KR20090099374A - Fucoidan-enriched extract of brown-seaweed and its manufacturing method - Google Patents

Abstract

A fucoidan-enriched Undaria pinnatifida extract and a preparing method thereof are provided to increase the pharmacological activity of Undaria pinnatifida for use in health functional foods. A method for preparing a fucoidan-enriched Undaria pinnatifida extract comprises the following step of: extracting fucoidan from brown algae; and adding it to Undaria pinnatifida extract. The brown algae represent sporophyll of Undaria pinnatifida. The Undaria pinnatifida extract is obtained by roughly grinding dried or raw Undaria pinnatifida, adding 7‘±~10‘± water to 1kg dried Undaria pinnatifida or 5‘±~ 10‘± water to 1kg raw Undaria pinnatifida and boiling at 80-120°C for 30-240 minutes. The fucoidan extract is prepared by grinding dried sporophyll, adding 7-15‘± water to 1kg dried sporophyll, adjusting its pH to 2-4, boiling at 80-120°C for 90-240 minutes and separating into a solid and liquid portion. The Undaria pinnatifida extract and fucoidan extract are mixed in constant ratio.

Description

Fucoidan-enriched extract of brown-seaweed and its manufacturing method

The present invention relates to a high-functional pregnant women's food and health food supplemented with fucoidan extracted from Wakame spores in seaweed extract and health food and a manufacturing method thereof. More specifically, the present invention relates to a more complete health food supplemented with fucoidan in seaweed extract by adding fucoidan, a bioactive substance extracted from wakame spores, etc. to the extract of wakame seaweed containing minerals and polysaccharides, and a method of manufacturing the same.

Traditionally, seaweed has been used as a food for postpartum cooking. For mothers, seaweed increases lactation, constricts postpartum uterus, and heals wounds to help postpartum recovery. Basically, seaweed has been established as an essential postpartum care food with functions such as clearing blood, restoring Gi, and promoting metabolism to improve rough skin and improve constipation.

In addition, the seaweed has been used as a functional food and a favorite food for the general public because there are nutrition and functions necessary for humans.

In the early semester of the Chinese Tang Dynasty, there is a record that the Koreans, who saw whales pluck seaweed in order to heal their wounds, gave birth to them to eat seaweed, at least 1300 years ago. We can see that Korean people have used seaweed as an essential food for mothers.

Haebogam (海菜) is said to remove fever and stuffiness, heal the clumps and urinate well.

In Japan, seaweed is known as a postpartum medicine and is used as a postpartum cooked food.

In seaweed, our bodies contain all the minerals our bodies need and their proportions are most similar to those in our bodies (Table 1).

<Table 1> Comparison of minerals contained in RDA (U.S. mineral daily recommended intake) and seaweed

   mineral     RDA mg / day  Mg in 100 g of seaweed   Amount of adult body   Calcium (Ca)       800       1,300     1,000,000   Magnesium (Mg)       350       1,170        19,000   Phosphorus (P)       800         420   Sodium (Na)   1,100-3,500       6,100       100,000   Carium (K)   1,850-5,625       5,500       140,000   Fe        10           7         4,200   Cu        10          34            72   Manganese (Mn)       2 ~ 3           9.5            12   Zinc (Zn)     2.5-5           3.10         2,300   Iodine (I)       1.15           2.4            11   Nickel (Ni)    0.15-0.5           4.6            10   Barium (V)    0.15-5 1/2 to 1/3 of the RDA            18   Chrome Cr       15      Seaweed 10g noodles            16   Seren (Sa)    0.05-0.2       Ingestion            13

Among the minerals, calcium is the most abundant mineral in our body and is a mineral that plays a very important role in life activities. Of the 2% of the body's calcium, about 99% is in bones and teeth, and the remaining 1% is in ions in the blood, cell walls, saliva, and semen.

Small amounts of calcium in the ionic state play an important role in complex mechanisms such as blood coagulation and enzyme activity, as well as regulating all functions of the heartbeat, muscle contraction, hormonal system, brain, eyes and ears.

If you lack calcium, your brain's parathyroid glands secrete hormones that regulate calcium levels, and these regulators drain calcium from bones and teeth. In particular, it is a micronutrient that has more effect on pregnant women.

Iodine is only about 25mg in the body, but it is a very important mineral that acts on thyroid hormones to boost metabolism. If you lack iodine, your metabolism will not go well and your aging will progress quickly.

Sufficient iodine supply is important for metabolic and intelligence development. In addition, it creates elastic skin, shiny hair and affects children's growth and brain development, which shows why seaweed is called IQ foods, growth foods and beauty foods. Iodine is not available at all on land-grown plants, but only in seaweed, such as seaweed.

In addition to seaweed, it has almost all the minerals needed by the human body, such as selenium. In addition to minerals, wakame contains amino acids such as omega-3 polyunsaturated fatty acids DHA and DPA and glutamic acid, cystine, tryptophan and lysine.

Mannitol in seaweed is the best diuretic and is known to be effective in preventing and healing postpartum uterine contractions.

Alginic acid, contained 30% in seaweed, has already been published in thousands of research papers, including detoxification of harmful substances such as heavy metals, cholesterol reduction, hypertension and atherosclerosis prevention, constipation, formal effects, and dietary effects.

Fucoidan is a sulfated polysaccharide whose main component is sulfated fucose (U-focus), and has a structure in which sugars such as galactose, mannose, and xylose, sulfate, and uronic acid are combined. have

Fucoidan is known as a key substance of bioactive substances in brown algae, and the research and published functions show apoptosis, blood purification, antibody immunity, skin regeneration, antiviral, antibacterial, gastrointestinal improvement, It is reported to be effective in preventing and improving all lifestyle diseases caused by constipation, antioxidant activity, and biological balance of physiological effects.

Alginic acid and fucoidan contained in this seaweed are functional substances contained in the slimy slime of seaweed and show good efficacy in modern lifestyle diseases (Table 2).

Table 2 Functionalities of Alginic Acid and Fucoidan

      Alginate       Fucoidan  Absorption of harmful substances such as heavy metals Excretion Cholesterol Reduction Prevention and improvement of hypertension and arteriosclerosis Diabetes improvement effect and prevention Constipation prevention and treatment Prevention stroke Prevention effect Diet effect  Anti-cancer cancer cell extinction effect (Apoptosis-inducing function) Inhibit neovascularization Increase immunity Inhibit blood coagulation Hypertension. Improvement of arteriosclerosis Defense against gastric wall invasion of Vibrio ulcer, repair of mucous membrane of gastric wall H. pylori prevention and treatment

Among them, alginic acid has a large amount of seaweed, so it can be fully functional by simply eating seaweed, but fucoidan, a key substance of bioactive substance, is concentrated in almost discarded seaweed spores (seaweed ear) and is commonly used. Seaweed leaves contain too small amounts, so it is difficult to expect the functionalities of fucoidan from living organisms.

<Table 3> Contents of Fucoidan of Wakame Leaf and Wakame Spores (Bio-based)

   Seaweed Leaf    Seaweed Spores     0.15%       0.8%

The present invention is to solve the problem that the fucoidan in the seaweed we eat contains a trace amount of about 0.15% of the biological standard, and ingesting the seaweed in a general manner does not fully utilize the functions of the fucoidan, which is a key substance of physiological activity.

To this end, the present invention is to increase the pharmacological action of seaweed by extracting the fucoidan from seaweed poscissium or other brown algae algae containing a large amount of fucoidan to the seaweed extract to increase the pharmacological action of the seaweed and the benefit for pregnant women and babies lactating It aims to provide health functional food.

In order to achieve the above object, the seaweed extract reinforced with fucoidan according to an aspect of the present invention is characterized in that it is added to the seaweed extract fucoidan extracted from brown algae, preferably seaweed spores.

In addition, the manufacturing method of seaweed extract reinforced with fucoidan according to another aspect of the present invention; After the salt is removed, the dried seaweed or fresh seaweed is roughly crushed, and the crushed raw material is placed in a filter bag in an appropriate amount, and it is put in a pressure extractor, and the water is put into 7ℓ ~ 10ℓ per 1kg dried seaweed, and 1kg of brown seaweed. After pouring at a rate of 5l ~ 10l, boiled while circulating water in the extractor for 30 minutes to 240 minutes at a temperature of 80 ℃ ~ 120 ℃, after the solid-liquid separation to prepare an extract of seaweed extract; After crushing the dried spores, put the crushed spores in an appropriate amount in a filter bag, put them in a pressure extractor, pour water at a ratio of 7-15 l of water per 1 kg of dried spores, and adjust the pH to 2-4. After the boil while circulating water in the extractor for 90 minutes to 240 minutes at a temperature of 80 ℃ ~ 120 ℃, the solid-liquid separation to obtain an extract to obtain the extract; And a mixing step of mixing the seaweed extract and the fucoidan extract in a predetermined ratio.

Seaweed extract reinforcing fucoidan of the present invention, as well as all of the traditional seaweed-derived active ingredients mothers obtained through seaweed soup, as well as the bioactive substance fucoidan is enhanced because it can be expected to have a better pharmacological effect than seaweed soup.

Moreover, the seaweed extract reinforcing fucoidan of the present invention, unlike the seaweed soup, is portable and easy to take, and does not get tired of continuous taking for a long time, so that it is possible for a pregnant woman's steady intake. This will result in the transfer of active ingredients derived from seaweed to the fetus and the feeding of high quality breast milk to the baby, which will eventually contribute to raising a strong baby.

In addition, the seaweed extract reinforcing fucoidan of the present invention is effective in preventing the adult diseases such as constipation, obesity, high blood pressure and improve the constitution of modern man who is acidified day by day, as a pure natural food without chemical treatment side effects to the human body Since it is not present, it can be widely used as a natural dietary supplement for health and fitness of the whole nation.

Looking at the manufacturing method of the seaweed extract reinforced with fucoidan according to the present invention according to each process as follows.

<Production of Seaweed Extracts>

Crushed and dried salted seaweed or prepared by washing and desalting salted seaweed. The washed seaweed is placed in a pressure extractor at a rate of 5-10 l of water per kg of wakame seaweed and 7-10 l of water per kg of seaweed, and the water in the extractor for 30-240 minutes at a temperature of 80-120 ° C., preferably 160 minutes. After boiling while circulating, the extract is obtained by solid-liquid separation using a filter or a press dehydrator.

Optionally, the seaweed extract may remove pigments and seaweed odors. For example, it is possible to obtain a clear extract by deodorizing using a carbon filter and removing pigments and suspended solids with an ultrafilter.

The extract thus obtained is concentrated to 3 ~ 7brix.

<Production of Fucoidan>

After crushing the dry spores and put the crushed spores in the bag for 40 ~ 100g / ㎡ 3 ~ 5kg each. Put the pressure extractor in the ratio of 7-15ℓ of water per 1kg of dried coriander and adjust hydrochloric acid or organic acid to PH2 ~ 4 and boil it at 80 ~ 120 ℃ for 240 minutes while rotating water for 90 minutes.

When the boiled spores are opened under the pressure extractor, the algae extract in the extractor is automatically separated from the filter bag by the pressure difference between the internal pressure of the pressure extractor and the atmospheric pressure.

The extract thus obtained is obtained by removing alginic acid using known calcium chloride to obtain only fucoidan. The obtained fucoidan is obtained by dialysis and purification using an ultrafilter. At this time, the purity of fucoidan is around 80 ~ 85%.

The fucoidan extract thus extracted is concentrated to 10brix.

<Production of Seaweed Extracts Reinforced Fucoidan>

The seaweed extract and the fucoidan extract are mixed to prepare a seaweed extract reinforced with fucoidan. The mixing ratio of seaweed extract and fucoidan is preferably based on the recommended daily intake of fucoidan. For reference, the daily intake of fucoidan recommended for recovery after childbirth is about 1g to 2g.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to the following examples. However, the following examples are only intended to specifically illustrate the present invention, it is obvious to those skilled in the art that the scope of the present invention is not limited. That is, simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.

Example

1. Preparation of Seaweed Extract

1kg of salted seaweed was washed and desalted, and 0.5kg each of two nonwoven fabric bags of 35 x 50cm was poured in each, and 5ℓ of water was poured into a 30ℓ pressure extractor. The temperature of the extractor was adjusted to a temperature of 120 ° C. and the water in the extractor was rotated up, down, left, and right.

The pressure in the extractor was 1.5 bar (1.5 kgt) when the temperature inside the extractor rose to 120 ° C. After 60 minutes, the fire was turned off after maintaining the temperature of 120 ° C. and the pressure of 1.5 bar. After 20 minutes, the bottom cock of the extractor was opened, and the extract liquid in the extractor was separated by the pressure difference, thereby separating the liquid. The extracted extract was 4 L.

The extracted filtrate was filtered with palm activated carbon and ultrafiltration MW50 only to remove and deodorize suspended solids and pigments, to obtain 2.5 L of seaweed extract, which was concentrated to 5brix to make 990ml of seaweed extract.

2. Extraction of Fucoidan

Roughly crushed 1kg dry seaweed spores coarse into a nonwoven bag of 35 x 50cm in all directions, the bag was placed in a 30l pressure extractor filled with 7l of water and adjusted to PH4.

The temperature of the pressure extractor was adjusted to 120 ° C. and operated in the same manner as the wakame extract to recover 3.8 L of wakame spores extract.

1M calcium chloride solution was prepared in the extract and stirred at a temperature of 30 ° C. for 2 hours.

Precipitated alginic acid was removed to obtain 2 L of crude fucoidan filtrate.

The crude fucoidan was deodorized with palm activated carbon and 500,000 MW of UF (Fall Co., Ltd.), the pigment was removed, and concentrated by dialysis to obtain a purified fucoidan solution of 10brix 250ml.

3. Preparation of Seaweed Extracts Reinforced Fucoidan

Generally, 30g of salted seaweed is a one-time serving of seaweed soup. It is 30 ml in terms of extract. In this extract, 10 ml (1 g) of fucoidan derived from wakame cotyledons can be added for single use. On the basis of this amount, supplemented minerals, alginic acid, and fucoidan in seaweed were prepared to prepare pregnant women's body foods and health functional foods.

Claims (4)

Seaweed extract fortified with fucoidan, characterized by adding fucoidan extracted from brown algae to seaweed extract. The seaweed extract fortified with fucoidan according to claim 1, wherein the brown algae are seaweed spores. After the salt is removed, the dried seaweed or fresh seaweed is roughly crushed, and the crushed raw material is placed in a filter bag in an appropriate amount. After pouring at a rate of 5l ~ 10l, boils while circulating water in the extractor for 30 minutes to 240 minutes at a temperature of 80 ℃ ~ 120 ℃, and then solid-liquid separation to obtain an extract of seaweed extract; After crushing the dried spores, put the crushed spores in an appropriate amount in a filter bag, put them in a pressure extractor, pour water at a rate of 7-15 l of water per 1 kg of dried spores, and adjust the pH to 2-4. Then, boil while circulating water in the extractor for 90 minutes to 240 minutes at a temperature of 80 ℃ ~ 120 ℃, the solid-liquid separation to obtain an extract to obtain the extract; A mixing step of mixing the seaweed extract and the fucoidan extract in a predetermined ratio; Fucoidan reinforcement of seaweed extract, characterized in that it comprises a. The method of claim 3, further comprising removing the pigment and seaweed odor from the seaweed extract and the fucoidan extract.