KR20050028508A - Composition comprising an extract of phyllostachys genus or sasa borealis for prevention and treatment of hypertension disease - Google Patents

Abstract

A composition for prevention and treatment of hypertension disease comprising an extract of Bambusoideae is provided, which composition inhibits activity of ACE(angiotensin converting enzyme) that induces hypertension disease without side effects. The composition for prevention and treatment of hypertension disease comprises a crude extract of Bambusoideae as an effective component, and pharmaceutically acceptable carries and diluents, wherein the crude extract of Bambusoideae is obtained by extracting Bambusoideae with water, lower alcohol or a mixture thereof; the crude extract of Bambusoideae is obtained by extracting Bambusoideae with nonpolar solvent selected from hexane, dichloromethane and ethylacetate; the Bambusoideae is selected from Phyllostachys bambusoides, Phyllostachys nigra var. henonis, Phyllostachys pubescens and Sasa borealis; and the Bambusoideae extract is obtained from the stem or leaf of Bambusoideae.

Description

Composition comprising an extract of Phyllostachys genus or Sasa borealis for prevention and treatment of hypertension disease

The present invention relates to a composition for the prevention and treatment of hypertension containing bamboo extract and including essential hypertension.

Hypertension refers to high blood pressure continuously beyond the normal range, and clinically measured at the time of resting, the highest blood pressure (constrictor blood pressure) is 150-160mmHg or more in adults, and the lowest blood pressure (diastolic blood pressure) is 90-95mmHg or more. Treats as hypertension. In high blood pressure, only the highest blood pressure is high, and the highest blood pressure and the lowest blood pressure are both high. In general, hypertension is often referred to as the latter and the former, ie only the highest blood pressure, is when the amount of blood sent from the heart is increased and when the elasticity of the aorta is reduced, that is, some type of heart valve disease, hyperthyroidism, or aorta. It is the case of hardening and aortic aneurysm. Usually, the latter high and low blood pressures are all high, and the cause of hypertension is known (secondary or secondary) and the cause is unknown and has genetic factors (primary or Essential nature). In terms of numbers, essential hypertension is overwhelming, accounting for 90-95% of hypertension. However, as the study of hypertension progressed, it was recently found that a large number of secondary hypertensions that were identified as the cause diseases were included.

Inherent hypertension is the only subgeneity that is relatively certain, about 60% for children if the parents are hypertensive, about 20% if one of the parents is hypertension, and 5 if the parents are not all hypertension. Hypertension develops in about a percentage. The causes of secondary hypertension include renal disease (acute nephritis, chronic nephritis, pyelonephritis, hydronephrosis, renal artery stenosis, etc.), changes due to large vessels (aortic stenosis, peripheral vascular occlusion), endocrine diseases (Cushing's syndrome, pheochromocytoma, essential hyperaldosteroneism, etc.), and other gestational addictions, including those found in extreme mental anxiety and tension.

Many antihypertensive agents have been developed and used for the treatment of hypertension, which is one of the adult diseases, and currently used antihypertensive agents are diuretics and sympathetic nervous system drugs (α, 2-adrenergic antagonists, β-adrenergic agents, depending on the mechanism of action and point of action). Antagonists), vasodilators, calcium channel blockers, and angiotensin converting enzyme inhibitors (ACE Inhibitors).

Angiotensin Covering Enzyme (ACE) is a type of peptidyl dipeptidase that catalyzes the production of Angiotensin II, known as the most potent booster, and at the same time the most potent vasodilator Catalyzes the decomposition of bradykinin. Angiotensin II, a pharmacologically active substance produced by cleaving C-terminal histidine-leucine (His-Leu) of angiotensin I inactive by ACE, raises blood pressure by potent vasoconstriction and releases aldosterone from the adrenal cortex. By stimulating, it acts to preserve sodium, increasing intravascular volume. It is also known that the six amino acids at the C-terminus of angiotensin II have most of the information necessary for pharmacological action. Since ACE inhibitors that inhibit the activity of angiotensin converting enzyme (ACE) show a consistent blood pressure lowering effect, they can be used over a wide range for the treatment of hypertension, including patients with essential hypertension. In addition, ACE inhibitors not only show a significant blood pressure lowering effect, but also have good tolerability, and unlike other antihypertensive agents, have less side effects from long-term use, and thus can be used in hypertensive patients with heart disease, diabetes, asthma, etc. This is very high.

Therefore, much research is currently being conducted on ACE inhibitors. The first introduced ACE inhibitors were Teprotide, but they had a short duration of action and had to be injected into the blood vessels. Therefore, oral useful agents such as Captopril and Enalapril were used. ACE inhibitors have been developed and are currently being used as new and potent antihypertensives (Peter Sleight, The American Journal of Cardiology , 89 (2), pp 11-16, 2002: Roig, E. et al., European Heart Journal , 21 (1) , pp 53-57, 2000).

On the other hand, many studies have been carried out on the production of plant-derived ACE inhibitory peptides as well as the preparation of derivatives through chemical synthesis. Plant-derived ACE inhibitory peptides include trypsin hydrolysates of casein, peptides from porcine serum, peptides from digestive digestion of tuna, vinegar peptides, residue peptides from sake and by-products, and also lactose. Peptides produced by the hydrolysis of milk proteins by the isolation of extracellular proteases from Bacillus helveticus CP 790 have been studied. ACE inhibitory peptides using Lactobacillus Helveticus CP 790 include a, s1-casein derived peptides: alanine (Ala)-tyrosine (Tyr)-phenylalanine (Phe)-tyrosine (Tyr)-proline (Pro)-glutamic acid (Glu) Polypeptide having an amino acid sequence of (A), Arginine (Arg)-Aspartic Acid (Asp)-Methionine (Met)-Proline (Pro)-Isoleucine (Ile)-Glutamine (Gln)-Alanine Ala)-polypeptide having an amino acid sequence of phenylalanine (Phe) has been found (Walmor C., De Mello., Regulatory Peptides , 115 (1), pp31-36, 2003: Ewa, DM et al., Peptides , 24 (6), pp 791-798, 2003).

About 280 kinds of bamboo are known around the world as flowers and plants. About 70 kinds of bamboo are grown or grown in Korea. There are 11 varieties of varieties such as batting, king (bamboo), jongjong (bamboo), ojuk, dough, island, haejangjuk (Shinwoodae), gaddae, walnut, mountain porridge, and daedae. Sesame), cotton swabs, and bamboo shoots. According to Dongbobogam, Herbal Botanical Tree, and Sinnonbonchosa, bamboo has an excellent effect in treating stroke and hypertension. Especially, bamboo leaves have been used for pneumonia, bronchitis, etc. for antipyretic, expectorant, and refreshing purposes. It is used for the treatment of hypertension, atherosclerosis, and cardiovascular diseases and is introduced as a good crop for anti-cancer and anti-aging. This function of bamboo is considered to be closely related to the antioxidant effect. In addition, phytochemicals such as organic acids, dietary fiber, tannin, and benzofuran present in bamboo extracts are expected to contribute to the prevention of diseases of the circulatory system through mechanisms such as antioxidant activity, thrombolytic activity, and lipid lowering action.

Korean Patent Laid-Open No. 2001-0069130 discloses a method for preparing bamboo (Idae) leaf extract and its use as a food preservative using antibacterial activity thereof, and WO9857545 discloses a composition for lowering cholesterol containing phytosterol extracted from bamboo shoots. , US patent registration US3418311 discloses a polysaccharide having anticancer activity of bamboo.

However, none of the literature teaches or discloses that bamboo extracts are used to treat hypertension.

In order to observe the antihypertensive effect of the bamboo extract, the present inventors inhibited the ACE activity of 70% ethanol extracts and extracts of the solvents of the four kinds of bamboos (Kingdae, cotton pad, bamboo shoot, and saunder) in domestic in vitro ( in vitro ) As a result of measuring the effect, this invention was completed by confirming the outstanding effect.

An object of the present invention is to provide a pharmaceutical composition for the prevention and treatment of hypertension, including essential hypertension containing bamboo extract having an ACE activity inhibitory effect as an active ingredient.

In order to accomplish the above object, the present invention provides a pharmaceutical composition effective in the prevention and treatment of hypertension, including essential hypertension containing the crude crude extract, non-polar solvent-soluble extract as an active ingredient.

The bamboo of the present invention includes a phyllostachys bambusoides S. et Z., phyllostachys pubescens , cotton ( Phyllostachys nigra var. Henonis ) and sasa ( sasa borealis ), and can use the stem or leaf portion of bamboo have.

The crude extract includes water, ethanol, methanol, such as C ₁ to C ₄ lower alcohol or a mixed solvent thereof, preferably an extract available in 70% ethanol.

In addition, the non-polar solvent soluble extract includes an extract soluble in hexane, chloroform, dichloromethane or ethyl acetate.

Hereinafter, the present invention will be described in detail.

The bamboo crude extract of the present invention, after drying, has a volume of about 1 to 15 times the volume of bamboo stems or leaves made of small pieces, preferably about 5 to 10 times the amount of C ₁ to C ₄ such as water, ethanol, methanol and the like. of Lower alcohol or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably 1: 0.2 to 1: 3, at a extraction temperature of 20 to 100 ° C, preferably 50 to 100 ° C, for about 0.5 hour to 2 hours. The cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction and the like for one day, preferably 1 hour to 1 day is an extraction method, preferably 1 to 12 times, preferably 3 to 4 times by reflux cooling extraction method After repeated extraction, the filtered and filtered extract was concentrated under reduced pressure at 20 to 100 ℃, preferably 40 to 70 ℃ with a vacuum rotary concentrator, and the extracted residue was dried with a vacuum freeze dryer to mix water, lower alcohol or a mixture thereof. A crude crude extract soluble in a solvent can be obtained.

In addition, the non-polar solvent soluble extract of the present invention is about 1 to 10 times, preferably about 1 to 10 times of these suspensions after suspending the water-soluble extract, the lower alcohol soluble extract, and the soluble extract by the mixed solvent thereof in water. Non-polar solvents such as 5 times the volume of hexane, ethyl acetate, chloroform, dichloromethane and the like can be added to obtain a fraction 1 to 5 times, preferably 2 to 4 times. It is also possible to further carry out conventional fractionation processes (Harborne JB; Phytochemical methods: A guide to modern techniques of plant analysis. 3rd Ed ., Pp 6-7, 1998).

In a preferred embodiment of the present invention, for example, after suspending the crude crude extract in water, it is possible to obtain the bamboo extract of the present invention using a solvent in the order of hexane, dichloromethane, ethyl acetate, butanol, More preferably, hexane-soluble fractions and water-soluble fractions can be obtained by using a mixed solvent of hexane: ethanol extract: water (10: 1: 9) in the crude crude extract, and the water-soluble fraction is extracted again with dichloromethane. To obtain a water soluble fraction and a dichloromethane soluble fraction, ethyl acetate may be added to the water soluble fraction to obtain an ethyl acetate soluble fraction and a water soluble fraction, and finally the water soluble fraction is extracted with butanol. Soluble fractions and water soluble fractions can be obtained.

The crude crude extract and the non-polar solvent soluble extracts obtained above showed the inhibitory effect of ACE activity in vitro , and the inhibitory effect of ACE activity was in the order of Wangdae, Byeongjongjuk, cotton swab, and soda. In the case of the extract, it was confirmed that the ACE activity inhibitory effect of the butanol layer was the highest.

The present invention provides a pharmaceutical composition effective for the prevention and treatment of hypertension, including essential hypertension containing the crude crude extract of the present invention obtained as the active ingredient as an active ingredient.

In addition, the present invention provides a pharmaceutical composition effective for the prevention and treatment of hypertension, including essential hypertension containing the bamboo non-polar solvent soluble extract of the present invention obtained as the active ingredient as an active ingredient.

Bamboo of the present invention has been used as a herbal medicine and food for a long time there is little toxicity and side effects, so can be used with confidence even for long-term use for treatment and prevention purposes.

Pharmaceutical compositions comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical dosage forms of the extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, or may be used alone or in combination with other pharmaceutically active compounds, as well as in any suitable collection.

Pharmaceutical compositions comprising extracts according to the invention, in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. Can be formulated and used. Carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use may include various excipients, such as wetting agents, sweeteners, fragrances, preservatives, etc., in addition to water and liquid paraffin, which are commonly used to include suspensions, solutions, emulsions, and syrups. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. Administration may be administered once a day or may be divided several times.

The extract of the present invention can be administered to mammals such as mice, mice, livestock, humans, etc. by various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The present invention provides a health functional food comprising the extract and a food acceptable food supplement additive exhibiting a prophylactic effect of hypertension including essential hypertension. Examples of the food to which the bamboo extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

It may also be added to food or beverages for the purpose of preventing the hypertension, including essential hypertension. At this time, the amount of the extract in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5 g, preferably 0.3 to 1g based on 100 ml. have.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the extract as an essential ingredient in the indicated proportions, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrate is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention is described in more detail based on the following examples and experimental examples, but the present invention is not limited thereto.

Example 1 Preparation of Bamboo Crude Extracts

Timber Bamboo (Phyllostachys bambusoides S. et Z.) in Southern Forestry Experiment Station Gajwa siheomrim (Jinju, Gyeongnam) on bamboo October 2001, with the invention, maengjongjuk (Phyllostachys pubescens), somdae (Phyllostachys nigra var. Henonis), Sasa (Sasa borealis ) was used for sale, and the killing was based on three years. The collected bamboo stalks and leaves were washed with clean water and then dried for 10 days and used as samples for extraction.

After drying, 100 g of each stem or leaf made of small pieces of bamboo, 1 l of 70% ethanol was extracted three times at a predetermined time interval (12 h, 6 h, 3 h) at 50 ° C., and filtered under reduced pressure with Wattman (USA). Then, the filtrate extract was removed with 70% ethanol at 60 ° C. with a vacuum concentrator (BUCHI Laboratoriums Technik AG, Rotavapor RE-111, Germany), and the extracted residue was dried with a vacuum freeze dryer to obtain 4.4 g of bamboo dry extract. , -70 ℃ was used in the experiment while storing in the freezer.

Example 2. Preparation of Bamboo Hexane Soluble Extract

To 4.4 g of the 70% ethanol extract dried powder obtained in Example 1 was added hexane: ethanol: water = 1: 9: 1 mixed solvent 0.2 l, and then mixed three to four times to obtain 0.1 L of water-soluble fraction and hexane. After 1.5 L of soluble fraction was obtained, the hexane-soluble fraction was dried to give 0.14 g of hexane-soluble fraction.

Example 3 Preparation of Bamboo Dichloromethane Soluble Extract

       After diluting 0.2 l of dichloromethane into 0.1 L of the water-soluble fraction obtained in Example 2, the mixture was mixed three to four times to obtain 0.2 L of water-soluble fraction and 2 L of dichloromethane-soluble fraction. The dichloromethane-soluble fraction was dried. 0.15 g of dichloromethane soluble fraction was obtained.

Example 4 Preparation of Bamboo Ethyl Acetate Soluble Extract

0.2 liter of ethyl acetate was added to 0.1 liter of the water-soluble fraction obtained in Example 3, followed by mixing three to four times to obtain 0.2 liter of the water-soluble fraction and 2.5 liter of the ethyl acetate soluble fraction. The ethyl acetate layer was dried. 0.39 g of ethyl acetate soluble fraction were obtained.

Example 5 Preparation of Bamboo Butanol Soluble Extract

0.1 L of butanol was added to 0.1 L of the water-soluble fraction obtained in Example 4, followed by mixing three to four times to obtain 0.5 L of water-soluble fraction and 3 L of butanol-soluble fraction, which were then dried. g and 0.91 g of butanol soluble fraction were obtained.

Experimental Example 1. Determination of the effect of inhibiting ACE activity ( In vitro )

The inhibitory effect of ACE activity was measured according to the method of Cushman and Cheung (Cushman, DW, Cheung, HS, Biochem. Pharm. , 20 , pp1637-1647, 1971). That is, ACE 0.025 unit was added to 50 μL of 12.5 mM hippuric acid, and 100 μL of a sample dissolved in sodium borate buffer (pH 8.3) was added. At this time, a buffer was added to the blank instead of the sample. The reaction was incubated at 37 ° C. for 30 minutes, and 125 μL of 0.5 N HCl was added to stop the reaction. 1 ml of ethyl acetate was added thereto, followed by stirring for 30 seconds (vortex), followed by centrifugation at 3,000 rpm for 15 minutes to obtain 900 µL of the ethyl acetate layer as a supernatant. The supernatant was concentrated under reduced pressure, 1.5 mL of buffer was added to the residue (Hippuric acid), and the absorbance was measured at 228 nm. ACE activity inhibition rate was calculated | required by the following formula.

% Inhibition = 1 sample absorbance / untreated absorbance × 100

Table 1 and 2 show the results of measuring the inhibitory effect of the ACE activity of four kinds of domestic bamboo and extracts from each of the royal solvent. The ACE activity inhibitory effect was the highest at 64.92%, and it was higher in order of bamboo shoots, cotton rods, and stalks, and it was confirmed that the effect was significantly higher when compared to enalapril, which is currently used as an inhibitor of synthesis. there was. In addition, the extracts of the royal solvents showed the highest inhibitory effect of ACE activity of 78.44% in the butanol layer, followed by ethyl acetate, dichloromethane, water layer and hexane layer.

sample ACE inhibition rate (%) Blind porridge (10mg / ml) 61.42 King (10 mg / ml) 64.92 Cotton rod (10mg / ml) 60.46 Scoop (10mg / ml) 59.63 Enalapril (10mg / ml) 37.95

sample ACE inhibition rate (%) Hexane (10mg / ml) 68.37 Dichloromethane (10mg / ml) 70.86 Ethyl acetate (10mg / ml) 77.43 Butanol (10mg / ml) 78.44 Water (10mg / ml) 68.81 Enalipril (10mg / ml) 37.95

One example of the pharmaceutical composition containing the bamboo extract of the present invention will be described, but the present invention is not intended to limit it, but is intended to explain in detail only.

Formulation Example 1 Preparation of Powder

300 mg of the crude crude extract of Example 1

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

50 mg of the crude crude extract of Example 1

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation Example 3 Preparation of Capsule

50 mg of the crude crude extract of Example 1

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

50 mg of the crude crude extract of Example 1

Appropriate sterile distilled water for injection

pH adjuster

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

Bamboo crude extract of Example 1 100 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Formulation Example 6 Preparation of Healthy Food

Bamboo crude extract of Example 1 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15 mg

Vitamin B ₆ 0.5 mg

0.2 μg of vitamin B ₁₂

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 7 Preparation of Healthy Drink

Bamboo crude extract of Example 1 1000 mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to a conventional healthy beverage manufacturing method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

The composition containing the bamboo extract of the present invention inhibits ACE (angiotensin converting enzyme) activity and shows a consistent blood pressure lowering effect, and there is no side effect even in long-term use, so the medicine and health for the treatment of hypertension including essential hypertension Can be used in nutraceuticals.

Claims (8)

A pharmaceutical composition for the prevention and treatment of hypertension, including essential hypertension, containing bamboo crude extract as an active ingredient and a pharmaceutically acceptable carrier or excipient. The pharmaceutical composition of claim 1, wherein the crude extract is extracted with a polar solvent such as water, lower alcohol, or a mixed solvent thereof. A pharmaceutical composition for the prevention and treatment of hypertension, including essential hypertension, containing a bamboo nonpolar solvent soluble extract as an active ingredient and a pharmaceutically acceptable carrier or excipient. The pharmaceutical composition according to claim 3, wherein the nonpolar solvent soluble extract is extracted with a solvent selected from hexane, dichloromethane, ethyl acetate. The bamboo according to any one of claims 1 to 4, wherein the bamboo is selected from the group consisting of Phyllostachys bambusoides , Phyllostachys nigra var. Henonis , Phyllostachys pubescens and Sasa borealis . Phosphorus pharmaceutical composition. The pharmaceutical composition according to any one of claims 1 to 4, wherein the bamboo extract is extracted from bamboo stems or leaves. Health functional food comprising bamboo extract and food acceptable food supplement additives exhibiting a preventive effect of hypertension including essential hypertension. The health functional food according to claim 7, which is a health beverage.