KR20080062447A - A method for preparing kimchi showing improved quality and advanced preservation using deep ocean water - Google Patents

A method for preparing kimchi showing improved quality and advanced preservation using deep ocean water Download PDF

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KR20080062447A
KR20080062447A KR1020060138279A KR20060138279A KR20080062447A KR 20080062447 A KR20080062447 A KR 20080062447A KR 1020060138279 A KR1020060138279 A KR 1020060138279A KR 20060138279 A KR20060138279 A KR 20060138279A KR 20080062447 A KR20080062447 A KR 20080062447A
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kimchi
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water
deep sea
deep
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KR100911826B1 (en
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조순영
김옥선
전중균
박신호
임미진
김혜경
김광우
김정식
정순범
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정순범
강릉대학교산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/10Preserving with acids; Acid fermentation
    • A23B7/105Leaf vegetables, e.g. sauerkraut
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/157Inorganic compounds

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Abstract

A method of preparing Kimchi with improved quality and keeping quality by soaking Chinese cabbage in deep sea water instead of a salt solution and mixing with seasonings is provided to give organic salts and minerals in large quantities. Chinese cabbage is cut into two or three pieces and soaked in deep sea water with a salinity of 10 to 15% for 4 to 6hr. The salted Chinese cabbage is soaked in 2 to 3 times the weight of water for 5 to 6hr, washed with clean water, dehydrated, mixed with seasonings and aged for 1 to 2 days. The seasonings contain 80 to 90% by weight of vegetables, 1.0 to 2.0% by weight of radishes with a size of 3.5 to 4.5cm, 1.5 to 2.5% by weight of Welsh onions with a size of 3.5 to 4.5cm 0.5 to 1.0% by weight of ginger, 1.0 to 2.0% by weight of garlic, 2.0 to 4.0% by weight of red pepper powder, 0.5 to 1.5% by weight of sugar and 2.0 to 3.0% by weight of salted anchovies.

Description

해양심층수에 의한 품질개선 및 보존성 향상 김치의 제조방법 {A method for preparing kimchi showing improved quality and advanced preservation using deep ocean water}A method for preparing kimchi showing improved quality and advanced preservation using deep ocean water}

도 1은 5℃ 숙성중에서 제조김치들의 염도 변화를 나타낸 도이고,1 is a diagram showing the salinity change of the kimchi prepared during 5 ℃ aging,

도 2는 5℃ 숙성중에서 제조김치들의 pH 변화를 나타낸 도이며, Figure 2 is a diagram showing the pH change of the kimchi prepared during 5 ℃ aging,

도 3은 5℃ 숙성중에서 제조김치들의 산도 변화를 나타낸 도이고,3 is a view showing the acidity change of the kimchi prepared during 5 ℃ aging,

도 4는 5℃ 숙성중에서 제조김치들의 TBA값 변화를 나타낸 도이며,4 is a view showing the change in TBA value of the kimchi prepared during 5 ℃ aging,

도 5는 5℃ 숙성중에서 제조김치들의 생균수 변화를 나타낸 도이고,5 is a diagram showing the viable cell number of the kimchi prepared during 5 ℃ aging,

도 6은 5℃ 숙성중에서 제조김치들의 곰팡이수(Fungi속) 변화를 나타낸 도이며,6 is a view showing the change in the number of fungi ( Fungi genus) of the kimchi produced during 5 ℃ aging,

도 7은 5℃ 숙성중에서 제조김치들의 유산균수(Leuconostoc속) 변화를 나타낸 도이고,Figure 7 is a diagram showing the change in the number of lactic acid bacteria ( Leuconostoc genus) of the kimchi prepared during 5 ℃ aging,

도 8은 5℃ 숙성중에서 제조김치들의 유산균수(Lactobacillus속) 변화를 나타낸 도이며,8 is a view showing the change in the number of lactic acid bacteria ( Lactobacillus genus) of the kimchi prepared during 5 ℃ aging,

도 9는 5℃ 숙성중에서 제조김치들의 4주째의 관능평가를 나타낸 도이고, 9 is a diagram showing the sensory evaluation of the fourth week of the kimchi prepared during 5 ℃ aging,

도 10은 5℃ 숙성중에서 제조김치들의 6주째의 표면 효모 증식 상황을 나타 낸 도이다. 10 is a diagram showing the surface yeast growth situation of the 6th week of the kimchi prepared during 5 ℃ aging.

본 발명은 해양심층수를 이용하여 제조한 김치의 제조방법에 관한 것이다.The present invention relates to a method for producing kimchi prepared using deep sea water.

김치는 우리나라의 대표적인 전통발효식품으로 쌀 위주인 우리 식생활에서 가장 중요한 부식의 하나이다. 예부터 채소류가 나타나지 않은 겨울철에 주로 많이 담근 저장식품이지만, 지금은 냉장고 등 저장시설이 보편화 되면서 일년내내 섭취하는 상용식품으로 자리잡고 있다. Kimchi is a representative traditional fermented food in Korea and is one of the most important corrosion in our diet. It is a preserved food dipped mainly in winter, when vegetables have not appeared, but now it is becoming a commercial food that is consumed all year as storage facilities such as refrigerators become more common.

동물성 재료인 젓갈은 아미노산을 공급해 주는데, 밥에서 부족한 단백질을 보완해 준다. 또, 김치가 익으면서 새우젓, 멸치젓, 황석어젓등의 단백질이 아미노산으로 분해되어 공급원이 된다. Salted fish, an animal ingredient, provides amino acids, making up for protein that's lacking in rice. In addition, as kimchi ripens, proteins such as shrimp, anchovy, and yellow salted fish are decomposed into amino acids to provide a source.

채소에는 칼슘, 구리, 인, 철분, 소금 등의 무기질이 풍부하여 비타민 C는 물론이고 밥을 주식으로 하는 사람들에게 특히 필요한 비타민 B(thiamin)의 흡수를 돕는다.Vegetables are rich in minerals such as calcium, copper, phosphorus, iron, and salt, helping to absorb vitamin C as well as vitamin B (thiamin), which is especially needed for people who use rice as their staple food.

김치류는 채소류의 즙과 식염등의 복합작용으로 장내를 깨끗하게 해주고, 위장내의 단백질 분해효소인 펙틴(pectin) 분비를 촉진시키며, 소화ㆍ흡수작용을 도와서 장내 미생물분포를 정상화 시킨다.Kimchi cleanses the intestines by the complex action of vegetable juice and salt, promotes the secretion of pectin, a protease enzyme in the stomach, and helps digestion and absorption to normalize the intestinal microbial distribution.

또한, 채소자체에 많이 함유되어 있는 섬유소는 변비를 예방하고 장염이나 결장염 같은 질병을 예방해 준다.In addition, the fiber in the vegetable itself prevents constipation and diseases such as enteritis and colitis.

익은 김치는 유기산,알코올,에스텔을 생산하는 유기산 발효식품으로 식욕을 증진시킨다. Ripe kimchi is an organic acid fermented food that produces organic acids, alcohols, and esters.

특히, 김치가 익으면서 나오는 젖산균은 해로운 세균의 작용을 억제하며 새콤한 맛을 낼 뿐 아니라 창자속의 다른 균을 억제하여 이상발효를 막아주고, 병원균을 억제한다.In particular, lactic acid bacteria that come out while kimchi ripens to suppress the action of harmful bacteria and give a sour taste, as well as inhibit other bacteria in the intestine to prevent abnormal fermentation, and inhibit pathogens.

해양심층수라는 것은 해수 표면으로부터 200미터 이하의 해수를 일컫는 것으로, 광합성이 일어나지 않아 식물성장에 필요한 질소, 인, 규산 등의 무기영양소를 많이 포함하고 있으며, 대기나 화학물질에 의한 오염 및 태양균이나 일반세균에 오염되지 않으므로 해양성 세균수도 적어 물리적 청정성도 매우 뛰어나며, 필수 미량원소와 다양한 미네랄이 균형 있게 포함되어 있고, 또한 4대 미네랄(마그네슘, 칼슘, 칼륨, 나트륨)외 아연, 셀렌, 망간 등을 포함하여 스트레스나 체질 불량 등으로 발생되는 각종 질병에 대한 면역기능도 우수하며, 식수로 사용하는 지표수나 지하수 등에 비해 청정도가 매우 뛰어난 것으로 알려져 있다. Deep sea water refers to seawater 200 meters or less from the surface of the sea, and contains many inorganic nutrients such as nitrogen, phosphorus and silicic acid, which are necessary for plant growth because photosynthesis does not occur. Since it is not contaminated with general bacteria, it has a small number of marine bacteria, so it has excellent physical cleanliness, and it contains essential trace elements and various minerals in a balanced manner. It also contains zinc, selenium, manganese, etc. in addition to the four major minerals (magnesium, calcium, potassium, sodium). In addition, it is also known to have excellent immunity against various diseases caused by stress or constitution, and has excellent cleanliness compared to surface water or groundwater used for drinking water.

해양 오염이 심각한 현재의 상태에서 표층해수는 식음료로 적합하지 않으나, 해양심층수는 표면층에 비하여 생균수가 그다지 많지 않을뿐더러 병원성 생물이 조금도 포함되어 있지 않기 때문에 음료로 선택하는 경우에 안전성이 지극히 높다고 할 수 있다(일본특허공고 평7-34728호).In the current state of severe marine pollution, surface seawater is not suitable for food and beverage, but since deep seawater does not contain much viable bacteria and contains no pathogenic organisms compared to the surface layer, it is extremely safe when selected as a drink. (Japanese Patent Publication No. 7-34728).

이러한 해양심층수의 특징은 태양광이 도달하지 않는 심해에서는 영양 물질을 소비하는 식물플랑크톤이 없기 때문에 박테리아 등에 의하여 분해된 영양물질이 풍부하고 칼슘, 마그네슘 등의 미네랄이 다량 포함되어 있는 부영양성(미네랄성)이 있고, 표면 해수로부터 200미터 이하에는 유기물의 농도가 낮고, 대장균 또는 일반세균에 의한 오염이 없으며, 육지나 대기로부터의 화학물질에 의한 오염의 가능성도 적은 청정성을 갖고 있으며, 일년 내내 저온으로 그 변화가 적고 안정된 낮은 수온성을 갖고, 해양 심층수는 수 천년 동안 형성된 물이기 때문에 그 성질이 안정되어 있으며, 각종 효소들의 작용으로 인하여 숙성화되어 있는 숙성성을 갖고 있으며, 필수 미량원소나 다양한 미네랄 성분이 균형 있게 포함되어 있어 용존 되어 있는 금속이온들의 작용으로 활성 산소에 대한 탁월한 소거작용 효과 등의 특성을 갖고 있다고 알려져 있다.The characteristics of deep ocean water are eutrophic (mineral) which is rich in nutrients decomposed by bacteria and contains a large amount of minerals such as calcium and magnesium because there is no phytoplankton consuming nutrients in the deep sea where sunlight does not reach. ), Less than 200 meters from the surface seawater, low concentration of organic matter, no contamination by E. coli or common bacteria, and less cleanliness, less likely to be polluted by chemicals from land or air, Its stability is low and its water temperature is low, and since deep sea water is water that has been formed for thousands of years, its properties are stable, and it is matured due to the action of various enzymes. Of the metal ions dissolved in the balance It is known to have properties such as an excellent scavenging effect on active oxygen.

이러한 해양심층수는 해수를 가열 농축하고 그 농축액을 냉각한 후, 농축액으로부터 얻어진 결정분을 제거하고, 이를 농축액의 부피가 원래 부피의 1/1000이하로 농축될 때까지 지속시켜 얻어지는 것을 특징으로 하는 건강증진액 및 그 제조방법을 기재하고 있고 그 용액 일부를 음료수에 가함으로써 당뇨병, 알레르기성 질환, 심근경색 등의 현대병의 예방에 유효한 건강음료를 개시하고 있다(일본특허공보 제2580428호).This deep sea water is obtained by heating and concentrating the seawater, cooling the concentrate, and then removing the crystals obtained from the concentrate, and continuing it until the volume of the concentrate is concentrated to less than 1/1000 of the original volume. The enhancer and its manufacturing method are described, and a healthy drink effective for the prevention of modern diseases such as diabetes, allergic disease, myocardial infarction, etc. is disclosed by adding a part of the solution to the beverage (Japanese Patent Publication No. 2580428).

또한, 이 해양심층수에 염화나트륨 등의 전해질을 가하여 전기분해장치에서 전기분해를 시행하여 그 양극 쪽으로부터 얻어진 강산성 산화수(pH 2.4 내지 2.7이하에서 산화환원전위가 1000mV 이상)과 음극 쪽으로부터 얻어진 알칼리 환원수가 알려져 있는데, 강산성 산화수의 용도로서는 그 산화력과 산성에 의한 살균작용으로 이용하여 의료용 기구의 소독살균 및 식품의 살균소독에 이용되어 왔다, 알칼리 환원수에 대해서는 위장내의 이상발효, 만성설사, 소화불량, 제산 및 위산과다 등의 위장질환에 대하여 효능이 있다고 알려져 있다(일본특허공개공보 제2001-198575호 참조)In addition, an electrolyte such as sodium chloride is added to the deep sea water and subjected to electrolysis in an electrolysis device. The strongly acidic oxidized water obtained from the positive electrode side (the redox potential is higher than 1000 mV at pH 2.4 to 2.7 or lower) and the alkali reduced water obtained from the negative electrode side are obtained. It is known that the use of strongly acidic oxidized water has been used for disinfection sterilization of medical instruments and sterilization of foods due to its oxidizing power and acidic sterilization action. And gastrointestinal diseases, such as excessive stomach acid, are known to be effective (see Japanese Patent Laid-Open No. 2001-198575).

이웃 일본에서는 지자체에서 해양심층수의 취수, 산업화를 행하여 해양심층수의 청정성과 미네랄 풍부성, 맛 부여기능 등을 이용하여 400 여가지 식품제조에 활용되고 있으나, 아직 김치제조에 해양심층수가 활용된 사례는 없다. In neighboring Japan, local governments have taken in-depth marine water and industrialized it to make more than 400 foods using the deep sea water's purity, mineral abundance, and flavoring functions. none.

더더구나, 본 발명에서처럼 김치 제조시에 해양심층수를 활용함으로서 김치의 활발한 발효를 돕고, 건강에 유익한 유산균의 증식을 증가시켜주며, 동시에 발효시 혐오감을 주는 클라도스포리움(Cladosporium)속 등의 효모의 생성을 억제하는 우수한 방부성을 나타내고, 숙성 전반적으로 조직이 물러지지 않고 배추특유의 아삭아삭함이 유지되게 하면서, 짠맛은 완화시키는 마일드(mild)한 맛을 내는 데 해양심층수 농축액이 크게 기여한다는 결과의 발표는 찾아 볼 수 없었다.Furthermore, by utilizing the deep sea water in the production of kimchi as in the present invention, it helps active fermentation of kimchi, increases the proliferation of beneficial lactic acid bacteria, and at the same time gives a disgusting feeling during fermentation of the yeast of the genus Cladosporium Announced the result that the deep seawater concentrate contributes greatly to the mild taste that alleviates the saltiness while maintaining the excellent crispness that suppresses the production and keeps the cabbage peculiarly crisp throughout the ripening. Could not find.

이에 본 발명자들은 염도 측정, pH 측정, 산도 측정, TBA 값 측정, 미생물수 측정 및 관능평가를 통하여 해양심층수를 이용하여 제조한 김치의 우수한 저장성 및 조직감, 풍미 및 색택 등 종합적인 면의 우수성을 확인하여 본 발명을 완성하였다.Therefore, the present inventors confirmed the excellent storage properties, texture, flavor and color of kimchi prepared by using deep sea water through salinity measurement, pH measurement, acidity measurement, TBA value measurement, microbial water measurement and sensory evaluation. The present invention was completed.

본 발명의 목적은 해양심층수를 이용하여 저장성 및 품질이 향상된 김치를 제조하는 제조방법을 제공하는데 그 목적이 있다. An object of the present invention is to provide a manufacturing method for producing kimchi with improved storage and quality using deep sea water.

상기 목적에 따라, 본 발명은 해양심층수를 이용하여 김치를 제조하는 제조방법을 제공한다.In accordance with the above object, the present invention provides a manufacturing method for producing kimchi using deep sea water.

상세하게는, 1) 해양심층수를 사용하여 10 ~ 15%의 소금농도 농축액을 만드는 제 1단계; Specifically, 1) a first step of making a concentrated salt concentration of 10 to 15% using deep sea water;

2) 상기 제 1단계의 해양심층수 농축액에 깨끗이 씻어서 2 ~ 3 등분을 낸 채소를 4 ~ 6시간 동안 절이는 제 2단계;2) a second step of rinsing the deep seawater concentrate of the first step to rinse the vegetables in 2 to 3 portions for 4 to 6 hours;

3) 무와 파는 3.5 ~ 4.5cm의 크기로 썰고, 생강 및 마늘은 곱게 다져서, 채소 80 ~ 90 중량%, 무 1.0 ~ 2.0 중량%, 파 1.5 ~ 2.5 중량%, 생강 0.5 ~ 1.0 중량%, 마늘 1.0 ~ 2.0 중량%, 고춧가루 2.0 ~ 4.0 중량%, 설탕 0.5 ~ 1.5 중량% 및 멸치액젓 2.0 ~ 3.0 중량%로 혼합하여 양념을 준비하는 제 3단계;3) Cut radishes and green onions into the size of 3.5 ~ 4.5cm, and chop the ginger and garlic finely, vegetables 80 ~ 90% by weight, radish 1.0 ~ 2.0% by weight, leeks 1.5 ~ 2.5% by weight, ginger 0.5 ~ 1.0% by weight, garlic 1.0 ~ 2.0% by weight, red pepper powder 2.0 to 4.0% by weight, 0.5 to 1.5% by weight sugar and 2.0 to 3.0% by weight of anchovy sake to prepare a seasoning;

4) 상기 제 2단계의 절여진 채소를 채소무게의 2 ~ 3배의 물에서 5 ~ 6 시간동안 침지시키고, 깨끗한 물로 3회 세척하여 탈염시킨 다음, 배추의 물기를 탈수시키고, 상기 제 3단계의 양념과 버무리는 제 4단계;4) The pickled vegetables of the second step is immersed in water two to three times the weight of the vegetable for 5 to 6 hours, washed three times with clean water and desalted, followed by dehydration of the cabbage, and the third step Seasoning and Mixing of the Fourth Step;

5) 상기 제 4단계의 김치를 1 ~ 2 일 동안 숙성시키는 제 5단계를 포함함을 특징으로 하는 저장성 및 품질이 향상된 김치를 제조하는 제조방법을 제공한다.5) It provides a manufacturing method for producing kimchi with improved storage and quality, characterized in that it comprises a fifth step of aging the kimchi of the fourth step for 1 to 2 days.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명에 따른 김치의 제조는 본 발명의 분야에 공지되어 있는 통상의 방법 으로 제조가 가능한 바, 이를 구체적으로 설명하면, Production of kimchi according to the present invention can be prepared by a conventional method known in the field of the present invention.

1) 해양심층수를 사용하여 5 ~ 25%, 바람직하게는 10 ~ 20%, 보다 바람직하게는 10 ~ 15%의 소금농도 농축액을 만드는 제 1단계; 1) a first step of making a salt concentration concentrate of 5 to 25%, preferably 10 to 20%, more preferably 10 to 15% using deep sea water;

2) 상기 제 1단계의 해양심층수 농축액에 깨끗이 씻어서 2 ~ 4 등분, 바람직하게는 2 ~ 3 등분을 낸 채소를 2 ~ 10 시간, 바람직하게는 4 ~ 6시간 동안 절이는 제 2단계;2) a second step of rinsing the deep seawater concentrate of the first step and then pickling vegetables for 2 to 4 parts, preferably 2 to 3 parts, for 2 to 10 hours, preferably 4 to 6 hours;

3) 무와 파는 3 ~ 6 cm, 바람직하게는 3.5 ~ 4.5cm의 크기로 썰고, 생강 및 마늘은 곱게 다져서, 채소 75 ~ 95 중량%, 바람직하게는 80 ~ 90 중량%, 무 0.5 ~ 3 중량%, 바람직하게는 1.0 ~ 2.0 중량%, 파 1 ~ 4 중량%, 바람직하게는 1.5 ~ 2.5 중량%, 생강 0.1 ~ 2.0 중량%, 바람직하게는 0.5 ~ 1.0 중량%, 마늘 0.5 ~ 2.5 중량%, 바람직하게는 1.0 ~ 2.0 중량%, 고춧가루 1.0 ~ 5.0 중량%, 바람직하게는 2.0 ~ 4.0 중량%, 설탕 0.1 ~ 2.0 중량%, 바람직하게는 0.5 ~ 1.5 중량% 및 멸치액젓 1.0 ~ 4.0 중량%, 바람직하게는 2.0 ~ 3.0 중량%로 혼합하여 양념을 준비하는 제 3단계;3) Radish and leeks are cut into 3 ~ 6 cm, preferably 3.5 ~ 4.5cm in size, ginger and garlic finely chopped, 75 to 95% by weight of vegetables, preferably 80 to 90% by weight, radish 0.5 to 3% by weight , Preferably 1.0 to 2.0% by weight, leek 1 to 4% by weight, preferably 1.5 to 2.5% by weight, ginger 0.1 to 2.0% by weight, preferably 0.5 to 1.0% by weight, garlic 0.5 to 2.5% by weight, preferably Preferably 1.0 to 2.0% by weight, red pepper powder 1.0 to 5.0% by weight, preferably 2.0 to 4.0% by weight, sugar 0.1 to 2.0% by weight, preferably 0.5 to 1.5% by weight and anchovy fish sauce 1.0 to 4.0% by weight, preferably The third step of preparing the seasoning by mixing 2.0 to 3.0% by weight;

4) 상기 제 2단계의 절여진 채소를 채소무게의 1 ~ 4배, 바람직하게는 2 ~ 3배의 물에서 4 ~ 8시간, 바람직하게는 5 ~ 6 시간동안 침지시키고, 깨끗한 물로 3회 세척하여 탈염시킨 다음, 배추의 물기를 탈수시키고, 상기 제 3단계의 양념과 버무리는 제 4단계;4) The pickled vegetables of the second step are immersed in 1 to 4 times the vegetable weight, preferably 2 to 3 times the water for 4 to 8 hours, preferably 5 to 6 hours, washed three times with clean water After desalting by dehydration, the water of the Chinese cabbage is dehydrated, and the seasoning and mixing of the third step are carried out in a fourth step;

5) 상기 제 4단계의 김치를 1 ~ 4일, 바람직하게는 1 ~ 2 일 동안 숙성시키는 제 5단계를 포함하는 제조공정으로부터 본 발명의 해양심층수를 이용하여 제조 한 김치를 수득할 수 있다.5) Kimchi prepared using the deep sea water of the present invention can be obtained from the manufacturing process including the fifth step of ripening the kimchi of the fourth step for 1 to 4 days, preferably 1 to 2 days.

본 발명의 해양심층수는 바람직하게는 동해, 보다 바람직하게는 울릉도 인근 지역, 가장 바람직하게는 위도 37도, 경도 130도 지역의 수심, 바람직하게는 약 200 ~ 1,000m, 보다 바람직하게는 약 300 ~ 600m, 가장 바람직하게는 약 400 ~ 500m에서 취수한 것을 특징으로 한다.The deep sea water of the present invention is preferably in the East Sea, more preferably near Ulleungdo, most preferably 37 degrees latitude, 130 degrees longitude, preferably about 200 to 1,000 m, more preferably about 300 to It is characterized in that the water is taken at 600m, most preferably about 400 ~ 500m.

또한, 본원 발명은 상기 제조방법으로 수득되는 품질 개선 및 보존성 향상의 기능성 김치를 제공한다.In addition, the present invention provides a functional kimchi of improving the quality and storage properties obtained by the production method.

본 발명의 해양심층수를 이용하여 제조한 김치에 대하여 염도 측정, pH 측정, 산도 측정, TBA 값 측정, 미생물수 측정 및 관능평가를 실험한 결과, 우수한 방부성 및 조직감, 풍미 및 색택 등 종합적인 면에서 우수성을 나타냄을 확인하였다.Experimental results of salinity measurement, pH measurement, acidity measurement, TBA value, microbial water measurement and sensory evaluation of kimchi prepared using the deep sea water of the present invention, in terms of overall preservation and texture, flavor and color, etc. It was confirmed that the excellence was shown.

본 발명의 채소는 배추, 무, 열무, 무청, 순무, 오이, 파, 쪽파, 알타리, 호박, 박, 시금치, 가지, 당근, 양파, 쑥갓, 미나리, 부추, 풋고추, 고추잎, 콩잎, 팥잎, 깻잎, 콩나물, 돗나물, 돌나물, 참나물, 씀바귀나물, 달래나물, 고구마순, 고구마줄기, 마늘줄기, 토란 및 우엉을 포함한다.Vegetables of the present invention are Chinese cabbage, radish, radish, radish, turnip, cucumber, green onion, chives, altari, pumpkin, gourd, spinach, eggplant, carrots, onions, garland chrysanthemum, buttercups, leek, green pepper, red pepper leaves, soybean leaves, red beans, Includes sesame leaves, bean sprouts, edible greens, sedums, real greens, bitter greens, soothing greens, sweet potato sprouts, sweet potato stems, garlic stems, taro and burdock.

본 발명의 김치는 배추김치, 호박배추김치, 백김치, 파김치, 쪽파김치, 깍두기, 박깍두기, 무청깍두기, 굴깍두기, 숙깍두기, 강릉깍두기, 창란젓깍두기, 새우젓깍두기, 함경도대구깍두기, 물김치, 오이물김치, 미나리물김치, 인삼물김치, 귤물김치, 보쌈김치, 오이김치, 오이지, 오이소박이, 열무김치, 호박열무김치, 박김 치, 갓김치, 장김치, 나박김치, 묘삼나박김치, 동치미, 연꽃동치미, 알타리동치미, 알타리김치, 석밖지, 굴석박지, 무오이 송송이, 석류김치, 고수김치, 총각김치, 비늘김치, 숙김치, 용인외지, 채김치, 채칼김치, 순무짠지, 순무김치, 순무석밖지, Kimchi of the present invention, Chinese cabbage kimchi, pumpkin cabbage kimchi, white kimchi, green kimchi, scallion kimchi, diced, bakkkakdu, radish radish, oyster kkakeki, kkakdukdu, gangneung kkakdugi, changran jjigakdugi, shrimp jjigaegi, Hamgyeongdo Daegu kkakdugi, water kimchi, cucumber Water Kimchi, Buttercup Kimchi, Ginseng Water Kimchi, Tangerine Water Kimchi, Bossam Kimchi, Cucumber Kimchi, Cucumber, Cucumber Kimchi, Cucumber Kimchi, Pumpkin Radish Kimchi, Park Kimchi, Gad Kimchi, Jang Kimchi, Nabak Kimchi, Myosamnabakchi Kimchi, Dongchimi, Lotus Flower Kimchi , Altari Dongchimi, Altari Kimchi, Sukji, Oyster Foil, Cucumber Songsong, Pomegranate Kimchi, Coriander Kimchi, Bachelor Kimchi, Scale Kimchi, Simmered Kimchi, Yongin Oji, Chaekimchi, Chaekkal Kimchi, Turnip Salty Paper, Turnip Kimchi, Turnip Sukji,

마늘줄기김치, 고구마줄기김치, 고구마순김치, 고춧잎김치, 콩잎김치, 팥잎김치, 깻잎김치, 들깻잎짠지, 짠짠지, 파짠지, 무짠지, 골곰짠지, 해물김치, 감동김치, 감동젓김치, 명태김치, 한치김치, 전복김치, 서거리김치, 파래김치, 꼴뚜기무생채, 무청김치, 무말랭이김치, 미나리김치, 시금치김치, 가지김치, 당근김치, 양파김치, 풋고추김치, 부추김치, 콩나물김치, 우엉김치, 쑥갓김치, 토란김치, 씀바귀김치, 돌나물김치, 돗나물김치, 참나물김치, 어리김치, 솎은배추겉절이, 검들김치, 약김치, 고추젓김치, 달래젓김치, 고들빼기김치, 고들빼기조기젓김치, 고들빼기새우젓김치, 감김치, 반지(백지), 안동식혜, 가자미식해, 분홍지, 더덕지, 사연지, 돌뱅이국물김치, 비지미, 동지김치(꽃동배추김치), 퍼데기김치, 닭김치, 꿩김치, 가두배추섞박김치, 냉면김장김치, 겨자김치, 삶은무김치, 매화김치, 봄김치(햇김치) 및 풋절이를 포함한다. Garlic Stem Kimchi, Sweet Potato Stem Kimchi, Sweet Potato Kimchi, Red Pepper Leaf Kimchi, Bean Leaf Kimchi, Red Bean Leaf Kimchi, Sesame Leaf Kimchi, Perilla Leaf Woven, Salty Woven, Green Wedge, Radish, Golgoji, Seafood Kimchi, Stirred Kimchi, Stir-fried Kimchi, Myeongtae Kimchi, Hanchi kimchi, abalone kimchi, seogeori kimchi, green kimchi, radish kimchi, radish kimchi, radish kimchi, buttercup kimchi, spinach kimchi, eggplant kimchi, carrot kimchi, onion kimchi, green pepper kimchi, leek kimchi, bean sprout kimchi, burdock kimchi, mugwort Kimchi, taro kimchi, mochi kimchi, dolnamul kimchi, tongnaul kimchi, real green kimchi, young kimchi, red cabbage kimchi, black kimchi, medicinal kimchi, red pepper kimchi, dal kimchi, dolgi kimchi, saddle young kimchi, , Kimchi, Ring (Blank paper), Andong Sikhye, Fluke, Pink Paper, Deodeokji, Sayeonji, Dolbang Soup Kimchi, Bijimi, Winter solstice Kimchi, Pudding Kimchi, Chicken Kimchi, Pheasant Kimchi, Mixed cabbage kimchi, cold noodle And a kimchi, mustard kimchi, boiled mugimchi, plum kimchi, sauerkraut spring (haetgimchi) and the foot section.

이하, 본 발명을 실시예 및 실험예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by Examples and Experimental Examples.

단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 의해 한정되는 것은 아니다.However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

참고예Reference Example 1. 시료의 준비 1. Preparation of Sample

배추, 무 및 파는 강릉 택지에 위치한 하나로 마트에서, 생강은 강릉 중앙시장 기름상회에서 구입하였고, 고춧가루는 일직농협안동농산물 공동가공소에서 제조된 고춧가루를 사용하였으며, 액젓은 (주)대상식품, 간 마늘은 (주)CJ주식회사, 천일염은 (주)신동방, 설탕은 (주)CJ주식회사 및 꽃소금은 (주) 대한 염업에서 제조된 것을 사용하였다.Chinese cabbage, radish, and leeks were purchased at Hanaro Mart, located in Gangneung, and Ginger was purchased at the Gangneung Central Market Oil Market. The red pepper powder was made from red pepper powder manufactured by Iljik Agricultural Cooperative Andong Agricultural Products Processing Center. Silver CJ Co., Ltd., Shin-Dong-Bang Co., Ltd. and Sugar Salt CJ Co., Ltd. and Flower Salt Co., Ltd. were used in Daehan Salt Industry.

참고예Reference Example 2. 시약, 기기 및 장치 2. Reagents, Instruments and Devices

실험에 사용한 시약으로 0.1N 수산화나트륨(NaOH), 트리클로로아세트산(Trichloroacetic acid), 인산(phosphoric acid) 및 TBA(thiobarbituric acid)는 일급 시약을 사용하였고, 와트만(Whatman) No.1,2 여과지, 볼텍스 믹서(vortex mixer; KMC-1300V, Vision Scientific Co., Korea), 원심분리기(Mega21R, Hanil Science industrial, Korea), 균질기(Homogenizer; Jk ultra TuRRAX T25 basic Ika Laboretehnik, T25B, IKA Works(Asia) sdn. Bhd, Malaysia), pH 미터(pH meter; pH526, Mettler Toledo, Swiss), 오토클레이브(autoclave; MLS-3020, Sanyo, Japan), 클린벤치(clean bench; VS-1400LS, Vision Scientific Co., Korea) 및 UV(UV1601, SHIMADZU, Japan) 등의 실험기기 및 장치를 사용하였다.0.1N sodium hydroxide (NaOH), Trichloroacetic acid, phosphoric acid and thiobarbituric acid were used as the reagents used in the experiment. Whatman No.1,2 filter paper was used. , Vortex mixer (KMC-1300V, Vision Scientific Co., Korea), centrifuge (Mega21R, Hanil Science industrial, Korea), homogenizer (Homogenizer; Jk ultra TuRRAX T25 basic Ika Laboretehnik, T25B, IKA Works (Asia) sdn.Bhd, Malaysia), pH meter (pH meter; pH526, Mettler Toledo, Swiss), autoclave (MLS-3020, Sanyo, Japan), clean bench (VS-1400LS, Vision Scientific Co. , Korea) and UV (UV1601, SHIMADZU, Japan) and the like were used.

실시예Example 1.  One. 해양심층수Deep ocean water 김치의 제조 Manufacture of Kimchi

상기 참고예 1의 천일염, 정제염, 표층해수 및 해양심층수를 이용하여 각각 10% 소금농도 농축액을 만들어 놓는다.A 10% salt concentration concentrate is prepared using the natural salt, purified salt, superficial seawater, and deep sea water of Reference Example 1, respectively.

배추 5통, 무 1개 및 파 1단을 깨끗이 씻어서 이물질을 제거한 후, 배추는 칼로 넓이 4㎝되게 나박으로 자르고 무와 파는 4㎝ 길이로 채썰기하고 준비해놓는다. 생강 및 마늘은 곱게 다져 놓는다.After washing 5 barrels of cabbage, 1 radish, and 1 step of leek to remove foreign substances, cut the cabbage into 4cm wide nabak with a knife, and chop radishes and leeks into 4cm long and prepare. Ginger and garlic are finely chopped.

상기의 천일염, 정제염, 표층해수 및 해양심층수를 이용하여 제조한 각각의 10% 소금농도 농축액에 상기의 배추를 18~20℃의 상온에서 배추무게의 2배의 물에서 6시간 동안 침지시키고, 깨끗한 물로 3회 세척하여 탈염시킨 다음, 배추의 물기를 탈수시켰다.The above cabbage was immersed for 6 hours in twice the water of cabbage at room temperature of 18 ~ 20 ℃ in each 10% salt concentration concentrate prepared using the sun salt, purified salt, superficial sea water and deep sea water. After washing three times with water to desalt, the water of the cabbage was dehydrated.

상기의 탈수처리한 배추에 고춧가루, 생강, 마늘, 액젓, 무, 파 및 설탕 등의 양념을 하기 표 1의 구성대로 하여 혼합한 후, 혼합한 양념속을 배추에 버무려서 김치를 제조하였다. Seasonings such as red pepper powder, ginger, garlic, fish sauce, radish, green onion, and sugar to the dehydrated cabbage were mixed according to the configuration of Table 1 below, and the mixed seasoning was mixed with cabbage to prepare kimchi.

소금물에 절인 후 물빠진 중량을 기준으로 양념의 양 The amount of seasonings based on the weight of the water after pickling in salt water 성 분(Ingredients) Ingredients 비 율(Ratio, %)Ratio (%) 고춧가루(Red pepper powder) Red pepper powder 3.53.5 마늘(Garlic) Garlic 1.41.4 생강(Ginger) Ginger 0.60.6 멸치액젓(Anchovy juice) Anchovy juice 2.22.2 무(Radish) Radish 1.31.3 파(Leek) Leek 22 설탕(Sugar) Sugar 1One

상기의 제조된 김치를 플라스틱용기에 넣고 5℃의 온도에서 저장하여 저장 시간에 따라 염도, pH, 산도, TBA값, 미생물 수 및 관능평가 등을 하기와 같이 살펴보았다.Put the prepared kimchi in a plastic container and stored at a temperature of 5 ℃ looked at salinity, pH, acidity, TBA value, microbial number and sensory evaluation according to the storage time as follows.

실험예 1. 농축액에 따른 김치의 염도 측정 실험Experimental Example 1. Experiment of Salinity Measurement of Kimchi According to Concentrate

식품 중의 식염의 양을 알아내는 데는 두 가지 방법이 있다. 즉, 나트륨(Na)를 정량하여 염화나트륨(NaCl)으로 환산하거나 염소(Cl)을 정량하여 염화나트륨으로 환산하는 것이다. 대개의 식염정량실험은 염소를 정량하여 식염량을 추정하는 것이 일반적인 방법으로 본 발명에서는 이 방법을 이용하여 상기 실시예 1의 천일염, 정제염, 표층해수 및 해양심층수 농축액을 사용하여 제조한 각각의 김치들에 대하여 염도를 하기와 같이 측정하였다(채수규 외 4명, 표준식품분석학, 지구문화사, pp460-463, 1999).There are two ways to determine the amount of salt in food. That is, sodium (Na) is quantified and converted into sodium chloride (NaCl), or chlorine (Cl) is quantified and converted into sodium chloride. In most saline quantitative experiments, it is common to estimate chlorine by quantifying chlorine. In the present invention, each kimchi prepared by using the sun salt, purified salt, superficial seawater, and deep sea water concentrate of Example 1 using this method is used. About the field Salinity was measured as follows (Chae, Su-Kyu et al., Standard Food Analysis, Geocultural History, pp460-463, 1999).

시료 10g을 정확히 칭취하여 물 20ml를 가하여 균질기(homogenizer)로 마쇄하여 이것을 100ml 메스플라스크에 옮겨 정용하였다. 10 g of the sample was accurately weighed, and 20 ml of water was added thereto, followed by grinding with a homogenizer, which was transferred to a 100 ml volumetric flask to be used.

정용한 후, 약 5분간 메스플라스크를 흔들어 균질화하였다. 그 후에 건조여과지로 여과하여 시료용액으로 하였다.After the dialysis, the volumetric flask was shaken and homogenized for about 5 minutes. Thereafter, the filtrate was filtered through dry filter paper to obtain a sample solution.

이상과 같이 조제한 시료용액 10ml를 100ml 삼각플라스크에 정확히 취하고, 10% 중크롬산칼륨(K2CrO4) 지시약 1ml를 가하고 0.1N-질산은(AgNO3) 표준용액으로 적정하였다. 10 ml of the sample solution prepared as described above was accurately taken into a 100 ml Erlenmeyer flask, 1 ml of 10% potassium dichromate (K 2 CrO 4 ) indicator was added, and titrated with 0.1 N-silver nitrate (AgNO 3 ) standard solution.

시료 중의 염소 및 식염의 함량은 하기 수학식 1에 따라 계산하였다(도 1 참조).The content of chlorine and salt in the sample was calculated according to Equation 1 below (see FIG. 1).

NaCl(%) = 0.00585 × V × F × D × 100 / SNaCl (%) = 0.00585 × V × F × D × 100 / S

V: 0.1N-질산은(AgNO3) 용액의 적정소비량(ml)V: titration consumption of 0.1 N silver nitrate (AgNO 3 ) solution (ml)

F: 0.1N-질산은(AgNO3) 용액의 역가F: Titer of 0.1 N-silver nitrate (AgNO 3 ) solution

D: 희석배수D: dilution factor

S: 시료 채취량(g)S: sampling amount (g)

3.546: 0.1N-질산은(AgNO3) 용액 1ml에 상당하는 염소(Cl)의 양(mg)3.546: 0.1 N-silver nitrate (AgNO 3 ) Chlorine (Cl) equivalent to 1 ml of solution (mg)

0.00585: 0.1N-질산은(AgNO3) 용액 1ml에 상당하는 식염(NaCl)의 양(g)0.00585: Amount of salt (gCl) equivalent to 1 ml of 0.1 N-silver nitrate (AgNO 3 ) solution

실험예 2. 농축액에 따른 김치의 pH측정Experimental Example 2. pH measurement of kimchi according to the concentrate

상기 실시예 1의 천일염, 정제염, 표층해수 및 해양심층수 농축액을 사용하여 제조한 각각의 김치들에 대하여 pH를 하기와 같이 측정하였다(채수규 외 4명, 표준식품분석학, 지구문화사, pp133-136, 1999).For each kimchi prepared using the natural salt, purified salt, superficial seawater and deep seawater concentrate of Example 1 pH was measured as follows (Chae, Su-Kyu et al., Standard Food Analysis, Geocultural History, pp133-136, 1999).

시료 5g과 증류수 45ml를 취하여 균질기(Homogenizer)로 마쇄한 후,마쇄한 시료를 여과지 No. 2(125mm)로 여과시킨 다음, 이 여과액으로 pH 미터(meter)를 이용하여 pH를 측정하였다.5 g of sample and 45 ml of distilled water were taken and ground by a homogenizer, and then the ground sample was filtered. After filtering to 2 (125mm), the pH was measured using a pH meter with the filtrate.

그 실험결과, 동일한 염도조건인 해양심층수 농축액 절임김치, 표층해수 농축액 절임 김치를 천일염, 정제염 농축액 절임김치와 비교해 보면, pH면에서는 큰 차이가 없었다(도 2 참조).As a result of the experiment, compared with the deep seawater concentrate pickled kimchi and superficial seawater concentrate pickled kimchi, which are the same salinity conditions, compared with the sun salt and the pickled kimchi with purified salt concentrate, there was no significant difference in pH (see FIG. 2).

실험예 3. 농축액에 따른 김치의 산도 측정 실험Experimental Example 3 Experiment of Acidity Measurement of Kimchi According to Concentrate

식품 100g을 태워서 얻은 회분을 중화시키는 데 필요한 1N의 산 의 ml수를 그 식품의 산도라 한다.The number of ml of 1 N acid required to neutralize the ash obtained by burning 100 g of food is called the pH of the food.

이것을 측정하기 위해서는 식품의 일정량을 회화시켜 얻은 식품의 회분을 일정농도의 염산용액에 용해시켜서 알칼리표준용액으로 중화시키고, 이 때 사용한 알칼리의 양으로부터 산도를 계산한다.In order to measure this, the ash of the food obtained by incubating a certain amount of food is dissolved in a constant concentration of hydrochloric acid solution and neutralized with an alkaline standard solution, and the acidity is calculated from the amount of alkali used at this time.

상기 실시예 1의 천일염, 정제염, 표층해수 및 해양심층수 농축액을 사용하여 제조한 각각의 김치들에 대하여 산도를 하기와 같이 측정하였다(채수규 외 4명, 표준식품분석학, 지구문화사, pp490-492, 1999).For each kimchi prepared using the natural salt, purified salt, superficial seawater and deep seawater concentrate of Example 1 Acidity was measured as follows (Chae, Su-Kyu et al., Standard Food Analysis, Geocultural History, pp490-492, 1999).

시료 15g과 증류수 15ml를 취하여 균질기(Homogenizer)로 마쇄한 다음, 마쇄한 시료를 여과지 No. 2(125mm)로 여과시켰다.15 g of sample and 15 ml of distilled water were taken and crushed with a homogenizer. It was filtered to 2 (125 mm).

상기의 여과시킨 액을 0.1N 수산화나트륨(NaOH)으로 적정하여 pH가 8.3이 될 때까지 소비된 0.1N 수산화나트륨(NaOH)의 양을 정량하여 하기 수학식 2에 따라 계산하였다.The filtered solution was titrated with 0.1 N sodium hydroxide (NaOH) to quantify the amount of 0.1 N sodium hydroxide (NaOH) consumed until the pH was 8.3 was calculated according to the following equation (2).

산도(%) = 0.1N NaOH소비량× NaOH 역가(factor)× 0.009× D.W(ml)× 100/시료량(g)Acidity (%) = 0.1 N NaOH Consumption × NaOH Factor × 0.009 × D.W (ml) × 100 / Sample (g)

그 실험결과, 동일한 염도조건인 해양심층수 농축액 절임김치, 표층해수 농축액 절임 김치를 천일염, 정제염 농축액 절임김치와 비교해 보면, 숙성정도를 나타내는 산도면에서는 큰 차이가 없었다(도 3 참조).As a result, when compared to the deep seawater concentrate pickled kimchi, superficial seawater concentrate pickled kimchi, which is the same salinity conditions, compared with sun salt and pickled kimchi with purified salt concentrate, there was no significant difference in acidity showing the degree of ripening (see FIG. 3).

실험예Experimental Example 4. 농축액에 따른 김치의  4. Kimchi according to the concentrate TBATBA 값 측정 실험Value measurement experiment

TBA값은 유지의 산패 정도를 알아보기 위한 비색분석법으로 UV-스펙트로미터(spectrometer)로 용액의 색의 농도를 측정하여 물질을 정량하는 방법이다. The TBA value is a colorimetric method for determining the degree of rancidity of oils and fats and is a method of quantifying a substance by measuring the color concentration of a solution by using a UV-spectrometer.

상기 실시예 1의 천일염, 정제염, 표층해수 및 해양심층수 농축액을 사용하여 제조한 각각의 김치들에 대하여 TBA값을 하기와 같이 측정하였다(채수규 외 4명, 표준식품분석학, 지구문화사, pp350-351, 1999).For each kimchi prepared using the natural salt, purified salt, superficial seawater and deep seawater concentrate of Example 1 TBA values were measured as follows (Chae Su-kyu et al., 4, Standard Food Analysis, Geocultural History, pp350-351, 1999).

시료 20g과 2M 인산(phosphoric acid)이 들어있는 4℃로 냉각된 20% 트리클로로아세트산(Trichloroacetic acid) 용액 50ml를 취하여 1.5분간 균질하게 마쇄시켰다. 마쇄액을 100ml 정용 플라스크에 옮긴 후, 증류수로 100ml로 정용한 다음, 흔들어 혼합하였다.50 ml of 20% Trichloroacetic acid solution cooled to 4 ° C. containing 20 g of sample and 2 M phosphoric acid (phosphoric acid) were taken and ground homogeneously for 1.5 minutes. The grinding solution was transferred to a 100 ml flask, which was then diluted to 100 ml with distilled water and shaken to mix.

상기 혼합액 50ml를 와트만(Whatman) No.1여과지로 여과한 후, 원심분리기 15,000ppm에서 10분간 돌린 다음, 상층액만 5ml 취해 실린지로 여과하여 시험관에 옮겼다. After 50 ml of the mixed solution was filtered through Whatman No. 1 filter paper, the mixture was turned for 10 minutes at 15,000 ppm of a centrifuge, and only 5 ml of the supernatant was filtered through a syringe and transferred to a test tube.

0.005m 2-TBA(thiobarbituric acid) 5ml를 넣은 후, 마개를 막고 흔들어 혼합(vortexing)한 다음, 암소에서 상온으로 15시간 방치하였다. 발색된 액을 530nm 에서 UV로 흡광도를 측정하였다.After adding 5 ml of 0.005m 2-TBA (thiobarbituric acid), the cap was capped and vortexed, and then left at room temperature for 15 hours in the dark. The absorbance of the developed solution was measured by UV at 530 nm.

그 실험결과, 유지의 산패도를 나타내는 TBA값은 숙성 3주째를 기준으로 할 때, 크게 차이가 나타났다(도 4 참조).As a result of the experiment, the TBA value indicating the fat loss of fats and oils was significantly different based on the 3rd week of aging (see FIG. 4).

실험예 5. 농축액에 따른 김치의 선도유지효과 Experimental Example 5. Freshness Effect of Kimchi According to Concentrate

상기 실시예 1의 천일염, 정제염, 표층해수 및 해양심층수 농축액을 사용하여 제조한 각각의 김치들에 대하여 생균수, 곰팡이수 및 유산균수를 하기와 같이 측정하였다(한국식품공업협회, 식품공전(개정판), p97, pp106-107).For each kimchi prepared using the natural salt, purified salt, superficial seawater and deep seawater concentrate of Example 1 The number of viable cells, the number of fungi and the number of lactic acid bacteria were measured as follows (Korea Food Industry Association, Food Code (Revised Edition), p97, pp106-107).

미생물 총균의 분석은 전체 생균계수(total counts), 효모곰팡이(yeast and mold) 및 유산균군(Leuconostoc Lactobasillus)을 조사하였다. The total microbial count was examined for total counts, yeast and mold and lactic acid bacteria ( Leuconostoc and Lactobasillus) .

각각의 시료 10 g과 살균한 0.1% 펩톤(peptone)수 90ml를 스토마커 백(stomacher bag)에 넣고 스토마커(stomacher; Lab blender, interscience, France)를 사용하여 2분 동안 균질화한 후, 펩톤수에 단계 희석하여 일반 세균은 PCA(plate count agar; DIFCO) 플레이트, 효모ㆍ곰팡이는 PDA(potato dextrose agar; DIFCO) 플레이트 및 유산균은 MRS( Lactobacilli MRS Broth; DIFCO) 플레이트와 m-LBS(modified-LBS ;DIFCO) 플레이트에 각각 0.1ml를 분주한 후, 도말하였다. 10 g of each sample and 90 ml of sterilized 0.1% peptone water were placed in a stomacher bag and homogenized using a stomacher (Lab blender, interscience, France) for 2 minutes. Dilutions were carried out in the normal phase, so that general bacteria were plate count agar (DIFCO) plates, yeasts and molds (potato dextrose agar; DIFCO) plates, and lactic acid bacteria were Lactobacilli MRS Broth (DIFCO) plates and m-LBS (modified-LBS). ; DIFCO) plates were each dispensed with 0.1 ml and plated.

PCA 플레이트는 35℃에서 24시간 배양하여 콜로니를 계수하여 측정하였고, PDA 플레이트는 25℃에서 48시간 배양 후 콜로니를 계수하였으며, MRS 플레이트(Lactobacilli species 배양배지)는 37℃에서 48시간 배양 후 콜로니를 계수하였 고, m-LBS 플레이트는 30℃에서 72시간 배양 후 콜로니를 계수하여 log CFU/g으로 나타내었다. PCA plates were cultured at 35 ° C. for 24 hours to count colonies. PDA plates were cultured at 25 ° C. for 48 hours, and colonies were counted. MRS plates ( Lactobacilli species culture medium) were cultured at 37 ° C. for 48 hours to obtain colonies. The m-LBS plate was counted as log CFU / g after 72 hours of incubation at 30 ° C.

그 실험결과, 생균수 증식에서는 시료간에 큰 차이를 보이지 않았으나, 발효와 관련되는 곰팡이수에서는 해양심층수 농축액 절임김치가 우수하였다(도 5 및 도 6 참조). As a result of the experiment, the growth of viable cell number did not show a big difference between the samples, but the deep seawater concentrate pickled kimchi was excellent in the fungus water related to fermentation (see FIGS. 5 and 6).

한편, 숙성중 유산균수면에서는 류코노스탁(Leuconostoc)속의 경우는 숙성 4주째까지 기준으로 볼 때, 표층해수 농축액 절임김치나 해양심층수 농축액 절임김치의 경우에 별반 차이를 보이지 않았고, 천일염 농축액 절임 김치는 4주째에 증식이 현저히 낮아지는 경향을 보였다(도 7 참조). On the other hand, in the case of lactic acid bacteria during ripening, the Leuconostoc genus did not show any difference between the superficial seawater pickled kimchi and the deep seawater concentrate pickled kimchi, based on the fourth week of fermentation. At 4 weeks, the proliferation tended to be significantly lower (see FIG. 7).

반면, 유산균의 대표격인 락토바실러스(Lactobasillus)속은 숙성전체적으로 해양심층수 농축액 절임김치쪽이 다소 많은 증식을 나타내었다(도 8 참조).On the other hand, Lactobasillus genus , which is a representative example of lactic acid bacteria, showed more proliferation in pickled kimchi in the deep sea water concentrate as a whole (see FIG. 8).

실험예 6. 농축액에 따른 김치의 관능 검사Experimental Example 6. Sensory test of kimchi according to the concentrate

상기 실시예 1의 천일염, 정제염, 표층해수 및 해양심층수 농축액을 사용하여 제조한 각각의 김치들에 대하여 강릉대 식품과학과 학생 10명을 대상으로 하여 관능 검사를 하기와 같이 실시하였다.For each kimchi prepared using the natural salt, purified salt, superficial seawater and deep seawater concentrate of Example 1 The sensory test was conducted for 10 students from the Department of Food Science, Gangneung University.

그 실험결과, 숙성 4주째에서 조직감, 풍미, 색택 및 종합평가면으로 볼 때, 해양심층수 농축액을 사용하여 제조한 김치가 가장 우수하였다. As a result, in the fourth week of aging, kimchi prepared using deep seawater concentrate was the best in terms of texture, flavor, color selection and comprehensive evaluation.

특히, 해양심층수 농축액을 사용하여 제조한 김치가 조직감면에서 아삭아삭함이 우수하였고, 동일한 염도이었는데도 불구하고, 타 농축액을 사용하여 제조한 김치들에 비해 짜지 않으면서도 전체적인 맛이 우수하였다(도 9 참조). In particular, kimchi prepared using deep seawater concentrate was excellent in crispness in texture reduction, and despite the same salinity, the overall taste was excellent without being salty compared to kimchi prepared using other concentrates (see FIG. 9). ).

결론적으로, 이러한 결과는 해양심층수의 성분인 풍부한 질산이온-질소(NO3-N), 인산이온-인(PO4-P) 및 이산화규소-규소(SiO2-Si) 등이 김치의 활발한 발효를 도우면서, 건강에 유익한 유산균의 증식을 증가시켜주고, 동시에 발효시 혐오감을 주는 클라도스포리움(Cladosporium)속 등의 효모의 생성을 억제하는 우수한 방부성까지 나타내는 것으로 사료된다(도 10 참조). In conclusion, these results indicate that the rich fermentation of Kimchi is due to the rich nitrate-nitrogen (NO 3 -N), phosphate-phosphorus (PO 4 -P) and silicon dioxide-silicon (SiO 2 -Si) components of deep sea water. In addition, it is believed that it increases the proliferation of lactic acid bacteria, which are beneficial to health, and at the same time, exhibits excellent antiseptic properties that suppress the production of yeasts such as the genus Cladosporium, which gives a sense of aversion during fermentation (see FIG. 10).

또한, 풍부한 미네랄성에 기인하는 이런 해양심층수의 저장성이 낮은 TBA값을 유도하고, 숙성 전반적으로 조직이 물러지지 않고 배추특유의 아삭아삭함이 유지되게 하면서, 짠맛은 완화시키는 마일드(mild)한 맛부여까지 영향을 미치기 때문에 조직감, 풍미 및 색택 등 종합적인 면에서 우수한 김치를 제조할 수 있는 것으로 사료된다.In addition, the deep storage of these deep sea waters, due to their rich minerality, induces low TBA values, and keeps the cabbage-specific crispness of the cabbage intact throughout the aging process, while maintaining a mild taste that eases the salty taste. It is thought to be able to produce excellent kimchi in overall aspects such as texture, flavor and color selection because of its influence.

이상에서 살펴본 바와 같이, 본 발명의 해양심층수를 이용하여 제조된 김치는 저장성 및 품질이 우수하고, 동시에 인체에 필수적인 무기염류 및 미네랄을 섭취할 수 있는 이점이 있기 때문에 식품학적 가치를 향상시키므로 국민들의 건강증진에 기여할 수 있다. As described above, kimchi prepared by using the deep sea water of the present invention has excellent storage properties and quality, and at the same time has an advantage of ingesting inorganic salts and minerals essential to the human body, thereby improving the food value of the people. It can contribute to health promotion.

Claims (5)

1) 해양심층수를 사용하여 10 ~ 15%의 소금농도 농축액을 만드는 제 1단계; 1) a first step of making a concentrated salt concentration of 10 to 15% using deep sea water; 2) 상기 제 1단계의 해양심층수 농축액에 깨끗이 씻어서 2 ~ 3 등분을 낸 채소를 4 ~ 6시간 동안 절이는 제 2단계;2) a second step of rinsing the deep seawater concentrate of the first step to rinse the vegetables in 2 to 3 portions for 4 to 6 hours; 3) 무와 파는 3.5 ~ 4.5cm의 크기로 썰고, 생강 및 마늘은 곱게 다져서, 채소 80 ~ 90 중량%, 무 1.0 ~ 2.0 중량%, 파 1.5 ~ 2.5 중량%, 생강 0.5 ~ 1.0 중량%, 마늘 1.0 ~ 2.0 중량%, 고춧가루 2.0 ~ 4.0 중량%, 설탕 0.5 ~ 1.5 중량% 및 멸치액젓 2.0 ~ 3.0 중량%로 혼합하여 양념을 준비하는 제 3단계;3) Cut radishes and green onions into the size of 3.5 ~ 4.5cm, and chop the ginger and garlic finely, vegetables 80 ~ 90% by weight, radish 1.0 ~ 2.0% by weight, leeks 1.5 ~ 2.5% by weight, ginger 0.5 ~ 1.0% by weight, garlic 1.0 ~ 2.0% by weight, red pepper powder 2.0 to 4.0% by weight, 0.5 to 1.5% by weight sugar and 2.0 to 3.0% by weight of anchovy sake to prepare a seasoning; 4) 상기 제 2단계의 절여진 채소를 채소무게의 2 ~ 3배의 물에서 5 ~ 6 시간동안 침지시키고, 깨끗한 물로 3회 세척하여 탈염시킨 다음, 배추의 물기를 탈수시키고, 상기 제 3단계의 양념과 버무리는 제 4단계;4) The pickled vegetables of the second step is immersed in water two to three times the weight of the vegetable for 5 to 6 hours, washed three times with clean water and desalted, followed by dehydration of the cabbage, and the third step Seasoning and Mixing of the Fourth Step; 5) 상기 제 4단계의 김치를 1 ~ 2 일 동안 숙성시키는 제 5단계를 포함함을 특징으로 하는 저장성 및 품질이 향상된 김치를 제조하는 제조방법.5) A manufacturing method for producing kimchi with improved shelf life and quality, comprising a fifth step of ripening the kimchi of the fourth step for 1 to 2 days. 제 1항에 있어서, 상기 해양심층수는 동해의 표층해수 아래에서 취수한 것을 특징으로 하는 제조방법. The method of claim 1, wherein the deep sea water is collected under surface seawater in the East Sea. 제 2항에 있어서, 상기 동해는 위도 37도, 경도 130도 지역임을 특징으로 하는 제조방법.The method of claim 2, wherein the East Sea is a latitude of 37 degrees, longitude 130 degrees region. 제 2항에 있어서, 상기 해양심층수는 수심 200 ~ 1,000m에서 취수한 것임을 특징으로 하는 제조방법.The method of claim 2, wherein the deep sea water is taken from a depth of 200 ~ 1,000m. 제 1항의 제조방법으로 수득되는 품질 개선 및 보존성 향상의 기능성 김치.Functional kimchi of the quality improvement and preservation improvement obtained by the manufacturing method of claim 1.
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