KR20070060052A - A manufacturing method of water soluble chitosan - Google Patents

A manufacturing method of water soluble chitosan Download PDF

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KR20070060052A
KR20070060052A KR1020070043375A KR20070043375A KR20070060052A KR 20070060052 A KR20070060052 A KR 20070060052A KR 1020070043375 A KR1020070043375 A KR 1020070043375A KR 20070043375 A KR20070043375 A KR 20070043375A KR 20070060052 A KR20070060052 A KR 20070060052A
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acid
chitosan
water
molecular weight
soluble chitosan
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조석형
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조석형
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/722—Chitin, chitosan
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
    • C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
    • C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials Engineering (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

A method for preparing water-soluble chitosan is provided to keep the molecular weight of a starting chitosan intact by using a mixed solvent of organic solvent/water and adding a certain amount of an acid and reduce a total production cost owing to the simple drying step. The method for preparing water-soluble chitosan comprises a step of dispersing water-insoluble chitosan in a mixed solution of water/organic solvent, a step of adding 0.5-2moles, based on a glucose amine unit, of an acid to the solution and reacting the chitosan immersed in the resultant solution for 0.5-5hours, and a step of washing the obtained water-soluble chitosan with organic solvent and filtrating and drying the water-soluble chitosan, whereby the chitosan is neither hydrolyzed nor reduced in molecular weight.

Description

수용성 키토산 제조 방법{A manufacturing method of water soluble chitosan}A manufacturing method of water soluble chitosan

본 발명은 수용성 키토산의 제조방법에 관한 것으로 좀 더 상세하게는 불용성 물질인 키토산을 물/유기용매의 혼합용액에 분산시키고 이용액에 산을 키토산의 글루코스아민 단위에 대하여 0.5 내지 2몰의 산을 첨가하여 일정 시간 동안 침적시켜 반응시키고 키토산이 가수분해되지 않고 분자량이 감소없이 수용성 키토산의 제조방법에 관한 것이다.The present invention relates to a method for preparing water-soluble chitosan, and more particularly, to disperse chitosan, which is an insoluble substance, in a mixed solution of water / organic solvent, and add 0.5 to 2 moles of acid to the glucose-amine unit of chitosan. The present invention relates to a method for preparing water-soluble chitosan without reacting by depositing for a predetermined time and reacting the chitosan without hydrolysis.

키토산은 poly-β-1.4- glucosamine이라는 화학명을 갖는 천연 생체 고분자 물질로서 예로부터 게, 새우, 오징어 등의 갑각류의 껍질을 산 및 알칼리 처리하여 칼슘 및 단백질을 제거함으로서 얻어지는 키틴을 탈아세틸화하여 생성된다. 이러한 방법으로 생성된 키토산은 D-글루코사민(D-glucosamine)의 피라노스(pyranose) 단위체가 β-1,4 결합한 것으로서, 분자량이 수만 내지 수백만으로 이루어져 있다.Chitosan is a natural biopolymer with the chemical name poly-β-1.4-glucosamine, which is produced by deacetylating chitin obtained by removing calcium and protein from acid and alkali treatment of shells of crustaceans such as crabs, shrimps and squids. do. Chitosan produced in this way is a β-1,4 bond of pyranose (pyranose) units of D-glucosamine (D-glucosamine), the molecular weight is composed of tens of thousands to millions.

상기와 같은 구조를 갖는 키토산은 서로 이웃하는 분자와의 수소결합에 의해 견고하게 결합된 강고한 구조를 갖고 있고 물에는 녹지 않는 불용성이어서 사용상에 제약을 가져왔다. 이러한 키토산은 동물 사료의 첨가제로 많이 사용되고 항균작 용을 할 뿐만 아니라 혈중 콜레스테롤의 저하, 지방의 저하, 면역의 증강, 중금속 해독작용을 하는 것으로 알려졌기 때문이다. 또한 최근에는 동물 사료용 뿐만 아니라 인체의 생체조절, 노화억제, 면역력 강화, 질병예방 및 회복, 항암작용, 동맥경화예방과 같은 효능이 알려지면서 다이어트 식품을 비롯한 건강식품으로 사용되고 있으며, 그 외에도 무독성 천연식품보존제, 화장품, 인조피부, 창상.화상 치료제, 수술용 봉합사 등의 여러 용도로 사용되고 있다.Chitosan having the structure described above has a rigid structure that is firmly bonded by hydrogen bonding with neighboring molecules, and is insoluble in water, thereby limiting its use. This is because chitosan is widely used as an additive in animal feed and has antimicrobial action, and is known to lower blood cholesterol, lower fat, enhance immunity, and detoxify heavy metals. In addition, it is recently used as a health food, including diet foods, as well as for animal feed, known as the body's bioregulation, anti-aging, strengthening immunity, disease prevention and recovery, anti-cancer action, arteriosclerosis prevention, and other non-toxic natural foods It is used in various applications such as preservatives, cosmetics, artificial skin, wound and burn treatments, and surgical sutures.

이와 같은 여러 용도 중에서 동물 사료나 건강식품으로 사용할 경우 키토산을 분말상태로 하여 사용하게 되면, 키토산이 수불용성이므로 동물이나 사람의 생체 내에서 쉽게 흡수되지 못하게 되어 키토산이 갖는 생리활성에 대한 효과가 미비하다는 문제점이 있었다.When used as animal feed or health food among these various uses, when chitosan is used as a powder, chitosan is insoluble in water, so it is not easily absorbed in animals or humans, so the effect on the physiological activity of chitosan is insufficient. There was a problem.

따라서 일반적으로 동물사료용이나 식품용으로 사용하기 위해서는 키토산을 수용화시켜 사용하는 것이 일반적이다.Therefore, in general, in order to use for animal feed or food use, it is common to use the chitosan by accepting it.

키토산을 수용화시키는 방법은 묽은 산에 혼합하여 가수분해하거나 키티나아제와 같은 분해효소를 이용하여 수용성을 갖는 키토올리고당으로 변형시켜 액상화한 후 사료와 배합하고 스프레이 드라이(S/D) 또는 동결건조(F/D)를 하여 사용하고 있다. 이는 키토산의 분자량이 작기 때문에 식품보존제, 증점제 및 다이어트제 등의 용도로 사용할 경우 항균성이 낮거나 증점 효과가 없고 키토산의 응집 및 흡착성능은 키토산의 분자량과 비례한다고 알려진 바, 따라서 저분자의 키토산과 키토올리고당은 그 효과가 떨어지게 된다는 문제점이 있다. 또한, 고형화시키기 위한 스프레이 드라이나 동결건조장비와 같은 고가의 건조장비가 필요하기 때문에 제조원가의 상 승과 같은 문제점이 발생하였다. 또한 특허 10-0370929에 공지된 방법 역시 키토산의 용해 하여 효소(Chitosanase)분해하여 건조하여 분자량 20,000 내지 10,0000범위의 분자량을 얻는 방법을 고안하였으나 이 또한 효소에 의한 분자량의 감소가 일어나고 건조하는 단계로 이루어져 상기의 문제점을 가지고 있다. 또 특허 10-0481793에 농도 1.5N 이상의 염산 용액에 키토산을 침적하는 단계와; 상기 침적된 키토산을 휘발성 물질로 세척하는 단계와; 상기 세척된 키토산을 건조하여 제품화하는 단계로 이루어지는 것을 특징으로 하는 수용성 키토산의 제조방법을 제시하고 있으나 이는 고농도의 산을 사용할 뿐만 아니라 염산만을 사용하기 때문에 산의 사용에 제약이 있으며 특히 침적시간에 따라 분자량의 감소가 많은 등의 단점이 있다.The chitosan can be solubilized by mixing with dilute acid to hydrolyze or transform it into water-soluble chitooligosaccharides using a degrading enzyme such as chitinase to liquefy, mix with feed, spray dry (S / D) or lyophilization ( F / D). Since the molecular weight of chitosan is small, it is known to have low antibacterial or no thickening effect when used for food preservatives, thickeners and diets, and the aggregation and adsorption performance of chitosan is proportional to the molecular weight of chitosan. Oligosaccharides have the problem that the effect is reduced. In addition, since expensive drying equipment such as spray drying or freeze drying equipment is required for solidification, problems such as an increase in manufacturing cost have occurred. In addition, the method known in Patent 10-0370929 also devised a method of dissolving chitosan to decompose and dry the chitosanase to obtain a molecular weight in the range of 20,000 to 10,0000. It consists of the above problems. Further comprising depositing chitosan in a hydrochloric acid solution having a concentration of 1.5 N or more according to Patent 10-0481793; Washing the deposited chitosan with a volatile material; It proposes a method for producing a water-soluble chitosan, characterized in that it comprises the step of drying the washed chitosan to produce a product, which is limited to the use of acid because it uses only a high concentration of acid as well as hydrochloric acid, in particular depending on the deposition time There are disadvantages such as a large decrease in molecular weight.

본 발명은 상기의 문제점을 해결하기 위하여 키토산을 물/유기용매의 혼합용액에 분산시키고 이 용액에 산을 키토산의 글루코스아민 단위에 대하여 0.5 내지 2몰의 산을 첨가하여 일정 시간 동안 침적시켜 반응시키고 유기용매로 세척하여 여과 및 건조함으로써 키토산이 가수분해되지 않고 분자량이 감소없이 수용성 키토산의 제조방법에 관한 것이다.In order to solve the above problems, the chitosan is dispersed in a mixed solution of water / organic solvent and the acid is reacted by adding 0.5 to 2 moles of acid to the glucosamine unit of chitosan for a predetermined time. The present invention relates to a method for preparing water-soluble chitosan without washing the organic solvent, filtering and drying the chitosan without hydrolysis and reducing the molecular weight.

본 발명은 수용성 키토산의 제조방법에 관한 것으로 좀 더 상세하게는 불용성 물질인 키토산을 물/유기용매의 혼합용액에 분산시키고 이용액에 산을 키토산의 글루코스아민 단위에 대하여 0.5 내지 2몰의 산을 첨가하여 일정 시간 동안 침적시 켜 반응시키고 키토산이 가수분해되지 않고 분자량이 감소없이 수용성 키토산의 제조방법에 관한 것이다.The present invention relates to a method for preparing water-soluble chitosan, and more particularly, to disperse chitosan, which is an insoluble substance, in a mixed solution of water / organic solvent, and add 0.5 to 2 moles of acid to the glucose-amine unit of chitosan. It reacts by soaking for a predetermined time and the chitosan is not hydrolyzed and relates to a method for producing a water-soluble chitosan without a decrease in molecular weight.

본 발명에서 사용된 키토산은 새우껍질, 게껍질 등을 가성소다 용액 및 염산 등으로 처리하여 얻은 키틴을 탈아세틸화하여 얻은 α-키토산 또는 갑오징어 뼈 등을 가성소다 용액 및 염산으로 처리하여 제조한 β-키토산을 사용할 수 있다.The chitosan used in the present invention is prepared by treating α-chitosan or cuttlefish bone obtained by deacetylating chitin obtained by treating shrimp shell, crab shell with caustic soda solution, hydrochloric acid, etc., with caustic soda solution and hydrochloric acid. β-chitosan can be used.

상기 키토산은 탈아세틸화도가 50 내지 100％이고, 분자량이 20,000 내지 3,000,000인 것을 사용할 수 있으며 또한, 키토산은 시판품을 사용해도 좋다.The chitosan may have a deacetylation degree of 50 to 100%, a molecular weight of 20,000 to 3,000,000, and chitosan may be a commercially available product.

본 발명에서 사용할 수 있는 유기용매로는 알코올류로 메틸알코올, 에틸알코올, (이소)프로필알콜, (t-,이소)부틸알코올 등이고 케톤류로서 아세톤, 에틸메틸케톤, 디에틸케톤, 메틸프로필케톤, 에틸프로필케톤 등이며 디메틸펌아마이드(DMF), 디메틸아세트아마이드(DMAc), 테트라하이드로퓨란(THF), 디옥산, 벤젠, 톨루엔, 아세토니트릴, 크실렌, 헥산, 에틸렌글리콜 등을 사용할 수 있있으나 알콜류 및 케톤류를 사용하는 것이 바람직하나 특히 식품 및 가축용에 사용하는 경우에는 주정을 사용하는 것이 가장 바람직하다. 또한 유기용매는 단독 또는 물과 혼합하여 사용할 수 있으나 키토산의 분자량을 감안하여 용매에 산을 혼합하여 반응시킬 때 용해되지 않는 범위내에서 100 내지 20중량%를 사용하는 것이 바람직하다. Organic solvents usable in the present invention include alcohols such as methyl alcohol, ethyl alcohol, (iso) propyl alcohol, (t-, iso) butyl alcohol, and the like as acetone, ethyl methyl ketone, diethyl ketone, methyl propyl ketone, Ethyl propyl ketone and the like, dimethyl permamide (DMF), dimethyl acetamide (DMAc), tetrahydrofuran (THF), dioxane, benzene, toluene, acetonitrile, xylene, hexane, ethylene glycol, etc. can be used, but alcohols and It is preferable to use ketones, but it is most preferable to use alcohol, especially for food and livestock. In addition, the organic solvent may be used alone or mixed with water, but in consideration of the molecular weight of chitosan, it is preferable to use 100 to 20% by weight within the range that does not dissolve when the acid is mixed with the solvent and reacted.

상기에서, 사용되는 산은 무기산 또는 유기산이 사용될 수 있다. 상기 무기산으로는 염산 또는 인산 등을 사용할 수 있고, 그 중에서도 염산이 바람직하다. 또한, 유기산으로는 초산, 주석산, 살리실산, 시트르산, 하이드록시 초산, 링고산, 프로피온산, 젓산, 글리세린산, 아스코브산, 아디픽산, 사과산, 술포아민산, 솔비트산 또는 벤조산 등이 사용될 수 있고, 그 중에서도 초산, 젓산, 아스코브산, 시트르산, 사과산을 사용하는 것이 바람직하다. 또한 무기산 및 유기산 모두를 혼합하여 사용할 수 있다. 또한 산은 키토산의 글루코스아민 단위에 대하여 0.5 내지 2몰의 산을 첨가하는 것이 바람직하나 특히 키토산의 글루코스아민 단위에 대하여 0.5 내지 1.2몰의 산을 사용하는 것이 바람직하다.In the above, the acid used may be an inorganic acid or an organic acid. As said inorganic acid, hydrochloric acid, phosphoric acid, etc. can be used, and hydrochloric acid is especially preferable. In addition, acetic acid, tartaric acid, salicylic acid, citric acid, hydroxy acetic acid, ringoic acid, propionic acid, lactic acid, glycerin acid, ascorbic acid, adipic acid, malic acid, sulfoamine acid, sorbic acid or benzoic acid may be used. Especially, it is preferable to use acetic acid, lactic acid, ascorbic acid, citric acid, and malic acid. In addition, both inorganic and organic acids may be used in combination. The acid is preferably added with 0.5 to 2 moles of acid based on the chitosan's glucoseamine units, but particularly preferably from 0.5 to 1.2 moles of acid based on the chitosan's glucoseamine units.

실시예에 의거하여 본 발명을 상세히 설명하면 다음과 같다.The present invention will be described in detail with reference to Examples.

실시예 1Example 1

키토산(평균 분자량 20만, 입도 40메쉬) 100g을 주정 70중량% 수용액 400g에 분산시키고 여기에 염산(35%) 0.7몰(45.3g)을 첨가하여 교반하면서 3시간 상온에서 반응시켰다. 이를 여과한 후 다시 200g의 주정으로 세척한 후 여과하여 60℃에서 3시간 건조하여 수용성 키토산 분말을 얻었다. 이 수용성키토산의 분자량을 측정하기 위하여 평균분자량을 GPC로 분석하였다. 칼럼은 Shodex OHpakSB-801+SB-802+ SB-803(7.5mm ID×300mm L)을 사용하였고 용출액은 0.1M 아세트산완충용액을 사용하였으며 유속은 0.8㎖/min으로 조정하여 분석하였을 때 평균 분자량은 195,000으로 분자량의 변화가 거의 없었다.100 g of chitosan (average molecular weight 200,000, particle size 40 mesh) was dispersed in 400 g of a 70% by weight aqueous solution, and 0.7 mol (45.3 g) of hydrochloric acid (35%) was added thereto and reacted at room temperature for 3 hours while stirring. After filtration, the resultant was washed again with 200 g of alcohol, filtered and dried at 60 ° C. for 3 hours to obtain a water-soluble chitosan powder. In order to measure the molecular weight of this water-soluble chitosan, the average molecular weight was analyzed by GPC. Shodex OHpakSB-801 + SB-802 + SB-803 (7.5mm ID × 300mm L) was used as the eluent, 0.1M acetic acid buffer solution was used, and the flow rate was adjusted to 0.8ml / min. There was little change in molecular weight to 195,000.

실시예 2 Example 2

실시예 1에서 초산 1몰(37g)을 사용한 것을 제외하고는 실시예 1과 동일하게 실시하였다. 평균분자량은 사용한 키토산과 변화가 없었다. The same process as in Example 1 was conducted except that 1 mol (37 g) of acetic acid was used in Example 1. The average molecular weight was not changed from the chitosan used.

실시예 3Example 3

실시예 1에서 분자량 60만인 키토산을 사용하고 주정을 55%사용한 것을 제외하고 동일하게 실시하였다. 수용성 키토산의 분자량은 193,000이었다.Except for using chitosan having a molecular weight of 600,000 in Example 1 and 55% alcohol was used in the same manner. The molecular weight of water soluble chitosan was 193,000.

실시예 4Example 4

실시예 3에서 산을 시트르산을 사용하고 사용량은 키토산 글루코스아민 단위의 1몰(119g)을 사용한 것을 제외하고는 동일하게 실시하였다. 분자량의 변화는 사용한 키토산과 변화가 없었다.The acid was carried out in the same manner as in Example 3 except that citric acid was used and 1 mole (119 g) of chitosan glucoseamine units was used. The change in molecular weight did not change with the used chitosan.

본 발명의 수용성 키토산의 제조방법은 키토산을 유기용매/물의 혼합용매를 사용하고 일정 양의 산을 첨가하여 제조함으로써 가수분해 없이 처음 사용한 키토산의 분자량을 그대로 유지함으로써 처음에 사용한 분자량의 선택에 따라서 원하는 분자의 수용성 키토산을 제조할 수 있으며 또한 염산 뿐만 아니라 산의 종류와 관계없이 적은 양의 산으로 제조할 수 있으며 원하는 기능에 따라 분자량을 유지시켜 동물이나 사람의 생체 내에서 용이하게 용해되어 키토산의 약리효과를 향상시킬 수 있으며, 고가의 건조장비없이 간단한 건조과정으로 제품의 건조가 일어나므로 전체적인 제조단가를 절감할 수 있다.According to the method of preparing the water-soluble chitosan of the present invention, the chitosan is prepared by using an organic solvent / water mixed solvent and adding a predetermined amount of acid, thereby maintaining the molecular weight of the chitosan used for the first time without hydrolysis. Water-soluble chitosan can be prepared as well as hydrochloric acid as well as a small amount of acid regardless of acid type, and it can be easily dissolved in animal or human body by maintaining molecular weight according to the desired function. The effect can be improved, and the product can be dried by a simple drying process without expensive drying equipment, thereby reducing the overall manufacturing cost.

Claims (3)

수불용성 물질인 키토산을 물/유기용매의 혼합용액에 분산시키는 단계와 이용액에 산을 키토산의 글루코스아민 단위에 대하여 0.5 내지 2몰의 산을 첨가하여 0.5 내지 5시간 동안 침적시켜 반응시는 단계 및 유기용매로 수용성 키토산을 세척하고 여과하여 건조하는 단계로 이루어진 키토산이 가수분해되지 않고 분자량이 감소없이 제품화하는 것을 특징으로 하는 수용성 키토산의 제조방법Dispersing the chitosan, which is a water-insoluble substance, in a mixed solution of water / organic solvent, and dipping the acid in the solution to add 0.5 to 2 moles of acid to the glucosamine unit of chitosan for 0.5 to 5 hours to react. Method for producing a water-soluble chitosan, characterized in that the chitosan consisting of the step of washing the water-soluble chitosan with an organic solvent, filtered and dried without being hydrolyzed and reduced in molecular weight 1항에 있어서, 사용한 유기용매로는 알코올류로 메틸알코올, 에틸알코올(주정), (이소)프로필알콜, (t-,이소)부틸알코올이고 케톤류로서 아세톤, 에틸메틸케톤, 디에틸케톤, 메틸프로필케톤, 에틸프로필케톤이며 디메틸펌아마이드(DMF), 디메틸아세트아마이드(DMAc), 테트라하이드로퓨란(THF), 디옥산, 벤젠, 톨루엔, 아세토니트릴, 크실렌, 헥산, 에틸렌글리콜을 사용하고 유기용매는 단독 또는 물과 혼합하여 사용할 수 있으나 키토산의 분자량을 감안하여 용매에 산을 혼합하여 반응시킬 때 키토산이 용해되지 않는 범위 내에서 100 내지 20중량%를 사용하는 것을 특징으로 하는 수용성 키토산의 제조방법The organic solvent used is methyl alcohol, ethyl alcohol (alcohol), (iso) propyl alcohol, (t-, iso) butyl alcohol as alcohols, and acetone, ethyl methyl ketone, diethyl ketone, methyl as ketones. Propyl ketone, ethyl propyl ketone, dimethyl permamide (DMF), dimethyl acetamide (DMAc), tetrahydrofuran (THF), dioxane, benzene, toluene, acetonitrile, xylene, hexane, ethylene glycol It can be used alone or mixed with water, but considering the molecular weight of chitosan, when the acid is mixed in the solvent to react when the reaction method for producing a water-soluble chitosan, characterized in that 100 to 20% by weight is used within the range that chitosan is not dissolved 1항에 있어서, 사용되는 산은 무기산 또는 유기산이 사용할 수 있으며 무기산으로는 염산 또는 인산 등을 사용할 수 있고, 유기산으로는 초산, 주석산, 살리실산, 시트르산, 하이드록시 초산, 링고산, 프로피온산, 젓산, 글리세린산, 아스코 브산, 아디픽산, 사과산, 술포아민산, 솔비트산 또는 벤조산을 사용할 수 있으며 무기산 및 유기산을 혼합하여 사용할 수 있고 산의 사용량은 키토산의 글루코스아민 단위에 대하여 0.5 내지 2몰의 산을 사용하는 것을 특징으로 하는 수용성 키토산의 제조방법The acid used may be an inorganic acid or an organic acid, and hydrochloric acid or phosphoric acid may be used as the inorganic acid, and as the organic acid, acetic acid, tartaric acid, salicylic acid, citric acid, hydroxy acetic acid, lingo acid, propionic acid, lactic acid, glycerin Acids, ascorbic acid, adipic acid, malic acid, sulfoamine acid, sorbic acid or benzoic acid may be used, and inorganic and organic acids may be mixed and the amount of acid used is 0.5 to 2 moles of acid relative to the glucosamine unit of chitosan. Method for producing a water-soluble chitosan, characterized in that
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102276755A (en) * 2011-07-06 2011-12-14 北京化工大学 Photopolymerizable chitosan derivative as well as preparation method and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102276755A (en) * 2011-07-06 2011-12-14 北京化工大学 Photopolymerizable chitosan derivative as well as preparation method and application thereof

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