KR20040099936A - Antioxidant composition comprising quercetin and vitamin C and functional food containing the same - Google Patents

Abstract

PURPOSE: An antioxidant composition containing quercetin and vitamin C is provided. It is applied to milk products, beverages, health supplementary foods, gum, cosmetics, medical bands or the like to give an antioxidant function. Therefore, it is helpful for improving health and preventing aging. It also can be used as an inhibitor and a therapeutic agent for pesticide poisoning or diabetic microvascular complications. CONSTITUTION: The antioxidant composition contains 0.1 to 99.99% by weight of quercetin and 0.01 to 99.9% by weight of vitamin C. The quercetin is prepared by distilling the acetic acid extract of onion under reduced pressure, dissolving the extract in ethyl acetate and then followed by column chromatography on silica gel. The dairy product having an antioxidant function is obtained by adding 0.01 to 50% by weight of the antioxidant composition. The dairy product is milk, yogurt, cheese, a lactic acid fermented product. The health supplementary food is obtained by mixing 5 to 77% by weight of the antioxidant composition, 3 to 75% by weight of ginseng powder and its extract, red ginseng powder and its extract, or mushroom powder and its extract.

Description

Antioxidant composition comprising quercetin and vitamin C and functional food containing the same

The present invention relates to an antioxidant composition containing quercetin and vitamin C and a functional food containing the same.

Recently, with the development of genetic engineering, the dream of disease-free long life is coming true for modern people. Therefore, more than ever, health-related anti-aging measures have been proposed. There are many factors that promote aging, but active oxygen species (ROS) are one of the important factors. Therefore, there is an increasing interest in antioxidants (free radical scavengers) that suppress the formation of active oxygen systems or remove the formed active oxygen systems.

Interest in free radicals was raised by D. Harman in the mid-1950s, which has recently gained more attention in the industry and in academic societies. Free radicals are present in the human body for a number of reasons. For example, free radicals are involved in the phagocytosis of leukocytes, the electron transport system during the production of ATP in mitochondria, the action of myeloperoxide, tobacco, and normal metabolic processes. , Stress, pollutants, bacteria, etc.

These free radicals destroy cells, cut off connective tissue in the dermis, or cause cross-linking, which can lead to wrinkles, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, acne, diabetes complications, and atherosclerosis. Generates.

The body contains amino acids containing superoxide dismutase, catalase, glutathione peroxidase, vitamin E, vitamin C, and other free or thiol groups, which are antioxidants. (cysteine, methionine), but if the amount of free radicals formed exceeds the antioxidant capacity of the individual, or the antioxidant capacity is weakened by age, pollution, ultraviolet rays, stress, etc., the antioxidant system is inactivated and the free radical gradually increases. Done.

These free radicals combine with peroxide groups in the body to make harmful substances called lipid peroxides. About 80% or more of human diseases are caused by free radical oxidation, and aging is known to be due to the oxidation of free radicals. Aging is mainly caused by the peroxidation of these lipids, and lipid peroxide acts on blood vessels and causes various adult diseases such as atherosclerosis and blood clots.

On the other hand, many toxic substances cause free radicals. Representatives are pesticides such as herbicides or insecticides. As modern people consume vegetables, fruits and other foods, even in small amounts, they are forced to consume pesticides. Therefore, there is an urgent need for measures to remove the free radicals formed. In particular, Paraquat is the most commonly used herbicide in the world, and when it is ingested in a large amount, it is known to generate large amounts of free radicals in the human body and destroy cell walls and protoplasts of human organs. Injury of major organs such as liver and liver can be easily improved, but only lung damage is rapidly deteriorated within a few days, eventually dying of hypoxia. As such, paraquet is a highly toxic pesticide that is fatal when absorbed by the human body even in small amounts, causing many victims. Therefore, there is an urgent need for methods and means for detoxification of paraquel addicts as well as the general public who can not only consume a small amount of food containing pesticides.

In addition, even minimal pesticides form free radicals in the human body, leading to circulatory disease (Am J Hypertens 11: 1480-1485, Atherosclerosis 138: 375-381, 1998), kidney disease (Free Radic Res 29: 273-281, 1998), Severe exercise (Med Sci Sports Exer 30: 1603-1607, 1998), sepsis (Free Radic Biol Med 23: 768-774, 1997), hepatic dialysis converts (Free Radic Res 29: 367-376, 1998), Diabetes (Atherosclerosis 137: S61-S64, 1998) and hyperlipidemia (Br J Clin Pharacol 44: 94-97, 1997) are known to have a significant effect on the disease, so it is urgent to prepare countermeasures.

Therefore, in recent years, a lot of research has been conducted to develop an antioxidant that is safer to human body, cheaper, and has excellent antioxidant power. In general, antioxidants used in or under clinical research include melatonin, alpha-tolopherol, and vitamin C in fruits, vegetables, and red wine, and vitamin C is relatively injectable and water-soluble. There is an advantage that a large amount can be safely injected into the human body in a short period of time. In addition to its use as an antioxidant, vitamin C is involved in the metabolism of anticancer, folate, amine, glucose, iron, cyclic nucleotides, cholesterol, and many other drugs.In addition, it increases the function of leukocytes, and also enhances the synthesis of interferon and carnitine. It is known to promote and to reduce the amount of paraquet in the kidney tissue by interfering with the paraquet accumulation in the kidney.

The best way to detoxify paraquet herbicides is to administer vitamin C to inhibit the formation of free radicals before destroying the cell membrane in the body.However, when ingested in excess, side effects such as nausea, vomiting, and diarrhea may occur. Digestive symptoms may occur and urinary oxalate excretion may increase, or kidney stones or hemolysis may occur. Nevertheless, to date, excessive amounts of vitamin C have been injected into the human body to treat paraquel intoxication. However, vitamin C is also known to play a role in increasing harmful oxygen in the presence of metal ions such as divalent iron ions released from damaged cells [Free Radic Res 1998 Jan; 28 (1); 93-107]. Therefore, there is an urgent need for a new approach to improve the antioxidant effects of vitamin C.

On the other hand, quercetin (quercetin) is a component contained in the onion extract is known in the art as a free radical scavenger or antioxidant, but there is no specific example used to treat the poisoning to pesticides.

Recently, the development of beverages using onion extract (Korean Patent Publication No. 2003-24040, 2001-7675) and slimming cosmetics including onion extract (Korean Patent Publication No. 1999-55308) have been made. There is a lack of research on sex compositions.

Therefore, the present inventors found that there is a synergistic effect as an antioxidant when the quercetin and the vitamin C are administered together in a general person who is addicted to a pesticide such as paraquet or consumed even a small amount daily. The present invention has been reached.

That is, both quercetin and vitamin C are known in the art as a free radical scavenger or antioxidant, but when used together, it has been found that there is a synergy as an antioxidant without side effects than when each is used. When the composition is applied to beverages, foods, gums, cosmetics, medical bands, etc., antioxidant properties can be enhanced, and it is also found that it can be applied as an agent for treating pesticide poisoning and diabetic microblood and complications.

The present invention provides an antioxidant composition containing quercetin and vitamin C.

Another object of the present invention is to provide a beverage, food, gum, cosmetics, medical bands and the like containing a certain amount of the antioxidant composition.

Still another object of the present invention is to provide the antioxidant composition as a therapeutic agent for pesticide poisoning and diabetic microvascular complications.

1 is a confocal microscopic photograph of cells treated according to the experiment of Example 4 of the present invention (100 times magnification)

FIG. 2 is a fluorescence data graph of the micrograph of FIG.

Figure 3 is a graph comparing the degree of cell damage according to the generation of harmful oxygen treated according to the experiment of Example 4 of the present invention.

The present invention is characterized by an antioxidant composition containing 0.1 to 99.99% by weight of quercetin and 0.01 to 99.9% by weight of vitamin C.

The present invention, the antioxidant composition, dairy products such as milk, yogurt, cheese, health supplements such as ginseng, red ginseng, mushrooms or extracts thereof, juices, carbonated beverages, traditional teas, beverage products such as other beverages and gum compositions It is characterized by a dietary supplement containing 0.01-50% by weight.

The present invention is characterized by a functional cosmetic composition and a medical band composition in which the antioxidant composition is contained in an amount of 0.01-20% by weight in a conventional cosmetic composition or a conventional medical band composition.

The present invention is characterized by a therapeutic agent for pesticide poisoning and diabetic microvascular complications comprising the antioxidant composition and a pharmaceutically acceptable carrier.

Hereinafter, the present invention will be described in detail.

In the present invention, a quercetin was used as a commercial product or extracted from onion, and the extraction method was performed by silica gel column chromatography by dissolving the acetic acid extract of onion in ethyl acetate after distillation under reduced pressure, but normal hexane. Quecetin was obtained using a mixed solvent having a ratio of 1: 2 and ethyl acetate. It was confirmed that green needles were precipitated in the quercetin solution obtained after the silica gel column chromatography, and the mixture was slowly and slowly concentrated at room temperature. This decision was then recrystallized to obtain quercetin.

In the present invention, vitamin C was purchased from a commercially available product, such as Handok Pharmaceutical Co., Ltd. (molecular weight 176.1).

The quercetin and vitamin C are mixed in a conventional manner to prepare an antioxidant composition containing 0.1 to 99.99% by weight of quercetin and 0.01 to 99.9% by weight of vitamin C.

The antioxidant composition thus obtained is added to the dairy products such as milk, yogurt, cheese or other lactic acid bacteria fermentation products or fruit juices, carbonated beverages, traditional tea beverages, and other palates in 0.01 to 50% by weight of dairy products or beverages having an antioxidant function Can be prepared.

And 5-77% by weight of the antioxidant composition thus obtained is 3 to 75% by weight of health supplements, such as ginseng powder and extract, red ginseng powder and extract or mushroom powder and extracts, other additives (for example, sweeteners and flavors) 1 It may be mixed with -20% by weight to prepare a dietary supplement.

In addition, 0.01 to 20% by weight of the antioxidant composition may be added to the cosmetic compound. Methods of making cosmetic compounds are known to those skilled in the art. The cosmetic composition may take the form suitable for topical use, including, for example, skin, lotion, cream, gel, pack or solid, ie stick form. Cosmetic compositions contain an antioxidant compound of the present invention in an amount that depends on the ratio of formulation for a particular use and the intended use of the composition. For example, when the formulation for a specific use is a cosmetic pack, the antioxidant compound of the present invention is 0.01 to 20% by weight of the composition, preferably 0.1 to 10% by weight can be provided to have an antioxidant effect.

By containing 0.01 to 20% by weight of the antioxidant composition in a conventional medical band composition can be prepared a functional medical band composition excellent in antioxidant function.

The antioxidant composition can be prepared in a gum having an antioxidant effect by containing 0.01 to 50% by weight in a conventional gum composition.

By adding a pharmaceutically effective amount of such an antioxidant composition to a pharmaceutically acceptable carrier, it can also be used as a therapeutic agent for pesticide poisoning and diabetic microvascular complications. In clinical use, the antioxidant composition of the present invention may be combined with a conventional carrier in the pharmaceutical field for oral administration of a conventional agent in the pharmaceutical field, for example, tablets, capsules, crokies, solutions, suspensions, and the like. Formulations; It may be formulated into a variety of preparations, such as injectable solutions or suspensions, or injectable preparations in the form of ready-to-use injectable dry powders that can be prepared and used as injectable distilled water at the time of injection.

In addition, when the antioxidant composition of the present invention is used as a health supplement, it can be used in combination with carriers conventional in the field of health supplement products.

Hereinafter, the present invention will be described through the following examples, but the present invention is not limited thereto.

Example 1 Extraction and Separation of Quercetin

Finely chopped 200 g of onion, distilled under reduced pressure by adding 500 ml of acetic acid, and then dissolving it in 500 ml of ethyl acetate. Then, silica gel column chromatography was used, and the mixed solvent of normal hexane and ethyl acetate in a ratio of 1: 2 was used. Obtained quercetin. It was confirmed that green needles were precipitated in the quercetin solution obtained after the silica gel column chromatography, and the mixture was slowly and slowly concentrated at room temperature. This crystal was continuously recrystallized to obtain 110 mg of quercetin.

Example 2: Preparation of Antioxidant Composition

Vitamin C (molecular weight 176.1 Handok Pharmaceutical Co., Ltd.) and quercetin (molecular weight 338.3) extracted in Example 1 were prescribed in the composition shown in Table 1 below.

group Quercetin (mg / kg / IV) Vitamin (mg / kg / IV) ABC 000 010100 DEFGHI 101010100100100 010100010100

Example 3: Synergistic Effects of Antioxidant Compositions

90 rats (rat, 200g) were divided into 9 groups of 10 rats as shown in Table 1 above, and 25 mg / kg of paraquests were injected intraperitoneally, respectively, and then administered to each group with the composition shown in Table 1 above. The results of recording the number of mice sacrificed by date from group A to I are shown in Table 2 below.

group injection 1 day 2 days 3 days 4 days 5 days 6 days 7 days 8th 9th 10 days 11th A 6 2 2 B 6 4 C 4 6 D 6 4 E 2 4 4 F 2 4 2 2 G 2 4 4 H 2 2 4 2 I 2 2 2 2 2

From Table 2, the group not administered vitamin C and quercetin at all (group A) began to die from the 4th day after paraquet poisoning and sacrificed at the 6th day, but the group administered only vitamin C (B, Group C) and the quercetin-only group (D, G group) can be seen to be effective in treating addiction even when each of the sacrifices administered from 5 days. However, in the group of vitamin C and quercetin (groups E, F, H, and I), the number of mice sacrificed earlier was lower than that in each group, especially in group I. By confirming that it can be delayed by days, it can be confirmed that the administration of vitamin C and quercetin together has a synergistic effect compared to the administration of each to treat addiction.

Example 4 Antioxidant Activity Measurement

After administering 500 μM of paraquet intraperitoneally to rats, the results of measuring the concentrations of various concentrations of quercetin and vitamin C were administered to the rats.

ROS was measured by confocal microscopy of cells in the following manner (Cell Signal 1999; 11 (9); 67783). Cells were exposed to the conditions described above, incubated in serum-free medium for 24 hours on round coverslips, then stabilized for about 1 hour and again in 2 g / ml Lysophosphatidic acid (LPA) for 30 minutes. After exposure, fix for 20 minutes in phosphate buffer containing 3.7% (v / v) formaldehyde. Stain with 0.165 M rhodamine phalloidin for 1 hour at room temperature. These cells are treated with gelvatol and read by laser scanning confocal microscopy (LSM 410, Carl Zeiss). Specimens are stimulated with a 543 nm HeNe laser and images are acquired through a long pass 580 nm filter.

1 is a confocal microscopic picture of cells treated according to the above experiments, and when quercetin and vitamin C were administered together, it can be seen that cell damage was significantly healed.

FIG. 2 is a graph of their fluorescence data. At concentrations between 0.01 μM and 100 μM of quercetin and between 1 μM and 10 μM of vitamin C, free radicals formed by paraquet are inhibited in proportion to their respective concentrations. At this time, when quercetin 0.01μM and vitamin C 1μM treatment together, the active oxygen inhibitory ability is higher than when treated separately. The same is true with quercetin 0.1 μM and vitamin C 10 μM.

As shown in FIG. 3, Paraquete 500 μM was treated to express free radicals, and quercetin and vitamin C were simultaneously treated in various concentrations, and both were treated simultaneously compared to the cell group treated with quercetin and vitamin C. It can be seen that the production of reactive oxygen is significantly suppressed under the conditions. That is, as a result of measuring the ROS by administering the quercetin and vitamin C-containing composition according to the present invention, it can be seen that the excellent antioxidant effect is exhibited due to the expression of synergistic effects even with quercetin 0.1μM and vitamin C 10μM.

Example 5: Functional Dairy

In 200 ml of milk, 1.5 wt% of the Group I antioxidant composition of Example 2 was added and mixed uniformly to prepare a milk product according to the present invention.

Example 6: Functional Beverage Product

0.5 wt% of Group I antioxidant composition of Example 2 was added to 180 ml of fruit juice, and the mixture was uniformly mixed to prepare a functional beverage product according to the present invention.

Example 7: Health Supplement

30% by weight of Group I antioxidant composition of Example 2 and 20% by weight of sweetener and flavourant were added to 50% by weight of ginseng powder and uniformly mixed to prepare a dietary supplement according to the present invention.

Example 8: Chewing Gum

1 wt% of Group I antioxidant composition of Example 2 was mixed with 25% by weight of gum base, 39% by weight of sugar, 21% by weight of sugar, 7% by weight of starch syrup and 7% by weight of spice according to the present invention Was prepared.

Example 9 Ocher Packs

1 wt% of the Group I antioxidant composition of Example 2 was made up by 94.8 wt% of ocher, 0.2 wt% of rosewater, 0.4 wt% of collagen, 2.0 wt% of allatoin, 0.5 wt% of aloe, 0.2 wt% of ceramide, 0.2 wt% of arbutin, Tocopheral acetate 0.2% by weight, squalane 0.2% by weight, 0.2% by weight of witch harrell and 0.1% by weight of biofactide was prepared ocher pack having an antioxidant effect according to the present invention.

Example 10 Medical Bands

After applying the colorless transparent absorbent adhesive material to the gauze, 0.5% by weight of acrinoline (pharmaceutical), 35.1% by weight of amimobenzoic acid, 5.9% by weight of lidocaine hydrochloride (pharmaceutical), zinc oxide per 10cm × 10cm A powder was added to the medical band prepared by mixing 58.0% by weight, 0.5% by weight of Group I antioxidant composition of Example 2.

Example 11: Pesticide Addiction Therapeutics

0.5 wt% of Group I antioxidant composition of Example 2 was mixed with a conventional pharmacological composition of conventional glucose, amino acid, and physiological saline to prepare an injection.

Example 12 Diabetic Microvascular Complications

0.3 wt% of Group I antioxidant composition of Example 2 was mixed with a conventional pharmacological composition of conventional glucose, amino acid, and physiological saline to prepare an injection.

As described above, the quercetin and vitamin C-containing antioxidant composition according to the present invention is applied to dairy products, beverages, dietary supplements, gums, cosmetics, medical bands, etc. to improve health and prevent aging due to the provision of antioxidant functions. In addition, it can be useful as an inhibitor and treatment of pesticide poisoning or diabetic microvascular complications due to its antioxidant function that antagonizes oxidative stress, which is a cause of pesticide poisoning or diabetic microvascular complications. .

Claims (13)

An antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C. The antioxidant composition according to claim 1, wherein the quercetin is obtained by performing distillation under reduced pressure on acetic acid extract of onion grind and dissolving it in ethyl acetate and performing silica gel column chromatography. A dairy product having an antioxidant function, prepared by adding 0.01-50% by weight of an antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C to dairy products. 4. The dairy product of claim 3 wherein the dairy product is milk, yogurt, cheese, or other lactic acid bacteria fermentation product. A beverage having an antioxidant function, prepared by adding 0.01-50% by weight of an antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C. 6. The beverage of claim 5 wherein the beverage is fruit juice, carbonated beverage, traditional tea beverage, or other palate. Antioxidant function prepared by mixing 5-77% by weight of antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C with 3-75% by weight of dietary supplements and 1-20% by weight of other additives. Health supplements having. The dietary supplement of claim 7, wherein the dietary supplement is ginseng powder and extract, red ginseng powder and extract or mushroom powder and extract. A medical band composition having an antioxidant function prepared by mixing 0.01-20% by weight of an antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C with a conventional medical band composition. A cosmetic composition having an antioxidant function prepared by mixing 0.01-20 wt% of an antioxidant composition containing 0.1-99.99 wt% of quercetin and 0.01-99.9 wt% of vitamin C with a conventional cosmetic composition. A gum composition having an antioxidant function prepared by mixing 0.01-50% by weight of an antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C with a conventional gum composition. A pharmaceutical composition for treating pesticide poisoning, comprising a pharmaceutically effective amount of an antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C and a pharmaceutically acceptable carrier. A therapeutic composition for diabetic microvascular complications comprising a pharmaceutically effective amount of an antioxidant composition containing 0.1-99.99% by weight of quercetin and 0.01-99.9% by weight of vitamin C and a pharmaceutically acceptable carrier.