KR200474541Y1 - Artificial incubator for fertilized egg - Google Patents

Abstract

The present invention relates to a poultry embryo culture incubator for a surrogate egg shell, and more particularly to a poultry embryo culture incubator for a surrogate egg shell. The present invention as described above is an incubator specialized for in vitro artificial cultivation of poultry embryos using a surrogate egg shell, and has an effect of maintaining the angle of the frame at a different angle depending on the incubation time.

Description

{Artificial incubator for fertilized egg}

The present invention relates to a poultry egg incubator for a surrogate egg shell, and more particularly to a poultry egg incubator for a surrogate egg shell.

Poultry embryos contain large amounts of egg yolk compared to mammals and are large in size. In addition, mammals progress in all stages of fertilization, initial embryogenesis and embryogenesis in the maternal body, but in poultry all processes are carried out in the egg shell except for fertilization and early embryogenesis. Therefore, in the case of poultry, an organism manipulated by an artificial in vitro culture method during gene manipulation using an embryo can be generated to an individual.

In vitro artificial cultivation using surrogate egg shells is a technique for hatching egg yolk, a fertilized egg of a poultry, into chicks using the shell of another egg. For this purpose, the upper part of the surrogate egg should be cut, the existing contents should be discarded, and the embryo of another egg should be taken and moved for the transfer of yolk with embryo. Therefore, since the upper part of the egg shell is cut into a concave bowl shape, it is common to carry out a process of culturing the upper part by a method such as sealing with a lap.

The artificial cultivation of eggs using surrogate egg shells is divided into two stages: the first stage from the first stage of hatching to 1-3 days and the third stage from 4-18 days. Secondary culture means transferring the embryos to a similar sized surrogate egg, filling the eggs completely, incubating the egg shells wrapped with the lids in a sealed state, and incubating them at 90 degrees, and the third incubation means that the second incubated embryo is about 25 grams After transferring to a surrogate egg, it means incubation without further egg white feeding and 30 days after incubation.

The embryos of chickens are characterized by the growth of blood vessels and the growth of blood vessels bound to egg shells. Specifically, the blood vessels produced during the secondary culture are not bound to the egg shell, but during the third culture, the blood vessels produced are bound to the egg shell and grow.

Although the egg yolk is larger than the fertilized egg by about 25g, the fertilized eggs come into contact with the sealed lap portion when the eggshell angle is 90 °, It is necessary to control 30 degrees.

Therefore, in the artificial hatching of chicken embryos using a surrogate egg shell, which is one of the techniques necessary for the production of transgenic chickens, the incubators disclosed in Korean Patent Publication Nos. 2010-0086379 and 2010-0096600 are eggs Since it is the incubation period optimized for hatching, it is disadvantageous that artificial hatching of chicken embryo using surrogate egg can not be performed smoothly by controlling the egg angle according to the culturing order.

Under these technical backgrounds, the present inventors have made an effort to develop an embryo culture incubator for a surrogate egg shell that can control the angulation of the egg, resulting in the completion of the present invention.

Finally, the object of the present invention is to provide a poultry embryo incubator for surrogate egg shells optimized for culturing poultry embryos for surrogate egg shells.

In order to solve the above technical problem,

According to one aspect of the present invention, a frame for embryo placement; A timer for measuring the incubation time; An angle adjuster for adjusting the angle of the frame to be maintained at 30 degrees or 90 degrees with respect to the vertical plane according to the incubation time; A temperature regulator for regulating the temperature in the incubator; And a humidity regulator for regulating the humidity in the incubator may be provided.

In one embodiment, the angle adjusting portion includes a support shaft coupled to the frame, a connection bar connected to the support shaft, a moving portion connected to the connection bar and moving on the drive shaft in accordance with rotation of the drive shaft, And an angle adjusting sensor for indicating whether the driving motor is in contact with the latch and the timer. The timer maintains the angle of the frame at 90 degrees for a period of 1 to 3 days from the start of the incubation , And the frame can be adjusted so that 30 degrees is maintained for a period from 4 days to 18 days.

The present invention as described above is an incubator specialized for in vitro artificial cultivation of poultry embryos using a surrogate egg shell, and has an effect of maintaining the angle of the frame at a different angle depending on the incubation time.

1 is a front view of a fertilized egg incubator according to the present invention.
Fig. 2 shows the inside of the angle adjusting portion 102. Fig.
3 is a side view of the fertilized egg incubator according to the present invention.
4 is a side view of the fertilized egg incubator according to the present invention.
Fig. 5 is a photograph taken from the front of the fertilized egg incubator.
6 is a front view of the incubator in which the angle of the frame is adjusted to 30 degrees by the angle adjusting unit.
7 is a photograph of the latch 203 and the angle adjusting sensors 204 to 207 inside the angle adjusting portion.
8 is a photograph showing the support shaft 105, the connecting bar 202, the moving part 201, and the drive shaft 200 in the angle adjusting part.
FIG. 9 is a photograph of an incubator in which the angle of the frame is adjusted by the angle adjusting unit.
10 is a photograph of an incubator in which the angle of the frame is adjusted by the angle adjusting unit.

The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. It should be understood, however, that the appended claims are not intended to limit the invention to the particular embodiments, but to include all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a component, or a combination thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

According to one aspect of the present invention, a frame (101) for placing an embryo; A timer 209 for measuring the incubation time; An angle adjusting unit (102) for adjusting the angle of the frame according to the incubation time so as to maintain 30 degrees or 90 degrees with respect to the vertical plane; A temperature regulator 103 for regulating the temperature in the incubator; And a humidity regulator 104 for regulating the humidity in the incubator can be provided for a surrogate egg shell poultry embryo culture incubator.

According to one embodiment, the angle adjusting unit 102 includes a support shaft 105 coupled to the frame, a connection bar 202 connecting the support shaft and the drive shaft, The frame 209 is composed of a moving part 201, a latch 203 provided at one side of the connecting bar and angle adjusting sensors 204, 205, 206 and 207, You can maintain 90 degrees during the first 1-3 days and 30 degrees during the 4th - 18th days.

The frame 101 coupled with the support shaft 105 may further be equipped with a full-length adapter in which an embryo is placed. The catheter adapter may exhibit various shapes depending on the purpose of the culture, the number of embryos or other specificities.

In the adapter according to one embodiment of the present invention, a plurality of seats to which an embryo can be placed are arranged in a row, and the seats are arranged such that the embryos do not contact each other.

The incubator according to the present invention measures the incubation time with a timer. The incubator according to the present invention is divided into the incubation time of the embryo as measured by the timer, that is, the second incubation period (1 to 3 days) and the third incubation period (4 to 18 days) By adjusting the angle of the frame 101 according to the incubator, the angular angle of rotation is changed.

The angle of the egg is adjusted to be 30 degrees or 90 degrees by the angle adjuster 102 so that even when the frame is 90 degrees with respect to the vertical line to the ground, It is preferable that a seating groove is formed.

Embryos of chickens grow and blood vessels are formed. The blood vessels that are produced in this way are characterized by being bound to egg shells and growing. The artificial culture stage of the embryo is divided into a secondary culture corresponding to 1-3 days and a tertiary culture corresponding to 4-18 days. The blood vessels generated in the secondary culture stage are not combined with eggs, It is possible to keep.

The angular angle of 90 degrees is advantageous in that the air chamber is directed upward when the embryo generator is generated, thereby improving the incidence of breathed breathing. However, since the blood vessels produced in the third culture step are bound to the egg shell, when the egg shell angle is 90 degrees, the upper part of the egg shell is removed, and the sealed wrapping part is in contact with blood vessels, There is a problem in that the occurrence is reduced.

Therefore, in the tertiary culture step, the egg angle is maintained at 30 degrees, thereby preventing the embryo from reaching the lap portion and ensuring the ease of hatching of the wavenumber.

According to the present invention, the embryo angles of the embryo can be set to 90 degrees and 30 degrees, respectively, in one incubator, and the egg time interval can be varied from a minimum of 10 minutes to a maximum of 48 hours.

3 and 4, the support shaft 105 moves the frame 101 up and down to adjust the angle of full-angle, and the up and down movement of the support shaft 105 is performed by the drive shaft 200 .

The drive shaft 200 is connected to the support shaft 105 through a connection bar 202. On the other side of the connecting bar is a latch 203. The movable part 201 moves upward and downward by the forward rotation and the reverse rotation of the drive shaft 200 and the latch 203 located at one side of the connection bar also moves up and down. The latch is in contact with the angular position sensors 204, 205, 206, 207 and can maintain the frame within the incubator at a desired angle.

The principle of controlling the angle of the frame is as follows. That is, when the latch which moves up and down according to the rotation of the drive shaft contacts the angle adjustment sensor of the undesired angle, the latch is passed as it is, and when the angle adjustment sensor of the desired angle is contacted, Signal. The connecting bar conveys the up and down motion as it is to the supporting shaft, and the frame inside the incubator forms an angle corresponding thereto.

The angle adjusting sensor includes first to fourth angle adjusting sensors.

The first angle-regulating sensor 204 and the fourth angle-regulating sensor 207 are arranged such that the angle of warp angle is 90 degrees, the angle of the second angle-regulating sensor 205 and the angle of the third angle- 203).

The temperature regulating unit 103 and the humidity regulating unit 104 are provided in the incubator according to the present invention as means for maintaining an environment suitable for culture of the fertilized eggs.

Here, the temperature controller 103 can circulate the air inside and outside the incubator through the air-jacket heating method and the inner rear fan, and the air inside the incubator can be circulated through the air jacket heating system So that the internal temperature difference can be minimized. The humidity controller 104 can be appropriately maintained in the water filled in the evaporator provided at the lower end of the incubator and the amount of water evaporated can be adjusted by controlling the flow of air circulated by the temperature controller 103.

According to another aspect of the present invention, a poultry embryo culture incubator for a surrogate egg shell in the form of a single tank without the addition of the bottom of the incubator can be provided.

In the incubator, the sides and the bottom of the incubator are seamlessly formed, so that the phenomenon of foreign matter sticking to the gap is minimized. In vitro artificial cultivation using surrogate egg shells cuts the upper part of the surrogate egg, discards the original contents, and then transfers the embryos of the other eggs to be sealed with a lap.

Here, even if the upper part of the cut egg is sealed with a lap, there is a high possibility of being infected by an infectious substance such as bacteria or virus, and in case of infection, a great disadvantage is incurred in producing a desired transgenic chicken. Therefore, in the artificial in vitro culture using surrogate egg shell, it is essential to maintain the inside of the incubator in order to protect the embryos in the embryo, which is likely to be immunologically infected. Accordingly, , It is possible to minimize the possibility of contamination which may occur due to the foreign substance in the joint.

The present invention as described above is an incubator specialized for in vitro artificial cultivation of poultry embryos using a surrogate egg shell, and has an effect of maintaining the angle of the frame at a different angle depending on the incubation time.

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the invention as defined by the appended claims. Therefore, the true technical protection scope of this invention should be determined by the following utility model registration claim range.

101: Frame 102: Angle adjusting section
103: Temperature control unit 104: Humidity control unit
105: support shaft 200: drive shaft
201: moving part 202: connecting bar
203: Pawl 204: 1st angle adjustment sensor
205: second angle adjusting sensor 206: third angle adjusting sensor
207: fourth angle adjusting sensor 208: drive motor
209: Timer

Claims (2)

A frame to place an embryo;
A timer for measuring the incubation time;
A connecting bar connected to the supporting shaft, a moving part connected to the connecting bar and moving on the driving shaft in accordance with rotation of the driving shaft, a latch provided on one side of the connecting bar, Wherein the timer maintains the angle of the frame at 90 degrees for a period of one to three days from the beginning of the incubation to the vertical plane, and for a period of from 4 days to 18 days Wherein the frame adjusts the frame to maintain the angle of 30 degrees.
A temperature regulator for regulating the temperature in the incubator; And
A poultry embryo incubator for a surrogate egg shell containing a humidity regulator for regulating the humidity in the incubator.
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