KR20090045962A - A new clonal variety with non-pollen malesterility in pusatilla species - Google Patents

Abstract

The present invention relates to a method for breeding pollen-free male sterile pasqueflower nutrient varieties, and more specifically, by immersing the chemically mutated organic source Sodium Azide at 3 mM concentration for 12 hours in 1,000 grains of pasqueflower seed, washing the seeds in clear water for 1 hour. The present invention relates to a method for breeding pollen-free male sterile pasqueflower nutrient varieties that can obtain pollen-free nutrients in plant medicines as a result of planting on general horticultural soil.

Pasqueflower, Breeding

Description

Breeding method of pollenless male sterile pasqueflower nutrient varieties {A NEW CLONAL VARIETY WITH NON-POLLEN MALESTERILITY IN PUSATILLA SPECIES}

The present invention is a male infertility nutrient with no pollen, and the characteristics are male infertility because there is no pollen, and the breeding method of pollen-free male infertility pasqueflower nutrient varieties with genetic characteristics that results in breeding when breeding as a breeding model. will be.

Pasqueflower is a perennial perennial herbaceous plant belonging to the Buttercupaceae family, and its distribution is vertically up to 900m above sea level, horizontally from Manchuria to Korea through Japan and reportedly distributed in Europe. There are four kinds of pale flowers, pink pale flowers, wild pale flowers, and thin leaf pale flowers in Korea, and Donggang pale flowers recently discovered in the Donggang basin in Gangwon-do are known, and about 30 species of pale pale flowers are distributed in Europe.

Pasqueflower flowers distributed all over the world have various gene sources such as red, reddish purple, pink, purple, yellow and white, and the height is about 20 ~ 40cm.

Pasqueflower was a common wildflower that can be easily seen anywhere in the country's wild mountains 20 years ago, but now its habitat is narrow. In addition, pasqueflower is one of the best among wildflowers in Korea, but due to lack of genetic resources, it is mainly propagated and distributed by self-cultivated farmers, and some of them grow by root cutting.

However, compared to the splendor of pasqueflower and the appreciation value of plants, the pasqueflower breeding business is not being actively activated not only domestically but also globally. As a result, there are not many varieties of pasqueflowers, and in South Korea, Jeonnam Agricultural Research and Development Institute specializes in breeding pasqueflowers. It is cultivated as a horticultural variety, and the plant genetic breeding significance of Yonhong is proud of the world's best flower field (approx. 60 cm), which shows self-incompatibility through hybridization. The new pale-flowered pasqueflower nutrient, a pink pale pasqueflower variety that symbolizes the first North-South unity cultivated by using genetic resources, was registered with the Korean Intellectual Property Office in 2006. `` Keunbi '' was patented in 2006, and purple pasqueflower 'Bulsa sapphire' in 2006 Was nurtured.

 In this situation, there is no doubt that industrialization of hobby that shows male infertility due to lack of pollen and use as a breeding model for developing new varieties will be the driving force for widening genetic resources and further activating pasqueflower breeding.

The object of the present invention in the above object is to provide a method for breeding 'hobi', a pollen-free male infertile pasqueflower nutrient varieties newly created by mutation breeding method.

In order to achieve the above object, the method of breeding pollen-free male sterile pasqueflower nutrient varieties according to the present invention, there is an artificial chemical mutation breeding method. The present invention is obtained by immersing the chemically mutated organic source Sodium Azide in 1,000 granules at 3mM for 12 hours and washing the seeds in clear water for 1 hour to sow seeds in general horticultural soil to obtain nutrients without pollen in plant medicine. It is characterized by.

At the same time, the main agricultural traits were examined for three years at the same time as the propagated nutrients were bred as described above. In addition, artificial breeding using this strain as a model resulted in the fruiting, and as a result, this botanical male sterility was identified as a botanically new genetic characteristic, and the cultivar name of the nutrient was named 'hobby'.

 The nutrients bred by the method of breeding pollen-free male infertile pasqueflower nutrient varieties according to the present invention by the above configuration is a nutrient pasqueflower which is a nutritional cultivar with the characteristics of pollen-free males infertility, a new type of pasqueflower As it is a nutrient variety that has diversified the type of flower, there is no pollen, so it is possible to enjoy clean flowers for a long time, so that it can be industrialized as a sieve, and the male sterility characteristic can be a new breeding target of pasqueflower breeding. Its use is high.

In addition, pasqueflower is the flower deeply inscribed in Korean national sentiment among native flowers and is usually used for garden or eruption. Since all of the genera in the Korean paleblossom pollen have pollen, the flowers are retracted at night during the flowering period, and the flowers are polluted on the petals by repeating the movements during the day, so the flowers do not have a clean feeling, but the hobby has no pollen, Because of its long flowering period and its ability to enjoy beautiful flowers for a long time, Hobby is a new type of flower that not only satisfies the new needs of consumers, but also newly created the morphological characteristics of flowers. As its value is large, it can be industrialized and male sterile flowers are set as breeding targets for the development of new pasqueflower varieties.

Hereinafter, a method of breeding pollen-free male infertile pasqueflower nutrient varieties according to the present invention will be described in more detail with reference to Examples.

The present invention was obtained by immersing the chemically mutated organic source Sodium Azide in 1,000 granules at 3mM for 12 hours and washing the seeds in clear water for 1 hour to sow seeds in general horticultural soil to obtain nutrients without pollen in plant medicine. .

The nutrient obtained as described above is a male sterile nutrient cultivar, which is not self-fertilized because there is no pollen, but it can be fruited when used as a hybrid model, and it is breeding as a cultivar of a double nutrient cultivar with 12 petals. The characteristics are largely distinguished from other pasqueflowers.

The following table summarizes the details of pasqueflower double flower nutrient varieties as a result obtained in the above breeding process.

Table 1 summarizes the fire and leaf characteristics of pollen-free male sterile pasqueflower nutrient varieties. According to Table 1, the petal color of pollen-free male sterile pasqueflower nutrient varieties is reddish purple.

Breed name Petal color Blooming number Color of medicine Orange Blooming direction Petals (pcs) Leaf color core End Ho rain Fuchsia Fuchsia Once a year Yellow Fuchsia Downward 6 rust Light red Pink pink Twice a year Yellow Pink Downward 6 rust

Table 2 summarizes the characteristics related to male sterility of pollen-free male sterile pasqueflower nutrient varieties. According to Table 2, male sterile pasqueflower nutrient varieties without pollen are male sterile and self-incompatible because there is no pollen and the medicine does not burst.

Breed name Male sterility Self-compatibility With or without pollen A burst of medicine Ho rain U radish radish radish Light red radish radish U U

Table 3 summarizes the flower and growth characteristics of pollen-free male sterile pasqueflower nutrient varieties. According to Table 3, male sterile pasqueflower nutrient varieties without pollen are short-flowered and short-leaved, broad-pocketed, fast flowering, and flowering each year.

Breed name Flower market (cm) Width (cm) Width (cm) Leaf length (cm) Blooming season (Mon. and Sun) Flowering water (dogs / week) Siege prize system bloom October Primary Secondary Ho rain 22.0 8.7 30.7 6.0 14.3 12.7 41.6 2.20 - 18.2 Light red 31.5 22.1 53.6 6.5 11.4 28.3 62.3 3.13 7.23 24.6

'Hobi', a male sterile nutrient without pollen, a new type of pasqueflower, is a nutrient variety created by chemical mutation breeding method and has an ornamental value not found in the existing pasqueflower gene source. No root rooting, that is, by cutting off the bottom of the nutrient tube and removing a large amount of malnutrition from the remaining roots in the soil and transplanting it to the seedlings, or when planting pots on the ground with adequate moisture 1 After 2 months, the thin roots get stuck in the ground, and if you move the pots carefully so that the roots are not pulled out of the ground, a large amount of malnutrition is generated from the roots embedded in the ground and then used as a seedling to breed large numbers of nutrients. Is efficient.

 1 is a photo showing a flower of palebloom new varieties hobby according to an embodiment of the present invention

Figure 2 is a photo showing clean flowers without pollen of paleflower new species hobby according to an embodiment of the present invention

Figure 3 is a photo showing the appearance of the pollen of the common pasqueflowers stained by petals according to an embodiment of the present invention

Figure 4 is a picture showing the medicine of a new species of pasqueflower pale in accordance with an embodiment of the present invention is not translucent because the drug looks translucent

5 is a photograph showing a general medicine of the pasqueflower according to an embodiment of the present invention is full of pollen in the medicine is dark yellow.

Figure 6 is a photograph showing that the aging without pollen of the new species of pasqueflower pale in accordance with an embodiment of the present invention aging

Figure 7 is a photo showing the appearance of pollen coming out of the medicine of a common pasqueflower according to an embodiment of the present invention

Claims (1)

Sodium Azide chemically mutated organic source Sodium Azide immersed in 1,000 grains of 1,000 P. glen seeds for 12 hours, washed with seeds for 1 hour in clear water and sown in general horticultural soil, pollen to obtain nutrients without pollen in plant medicine Breeding method of male sterile pasqueflower nutrient varieties.

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