KR20080027935A - Endoscope accessory, biotissue analytical processing system and method of sampling for tissue analytical processing - Google Patents

Abstract

Endoscope accessory (1) comprising slender insertion part (15) inserted in a living body through accessory channel (10) of endoscope (2); tubular puncture needle (16) disposed at the distal end portion of the insertion part (15) and inserted in a biotissue; tissue sampling means (18) for, with the puncture needle (16) inserted in a biotissue, sampling a biotissue; and tissue treating means (22) for applying treatment for analysis to the biotissue sampled by the tissue sampling means (18). ® KIPO & WIPO 2008

Description

ENDOSCOPE ACCESSORY, BIOTISSUE ANALYTICAL PROCESSING SYSTEM AND METHOD OF SAMPLING FOR TISSUE ANALYTICAL PROCESSING}

The present invention relates to an endoscope treatment instrument inserted in a living body through a treatment instrument channel of an endoscope and used in combination with an endoscope, a biological tissue analysis processing system, and a sampling method for tissue analysis processing.

In general, biological tissues are collected and biological molecules are analyzed. In the analysis process of this kind of biological tissue, the mechanism described conventionally, for example in Unexamined-Japanese-Patent No. 2001-275947 (patent document 1) is used. Here, an endoscope puncture needle inserted into the living body through the treatment instrument channel of the endoscope for observing the living body is used. This endoscope puncture needle is inserted into a living body under observation by an endoscope, and punctures in a state where it sticks to a desired living tissue to collect the living tissue. The biological tissue collected here is returned to an examination site separate from the endoscope examination room, and examination of pathology diagnosis etc. is performed.

In the apparatus of Patent Literature 1, it takes a long time from the collection of biological tissue to the diagnosis. Therefore, there is a possibility that the state of biological tissues may deteriorate, and it is difficult to make an accurate diagnosis.

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and an object thereof is to provide an endoscope treatment instrument, a biological tissue analysis processing system, and a tissue analysis processing for collecting the collected biological tissue in a fresh state and performing accurate diagnosis. To provide a way.

An endoscope treatment instrument in one aspect of the present invention includes an elongated insertion portion inserted into a living body through a treatment instrument channel of an endoscope for observing the living body, and a tube disposed at the tip of the insertion portion and inserted into a living tissue. A puncture needle having a shape, a tissue collecting means for collecting a living tissue in a state where the puncture needle is inserted into a living tissue, and a tissue processing means for performing analytical processing on the biological tissue collected by the tissue collecting means. It is provided.

In the above aspect, the tubular puncture needle disposed at the distal end of the elongated insertion portion inserted into the living body through the treatment instrument channel of the endoscope for observing the living body is inserted into the living tissue. In the state where the puncture needle is inserted into the living tissue, the living tissue is collected by the tissue collecting means. The biological tissue collected after that is subjected to analytical processing by a tissue processing means.

The insertion portion has an elongated tubular body, the puncture needle is formed at the distal end of the tubular body, and the tissue collecting means includes an outer cylinder member detachably connected to a proximal end of the tubular body, and an axial direction in the barrel of the outer cylinder member. And a axial piston member slidably inserted therein, wherein the living body is moved inside the outer cylinder member through the puncture needle and the tubular body by a suction operation of sliding the piston member outwardly of the outer cylinder member. It is preferable that a syringe to be collected is provided, and the tissue processing means has a freezing device for freezing the biological tissue collected into the syringe.

And in the said structure, the outer cylinder member of the tissue collection means which is a syringe can be detachably connected to the base end of the elongate tubular body in which the puncture needle was formed in the front end part, and an axial piston member can slide in an axial direction in the cylinder of an outer cylinder member. Insert it. Further, biological tissue is collected into the outer cylinder member through the puncture needle and the tubular body by a suction operation of sliding the piston member outwardly of the outer cylinder member. Subsequently, the living tissue collected into the syringe is frozen by the freezing device of the tissue processing means.

It is preferable that the said freezing apparatus is equipped with the Peltier element attached to the said outer cylinder member of the said syringe, and the power supply apparatus which supplies electric power to this Peltier element.

In the above configuration, by supplying electric power from the power supply device to the Peltier element, the outer cylinder member of the syringe is cooled by the Peltier element to freeze the biological tissue collected into the syringe.

The insertion portion has a conductive elongated tubular body, the puncture needle is formed at the distal end of the tubular body, and the tissue collecting means includes an outer cylinder member detachably connected to a proximal end of the tubular body, and a cylinder of the outer cylinder member. A syringe having an axial piston member inserted slidably in the axial direction, and a tissue collecting portion disposed at the distal end of the tubular body, by a suction operation of sliding the piston member outwardly of the outer cylinder member. It is preferable to have a tissue collection mechanism which collects biological tissue into the said tissue collection part via the puncture needle, and the said tissue processing means has a freezing apparatus which freezes the biological tissue collected into the said tissue collection part.

And in the said structure, the outer cylinder member of the tissue collection means which is a syringe can be detachably connected to the base end of the elongate tubular body in which the puncture needle was formed in the front end part, and an axial piston member can slide in an axial direction in the cylinder of an outer cylinder member. Insert it. Further, the living tissue is collected by the tissue collection portion of the tip of the tubular body by a suction operation of sliding the piston member in the outward direction of the outer cylinder member. Subsequently, the living tissue collected into the tissue collecting unit is frozen by a freezing device of the tissue processing means.

It is preferable that the said tissue processing means has a detachment means which detach | desorbs the biological tissue frozen in the said freezing apparatus from the said endoscope treatment instrument.

The tissue processing means has a treatment member that performs analytical processing on the biological tissue collected by the tissue collection means, and the living body that performs the biomolecular analysis of the living tissue by bringing the treatment member into contact with the living tissue. It is preferable to have a molecular analysis part.

And in the said structure, the analysis process is performed by the treatment member of a tissue processing means to the biological tissue collected by the tissue collection means. At this time, the biological member is subjected to the biomolecular analysis of the biological tissue by bringing the treatment member into contact with the biological tissue.

Preferably, the tissue processing means has biological tissue preservation means capable of stably preserving biological molecules in the biological tissue while keeping the frozen biological tissue frozen in the freezing apparatus.

The biotissue preservation means is a preservation reagent capable of stably preserving the biomolecule in the frozen tissue and a low-temperature environment capable of preventing degradation of the biomolecule by inhibiting the activity of the degrading enzyme on the biomolecule. It is desirable to have a means to retain.

The tissue processing means includes a sensor needle protruding from the piston member of the syringe, and a biomolecular analysis in which biomolecular analysis is performed by puncturing the sensor needle on a biological tissue collected into the tissue collection unit. It is preferable to have a part.

In the above configuration, the sensor needle protruding from the piston member of the syringe is punctured by the biological tissue collected into the tissue collecting unit so that the biomolecular analysis is performed by the biomolecular analyzer connected to the sensor needle.

A biological tissue analysis processing system according to another aspect of the present invention includes tissue collecting means for sucking and collecting living tissue and tissue processing means for performing analytical processing on the biological tissue collected by the tissue collecting means. do.

In the above configuration, the living tissue is sucked and collected by the tissue collecting means. The biological tissue collected after that is subjected to analytical processing by a tissue processing means.

It is preferable that the said tissue processing means is a means which performs at least any one of freezing, freeze drying, splitting, crushing, ethanol fixation, and formalin fixation to the said living tissue.

A biological tissue analysis processing system according to another aspect of the present invention includes an endoscope for observing a living body and an endoscope treatment instrument inserted into the living body through a treatment instrument channel of the endoscope, and the endoscope treatment instrument is the treatment. Elongated insertion portion inserted into the living body through the spherical channel, tubular puncture needle disposed at the distal end of the insertion portion and inserted into the living tissue, and living tissue in the state where the puncturing needle is inserted into the living tissue And tissue processing means for performing analytical processing on the biological tissue collected by the tissue collecting means.

In the above configuration, the tubular puncture needle disposed at the distal end of the elongated insertion portion inserted into the living body through the treatment instrument channel of the endoscope for observing the living body is inserted into the living tissue. In the state where the puncture needle is inserted into the living tissue, the living tissue is collected by the tissue collecting means. The biological tissue collected after that is subjected to analytical processing by a tissue processing means.

The tissue collecting means has an outer cylinder member detachably connected to a proximal end of the tubular body, and an axial piston member inserted into the cylinder of the outer cylinder member so as to be slidable in an axial direction. And a syringe for collecting biological tissue into the outer cylinder member through the puncturing needle and the tubular body by a suction operation of sliding outwardly, and the tissue processing means includes a biological tissue collected into the syringe. It is preferable to have a freezing apparatus which freezes, The said freezing apparatus is equipped with the Peltier element attached to the said outer cylinder member of the said syringe, and the power supply apparatus which supplies electric power to this Peltier element.

And in the said structure, the outer cylinder member of the tissue collection means which is a syringe can be detachably connected to the base end of the elongate tubular body in which the puncture needle was formed in the front end part, and an axial piston member can slide in an axial direction in the cylinder of an outer cylinder member. Insert it. Further, biological tissue is collected into the outer cylinder member through the puncture needle and the tubular body by a suction operation of sliding the piston member outwardly of the outer cylinder member. Subsequently, electric power is supplied from the power supply device to the Peltier element, thereby cooling the outer cylinder member of the syringe by the Peltier element and freezing the biological tissue collected into the syringe.

The tissue collecting means has an outer cylinder member detachably connected to a proximal end of the tubular body, and an axial piston member inserted into the cylinder of the outer cylinder member so as to be slidable in an axial direction. And a syringe for collecting biological tissue into the outer cylinder member through the puncturing needle and the tubular body by a suction operation of sliding outwardly, and the tissue processing means includes: a biological tissue collected into the syringe. It is preferable to have the treatment member which performs the process for analysis, and has the biomolecular analyzer which performs the biomolecule analysis of the said biological tissue by making the said treatment member contact the said biological tissue.

And in the said structure, biological tissue is extract | collected inside the outer cylinder member by the suction operation which slides a piston member to the outer direction of an outer cylinder member. Subsequently, the biomolecular analysis of the living tissue is performed by bringing the treatment member of the tissue processing means into contact with the living tissue collected into the outer cylinder member.

According to another aspect of the present invention, there is provided a method for sampling a tissue analysis process, the method comprising: inserting an endoscope treatment instrument having a tubular puncture needle into a living body through a treatment instrument channel of an endoscope for observing the living body; A step of inserting the puncture needle into the biological tissue, a tissue collection step of collecting the biological tissue into the endoscope treatment instrument in the state where the puncture needle is inserted into the biological tissue, and the biological tissue collected by the tissue collecting step And a step of freezing the tissue for freezing for analysis, and extracting pellets of the frozen piece of biological tissue frozen in the tissue freezing process from the endoscope treatment tool and sending the pellets to a tissue analysis processing unit which is a later step. Characterized in that.

In the above configuration, the endoscope treatment instrument is inserted into the living body through the treatment instrument channel of the endoscope for observing the body (the treatment instrument insertion step), and the puncture needle is inserted into the living tissue (the puncture needle insertion process). Thereafter, in the state where the puncture needle is inserted into the living tissue, the living tissue is collected in the endoscope treatment instrument (tissue collecting step). Subsequently, freezing treatment for analysis is performed on the biological tissue collected by the tissue collecting step (tissue freezing treatment step). Thereafter, the pellets of the frozen piece of the biological tissue frozen in the tissue freezing treatment step are taken out from the endoscope treatment instrument and sent to a tissue analysis processing unit which is a post-process (transfer step).

According to the present invention, it is possible to provide an endoscope treatment instrument, a biological tissue analysis processing system, and a sample collection method for tissue analysis processing, which can keep the collected biological tissue fresh and can perform accurate diagnosis.

In addition, in the present invention, the biomolecule is a relatively high molecular molecule such as various molecules constituting the living body, that is, various proteins such as nucleic acids such as DNA and RNA in tissues, receptors, transcription factors, enzymes, lipids such as hormones, etc. It includes relatively small molecules such as metabolites in cells, or those derived from foreign organisms such as viruses infected with cells.

Generally, it is desirable to freeze these biomolecules instantaneously in order to stably preserve them without compromising their function. This is because degradation is prevented by inhibiting the activity of the degrading enzymes on various biomolecules originally contained in the cell under low temperature environment.

The temperature at this time becomes like this. Preferably it is 4 degrees C or less, More preferably, it is minus 20 degrees C or less, More preferably, minus 80 degrees C or less is preferable. Since the activity of DNAase is maintained at at least 4 ° C, preferably 0 ° C or less at which water contained in the tissue is frozen.

In addition, if frozen immediately, it is also very important to remain frozen until analysis is performed. For example, when transporting a biological sample or when the sample is kept at a high temperature or repeats freezing and thawing, the cells constituting the living tissue are destroyed and the structure and form of the cells are destroyed. At the same time, the breakdown enzymes of various biomolecules are released to rapidly break down the biomolecules. For this reason, the state of the biomolecule in the living tissue is considerably changed from the time point in which it was in the living body, and accurate analysis is impossible. Therefore, when the biological tissue is collected, it is important to freeze it immediately and then return it to the cryopreservation means or analysis means while maintaining the frozen state. Therefore, according to the present invention, it is possible to stably collect, store, and analyze the biological molecules in the biological tissues without damaging the state at the time of collecting the biological tissues.

Fig. 1A is a schematic configuration diagram of the entire endoscope with the endoscope treatment instrument in the first embodiment of the present invention.

1B is a plan view showing a distal end portion of the endoscope of the first embodiment.

Fig. 2 is a schematic configuration diagram showing the endoscope treatment instrument of the first embodiment.

Fig. 3 is a block diagram showing a schematic configuration of a control system for tissue freezing treatment of the endoscope treatment instrument of the first embodiment.

It is explanatory drawing for demonstrating the state which the pellet of the frozen piece of living tissue was taken out from the syringe of the endoscope treatment instrument of 1st Embodiment.

Fig. 5 is a flowchart for explaining a collection operation of living tissue by the endoscope treatment instrument of the first embodiment.

Fig. 6 is a schematic block diagram showing a second embodiment of the present invention.

It is a schematic block diagram which shows the endoscope treatment instrument of 3rd Embodiment of this invention.

FIG. 7B is a side view illustrating the metal mesh resin tube of the third embodiment. FIG.

Fig. 7C is a perspective view showing a storage case of living tissue of the third embodiment.

FIG. 8A is a schematic block diagram showing a state in which a living tissue frozen by the endoscope treatment instrument of the third embodiment is accommodated in an outer cylinder of a syringe together with a storage case. FIG.

FIG. 8B is a perspective view showing a state in which the living tissue frozen by the endoscope treatment instrument of the third embodiment is taken out of the syringe together with the case; FIG.

Fig. 9 is a flowchart for explaining a collecting operation of living tissue by the endoscope treatment instrument of the third embodiment.

Fig. 10 is a schematic configuration diagram of the entire endoscope with the endoscope treatment instrument according to the fourth embodiment of the present invention.

Fig. 11A is a longitudinal sectional view of an essential part showing a frozen state of biological tissue collected into a storage case of an endoscope treatment instrument according to the fourth embodiment.

Fig. 11B is a perspective view showing a storage case of living tissue of the fourth embodiment.

12 is a longitudinal sectional view of an essential part showing a state where a sensor needle is punctured in a living tissue frozen in a storage case of an endoscope treatment instrument according to a fourth embodiment.

It is a flowchart for demonstrating the collection work of a living tissue by the endoscope treatment instrument of 4th Embodiment.

Fig. 14 is a schematic configuration diagram of the entire endoscope with the endoscope treatment instrument in the fifth embodiment of the present invention.

15 is a schematic configuration diagram of a main part of the endoscope treatment instrument of the fifth embodiment.

Fig. 16 is a schematic configuration diagram of an essential part showing the open state of the filter of the endoscope treatment instrument according to the fifth embodiment.

Fig. 17 is a schematic structural diagram of the whole biological tissue analysis processing system of the sixth embodiment of the present invention.

Fig. 18 is a schematic structural diagram of the whole biological tissue analysis processing system of the seventh embodiment of the present invention.

19 is a flowchart for explaining a sampling process in the biological tissue analysis processing system according to the eighth embodiment of the present invention.

20 is a schematic block diagram for explaining the use of arraying by sampling processing in the eighth embodiment.

EMBODIMENT OF THE INVENTION Hereinafter, 1st Embodiment of this invention is described with reference to FIGS. 1A-5. Fig. 1A shows a schematic configuration of the entire flexible side view type electronic endoscope 2 with the endoscope treatment instrument 1 according to the present embodiment.

The electronic endoscope 2 has an elongate insertion portion 3. The base part of the insertion part 3 is connected with the operation part 4 of a thick width. The insertion part 3 is provided with the elongate flexible pipe part 5, the bendable bend part 6, and the front end structure part 7. And the base end of the flexible pipe part 5 is connected to the operation part 4. The front end of the curved operation wire (not shown) is connected to the curved portion 6.

As shown in FIG. 1B, the side hole 10a communicates with the observation window 8 of the observation optical system, the illumination window 9 of the illumination optical system, and the distal end of the treatment instrument channel 10 on the side surface of the tip constitution portion 7. ) Is arranged. In the observation window 8 of the observation optical system, the objective optical system is arranged behind the cover glass. An imaging device such as a CCD is disposed at an imaging position of the objective optical system. One end of the signal cable is connected to this imaging device. In the illumination window 9, the tip end part of a light guide fiber bundle is arrange | positioned behind the cover glass. In the side hole 10a, a forceps rising table is disposed.

In addition, the signal cable of the imaging element, the bundle of light guide fibers, the treatment instrument channel 10, the curved operation wire, and the like extend to the operation unit 4 side through the inside of the insertion unit 3. The signal cable of the imaging element, the bundle of light guide fibers, the treatment instrument channel 10, the bending operation wire, and the like are incorporated as an internal part in the insertion section 3.

The bending operation knob 11 and the treatment instrument insertion opening 12 are arrange | positioned at the operation part 4, and the one end part of the universal cord 13 is connected. The curved manipulation knob 11 is connected to a curved manipulation mechanism incorporated in the manipulation unit 4. The base end of the bending operation wire is connected to this bending operation mechanism. Then, by the operation of the bending operation knob 11, the bending operation wire is pulled through the bending operation mechanism so that the curved portion 6 is remotely operated in the operation direction of the bending operation knob 11.

The treatment instrument insertion port 12 is disposed at the front end portion of the operation unit 4. The treatment instrument insertion port 12 is connected to the proximal end of the treatment instrument channel 10. Then, the endoscope treatment instrument 1 of the present embodiment is inserted from the treatment instrument insertion port 12.

The connector 14 is connected to the other end of the universal cord 13. The connector portion 14 is connected with a proximal end of a connection cord for an electrical signal (not shown). An electrical connector is connected to the other end of the connection cord. The connector portion 14 is connected to a light source device and an electrical signal connector to a video processor, respectively. Here, the signal cable and the light guide fiber bundle are inserted into the universal cord 13 from the operation unit 4. The signal cable is connected to an electrical signal connector. In addition, the other end of the light guide fiber bundle is connected to the connector portion 14. The illumination light emitted from the light source device is focused on the incident end face of the light guide fiber bundle, and the illumination light is incident on the light guide fiber bundle.

Fig. 2 shows a schematic configuration of the endoscope treatment instrument 1 of the present embodiment. The endoscope treatment instrument 1 is provided with an elongated insertion tube (insertion portion) 15. This insertion tube 15 is formed of the resin tube (tubular body), for example. The insertion tube 15 is inserted into the living body through the treatment instrument channel 10 of the endoscope 2 to observe the living body. The tubular puncture needle 16 is disposed at the distal end of the insertion tube 15. The puncture needle 16 is formed with a sharp needle portion 16a that can be inserted into a living tissue.

At the proximal end of the insertion tube 15, a connection clamp 17 of an external mechanism is formed. On the inner circumferential surface of the coupling clamp 17, a tapered surface 17a is formed in which the opening area becomes larger toward the outside. A syringe (tissue collecting means) 18 is detachably connected to the connecting clamp 17 of the insertion tube 15. This syringe 18 has an outer cylinder member 19 and an axial piston member 20. A connecting end 21 having a thin diameter and a thin tip is formed at the tip end portion of the outer cylinder member 19. This connecting end 21 is connected in the state inserted in the connection clamp 17 of the insertion tube 15. As shown in FIG. At the base end of the outer cylinder member 19, a flange portion 19a for finger ring having a large diameter is formed. Moreover, it is preferable that the inner diameter dimension (effective diameter) of the outer cylinder member 19 of the syringe 18 is set as large as possible so that a large suction pressure can be exerted in the tubular body of the elongate insertion tube 15.

The piston member 20 is inserted in the cylinder of the outer cylinder member 19 so that sliding is possible in the axial direction. At the outer end of the piston member 20, a flange portion 20a for finger grips having a large diameter that prevents the inflow of the outer cylinder member 19 into the cylinder is formed. And the position where the piston member 20 was inserted in the outer cylinder member 19 in the state in which the puncture needle 16 of the endoscope treatment instrument 1 was stuck in the living tissue H (refer FIG. 7A). The living tissue H is transferred to the outer cylinder member 19 through the inside of the tubular body of the puncture needle 16 and the insertion tube 15 by a suction operation of sliding from the outer cylinder member 19 to the outer direction (upward in Fig. 2). Is collected inside).

In addition, the endoscope treatment instrument 1 of the present embodiment is subjected to analytical treatment to the biological tissue H collected by the syringe 18, and the biological tissue freezing to freeze the biological tissue H in the present embodiment. A freezing device (tissue processing means) 22 is provided. This freezing device 22 has a Peltier element 23 attached to the outer cylinder member 19 and a power supply device 24 for supplying electric power to the Peltier element 23.

3 is a block diagram showing a schematic configuration of a control system for tissue freezing processing incorporated in the power supply device 24. The power supply device 24 has, for example, a CPU 25 constituting a control means such as a computer. The CPU 25 is connected to a power supply unit 26, a switch 27, a temperature sensor 28, a Peltier element 23, a storage device (not shown), and the like. The switch 27 operates on / off the driving state of the Peltier element 23. In addition, the temperature sensor 28 measures the temperature of the biological tissue H picked up by the syringe 18.

The syringe 18 is also equipped with a heat transfer tube 29 formed of a metal material having high thermal conductivity or the like on the inner circumferential surface of the outer cylinder member 19. This heat transfer pipe 29 is provided in contact with the Peltier element 23. The temperature sensor 28 is provided in this heat exchanger tube 29.

Next, the operation of the above configuration will be described. Here, the operation at the time of use of the endoscope treatment instrument 1 of this embodiment is demonstrated according to the flowchart of FIG. First, the electronic endoscope 2 is inserted into the patient's body (step S1). At this time, the insertion portion 3 of the electronic endoscope 2 is inserted into the body from the tip configuration portion 7. During the insertion work of the endoscope 2, illumination light is irradiated from the illumination window 9 of the endoscope 2, and the body cavity inside is observed by the observation window 8. The endoscope image observed by the observation window 8 is displayed on a monitor (not shown).

In this state, the insertion part 3 of the endoscope 2 is inserted to the target site | part in a body cavity. After that, diagnosis of the target site in the body cavity is performed by the electronic endoscope 2 (step S2). At this time, the inspection target site in a body cavity, for example, a lesion part, is set. Thereafter, an operation of inserting the endoscope treatment instrument 1 into the living body through the treatment instrument channel 10 of the endoscope 2 is performed.

When the endoscope treatment instrument 1 is inserted, the insertion tube 15 of the endoscope treatment instrument 1 is inserted into the treatment instrument channel 10 from the treatment instrument insertion port 12 of the endoscope 2. Then, the insertion tube 15 of the endoscope treatment instrument 1 is inserted into the living body through the treatment instrument channel 10.

The insertion tube 15 of the endoscope treatment instrument 1 guided to the tip side through the treatment instrument channel 10 protrudes into the body from the side hole 10a. At this time, the protruding direction of the insertion tube 15 of the endoscope treatment instrument 1 is adjusted by the forceps weather station, and the protruding direction of the insertion tube 15 is remotely operated so as to face the desired direction.

Thereafter, the puncture needle 16 of the endoscope treatment instrument 1 is inserted into the suspicious examination target site set in the endoscope diagnosis at step S2 (step S3). At this time, a sample of the living tissue H1 at the test target site is taken into the tube of the puncture needle 16. In this state, the syringe 18 is operated. At this time, the piston member 20 is slid in the outward direction (upward in Fig. 2) of the outer cylinder member 19 from the position where the piston member 20 is inserted into the outer cylinder member 19. In this operation, a suction force for sucking the living tissue H through the inside of the tube of the puncture needle 16 and the insertion tube 15 acts. Therefore, by this suction operation, the biological tissue H at the inspection target site is collected inside the outer cylinder member 19 of the syringe 18 (step S4). In addition, the biological tissue H collected at this time contains blood and a mucous membrane.

Thereafter, the switch 27 of the power supply device 24 is turned on to drive the freezing device 22 (step S5). When the freezing device 22 is driven, electric power is supplied to the Peltier element 23, and the Peltier element 23 is driven (step S6). Thereby, the outer cylinder member 19 of the syringe 18 is cooled by the Peltier element 23 (step S7), and the biological tissue collected into the syringe 18 is frozen (step S8). At this time, the freezing temperature of the living tissue is set at, for example, about -10 ° C to -60 ° C. As the freezing material of the freezing device 22, for example, the freezing temperature of the living tissue can be set at about -80 ° C when dry ice is used and about -196 ° C when liquid nitrogen is used. The biological tissues collected into the syringe 18 may be treated with a toriton 100 surfactant as a cell membrane lysate to facilitate the analysis of the biological tissues.

In addition, after the biological tissue collected into the syringe 18 is frozen, the syringe 18 is detached from the connecting clamp 17 of the insertion tube 15. In this state, as shown in FIG. 4, the piston member 20 of the syringe 18 is pushed out and the pellet P of the living tissue frozen in the outer cylinder member 19 is taken out of the syringe 18. As shown in FIG. (Step S9). Thereafter, the pellet P of the frozen biological tissue is sent to a tissue analysis processing unit such as a biomolecular analyzer which is a later step (step S10). Thereby, the sample collection operation | work for a tissue analysis process is complete | finished.

Therefore, the following effects are exhibited in the thing of the said structure. That is, in the endoscope treatment instrument 1 of the present embodiment, the insertion tube 15 is inserted into the living body through the treatment instrument channel 10 of the endoscope 2, and the puncture needle (at the distal end of the insertion tube 15) ( In the state where 16) was inserted into the living tissue, the living tissue was aspirated and collected by the syringe 18, and then the collected living tissue was frozen by the freezing device 22, so that the analysis was performed. One biological tissue can be kept fresh. Therefore, the biological tissue can be sent to a tissue analysis processing unit such as a biomolecular analyzer, which is a post-process, as it is, so that various tests of the biological tissue, for example, cytoplasm, tissue, and biomolecule analysis, can be performed with high accuracy.

In addition, in this embodiment, although the structure which inserts the endoscope treatment instrument 1 into the body through the treatment instrument channel 10 of the flexible side view type electronic endoscope 2 was demonstrated, it is not limited to this. For example, the configuration may be inserted into the body through the treatment instrument channel of the flexible direct-view electronic endoscope 2.

6 shows a second embodiment of the present invention. In this embodiment, the endoscope treatment instrument 1 described in the first embodiment (see FIGS. 1A to 5) is inserted into the body through the treatment instrument channel 32 of the hard mirror 31. In addition, since the structure of the endoscope treatment instrument 1 is the same structure as 1st Embodiment, the same code | symbol is attached | subjected to the same part as 1st Embodiment, and the description is abbreviate | omitted.

The hard mirror 31 of this embodiment is provided with the elongate hard insertion part 33 of the straight tube shape formed from the metal pipe, for example. An operation part 34 having a large diameter is provided at the proximal end of the insertion part 33. The observation window 35 of the observation optical system, the illumination window 36 of the illumination optical system, and the tip opening 32a of the treatment instrument channel 32 are formed at the tip of the insertion part 33.

Moreover, the eyepiece 37 for observation and the light guide connection clamp 38 protrude from the outer peripheral surface of the operation part 34. An image transmission optical system is arranged between the eyepiece 37 and the observation window 35 to transmit the observation image incident from the observation window 35 to the eyepiece 37. In addition, a light guide for transmitting illumination light is disposed between the light guide connecting clamp 38 and the illumination window 36.

The rear end opening 32b of the treatment instrument channel 32 is formed in the terminal portion of the operation portion 34. The insertion tube 15 of the endoscope treatment instrument 1 is inserted into the treatment instrument channel 32 from the rear end opening 32b and is inserted into the living body through the treatment instrument channel 32.

Next, the effect | action of this embodiment of the said structure is demonstrated. When using the endoscope treatment instrument 1 of this embodiment, the hard mirror 31 is previously inserted in the body of a patient. When inserting the hard mirror 31, for example, a trocar is used. The troker has a coat tube and a puncture needle inserted into the coat tube to be inserted into the coat tube. Then, in the state where the puncture needle is inserted and attached to the coat tube, it is stuck into the abdominal wall of the patient, for example. Thereafter, in the state where the puncture needle is removed from the inside of the outer tube, only the outer tube is inserted into the abdominal wall portion. Then, the hard mirror 31 is inserted into the patient's body through the outer canal.

After insertion of the hard mirror 31, the insertion tube 15 of the endoscope treatment instrument 1 is inserted into the living body through the treatment instrument channel 32 of the hard mirror 31. Thereafter, the biological tissue H is collected by the endoscope treatment instrument 1 by the same operation as in the first embodiment. Then, after the biological tissue collected into the syringe 18 is frozen, the piston member 20 of the syringe 18 is pushed out so that the pellet P of the biological tissue frozen in the outer cylinder member 19 is syringed ( The work taken out of 18) is performed similarly to the first embodiment.

Therefore, also in the present embodiment, the insertion tube 15 of the endoscope treatment instrument 1 is inserted into the living body through the treatment instrument channel 32 of the hard mirror 31, and the puncture needle of the distal end portion of the insertion tube 15 ( In the state where 16) is inserted into the living tissue, the living tissue is suction-collected by the syringe 18, and then the collected living tissue is frozen by the freezing device 22, and the analysis process can be performed. Therefore, similarly to the first embodiment, the collected biological tissue can be kept in a fresh state, and thus the biological tissue can be sent to a tissue analysis processing unit such as a biomolecular analysis device which is a post-process as it is in the fresh state. As a result, various tests of living tissues, for example, cytology, tissue photography, biomolecule analysis and the like can be performed with high accuracy.

7A to 9 show a third embodiment of the present invention. Fig. 7A shows a schematic configuration of the endoscope treatment instrument 41 of this embodiment. The endoscope treatment instrument 41 is provided with an elongated insertion tube (insertion portion) 42. As shown in Fig. 7B, the insertion tube 42 is, for example, attached to a metal mesh resin tube provided with a metal mesh 44 braided with a metal wire in a lattice shape on the outer circumferential surface of the resin tube (tube) 43. It is formed by. The resin tube 43 is formed of a material with high thermal conductivity. The metal mesh 44 may be integrally molded in a state where the metal mesh 44 is embedded in the resin tube 43. The outer surface of the insertion tube 42 is coated with, for example, a silicone coat, and the metal mesh 44 is prevented from being exposed to the outer surface of the insertion tube 42.

The insertion tube 42 is an endoscope for observing the living body, for example, the treatment instrument channel 10 of the flexible side view type electron endoscope 2 of the first embodiment (see FIGS. 1A to 5), or the second. 6 is inserted into the living body through the treatment instrument channel 32 of the hard mirror 31 of the embodiment (see FIG. 6). The tubular puncture needle 45 is disposed at the distal end of the insertion tube 42. The puncture needle 45 is formed with a sharp needle portion 45a that can be inserted into a living tissue.

At the base end of the insertion tube 42, a connection clamp 46 of an external mechanism is formed. On the inner circumferential surface of this connecting clamp 46, a tapered surface 46a is formed in which the opening area increases toward the outside. The syringe 47 is detachably connected to the connection clamp 46 of the insertion tube 42. This syringe 47 has an outer cylinder member 48 and an axial piston member 49. A connecting end 50 having a thin diameter and a thin tip is formed at the tip end of the outer cylinder member 48. This connecting end 50 is connected in the state inserted into the connection clamp 46 of the insertion tube 42. As shown in FIG. At the base end of the outer cylinder member 48, a flange portion 48a for finger ring having a large diameter is formed. In addition, it is preferable that the inner diameter dimension (effective diameter) of the outer cylinder member 48 of the syringe 47 is set as large as possible so that a large suction pressure can be applied to the tubular body of the elongated insertion tube 42.

The piston member 49 is inserted in the cylinder of the outer cylinder member 48 so that sliding is possible in the axial direction. At the outer end of the piston member 49, a flange portion 49a for finger grips having a large diameter that prevents the outer cylinder member 48 from entering the cylinder is formed.

Moreover, the endoscope treatment instrument 41 of this embodiment is set in the state which inserted the biological tissue storage case 51 made of resin with high thermal conductivity in the front-end | tip part of the insertion tube 42. As shown in FIG. This living tissue storage case 51 is a cylindrical case having a bottom where one end side of the cylindrical body is closed, as shown in Fig. 7C. It is preferable that this storage case 51 can be sliced. On the open end side of the storage case 51, an inclined surface open end portion 51a, which is obliquely cut out to fit the shape of the puncture needle 45 at the tip of the insertion tube 42, is formed. In the state where the storage case 51 is set in the insertion tube 42, the bottom side of the storage case 51 is inserted into the insertion tube 42 (arranged in the upper side in FIG. 7A), and the opening end is provided. 51a is positioned in the state which overlapped with the front-end opening of the puncture needle 45, mutually.

In addition, the Peltier element 52 is attached to the connection clamp 46 of the insertion tube 42. The Peltier element 52 is connected with a connection cord 54 with a power supply device 53 for supplying electric power. Then, driving power is supplied from the power supply device 53 to the Peltier element 52 through the connection cord 54. At the time of driving this Peltier element 52, the metal mesh 44 of the insertion tube 42 is cooled, and the living case 51 is cooled by heat conduction, and the biological tissue collected in the storage case 51 ( H1) is intended to freeze. Then, the biological tissue H collected in the storage case 51 by the Peltier element 52 and the power supply device 53 is subjected to analysis for treatment, and in this embodiment, the biological tissue freezing that freezes the biological tissue H. A freezing device (tissue processing means) 55 is formed.

In addition, the power supply device 53 is provided with a switch 56 for turning on / off a driving state of the Peltier element 52. This switch 56 has, for example, an operation dial 57 for selectively switching between any of the off (stop) position 56a, the weak position 56b, and the strong position 56c. And when the operation dial 57 is set to the off (stop) position 56a, when it is set to a thawed state and about the position 56b, biological tissue H1 is, for example, -10 degreeC--30 In the case of being set in a weakly frozen state at a freezing temperature of about ° C and a strong position 56c, the biological tissues H are selectively switched to, for example, a cold freezing state at a freezing temperature of about -60 ° C to -80 ° C. It is supposed to be operated.

In addition, the power supply device 53 is provided with a control system (see FIG. 3) for tissue freezing treatment having the same configuration as in the first embodiment. As described above, the driving state of the Peltier element 52 can be selectively switched to the thawing state, the weak freezing state, and the strong freezing state according to the operation of the operation dial 57 of the switch 56. It is supposed to be done.

In addition, in the endoscope treatment instrument 41 of the present embodiment, the syringe 47 is used for the recovery of the biological tissue H collected into the storage case 51. That is, when the puncture needle 45 at the tip of the insertion tube 42 is inserted into the internal wall (biological tissue) H of an organ such as the stomach, as shown in Fig. 7A, the storage case ( 51) The biological tissue (H1) is collected into. At this time, the piston member 49 of the syringe 47 is held in the pushed position pushed into the outer cylinder member 48. After the living body tissue H1 is frozen, the outer cylinder member 48 is directed outward from the position where the piston member 49 of the syringe 47 is inserted into the outer cylinder member 48, as shown in Fig. 8A. The living body tissue H, which has been frozen through the inside of the tube of the puncture needle 45 and the insertion tube 42 by the suction action of sliding upward (in the upward direction in FIG. 7), together with the storage case 51, the syringe 47 It is sucked into the outer cylinder member 48 of and collect | recovered.

Next, the operation of the above configuration will be described. Here, the operation at the time of use of the endoscope treatment instrument 41 of this embodiment is demonstrated according to the flowchart of FIG. First, as in the first embodiment, the electronic endoscope 2 is inserted into the body of the patient (step S11). During the insertion work of the endoscope 2, illumination light is irradiated from the illumination window 9 of the endoscope 2 and the body cavity inside is observed by the observation window 8. The endoscope image observed by the observation window 8 is displayed on a monitor (not shown).

In this state, the insertion part 3 of the endoscope 2 is inserted to the target site | part in a body cavity. After that, diagnosis of the target site in the body cavity is performed by the electronic endoscope 2 (step S12). At this time, the inspection target site in a body cavity, for example, a lesion part, is set. Thereafter, an operation of inserting the endoscope treatment instrument 41 into the living body through the treatment instrument channel 10 of the endoscope 2 is performed.

When the endoscope treatment instrument 41 is inserted, the insertion tube 42 of the endoscope treatment instrument 41 is inserted into the treatment instrument channel 10 from the treatment instrument insertion port 12 of the endoscope 2. Then, the insertion tube 42 of the endoscope treatment instrument 41 is inserted into the living body through the treatment instrument channel 10.

The insertion tube 42 of the endoscope treatment instrument 41 guided to the tip side through the treatment instrument channel 10 protrudes into the body from the side hole 10a. At this time, the protruding direction of the insertion tube 42 of the endoscope treatment instrument 41 is adjusted by the forceps weather station, and the protruding direction of the insertion tube 42 is remotely operated so as to face the desired direction.

Thereafter, the puncture needle 45 of the endoscope treatment instrument 41 is inserted into the suspicious inspection target site set in the endoscope diagnosis in step S12 (step S13). At this time, when the puncture needle 45 of the endoscope treatment instrument 41 is inserted into the internal wall (living tissue) H of an organ such as the stomach, for example, the living tissue ( H) is taken.

In this state, the operation dial 57 of the switch 56 of the power supply device 53 is subsequently operated. At this time, the operation dial 57 is switched to either the weak position 56b or the strong position 56c to drive the power supply device 53 (step S14).

At the time of driving this power supply 53, electric power is supplied to the Peltier element 52 according to the operation of the operation dial 57, and the Peltier element 52 is driven (step S15). At the time of driving this Peltier element 52, the metal mesh 44 of the insertion tube 42 is cooled (step S16). Moreover, the storage case 51 is cooled by heat conduction (step S17). Thereby, the living tissue H1 collected into the storage case 51 is frozen (step S18). At this time, when the operation dial 57 of the power supply device 53 is set at the medicine position 56b, the living tissue H is weakly frozen at a freezing temperature of, for example, about -10 ° C to -30 ° C. , When set at the strong position 56c, the living tissue H is frozen in a strong freezing state at a freezing temperature of, for example, about -60 ° C to -80 ° C. Also in this case, the living tissue collected into the storage case 51 may be treated with a toriton 100 (registered trademark) surfactant as a cell membrane lysate so as to easily analyze the living tissue.

Furthermore, after freezing the biological tissue collected into the storage case 51, the syringe 47 is operated. At this time, the piston member 49 of the syringe 47 is held in the pushed position pushed into the outer cylinder member 48 as shown in Fig. 7A. After the living body tissue H is frozen, the piston member 49 of the syringe 47 is slid from the inside of the outer cylinder member 48 to the outer direction of the outer cylinder member 48 as shown in FIG. 8A. A suction operation is performed. The living tissue H1 frozen by this suction operation is sucked into the outer cylinder member 48 of the syringe 47 through the inside of the tube body of the puncture needle 45 and the insertion tube 42 together with the storage case 51. (Step S19).

Thereafter, the syringe 47 is detached from the connecting clamp 46 of the insertion tube 42. In this state, the piston member 49 of the syringe 47 is pushed out and the pellet P of the frozen biological tissue H1 is taken out of the syringe 47 together with the storage case 51. Thereafter, as shown in Fig. 8B, the pellet P of the frozen biological tissue stored in the storage case 51 is discharged from the storage case 51 and taken out (step S20). Subsequently, the pellet P of the frozen biological tissue is sent to a tissue analysis processing unit such as a biomolecular analyzer which is a later step (step S21). As a result, the sampling operation for tissue analysis processing is terminated.

Therefore, the following effect is exhibited in the thing of the said structure. That is, in the endoscope treatment instrument 41 of the present embodiment, the insertion tube 42 is inserted into the living body through the treatment instrument channel 10 of the endoscope 2, and the puncture needle (at the distal end of the insertion tube 42) ( By inserting 45 into the living tissue, the living tissue is collected into the storage case 51. After that, the collected biological tissue H1 is frozen by the freezing device 55, and the analysis process is performed. Therefore, the collected biological tissue H1 can be kept fresh. Therefore, the living tissue H1 can be sent to a tissue analysis processing unit such as a biomolecular analyzer, which is a post-process as it is, so that various tests of the living tissue H1, for example, cytogene, tissue, and biomolecule analysis can be performed. It can be performed with high precision.

10 to 13 show a fourth embodiment of the present invention. In the endoscope treatment instrument 61 of the present embodiment, the biomolecular analyzer 62 is added to the endoscope treatment instrument 1 of the first embodiment (see FIGS. 1A to 5). In addition, the part other than this is the same structure as the endoscope treatment instrument 1 of 1st Embodiment, and attaches | subjects the same code | symbol to the same part as the endoscope treatment instrument 1 of 1st Embodiment, and is demonstrated here. Omit.

FIG. 10 is a schematic block diagram showing a state where the endoscope treatment instrument 61 of the present embodiment is inserted into the treatment instrument channel 10 of the electronic endoscope 2. 11A shows the schematic structure of the whole endoscope treatment instrument 61 of this embodiment.

In the endoscope treatment instrument 61 of the present embodiment, a biomolecular analysis sensor 63 is incorporated in the piston member 20 of the syringe 18. The proximal end of the sensor needle 64 protruding forward from the distal end (inner end) of the piston member 20 is connected to the biomolecular analysis sensor 63. This sensor needle 64 is arrange | positioned at the axial center position of the front end surface of the piston member 20. As shown in FIG.

In addition, a storage case 51 substantially the same as the biological tissue storage case 51 of the third embodiment (see FIGS. 7A to 9) is inserted into the tubular puncture needle 16 at the tip of the insertion tube 15. It is set in the state. As shown in Fig. 11B, a through hole 51c is formed in the bottom portion 51b of the storage case 51 at the center position. The sensor needle 64 can be inserted into the through hole 51c of the storage case 51.

In addition, an external biomolecule analyzer 62 is connected to the biomolecule analysis sensor 63. This biomolecular analyzer 62 has a power switch 65, a data print switch 66, and a data printer 67.

Next, the operation of the above configuration will be described. Here, the operation at the time of use of the endoscope treatment instrument 61 of the present embodiment will be described with reference to the flowchart of FIG. First, similarly to the first embodiment, the electronic endoscope 2 is inserted into the body of the patient (step S31). During the insertion work of the endoscope 2, illumination light is irradiated from the illumination window 9 of the endoscope 2 and the body cavity inside is observed by the observation window 8. The endoscope image observed by the observation window 8 is displayed on a monitor (not shown).

In this state, the insertion part 3 of the endoscope 2 is inserted to the target site | part in a body cavity. Thereafter, the electronic endoscope 2 diagnoses the target site in the body cavity (step S32). At this time, the inspection target site in a body cavity, for example, a lesion part, is set. Thereafter, an operation of inserting the endoscope treatment instrument 61 into the living body through the treatment instrument channel 10 of the endoscope 2 is performed.

When the endoscope treatment instrument 61 is inserted, the insertion tube 15 of the endoscope treatment instrument 61 is inserted into the treatment instrument channel 10 from the treatment instrument insertion port 12 of the endoscope 2. Then, the insertion tube 15 of the endoscope treatment instrument 61 is inserted into the living body through the treatment instrument channel 10.

The insertion tube 15 of the endoscope treatment instrument 61 guided to the tip side through the treatment instrument channel 10 protrudes into the body from the side hole 10a. At this time, the protruding direction of the insertion tube 15 of the endoscope treatment instrument 41 is adjusted by the forceps weather station, and the protruding direction of the insertion tube 15 is remotely operated so as to face the desired direction.

Thereafter, the puncture needle 16 of the endoscope treatment instrument 61 is inserted into the suspicious inspection target site set in the endoscope diagnosis in step S32 (step S33). At this time, when the puncture needle 16 of the endoscope treatment instrument 61 is inserted into the internal wall (biological tissue) H of an organ such as the stomach as shown in Fig. 11A, the storage case. The biological tissue H1 is collected into 51. At this time, the piston member 20 of the syringe 18 is held in the pushed position pushed into the outer cylinder member 19.

In this state, a suction operation for slidably moving the piston member 20 of the syringe 18 from the inside of the outer cylinder member 19 to the outer direction of the outer cylinder member 19 as shown in FIG. 12 is performed. . By this suction operation, the living tissue H1 is sucked into the outer cylinder member 19 of the syringe 18 together with the storage case 51 through the inner tube of the insertion tube 15 (step S34). At this time, the sensor needle 64 is inserted into the through-hole 51c of the storage case 51, and the sensor needle 64 is inserted through the living tissue H1 in the storage case 51 (step S35).

Thereafter, the operation dial 57 of the switch 56 of the power supply device 53 is operated as necessary. At this time, the operation dial 57 is switched to either the weak position 56b or the strong position 56c to drive the power supply 53.

When the power supply device 53 is driven, electric power is supplied to the Peltier element 23 according to the operation of the operation dial 57, and the Peltier element 23 is driven. When the Peltier element 23 is driven, the biological tissue H1 collected into the storage case 51 is frozen (step S36).

Thereafter, the power switch 65 of the biomolecular analyzer 62 is turned on (step S37). At this time, the biomolecular analysis of the pellet P of the frozen biological tissue H1 stored in the storage case 51 by the sensor needle 64 of the biomolecular analysis sensor 63, for example, a specific protein. The presence or absence, the interaction, and the like are measured (step S38).

In addition, after the measurement of step S38, the data printer 67 of the biomolecule analysis device 62 is driven, and the information analysis of the biomolecule analysis device 62 is performed (step S39). In this information analysis, information, such as the possibility of metastasis (preliminary diagnosis after surgery), the optimization of a treatment policy, and anticancer drug sensitivity, is analyzed, for example. As a result, the tissue analysis processing operation is completed.

Therefore, the following effect is exhibited in the thing of the said structure. That is, in the endoscope treatment instrument 61 of the present embodiment, the insertion tube 15 is inserted into the living body through the treatment instrument channel 10 of the endoscope 2, and the puncture needle (at the distal end of the insertion tube 15) ( By inserting 16) into the living tissue, the living tissue is collected into the storage case 51. Then, by inserting the sensor needle 64 of the biomolecular analysis sensor 63 into the collected biological tissue H1, the biological molecules of the pellets P of the biological tissue H1 stored in the storage case 51. Interpretation, for example, the presence or absence of a specific protein and measurement of interaction, can be performed. Therefore, the biological tissue H1 can be subjected to post-process biomolecule analysis in a short time as it is in a fresh state, so that various tests of the biological tissue H1 can be performed with high accuracy, for example, cytology, tissue formation, and biomolecule analysis. have.

14 to 16 show a fifth embodiment of the present invention. This embodiment provides the endoscope treatment instrument 71 of a different structure from the endoscope treatment instrument 1 of 1st Embodiment (refer FIG. 1A-FIG. 5).

In the endoscope treatment instrument 71 of the present embodiment, a suction device 73 is provided in place of the syringe 18 of the first embodiment as a suction means for collecting biological tissue. In addition, the part other than this is the same structure as the endoscope treatment instrument 1 of 1st Embodiment, and attaches | subjects the same code | symbol to the same part as the endoscope treatment instrument 1 of 1st Embodiment, and is demonstrated here. Omit.

That is, the distal end portion of the suction pipe passage 72 for suction is connected to the proximal end of the insertion tube 15 to the endoscope treatment instrument 71 of the present embodiment. A suction device 73 is connected to the proximal end of the suction pipe passage 72. The suction device 73 is provided with a switch 74 for on / off operation. A suction probe is formed by the insertion tube 15 and the suction pipe passage 72.

In the vicinity of the connecting portion of the suction pipe line 72 with the suction device 73, a filter 75 for sampling is interposed. As shown in Fig. 15, the filter 75 is provided with a cylindrical filter container 76 having a bottom. The transparent cap 77 is detachably connected to the opening face of this filter container 76.

Moreover, inside the filter container 76, as shown in FIG. 16, the partition plate 78 of the mesh shape which forms a filter main body is arrange | positioned. The interior of the filter container 76 is partitioned by the partition plate 78 into a suction chamber on the suction device 73 side (bottom side) and a sampling chamber on the insertion tube 15 side (opening surface side). And the biological tissue H1 of the sample attracted in the inside of the filter container 76 is trapped by the partition plate 78, and is taken into a sample collection chamber.

In addition, the peripheral wall part of the filter container 76 is formed by the Peltier element built-in ring 79. The Peltier element built-in ring 79 is connected to a tissue cooling device 80 in which a power supply device for supplying electric power to the Peltier element is incorporated. The tissue cooling device 80 is provided with a switch 81 for on / off operation. When the switch 81 is turned on, electric power is supplied to and driven by the Peltier element of the Peltier element built-in ring 79, and the living tissue H1 of the sample taken into the filter container 76 is embedded with the Peltier element. It is supposed to be frozen by the ring 79.

Next, the operation of the above configuration will be described. In the endoscope treatment instrument 71 of the present embodiment, the endoscope treatment instrument in vivo through the treatment instrument channel 10 of the endoscope 2 in the same procedure as the endoscope treatment instrument 1 of the first embodiment. The operation of inserting the insertion tube 15 of 71 is performed.

Thereafter, the puncture needle 16 of the endoscope treatment instrument 71 is inserted into the suspected test target site of the lesion set for endoscopy. At this time, the sample of the biological tissue H1 of a test target site | part is taken in the tube of the puncture needle 16. FIG. In this state, the switch 74 of the suction device 73 is turned on to operate the suction force on the insertion tube 15 and the suction pipe passage 72. Thereby, the sample of the biological tissue H1 of the test target site | part collected into the tube of the puncture needle 16 is sucked in the inside of the filter container 76 via the insertion tube 15 and the suction line 72. As shown in FIG.

In addition, the biological tissue H1 of the sample sucked into the filter vessel 76 is trapped by the partition plate 78 and collected into the sample collection chamber. In this state, the switch 81 of the tissue cooling device 80 is turned on. Thereby, electric power is supplied and driven to the Peltier element of the Peltier element built-in ring 79, and the biological tissue H1 of the sample taken into the filter container 76 is frozen by the Peltier element embedded ring 79. . In addition, when the suction device 73 is driven, the tissue cooling device 80 is simultaneously driven so that the biological tissue H1 of the sample sucked inside the filter container 76 is trapped by the partition plate 78. It may be configured to freeze immediately.

In addition, after the biological tissue H1 collected into the filter container 76 is frozen, the transparent cap 77 is removed from the filter container 76. In this state, the frozen biological tissue H1 is taken out of the filter container 76. Thereafter, the frozen biological tissue H1 is sent to a tissue analyzing processing unit such as a biomolecule analyzing apparatus which is a post-process. Thereby, the sample collection operation | work for a tissue analysis process is complete | finished.

Therefore, the following effect is exhibited in the thing of the said structure. That is, in the endoscope treatment instrument 71 of the present embodiment, the insertion tube 15 is inserted into the living body through the treatment instrument channel 10 of the endoscope 2, and the puncture needle (the puncture end portion of the insertion tube 15) ( 16) is inserted into the living tissue, and then a suction force is applied to the insertion tube 15 and the suction pipe passage 72 by the suction device 73 to collect the living tissue H1 into the filter container 76. After that, the collected biological tissue H1 is frozen by the Peltier element built-in ring 79 and subjected to analytical processing, so that the collected biological tissue can be kept fresh. Therefore, the biological tissue can be sent to a tissue analysis processing unit such as a biomolecular analyzer, which is a post-process, as it is, so that various tests of the biological tissue, for example, cytoplasm, tissue, and biomolecule analysis, can be performed with high accuracy.

17 shows a sixth embodiment of the present invention. This embodiment applies the present invention to a laparoscopic surgical operation system. In Fig. 17, reference numeral 91 is a laparoscopic hard mirror, and 92 is a treatment instrument for collecting living tissue.

The hard mirror 91 is provided with the elongate hard insertion part 93 of the straight tube shape formed from the metal pipe, for example. At the proximal end of the insertion portion 93, a large operating portion 94 is provided. The observation window 95 of the observation optical system and the illumination window 96 of the illumination optical system are formed at the distal end of the insertion portion 93. Behind the observation window 95, an objective lens and an imaging element such as a CCD arranged at an imaging position of the objective lens are arranged. Behind the illumination window 96, a light guide for transmitting illumination light is arranged.

Moreover, one end of the universal cord 97 is connected to the outer peripheral surface of the operation part 94. The other end of the universal cord 97 is connected to a light source device (not shown), a connector portion connected to a video processor, or the like.

The treatment instrument 92 for biological tissue collection is provided with an elongated insertion tube (insertion section) 98. The insertion tube 98 is inserted into the living body through a coat tube of an unillustrated trocker previously inserted into the living tissue H2 of the abdominal wall part of the patient. A tubular puncture needle 99 is disposed at the distal end of the insertion tube 98. The puncture needle 99 is formed with a sharp needle portion 99a to the extent that the puncture needle 99 can be inserted into a target living tissue H3 such as a tumor.

At the proximal end of the insertion tube 98, a connection clamp 100 of an external mechanism is formed. On the inner circumferential surface of the connecting clamp 100, a tapered surface 100a is formed in which the opening area increases toward the outside. A syringe (tissue collecting means) 101 is detachably connected to the connecting clamp 100 of the insertion tube 98. This syringe 101 has an outer cylinder member 102 and an axial piston member 103. A connecting end 104 having a thin diameter and a thin tip is formed at the tip end of the outer cylinder member 102. This connection end 104 is connected in the state inserted into the connection clamp 100 of the insertion tube 98. As shown in FIG. At the base end of the outer cylinder member 102, a flange portion 102a for finger grip having a large diameter is formed. In addition, it is preferable that the inner diameter dimension (effective diameter) of the outer cylinder member 102 of the syringe 101 is set as large as possible so that a large suction pressure can be applied to the tubular body of the elongated insertion tube 98.

The piston member 103 is inserted in the cylinder of the outer cylinder member 102 so that sliding is possible in the axial direction. At the outer end of the piston member 103, a flange portion 103a for finger grips having a large diameter is formed to prevent intrusion of the outer cylinder member 102 into the cylinder. And the outer cylinder member from the position which inserted the piston member 103 into the outer cylinder member 102 in the state which the puncture needle 99 of the insertion tube 98 stuck in the living tissue H3 in the patient's body. A sample H3a of a living tissue H3 such as a tumor through the inside of the tube of the puncture needle 99 and the insertion tube 98 by a suction action of sliding in the outward direction (upward in Fig. 17) of the 102. Is collected inside the outer cylinder member 102.

In addition, the treatment instrument 92 for biological tissue collection of the present embodiment carries out an analysis process on the sample H3a of the biological tissue H3 collected by the syringe 101, and in this embodiment, the biological tissue H3. The freezing apparatus (tissue processing means) 105 which performs the living body freezing which freezes the sample H3a of this is provided. This freezing device 105 has a Peltier element 106 attached to the outer cylinder member 102 as in the first embodiment, and a power supply device 107 that supplies electric power to the Peltier element 106.

Next, the operation of the above configuration will be described. In the present embodiment, the insertion portion 93 of the hard mirror 91 is inserted into the body through the inside of the outer tube of the troker (not shown) which has been previously inserted into the living tissue H2 of the abdominal wall portion of the patient. Similarly, the insertion tube 98 of the treatment instrument 92 for collecting biological tissue is inserted into the living body through a coat tube of an unillustrated trocker previously inserted into the living tissue H2 of the abdominal wall part of the patient. And the operation | work which the puncture needle 99 of the front-end | tip part of the insertion tube 98 sticks in the target biological tissue H3, such as a tumor, is performed in the observation visual field by the hard mirror 91. At this time, the sample H3a of the biological tissue H3 of the test target site is taken in the tube of the puncture needle 99. In this state, the syringe 101 is operated. At this time, the piston member 103 is slid in the outward direction (upward in FIG. 17) of the outer cylinder member 102 from the position where the piston member 103 is inserted into the outer cylinder member 102. In this operation, a suction force for sucking the sample H3a of the living tissue H3 through the tube body of the puncture needle 99 and the insertion tube 98 acts. Therefore, as shown in FIG. 17, the sample H3a of the biological tissue H3 of the inspection target site is taken into the inside of the outer cylinder member 102 of the syringe 101 by this suction operation.

Thereafter, the switch 108 of the power supply device 107 is turned on to drive the freezing device 105. When the freezing device 105 is driven, electric power is supplied to the Peltier element 106 to drive the Peltier element 106. Thereby, the outer cylinder member 102 of the syringe 101 is cooled by the Peltier element 106, and the sample H3a of the living tissue H3 collected into the syringe 101 is frozen.

In addition, after the sample H3a of the biological tissue H3 collected into the syringe 101 is frozen, the syringe 101 is detached from the connecting clamp 100 of the insertion tube 98. In this state, the piston member 103 of the syringe 101 is pushed out so that the pellet P of the sample H3a of the living tissue H3 frozen in the outer cylinder member 102 is out of the syringe 101. Take out. Thereafter, the pellet P of the sample H3a of the frozen biological tissue H3 is sent to a tissue analysis processing unit such as a biomolecular analyzer which is a later step. Thereby, the sample collection operation | work for a tissue analysis process is complete | finished.

Therefore, the following effect is exhibited in the thing of the said structure. That is, in the laparoscopic surgical operation system of the present embodiment, the puncture needle 99 of the distal end portion of the insertion tube 98 of the treatment instrument 92 for collecting the biological tissue within the observation field of the hard mirror 91 is removed. After aspirating the sample H3a of the living tissue H3 with the syringe 101 in the state inserted into the living tissue H3, the collected living tissue is frozen by the freezing device 105 for analysis. Since the treatment is performed, the sample H3a of the collected biological tissue H3 can be kept fresh. Therefore, the sample H3a of the living tissue H3 can be sent to a tissue analysis processing unit such as a biomolecular analysis device which is a post-process as it is in the fresh state. Therefore, various tests of the sample H3a of the living tissue H3, for example Cell lines, tissue lines, biomolecule analysis and the like can be performed with high accuracy.

18 shows a seventh embodiment of the present invention. This embodiment installs the biological tissue analysis processing system 111 different from each embodiment mentioned above. In the biological tissue analysis processing system 111 of this embodiment, the syringe 112 is mainly used. This syringe 112 has an outer cylinder member 113 and an axial piston member 114. At the tip end of the outer cylinder member 113, a connecting end (number missing) having a thin diameter and a thin tip is formed. The proximal end of the tubular injection needle 115 is fixed to this connecting end?. At the base end of the outer cylinder member 113, a flange portion 113a for finger grip having a large diameter is formed.

The piston member 114 is inserted in the cylinder of the outer cylinder member 113 so that sliding is possible in the axial direction. At the outer end of the piston member 114, a flange portion 114a for finger grips having a large diameter which prevents the outer cylinder member 113 from entering the cylinder is formed. When the syringe 112 is in use, the piston member 114 is inserted into the outer cylinder 113 in a state where the tip of the injection needle 115 is inserted into the living tissue H (see FIG. 7). The living body H is moved inside the outer cylinder member 113 through the tube of the injection needle 115 by a suction operation in which the outer cylinder member 113 slides outward from the inserted position (upward in Fig. 18). It is to be collected.

In addition, the biomolecular analysis processing system 111 according to the present embodiment is connected to a biomolecular analysis device 117 which analyzes the biological tissue H collected by the syringe 112. Here, the syringe 112 has a built-in detector 116 which performs photodetection, fluorescence detection, polarization detection, etc., for example. The biological tissue H collected into the outer cylinder member 113 of the syringe 112 may be preliminarily treated by adding a chemical solution such as a cell lysate or a DNA effluent.

Next, the operation of the above configuration will be described. At the time of use of the biological tissue analysis processing system 111 of this embodiment, the distal end of the injection needle 115 of the syringe 112 is inserted into the biological tissue H. Then, the tube of the injection needle 115 is moved by a suction operation of sliding the piston member 114 into the outer cylinder member 113 in the outward direction (upward in FIG. 18) from the position where the piston member 114 is inserted into the outer cylinder member 113. The biological tissue H is collected into the outer cylinder member 113 through the inside.

Thereafter, the detector 116 in the syringe 112 performs photodetection, fluorescence detection, polarization detection, and the like, and the detection data is transmitted to the biomolecular analyzer 117. At this time, various biomolecular analysis processes are performed by the biomolecular analysis device 117 based on the detected data transmitted.

Therefore, the following effect is exhibited in the thing of the said structure. That is, in the biological tissue analysis processing system 111 of this embodiment, after aspirating the biological tissue H by the syringe 112, the collected biological tissue is picked up by the detector 116 in the syringe 112, for example. For example, photodetection, fluorescence detection, polarization detection, and the like are performed, and the biomolecular analysis device 117 performs the biomolecular analysis processing based on the detected data. Molecular analysis can be performed. Therefore, the analysis of various biological molecules of the living tissue H can be performed with high accuracy.

In addition, in this embodiment, it is applicable not only to collecting the biological tissue H from the body, but also when collecting cells, skin, etc. from the outside.

19 and 20 show an eighth embodiment of the present invention. This embodiment shows a modification of the sample processing after the sample of the biological tissue H1 collected by the endoscope treatment instrument 1 of the first embodiment (see FIGS. 1A to 5).

Fig. 19 is a flowchart showing the use of arraying pellets P having frozen samples of biological tissues H1 using the endoscope treatment instrument 1 of the first embodiment. Here, after step S8 of the flowchart shown in FIG. 5 of 1st Embodiment, it progresses to step S101. In this step S101, as shown in Fig. 20, the frozen pellets P are cut into rounded shapes and cut into thin pieces p1, p2, p3, ....

After this step S101, the process proceeds to the next step S102. In this step S102, the sample slide array 122 is produced by arranging the thin slices p1, p2, p3, ... cut | disconnected in step S101 on the board | substrate 121, such as a glass plate, in order.

Thereafter, the flow advances to the next step S103. In this step S103, treatment such as genome proteome analysis, analysis, and quantification of the sample slide array 122 is performed. Thereby, analysis with positional information is performed. In addition, the determination according to a use is performed in following step S104.

Therefore, the following effect is exhibited in the thing of the said structure. That is, in the present embodiment, the thin pellets having a thickness of about 10 nm to 50 µm under a microscope by continuously operating microfragmentation means such as diamond blades while intermittently pushing the frozen pellets P out of the syringe 112. , p2, p3,... and arrayed sample slide array 122 by arranging the cut thin slices p1, p2, p3,... in order on a substrate 121 such as a glass plate. ) Is produced. At this time, you may perform surface treatment or binder reagent application on the board | substrate 121 in order to make it easy to fix a thin slice. Until the sample slide array 122 is used, the sample slide array 122 is stored in a frozen or dried state, and then thawed or wetted the solution during use, and then treatment such as genomic proteome analysis, analysis, and quantification is performed. An analysis is performed, and the determination according to the use is performed. Techniques for applying frozen tissues other than those described above to a microarray can be referred to Japanese Patent Application Laid-Open No. 2004-500891 and Japanese Patent Laid-Open No. 2-161334.

In addition, this invention is not limited to the said embodiment, Of course, various deformation | transformation can be implemented in the range which does not deviate from the summary of this invention.

INDUSTRIAL APPLICABILITY The present invention is effective in a technical field using an endoscope treatment instrument and a biotissue analysis processing system, and in a technical field for performing a biomolecular analysis processing of a living tissue by performing a sampling method for tissue analysis processing.

Claims (15)

An elongated insert inserted into the living body through the treatment instrument channel of the endoscope for observing the living body, A tubular puncture needle disposed at the distal end of the insertion portion and inserted into a living tissue, Tissue collection means for collecting living tissue in a state where the puncture needle is inserted into living tissue; An endoscope treatment instrument comprising tissue processing means for subjecting biological tissue collected by the tissue collecting means to analytical processing. The method of claim 1, wherein the insertion portion has an elongated tubular body, the puncture needle is formed at the distal end of the tubular body, The tissue collecting means has an outer cylinder member detachably connected to a proximal end of the tubular body, and an axial piston member inserted into the cylinder of the outer cylinder member so as to be slidable in an axial direction. And a syringe for collecting biological tissue into the outer cylinder member through the puncture needle and the tubular body by a suction operation of sliding outwardly, The tissue processing means is an endoscope treatment instrument having a freezing device for freezing the biological tissue collected into the syringe. The endoscope treatment instrument according to claim 2, wherein the freezing device includes a Peltier element attached to the outer cylinder member of the syringe, and a power supply device for supplying electric power to the Peltier element. According to claim 1, wherein the insertion portion has a conductive elongated tubular body, the puncture needle is formed at the tip of the tubular body, The tissue collecting means includes a syringe having an outer cylinder member detachably connected to a proximal end of the tubular body, an axial piston member slidably inserted in the barrel of the outer cylinder member, and a tip portion of the tubular body. It has a tissue collection part arrange | positioned, It has a tissue collection mechanism which collects biological tissue inside the said tissue collection part through the puncturing needle by the suction operation which slides the said piston member to the outer direction of the said outer cylinder member, The tissue processing means is an endoscope treatment instrument having a freezing device for freezing the biological tissue collected into the tissue collection unit. The endoscope treatment instrument according to claim 2 or 4, wherein the tissue processing means has detachment means for detaching biological tissue frozen by the freezing device from the endoscope treatment instrument. The tissue processing means according to claim 1, wherein the tissue processing means has a treatment member for performing an analysis process on the biological tissue collected by the tissue collecting means, and the treatment member is brought into contact with the living tissue to An endoscope treatment instrument having a biomolecule analysis section for performing biomolecule analysis. The endoscope according to claim 2 or 4, wherein the tissue processing means has biological tissue preservation means capable of stably preserving biological molecules in the biological tissues while keeping the frozen tissues frozen in the freezing apparatus. Dragon aids. 8. The biological tissue preservation means according to claim 7, wherein the biological tissue preservation means comprises a preservation reagent capable of stably preserving the biological molecules in the frozen tissue and a deactivation of the biological molecules by inhibiting the activity of the degrading enzyme on the biological molecules. An endoscope treatment instrument having means for maintaining in a low temperature environment that can be prevented. The biological tissue according to claim 2, wherein the tissue processing means includes a sensor needle protruding from the piston member of the syringe, and the sensor needle connected to the sensor needle to puncture the sensor needle on the biological tissue collected into the tissue collection unit. An endoscope treatment instrument having a biomolecule analysis section for performing an analysis. Tissue collection means for sucking and collecting living tissue; A biological tissue analysis processing system comprising tissue processing means for performing analytical processing on the biological tissue collected by the tissue collecting means. The biological tissue analysis processing system according to claim 10, wherein the tissue processing means is a means for performing at least one of freezing, freeze drying, splitting, crushing, ethanol fixation, and formalin fixation on the living tissue. Endoscopy to observe in vivo, An endoscope treatment instrument is inserted into the living body through the treatment instrument channel of the endoscope, The endoscope treatment instrument, the elongated insertion portion is inserted into the living body through the treatment instrument channel, A tubular puncture needle disposed at the distal end of the insertion portion and inserted into a living tissue, Tissue collection means for collecting living tissue in a state where the puncture needle is inserted into living tissue; A biological tissue analysis processing system comprising tissue processing means for performing analytical processing on the biological tissue collected by the tissue collecting means. The said tissue extracting means has an outer cylinder member detachably connected to the base end part of the said tubular body, and the axial piston member inserted slidably in the axial direction in the cylinder of this outer cylinder member, The said And a syringe for collecting biological tissue into the outer cylinder member through the puncture needle and the tubular body by a suction operation of sliding the piston member outwardly of the outer cylinder member. The tissue processing means has a living tissue freezing mechanism for freezing living tissue collected into the syringe, The biological tissue freezing mechanism includes a Peltier element attached to the outer cylinder member of the syringe, and a power supply device for supplying electric power to the Peltier element. The said tissue collecting means has an outer cylinder member detachably connected to the base end part of the said tubular body, and the axial piston member inserted in the cylinder of this outer cylinder member so that a axial sliding is possible, The said And a syringe for collecting biological tissue into the outer cylinder member through the puncture needle and the tubular body by a suction operation of sliding the piston member outwardly of the outer cylinder member. The tissue processing means includes a treatment member for performing an analysis process on the biological tissue collected into the syringe, and by contacting the treatment member with the living tissue, a biomolecular analysis portion for performing biomolecular analysis of the living tissue. Having biological tissue analysis processing system. Inserting an endoscope treatment instrument having a tubular puncture needle into the body through the treatment instrument channel of the endoscope for observing the body; Inserting the puncture needle into living tissue; A tissue collection step of collecting living tissue into the endoscope treatment instrument in a state where the puncture needle is inserted into living tissue; A tissue freezing treatment step of performing freezing treatment for analysis on biological tissues collected by this tissue collecting step, And a step of taking the pellet of the frozen piece of biological tissue frozen in the tissue freezing treatment step from the endoscope treatment tool and sending it to a tissue analysis processing part which is a later step.

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