KR20090053209A - Aerosol spray with rapid microbial effects - Google Patents

Abstract

The present invention relates to a bactericidal composition comprising ethanol, an antibacterial agent p -chloro- m -cresol having a long-acting and fast-acting effect and diiodomethyl- p -tolylsulfone which is an antifungal agent, and a sterilization method using the same. The disinfectant composition of the spray formulation not only has a synergistic effect against mold and bacteria, but also is suitable as a method for home or rapid antimicrobial activity with high fast-acting effects, and it is contaminated by bacteria, fungi or viruses. The bactericidal power for the environment is excellent.

Disinfectant, aerosol spray, cresol, mold, bacteria

Description

Antibacterial aerosol spray with antiseptic fastening {AEROSOL SPRAY WITH RAPID MICROBIAL EFFECTS}

The present invention relates to an antimicrobial aerosol spray having a fast disinfecting effect, wherein the disinfectant composition according to the present invention is 70 to 99% by weight of ethanol for fast-acting sterilization, and an antibacterial agent p -chloro- m -cresol having sustainability and fast dissipation 0.01 To 1.0% by weight and 0.01 to 1.0% by weight of the antifungal diiodomethyl- p -tolylsulfone.

Microorganisms, such as bacteria, fungi, and viruses, are mostly life-threatening creatures, causing problems such as disease, odor, and aesthetic dislike. In particular, molds live in damp underground or enclosed spaces, create odors, and deform objects, causing aesthetics and disease. In addition, bacteria grow rapidly, causing odors and other diseases such as food poisoning and pneumonia.

There are many antimicrobial products to control such microorganisms, but only one of the fungi or bacteria is excellent or fast-acting, but sometimes less durable. In addition to providing an aerosol spray to compensate for these shortcomings, the spray composition must be less toxic to the human body, and must have the necessary conditions such as high activity even with a small amount of antimicrobial agent.

p -Chloro- m -cresol is a fast-acting antibacterial agent that has a rapid fast-acting effect in a small amount and is low in toxicity to the human body and is also effective in viruses, and is an antimicrobial agent that can reduce the damage of the human body by microorganisms.

Diiodomethyl- p -tolylsulfone is an antimicrobial agent that has no fast-acting but excellent sterilizing ability against mold and has a long-lasting germicidal effect.

The present invention provides a bactericidal composition comprising an antibacterial agent p -chloro- m -cresol having a synergistic effect against fungi and bacteria and an antibacterial agent diiodomethyl- p -tolylsulfone, and It is an object to provide a sterilization method used.

Another object of the present invention can be said to be suitable as a method for home or fast antibacterial effect with high fast-acting, sterilization spray having an excellent effect on the living environment contaminated by bacteria, fungi, or viruses To provide the first.

In order to achieve the above object, the present invention provides a) 70 to 99% strength by weight aqueous solution of ethanol, b) p - chloro - m - cresol, and c) di-iodomethyl - p - tolyl sulfone (p Diiodomethyl- -tolylsulphone) It includes, and provides a weight ratio of the component a), component b) and component c) 70 to 99: 1 ~ 0.01: 1 ~ 0.01 to provide an antimicrobial composition.

Hereinafter, the present invention will be described in more detail.

In the present invention, ethanol and p -chloro- m -cresol represented by Chemical Formula 1 (hereinafter PCMC) and diiodomethyl- p -tolylsulfone (hereinafter referred to as DPT) represented by Chemical Formula 2 are 70 to 99: 1 to 0.01. It relates to a fungicide composition comprising a weight ratio of 1: 1 to 0.01 and an antibacterial aerosol spray containing the same.

PCMC represented by the formula (1) is a white powder, it is not soluble in water, but has a property that is well soluble in a solvent such as alcohol. The PCMC is a disinfectant used in public buildings for homes and hospitals, and is also used for protection from microorganisms of fibers and leather. PCMC has a high initial bactericidal effect when used alone, excellent efficacy against bacteria but weak efficacy against mold, and also has a poor sustainability. The use of DPT to compensate for this disadvantage can increase the antimicrobial activity against the fungus, and improve the persistence.

In addition, the present invention can be prepared in the spray formulation of the sterilization composition, preferably an aerosol spray comprising a gas propellant.

The fungicide composition includes a solvent for dissolving the fungicide, and the solvent may be ethylene glycol, propylene glycol, alcohol, or the like, but it is preferable to use ethanol as the aerosol spray.

Various gaseous hydrocarbons may be used as the gas propellant. For this use, "hydrocarbon" has only carbon and hydrogen. They are typically liquefied under pressurized conditions in aerosol cans to become part of the hydrocarbon solvent. For example, the propellant may be dimethyl ether, difluoroethane, propane, butane, isobutane and mixtures thereof, with a mixture of isobutane and propane being preferred. More preferred propellants include A-46 (A-46) supplied by Phillips Petroleum, an 80/20 mole percent propane / isobutane mixture. Alternatively, the propellant can be another form of gas, such as carbon dioxide.

In general, the composition and preparation method of the spray formulation is a solvent for dissolving the antimicrobial agent in a can containing a solution containing 1 to 4% by weight of the antimicrobial agent in the can is sealed and filled with a gas propellant such as butane through a nozzle. It is also desirable to use surfactants or UV stabilizers to prevent corrosion of the can and discoloration due to antimicrobial agents.

Aerosol spray of a composition comprising ethanol and PCMC and DPT according to the present invention has a synergistic effect against mold and bacteria as well as a quick effect in fast-acting, which is a method for home or fast antibacterial efficacy It can be said that it is suitable, and the bactericidal power of the surrounding living environment contaminated by bacteria, fungus, or virus has an excellent effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited by these examples.

Comparative Example 1: Antimicrobial Activity of p -Chloro- m -cresol

The fungicide used in Comparative Example 1 was used by diluting PCMC (Lanxess) in an ethanol solvent at a concentration of 1% by weight.

In this experiment, Escherichia coli ATCC 11229, Micrococcus luteus ATCC 9341, Pseudomonas aeruginosa ATCC 15442, Klebsiella pneumoniae ATCC 1560, Staphylococcus aureus Five species of Reus (Staphylococcus aureus ATCC 6538) and Bacillus subtilis ATCC 6984 and Aspergillus niger ATCC 9642, Candida albicans ATCC 10231, Rhodorula Six types of fungi of Rhodotorula rubra ATCC 9449, Cryptococcus neoformans ATCC 34144, Penicillium citrinum ATCC 9849, and Trichoderma viridae ATCC 32908 The bactericidal effect of PCMC against bacteria and fungi was determined by twofold dilution method using Tryptic Soy Broth (Difco). It was steep. After incubating the culture medium containing the fungicide and microorganism for 3 days at 30 ° C., the lowest concentration of turbidity that inhibits growth was visually observed and then the minimum growth inhibitory concentration (MIC) of each fungicide or mixture thereof was observed. Was set. MIC results of the fungicides are shown in Table 1 below.

Comparative example  2 : Diiodomethyl - p - Tolyl sulfone Single Antibacterial Activity

The fungicide used in Comparative Example 2 was measured in the same manner as in Comparative Example 1, except that diiodomethyl- p -tolylsulfone (Angus) was diluted in an ethanol solvent at a concentration of 1% by weight. Is shown in Table 1 below.

division Comparative Example 1 Comparative Example 2 PCMC DPT Esherichia coli ATCC  11229 78 156 Micrococcus luteus ATCC  9341 39 312 Pseudomonas aeruginosa ATCC  15442 78 156 Klebsiella pneumoniae ATCC  1560 39 312 Staphylococcus aureus ATCC  6538 39 624 Bacillus subtilis ATCC  6984 39 156 Aspergillus niger ATCC  9642 156 19.6 Candida albicans ATCC  10231 312 19.6 Rhodotorula rubra ATCC  9449 312 9.8 Cryptococcus neoformans ATCC  34144 156 19.6 Penicillium citrinum ATCC  9849 78 9.8 Trichoderma viridae ATCC  32908 78 9.8

(Unit: ppm)

As shown in Table 1, Comparative Example 1 shows that PCMC can be controlled at low concentrations against bacteria, but it has no effect on mold compared to DPT, and DPT is relatively less effective against bacteria. . Based on the above results, it is possible to control mold and bacteria at low concentration more efficiently by compensating for the disadvantages of each substance.

Example  1: ethanol, PCMC  And DPT Antimicrobial Testing of Compositions

Based on the results obtained in Comparative Examples 1 and 2, ethanol, PCMC, and DPT were mixed at a ratio of 99.9: 0.05: 0.05% by weight to prepare a fungicide composition (Example 1), and the MIC values of the following five individual strains were measured. It was.

In the MIC test, a 96-well plate was used to dilute the fungicide according to a 2-fold serial dilution method, inoculate the microorganism at a concentration of 10 ⁴ CFU / mL, incubate for 48 hours at 30 ° C., and then turbidity of the microorganism growth. The MIC value was measured by the method of visually determining the result, and the results are shown in Table 2 below.

In order to measure the MIC value, the culture medium was observed turbidity using Tryptic Soy Broth (Difco Co.) in the same manner as in Comparative Example 1, the strain used in Example 1 Escherichia coli (Escherichia) coli ATCC 11229), Micrococcus luteus ATCC 9341, Pseudomonas aeruginosa ATCC 15442, Aspergillus niger ATCC 9642), 5 strains of Candida albicans ATCC 10231 were used.

(Unit: ppm) division Example 1 Comparative Example 1 (PCMC) Comparative Example 2 (DPT) Esherichia coli ATCC  11229 39 78 156 Micrococcus luteus ATCC  9341 39 39 312 Pseudomonas aeruginosa ATCC  15442 78 78 156 Aspergillus niger ATCC  9642 156 156 19.6 Candida albicans ATCC  10231 78 312 19.6

Through Table 2, it was found that ethanol, PCMC and DPT were mixed in a ratio of 99.9: 0.05: 0.05% by weight according to the present invention to control microorganisms as compared to the case of using a solution containing a fungicide composition alone.

Example  2: Antimicrobial Activity of Aerosol Sprays

The ethanol, PCMC and DPT obtained in Example 1 were mixed at a ratio of 99.9: 0.05: 0.05% by weight to fill the can with a disinfectant composition. Antimicrobial activity was evaluated for 30 seconds against three strains of Pseudomonas aeruginosa ATCC 15442 and Klebsiella pneumoniae ATCC 1560.

An aerosol spray filled with a disinfectant composition by spraying an area of 10 mL / m ² at a concentration of 10 ⁵ cfu / mL of individual strains on a clean stainless steel plate and mixing 99.9: 0.05: 0.05% by weight of ethanol, PCMC and DPT in an aluminum can. The growth of the microorganisms was measured 30 minutes after spraying with an amount of 5mL / m ^{2 by} a rod plate.

Comparative Example 3 was filled with the can in the same manner as the non-antibacterial composition in the can and treated in an amount of 10mL / m ² to measure the growth of microorganisms by rodak plate. The results are shown in Table 3 below and FIGS. 1 and 2.

division Example 2 Comparative Example 3 Esherichia coli ATCC  11229 No-growth Growth Micrococcus luteus ATCC  9341 No-growth Growth Pseudomonas aeruginosa ATCC  15442 No-growth Growth

Through Table 3, it was confirmed that the aerosol spray containing the fungicide composition by mixing the ethanol, PCMC and DPT in a 99.9: 0.05: 0.05% by weight ratio according to the present invention was excellent in fast-acting.

Figure 1 shows a photograph of the rod plate results of Example 3.

2 shows a photograph of the rod plate result of Comparative Example 3.

Claims (5)

a) 70 to 99% strength by weight aqueous solution of ethanol, b) p - chloro - m - cresol, and c) di-iodomethyl - p - comprises a tolyl sulfone (Diiodomethyl- p -tolylsulphone), the a) component, b The antimicrobial composition whose weight ratio of a) component and c) component is 70-99: 1-0.01: 1 -0.01. The antimicrobial composition of claim 1, wherein the fungicide composition has antimicrobial activity against both bacteria and fungi. The bacterium of claim 2, wherein the bacterium is Enterobacter aerogens, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Klebsiella pneumoniae. pneumoniae), Staphylococcus epidermis, Staphylococcus aureus, and Bacillus subtilis, and the fungus is selected from the group consisting of Aspergillus Aspergillus niger, Candida albicans, Rhodotorula rubra, Cryptococcus neoformans, Penicillium citrinum, and Trichoderma viridae Trichoderma viridae) is one or more selected from the group consisting of, antimicrobial composition. Spray comprising a bactericidal composition according to any one of claims 1 to 3. 5. An aerosol spray according to claim 4, wherein the spray comprises a gas propellant.

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