KR20060003917A - Whitening composition containing densifloric acid - Google Patents

Abstract

The present invention relates to a skin whitening composition containing dencifloric acid, and more particularly, dencifloric acid obtained by separating from pine needles, pharmaceutically acceptable salts thereof or derivatives thereof by confirming that densifloric acid has a function of inhibiting melanogenesis. The present invention relates to a composition for skin whitening containing floric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof as an active ingredient. In particular, the present invention is much superior to the existing vitamin C whitening effect, there is an advantage that can be applied more stably as a compound separated from natural products.

Densifloric Acid, Skin Whitening, Pine Needle

Description

Whitening composition containing densifloric acid             

1 is confirmed by ¹ H-NMR data that the material separated into pine needles dendritic acid.

Figure 2 confirms with ¹³ C-NMR data that the material separated into pine needles dencifloric acid.

Figure 3 confirms the inhibition of melanin production in dendritic acid mouse black tumor cells.

The present invention relates to a skin whitening composition containing dencifloric acid, and more particularly, dencifloric acid obtained by separating from pine needles, pharmaceutically acceptable salts thereof or derivatives thereof by confirming that densifloric acid has a function of inhibiting melanogenesis. The present invention relates to a composition for skin whitening containing floric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof as an active ingredient.

 One of the problems in women's beauty is the skin of black stools, blemishes, freckles, and dark circles. The skin whitening is more important than ever before. In general, dark skin, blemishes, freckles, and dark circles are considered to be caused by melanocytes stimulated by ultraviolet rays, abnormal hormonal balance, genetic factors, etc., and thus biosynthetic melanin pigments are deposited on the skin. . Conventionally, for such dark skin, blemishes, freckles, and dark circles, melanin inhibitors such as L-ascorbic acid or its derivatives, hydroquinone derivatives, placental extract, kojic acid and arbutin are formulated into cosmetics, and these are applied to the skin. The method of treatment improvement by application has been mainly used. However, in the case of such cosmetics, the active ingredient is erased over time due to sweating and the like, the effect cannot be maintained, the whitening effect is insufficient, and there is a problem such that the effect is exerted only on the coated part. That is, when used as a cosmetic, it is necessary to re-apply several times a day, or to apply the cosmetics to the whole body for darkening or blemishes, freckles, and dark circles of the whole body, so that the burden or inconvenience of the user was considerable. In addition, some active ingredients are irritating to the skin and have limited use or unusual odors. They may cause precipitation flocculation and lack stability, and they may be decomposed by external stimuli such as changes in the pH of the skin surface caused by light, heat or sweat. In addition, problems such as deterioration and deterioration have been pointed out. From the reasons of improving the problems with such cosmetics, whitening foods or whitening medicines for oral ingestion have also been developed. For example, there is a combination of vitamin C in this kind of food, vitamin C has a problem that can not be enough to satisfy the whitening effect is poor stability. In addition, whitening foods (Japanese Patent Laid-Open Publication No. Hei 6-16685) made by adding koji acids, cosmetics, foods and bathing agents containing the coloring rice extract (Japanese Patent Laid-Open Publication No. Hei 10-287525), and isofurabons Oral whitening agents and whitening foods (JP-A-11-269066), whitening foods containing proanthocyanidins and glutathione (JP-A-2000-60482), etc. However, they have not been able to expect a sufficient whitening effect so far, and depending on the component has the problem that there are concerns about unpredictable side effects due to oral intake. From this situation, there is an urgent need for the development of food or oral whitening agents having a sufficient whitening effect and high stability.

Thus, the inventors of the present invention, while searching for a material excellent in whitening effect from natural materials to solve the above problems, from the pine needles have separated the densifloric acid having an excellent effect to improve skin whitening stability, densiflo It has been found that lyric acid, its pharmaceutically possible salts or derivatives thereof have a very good whitening effect that can prevent or recover dark skin, blemishes, freckles, dark circles and the like, and by simply taking them orally, the whitening effect is simplified. The present invention has been completed by confirming that it can be sufficiently enjoyed.                         

Accordingly, an object of the present invention is to provide a composition for skin whitening containing densifloric acid, a pharmaceutically acceptable salt thereof or a derivative thereof.

It is another object to provide a skin lightening agent or a functional food containing the composition.

The present invention is characterized by a composition for skin whitening containing densifloric acid, a pharmaceutically acceptable salt thereof or a derivative thereof.

Also included are skin whitening agents or functional foods containing densifloric acid, pharmaceutically acceptable salts thereof or derivatives thereof.

The present invention will be described in more detail as follows.

The present invention contains densifloric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof as an active ingredient by confirming that dencifloric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof has a function of inhibiting melanogenesis. It relates to a composition for skin whitening.

First, the concentrate obtained by methanol extraction of pine needles was concentrated by adding the same amount of water and ethyl acetate, followed by layer separation. The concentrate of ethyl acetate layer was filled with reverse phase silica and eluted with a concentration gradient from 50% methanol to 100% methanol. It was confirmed that the material obtained was dencifloric acid represented by the following Chemical Formula 1.

[Formula 1]

The densifloric acid is a kind of abutane-based diter pans, and is known to exist in various plants. In the present invention, it has been found that it has a whitening effect.

On the other hand, the whitening effect is to improve or prevent dark skin, blemishes, freckles, dark circles caused by various factors such as exposure to ultraviolet rays, changes in hormonal balance, genetic programs, and make skin beautiful and transparent. Or it is intended to maintain the beautiful skin with a sense of transparency, reduce the dullness of the skin to increase the radiance, tightening and the like. In general, dark skin, blemishes, freckles, and dark circles are known to be caused by melanocytes being stimulated by ultraviolet light stimulation or hormonal balance changes, and thus biosynthetic melanin pigments are deposited on the skin. Therefore, if the production of melanin can be suppressed, it is possible to prevent and improve dark skin, blemishes, freckles and dark circles.

The melanin production inhibitory function of the densifloric acid was confirmed by a test method using cultured pigment cells. Pigment cells are melanin-producing cells which, when cultured normally, melanin accumulates and becomes black. On the other hand, if a substance having a melanin production inhibiting function is present in the culture system, the production of melanin is suppressed and the whitening becomes relatively white. From this degree of relative whitening, the melanin production suppressing function can be expected.

As a result of the test method, it was confirmed that dencifloric acid has an excellent melanin production inhibiting function when compared with vitamin C magnesium phosphate which is a known oral whitening agent. In addition, the present invention includes a pharmaceutically acceptable salt thereof, or a derivative thereof, in addition to the dendritic acid, also has a skin whitening function.

Herein, a pharmaceutically acceptable salt is a salt derived particularly from a carboxyl group of dencifloric acid (partial structure: -COOX; X represents any cationic substance) and is isolated from the natural product in the present invention. Includes what is originally included in. In the present invention, any salt that is commonly used in food or pharmaceutical compositions is not particularly limited. Examples thereof include alkali metal salts such as sodium, potassium and lithium, alkaline earth metal salts such as calcium, magnesium, barium and zinc, ammonia and methyl. Alkylamine salts, such as amine, dimethylamine, trimethylamine, ethylamine, diethylamine, triethylamine, propylamine, butylamine, tetrabutylamine, pentylamine, hexylamine, ethanolamine, diethanolamine, triethanolamine, Salts such as alkanolamine salts such as propanolamine, dipropanolamine, isopropanolamine, diisopropanolamine, other organic amine salts such as piperadine and piperidine, and basic amino acid salts such as lidine, arginine, histidine, and tryptophan Can be. Among these, alkali metal salts, alkylamine salts, alkanolamine salts and basic amino acid salts are preferable. In general, these salts are more water-soluble than the dendritic acid, which is the basis thereof, and therefore, the salts thereof are particularly preferable in the case of being applied to food of water system.

In addition, the derivative is a biochemically or artificially formed derivative, and is not particularly limited as long as it is a derivative which can be formed in the present invention. For example, a derivative having an alcohol ester group, a derivative having a fatty acid ester group, a derivative having an alkoxy group, and an alkoxymethyl group. Derivatives, glycosides, and the like.

Some of these derivatives exist in nature and can be obtained by artificially forming them. It is also possible to derivatize the derivatives of the present invention to use their salts.

Thus, by making dencifloric acid into the form of a suitable pharmaceutically acceptable salt or derivative, water solubility or usefulness can be improved. Therefore, the product which improved the handleability, quality, and the whitening effect can be set.

Therefore, the present invention is characterized by a composition for skin whitening containing densifloric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof as an active ingredient.

The skin whitening composition comprising the densifloric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof as an active ingredient may be administered orally or parenterally during clinical administration, in the form of general medicines, quasi-drugs, and functional foods. Can be used.

The composition for skin whitening is oral dosage form, for example, tablets, troches, lozenges, water-soluble or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elrics. Formulated in elixirs. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin for formulation into tablets and capsules; Excipients such as dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. Capsules contain liquid carriers, such as fatty oils, in addition to the substances mentioned above. In addition, the composition for skin whitening of the present invention can be administered parenterally, parenteral administration means a formulation such as an external preparation for the skin, injections.

Effective dosages of the compounds may vary depending on the age, physical condition, weight, etc. of the patient, but are generally administered within the range of 1-100 mg / kg body weight per day. In addition, within a daily effective dosage range is divided into once a day or several times a day.

Here, the functional food includes various foods such as confectionery, processed food, combination fat, dairy products, beverages, and the like. The shape and properties are not particularly limited, and the solid, semi-solid, gel, liquid, and powder forms Etc. may be sufficient. Since the dendritic acid or a specific derivative thereof is generally useful, it can be said to be a particularly preferred form when the food is a combination fat or oil processed product. Although there is no restriction | limiting in particular, For example, fats and oils containing a dendritic acid high combination etc. are mentioned. Moreover, the food obtained using the said combination fats and oils or processed goods as a raw material, or using it as a frying or roasting is also preferable.

In addition, an aqueous food such as a beverage, for example a soft drink, can be obtained as the food of the present invention. In particular, part or all of the densifloric acid is brought into the state of its physiologically acceptable salt or the specific derivative thereof, so that the water solubility of the densifloric acid, which is inherently useful, can be improved.

In addition, the composition for skin whitening of the present invention may be ingested as a medicine or may be blended into food as a raw material.

Here, the content as an active ingredient means that it is contained to such an extent that it exhibits its whitening effect and the like, but the content thereof is not particularly limited and may be appropriately adjusted according to the frequency of intake, the intake amount, and the purpose of use. For example, relatively low concentration is good when taken directly orally, and high concentration is preferable when used for raw materials of food.

As mentioned above, the oral whitening agent of this invention can be mix | blended with a foodstuff as a raw material, and the foodstuff which has a skin whitening effect can be obtained.

The whitening effect as a food can be made into the animal, for example, by ingesting a feed containing dencifloric acid, and assessing the improvement of pigmentation of the skin as an index.

Specifically, half of the black black mouse's back is epilated, the hair is irradiated with UV lamps for 2 weeks, and then these black mice are subjected to ultraviolet ray induction so that there is no difference in the degree of melanin pigmentation. Each group was fed with a test food containing densifloric acid in a diet low in vitamin C and fed to each group, and compared with the control group and the positive control group for the degree of pigmentation of the skin after 4 weeks. have.

From these evaluations, it was found that the dencifloric acid in the present invention can be taken orally to reliably improve pigmentation to the skin early and inconspicuous.

In the functional food of the present invention, the content of dencifloric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof is not particularly limited, and the type of densifloric acid, the type of food, the intake amount, and the intake of the citric acid contained as described above are not particularly limited. What is necessary is just to adjust suitably according to a frequency, the weight of a person to take, sex, etc., and it is although it does not restrict | limit especially, For example, it is 0.1-50 weight%, More preferably, it is 2-30 weight%. In the case of containing it as a whitening component, there is no particular restriction.

Since the functional food of the present invention contains the densifloric acid, it has various effects that they have, but in particular, has a skin whitening effect. Because it is a food, it can be easily consumed continuously and a suitable effect can be expected.

In addition, the functional food of the present invention may be blended with other physiologically active ingredients for the purpose of improving the function, in particular, synergistically improving the whitening effect, assisting in the whitening effect, and improving the absorbency. There is no particular limitation, but for example, other oral whitening ingredients that can be expected to have a synergistic effect, antioxidants that indirectly contribute to the whitening effect, oily ingredients to improve the efficiency of the body by improving the absorption efficiency, nutritional enhancement Various vitamins, minerals, amino acids and the like can be mentioned.

Other oral whitening ingredients include vitamin C and its derivatives and salts thereof, kojic acid and its derivatives, hydroquinone and its derivatives, pro-anthocyanidins, isofuravonoids and their derivatives, tannins, cysteine and glutathione, and the like. And colloidal sulfur, peptides having a specific amino acid sequence, placental extract, strawberry geranium extract, yokuining extract, golden extract, seaweed extract, wheat extract and the like. Among these oral whitening agents, particularly preferred examples thereof include vitamin C and derivatives thereof, salts thereof, pro-anthocyanidins, isofurabonoids and derivatives thereof, and tannins. Since these oral whitening agents can expect the synergistic effect with the dencifloric acid in this invention, it is preferable. When using together with the foodstuff of this invention, it is preferable to use these whitening agents and the foodstuff of this invention in the range of 0: 100-99.99: 0.01 (weight ratio). When the placenta extract and the plant extract are used in the state of extract, it is good to use this range as dry solid content. If it is this range, it will show more excellent whitening effect.

The antioxidant component is not particularly limited as long as it is commonly used in foods and the like, but for example, vitamin C and its derivatives and salts thereof, tocopherol, tocotorienol and derivatives thereof, dibutylhydroxytoluene, butylhydroxyanisol and ethylene Diamine disodium acetate, sodium ethylenediamine dibasic sodium acetate, tannins and derivatives thereof, such as molar assets and erratic acid, sulfuric compounds such as sodium sulfite, sodium hyposulfite and sulfur disulfide, ferulic acid derivatives such as γ-orizanol, and rutin And derivatives thereof, sesamin, episamine, sesaminol, sesamolin, sesamol and other lignans and their glycosides, carotenoids such as β-carotene and derivatives thereof, flavones, catechins, kelcetin, isocelcetin, leucoanthos Cyanidin, Genistin, Genistein, 6 "-O-acetylgenistin, 6" -O-malonylgenistin, Dydazine, Diyzein, 6 "-O-acetyldidazin, 6" -O-malonylidazine, glycidine, glycidine, 6 "-O-acetylglycithin, 6" -O-malonylglycithin, pue Flavonoids such as larin, quercetin, camphorol, and labyrol, quinones such as ubiquinone and vitamin K, enzymes such as superoxide dismutase, catalase, glutathione peroxidase, mallow extract, and aspergillus Tereus Extract, Licorice Oil Extract, Clove Extract, Guayak Resin, Green Coffee Bean Extract, Rice Bran Extract, Canna Extract, Salvia Extract, Buttercup Extract, Tempe Extract, Rapeseed Extract, Pimenta Extract, Blueberry Extract, Propolis Extract Oil extracts such as pepper extract, melaleuca extract, eucalyptus extract, gentian extract, buckwheat extract, red bean extract, rosemary extract, olive oil extract, soybean oil extract, soybean germ extract, thiamines and salts thereof, ribo Riboflavin such as lavine and riboflavin acetate, pyridoxine such as pyridoxine hydrochloride, pyridoxine dioctanoate, nicotinic acid such as nicotinic acid amide, and nicotinic acid benzyl, bilirubin, mannitol, tryptophan, histidine, nordihydroguaia And lactic acid. These antioxidants are known to indirectly have a whitening effect, and are also preferable because of their synergistic effect on the skin due to the anti-aging effect and the anti-aging effect on the skin due to the original antioxidant action.

The oily component is not particularly limited in addition to animal fats and oils such as soybean oil, rapeseed oil, sesame oil, olive oil, lard, bee tallow, fish oil, etc., for example, natural and chemical reactions and enzymes. MCT, MLCT, diglyceride, monoglyceride obtained by reaction, the structure maintenance which designed the structure of fatty acid, etc. are mentioned.

Various vitamins, minerals, amino acids and the like for nutritional enhancement are not particularly limited, but those specified in the food additive process sheet are preferred.

In addition, the foodstuff of this invention can mix | blend and use the raw material used for normal foodstuff.

Each said component can be suitably designed and mix | blended according to a use purpose. According to the kind of water absorbency or effect, the whitening effect can be enhanced or supplemented, or a preferred embodiment can be used. For example, isofurabons and derivatives thereof are excellent in water solubility and act on the body at the same time as the dencifloric acid in the present invention, which is generally an oil-soluble substance, and includes a tyrosinase inhibitory effect, It is anticipated that the effect of the synergy will be exerted through several metabolic pathways, and the effect will be synergistic. Moreover, the whitening food etc. which mix | blended the dencifloric acid, isoflavonoid, etc. in this invention can expect that the physiological activity, such as antioxidant and estrogen, of isoflavonoid is simultaneously activated synergistically.

Although the specific example is listed below about the foodstuff of this invention, this invention is not limited to these. Although the foodstuff of this invention does not have a restriction | limiting in particular about the form, etc., For example, in addition to a normal form, it can take the form of liquid food, enteral nutrition, health food, infant food, etc. In particular, in terms of continuous intake, rice and various seasonings, processed fats and oils such as margarine, shortening, mayonnaise, dressing may be preferred. The shape and shape are not particularly limited, and any shape may be used, such as a solid shape, a semisolid shape, a gel shape, a liquid shape, or a powder shape.

Since dencifloric acid in the food and drink of the present invention is generally an oil-soluble substance, fats and oils, processed foods, and the like are preferred as the food and drink of the present invention in terms of solubility. There is no restriction | limiting in particular as such fats and oils, For example, the combination fats and oils which melted and contained the natural or artificially obtained densifloric acid in normal fats and oils are mentioned. In addition, the dendritic acid-containing combination fat and oil may be mixed with other fats and oils, and in this case, a synergistic effect with the physiological activity effect of the trace component included in the other fats and oils can be expected.

By ingesting the food of the present invention, the effect is obtained by absorbing the densifloric acid and their physiologically acceptable salts or derivatives thereof having the skin whitening effect in the body. Since it is a form of food and drink, it does not require effort, such as cosmetics, and since it can be consumed continuously, it is preferable.

In other words, by ingesting the skin whitening composition of the present invention to express the whitening effect, it can greatly contribute to maintaining a beautiful skin with transparency by improving or preventing dark skin, blemishes, freckles, dark circles.

Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited thereto.

Preparation Example 1 Separation of Dencifloric Acid

Methanol was extracted by concentrating 10 kg of fresh pine needles from the red pine extract, and 30 g of the ethyl acetate layer, which was obtained by layer separation in an aliquot, was added to methanol extract concentrate. Elution was carried out from 50% methanol to 100% methanol in gradient. The active fractions were concentrated and purified by reversed phase high performance liquid chromatography and confirmed by nuclear magnetic resonance or mass spectrometry. The results are shown in FIGS. 1 and 2.

The compound thus separated is represented by the following Chemical Formula 1, which was identified as dencifloric acid.

[Formula 1]

Example 1: Check melanin production inhibitory function

2 ml / well of the medium was taken to a plate with six holes, and a predetermined amount of B-16 melanomer cells were seeded, followed by standing culture at 37 ° C and 5% carbon dioxide concentration. The following day, the sample sample (dencifloric acid) preparation liquid was added and mixed so that it may become predetermined concentration, and culture | cultivation was continued. On day 5 of culture, the medium was changed and sample preparation was added again. The next day, the medium was removed and the cells were collected, washed with PBS (phosphate buffered saline), and photographed. It was found that melanin was whitened at 2 ppm compared to the control group not treated with dencifloric acid.

The melanin content of the cells of the sample was dissolved in 0.85 normal potassium hydroxide and absorbance was measured at 405 nm. In addition, the evaluation of the melanin production inhibitory function was 450 ppm of 50% of melanin production of vitamin C magnesium phosphate, which is known to be effective instead of the sample preparation, but was untreated at 2.2 ppm of dencifloric acid. It was found that 50% inhibition of melanin production in the group showed that dencifloric acid had melanin suppression function of about 200 times that of vitamin C magnesium phosphate.

As mentioned above, it has been found that dencifloric acid has a phenomenal whitening effect of several hundred to two hundred times that of vitamin C magnesium phosphate widely used as a conventional oral whitening agent. Accordingly, it has been clarified that food and oral whitening agents with very good whitening effects that have never been provided can be provided.

Example 2 Confirmation of Whitening Effect in Animals

Black mice (5 weeks old, males) were preliminarily bred for 1 week with commercial feed (manufactured by CJ Co., Ltd.), and then half of the back was removed, and UV lamp 1.2 mW was applied for 5 minutes for 2 minutes on the first day. And 3 days, 1.0 mW was irradiated for 5 minutes. Subsequently, after breeding for two weeks with a low vitamin C feed (200.0 mg / kg), the black mice were divided into three groups so as not to cause a difference in the degree of melanin pigmentation induced by UV induction (7 groups each), As a low vitamin C feed, test foods containing densifloric acid were freely ingested in each group, as shown in Table 2 below. However, the control group was fed with low vitamin C feed and the positive control group with vitamin C added. The pigmentation degree of the skin 4 weeks after the start of the test was judged based on the following criteria. The results are shown in Table 3 below.

group additive Addition amount (mg / feed kg) 1 (control) - - 2 (positive control) L-Arcorbic Acid 2500.0 3 Densifloric Acid 500.0

(Evaluation Criteria and Content)

Significantly improved: Less pigmentation compared to positive control

Moderate improvement: pigmentation at the same level as the positive control

Slight improvement: moderate pigmentation between control and positive controls

No change: equal pigmentation as control

Whitening Evaluation in Black Mouse group Markedly improved Medium improvement Slightly improved No change 1 (control) 0 0 0 7 2 (positive control) 0 One 4 2 3 4 2 One 0

As shown in Table 3 above, it has been clarified that dencifloric acid can be orally ingested to improve pigmentation to the skin early, making it inconspicuous.

Example 3: Acute Toxicity of Oral Administration in Rats

Acute toxicity test was performed using 6-week-old SPF SD rats as follows.

Dencifloric acid was dissolved in distilled water for injection in two animals per group and administered orally at a dose of 1 g / kg / ml. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed. Necropsy was performed to observe abdominal and thoracic organ abnormalities. As a result, no significant clinical symptoms or dead animals were noted in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings. Therefore, dencifloric acid according to the present invention did not show a toxic change to 500 mg / kg in all rats, and the minimum lethal dose (LD ₅₀ ) for oral administration was determined to be a safe substance of 500 mg / kg or more.

Example 4: Preparation of Tablets

A tablet containing 15 mg of active ingredient was prepared by the following method.

250 g of dencifloric acid were mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. It was dried and the mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into a tablet.

Example 5: Edible Combination Oil

5.0 g of active ingredients

Soybean oil 1000.0 g

Purified densiprolic acid 1 was added to the soybean oil in the above mixing ratio, and mixed and dissolved until the whole became clear using a stirrer while maintaining a temperature of 60 ° C to prepare an edible combination fat or oil.

Example 6: Dressing

46.6 g of water

0.1 g of xanthan gum

Fructose, glucose, liquid sugar 5.0 g

5.0 g of salt

MSG 0.3 g

10.0 g of rice vinegar (acidity 10%)

Pepper quantity

1.0 g of active ingredients

32.0 g soy salad oil

At the blending ratio, the raw materials, except for soybean salad oil, are first introduced into a warmable container equipped with a stirrer, and heated until the temperature of the mixture reaches 90 ° C. while stirring at 100 rpm using a propeller stirrer and the temperature of the mixture. It was stirred for 25 minutes while maintaining at 90 ° C. Thereafter, the mixture was cooled to 20 ° C and combined with soybean salad oil to prepare a dressing. The finished dressing was a savory dressing.

Example 7: Beverage Preparation

After dissolving 500 mg of dencifloric acid according to the present invention in an appropriate amount of water, vitamin C as an auxiliary component, citric acid, sodium citrate, and oligosaccharides are added as an auxiliary component, and an appropriate amount of sodium benzoate is added as a preservative, followed by adding water to the whole amount. To 100 ml to prepare a beverage composition. At this time, taurine, myo-inositol, folic acid, pantothenic acid, etc. may be added alone or together.

As described above, the present invention does not require the burden of time or effort, and simply and continuously ingests dencifloric acid, a pharmaceutically acceptable salt thereof, or a derivative thereof as a food or oral preparation. As a result, it is possible to appropriately enjoy the whitening effect on the skin. In addition, since the dencifloric acid in the present invention can be obtained in nature, the use thereof can be assured.

Claims (5)

Dencifloric acid represented by the following formula (1), a pharmaceutically acceptable salt thereof or derivatives thereof as an active ingredient comprising a composition for skin whitening. [Formula 1]

The composition for skin whitening according to claim 1, wherein the densifloric acid is obtained from pine needles or chemically synthesized. Dencifloric acid represented by the following formula (1), its pharmaceutically acceptable salts or derivatives thereof as a active ingredient comprising a skin lightening agent.  [Formula 1]

The skin lightening agent according to claim 3, wherein the skin lightening agent is an oral dosage form. A functional food characterized in that it contains a densifloric acid represented by the following formula (1), a pharmaceutically acceptable salt thereof, or a derivative thereof as an active ingredient. [Formula 1]

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