KR20080016396A - Antibacterial nano-fibers containing silver nano-particles and preparation method thereof - Google Patents

Abstract

An antibacterial nano-fiber containing silver nano-particles and a preparation method thereof are provided to mix silver nano precursor materials into the spun solution and to remove the remaining organic solvent completely. A fiber forming high polymer and silver metal salt are dissolved in the solvent so that the spinning solution is manufactured. The spinning solution is electrically spun. The electrically spun fiber is annealed at the air atmosphere so that an insoluble fiber is manufactured. Thereafter, the insoluble nano-fiber is annealed at the inner gas atmosphere as a carbonation process. As an activation process, at the steam atmosphere, the insoluble nano-fiber is also annealed. The silver metal salt is silver nitrate, sliver sulfate or silver chloride. The annealing temperature at the insoluble process is below 350°C. The annealing temperature at the carbonation process or the activation process is below 1000°C. Further, the spinning solution additionally consists of a photocatalyst.

Description

Silver nano-containing antimicrobial nanoparticles and method for producing the same {Antibacterial nano-fibers containing silver nano-particles and preparation method

1 is a manufacturing process of the antimicrobial nanofibers according to the present invention.

Figure 2 is a graph showing the electrical conductivity of the spinning solution prepared according to an embodiment of the present invention.

Figure 3 is a scanning electron micrograph of the antimicrobial nanofibers prepared according to an embodiment of the present invention. (A) 1 wt.% AgNO ₃ , (b) 5 wt.% AgNO ₃ , (c) 10 wt.% AgNO ₃ , (d) 20 wt.% AgNO ₃ .

Figure 4 is a thermogravimetric (TGA) graph of the anti-nano nanofibers prepared according to one embodiment of the present invention.

Figure 5 is a transmission electron micrograph of the antimicrobial nanofibers prepared according to the embodiment of the present invention.

(a) 1 wt.% AgNO ₃ , (b) 5 wt.% AgNO ₃ , (c) 10 wt.% AgNO ₃ , (d) 20 wt.% AgNO ₃ .

Figure 6 is a graph showing the X-ray photoelectron spectroscopy (XPS) of the antimicrobial nanofibers prepared according to one embodiment of the present invention.

(a) 1 wt.% AgNO ₃ , (b) 5 wt.% AgNO ₃ , (c) 10 wt.% AgNO ₃ , (d) 20 wt.% AgNO ₃ .

Figure 7 is an image photograph according to ultraviolet (UV) irradiation of the antimicrobial nanofibers prepared according to an embodiment of the present invention

Figure 8 is a transmission electron micrograph according to the ultraviolet (UV) irradiation of the antimicrobial nanofibers prepared according to the embodiment of the present invention. (a) 1 wt.% AgNO ₃ , (b) 5 wt.% AgNO ₃ .

9 is an antimicrobial test image using antimicrobial nanofibers prepared according to one embodiment of the present invention: a photo of a disc zone (diffusion) assay.

(a) control (PAN nanofiber), (b) AgNO ₃ / PAN (5/95 wt.%),

(c) AgNO _{3 /} PAN (5/95 wt.%) UV irradiation for 2 hours, (d) AgNO _{3 /} PAN (5/95 wt.%),

(e) AgNO _{3 /} PAN (5/95 wt.%) UV irradiation for 2 hours.

Figure 10 is a graph of obtaining the optical density (OD) using the (A) bacterial culture photograph, and (B) colorimetric meter using the antimicrobial nanofibers prepared according to an embodiment of the present invention.

11 is a scanning electron micrograph of the antimicrobial infusible nanofibers prepared according to an embodiment of the present invention; (a) 1 wt.% AgNO ₃ , (b) 5 wt.% AgNO ₃ , (c) 10 wt.% AgNO ₃ , (d) 20 wt.% AgNO ₃ .

12 is a transmission electron micrograph of the antimicrobial infusible nanofibers prepared according to an embodiment of the present invention. (a) 1 wt.% AgNO ₃ , (b) 5 wt.% AgNO ₃ , (c) 10 wt.% AgNO ₃ , (d) 20 wt.% AgNO ₃ .

Figure 13 is an antimicrobial test image of the antimicrobial infusible nanofibers prepared according to an embodiment of the present invention: a photo of the disc zone (diffusion) assay.

(a) AgNO _{3 /} PAN (1/99 wt.%), (b) AgNO _{3 /} PAN (1/99 wt.%) UV irradiation for 2 hours,

(c) AgNO _{3 /} PAN (5/95 wt.%) UV irradiation for 2 hours.

Figure 14 is the antimicrobial test results using the antimicrobial incombustible fiber prepared according to an embodiment of the present invention. (A) Culture culture evaluation photographs and (B) a graph showing the number of bacteria.

(a) cell-control, (b) membrane-control, (c) AgNO ₃ / TiO ₂ (1/1 wt.%), (d) AgNO ₃ / TiO ₂ (5/5 wt.%).

Figure 15 is an XRD graph of a sample carbonized at 800 ℃ antimicrobial nanofibers prepared according to the present invention.

(a) 0% AgNO ₃ , (b) 1% AgNO ₃ , (c) 5% AgNO ₃ , (d) 10% AgNO ₃ , (e) 20% AgNO ₃ .

Figure 16 is a transmission electron micrograph of the antimicrobial carbon nanofibers prepared according to the embodiment of the present invention.

(a) 1% AgNO ₃ , (b) 5% AgNO ₃ , (c) 10% AgNO ₃ , (d) 20% AgNO ₃ .

Figure 17 using the antimicrobial carbon nanofibers prepared according to an embodiment of the present invention

(A) a culture photograph evaluation experiment

(B) A graph showing the number of bacteria.

(a) control, (b) membrane control, (c) 1% AgNO ₃ , (d) 5% AgNO ₃ , (e) 10% AgNO ₃ , (f) 20% AgNO ₃ , (g) AgNO ₃ / TiO ₂ (1/1 wt.%).

Figure 18 is a graph showing the combined antibacterial activity using antimicrobial nanofibers, antimicrobial infusible nanofibers, antimicrobial carbon nanofibers prepared according to the present invention.

The present invention relates to a silver-containing antimicrobial nanofibers by electrospinning and a method of manufacturing the same.

In recent years, harmful microorganisms such as hospital infection problems caused by various resistant bacteria (SARS, etc.), pathogenic E. coli, food poisoning caused by O-157, Vibrio and Staphylococcus aureus, accidents caused by various microbial contamination, such as Legionella bacteria that grow in coolant, etc. It causes a lot of problems. In addition, nano silver is attracting the most attention as the basic desire to lead a healthy life by improving the standard of living and the well-being wind is released in various forms throughout the industry.

Silver is known to control over 650 species of bacteria, viruses and fungi while being harmless to the human body. Silver attaches to -SH group of protein cysteine composed of bacteria or virus, converts to sulfur compound, inhibits reproduction, or attaches to enzymes that work with oxygen, and acts as a catalyst to promote oxidation reaction. It is known as a semi-permanent antimicrobial that exerts antibacterial activity by acting in a special form on the part involved in digestion and respiration. Formula 1 schematically shows the antibacterial mechanism of silver.

As a general method for producing antimicrobial fibers, oil and inorganic antimicrobial substances are added to the spinning solution for melting and solution spinning, and methods for producing antimicrobial agents are applied to the spun fiber. However, according to the spinning method, fibers of about tens of micrometers in diameter are produced, and in such fibers, the ratio of the particles exhibiting antimicrobial properties to the surface is extremely small, thereby limiting the antimicrobial properties.

In the case of organic antimicrobial agents, antimicrobial properties are degraded due to decomposition and deformation of the antimicrobial agent during melting and solution spinning.In the case of inorganic antimicrobial agents, silver compounds are attached to porous ceramics and are manufactured in several to several tens of micrometers. Manufacturing had limitations.

In addition, when the antimicrobial agent is applied to the fiber produced by melting and solution spinning to show antimicrobial properties, the cost may increase due to secondary processing such as an application process and problems such as desorption of the coating material when applied to water quality / air purification. In addition, due to the small surface area of the antimicrobial material, it was unreasonable to show effective antimicrobial properties.

In order to overcome the disadvantages of the existing technology, a method of containing silver particles into fibers by electrospinning has recently been developed.

Electrospinning technology, which is being actively researched recently, can produce fibers with a nano-size of less than ~ 1 μm in diameter, thus providing information on the use of fibers limited to existing garments and industrial industries in narrow areas. , IT), biotechnology (BT), energy, environment, etc. are being expanded to all industries. Nanofibers produced by such electrospinning have a diameter of at least 1000 times smaller than those produced by melt spinning or solution spinning, which is 100 to 1000 times larger in specific surface area. By providing nanofibers with a surface area and having excellent adsorption characteristics and the spatial characteristics formed between nanofibers and nanofibers, they have characteristics that can be applied to various fields such as separation materials such as various sensors and filters, and regenerated cell supports.

In the case of the Republic of Korea Patent No. 10-0536459 as a prior art for the manufacturing technology of the nanoparticles containing silver nanoparticles, a method for producing a cellulose acetate nanofiber web containing silver using a solution of cellulose acetate and silver nitrate by the electrospinning method is described. . As another prior art, Korean Patent Publication No. 10-2006-0062661 discloses a method for producing silica nanofibers containing silver by electrospinning and heat-treating a silica sol solution containing tetraethyl orthosilicate (TEOS) and silver nitrate. It is described.

However, the silver nano-containing cellulose acetate nanofibers prepared by such a technique has a disadvantage in that it is difficult to use under severe conditions such as acidic, basic, and high temperature. In the case of the silver nano-containing silica nanofibers, spinning sections are extremely limited and manufactured by heat treatment. There are also serious problems with the strength and handleability of the fibers.

When silver nitrate is used as a silver nano precursor, it is necessary to reduce silver nitrate so that silver-silver particles are evenly distributed on the surface of nanofibers, and no harmful substances such as residual solvents or nitric acid in nanofibers after electrospinning are included. There is a need to not. In addition, sterilized bacteria or fungi fill the pores formed between the nanofibers and the nanofibers when applied to the filter, which may shorten the life span of the separation filter. However, the technology developed so far has such drawbacks. There are many problems in effectiveness, safety and mass production.

Therefore, the present invention has been made to solve the above problems, compared to the prior art regarding the antimicrobial nanofibers containing silver nanoparticles, antimicrobial nanofibers and silver nanoparticles are more effectively added It aims at providing the manufacturing method.

In view of the above, the present invention provides a antimicrobial nanofiber comprising the steps of preparing a spinning solution by dissolving a fibrous forming polymer and a silver (Ag) metal salt in a solvent and electrospinning the spinning solution. Provide a method.

The present invention also provides a method in which the silver nano precursor metal salt is silver nitrate, sulfuric acid, and silver chloride.

The present invention also provides a method of characterizing the carbon fiber precursor polymer as the fiber forming polymer.

The present invention also provides a method further comprising the step of stabilizing the spun nanofibers after the electrospinning step.

The present invention provides a method further comprising a carbonization and activation step after the stabilization step.

The present invention also provides a method comprising the particles having a photocatalytic effect such as titanium dioxide in the spinning solution.

The present invention further provides a method of reducing silver nanoparticles by ultraviolet (UV) irradiation after the electrospinning step.

Hereinafter, the present invention will be described in more detail.

1) Preparation of spinning solution

As the fibrous forming polymer, any polymer capable of forming fibers by electrospinning may be used. For example, polyacrylonitrile (PAN), polyvinyl alcohol (PVA), cellulose acetate, polyurethane, polycarbonate, etc. may be used, but is not limited thereto. In addition, a biodegradable polymer may also be used as the fibrous forming polymer.

Silver nitrate and silver sulfate can use silver chloride as said silver metal salt.

The solvent may be used alone or in combination of organic solvents such as dimethylformamide (DMF), dimethylacetamide (DMAc), alcohols (alcolol), or a water-soluble solvent such as water alone or organically. It can be mixed with a solvent and used. The spinning solution is prepared in consideration of the concentration, viscosity, surface tension, and electrical conductivity of the solution capable of spinning the fibrous forming polymer and the silver metal salt.

2) electrospinning

The prepared spinning solution is connected to an electrospinning nozzle using a solution supply device, and a high electric field (˜100 kV) is formed between a nozzle and a current collector using a high voltage generator to perform electrospinning. In this case, as the electrospinning apparatus to be used, a method generally used may be used, and an electro-blowing method or a centrifugal electrospinning method may be used. The spinning solution may be spun with or without adding titanium dioxide as a photocatalyst. The diameter of the nanofibers prepared by the above method is a nonwoven fabric composed mostly of less than 1 ㎛ in the form of a pressing immediately by a method such as calender or used, or by irradiating ultraviolet (UV) to reduce the silver nanoparticles on the surface You can also use

3) Incompatibility (stabilization) treatment

The electrospun nanofibers were transferred to a hot air circulation path using a conveyer belt to heat-treat them to less than 350 ° C. at an elevated temperature of 1-5 ° C. per minute under an air atmosphere to produce antimicrobial heat treatment and insoluble fiber. do. The heat-treated and incompatible fibers thus prepared can completely remove residual solvents present in the electrospinning process and change the thermoplastic polymer structure into a thermosetting polymer structure to improve heat resistance and handleability. The heat treatment temperature is appropriately selected from this point of view, and is generally set in the range of 200-400 ° C., preferably 250-350 ° C., more preferably 300-350 ° C. The antimicrobial infusible nanofibers produced by such a treatment can be used in various fields such as various antimicrobial filter materials or sanitary materials as such.

4) carbonization treatment

The incompatible antimicrobial nanofibers are carbonized in a temperature range of less than 1000 ° C. under vacuum or inert atmosphere (nitrogen or argon) to produce antimicrobial carbon nanofibers. At this time, the carbonization temperature is preferably adjusted in consideration of the melting point (melting point) and the sublimation point (boiling point) of the metal nanoparticles contained.

The carbonization treatment has the effect of protruding the silver nanoparticles present therein to the surface, and in particular, the higher the carbonization treatment temperature or the longer the progression of the silver nanoparticles, the greater the size of the silver nanoparticles on the surface by coalescence of the silver nanoparticles. The antimicrobial carbon nanofibers prepared in this way can be applied to various antimicrobial water purification filters, air purification filter media, sanitary materials, and high functional carbon nanofiber materials.

Although the temperature of the said carbonization process is less than about 1000 degreeC, it is preferable to carry out below the temperature (melting point of silver about 960 degreeC) which does not inhibit silver containing effect as it contains silver. The temperature of the carbonization treatment is also not particularly limited within the range in which the aforementioned effects can be obtained, but is approximately 800-950 ° C, more preferably 850-900 ° C, and more preferably 810-830 ° C. Do it.

5) activation treatment

In addition, the incompatible nanofibers or carbonized carbon nanofibers are activated at a temperature range of less than 1000 ° C. under a steam atmosphere to prepare an antimicrobial activated carbon nanofiber having an ultra high specific surface area of 500 to 2500 m 2 / g. The antimicrobial activated carbon nanofibers prepared in this way can be applied to various industrial fields such as ultra high performance water purification, filter media for air purification, and sanitary materials by simultaneously providing antimicrobial effect of silver with the effect of activated carbon fiber.

This activation treatment temperature is also not particularly limited, but is performed at 500-950 ° C. in consideration of the fact that silver is contained.

Example

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited only to the following examples.

Example  One : Silver nano  contain Of antimicrobial nanofibers  Produce

PAN (molecular weight 160,000 primary reagent, Sigma-Aldrich, USA) and silver nitrate (AgNO3, purity 99.999%, primary reagent, Sigma-Aldrich, USA) are mixed with N, N -dimethylformamide (DMF, primary reagent, Sigma-Aldrich, USA) was adjusted to a concentration of 10 wt.% Solvent to prepare a spinning solution. At this time, the content of silver nitrate was 1, 5, 10, 20 wt.% Relative to the PAN, and after stirring for 24 hours while adjusting the temperature of the solution to 60 ℃, cooled to room temperature and transferred to the electrospinning nozzle I was. In addition, silver nitrate and titanium dioxide (TiO ₂ ) were mixed with PAN in the same ratio, and a spinning solution was prepared using DMF as a solvent. While discharging the polymer transferred to the electrospinning nozzle at 0.5cc / g per hole using a metering pump, a high voltage generator is applied to the electrospinning nozzle and a voltage of ˜50 kV is applied, and the distance between the spinneret and the current collector is 20 It was kept at cm and electrospinning was performed.

2 shows the solution electrical conductivity of the spinning solution. As shown in the figure, as the content of silver nitrate increases, the electrical conductivity of the solution increases linearly. Commonly known electrospinning process variables include control of fiber diameter, structure and morphology by various process variables such as solution concentration, viscosity, electrical conductivity, surface tension, applied voltage, and spinning environment. It is possible to do

3 shows a scanning electron micrograph of the nanofibers obtained by electrospinning. As shown in the figure, a silver nano-containing nanofiber nonwoven fabric composed of an average diameter of 100-300 nm was obtained according to the content of silver nitrate, and the diameter of the fiber tended to decrease slightly as the content of silver nitrate increased. This may be due to the improvement of electrical conductivity. It can be seen that the diameter distribution of the prepared nanofibers is also very uniform.

Figure 4 shows a thermogravimetric graph under the nitrogen atmosphere of the nanofibers obtained by electrospinning. As the content of silver nitrate was increased, the final carbonization yield and thermal properties were confirmed to increase.

5 shows a transmission electron microscope photograph of the nanofibers obtained by electrospinning. As the content of the silver nitrate increases, the silver nanoparticles were uniformly increased and dispersed, and the size distribution of the silver nanoparticles was confirmed to be very uniformly dispersed at about 5 nm to 20 nm.

Figure 6 shows the x-ray photoelectronspectroscopy (XPS) spectrum of the binding energy (eV) for each component of the silver nanofiber according to the present invention according to the silver nitrate content. As the content of nitric acid increases, the binding energy tends to increase. This phenomenon means that the thermal properties increase as shown in FIG. 4.

Example  2 : Silver nano  contain Of antimicrobial nanofibers  UV irradiation

The nanofibers prepared by the method of Example 1 were irradiated with a wavelength of 254 nm for 2 hours using an ultraviolet (UV) irradiation apparatus to obtain UV irradiated nanofibers. 7 shows a photograph of the UV irradiated part. As shown in the figure, the UV-exposed areas turned yellow. This phenomenon is believed to reduce silver ions (Ag +) that are not completely reduced by UV irradiation. Figure 7 shows a transmission electron micrograph of the UV-irradiated nanofibers. In FIGS. 8A and 8B, silver nanoparticles were grown to about 20 nm in size by UV irradiation with nanofibers prepared by adding 1 wt.% And 5 wt.% Of silver nitrate, respectively. This result is presumed to be a phenomenon caused by the reduction of unreduced silver ions and the like at the same time by the combination of silver ions and silver ions by UV irradiation.

Example  3: Silver nano  contain Of antimicrobial nanofibers  Antimicrobial properties

The antimicrobial activity of the silver nano-containing antimicrobial nanofibers prepared by the methods of Examples 1 and 2 was evaluated as follows. A sample was prepared by cutting the silver nano-containing nanofiber nonwoven fabric prepared by the method of Examples 1 and 2 into a disk having a diameter of 1 cm, and the test bacterium was known to be a pathogenic bacterium known as a representative purulent bacterium present in skin or nasal cavity. Species Staphylococcus aureus ATCC 12600 were incubated with (BHI broth). The cell culture was evaluated by incubating the sterilized sample + 3.6ml BHI medium + 0.4ml bacteria culture in a 37 ℃ shaking incubator for 12 hours. Cells were used diluted to 1 × 10 6 CFU / mL (CFU: colony forming unit).

9 shows the results of the disc zone assay experiment. In the case of nanofibers not containing silver nitrate, as shown in FIG. 9 (a), the growth of white bacteria was observed. In FIG. 9 (b), the cut and pasted portions of the sample appeared black. It indicates that bacteria did not grow and died. Therefore, in the case of Examples 1 and 2 it was found that the antibacterial activity is large.

10 (A) and (B) show photographs and results for measuring the amount of strain using a 450-nm visible-UV spectrophotometer (UV-visible spectrophotometer) to measure the bacterial density of the bacterial culture. As shown in FIG. 10 (A), when the bacteria grew, the medium appeared yellow and opaque, but when the bacteria did not grow, the transparent result was shown. As shown in FIG. 10 (B), all of the experimental groups showed 90-99% bacterial growth inhibitory activity, and the antibacterial activity was large.

Example  4 : Silver nano  Antibacterial Incompatibility  Nano Fiber Manufacturing

The silver nano-containing antimicrobial nanofibers prepared by the method of Example 1 were oxidized to 300 ° C. while being heated to 1 ° C. per minute using a hot air circulation furnace under an air atmosphere to obtain an incompatible fiber. 11 shows a scanning electron micrograph of an incompatible silver nano-containing nanofiber. It is effective to completely remove the residual organic solvent remaining in the electrospun nanofiber by heat treatment, and as shown in FIG. 11, the fiber diameter was mostly in the range of 100 to 300 nm, and the fiber diameter decreased as the content of silver nitrate increased. Showed a tendency to

12 shows a transmission electron micrograph of the incompatible nanofibers. The size of the silver nanoparticles is dispersed in the range of about 5-20 nm, and it was confirmed that the silver nanoparticles also increase as the content of silver nitrate increases during electrospinning.

Example  5: Silver nano  Antibacterial Incompatibility  Antimicrobial Properties of Nanofibers

The antimicrobial characteristics of the silver nano-containing infusible nanofibers prepared by the methods of Examples 1 and 4 and the silver nano / TiO ₂ -containing incompatible nanofibers were evaluated by the method of Example 3. Figure 13 shows the results of the disc zone (diffusion) assay experiment. As shown in the photograph, it was confirmed that all of the incompatible fibers exhibited excellent antibacterial properties. In particular, it can be seen that the content of silver nitrate and titanium dioxide is 5/5 wt.% In FIG. 13 (c), which is relatively larger than that of (a) and (b).

14A and 14B show photographs and cell counting results of diluting the broth culture to 1/10000 and smearing the BHI agar plate. The white part in the picture shows the part where bacteria grew. As shown in the figure, all of the experimental groups showed more than 95% of bacterial growth inhibitory ability, which was confirmed to be excellent in antibacterial activity.

Example  6: Silver nano  Antibacterial Carbon Nano Fiber Of Activated Carbon Nano Fiber  Manufacture and antimicrobial properties

The silver nano-containing nanofibers prepared in Example 1 were infusified by the method of Example 4, and then carbonized to 800 ° C. in an inert atmosphere to produce silver nano-containing carbon nanofibers.

Silver nano activated carbon fiber production was heated to an inert atmosphere up to 800 ℃ and then activated for 1 hour using steam at 800 ℃ temperature. The B.E.T specific surface area of this activated sample was about 1200-1500 m 2 per gram irrespective of silver nano content.

15 shows the results of XRD (X-ray diffraction) analysis after carbonization treatment. As the content of silver nitrate increased to 1-20 wt.%, It was confirmed that the intensity of the crystal peak of the silver nanoparticles was increased.

Fig. 16 shows a transmission electron micrograph after carbonization treatment. After the carbonization treatment at 800 ° C., it was confirmed that the silver nanoparticles were uniformly dispersed in the 5-20 nm range on the surface of the carbon nanofibers.

The antimicrobial properties of the carbonized carbon nanofibers were evaluated in the same manner as in Example 3. 17 (A) and (B) show photographs and cell counting results of the broth culture diluted to 1/10000 and plated on BHI agar plat. As shown in the figure, it was confirmed that even after the carbonization treatment, the antibacterial property was very excellent up to 95-99.99%.

18 is a graph showing the results summarized by combining the antimicrobial properties of the antimicrobial nanofibers, antimicrobial infusible nanofibers, antimicrobial carbon nanofibers prepared by the present invention. It was found that the antimicrobial properties were very excellent in electrospinning, incompatibility, and carbonization treatment. From these results, the antimicrobial nanofibers, antimicrobial infusible nanofibers, and antimicrobial carbon nanofibers / antibacterial activated carbon Various antimicrobial filter materials, functional antibacterial products, and high performance antibacterial medical products using nanofibers are expected to be applicable to various fields throughout the industry.

The silver nano-containing antimicrobial nanofibers according to the present invention are manufactured by mixing and spinning a silver nano precursor material in a spinning solution, and may contain more uniform and fine silver nano particles in nanofibers, thereby exhibiting excellent antimicrobial properties. In addition, by heat treatment to completely remove the residual organic solvent remaining in the electrospun fibers, infusible and stabilized fibers, thereby providing an antimicrobial nanofibers with improved handleability. In addition, there is an effect of providing a high functional carbon nanofibers having improved antimicrobial properties by carbonizing, activating the incompatible nanofibers in an inert atmosphere.

Claims (10)

Preparing a spinning solution by dissolving a fibrous forming polymer and a silver (Ag) metal salt in a solvent; Electrospinning the spinning solution; And The method of producing a silver nano-containing antimicrobial nanofibers, characterized in that it comprises an insolubilization step of producing an incompatible fiber by heat-treating the electrospun fibers in an air atmosphere. The method of claim 1, further comprising a carbonization step of heat-treating the incompatible nanofibers under an inert atmosphere. The method of manufacturing silver nano-containing antimicrobial nanofibers according to claim 2, further comprising an activation step of heat treatment in a steam atmosphere. The method of claim 1, wherein the silver metal salt is silver nitrate, silver sulfate, or silver chloride. The method of claim 1, wherein the fibrous forming polymer is a carbon fiber precursor or has biodegradable characteristics. The method of claim 2, wherein the heat treatment temperature of the infusibilization step is 350 ° C. or less. The method of claim 3, wherein the carbonization or activation step is performed at a temperature of 1000 ° C. or less. The method of manufacturing silver nano-containing antimicrobial nanofibers according to claim 1, further comprising a photocatalyst in said spinning solution. The method of claim 8, further comprising the step of UV irradiation treatment on the electrospun nanofibers. Silver nano containing antimicrobial nano fiber manufactured by the method of any one of Claims 1-7.

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