KR20090027387A - A composition containing sargassum horneri extract for skin external application - Google Patents

Abstract

A Sargassum horneri extract is provided to improve excellently antiwrinkling and elasticity of skin and to be used as a skin preparation for external use. A Sargassum horneri extract is extracted by using water, alcohol or a mixture thereof. The alcohol is low-class alcohol of C1~C3. The Sargassum horneri extract improves aging of skin, increases activity of a collagen transcription factor and biosynthesis of the collagen in the skin cell.

Description

A composition containing Sargassum horneri extract for skin external application}

1 is a diagram showing the increase effect of collagen transcription factor (COL1A1 promotor) of the extract of boksaeng mother banja (50μg / ml), Figure 2 shows the mRNA effect of the collagen of hoesaeng banban extract (50μg / ml) Figure 3 is a view showing the effect of the expression of collagen protein by boksaeng mother's disease extract (50μg / ml).

The present invention relates to an external preparation composition for skin containing the extract of boksaeng mother. More specifically, the present invention relates to an external preparation for skin having a function of preventing the skin aging by extracting the extract obtained by extracting a hoesangja macho starch, which is brown algae with mabanban, using an appropriate solvent mixture. The present invention can be prepared in the form having a flexible cosmetic water, nourishing cosmetics, massage cream, nourishing cream, pack, gel, essence or skin adhesive type cosmetics, to provide wrinkle improvement, elasticity improvement effect on the skin.

Skin aging can be divided into endogenous aging process that occurs according to the natural aging process of living organisms that are informed on genes, and exogenous aging (photoaging) caused by the deposition of wrinkles and pigments by exposure to ultraviolet rays contained in light such as the sun. have. The mechanism of skin aging begins with the loss of collagen in the dermis. The loss of collagen is due to the increased activity of collagen degrading enzyme Matrix Metalloproteinase (MMP), which causes collagen breakdown. Therefore, if you inhibit the collagen decomposition process can be expected to suppress or slow down the skin aging. Based on this principle, the method of inhibiting skin aging is to promote collagen synthesis or to inhibit collagen degradation. Specifically, in the case of collagen production, skin is promoted by promoting an increase in the transcription factor involved in the synthesis of collagen, inducing a quantitative increase in mRNA that synthesizes collagen, or by promoting the synthesis of collagen protein from mRNA. It can suppress aging. The method of inhibiting collagen degradation may be induced by tissue inhibitor metalloproteniase (TIMP), another protein that inhibits MMP activity or inhibits MMP activity. The present invention provides a skin external preparation having a function of inhibiting skin aging by activating a factor that is involved in the process of promoting the production of collagen extract.

Hokkaeng mother's bed is 2-5m long perennial seaweed that grows on reef. With a tray-shaped hut, a cylindrical stem comes out from the center and strikes a side branch. The leaves are long oval or stripe-shaped and split into feather-shaped edges. It is used as feed or food, and is distributed all over Korea and Japan.

A study on the ugly maternal babies showed that the ugly yam extract was effective in preventing osteoporosis ( Yakugaku Zasshi. 2006 , 126 , 1117-1137), and was effective in preventing blood coagulation ( Bioresour. Technol. 2007 , 98, 1711-1716.). It has also been reported that the hot water extract of P. aeruginosa has the inhibitory effect of herpes simplex virus type 1. ( Chem. Pharm. Bull. 2001 , 49 , 484-485 . ; Biol. Pharm. Bull. 1998 , 21 , 730-734.).

However, much research has not been conducted on the active substances contained in the hoeban group, and fucan sulfate and sulfate polysaccharides, called sodium hornan, have been reported. Looking at the patent for the boksaeng mother's maternity bed, Korea's hoesan mother's bed extract was manufactured as a food additive, which is registered as having an anticancer effect on Hela cells, cervical cancer cells. There are many patents for hoesan mother-in-law in Japan. Patents for anti-aging (JP2007-119384), angiogenesis inhibitors (JP2006-022033), external skin preparations (JP2004-067593), collagen production promoters (JP1998-330280) are registered.

The inventors of the present invention showed that the extract of the boksaeng mother's nest of seaweeds growing in the sea showed the effect of increasing the collagen transcription factor (COL1A1 promotor), the mRNA amount of collagen, and eventually the collagen protein was expressed. The invention has been completed.

An object of the present invention is to provide a new use as a skin external preparation for the skin wrinkles and elasticity by inducing the biosynthesis of collagen extracts.

In order to achieve the above object, the present invention has an anti-wrinkle function, and provides an extract of boksaeng majaban extracted with water, alcohol or a mixture thereof.

In addition, the present invention has an elasticity improving function, and provides an extract of boksaeng mother's head extracted with water, alcohol or a mixture thereof.

In addition, the present invention provides a cosmetic for improving skin aging comprising the extract of boksaeng mother.

In addition, the present invention provides a cosmetic for improving wrinkles comprising the extract of boksaeng mother.

In addition, it provides a resilient improvement cosmetics containing the extract of boksaeng mother.

Hereinafter, the present invention will be described in detail.

The present invention provides an extract of the boksaeng mother's skin having a function of improving skin aging.

The present inventors extracted the dried boksaeng mother's field outpost to investigate the effect of the boksaeng mother's field extract on skin aging improvement. The method of extracting the boksaeng mamopan may be used by a general extraction method known in the art, and extracted by the following extraction methods: 1) obtaining an extract from the boksaeng mabanban using water, alcohol or a mixture thereof; 2) filtering the extract of step 1); 3) concentrating the solution of step 2). The alcohol in step 1) is preferably 100% methanol, preferably 5 to 10 times the volume. The extraction time is preferably 2 to 24 hours, most preferably 3 hours.

  The extract of boksan emojiban showed an excellent effect of increasing the collagen transcription factor (COL1A1 promotor) (see Fig. 1), the mRNA increase of collagen (see Fig. 2) and collagen biosynthesis (see Fig. 3). Through this, the hoesaengjaban extract can be usefully used as an effective skin external preparation that can improve the wrinkles and elasticity of the skin by promoting collagen biosynthesis of the dermal layer in the skin.

The present invention provides cosmetics for anti-aging comprising the extract of the boksaeng mother's skin having wrinkles and elasticity improvement function.

In preparing the anti-aging cosmetics containing the extract of boksaeng mother's skin of the present invention, 0.01 to 15 parts by weight, preferably 0.1 or 10 parts by weight of the hoesin mother's extract of the present invention may be added to the composition for external application for skin. Can be. The skin external preparation of the present invention includes a fatty extract, an organic solvent, a dissolving agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, a fragrance, a surfactant, and a water in addition to the extracts Commonly used in ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or external preparations for skin And any other ingredients that may be used, such as adjuvants conventionally used in the field of dermatology. The ingredients may also be introduced in amounts generally used in the field of dermatology.

The hoesangjapan extract, which functions to improve the skin aging of the present invention, shows an excellent effect of increasing collagen transcription factor (COL1A1 promotor) (see FIG. 1), an increase in collagen mRNA amount (see FIG. 2) and an increase in collagen biosynthesis ( 3 is not particularly limited in the formulation, for example, a cosmetic composition having a formulation of a flexible cosmetic water, nourishing cosmetics, massage cream, nutrition cream, pack, gel or skin adhesion type cosmetic table It may be used in a transdermal dosage form such as lotion, ointment, gel, cream, patch or spray.

The extract of hoesan mother-of-law having a function of improving skin aging of the present invention may further contain one or more active ingredients exhibiting the same or similar functions.

In addition to the above-mentioned main components, the hoesin capillary extract having the function of improving skin aging of the present invention is a mineral-containing compound such as vitamin B, C, E or beta-carotene, Ca, Mg, Zn or phospholipids such as lecithin or maltol, oligosaccharides. Compounds such as amino acids and the like can be used. Among them, the use of 2-3 components in vitamin C, E, beta-carotene, maltol, etc. is more preferable because it can enhance the biological activity effect.

Hereinafter, the present invention will be described in detail by Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Examples.

Example 1 Preparation of Boksaeng Mother Hat Extract

1.0 Kg of wet-weak mother's cap in frozen state was crushed, immersed in 3L of 80% aqueous methanol solution, stirred for 24 hours, and filtered through a filter paper at room temperature. The extract was concentrated using a concentrator under reduced pressure until methanol completely evaporated, then 150 mL of water was added and extracted three times with 200 mL of ethyl acetate. Magnesium sulfide was added to the extracted ethyl acetate to remove moisture, and after filtration, all ethyl acetate was evaporated by vacuum distillation. As a result of using the above method, 4.8 g of the extract can be prepared from 1 kg of dried boksamae macho outpost.

Experimental Example 1 Increase of Collagen Transcription Factor (COL1A1 Promotor)

In order to confirm the effect of increasing the collagen transcription factor of the extract of boksaeng mother's eves in Example 1 prepared by the above method, a reporter gene assay using plasmid conjugated with luciferase was performed. Briefly, NIH-3T3 cell lines are seeded in 24-well plates and incubated for 24 hours in DMEM medium containing 10% fetal calf serum and 1% penicillin / streptomycin. After the transcription factor was activated, COLlA1 promoter-luciferase reporter plasmid DNA (pGL3-Basic, Promega), a plasmid designed to report it as luciferase, was transfected with Lipofectamin transfection reagent (Invitrogen). Twenty four hours after transfection, the extract was treated with 50 µg / ml concentration, and after 36 hours, cells were lysed and luciferase activity was measured by luminometer to measure COL1A1 promoter transcription factor activity.

Experimental Example 2 Measurement of Expression of the Collagen Gene COL1A1 (In vitro)

NIH-3T3 was incubated in 6-well plates at 10 ⁵ concentrations and treated with algae extracts for 36 hours after 24 hours. After 36 hours of seaweed extract treatment, the cells were collected and subjected to reverse transcription PCR (RT-PCR) to quantify the mRNA of the transcribed COL1A1.

Experimental Example 3 Measurement of Collagen Biosynthesis Efficacy in Skin Cells

NIH-3T3 cells were cultured in 24 well plates at a concentration of 3 × 10 ⁴ , and after 24 hours, the extracts were treated and cultured for 36 hours. After 36 hours of extract treatment, cells were washed with PBS and treated with a surfactant and a protease inhibitor to obtain a cell homogenate. The cell homogenate was electrophoresed and trasfered to nitrocellulose membrane, and the expression level of collagen protein was measured by immunbloting with secondary antibody combined with primary antibody and peroxidase against collgen.

As described above, the extract of boksan motzaban, which is extracted with water, alcohol or a mixture thereof, increases the activity of the transcription factor of the collagen transcription factor (COL1A1 promotor), increases the mRNA of the collagen gene COL1A1, and collagen in skin cells. By increasing the biosynthesis to increase the collagen which is the main constituent of the skin, there is an effect of inhibiting inflammation, improving wrinkles, improving elasticity, so it can be used as an external preparation for skin such as cosmetics.

Claims (7)

An ugsang majaban extract having skin aging improvement function and extracted with water, alcohol or a mixture thereof. Boksaeng Mamopan extract extracted with water, alcohol or mixtures thereof to increase the activity of the transcription factor of the collagen transcription factor (COL1A1 promotor). An extract of Boksaeng Majaban, which is extracted with water, alcohol or a mixture thereof which increases the mRNA of the collagen gene COL1A1 and improves skin aging. An extract of boksaeng mother's disease extracted from water, alcohol or a mixture thereof which increases collagen biosynthesis in skin cells to increase collagen, a major component of skin. The method of claim 1, wherein the alcohol is C ₁ to C ₃ lower alcohol, characterized in that the boksaeng mazaban extract. External preparation for improving wrinkles, including hoesan mother-child extract as an active ingredient. External preparation for elasticity improvement including boksaeng mother's cap extract as an active ingredient.

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