KR20060026938A - Pharmacuetical compositions comprising eleutheroside e having bone growth-promoting effects - Google Patents

Abstract

The present invention is to provide a bone strengthening composition effective for the prevention and treatment of various bone diseases, including osteoporosis and food and beverages, medicines or feed containing the same, to provide a bone strengthening composition that can be routinely ingested and safe and inexpensive I would like to.

Elutheheroside E of the present invention can be identified as a component isolated from the white hairy organ, osteoblast proliferation promoting activity, bone growth promotion and bone mineralization effect, which prevents and treats growth disorders, bone mineralization adjuvant and bone mineralization It can be used as food.

The present invention provides a bone mineralization composition containing elutheroside E having osteoblast proliferation promoting activity, bone growth promoting effect and bone mineralization effect and food and beverage, medicine or feed containing the same.

Elutheroside E, osteoblasts, bone growth, bone mineralization

Description

Pharmacuetical compositions comprising Eleutheroside E having bone growth-promoting effects

1 is a graph comparing the growth rate of the tibia with respect to each feed intake group, when the normal group and eleutheroside E are administered to rats.

The present invention relates to elutheroside E having bone growth and bone strengthening action. The present invention also relates to a food or beverage, a medicine, or a feed containing the eleutheroside E. Elutheheroside E of the present invention proliferates osteoblasts and has an effect of promoting bone growth, thus preventing or treating bone diseases caused by a breakdown of bone metabolic balance, and strengthening and growing bone during growth in young people such as elementary school students. Useful for

Recently, as osteoporosis increases, the increase of various bone diseases such as an increase in fracture rate of young people is emerging as a social problem. It is estimated that there are about 10 million osteoporosis patients nationwide, and as the aging progresses, the number of patients is expected to increase to 16 million in 2010. Osteoporosis is a disease in which bone formation and bone destruction are out of balance due to the reduction of the female hormone (Estrogen hormone), mainly in postmenopausal women, but even men are seeing a decrease in bone mass with aging. There are about 2 million osteoporosis patients in this age group. In addition, the fracture rate of elementary and middle school students has more than doubled in recent 20 years. In terms of preventing osteoporosis, it is very important to increase bone mass and to obtain high maximum bone mass from a young age. As such, maintaining bone health is a global concern regardless of gender or age.

Background Art In the past, as a method of preventing or treating bone diseases, calcium diffusion by diet, light exercise, sunbathing and drug treatment have been performed. Although calcium salts such as calcium carbonate and calcium phosphate, natural calcium preparations such as right bone meal, eggshell and fish bone meal are used for calcium diffusion by diet, solubility, water absorption, In terms of taste, it is not suitable for oral ingestion. Proper exercise increases bone mass and strengthens bones, so walking and walking make bones healthy, but when your body is weak, light exercise can be cumbersome, and hardly even older people can hardly exercise. Sunbathing is good in terms of the spread of active vitamin D3, but this is not enough. Bisphosphonate or calcitonin is used for drug administration and is known to be effective in the treatment of osteoporosis. However, these substances are specialty medicines, and they require special attention, and there is a risk of side effects such as tinnitus, headache, and anorexia, so they are not usable as a continuous food ingredient.

It is an object of the present invention to provide a food material that is a bone strengthening agent that can be continuously ingested on a daily basis, which has a bone strengthening action, which is safe and inexpensive, and which can be widely used in food.

The present invention relates to a physiological activity that identifies the effect of elutheroside E on promoting bone growth and bone strengthening. In the present invention, by pharmacologically observing the bone growth effect of eluteroside (eleutheroside) E, it was intended to present the possibility of being used as a bone growth agent, bone enhancer, growth disorder treatment and growth enhancer.

Accordingly, the present inventors have completed the present invention by discovering an improvement in bone growth and bone mineralization promoting effect on elutheroside E and promoting bone growth and bone metabolism through osteoblast proliferation and calcification promoting activity.

That is, the bone-enhancing composition of the invention described in claim 1 (hereinafter referred to as "Invention 1") contains elutheroside E having osteoblast proliferation promoting activity, bone growth, and osteoporosis as an active ingredient. It is characterized by.

In addition, it is preferable that the food-drinks, medicine, or feed of this invention mix | blended and use the elutheroside E of this invention 1.

According to the present invention, it is possible to provide a safe, inexpensive and routinely ingestible bone strengthening material and food, medicine, or feed.

 Elutheheroside E used in the present invention can be observed as an ingredient that is isolated from the white hairy organs and shows an effect of promoting bone length growth significantly against the growth of normal rats in the growing season. And osteoblast proliferation promoting activity can be confirmed that the effect of strengthening bone.

 In addition, the osteoporosis composition of the present invention did not show acute toxicity in experiments by rats.

The eleutheroside E of the present invention can be prepared in various forms depending on the dosage form, and can be used as an injection, powder (or granule), drink, tablet, capsule, and the like.

The elutheroside E of the present invention can be used as a food fortifying foods as a main ingredient and an auxiliary ingredient, and can be used as food for strengthening, excipients, emulsifiers, stabilizers, sweeteners, pH adjusters, thickeners, proteins, peptides, amino acids , Lipids, polysaccharides, oligosaccharides and the like may be included in the usual food ingredients.

Hereinafter, the present invention will be described in more detail with reference to Examples.

Example 1 Measurement of Osteoblast Proliferation Activity

Osteoblast proliferation activity of eleutheroside E was measured as follows. InM1.2 cells of human osteoblasts were incubated at 37 ° C. with 5% CO ₂ -95% air in α-MEM medium containing 10% FBS, and then treated with trypsin to collect the cells. Was prepared at 1 × 10 ⁴ cell / mL. 0.5 mL of this cell preparation was dispensed in a 24-well plate and incubated under 37 ° C and 5% CO ₂ -95% air. The next day, the sample was added to each well so that it became 100, 25, and 12.5 ppm, and 2 mmol / L of alpha-glycerol phosphate which is a calcification promoter was added. Samples were dissolved in 50% DMSO and the solvent added to 1% of the culture. The cultures were exchanged daily and after 20 days the number of osteoblasts was measured using MTT. The proliferation promoting activity of osteoblasts was expressed as a relative value when the proliferation number of the control group was 100. The results are shown in Table 1.

Control Osteoblast proliferation promoting activity (control = 100) 25 ppm 130.2 ± 0.8 12.5 ppm 127.9 ± 0.5

Example 2 Bone Growth Promoting Activity

The bone growth promoting activity of elutheroside E was measured as follows. Bone growth promoting activity was assessed using Sprague-Dawley rats. Samples were dissolved in distilled water at concentrations of 0.5 and 5 mg / ml and rats were intraperitoneally administered at rates of 5 and 0.5 mg / kg body weight / day. Growth hormone was administered to the positive control group and normal saline to the normal group. Rats were given free intake of water and feed during the trial. On days 3 and 5 of the sample ingestion period (6 days), tetracycline, a fluorescent substance, was dissolved in physiological saline at a concentration of 10 mg / ml and administered at a dose of 20 mg / kg. In this embodiment, tetracycline chelates with calcium, utilizes a phenomenon in which it is deposited on bone, and observes a fluorescence line formed in the bone, thereby eleutheroside of tibia. ) The growth promoting effect by E was measured. The tibia was removed, and the length between each fluorescent line formed at the lower part of the tibial growth plate and the bone angiogenesis, ie, the grown length, was measured by administration of tetracycline. The elongation of bone in the elutheroside E-administered group and the control group was compared and the promoting effect was evaluated. As a result of the microscopic observation, it was confirmed that the eluteheroside E-administered group had an average 60.9um wide interval between two layers of the dye bands for the control group, thereby promoting bone resorption activity (FIG. 1). ).

Example 3 (Injectable)

Eluteroside E 50 mg

Sodium Metabisulfite 3.0mg

Methylparaben 0.8mg

Propylparaben 0.1mg

Proper sterile distilled water for injection

The above ingredients are mixed, prepared in 2 ml by a conventional method, and filled into 2 ml ampoules and sterilized to prepare an injection.

Example 4 (Tablet)

Eluteroside E 50 mg

Lactose 100mg

Starch 100mg

Magnesium stearate proper amount

The above components are mixed and tableted according to a conventional method for producing tablets to produce tablets.

Example 5 (Capsules)

Eluteroside E 50 mg

Lactose 50mg

Starch 50mg

Talc 2mg

Magnesium stearate appropriate amount

Capsules are prepared by mixing the above ingredients and filling into gelatin capsules according to a conventional method for preparing capsules.

Example 6 (Liquid)

Eluteroside E 50 mg

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components are mixed according to a conventional method for preparing a liquid, filled into a 100 ml brown bottle, and sterilized to prepare a liquid.

Example 7 (soft drinks)

Elutheroside E 2%

15.0% of mixed isomers,

10% fruit juice,

0.1% fragrance,

Calcium 0.1%,

72.8% of water

The above ingredients are mixed and sterilized at 90 ° C. for 15 seconds using a plate sterilizer to prepare a soft drink.

As described above, the elutheroside E of the present invention has osteoblast proliferation promoting and calcification promoting activities. In addition, it has the activity of promoting the proliferation of chondrocytes and the growth of bone. It can also be used as a supplement for capsules, tablets and the like, and is useful as a material for food and drink, medicine or feed for the purpose of preventing osteoporosis and improving symptoms. It can be added to various types of food and beverages, such as juices, juices, biscuits and the like, and frozen foods, to produce foods having a bone strengthening effect.

Claims (1)

A pharmaceutical composition for promoting bone growth, which contains elutheroside E having an osteoblast proliferation, bone growth, and bone strengthening effect as an active ingredient.

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