KR20060104009A - Composition for stabilizing vitamin c or its derivative containing a bergenin as active ingredient - Google Patents

Abstract

The present invention relates to a composition for stabilizing vitamin C or derivatives thereof containing vergenin as an active ingredient, and a cosmetic composition comprising vergenin and vitamin C or derivatives thereof as an active ingredient. Vergenin of the present invention contributes to the stabilization of the formulation containing it by effectively stabilizing vitamin C or derivatives thereof, and thus usefully used in the preparation of cosmetics containing vitamin C or derivatives thereof having high whitening effect, anti-wrinkle effect or antioxidant effect. Can be.

Bergenin, Vitamin C, Stabilization, Cosmetics

Description

Composition for Stabilizing Vitamin C or its Derivative Containing a bergenin as Active Ingredient}

The present invention relates to a composition and a cosmetic composition for stabilizing vitamin C or derivatives thereof containing vergenin as an active ingredient.

In addition to wrinkles and inelasticity, skin ages, causing roughness, dryness, blemishes, and freckles. In particular, as the amount of ultraviolet rays increases due to the destruction of the ozone layer due to environmental pollution, sunlight accumulates, causing photoaging, and various cosmetic compositions have been studied to solve these problems.

Currently, researches aiming at improving wrinkles of the skin are being actively conducted, and some of them show visible results. In particular, various clinical results have been reported for the removal of skin wrinkles of retinoids used for improving skin wrinkles, blemishes, and pigmentation in the dermatology area, especially retinol-containing cosmetic compositions. I am using it. In addition, it is reported that the cosmetic composition containing vitamin C essential for the collagen synthesis process has an effect of improving skin wrinkles, sun protection, pigmentation such as blemishes / freckles / black mushrooms (US Patent Nos. 4,938,969, 4,772,591, European Patent) 533,667). In addition, vitamin C is already known to have a melanin reducing effect, antioxidant effect, antiviral effect and the like.

However, cosmetic compositions containing vitamin C have the above useful effects, but are easily oxidized by air, moisture, light, heat, metal ions, oxygen, bases, and the like to form 2,3-diketo-L-gulonic acid. (2,3-diketo-L-gulonic acid), oxalic acid, L-threonic acid, L-xylonic acid, L-Lyxonic acid It is oxidized with) and discoloration and odor is the biggest problem in stability.

Vitamin C is involved in collagen synthesis and promotes collagen synthesis. The polypeptide chain of collagen fiber has an extension peptide (extension peptide, telopeptide) at both ends, and is synthesized in rough ER and then tripled. Combined into helices to form procollagen molecules, which are transported to the golgi apparatus, packed into secretory vesicles, and secreted by exocytosis. Degradation of telopeptide by extracellular enzymes results in tropocollagen molecules, which are combined in the extracellular matrix into collagen fibers. Herein, tropocollagen includes glycine, proline and hydroxyproline, which are the main amino acids constituting the polypeptide α-chain, and hydroxyproline forms a triple helix structure of collagen.

As such, hydroxyproline, which is important for collagen synthesis, accounts for about 10% of the collagen polypeptide and is present only in animals. This hydroxyproline is biosynthesized by prolyl hydroxylase by prolyl and α-ketoglutarate in the presence of oxygen. Acts as a cofactor, which must be present in order to biosynthesize hydroxyproline. That is, vitamin C promotes the activity of prolyl hydroxylase to promote the biosynthesis of hydroxyproline, thereby promoting the biosynthesis of collagen, which is a triple helix structure, to improve skin wrinkles. The activity of prolyl hydroxylase is inhibited by UV-A. Vitamin C also has a function of blocking UV rays, which also has the effect of blocking the inhibition of prolyl hydroxylase by UV-A.

Vitamin C has a different blocking action than conventional sunscreens because there is no area for absorption of L-ascorbic acid on the spectral lines of UV-A and UV-B. Vitamin C is more resistant to UV-A than UV-B and reduces erythema and edema caused by UV-B.

Vitamin C, which has various biological functions, is stored once it enters the skin, so it can be maintained for a long time even after discontinuing use.

Vitamin C also acts as a potent bio-oxidant in skin, blood and other tissues, and is a sugar-like structure (Sugar-) that is easily oxidized in the form of dehydro-L-ascorbic acid by receiving two electrons. It has a like structure, which prevents the oxidizing or denaturing of biological components (proteins, nucleic acids, membrane lipids, etc.) by active reactive oxygen, free radicals and peroxides in the body, thereby preventing skin aging. It blocks antioxidant activity.

In addition, vitamin C has a pigmentation inhibitory effect, which is achieved by inhibiting the production of melanin, which is closely related to pigmentation. Vitamin C has an effect of improving pigmentation caused by melanin by inhibiting the activity of tyrosinase, which is important for melanin formation, and reducing the oxidized melanin formed by the oxidation process to reduce melanin.

In addition, the physiological activity of vitamin C varies. It is involved in folate metabolism and amino acid metabolism in vivo and plays a very important role in the immune system to help phagocytosis of leukocytes, promote the migration of leukocytes during infection, inhibit infection and free the cell membrane of leukocytes It protects against radicals and increases the biosynthesis of interferon, a virus proliferation inhibitor, thereby increasing the body's resistance to various infectious diseases.

Most animals biosynthesize vitamin C from glucose (glucose). Humans lack the enzymes needed to convert glucose (glucose) to vitamin C, so they cannot feed on glucose biosynthesis. In addition, vitamin C is very sensitive to heat, light, moisture, oxygen, etc. and is unstable and water soluble, so the body has a lower storage capacity than fat-soluble vitamins. Therefore, it is necessary to continue to supply by food intake and other methods.

In the case of vitamin C deficiency, the deterioration of collagen biosynthesis causes wrinkles, fatigue, rough skin, resistance to infection, and capillary bleeding. Symptoms can occur and long-term deficiencies can cause serious illnesses such as scurvy. Therefore, a proper daily recommended dose should be taken and materials such as cosmetics as appropriate vitamin C containing transdermal absorbents should be used for skin protection.

When vitamin C is applied to the skin in a water-soluble state, vitamin C is easily absorbed through the skin barrier under non-ionized conditions, so the pH (pH) of the vitamin C solution must be 4.2 or higher to maintain the non-ionized state. When vitamin C is applied to the skin under these conditions, about 15% of the amount applied can be absorbed by the skin within 48 hours. Once vitamin C enters the skin, it stabilizes and accumulates in the skin without being removed by water or other rubs, and the amount of vitamin C accumulated is 20-40 times higher than when applied directly to the skin. 'S research is active.

However, vitamin C is very unstable in heat, light, oxygen, etc., has a great problem in stability and safety, such as exposure to the air or easily oxidized in an aqueous solution to lower the titer, discoloration, and odor. Despite these drawbacks, vitamin C has a high physiological activity and is being used steadily in pharmaceuticals and cosmetics. In addition, many studies have been conducted in the effort to synthesize and stabilize various vitamin C derivatives, and have been used.

For example, stabilizing vitamin C is magnesium ascorbyl phosphate, which is easier to convert to the L-ascorbic acid form available to the human body than other derivatives, but it is difficult to absorb the skin. Another derivative, Ascorbyl palmitate, is easily absorbed by the skin but is difficult to convert to L-ascorbic acid. In addition, efforts are being made to stabilize the encapsulation of vitamin C. However, the stabilization and efficacy are still weak, and studies on vitamin C, which are stable and easy to absorb into the skin, are being continued.

Throughout this specification, numerous patent documents are referenced and their citations are shown in parentheses. The disclosure of the cited patent documents is incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

Accordingly, the present inventors conducted a study to overcome the problems of the stability of vitamin C or its derivatives, and as a result, the present invention was confirmed that vergenin, which is a main component of Yeok tree extract, effectively stabilizes vitamin C or its derivatives. Completed.

Accordingly, it is an object of the present invention to provide a composition for stabilizing vitamin C or derivatives thereof.

In addition, another object of the present invention to provide a cosmetic composition comprising a vergenin and vitamin C or derivatives thereof as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to an aspect of the present invention, the present invention provides Bergenin (Bergenin, 4-methoxy-2- [tetrahydro-3,4,5-trihydroxy-6- (hydroxymethyl) pyran-2-yl] -α-resorcylic acid δ It provides a composition for stabilization of vitamin C or derivatives thereof comprising -lactone) as an active ingredient.

According to another aspect of the present invention, the present invention provides a cosmetic composition comprising (a) vitamin C or a derivative thereof and (b) bernine as a stabilizer for the vitamin C or a derivative thereof.

Bergenin included in the composition according to the present invention is represented by the following general formula (1), it is apparent to those skilled in the art that the isolated from the yeast tree, but also chemically synthesized can have the same effect.

Yedeok tree is a plant of the Euphorbiaceae. It is a deciduous arborescent called Yao-dong or red oak baek, and lives in the foothills and valleys of southern Korea. It can be as tall as 10 m, but it is usually shrub. Yekwood is commonly used as a bark for gastric ulcers, gastritis and liver function.

The main component of yeast tree is vergenin, which inhibits hepatotoxicity (Kim HS et al ., J Ethnopharmacol , 2000, Jan; 69 (1): 79-83; JP 11209282), or improves blood lipids. It is known to reduce body fat (Japanese Patent Laid-Open No. 1180869). However, it is not known that berginine may contribute to stabilizing vitamin C.

In the case where the vergenin included in the composition of the present invention is separated from the viburnum tree, it may be separated by the following steps:

(Iii) washing the wood with clean water and then drying to powder;

(Ii) the wood powder is (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol and normal-butanol, etc.), ( c) a mixed solvent of the lower alcohol with water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, (h) hexane and (i) diethyl ether Extracting using an organic solvent selected from the group consisting of as an extraction solvent; And

(Iii) isolating and purifying the vergenin from the extract of step (ii) using chromatography.

In the above step (iii), the sacred tree means various organs or parts of the sacred tree (eg, leaves, flowers, roots, stems, branches, bark and fruits, etc.), and preferably stems, branches, bark or roots. More preferably, it means a stem.

In the step (ii), the extraction step using the organic solvent as the extraction solvent may be carried out according to a conventional method known in the art, that is, under the conditions of conventional temperature and pressure.

On the other hand, the method of separating the vergenin from yeast tree includes not only the above-described separation method but also a conventional purification process. For example, various purification methods additionally performed, such as a separation method using an ultrafiltration membrane having a constant molecular weight cut-off value, a separation method by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), and the like May be included.

In addition, the vergenin of the present invention may be prepared in a powder state by performing an additional process such as vacuum distillation and freeze drying or spray drying.

In addition, the same effect can be obtained as that of bernine included in the composition of the present invention by using a chemically synthesized bernine. In a preferred embodiment of the present invention uses sigma Vergenin monohydrate (Sigma, B6776), but is not necessarily limited to those manufactured by the manufacturer.

According to a preferred embodiment of the present invention, the amount of bernine is included as 0.00001-20.0% by weight, and 0.0001-5.0% by weight relative to the total weight of the cosmetic composition. The amount of the vitamin C and derivatives thereof included with the vergenin in the cosmetic composition is included in an amount of 0.0001-20.0% by weight relative to the total weight of the cosmetic composition, preferably 0.1-10.0% by weight. If the content of bernine contained in the composition to stabilize the vitamin C is 0.00001% by weight or less, it is insufficient to stabilize the vitamin C. If it is 20.0% by weight or more, it does not have a synergistic effect on the stabilization of the vitamin C. Can have

Vitamin C or a derivative thereof used as an active ingredient in the cosmetic composition of the present invention includes any conventional vitamin C derivative developed as a cosmetic raw material. For example, the vitamin C or derivatives thereof may be selected from ascorbic acid, various salts of ascorbic acid (eg sodium ascorbate, magnesium ascorbate, sodium ascorbyl phosphate and ascorbyl magnesium phosphate) and esters of ascorbic acid (eg ascorbic acid). Bill acetate, ascorbyl propionate, ascorbyl palmitate and ascorbyl stearate).

As used herein, the term "stable" as used to refer to vitamin C or derivatives thereof refers to the action of inhibiting the oxidation of vitamin C or derivatives thereof by oxidation-causing factors such as heat, light and oxygen.

On the other hand, since the cosmetic composition of the present invention contains vitamin C or derivatives thereof as an active ingredient, the cosmetic composition of the present invention can be represented by various uses conventionally known to vitamin C or derivatives thereof.

Therefore, the cosmetic composition of the present invention comprising (a) a vitamin C or a derivative thereof and (b) a vergenin as a stabilizer for the vitamin C or a derivative thereof as an active ingredient, a cosmetic composition for improving wrinkles, a cosmetic composition for whitening It may be represented as a cosmetic composition for preventing skin aging and a cosmetic composition for blocking sun.

Ingredients included in the compositions and cosmetic compositions of the present invention include, as active ingredients, ingredients commonly used in the composition in addition to the above-mentioned vergenin, and include conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The compositions of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, when the composition of the present invention is used as a cosmetic composition, a flexible lotion (skin), astringent lotion, nourishing lotion (milk lotion), nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water It can be prepared in the form of a pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention is excellent in the stability of the formulation according to temperature change by stabilizing vitamin C or derivatives thereof by bernine, and excellent in titer preservation with temperature change and time.

Therefore, the cosmetic composition of the present invention containing vergenin and vitamin C, which effectively stabilizes vitamin C, has a physiological activity of vitamin C, that is, a whitening effect (eg, tyrosinase activity inhibition), an anti-wrinkle effect, and an antioxidant effect (eg, free radicals). Scavenging ability) can be effectively delivered to the skin. In addition, the composition for stabilizing vitamin C containing the vergenin as an active ingredient may be applied to the skin with the vitamin C formulation to contribute to the stabilization of the unstable vitamin C formulation, thereby effectively exhibiting the physiological activity of vitamin C. .

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Hereinafter, the prescription examples of the cosmetics containing the vergenin and vitamin C through the following prescription examples, but not all prescriptions are limited thereto.

Bergenin used in the formulation of the present invention used Sigma's Bergenin monohydrate (Sigma, B6776).

Prescription example 1: flexible lotion

A prescription example of a flexible lotion among cosmetics containing vitamin C and vergenin is as follows.

ingredient Content (% by weight) Vitamin c 2.0 1,3-butylene glycol 6.0 glycerin 4.0 PEG 1500 1.0 Sodium hyaluronate 1.0 Polysorbate 20 0.5 ethanol 8.0 Bergenin 0.1 Preservative, pigment Quantity Benzophenone-9 0.05 incense a very small amount Purified water Remaining amount Sum 100

Prescription Example 2: Nutritional Cosmetics

The following is an example of prescription of nutrient cosmetics in cosmetics containing vitamin C and vergenin.

ingredient Content (% by weight) Vitamin c 2.0 1,3-butylene glycol 5.0 glycerin 4.0 Cetearyl Alcohol 0.8 Glyceryl Stearate 1.0 Triethanolamine 0.13 Tocopheryl acetate 0.3 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.5 Carboxyvinyl Polymer 1.0 Bergenin 1.0 Preservative, pigment Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription 3: Nutritional Cream

A prescription example of a nourishing cream in cosmetics containing vitamin C and vergenin is as follows.

ingredient Content (% by weight) Vitamin c 3.0 Cetearyl Alcohol 1.5 Stearic acid 1.5 Glyceryl Stearate 1.5 Floating paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.5 Solbitan Sesquioleate 0.6 Squalane 3.0 1,3-butylene glycol 3.0 glycerin 5.0 Triethanolamine 0.5 Black Bean Extract 2.0 Bergenin 1.0 Tocopheryl acetate 0.5 Preservative, pigment Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription 4: massage cream

A prescription example of a massage cream among cosmetics containing vitamin C and vergenin is as follows.

ingredient Content (% by weight) Vitamin c 2.0 1,3-butylene glycol 4.0 Sodium magnesium silicate 0.3 glycerin 4.0 Triethanolamine 0.15 Beeswax 2.0 vaseline 5.0 Glyceryl Stearate 1.5 Tocopheryl acetate 0.1 Polysorbate 60 2.0 Solbitan Sesquioleate 0.8 Cetearyl Alcohol 2.0 Floating paraffin 40.0 Bergenin 1.0 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription 5: Pack

A prescription example of a pack of cosmetics containing vitamin C and vergenin is as follows:

ingredient Content (% by weight) Vitamin c 2.0 glycerin 2.0 PEG 4000 1.5 Polyvinyl alcohol 12.5 ethanol 7.0 Cured Castor Oil 0.8 Cellulose gum 0.3 Bergenin 1.0 Preservative, pigment Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription 6: Essence

The following is a prescription example of essence among cosmetics containing vitamin C and vergenin.

ingredient Content (% by weight) Vitamin c 2.0 1,3-butylene glycol 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octyldodeceth-16 0.4 ethanol 6.0 Bergenin 1.0 Incense, preservative, coloring Quantity Purified water Remaining amount Sum 100

Comparative Prescription Example 1

Comparative Formulation Examples 1, 2, and 3 of an essence formulation containing no vitamin C but only Bergen are as follows.

ingredient Comparative Prescription 1 Comparative Prescription 2 Comparative Prescription 3 Content (% by weight) Content (% by weight) Content (% by weight) Vitamin c 2.0 - - glycerin 10.0 10.0 10.0 1,3-butylene glycol 5.0 5.0 5.0 PEG 1500 2.0 2.0 2.0 Allantoin 0.1 0.1 0.1 DL-panthenol 0.3 0.3 0.3 EDTA-2Na 0.02 0.02 0.02 Hydroxyethyl cellulose 0.1 0.1 0.1 Sodium hyaluronate 8.0 8.0 8.0 Carboxyvinyl Polymer 0.2 0.2 0.2 Triethanolamine 0.18 0.18 0.18 Octyldodeceth-16 0.4 0.4 0.4 ethanol 6.0 6.0 6.0 Bergenin - 2.0 - Incense, preservative, coloring Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount Sum 100 100 100

Experimental Example 1: Stability Test with Temperature Change

In order to test the stability of the cosmetic formulation prepared in the present invention, the essence containing the vitamin C only (Comparative Formulation Example 1) and the essence containing the vitamin C and vergenin (Prescription Example 6) are each kept constant at 45 ℃ In a thermostatic container, stored in an opaque glass jar for 1 week, and stored in an opaque glass jar for 1 week in a completely shaded refrigerator kept constant at 4 ℃ and then measured the degree of discoloration

At this time, the degree of product discoloration was evaluated by classifying into the following six grades.

0: no change 1: very little change 2: little change

3: slightly worse 4: very badly 5: very severely

Discoloration evaluation stability test result by temperature change Temperature Discoloration degree of test substance Comparative Prescription Example 1 Prescription Example 6 45 ℃ 4.0 One 4 ℃ 3.0 0

As shown in Table 8, it can be seen that the cosmetic composition of Formulation Example 6, which contains Bergenin together with vitamin C, is much weaker than the cosmetic composition of Comparative Formulation Example 1, which contains only Vitamin C and does not contain Bergenin. there was. Therefore, it was found that the decrease in temperature stability of the cosmetics by vitamin C was overcome by the addition of bernine.

Experimental Example 2: Potency preservation test with temperature change and time

In order to test the stability of the cosmetic formulation when the cosmetic containing vitamin C contains berenin, an essence containing only vitamin C (Comparative Formulation Example 1) and an essence containing vitamin C and Bergenin (prescription example 6) Were stored in an opaque glass jar in a thermostat kept constant at 0 ° C., 20 ° C. and 45 ° C., respectively. The amount of L-ascorbic acid remaining after storage for 12 weeks was measured using high performance liquid chromatography (HPLC).

Titer Preservation of Formulations over Temperature and Over Time Aid group 0 week / residual rate 1 week / survival rate 2 weeks / residual rate 4 weeks / residual rate Comparative Prescription Example 1 0 ℃ 100% 93% 85% 71% 20 ℃ 100% 89.1% 77.4% 50.1% 45 ℃ 100% 82.1% 51.1% 5.1% Prescription Example 6 0 ℃ 100% 98.9% 98.7% 98.1% 20 ℃ 100% 98.7% 97.8% 97.7% 45 ℃ 100% 98.1% 97.9% 97.3%

As can be seen in Table 9, the essence of the present invention (Prescription Example 6) containing vitamin C and bernine almost no change in the content of L- ascorbic acid until about 1 week (residual rate of 98% or more) After 4 weeks, more than about 97.3% of L-ascorbic acid was detected. On the other hand, the essence (Comparative Formulation Example 1) containing only vitamin C began to have a low residual rate of L-ascorbic acid after 1 week, and showed a very low residual rate at 4 weeks.

Experimental Example 3: Whitening Effect Experiment

In order to confirm the stabilizing effect of vitamin C by the vergenin was confirmed the whitening effect of the physiological activity of vitamin C.

1.0 ml of potassium phosphate buffer solution (pH 6.8, 0.1 M, Sigma) and each sample were added to 1.0 ml of L-tyrosine (0.3 mg / ml, Sigma) at room temperature, and then maintained at 37 ° C. for 10-20 minutes. After that, 0.1 ml of tyrosinase (1250 units / ml, Sigma) was added and maintained for 10 minutes. Each reacted sample was measured for absorbance at 475 nm, and then the tyrosinase activity inhibition rate (%) was calculated according to Equation 1 below. Each sample was used after the formulation was pretreated with potassium phosphate buffer solution and filtered with a 0.5 μm filter. Untreated cosmetics were used as a control.

Sample Treatment concentration Tyrosinase Inhibition Rate (%) Average Inhibition Rate (%) Prescription Example 6 1.5 μg / ml 20.0 17.7 15.4 17.7 14 μg / ml 43.4 42.8 45.1 43.8 28 μg / ml 74.8 70.5 77.5 74.2 42 μg / ml 94.7 94.0 95.9 94.8 60 μg / ml 96.8 95.7 96.9 96.4 Comparative Prescription Example 1 14 μg / ml 21.7 24.0 23.0 22.9 28 μg / ml 61.3 66.9 60.5 62.9 42 μg / ml 93.2 94.0 95.9 93.4 60 μg / ml 95.5 95.7 95.9 95.7 70 μg / ml 95.9 95.9 95.9 95.9 Comparative Prescription Example 2 10.5 mg / ml 2.1 1.2 2.7 2.0 15.0 mg / ml 3.0 4.0 3.5 3.5 45.0 mg / ml 3.5 4.5 3.5 3.83 75.0 mg / ml 4.5 5.5 4.5 4.83 80.0 mg / ml 5.4 6.4 8.4 6.73 Comparative Prescription Example 3 10.0 mg / ml 1.2 2.3 4.0 2.5 30.0 mg / ml 3.0 5.0 4.0 4.0 60.0 mg / ml 4.0 4.0 5.4 4.5 120.0 mg / ml 5.4 5.4 3.0 4.6 240.0 mg / ml 5.4 6.4 3.0 5.6

As shown in Table 10, Comparative Formulation Examples 2 and 3 can be seen that there is almost no tyrosinase inhibitory effect, Formula Example 6 containing both vitamin C and vergenin tyrosinase compared to Comparative Example 1 containing only vitamin C Inhibition rate was higher than about 20%. Therefore, it was found that the vergenin effectively stabilized vitamin C.

Experimental Example 4: Free Radical Scavenging Activity

Free radical scavenging ability was measured for Formulation Example 6 and Comparative Formulation Examples 1, 2, and 3 as follows. Free radical scavenging activity was determined by the method using 1,1-diphenyl-2-picryl-hydrazine (DPPH) described in Nature magazine by Blois (Blois, MS, Nature (1958) vol 181, 1190). DPPH is a relatively stable free radical which, when present in the radical state, exhibits maximum absorption at 517 nm and loses its absorbance when erased. DPPH was used as a product of Sigma, dissolved in methyl alcohol at a concentration of 0.15 mM. First, the formulations of Formulations 6 and Comparative Formulations 1, 2, and 3 were pre-treated with a potassium phosphate buffer solution, filtered with a 0.5 μm filter, and then prepared in 1% concentrations, respectively, in 100 wells of 96-well plates. Μl was added. 100 μl of DPPH solution was added thereto, and the mixture was left at room temperature for 30 minutes, and then absorbance was measured at 517 nm using a micro plate reader (Biotech EL-340). In this case, 100 μl of starch solution having the same concentration was used instead of the formulation of Formulation Example 6 and Comparative Formulation Example.

Aid group Free radical scavenging activity (%) Average scavenging power (%) 1st experiment 2nd experiment 3rd experiment Prescription Example 6 94.5 95.7 94.7 95.0 Comparative Prescription Example 1 90.5 92.7 91.9 91.7 Comparative Prescription Example 2 9.4 9.8 10.1 9.77 Comparative Prescription Example 3 3.3 2.7 3.5 3.20

As shown in Table 11, Formula 6 containing vitamin C and bernine together was much higher free radical scavenging ability than Comparative Example 1 containing only vitamin C, containing nothing but Comparative Example 2 containing only berninine Comparative Example 3, which does not have little free radical scavenging ability. Therefore, it can be seen that the antioxidant power of vitamin C is further increased by stabilizing the vitamin C by the vergenin.

As described above, the present invention provides a composition for stabilizing vitamin C or derivatives thereof including vergenin as an active ingredient. In addition, the present invention provides a cosmetic composition comprising a vitamin C or a derivative thereof and bernine as a stabilizer for the vitamin C or a derivative thereof as an active ingredient.

Vergenin, an active ingredient of Yeok-duk-tree, can overcome the deterioration in stability due to the temperature change of vitamin C or its derivatives and the decrease in titer preservation with temperature and time, and thus is very effective in stabilizing vitamin C or its derivatives. Therefore, the cosmetic composition of the present invention can not only stabilize the formulation, but also effectively deliver the physiological whitening, skin wrinkle improvement and antioxidant effect of vitamin C or its derivatives to the skin.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

Stabilizing composition for vitamin C or derivatives thereof containing Bergenin as an active ingredient. (a) vitamin C or a derivative thereof; And (b) a cosmetic composition comprising a vergenin as a stabilizer for the vitamin C or derivatives thereof as an active ingredient. The cosmetic composition according to claim 2, wherein the vergenin is contained in an amount of 0.00001-20% by weight based on the total weight of the cosmetic composition, and vitamin C is contained in an amount of 0.0001-20% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 3, wherein the bernine is contained in an amount of 0.0001-5.0 wt% based on the total weight of the cosmetic composition, and vitamin C is 0.1-10 wt% based on the total weight of the cosmetic composition. The composition of claim 1, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation And a formulation selected from the group consisting of wax foundations and sprays.