보는 바와 같이, 본 발명의 발효곶감을 섭취한 개체군이 시간이 경과함에 따라 아세트알데하이드의 농도가 빠르게 줄어드는 것을 알 수 있다.As shown in Table 2, it can be seen that the concentration of acetaldehyde is rapidly reduced over time the population ingested fermented dried persimmon of the present invention.

ConCon 발효곶감Fermented dried persimmon 시판품Commercial item 30분30 minutes 119 119 116 116 111 111 60분60 minutes 158 158 132 132 127 127 120분120 minutes 141 141 115 115 122 122

ConCon 발효곶감Fermented dried persimmon 시판품Commercial item 30분30 minutes 51 51 54 54 48 48 60분60 minutes 61 61 59 59 55 55 120분120 minutes 57 57 47 47 52 52

<결과><Result>

상기 표 3 및 4에서 보여 지는 바와 같이, 알콜 분해에 따른 간손상 지표인 혈중 AST 및 ALT의 변화를 볼 때 발효곶감의 섭취로 혈중 AST 및 ALT의 증가를 감소시켜 알콜 분해에 대한 간 기능 손상을 줄여주는 것으로 확인할 수 있다. As shown in Tables 3 and 4 above, the changes in blood AST and ALT, which are indicators of liver damage due to alcohol degradation, decreased liver function impairment for alcohol degradation by reducing the increase of blood AST and ALT by ingestion of fermented persimmon. It can be confirmed by reducing.

종목 천연효소도포Natural enzyme application 표면형태Surface shape flavor incense 촉감touch 기호도Symbol Prize medium Ha Prize medium Ha Prize medium Ha Prize medium Ha Prize medium Ha 무처리군No treatment group 44 55 1One 66 44 -- 22 55 33 44 44 22 22 88 -- 처리군Treatment group 77 33 88 22 -- 66 33 1One 55 55 -- 99 1One --

<결과><Result>

상기 표 5에서 보여 지는 바와 같이, 무처리군에 비하여 처리구가 표면형태, 맛, 향, 촉감, 기호도에 있어서 높은 값을 나타내었다. As shown in Table 5, the treatment group showed a higher value in the surface form, taste, aroma, touch, preference than the untreated group.

이상에서 설명한 바와 같이, 본 발명의 효소를 이용한 곶감의 제조방법은 천연과실, 곡류 및 해조류를 4년간 숙성시켜 얻어진 천연효소를 사용함으로써 곶감의 숙성능을 향상시키고, 곶감에 천연과실, 곡류 및 해조류 등에 함유되어 있는 각종 영양소를 곶감에 부가시켜 곶감의 영양성을 향상시킴과 동시에 과육을 부드럽게 하여 식용성을 향상시킴을 알 수 있다. As described above, the method for producing dried persimmon using the enzyme of the present invention improves the ripening performance of dried persimmon by using natural enzymes obtained by aging natural fruits, cereals and seaweeds for 4 years, and the natural fruits, cereals and seaweeds on dried persimmons. Various nutrients contained in the back can be added to the persimmon to improve the nutrition of the persimmon and at the same time to soften the flesh to improve the edible.

또한, 본 발명은 자연건조된 곶감에 비해 기호성이 향상되고, 불량률을 대폭 감소시킴으로써 생산성을 향상시키고, 또한, 외부 환경에 영향을 받지 않도록 발효용기에 넣어 숙성시킬 수가 있어 위생성도 향상시킬 수 있음을 알 수 있다. In addition, the present invention improves the palatability compared to the naturally dried dried persimmon, improves productivity by significantly reducing the defective rate, and also can be put into a fermentation vessel to be aged so as not to be affected by the external environment, thereby improving hygiene. Able to know.

도 1 내지 도 4는 본 발명의 따르는 발효곶감의 기능성 검사 결과를 도시한 그래프이다.1 to 4 is a graph showing the functional test results of fermented dried persimmon according to the present invention.

Claims (5)

수확한 생감의 껍질을 깎아 탈피된 생감을 건조하여 얻어진 곶감의 표면에 발효효소를 도포하여 발효시킨 후, 숙성시켜 되는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법.A method for producing fermented dried persimmon using enzymes, characterized in that the fermented enzyme is applied to the surface of dried persimmon obtained by cutting the peeled raw persimmon and dried, and then fermented. 제 1 항에 있어서, The method of claim 1, 하기 단계를 포함하는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법.Fermented dried persimmon production method using the enzyme, characterized in that it comprises the following steps. 수확한 생감의 껍질을 깎아 탈피하고 건조하여 곶감을 얻는 공정;Peeling and drying the harvested raw persimmons to obtain dried persimmons; 상기 곶감에 발효효소를 도포하는 공정;Applying a fermentation enzyme to the dried persimmon; 상기 발효효소가 도포된 곶감을 상온에서 일정기간 발효시키는 공정; 및 Fermenting the dried persimmons coated with the fermentation enzyme at room temperature for a predetermined time; And 상기 발효된 곶감을 일정기간 숙성시키는 공정.A step of ripening the fermented dried persimmon for a certain period. 제 1 또는 2 항에 있어서, The method of claim 1 or 2, 상기 발효효소는 천연과실, 곡류 및 해조류를 4년간 숙성 발효시켜 얻어진 천연효소인 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법.The fermentation enzyme is a method for producing a fermented dried persimmon using an enzyme, characterized in that the natural enzyme obtained by fermenting natural fruit, cereals and seaweeds for 4 years. 제 1 또는 2 항에 있어서, The method of claim 1 or 2, 상기 발효효소 도포후 발효기간은 26 ~ 30일, 숙성기간은 2~6일간이며, 상기 발효효소가 도포된 곶감을 발효 및 숙성시 발효용기에 넣어 일정간격지도록 적층시켜 발효 및 숙성시키는 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법.Fermentation period after the application of the fermentation enzyme is 26 to 30 days, the fermentation period is 2 to 6 days, when fermentation and fermentation of the dried persimmon coated with fermentation in a fermentation vessel, characterized in that the fermentation and maturation laminated to a certain interval Fermented dried persimmon production method using the enzyme. 제 4 항에 있어서, The method of claim 4, wherein 상기 발효용기는 내부에 적층판을 순차적으로 적층시키는 구조를 가지며, 스틸인 것을 특징으로 하는 효소를 이용한 발효곶감의 제조방법.The fermentation vessel has a structure for sequentially stacking the laminated plate therein, the method for producing fermented dried persimmon using an enzyme, characterized in that the steel.
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KR20220072221A (en) * 2020-11-25 2022-06-02 이종관 Dried Persimmon Powder Manufacturing Method Using Natural Persimmon Fermentation Liquid Species

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* Cited by examiner, † Cited by third party
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KR20220072221A (en) * 2020-11-25 2022-06-02 이종관 Dried Persimmon Powder Manufacturing Method Using Natural Persimmon Fermentation Liquid Species

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