KR20080087952A - Filter for water purifier including organic germanium and thereof method - Google Patents
Filter for water purifier including organic germanium and thereof method Download PDFInfo
- Publication number
- KR20080087952A KR20080087952A KR1020070030127A KR20070030127A KR20080087952A KR 20080087952 A KR20080087952 A KR 20080087952A KR 1020070030127 A KR1020070030127 A KR 1020070030127A KR 20070030127 A KR20070030127 A KR 20070030127A KR 20080087952 A KR20080087952 A KR 20080087952A
- Authority
- KR
- South Korea
- Prior art keywords
- organic germanium
- water
- filter
- porous adsorbent
- test
- Prior art date
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 105
- 229910052732 germanium Inorganic materials 0.000 title claims abstract description 82
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000003463 adsorbent Substances 0.000 claims abstract description 48
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- 229920000642 polymer Polymers 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000001856 Ethyl cellulose Substances 0.000 claims abstract description 18
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000012153 distilled water Substances 0.000 claims abstract description 18
- 235000019325 ethyl cellulose Nutrition 0.000 claims abstract description 18
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- 238000000576 coating method Methods 0.000 claims abstract description 17
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- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 claims description 3
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
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- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
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Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/288—Treatment of water, waste water, or sewage by sorption using composite sorbents, e.g. coated, impregnated, multi-layered
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/10—Filtering material manufacturing
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Treatment By Sorption (AREA)
Abstract
Description
도 1은 본 발명에 따른 유기게르마늄을 포함한 정수기용 필터의 제조공정도를 도시한 도면.1 is a view showing a manufacturing process of the filter for water purifiers containing organic germanium according to the present invention.
본 발명은 유기게르마늄을 포함하는 정수기용 필터 및 상기 필터의 제조방법에 관한 것이다.The present invention relates to a filter for water purifier containing organic germanium and a method for producing the filter.
최근 산업발전 속도가 급속히 증가함에 따라 각종 공장설비 등으로부터 발생되는 산업폐수 등이 식수원에 유입되어 수질이 저하되고 있다. 통상적으로 각 가정에 공급되는 수돗물은 각 하천의 수계에서 취수장을 통하여 취수되어 저수장에서 오염된 물을 오염 및 정수과정을 거쳐 상수도관을 통하여 공급되고 있으나, 정수과정에서 다량의 소독약품을 사용한 결과 별도의 처리없이 취음할 경우 오취로 인한 문제점이 발생한다. 이에 따라, 현재 생수 또는 정수된 물을 식용으로 하는 경향이 일반화되고 있다. 그러나, 생수 또는 정수된 물은 쉽게 미생물로 인해 오염되기 쉽고 이취를 발생한다.In recent years, as the speed of industrial development increases rapidly, industrial wastewater generated from various plant facilities, etc., is introduced into a drinking water source, thereby degrading water quality. Normally, tap water supplied to each household is withdrawn from the water system of each stream through the intake station, and the contaminated water from the reservoir is supplied through the water pipe through the pollution and water purification process, but as a result of using a large amount of disinfectant in the water purification process If you drink without any treatment, problems with odor occurs. Accordingly, the tendency to make drinking water or purified water edible is common. However, bottled or purified water is easily contaminated by microorganisms and generates off-flavor.
따라서 상기와 같은 문제점 등을 해결하기 위해 수조가 합성수지제로 형성되고 미세필터에 의하여 정수시키는 정수기를 사용한다. 상기와 같은 정수기는 미네랄 등의 인체에 이로운 성분을 더욱 활성화시킬 수 없을 뿐만 아니라 매우 불량한 대장균 제거작용과 살균작용 및 탈취작용에 의하여 건강을 해치게 되는 문제가 따르게 되었다.Therefore, in order to solve the above problems and the like, a water tank is formed of a synthetic resin and uses a water purifier purified by a fine filter. The water purifier as described above is not only able to further activate the beneficial components such as minerals, but also has a problem that harms the health by very poor E. coli removal and sterilization and deodorizing action.
상기와 같은 여러 문제점을 해결하기 위해 발명된 것으로 등록특허공보 10-0360283호에는 대나무 활성탄 여과층, 참나무 활성탄 여과층, 소나무의 활성탄 여과층, 맥반석 여과층 및 게르마늄광석 여과층을 다단계로 적층하거나, 상기 재료를 일정비율로 혼합하여 제작한 필터를 이용한 활성 정수기구를 개시한다.Patent No. 10-0360283 has been invented to solve the various problems as described above is laminated bamboo activated carbon filtration layer, oak activated carbon filtration layer, activated carbon filtration layer of pine, ganban stone filtration layer and germanium ore filtration layer in multiple stages, Disclosed is an active water purification device using a filter prepared by mixing the above materials at a constant ratio.
상기와 같은 정수기구는 수중 유해물질을 제거하고 하천수의 제2오염을 방지하는 장점은 있으나, 여과필터가 다단계로 형성되어 있어서 각 재료를 여과층으로 처리하는 공정이 요구되며, 상기와 같이 다단으로 형성된 여러 여과층을 통과하여 오히려 물에 함유된 유익한 미네랄도 함께 여과되는 문제가 있다.The water purification device as described above has the advantage of removing harmful substances in the water and preventing the second pollution of the river water, but the filtration filter is formed in multiple stages, the process of treating each material with a filtration layer is required, formed in multiple stages as described above The problem is that the beneficial minerals contained in the water are also filtered through several layers of filtration.
상기 문제점을 해결하기 위한 본 발명의 목적은 정수기에서 정수시에 유기게르마늄을 용출시키기 위해서 다공성 흡착제에 유기게르마늄을 흡착시켜 불용성 고분자로 코팅된 것을 특징으로 하는 유기게르마늄을 포함하는 정수기용 필터와 상기 정수기용 필터의 제조방법을 제공하고자 한다.An object of the present invention for solving the above problems is to filter the water purifier and the organic water purifier comprising an organic germanium, characterized in that the organic germanium is adsorbed on the porous adsorbent in order to elute the organic germanium at the purified water It is to provide a method for producing a filter for.
상기 목적을 달성하기 위한 본 발명은 유기게르마늄을 포함하는 정수기용 필 터 및 상기 정수기용 필터의 제조방법에 관한 것이다.The present invention for achieving the above object relates to a filter for a water purifier containing an organic germanium and a method for producing the filter for water purifiers.
보다 상세하게는 다공성 흡착제에 유기게르마늄을 흡착시켜 불용성 고분자로 코팅된 것을 특징으로 하는 유기게르마늄을 포함하는 정수기용 필터를 특징으로 한다.More specifically, it characterized by a filter for water purifiers containing organic germanium, characterized in that the organic germanium is adsorbed on the porous adsorbent and coated with an insoluble polymer.
또한 본 발명은 ⑴ 다공성 흡착제를 70 ~ 150 ℃ 에서 1 ~ 2 시간 가열하는 단계;In another aspect, the present invention comprises the step of heating the porous adsorbent at 70 ~ 150 ℃ for 1 to 2 hours;
⑵ 유기게르마늄을 증류수에 용해시키는 단계;⑵ dissolving organic germanium in distilled water;
⑶ ⑴ 단계에서 가열된 다공성 흡착제에 ⑵ 단계에서 유기게르마늄이 용해된 물을 혼합하는 단계; 및Mixing water in which the organic germanium is dissolved in step iv with the porous adsorbent heated in step iii; And
⑷ ⑶ 단계에서 혼합된 유기게르마늄이 용해된 물을 25 ~ 100 ℃ 에서 12 ~ 24 시간 건조시키는 단계로 이루어진 다공성 흡착제에 유기게르마늄을 흡착시키는 흡착공정과,An adsorption process of adsorbing the organic germanium on the porous adsorbent, which comprises drying the mixed water of the organic germanium mixed in the step ⑷ 에서 at 25 to 100 ° C. for 12 to 24 hours,
⑸ 에틸 알코올에 불용성 고분자를 용해시킨 후 증류수를 첨가하여 불용성 고분자 코팅용액을 제조하는 단계;단계 preparing an insoluble polymer coating solution by dissolving insoluble polymer in ethyl alcohol and then adding distilled water;
⑹ ⑷ 단계에서 유기게르마늄이 흡착된 다공성 흡착제를 불용성 고분자 코팅용액로 코팅하는 단계; 및Coating a porous adsorbent on which the organic germanium is adsorbed in the step ⑹ ⑹ with an insoluble polymer coating solution; And
⑺ ⑹ 단계에서 코팅된 다공성 흡착제를 70 ~ 80 ℃ 에서 12 ~ 24 시간 건조시키는 단계로 이루어진 코팅공정을 포함하는 것을 특징으로 하는 유기게르마늄을 포함하는 정수기용 필터의 제조방법을 특징으로 한다.Characterized in that the method for producing a filter for water purifier comprising an organic germanium, characterized in that it comprises a coating process comprising the step of drying the coated porous adsorbent in step ⑺ 70 at 70 ~ 80 ℃ 12 to 24 hours.
또한 본 발명은 상기 다공성 흡착제로 활성탄, 실리카, 활성 알루미나, 제올 라이트 및 필라드클레이로부터 어느 하나 선택되는 것을 특징으로 한다.In addition, the present invention is characterized in that any one selected from activated carbon, silica, activated alumina, zeolite and filad clay as the porous adsorbent.
또한 본 발명은 상기 불용성 고분자로 에틸셀룰로오스, 질산셀룰로오스, 초산셀룰로오스 및 프로피온산셀룰로오스로부터 어느 하나 선택되는 것을 특징으로 한다.In addition, the present invention is characterized in that any one selected from ethyl cellulose, cellulose nitrate, cellulose acetate and cellulose propionate as the insoluble polymer.
또한 본 발명은 상기 불용성 고분자의 첨가량이 15 ~ 25 g인 것을 특징으로 한다.In addition, the present invention is characterized in that the addition amount of the insoluble polymer is 15 to 25 g.
또한 본 발명은 상기 다공성 흡착제의 첨가량이 5 ~ 10 ㎏인 것을 특징으로 한다.In addition, the present invention is characterized in that the addition amount of the porous adsorbent is 5 ~ 10 kg.
또한 본 발명은 상기 유기게르마늄의 첨가량이 5 ~ 10 g인 것을 특징으로 한다.In addition, the present invention is characterized in that the addition amount of the organic germanium is 5 ~ 10 g.
또한 본 발명은 상기 에틸 알코올의 첨가량이 5 ~ 10 ℓ인 것을 특징으로 한다.In addition, the present invention is characterized in that the addition amount of the ethyl alcohol is 5 ~ 10L.
이하 본 발명을 보다 상세히 설명하기로 한다.Hereinafter, the present invention will be described in more detail.
본 발명은 다공성 흡착제를 70 ~ 150 ℃에서 1 ~ 2시간 동안 가열함으로써 활성탄의 기공을 확장하여 유기게르마늄의 흡착을 용이하게 하는데, 이것은 활성탄에 유기게르마늄을 상온에서 흡착시킬 때 보다 가열하여 게르마늄을 흡착시킬 때가 초기의 게르마늄 용출량을 감소시켜 정수시에 유기게르마늄이 지속적으로 용출시키는데 바람직하기 때문이다.The present invention facilitates the adsorption of organic germanium by expanding the pores of activated carbon by heating the porous adsorbent at 70 ~ 150 ℃ for 1 to 2 hours, which is heated to adsorb germanium to the activated carbon than at room temperature This is because it is preferable to reduce the initial germanium elution amount so that the organic germanium is continuously eluted at the time of purification.
상기 다공성 흡착제에 유기게르마늄을 흡착시키기 위하여 고체상의 유기게르마늄을 액상으로 제조하기 위해 증류수 5 내지 15 ℓ에 용해시킨다.In order to adsorb the organic germanium to the porous adsorbent, a solid organic germanium is dissolved in 5 to 15 L of distilled water to prepare a liquid phase.
본 발명은 다공성 흡착제와 유기게르마늄의 혼합액을 25 ~ 100 ℃ 에서 12 ~ 24 시간 건조시키는데, 이러한 단계는 다공성 흡착제에 흡착된 유기게르마늄을 안정화시키고 수분을 제거하기 위해서 수행한다. 바람직하게는 70 ℃에서 건조되는 것이 바람직한데, 이것은 건조시간을 단축시키고 다공성 흡착제를 110 ℃에서 가열하여 확장된 흡착제의 기공을 축소시키기 위해서 바람직할 수 있다.The present invention is to dry the mixture of the porous adsorbent and organic germanium for 12 to 24 hours at 25 ~ 100 ℃, this step is carried out to stabilize the organic germanium adsorbed on the porous adsorbent and remove moisture. Preferably, it is preferably dried at 70 ° C., which may be desirable to shorten the drying time and to reduce the pores of the expanded adsorbent by heating the porous adsorbent at 110 ° C.
본 발명은 불용성 고분자 코팅용액을 제조시에 에틸 알코올과 에틸셀룰로오스와 증류수를 첨가하는데, 이때 에틸 알코올의 첨가량은 5 ~ 10 ℓ이고, 불용성 고분자의 첨가량은 15 ~ 25 g이고, 증류수의 첨가량은 1 ~ 8 ℓ이다.The present invention adds ethyl alcohol, ethyl cellulose and distilled water when preparing an insoluble polymer coating solution, wherein the amount of ethyl alcohol is 5-10 L, the amount of insoluble polymer is 15-25 g, and the amount of distilled water is 1 ˜8 L.
본 발명은 유기게르마늄을 흡착시킨 다공성 흡착제와 에틸셀룰로오스의 혼합액에서 알코올을 제거하기 위해서 70 ~ 80 ℃ 에서 12 ~ 24 시간 가열한다. 바람직하게는 60 ℃에서 가열하는데, 이것은 에틸셀룰로오스로 코팅된 유기게르마늄이 흡착된 다공성 흡착제를 필터로 사용하기 위해 완전히 건조시키는 단계로 과도한 온도를 유지할 경우, 알코올의 발화가 발생될 수 있으며, 상온에서 가열하여 건조할 경우 건조시간이 길어지는 공정상 단점이 있어 60 ℃에서 가열하여 건조하는 것이 바람직함을 확인하였다.The present invention is heated for 12 to 24 hours at 70 ~ 80 ℃ to remove the alcohol from the mixture of the porous adsorbent and ethyl cellulose adsorbed organic germanium. It is preferably heated at 60 ° C., which is a step of completely drying the porous organic adsorbent coated with ethyl cellulose to be used as a filter. If excessive temperature is maintained, ignition of alcohol may occur, and at room temperature When drying by heating, there is a disadvantage in that the drying time is long, it was confirmed that drying by heating at 60 ℃.
이하 일 실시예를 통해 본 발명을 보다 상세히 설명하기로 한다.Hereinafter, the present invention will be described in more detail with reference to one embodiment.
실시예 1Example 1
활성탄 7 ㎏을 110 ℃ 에서 1시간 30분 가열하여 상기 활성탄의 기공을 확장시키고 유기게르마늄 7 g을 증류수 11 ℓ에 용해시켰다. 가열된 활성탄에 유기게르마늄이 용해된 물 11 ℓ를 혼합하였다. 상기 혼합된 유기게르마늄이 용해된 물을 70 ℃ 에서 24 시간 건조시켜 활성탄의 확장된 기공에 흡착시켰다.7 kg of activated carbon was heated at 110 ° C. for 1 hour and 30 minutes to expand pores of the activated carbon, and 7 g of organic germanium was dissolved in 11 L of distilled water. 11 liters of water in which organic germanium was dissolved was mixed with the heated activated carbon. The mixed organic germanium-dissolved water was dried at 70 ° C. for 24 hours to adsorb the expanded pores of activated carbon.
비교예 1Comparative Example 1
상기 실시예에서 활성탄을 110 ℃로 가열하지 않고 상온에서 유기 게르마늄을 흡착시키는 것을 제외하고는 실시예 1과 동일하게 수행하였다.In the above embodiment was carried out in the same manner as in Example 1 except that the activated carbon is adsorbed at room temperature without heating the activated carbon to 110 ℃.
실험예 1Experimental Example 1
상기 실시예 1 및 비교예 1에서 실시된 각각의 유기게르마늄이 흡착된 활성탄 70 g을 가정용 정수기의 필터에 충진 후에 증류수를 각각 1 ℓ, 20 ℓ, 100 ℓ, 300 ℓ, 500 ℓ, 1000 ℓ 및 1500 ℓ를 통과시켜 게르마늄의 용출량을 비교하였다.After filling the filter of the domestic water purifier with 70 g of the activated carbon adsorbed to each organic germanium carried out in Example 1 and Comparative Example 1, 1 L, 20 L, 100 L, 300 L, 500 L, 1000 L and 1500 L was passed through to compare the elution of germanium.
상기 실험예 1에 따른 유기게르마늄 용출량을 이하의 표 1에 나타낸다.The organic germanium elution amount according to Experimental Example 1 is shown in Table 1 below.
상기 표 1에 나타낸 바와 같이 본 발명에 따른 실시예 1에서는 정수기에서 정수 시에 증류수 1 ℓ를 활성탄을 110 ℃에서 가열하여 유기게르마늄을 흡착시킨 필터를 통해 통과시킨 경우가 상온에서 유기게르마늄을 흡착시킨 경우보다 유기게르마늄 용출량이 적게 용출되었으나, 필터의 통과량이 많아질수록 유기게르마늄의 용출량이 증가하였다.As shown in Table 1, in Example 1 according to the present invention, when 1 L of distilled water was heated at 110 ° C. in a water purifier and then passed through a filter in which organic carbon was adsorbed, the organic germanium was adsorbed at room temperature. The amount of organic germanium eluted was less than that of the case, but the amount of organic germanium eluted increased as the amount passed through the filter increased.
상기와 같은 결과는 상온에서 다공성 흡착제에 유기게르마늄을 흡착시키는 것 보다는 고온, 즉 110 ℃에서 다공성 흡착제를 가열하여 기공을 확대시켜서 유기게르마늄이 다공성 흡착제의 확대된 기공 속으로 흡착되기 때문인 것으로 유추할 수 있었다.These results can be inferred to be due to the expansion of pores by heating the porous adsorbent at a high temperature, ie, 110 ° C., rather than adsorbing organic germanium to the porous adsorbent at room temperature, thereby adsorbing the organic germanium into the enlarged pores of the porous adsorbent. there was.
실시예 2Example 2
활성탄 7 ㎏을 110 ℃ 에서 1시간 30분 가열하여 상기 활성탄의 기공을 확장시키고 유기게르마늄 7 g을 증류수 11 ℓ에 용해시켰다. 가열된 활성탄에 유기게르마늄이 용해된 물 11 ℓ를 혼합하였다. 상기 혼합된 유기게르마늄이 용해된 물을 70 ℃ 에서 24 시간 건조시켜 활성탄의 확장된 기공에 흡착시켰다. 에틸 알코올 8.5 ℓ에 에틸셀룰로오스 21 g을 용해시킨 후 증류수 4.5 ℓ를 첨가하여 에틸셀룰로오스 코팅용액 13 ℓ를 제조하였다. 상기 유기게르마늄이 흡착된 활성탄에 에틸셀룰로오즈 코팅용액 11 ℓ를 코팅하고 75 ℃ 에서 20 시간 건조시켰다.7 kg of activated carbon was heated at 110 ° C. for 1 hour and 30 minutes to expand pores of the activated carbon, and 7 g of organic germanium was dissolved in 11 L of distilled water. 11 liters of water in which organic germanium was dissolved was mixed with the heated activated carbon. The mixed organic germanium-dissolved water was dried at 70 ° C. for 24 hours to adsorb the expanded pores of activated carbon. After dissolving 21 g of ethyl cellulose in 8.5 L of ethyl alcohol, 4.5 L of distilled water was added to prepare 13 L of an ethyl cellulose coating solution. 11 l of an ethyl cellulose coating solution was coated on the activated carbon to which the organic germanium was adsorbed, and dried at 75 ° C. for 20 hours.
비교예 2Comparative Example 2
상기 실시예 2에서 에틸셀룰로오스 7 g을 사용하는 것을 제외하고 실시예와 동일하게 수행하였다.The same procedure as in Example 2 was repeated except that 7 g of ethyl cellulose was used.
비교예 3Comparative Example 3
상기 실시예 2에서 에틸셀룰로오스 35 g을 사용하는 것을 제외하고 실시예 2와 동일하게 수행하였다.Except for using 35 g of ethyl cellulose in Example 2 was carried out in the same manner as in Example 2.
실험예 2Experimental Example 2
상기 실시예 2, 비교예 2 및 비교예 3에서 제조한 유기게르마늄이 흡착된 활성탄 15 g을 필터에 충진 후에 각각의 증류수 1 ℓ, 20 ℓ 및 1000 ℓ를 필터에 통과시켰을 때 유기게르마늄의 용출량을 측정하였다.After filling the filter with 15 g of the activated germanium-adsorbed activated carbon prepared in Example 2, Comparative Example 2 and Comparative Example 3, the amount of organic germanium elution was passed when 1 L, 20 L and 1000 L of each distilled water were passed through the filter. Measured.
상기 실험예 2에 따른 유기게르마늄 용출량을 이하의 표 2에 나타낸다.The organic germanium elution amount according to Experimental Example 2 is shown in Table 2 below.
상기 표 2에 나타낸 바와 같이 본 발명에 따른 실시예 2에서 유기게르마늄이 흡착된 다공성 흡착제를 코팅하는 코팅제로써 에틸셀룰로오스 7 g을 사용한 비교예 2는 증류수 1 ℓ를 통과시켰을 때 유기게르마늄 용출량이 1229 ppb로 유기게르마늄이 다량 용출되고, 1000ℓ를 통과시켰을 때 유기게르마늄의 용출량이 급격히 감소되었다. 이에 반해 에틸셀룰로오스 35 g을 사용한 비교예 3은 증류수 1ℓ를 통과시켰을 때 유기게르마늄 용출량이 234 ppb로 적게 용출되었으나 증류수 1000 ℓ를 통과시켰을 때 유기게르마늄 용출량이 1.80 ppb로 매우 적게 용출되는 것을 나타내어 코팅층이 두꺼우면 유기게르마늄이 지속적으로 용출되기 어려운 것을 유추할 수 있었다.As shown in Table 2, Comparative Example 2 using 7 g of ethyl cellulose as a coating agent coating a porous adsorbent on which organic germanium is adsorbed in Example 2 according to the present invention has an organic germanium elution of 1229 ppb when 1 liter of distilled water is passed. As a result, large amounts of organic germanium were eluted, and the amount of organic germanium eluted was drastically reduced when passed through 1000 L. On the contrary, Comparative Example 3 using 35 g of ethyl cellulose showed less elution of organic germanium at 234 ppb when 1 liter of distilled water was passed, but very small amount of organic germanium elution at 1.80 ppb when 1000 liter of distilled water was passed. If thick, it could be inferred that organic germanium is difficult to continuously elute.
따라서 본원발명에 따른 실시예 2의 유기게르마늄 용출량은 증류수 1000 ℓ를 통과시켰을 경우에도 비교예 2 및 3에 비해 유기게르마늄의 용출량이 지속적으로 유지되는 것을 나타내었다.Therefore, the organic germanium elution amount of Example 2 according to the present invention showed that the elution amount of the organic germanium is continuously maintained in comparison with Comparative Examples 2 and 3 even when passing 1000 L of distilled water.
본 발명자는 유기게르마늄을 흡착시킨 다공성 흡착제를 에틸셀룰로오스로 코팅한 필터를 사용한 가정용 정수기를 통과한 물이 우리나라에서 규정하고 있는 먹는 물의 수질검사에 적합여부를 판정하기 위해서 현재 우리나라에서 규정하고 각각의 일반세균(Total colony counts), 총대장균군(Total Coliforms), 분원성연쇄상구군(Fercal Streptococcus), 농녹균(Pseudomonas aeruginosa), 살모넬라(Salmonella Typhi), 쉬겔라(Shigella), 아황산환원염기성 포자형성균(Spore Forming Sulfite Reducing Anaerobes) 및 여시니아균(Yersinia)의 균수에 적합한지 이하의 실험예 1 내지 10을 통해서 입증하고자 한다.The present inventors are currently defined in Korea to determine whether the water passed through the domestic water purifier using a filter coated with an ethyl cellulose porous organic adsorbent adsorbed organic germanium is suitable for the water quality test of drinking water prescribed in Korea and Total colony counts, Total Coliforms, Fercal Streptococcus , Pseudomonas aeruginosa ), Salmonella Typhi , Shigella , Spore Forming Sulfite Reducing Anaerobes and Yersinia I would like to.
실험예Experimental Example 1: 일반세균(Total colony counts) 1: Total colony counts
1. 표준 한천배지1. Standard Agar Badge
물 1.0 ℓ에 트립톤 5.0 g, 효모엑스 2.5 g, 포도당 1.0 g 및 한천 15.0 g을 넣고 가열하여 녹인 후, pH가 7.0 ± 0.2가 되도록 조정한 다음 10~12 ㎖씩 시험관에 나누어 넣고 121 ℃에서 15분간 고압증기멸균하였다.To 1.0 ℓ of water, tryptone 5.0 g, yeast extract 2.5 g, glucose 1.0 g and agar 15.0 g were dissolved by heating. After adjusting the pH to 7.0 ± 0.2, divided into 10 ~ 12 ㎖ in a test tube at 121 ℃ High pressure steam sterilization for 15 minutes.
2. 인산완충희석액2. Phosphate buffer diluent
인산2수소칼륨 34 g을 500 ㎖의 물에 녹인 후 수산화나트륨용액(0.5N)으로 실온에서 pH를 7.2 ± 0.2로 조정한 후 물을 넣어 1ℓ가 되도록 만들어 이를 보존용원액으로 한다. 이 보존용원액 1.25 ㎖와 염화마그네슘(6수염) 81.1 g을 물 1 ℓ에 녹여 만든 염화마그네슘용액 5.0 ㎖를 넣은 다음 물로 1 ℓ가 되도록 하여 인산완충희석액을 만든다. 인산완충희석액은 99 ± 2 ㎖ 되도록 나선식 마개가 있는 희석병이나 시험관에 나누어 121 ℃에서 15분간 고압증기멸균하였다.Dissolve 34 g of potassium dihydrogen phosphate in 500 ml of water, adjust the pH to 7.2 ± 0.2 at room temperature with sodium hydroxide solution (0.5N), add water to make 1 liter, and use it as a stock solution for preservation. 1.25 ml of this stock solution and 81.1 g of magnesium chloride (hexahydrate) are dissolved in 1 L of water, and 5.0 ml of magnesium chloride solution is added to make 1 L with water to form a phosphate buffer diluent. Phosphate-buffered diluent was divided into dilution bottles or test tubes with a screw cap so that 99 ± 2 ml was autoclaved at 121 ° C. for 15 minutes.
3. 기구 및 장치3. Appliances and Devices
피펫 또는 자동 피펫 (용량 1~5 ㎖의 메스피펫이나 자동피펫(플라스틱팁 포함)으로 멸균된 것을 사용한다), 페트리접시 (지름 약 9 ㎝, 높이 약 1.5 ㎝의 유리제품(65 ㎠) 또는 1회용 플라스틱 제품(57 ㎠)으로 멸균된 것을 사용한다), 항온 수욕조(수온을 45 ± 0.5 ℃로 유지할 수 있는 것을 사용한다), 배양기 (배양온도를 21 ± 1.0 ℃로 유지할 수 있는 것을 사용한다), 집락계수기 (확대경과 조명장치가 부착되어 있고 집락을 계수하기 좋도록 페트리접시를 놓는 판에 1 ㎠로 구획이 그려진 것을 사용한다)Pipettes or automatic pipettes (with sterilized volumetric pipettes of 1 to 5 ml or with automatic pipettes (including plastic tips)), petri dishes (approximately 9 cm in diameter, 1.5 cm in height) or 1 Sterilized with disposable plastic products (57 cm 2)), constant temperature water bath (uses water temperature maintained at 45 ± 0.5 ° C), incubator (uses culture temperature maintained at 21 ± 1.0 ° C) ), Colony counter (use a magnifier and lighting device attached and partitioned with 1 cm 2 on the plate on which the petri dish is placed to facilitate the colony counting)
4. 시험방법4. Test Method
가. 검액의 조제와 시험end. Preparation and Testing
본 발명에 따른 필터를 사용한 정수기에서 정수한 물(이하 "검수"라 약칭함)을 4 ℃ 이하에서 얼지 않도록 냉장 보관하여야 하며 이 경우라도 12시간 이내에 분석을 시작하여야 한다. 12시간 이내에 분석을 시작하지 못할 경우에는 정수의 일반세균은 실험하지 아니한다.Water purified in a water purifier using a filter according to the present invention (hereinafter abbreviated as "inspection") should be refrigerated so as not to freeze below 4 ° C, even in this case, analysis should be started within 12 hours. If the analysis cannot be started within 12 hours, the normal bacteria of the purified water are not tested.
나. 검수를 인산완충희석액 또는 펩톤희석액을 사용하여 10단계 희석법으로 적당한 농도(1 ㎖당 세균수가 30~300개로 추정될 수 있는 농도)로 희석하고 각 단계 희석액 1 ㎖씩을 멸균된 각 페트리접시 2매 이상에 넣는다.I. Dilute the test to 10% dilution using phosphate buffered dilution or peptone dilution to an appropriate concentration (concentration of 30 to 300 bacteria per 1 ml) and add 1 ml of each dilution to at least 2 sterile petri dishes. Put it in.
다. 미리 멸균시켜 45 ± 0.5 ℃로 유지시킨 R2A배지 10~12 ㎖씩을 각각 검액이 들어 있는 페트리접시에 무균적으로 나누어 넣고 배지와 검수가 잘 혼합되도록 좌우로 회전한다.All. 10 to 12 ml of R2A medium sterilized in advance and maintained at 45 ± 0.5 ° C are aseptically divided into petri dishes containing the sample solution, and then rotated side to side to mix well with the medium.
라. 배지가 응고되면 21 ± 1.0 ℃에서 72 ± 3시간 배양하여 형성된 집락의 수를 계산한다.la. Once the medium has solidified, count the number of colonies formed by incubating 72 ± 3 hours at 21 ± 1.0 ° C.
마. 대조군시험으로 멸균된 희석액을 상기 방법과 동일하게 실험하여 대조군으로 한다.hemp. Dilute solution sterilized by the control test was carried out in the same manner as the above method as a control.
바. 검수의 희석 조작부터 평판용 배지를 페트리접시에 나누어 넣을 때까지의 조작시간은 20분을 초과하지 않아야 한다.bar. The operation time from dilution of the inspection to dividing the plate medium into the petri dish shall not exceed 20 minutes.
5. 세균집락수의 계산5. Calculation of bacterial colonies
가. 배양 후 즉시 집락계수기를 이용하여 확산집락이 없고 1평판 당 30~300개의 집락을 형성한 평판을 택하여 집락을 계수하는 것을 원칙으로 한다.end. In principle, the colony should be counted by using a colony counter immediately after incubation and having no colony and having 30 to 300 colonies per 1 flat plate.
나. 평판마다 300개 이상의 집락이 형성되었을 때에는 가장 대표적인 평판을 택하여 밀집평판측정법에 따라 집락수를 계산한다. 계산방법은 안지름 9 ㎝의 유리 페트리접시를 사용한 경우에는 1 ㎠내의 집락수를 13군데에서 계수하여 평균한 집락수에 65를 곱하고, 1회용 플라스틱 페트리접시를 사용한 경우에는 57을 곱한다.I. When more than 300 colonies are formed per plate, the most representative plate is selected and the number of colonies is calculated according to the dense plate measurement method. In the calculation method, when a glass petri dish with an inner diameter of 9 cm is used, the colony number averaged by counting 13 colonies within 1 cm 2 is multiplied by 65, and when a disposable plastic petri dish is used, it is multiplied by 57.
다. 평판마다 30개 이하의 집락이 형성되었을 때에는 원액을 접종한 평판의 집락을 계수하여 평균하며 기재는 반드시 ㎖중 몇 CFU(Colony Forming Unit)라고 한다.All. When less than 30 colonies are formed per plate, the colonies of the plate inoculated with the stock solution are counted and averaged, and the substrate is necessarily called CFU (Colony Forming Unit) in ml.
라. 30~300개의 집락을 가지는 평판이 없고 300개 이상의 집락을 가지는 평판이 1개 이상 존재하는 경우 300개에 가장 가까운 평판의 집락을 계수한다.la. If there are no plates with 30 to 300 colonies and more than one plate with 300 or more colonies is present, the colonies of the nearest 300 plates are counted.
마. 계산 방법은 해당 희석배수에 사용된 각 평판 내의 집락수를 측정하여 합한 다음 사용한 평판수로 나누어 평판당 평균집락수를 구하여 여기에 해당 희석배수를 곱한 수치를 저온일반세균수로 하며, ㎖중 몇 CFU라고 기재한다. 모든 계수 과정에서는 저온일반세균수가 100이상일 때는 높은 단위 숫자로부터 3단계 이하는 사사 오입하여 유효숫자를 2단계로 끊어 그 이하를 0으로 한 수치를 1 ㎖중의 일반세균수로 하고 일반세균수가 100미만일 때에는 소숫점이하는 버린 수치를 1 ㎖ 중의 일반세균수로 기재한다.hemp. The calculation method is to measure the number of colonies in each plate used for the corresponding dilution factor, add them, divide them by the number of plates used, calculate the average number of colonies per plate, and multiply the corresponding dilution factor by the low temperature general bacterial count. Described as CFU. In all counting procedures, when the low temperature general bacterial count is 100 or more, three or less steps are rounded off from the high unit number, and the effective number is cut in two steps, and the number less than 0 is the general bacterial count in 1 ml. In this case, the number discarded by the decimal point shall be written as the general number of bacteria in 1 ml.
실험예Experimental Example 2 : 총대장균군(Total 2: total coliform group (Total ColiformsColiforms ))
1. 2배농후 젖당 배지(추정시험용 배지)1.Lactose lactose medium after double concentration (medium for estimation test)
물 1 ℓ에 소고기엑스 6.0 g, 펩톤10.0 g 및 젖당 10.0 g을 넣고 가열하여 녹인 후, pH가 6.9 ± 0.2가 되도록 조정한 다음 다람 시험관이 들어있는 중시험관에 10 ㎖씩 나누어 넣고 121 ℃에서 15분간 고압증기 멸균하였다.Add 6.0 g of beef extract, 10.0 g of peptone, and 10.0 g of lactose to 1 l of water, dissolve by heating, adjust the pH to 6.9 ± 0.2, and divide 10 ml into a heavy test tube containing a Dharam test tube. Autoclaved for minutes.
2. BGLB(Brilliant Green Lactose Bile)배지(확정시험용 배지)2.BGLB (Brilliant Green Lactose Bile) medium (determination test medium)
물 1 ℓ에 소담즙 20.0 g, 펩톤 10.0 g, 젖당 10.0 g 및 브릴리안트그린 0.0133 g을 넣고 가열하여 녹인 후, pH가 7.2 ± 0.2가 되도록 조정한 다음 다람시험관이 들어있는 소시험관에 10㎖씩 나누어 넣고 121 ℃에서 15분간 고압증기 멸균한다.To 1 liter of water, add 20.0 g of bovine bile, 10.0 g of peptone, 10.0 g of lactose, and 0.0133 g of brilliant green, dissolve by heating, adjust the pH to 7.2 ± 0.2, and add 10 ml to a small test tube containing a Dharam test tube. Dividing and autoclaving at 121 ℃ for 15 minutes.
3. 기구 및 장치3. Appliances and Devices
피펫 (용량 5~10 ㎖의 메스피펫이나 자동피펫으로서 멸균된 것을 사용한다), 시험관 (소시험관(내경 16 ㎜, 높이 150 ㎜) 및 중시험관(내경 18 ㎜, 높이 180㎜)의 2종류 시험관으로 솜(플라스틱이나 금속뚜껑도 사용가능)으로 마개를 할 수 있고 고압증기 멸균할 수 있어야 한다), 다람시험관 (다람시험관(내경 6 ㎜, 높이 30 ㎜)으로 고압증기멸균할 수 있어야 한다), 백금이 (고리의 안지름이 약 3 ㎜인 백금이를 사용한다) 및 배양기 (배양온도를 35 ± 0.5 ℃로 유지할 수 있는 것을 사용한다.)Pipette (use sterilized pipettes or auto pipettes with a capacity of 5-10 ml), test tubes (small test tubes (16 mm in diameter, 150 mm in height)) and heavy test tubes (18 mm in diameter, 180 mm in height) It can be plugged with cotton (plastic or metal lid can also be used) and can be sterilized under high pressure steam), Dharam test tube (with internal diameter 6 mm, height 30 mm), and can be autoclaved with high pressure. Platinum (use platinum rings with a ring diameter of about 3 mm) and incubators (use one that can maintain the culture temperature at 35 ± 0.5 ° C.)
4. 시험4. Test
⑴ 추정시험⑴ Estimation test
㈎ 먹는물에 대한 추정시험은 2배 농후의 젖당배지 또는 라우릴트립토스배지가 10 ㎖씩 들어 있는 중시험관(다람시험관이 들어있는 시험관) 10개에 검수 10 ㎖씩을 접종하여 35 ± 0.5 ℃에서 24 ± 2시간 배양한 다음 가스가 발생하면 추정시험 양성으로 판정하고 확정시험을 실시한다 (3배 농후 배지 10 ㎖가 들어 있는 대시험관 5개에 검수 20 ㎖를 접종할 수도 있다).추정 The preliminary test for drinking water was inoculated with 10 ml of the test in 10 heavy test tubes (10 test tubes containing Dharam test tube) containing 10 ml of double lactose medium or lauryl trypsin medium at 35 ± 0.5 ℃. After 24 ± 2 hours of incubation, if the gas is generated, the test is considered positive and the definitive test is carried out (20 ml of the test may be inoculated into 5 large test tubes containing 10 ml of 3-fold enriched medium).
㈏ 배양 24 ± 2시간 경과 후 어느 시험관에서도 가스가 발생하지 않으면 동일한 조건으로 48 ± 3시간까지 배양하여 여전히 가스발생이 없을 때에는 추정시험 음성으로 판정하고, 하나 이상의 시험관에서 가스발생이 관찰되었을 때에는 확정시험을 실시한다.가스 If no gas is generated in any of the test tubes after 24 ± 2 hours of incubation, incubate up to 48 ± 3 hours under the same conditions. If there is still no gas, the test is judged negative. Conduct the test.
⑵ 확정시험⑵ Confirmation test
㈎ 추정시험에서 가스가 발생하였을 때에는 가스가 발생한 모든 시험관으로부터 배양액을 1백금이씩 취하여 BGLB배지가 10 ㎖씩 들어있는 시험관(다람시험관이 들어있는 시험관)에 각각 접종시켜 35 ± 0.5 ℃에서 48 ± 3시간 배양한다.가스 When gas is generated in the estimation test, 1 ml of platinum solution is taken from all the test tubes in which the gas is generated, and each plate is inoculated into a test tube containing 10 ml of BGLB medium (a test tube containing a squid test tube), and then 48 ± 35 ± 0.5 ° C. Incubate for 3 hours.
㈏ 이때 가스가 발생하지 않으면 총대장균군 확정시험 음성으로 판정하고 가스발생이 관찰되었을 때에는 총대장균군 양성으로 판정한다.㈏ At this time, if no gas is generated, it is determined to be negative for total coliform group confirmation test. If gas is observed, it is determined to be positive for total coliform group.
실험예Experimental Example 3: 3: 분원성연쇄상구균Fecal Streptococcus (( Fecal StreptococcusFecal streptococcus ))
1. 3배 농후 아자이드 포도당배지 (추정시험용배지)1. Triple Concentration Azide Glucose Medium (Estimate Test Medium)
물 1 ℓ에 소고기 엑스 13.5 g, 트립톤 45.0 g, 포도당 22.5 g, 염화나트륨 22.5 g 및 아자이드나트륨 0.6 g을 넣고 가열 용해한 후 실온에서 pH가 7.2 ± 0.2가 되도록 조정한 다음 대시험관에 25 ㎖씩 나누어 넣고 121 ℃에서 15분간 고압증기멸균한다.Add 13.5 g of beef, 15.0 g of tryptone, 45.0 g of glucose, 22.5 g of sodium chloride, 22.5 g of sodium chloride, and 0.6 g of sodium azide, and dissolve the mixture at a temperature of 7.2 ± 0.2 at room temperature. Dividing and autoclaving at 121 ℃ for 15 minutes.
2. 파이자 장구균선택 한천배지 (확정시험용배지)2. Pieza enterococci agar medium (determination test medium)
물 1 ℓ에 펩톤 C 17.0 g, 펜톤 B 3.0 g, 효모엑스 5.0 g, 세균학적 담즙분말 10.0 g, 염화나트륨 5.0 g, 구연산나트륨 1.0 g, 에스쿠린 1.0 g, 구연산암모늄 제2철 0.5 g, 아자이드 나트륨 0.25 g 및 한천 15.0 g을 넣고 가열 용해하여 실온에서 pH가 7.1 ± 0.2가 되도록 조정한 다음 121 ℃에서 15분간 고압증기멸균한 후 50~55 ℃까지 식힌 후 멸균된 페트리 접시에 약 15 ㎖ 씩 무균적으로 나누어 넣고 굳혀서 평판배지를 조제한다.1 L of water, 17.0 g of peptone C, 3.0 g of Fenton B, 5.0 g of yeast extract, 10.0 g of bacteriological bile powder, 5.0 g of sodium chloride, 1.0 g of sodium citrate, 1.0 g of escrine, 0.5 g of ferric ammonium citrate, azide Add 0.25 g of sodium and 15.0 g of agar, heat dissolve, adjust pH to 7.1 ± 0.2 at room temperature, autoclave at 121 ° C for 15 minutes, cool to 50-55 ° C, and add about 15 ml to sterile petri dish. Aseptically divide and solidify to prepare a flat medium.
3. 기구 및 장치3. Appliances and Devices
기구 및 장치는 대장균군시험과 동일한 것을 사용한다.Apparatus and equipment shall be the same as those for E. coli testing.
4. 시험4. Test
⑴ 추정시험⑴ Estimation test
검수 50 ㎖씩을 5개의 3배 농후 아자이드 포도당배지가 25 ㎖씩 들어있는 대시험관에 접종시킨 다음 35 ± 0.5 ℃에서 24 ± 2시간 배양하여 혼탁이 관찰되지 않을 경우 48 ± 3시간까지 연장하여 배양한다. 배양 후 배지의 혼탁 유무를 관찰한다. 어느 시험관에서도 배지가 흐려지지 않는 것은 추정시험 음성으로 하고, 하나 이상의 시험관에서 흐린 것을 확인할 수 있는 것은 세균의 증식, 시료의 혼입에 의한 어느 것이든 관계없이 추정시험 양성으로 판정하고, 다음의 확정시험을 실시한다.Inoculate 50 ml of the test into a large test tube containing 25 ml of five 3-fold enriched azide glucose medium and incubate at 35 ± 0.5 ° C for 24 ± 2 hours. If no clouding is observed, extend the culture to 48 ± 3 hours. do. After culture, observe the media turbidity. In any test tube, the medium does not become cloudy, the estimated test is negative, and in one or more test tubes, it is determined that the estimated test is positive regardless of bacterial growth or mixing of the sample. Is carried out.
⑵ 확정시험⑵ Confirmation test
추정시험에서 흐림을 확인한 모든 시험관으로부터 1백금이씩을 취하여 각각 파이자 장구균 선택 한천배지에 획선이식하여 35 ± 0.5 ℃에서 24 ± 2시간 배양한다. 배양 후 집락을 관찰하는데 갈색의 테두리가 있는 흑갈색의 집락이 형성되었을 경우 확정시험 양성 즉 분원성 연쇄상구균 양성으로 판정하고 전형적인 집락이 관찰되지 않을 경우 확정시험 음성으로 판정한다.Take 1 platinum each from all the test tubes that have confirmed cloudy in the presumptive test, and stir each of the P. aureus selective agar medium and incubate for 24 ± 2 hours at 35 ± 0.5 ℃. After culturing, colonies were observed to have a brownish brown colony with a brown rim, which is positive for positive test, i.e., positive for streptococcus, and negative for positive test if no typical colonies were observed.
실험예Experimental Example 4 : 농녹균( 4: Pseudomonas aeruginosa ( pseudomonaspseudomonas aeruginosaaeruginosa ))
1. 3배 농후 아스파라진배지 (추정시험용배지)1.3 times rich asparagine medium (estimated test medium)
물 1 ℓ에 아스파라진 DL 9.0 g, 인산 1수소칼륨 3.0 g 및 황산마그네슘(7수염) 1.5 g을 넣고 가열 용해하여 실온에서 pH가 7.0 ± 0.2가 되도록 조정한 다음 대시험관에 25 ㎖씩 나누어 넣고 121 ℃에서 15분간 고압 증기 멸균한다.Into 1 liter of water, 9.0 g of asparagine DL, 3.0 g of potassium monohydrogen phosphate, and 1.5 g of magnesium sulfate (7) must be dissolved by heating to adjust the pH to 7.0 ± 0.2 at room temperature. Sterilize by autoclaving at 121 ° C. for 15 minutes.
2. 아세트아미드배지 (확정시험용배지)2. Acetamide medium (determination test medium)
물 1 ℓ에 아세트아미드 10.0 g, 염화나트륨 5.0 g, 인산 1수소칼륨 1.39 g, 인산 2수소칼륨 0.73 g, 황산마그네슘(7수염) 0.5 g 및 페놀레드 0.012 g을 넣고 가열 용해하여 실온에서 pH가 7.0 ± 0.2가 되도록 조정한 다음 소시험관에 10 ㎖씩 나누어 넣고 121 ℃에서 15분간 고압 증기 멸균한다.To 1 liter of water, 10.0 g of acetamide, 5.0 g of sodium chloride, 1.39 g of potassium dihydrogen phosphate, 0.73 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate (hexahydrate), and 0.012 g of phenol red were added and dissolved in heat. After adjusting to ± 0.2, divide 10 ml into small test tubes and autoclave at 121 ℃ for 15 minutes.
3. 트립틱 소이 증균배지3. Tryptic soy enrichment medium
물 1ℓ에 카제인 췌장액 분해산물 17.0 g, 대두분말 파파인 분해산물 3.0 g, 염화나트륨 5.0 g, 인산 2칼륨 2.5 g 및 포도당 2.5 g을 넣고 완전히 녹인다. 121 ℃에서 15분간 고압증기멸균한 후 멸균된 페트리 접시에 약 15 ㎖씩 무균적으로 분주한 후 굳힌다.To 1 liter of water, add 17.0 g of casein pancreatic lysate, 3.0 g of soybean powder papain lysate, 5.0 g of sodium chloride, 2.5 g of dipotassium phosphate, and 2.5 g of glucose. Sterilize by autoclaving at 121 ° C. for 15 minutes and aseptically dispense about 15 ml into a sterile Petri dish and harden.
4. 기구 및 장치4. Appliances and Devices
대장균군시험과 동일한 것을 사용하며, 이밖에도 자외선 램프(장파장 365 nm조사), 자외선 방호안경이 필요하다.The same as the coliform bacterium test is used. In addition, an ultraviolet lamp (long wavelength 365 nm irradiation) and ultraviolet protective glasses are required.
5. 시험 방법5. Test method
⑴ 추정시험⑴ Estimation test
검수 50 ㎖씩을 3배 농후 아스파라진배지가 25 ㎖씩 들어 있는 시험관 5개에 각각 접종한다. 검수를 접종한 아스파라진배지는 35 ± 0.5 ℃에서 24 ± 2시간 배양후 암실에서 자외선 방호안경을 끼고, 자외선램프로 장파장의 자외선(365 nm부근)을 조사해서 녹색을 띠는 형광의 유무를 관찰하고, 형광이 발생한 경우 추정시험 양성으로 한다. 다만, 명확한 형광이 확인되지 않는 경우는 배양을 더 지속해서 48 ± 3시간까지 관찰한다. 이때 시험관에서 녹색 형광이 확인되지 않는 것은 녹농균 음성으로 인정한다. 시험관에서 녹색 형광이 인지되는 경우는 추정시험 양성으로서, 녹농균의 존재 여부를 확인하기 위해 다음의 확정시험을 계속해서 실시한다.50 ml of the test is inoculated in 5 test tubes containing 3 ml of asparazine medium and 3 ml of each. Asparazine medium inoculated with the test was incubated at 35 ± 0.5 ℃ for 24 ± 2 hours, and then put on UV protection glasses in the dark room and irradiated with long wavelength ultraviolet rays (near 365 nm) with an ultraviolet lamp to observe the presence of green fluorescence. If fluorescence occurs, the test is positive. However, if no clear fluorescence is found, continue incubation for up to 48 ± 3 hours. At this time, green fluorescence is not confirmed in the test tube. If green fluorescence is detected in the test tube, the test is positive, and the following definite test is continued to confirm the presence of Pseudomonas aeruginosa.
⑵ 확정시험⑵ Confirmation test
추정시험에서 녹색을 띠는 형광을 확인한 모든 시험관에서 바로 백금이를 이용하여 양성 배지의 상층부에서 3회(약 0.1 ㎖) 취하여 각각의 아세트아미드배지의 사면에 골고루 이식하여 35 ± 0.5 ℃로 24~36시간 배양한다. 배양 후 배지에 현저한 색깔 변화가 없는 경우는 녹농균 음성으로 한다. 그러나, 1개의 시험관이라도 적자색을 나타내는 것은 확정시험 양성으로 판정한다.In all test tubes where green fluorescence was confirmed in the estimation test, three times (approximately 0.1 ml) were taken from the upper layer of the positive medium using platinum immediately and evenly transplanted to the slope of each acetamide medium. Incubate for 36 hours. If there is no significant color change in the medium after incubation, it is negative for Pseudomonas aeruginosa. However, even one test tube exhibiting reddish purple color is determined as positive test positive.
⑶ 확인시험⑶ Confirmation test
확정 양성집락을 순수분리한 후 트립틱 소이 증균배지 혹은 유사배지에서 41.5 ± 0.5 ℃로 48시간 배양하여 성장여부를 관찰한다. 성장하지 않는 집락은 녹농균 음성으로 판정하고 성장한 집락은 그람음성간균임을 그람염색 후 현미경 관찰로 확인한 후 API 20NE와 같은 상용화된 동정시험 Kit를 사용하여 동정하거나 아래에 명시된 생화학적 특징을 검사한다. 생화학적 확인시험에서 녹농균으로 동정되면 녹농균 양성으로 판정한다.After pure separation of definite positive colonies, incubate at 41.5 ± 0.5 ℃ for 48 hours in tryptic soy enrichment medium or similar medium to observe growth. Non-growing colonies are determined to be Pseudomonas aeruginosa (N. aeruginosa) negative and grown colonies are Gram-negative bacterium, followed by gram staining, followed by microscopic observation. If it is identified as Pseudomonas aeruginosa in a biochemical identification test, it is determined to be Pseudomonas aeruginosa positive.
실험예 5: 아황산환원혐기성포자형성균 (Spore Forming Sulfite Reducing Anaerobes ) Experimental Example 5: Spore Forming Sulfite Reducing Anaerobes
1. 2배 농후 DRCM배지1.2x rich DRCM medium
물 1 ℓ에 효모엑스 6.0 g, 소고기엑스 20.0 g, 트립트케이스 20.0 g, 포도당 10.0 g, 염화나트륨 10.0 g, 가용성 전분 2.0 g, 시스테인(염산염) 1.0 g, 초산 나트륨 6.0 g 및 한천 0.5 g을 넣고 가열 용해하여 실온에서 pH가 6.8 ± 0.2가 되도록 조정한 다음 나선식 마개가 있는 중시험관에 10 ㎖ 씩 분주해서 121 ℃에서 15분간 고압 증기멸균한다. 실험 시에는 각각의 시험관에 여과 멸균한 4 % 구연산 제2철과 7 % 아황산나트륨을 같은량 혼합한 액을 각각 0.4 ㎖ 넣는다.To 1 liter of water, add 6.0 g of yeast extract, 20.0 g of beef extract, 20.0 g of trycase, 20.0 g of glucose, 10.0 g of sodium chloride, 2.0 g of soluble starch, 1.0 g of cysteine (hydrochloride), 6.0 g of sodium acetate, and 0.5 g of agar. Heat dissolve to adjust the pH to 6.8 ± 0.2 at room temperature. Dispense 10 ml into a heavy test tube with a spiral plug and autoclave at 121 ° C for 15 minutes. In the test, 0.4 ml of a mixture of 4% ferric citrate and 7% sodium sulfite in equal amounts was added to each test tube.
2. 기구 및 장치2. Appliances and Devices
기구 및 장치는 대장균군 시험과 동일한 것을 사용하며 추가로 항온수욕조(수온을 75 ± 1.0 ℃로 유지할 수 있는 것을 사용한다.)가 필요하다.The apparatus and equipment are the same as those for the E. coli group test and additionally require a constant temperature bath (which can maintain a water temperature of 75 ± 1.0 ° C).
3. 시험 방법3. Test method
⑴ 아포형성시험⑴ Apoptosis test
검수 10 ㎖씩을 5개의 나선식 마개가 있는 시험관에 무균적으로 넣은 후 수온을 75 ± 1.0 ℃로 유지한 항온수욕조에 10분간 정치한다. 이때 아포를 형성하지 못하는 균은 모두 사멸하고 아포를 형성할 수 있는 세균만 살게 된다.Each 10 ml of the test is aseptically placed in a test tube with five spiral plugs, and then allowed to stand for 10 minutes in a constant temperature bath maintained at 75 ± 1.0 ° C. At this time, all the bacteria that can not form apo will die and live only the bacteria that can form apo.
⑵ 배양 및 아황산 환원력시험⑵ Culture and sulfite reduction test
75 ± 1.0 ℃의 항온수욕조에서 10분간 정치시킨 5개의 나선식 마개가 있는 시험관을 즉시 20 ℃ 전후로 냉각한 다음 미리 준비한 2배 농후 DRCM 배지를 각각 10㎖씩 넣는다. 그리고 기포가 생기지 않도록 검수와 배지를 혼합한 후 미리 멸균한 액체파라핀을 2~3 ㎖씩 무균적으로 배지 표면에 넣고 마개를 단단히 닫는다. 그리고 35 ± 0.5 ℃로 48 ± 3시간 배양한다. 배양 후 배지가 검게 변하게 되면 아황산이 환원된 것으로 아황산환원 혐기성포자 형성균 양성으로 판정하며 5개의 시험관 중 1개라도 양성인 경우 검출되었다고 판정한다.After cooling the test tube with five helix plugs, which have been allowed to stand for 10 minutes in a 75 ± 1.0 ° C constant temperature bath, immediately cools to around 20 ° C, and adds 10 ml of each of the two-packed DRCM medium prepared in advance. After mixing the inspection and the medium to prevent bubbles, put the sterilized liquid paraffin into the surface of the medium aseptically every 2-3 ml and close the cap tightly. Incubate at 48 ± 3 hours at 35 ± 0.5 ° C. When the medium turns black after incubation, the sulfurous acid is reduced and is determined to be positive for sulfite-reduced anaerobic spore-forming bacteria, and it is determined that any one of five test tubes is positive.
실험예Experimental Example 6 : 살모넬라( 6: Salmonella ( Salmonella Salmonella TyphiTyphi ))
1. 3배 농후 셀레나이트 액체증균배지1. Triple enriched selenite liquid enrichment medium
1 ℓ의 물에 트립톤 15.0 g, 젖당 12.0 g, 셀레나이트 12.0 g 및 인산나트륨 30.0 g을 녹여 끓이지 않고 60~70 ℃에서 가열용해 한 후 멸균된 대시험관에 25 ㎖씩 무균적으로 넣고 식힌다. 조제된 배지는 2~8 ℃에 보관한다.Dissolve 15.0 g of tryptone, 12.0 g of lactose, 12.0 g of selenite, and 30.0 g of sodium phosphate in 1 L of water, dissolve it at 60∼70 ° C without boiling, and sterilize 25 ml of each in a sterile tube. The prepared medium is stored at 2-8 ℃.
2. 비스무스 아황산염 선택배지 -추정시험용2. Bismuth sulfite selective medium -For estimation test
1ℓ에 펩톤 10.0 g, 소고기 엑스 5.0 g, 포도당 5.0 g, 인산 1수소나트륨 4.0 g, 황산제1철 0.3 g, 비스무스아황산염 8.0 g, 브릴리언트 그린 0.025 g 및 한천 20.0 g을 넣고 가열 용해한 후 50~55 ℃까지 식혀 멸균된 페트리접시에 분주하여 고체화한다. 가열시 끓는 상태가 1~2분 이상 지속되지 않도록 한다. 조제된 배지는 2~8 ℃에서 보관하며 4일 이상 저장하지 않는다.1 l of peptone, 1 g of beef, 5.0 g of beef, 5.0 g of glucose, 4.0 g of sodium hydrogen phosphate, 0.3 g of ferrous sulfate, 8.0 g of bismuth sulfite, 0.025 g of brilliant green and 20.0 g of agar were dissolved and heated to 50-55. Cool to ºC and dispense into a sterile Petri dish to solidify. Do not boil for more than 1 ~ 2 minutes when heated. Prepared medium is stored at 2 ~ 8 ℃ and not stored for more than 4 days.
3. 기구 및 장치는 대장균군 시험에서와 동일한 것을 사용하며 추가로 냉장고 (2~5 ℃), 광학현미경, 가스버너, 알코올램프 및 슬라이드 글라스가 필요하다.3. Apparatus and equipment are the same as those used for E. coli testing and additionally require refrigerator (2 ~ 5 ℃), optical microscope, gas burner, alcohol lamp and slide glass.
4. 시험4. Test
⑴ 추정시험⑴ Estimation test
검수 50 ㎖씩을 3배 농후 셀레나이트 증균배지가 25 ㎖씩 들어있는 시험관 5개에 접종한다. 검수를 접종한 셀레나이트배지는 37 ℃에서 18~24시간 배양한다. 배양시간이 지연되면 대장균 등 다른 균이 자라므로 배양시간을 엄수한다. 백금이를 이용하여 모든 시험관에서 증균배지 배양액을 취하여 비스무스 한천 선택배지에 획선 접종한다. 35 ℃에서 24시간 배양 후 집락을 관찰하고 다시 배양하여 48시간후 재 관찰하여 가장자리에 하얀 테를 두른 검고 반짝이는 집락을 살모넬라 양성으로 추정한다. 전형적인 양성집락이 없고 녹색집락이 생성되었을 경우도 집락을 취하여 확인시험한다.50 ml of the test is inoculated into five test tubes containing 25 ml of three times enriched selenite enrichment medium. Inoculated selenite medium is incubated at 37 ℃ for 18-24 hours. If the incubation time is delayed, other bacteria such as Escherichia coli grow, so strictly observe the incubation time. Using platinum, take the enrichment medium from all the tubes and inoculate the bismuth agar selection medium. Observe colonies after incubation at 35 ° C. for 24 hours, incubate again, and re-observe after 48 hours, presuming that the black and shiny colonies with white rims at the edges are positive for Salmonella. If there is no typical benign colony and a green colony is produced, the colony is also tested for identification.
⑵ 확인시험⑵ Confirmation test
㈎ 생화학적 확인시험: 추정시험에서 양성으로 판정된 집락을 순수 분리하여 선택배지로 옮겨심는다. 그람염색 후 현미경 관찰로 그람 음성, 간균임을 확인하고 API 20E kit를 사용한다. 생화학적 확인시험으로 살모넬라속으로 동정되면 살모넬라균 양성으로 판정한다. 본실험은 양성판정으로 본 실험을 종료하며, 만약 병원성 및 역학에 대한 정보를 얻고자 한다면 더 자세한 종 및 혈청형의 동정을 혈청학적인 시험을 통하여 수행하도록 한다.㈎ Biochemical identification test: Colonies that are positive in the presumptive test are purely separated and transferred to selective medium. After Gram staining, confirm that it is Gram negative and bacillus by microscopic observation and use API 20E kit. If identified as Salmonella by biochemical confirmation, it is determined to be Salmonella positive. The experiment ends with a positive determination and if more informed about pathogenicity and epidemiology, further identification of species and serotypes should be performed through serological tests.
㈏ 혈청학적시험: 생화학적시험에서 살모넬라로 동정된 균들은 살모넬라 O항혈청에 해당하는 A, B, C, D, E그룹 항혈청반응을 보아 그룹을 결정하고 그 그룹에 속하는 단일요소 항혈청으로 시험한다. Vi항혈청에만 응집이 있는 경우는 살모넬라 타이피가 아닌가 확인하고, 균 부유액을 100 ℃에서 끓여 Vi항원을 파괴한 후 O항혈청응집을 보아 그룹을 결정한다. H항혈청으로 편모항원 Phase I, II를 시험한 후 O항원, 편모항원을 종합하여 살모넬라 균종을 확정짓는다. 추가로 여기서 보다 상세하게 살모넬라 종 즉, 종(species)나 항원형(serotype)을 확인하고자 한다면 국립보건원 살모넬라센타에 의뢰하면 최종동정을 확인받도록 한다. 본 발명에서는 살모넬라 균종이 우리나라 먹는물 세균수에 적합한지 여부만을 판정한다.㈏ Serological tests: The bacteria identified as Salmonella in the biochemical test are grouped according to the A, B, C, D and E group antiserum corresponding to Salmonella O antiserum, and tested with single factor antiserum belonging to the group. If there is agglutination only in the Vi antiserum, check whether it is Salmonella typhi, and boil the bacterial suspension at 100 ° C. to destroy the Vi antigen, and then determine the group by viewing the O antiserum aggregation. Test the flagellar antigens Phase I and II with H-serum and determine the Salmonella species by combining the O-antigens and flagellar antigens. In addition, if you want to know more specifically Salmonella species, such as species (species) or antigen (serotype), please refer to the National Institutes of Health Salmonella Center for final identification. In the present invention, it is determined only whether the Salmonella species is suitable for the number of drinking water bacteria in Korea.
실험예Experimental Example 7: 쉬겔라( 7: Shigella ShigellaShigella ))
1. 3배 농후 셀레나이트 액체증균배지는 살모넬라 증균배지와 동일하게 제조한다.1. Triple-fold selenite liquid enrichment medium is prepared in the same manner as Salmonella enrichment medium.
2. XLD(Xylose Lysine Deoxycholate)) 한천 선택배지(추정시험용)2. XLD (Xylose Lysine Deoxycholate) agar selection medium (for estimation test)
1 ℓ의 물에 효모엑스 3.0 g, L-라이신 5.0 g, 자이로즈 3.75 g, 젖당 7.5 g, 자당 7.5 g, 데속시콜린산나트륨 2.5 g, 구연산제2철암모늄 0.8 g, 티오황산나트륨 6.8 g, 염화나트륨 5.0 g, 한천 15.0 g 및 페놀레드 0.08 g을 넣고 완전용해 될 때까지 가열한다. 55~60 ℃까지 식힌 후 멸균된 페트리디쉬에 분주하여 고체화시킨다. 조제된 배지는 2~8 ℃에서 보관한다.1 liter of water, 3.0 g of yeast extract, 5.0 g of L-lysine, 3.75 g of gyros, 7.5 g of lactose, 7.5 g of sucrose, 2.5 g of sodium desoxycholate, 0.8 g of ferric ammonium citrate, 6.8 g of sodium thiosulfate, Add 5.0 g of sodium chloride, 15.0 g of agar and 0.08 g of phenol red and heat until complete dissolution. After cooling to 55 ~ 60 ℃, aliquot into sterile Petri dishes to solidify. The prepared medium is stored at 2-8 ℃.
3. 기구 및 장치는 살모넬라균 실험법에서와 같은 것을 사용한다.3. Apparatus and devices are the same as in Salmonella test.
4. 시험4. Test
⑴ 추정시험 증균과정은 살모넬라와 동일하다. 배양된 증균배지를 백금이로 취하여 XLD한천선택배지에 살모넬라와 같은 방식으로 획선 접종한 후 35 ℃에서 24시간 배양하여 붉은 색 집락이 형성되면 쉬겔라 추정시험의 양성으로 판정한다.The process of enrichment of the putative test is identical to that of Salmonella. The cultured enrichment medium is taken as platinum, and inoculated into XLD agar selective medium in the same manner as Salmonella, followed by incubation at 35 ° C for 24 hours to form a red colony.
⑵ 확인시험⑵ Confirmation test
㈎ 생화학적 확인시험: 살모넬라의 확인시험과 동일하게 시험한다.㈎ Biochemical Confirmation Test: The same test as for Salmonella.
㈏ 혈청학적 시험: 생화학적 확인시험에서 쉬겔라로 동정된 균주의 종을 알고자 할 경우는 쉬겔라 O항혈청 응집반응시험을 한다.㈏ Serological tests: If you want to know the species of strains identified as Shigella in the biochemical identification test, Shigella O antiserum aggregation test.
실험예Experimental Example 8 : 여시니아( 8: Yexinia ( YersiniaYersinia ))
1. CIN 한천 배지1. CIN Agar Badge
여시니아선택 한천기본배지의 구성성분은 효모엑스 2 g, 펩톤 17 g, 프로토오스 펩톤 3 g, 만니톨 20 g, 데속시콜린산나트륨 0.5 g, 염소산나트륨 0.5 g, 염화나트륨 1 g, 피르빈산나트륨 2 g, 황산마그네슘(6수염) 10㎎, 한천 13.5 g, 뉴트럴레드 30 ㎎, 크리스탈바이올렛 1 ㎎, 이르가산(irgasan) 4 ㎎ 및 물 1 ℓ로 구성된다.The components of Yexinia selective agar base medium are yeast extract 2g, peptone 17g, protose peptone 3g, mannitol 20g, sodium desoxycholate 0.5g, sodium chlorate 0.5g, sodium chloride 1g, sodium pyribate 2 g, 10 mg of magnesium sulfate (hexahydrate), 13.5 g of agar, 30 mg of neutral red, 1 mg of crystal violet, 4 mg of irgasan, and 1 L of water.
CIN여시니아 항미생물 보충액의 구성성분은 세프설로이딘(cefsulodin) 4 ㎎, 노보바이오신(novobiocin) 2.5 ㎎ 및 물 10 ㎖이다. 여시니아선택 한천기본배지를 121 ℃에서 15분간 고압멸균한 후 45~50 ℃로 식혀 CN여시니아 항미생물보충액을 첨가한다.The constituents of the CIN cycinia antimicrobial supplement are 4 mg of cefsulodin, 2.5 mg of novobiocin and 10 ml of water. Yeastia selective agar base medium is autoclaved at 121 ℃ for 15 minutes, then cooled to 45 ~ 50 ℃ and added CN Yexinia antimicrobial supplement.
2. 메콩키 한천배지2. Mekongki Agar Badge
펩톤17 g, 프로테오스펩톤 3 g, 유당 10 g, 담즙염 No. 3 1.5 g, 염화나트륨 5 g, 한천 13.5 g, 뉴트럴레드 0.03 g, 크리스탈바이올렛 0.001 g 및 물 1 ℓ(최종 pH (25 ℃) 7.1 ± 0.2)17 g of peptone, 3 g of proteos peptone, 10 g of lactose, bile salt No. 3 1.5 g, sodium chloride 5 g, agar 13.5 g, neutral red 0.03 g, crystal violet 0.001 g and 1 L of water (final pH (25 ° C) 7.1 ± 0.2)
3. mMC배지3. mMC medium
위에 기재한 메콩키 한천베이스에 D-쏘르비톨 10 g/ℓ, 이르가산 4.0 ㎎/ℓ 및 노보바이오신 2.5 ㎎/ℓ 첨가한다.10 g / l of D-sorbitol, 4.0 mg / l of irgasan and 2.5 mg / l of Novobiocin are added to the Mekongi agar base described above.
4. 0.288 % 수산화칼륨용액4. 0.288% potassium hydroxide solution
염화나트륨 8.5 g, 수산화칼륨 2.88 g 및 물 1 ℓ를 혼합한다.8.5 g of sodium chloride, 2.88 g of potassium hydroxide and 1 L of water are mixed.
5. 기구 및 장치5. Appliances and Devices
여과막 (공경 0.45 ㎛, 직경 47 ㎜크기의 미생물 분석용 여과막으로서 막의 한면에 격자가 그려진 멸균된 것을 사용한다.), 여과장치 (여과막을 끼워서 여과할 수 있게 하는 장치로 고압증기멸균가능한 것을 사용한다.), 원형흡수패드 (두꺼운 여지나 스폰지 또는 유리섬유여과막으로 직경 47 ㎜크기의 원형패드를 멸균하여 사용한다.), 페트리접시 (지름 약 5.5 ㎝, 높이 약 1.2 ㎝의 소형제품(24 ㎠)으로 멸균된 것을 사용한다.), 핀셋 (끝이 뭉툭하고 넓으며 여과막을 집어 올릴 때 여과막을 물리적으로 손상시키지 않는 형태의 것으로 화염멸균 가능한 것을 사용한다.)Filtration membrane (Use a sterilized filtration membrane with a grid of 0.45 μm in diameter and 47 mm diameter for microbial analysis.), Filtering device (A device for filtering by inserting a filtration membrane is used. .), Circular absorption pad (Use thick pad or sponge or glass fiber filtration membrane to sterilize 47mm diameter circular pad.), Petri dish (approximately 5.5 cm in diameter, 1.2 cm in height, small product (24 ㎠) Tweezers are blunt and wide, and do not physically damage the membrane when picking up the membrane.
6. 시험6. Test
㈎ 추정시험㈎ Estimation test
검수 2 ℓ를 멸균용기를 사용하여 채취한 후 즉시 시험한다. 즉시 시험할 수 없을 때는 냉장보관상태로 운반하여 조속한 시간 내에 시험한다. 채취한 검수 1 ℓ는 멸균된 여과장치를 이용하여 멸균상태의 여과지(membrane filter, 0.22㎛ pore size))를 통과시킨 후 연속하여 0.288 % 수산화칼륨용액 20~30 ㎖를 20초 내에 완전히 여과 시킨 후 이 여과지를 CIN배지에 그대로 올려놓고 25~28 ℃에서 48시간 배양한다. 나머지 1 ℓ는 동일한 과정을 거쳐서 메콩키배지 (혹은 mMC 배지)에서 배양한다. CIN배지에서는 점액성이 없고 중심부가 짙은 적색을 띠는 작은 집락을 메콩키배지 (혹은 mMC)에서는 적색 혹은 점액성 집락은 피하고 작고(직경 1~2 ㎜), 편평한 무색 혹은 옅은 핑크색의 집락을 취하여 뇌심장 융합배지(Brain Heart Infusion Agar)에 옮긴다.2 l of the test is taken immediately using a sterile container and tested immediately. If it cannot be tested immediately, it shall be transported in refrigerated condition and tested within a short time. 1 L of the collected inspection was passed through a sterilized filter (membrane filter, 0.22㎛ pore size) using a sterile filtration device, and then continuously filtered 20-30 ml of 0.288% potassium hydroxide solution within 20 seconds completely. Put this filter paper on the CIN medium and incubate for 48 hours at 25 ~ 28 ℃. The remaining 1 L is incubated in Mekongki medium (or mMC medium) through the same procedure. In CIN medium, small colonies that are not mucus and have a deep red in the center are avoided in Mekongki medium (or mMC), avoiding red or slime colonies (small 1 ~ 2 mm in diameter), and take flat colorless or pale pink colonies. Transfer to Brain Heart Infusion Agar.
㈏ 예비동정시㈏ Preliminary Identification
TSI배지, 요소배지, 운동성 배지에 접종하여 예비실험을 한다. 예비실험에서 여시니아균의 특성을 나타내지 않는 집락은 여시니아균 음성으로 판정하고 여시니아균의 특성을 지닌 분리 집락은 그람염색시 음성임을 확인한 후 생화학적 확인동정시험를 한다. TSI 배지에서 37 ℃, 24시간 배양시 Y. enterocolitica는 자당을 분해하여 밑면과 사면이 황변하고 Y. pseudotuberculosis는 사면은 황변하고 밑면이 황변하지 않고 적색으로 남는다. 가스 및 황화수소는 발생하지 않는다. 요소배지에서는 양성으로 붉은 색을 나타내며, 운동성시험은 두 개의 운동성 배지에 접종하여 각각 37 ℃와 25 ℃에 따로 배양하여 37 ℃에서는 운동성이 없고, 25 ℃에서는 운동성을 나타낸다.Preliminary experiments are done by inoculating TSI medium, urea medium and kinetic medium. In preliminary experiments, colonies that do not exhibit the characteristics of yeast strains are determined as negative yeast strains, and separated colonies with yeast strains are negative when Gram staining is performed. Y. enterocolitica decomposes sucrose and yellows at the bottom and slope, while Y. pseudotuberculosis is yellow at the slope and the bottom is not yellowed. No gas and hydrogen sulfide are generated. In urea medium, it shows a positive red color. The motility test was inoculated in two kinetic media and incubated separately at 37 ° C. and 25 ° C., respectively.
㈐ 확인동정시험㈐ Identification test
생화학동정시험은 API 20E Kit를 사용한다.Biochemical identification uses the API 20E Kit.
상기 실험예 1 내지 8에서 실험한 본 발명에 따른 유기게르마늄이 흡착된 다공성 흡착제를 필터로 사용한 정수기를 통과한 정수기물이 우리나라 먹는물 기준에 적합한지 여부를 이하의 표 3에 나타낸다.Table 3 below shows whether the purified water passing through the water purifier using the organic germanium-adsorbed porous adsorbent according to the present invention tested in Experimental Examples 1 to 8 meets the Korean drinking water standard.
상기 표 3에 나타낸 바와 같이 본 발명에 따른 실시예와 우리나라 기준과 비교해 보았을 때 본 발명에 따른 필터를 통과한 물은 우리나라 기준에 부합하는 것으로 나타났다. 따라서 유기게르마늄이 흡착된 흡착제와 에틸셀룰로오스로 인해서 정수기의 오염도에 영향을 미치지 않는 것을 입증하였다.As shown in Table 3, the water passing through the filter according to the present invention was found to meet the Korean standard when compared with the example according to the present invention and the Korean standard. Therefore, it was proved that organic germanium did not affect the degree of contamination of the water purifier due to the adsorbent and ethyl cellulose.
또한 우리나라 기준은 대장균과 분원성대장균군을 더 포함하지만 본원발명은 총대장균군에서 우리나라 기준에 적합하여 대장균과 분원성대장균군을 실험하지 않았다.In addition, the Korean standard further includes E. coli and F. coli, but the present invention does not test E. coli and F. coli in the total E. coli.
또한 본 발명자는 유기게르마늄이 흡착된 흡착제와 에틸셀룰로오스를 포함하는 필터를 포함하는 정수기를 사용해서 물을 통과시켰을 때 우리나라 먹는물 기준에서 건강상 유해물질로 규정된 납, 불소, 비소, 셀레늄, 수은, 시안, 암모니아성 질소, 6가 크롬, 질산성 질소, 카드뮴 및 붕소의 함량을 측정하였다.In addition, the present inventors lead, fluorine, arsenic, selenium, and mercury, which are defined as health hazards in Korea's drinking water standards when water is passed through an organic germanium adsorbent and a water purifier including a filter containing ethyl cellulose. The contents of, cyanide, ammonia nitrogen, hexavalent chromium, nitrate nitrogen, cadmium and boron were measured.
본 발명의 실시예 2를 사용한 이하의 실험예 9 내지 19의 실험방법은 환경부 고시 제2002-91호(2002.06.21 개정) 먹는물 수질공정 시험방법에 따른다.The experimental method of the following Experimental Examples 9-19 using Example 2 of this invention is based on the test method of the drinking-water quality process of the Ministry of Environment notification 2002-91 (revised on June 21, 2002).
실험예Experimental Example 9: 납 (Pd; lead) 9: Pd; lead
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-1에 따른다.Ministry of Environment Notice 2002-91 To comply with the test method 2-2-2-1 of drinking water quality process.
실험예Experimental Example 10: 불소 (F; Fluoride) 10: Fluoride
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-1-1에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-1-1.
실험예Experimental Example 11: 비소 (As; Arsenic) 11: Arsenic
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-2 에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-2-2.
실험예Experimental Example 12: 셀레늄 (Se; Selenium) 12: selenium (Se; Selenium)
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-3 에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-2-3.
실험예Experimental Example 13: 수은 (Hg; Mercury) 13: mercury (Hg; Mercury)
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-4 에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-2-4.
실험예Experimental Example 14: 시안 ( 14: Cyan ( CNCN ; Cyanide); Cyanide)
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-1-2 에 따른다.According to the notification of the Ministry of Environment, No. 2002-91, Test Method of Drinking Water Quality Process 2-2-1-2.
실험예Experimental Example 15: 15: 암모니아성질소Ammonia nitrogen ( ( NHNH 33 -N, Ammonium-Nitrogen)-N, Ammonium-Nitrogen)
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-1-3 에 따른다.Ministry of Environment Notice 2002-91 To comply with the test method 2-2-1-3 of drinking water quality process.
실험예Experimental Example 16: 6가 크롬 ( 16: hexavalent chromium ( CrCr 66 ++ ; ; HexachromiumHexachromium ))
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-5 에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-2-5.
실험예Experimental Example 17: 질산성 질소 ( 17: nitrate nitrogen ( NONO 33 -N; Nitrate Nitrogen)-N; Nitrate Nitrogen)
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-1-4 에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-1-4.
실험예Experimental Example 18: 카드뮴 (Cd; Cadmium) 18: Cd; Cadmium
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-6 에 따른다.According to the Ministry of Environment Notification No. 2002-91 Test Method of Drinking Water Quality Process 2-2-2-6.
실험예Experimental Example 19: 보론 (B; Boron) 19: Boron
환경부 고시 제2002-91호 먹는물 수질공정 시험방법 2-2-2-12 유도결합 플라즈마-원자방출법에 따른다.Ministry of Environment Notice No. 2002-91 Test method for drinking water quality 2-2-2-12 Inductively coupled plasma-atomic emission method.
상기 실험예 9 내지 19에 대한 실험결과를 우리나라 먹는물 기준에 적합한지 여부를 이하의 표 4에 나타낸다.Table 4 below shows whether or not the test results for Experimental Examples 9 to 19 meet Korean drinking water standards.
상기 표 4에 나타낸 바와 같이 본 발명은 유기게르마늄을 흡착시킨 다공성 흡착제를 코팅하여 필터로 사용하여도 건강상 유해 영향 무기물질에 영향을 미치지 않아 유기게르마늄을 포함한 물을 안전하게 음용할 수 있음을 입증하였다.As shown in Table 4, the present invention has proved that water containing organic germanium can be safely consumed without affecting the health effects of inorganic substances even when the porous sorbent coated with organic germanium is used as a filter. .
본 발명에 대해 상기 실시예를 참고하여 설명하였으나, 이는 예시적인 것에 불과하여, 본 발명에 속하는 기술 분야의 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 타 실시예가 가능하다는 점을 이해할 것이다. 따라서 본 발명의 진정한 기술적 보호범위는 첨부된 특허청구범위의 기술적 사상에 의해 정해져야 할 것이다.Although the present invention has been described with reference to the above embodiments, these are merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. . Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.
상기에서 상세히 설명한 바와 같이, 본 발명에 따르면 다공성 흡착제를 가열하여 기공을 확대하여 확대된 기공에 유기게르마늄을 흡착시키고 유기게르마늄이 흡착된 흡착제를 에틸셀룰로오스로 코팅하여 정수기의 필터에 사용함으로써 정수 시에 지속적으로 유기게르마늄의 용출되어 유기게르마늄이 포함된 물을 일상적으로 음용할 수 있다.As described in detail above, according to the present invention, the porous adsorbent is heated to enlarge pores to adsorb organic germanium to the enlarged pores, and the organic germanium adsorbent is coated with ethyl cellulose to be used for water purifier filters. Organic germanium is continuously eluted, and water containing organic germanium can be routinely drunk.
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KR101775079B1 (en) | 2015-12-21 | 2017-09-05 | 충북대학교 산학협력단 | Catheter cotaining organic germanium and method for preparing the same |
KR102036844B1 (en) * | 2017-04-19 | 2019-10-25 | (주)진행워터웨이 | Method for preparation of organic germamium from granite |
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JPH04235708A (en) * | 1991-01-18 | 1992-08-24 | Kuraray Co Ltd | Filter and filtration device |
KR940001799A (en) * | 1992-07-20 | 1994-02-16 | 최상선 | How to prepare canned garlic tuna preserved texture |
KR20000074036A (en) * | 1999-05-17 | 2000-12-05 | 한명훈 | A method of purification stationery |
KR20040003753A (en) * | 2002-07-04 | 2004-01-13 | 일신산업주식회사 | A septic tank including zeolite and germanium |
JP2006007160A (en) * | 2004-06-29 | 2006-01-12 | Med Group Kk | Filter |
JP2006026516A (en) * | 2004-07-15 | 2006-02-02 | Seratekku Kk | Water purification material and purification system |
JP2006063040A (en) * | 2004-08-30 | 2006-03-09 | Organic Germanium Kk | Method for producing physiologically active water having high permeability |
KR20050024481A (en) * | 2005-01-28 | 2005-03-10 | 이신종 | Depurator prepared using natural mineral powder containing germanium and method for preparing the same by preparing natural mineral powder, preparing quicklime, mixing natural mineral powder and quicklime and aging mixture |
JP2007038125A (en) * | 2005-08-03 | 2007-02-15 | Nihon Technical Development Center Co Ltd | Functional filter for water purification |
KR100706029B1 (en) | 2006-03-21 | 2007-04-12 | 채수길 | The method and ability ceramics filter |
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2007
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Cited By (3)
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KR102027993B1 (en) * | 2018-12-20 | 2019-10-02 | 김태수 | Composite filter of activating natural minerals customized by health function and method for manufacturing the same |
WO2020130302A1 (en) * | 2018-12-20 | 2020-06-25 | 김태수 | Health function-customized natural mineral activating composite filter, and method for producing same |
KR102505738B1 (en) * | 2022-09-29 | 2023-03-03 | 김동기 | manufacturing method of filter material using Aragonite adsorbed with organic Germanium |
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