KR20040089258A - Composition for treating obesity and constipation containing extract of pine needle, green tea and black tea as an active ingredient - Google Patents

Abstract

PURPOSE: Provided is a composition for treating obesity and constipation which contains extracts of pine needle, green tea and black tea as an active ingredient. The increases obesity inhibition effect, reduces body weight and body fat, and increase quantity of feces. Therefore, it has excellent effects on prevention and treatment of obesity and constipation. CONSTITUTION: The pine needle, green tea and black tea are respectively extracted by using hot water of predetermined temperature. Each of the extracts are mixed in a mixing ratio of 1-4:1-4:1-4. The composition for prevention and treatment of obesity and constipation contains the mixed extracts as an active ingredient.

Description

Composition for treating obesity and constipation containing extract of pine needle, green tea and black tea as an active ingredient

The present invention relates to a composition for the treatment of obesity and constipation containing pine needles, green tea and black tea extract as an active ingredient, and more particularly, by the composition of a mixed ratio of hot water extracts of pine needles, green tea and black tea at a certain ratio The present invention relates to a composition as a dietary supplement exhibiting an excellent effect on the treatment of constipation, as well as an obesity inhibitory effect when taken over a period of time.

In general, obesity is a type of disease in which energy left over is converted into triglycerides and accumulated mainly in the adipocytes of the abdomen and subcutaneous fat tissue. It is a complex syndrome caused by a variety of causes, including nutritional, environmental, and social factors. In other words, when the calorie intake exceeds the energy consumption, the remaining energy is converted into fat (Fat) and stored in fat cells, which eventually causes obesity.

Although obesity itself is a disease that causes daily life problems, there is a serious problem that obesity causes various complications rather than the meaning of the disease itself. Obesity causes hyperlipidemia, an increase in the amount of cholesterol and / or triglycerides in the blood, leading to fatal diseases such as hypertension, cardiovascular disease, and cerebral vascular accident Triglyceride accumulation in peripheral tissues (Kelley & Mandarino, A Reexamination. Diabetes , 49: 677-683 (2000)) and abdominal adipose tissue (Kelley et al., Am. J. Physiol., 278: E941 E948 (2000)) may cause insulin resistance, leading to type 2 diabetes. This can be easily seen by the result of 40 times higher incidence of diabetes in obese people compared to normal people.

In addition, obesity causes a number of complications such as arthritis, respiratory dysfunction, infertility and menstrual irregularities, malignant tumors, so long-term management and treatment are absolutely necessary.

As described above, in order to suppress obesity, which is a source of all kinds of diseases, it is necessary to continuously reduce fat accumulation in the body by reducing food intake and increasing energy consumption. However, although it is well known that suppressed diets can induce weight loss, in many cases diet alone will not lead to long term weight loss, as dietary intake does not effectively reduce food cravings unless accompanied by the intake of chemical appetite reducers. It does not promote.

Therefore, increasing energy expenditure with steady and sustained exercise, rather than relying on reduced food intake, is more effective in suppressing obesity. However, due to the nature of busy social activities, it is difficult to continually exercise, so the next best option is to take obesity by taking drugs and supplements that have anti-obesity effects.

Due to these social trends today, the development of therapeutics that can prevent and treat obesity is being actively promoted. Currently, there are two internationally recognized therapeutic agents: appetite suppressant reductil (named sibutramine) and xenical (named orlistat) which inhibits fat digestion. Both drugs are difficult or dangerous for the general consumer to take for the purpose of preventing obesity because of both large and small side effects as medicines. Therefore, there is an urgent need to develop a new health food using plant materials and substances as an anti-obesity preventive agent that is harmless to the human body without fear of side effects caused by chemically synthesized drugs.

On the other hand, constipation generally depends a lot on individual's dietary habits and behavioral styles, but the frequency of the constipation is increasing due to the lack of dietary habits and exercise of modern people who like simple foods such as fast food due to changes in living environment. .

Drugs that temporarily increase the movement of the intestine as a drug for the treatment of constipation, but due to the increase in artificial intestinal movement can cause more serious side effects due to the relatively reduced intestinal movement. Therefore, in order to treat constipation more effectively, it is desirable to increase the amount of stool in such a way as to increase the intake of dietary fiber to activate the intestinal movement.

The present invention has been made in view of the above-mentioned problems, and its object is to extract specific ingredients of natural foods and produce them at a predetermined ratio, thereby suppressing obesity or constipation symptoms without any adverse effects on the human body. And it is to provide a composition for the treatment of obesity and constipation containing pine needles, green tea and black tea extract as an active ingredient that can exhibit an excellent effect in the prevention.

Obesity and constipation treatment composition containing pine needles, green tea and black tea extract according to the present invention for achieving the above object is a mixed ratio of pine needle extract and green tea extract and black tea extract extracted by hot water at a predetermined temperature, respectively Is characterized in that it is mixed in a weight ratio of 1 to 4: 1 to 4: 1 to 4.

Here, the hot water is preferably used by heating the distilled water to 70 ~ 90 ℃ for a predetermined time.

1a to 1e are graphs showing the degree of inhibition of weight gain according to the dietary content of the pine needles, green tea and black tea extracts alone or mixed administration for 5 weeks, respectively.

Figure 1a is a graph showing the degree of weight increase inhibition according to the dietary content of pine needle extract,

Figure 1b is a graph showing the degree of weight increase inhibition according to the dietary content of green tea extract,

Figure 1c is a graph showing the degree of weight increase inhibition according to the dietary content of black tea extract,

Figure 1d is a graph showing the degree of weight gain inhibition according to the dietary content of pine needles, green tea and black tea extract mixture,

Figure 1e is a graph comparing the effect of inhibiting weight gain according to the dietary content of the administration of pine needles, green tea and black tea extracts alone and the mixture of each of the extracts.

2a to 2e are graphs showing the degree of abdominal fat inhibition according to the dietary content of the pine needles, green tea and black tea extracts alone or mixed administration for 5 weeks, respectively,

Figure 2a is a graph showing the degree of abdominal fat inhibition according to the dietary content of pine needle extract,

2b is a graph showing the degree of abdominal fat inhibition according to the dietary content of green tea extract,

Figure 2c is a graph showing the degree of abdominal fat inhibition according to the dietary content of black tea extract,

Figure 2d is a graph showing the degree of abdominal fat inhibition according to the dietary content of pine needles, green tea and black tea extract mixture,

Figure 2e is a graph comparing the effect of inhibiting abdominal fat according to the dietary content of the pine needles, green tea and black tea extracts alone administration of the mixture of each extract.

3a to 3e are graphs showing the increase in variance according to the dietary content of the pine needle, green tea and black tea extracts alone or mixed administration for 5 weeks, respectively,

Figure 3a is a graph showing the increase in variance according to the dietary content of pine needle extract,

Figure 3b is a graph showing the increase in variance according to the dietary content of green tea extract,

Figure 3c is a graph showing the increase in variance according to the dietary content of black tea extract,

Figure 3d is a graph showing the increase in variance according to the dietary content of pine needles, green tea and black tea extract mixture,

Figure 3e is a graph comparing the effect of increasing the variance according to the dietary content of the administration of pine needles, green tea and black tea extracts alone and the mixture of each of the extracts.

In the present invention, while extracting a specific component from pine needles, green tea and black tea, in order to ensure the physical properties of each of these extracts, pine needles, green tea and black tea were extracted using hot water at 70 ~ 90 ℃. The extraction method may use a conventional method known in the art.

In the composition for treating obesity and constipation of the present invention, a mixture ratio of pine needle extract, green tea extract and black tea extract is mixed in a weight ratio of 1 to 4: 1 to 4: 1 to 4. In particular, when the mixing ratio of pine needles, green tea, and black tea extract is 2: 1: 1, it is preferable because an ideal obesity inhibitory effect can be obtained more than the effect exhibited by each herbal extract.

In addition, the efficacy of the composition according to the present invention can be demonstrated through the following experiment. That is, the normal carbohydrate diet (similar to carbohydrates: 59%, protein: 29%, and fat: 12%), similar to the carbohydrate diet of Koreans, may be mixed or mixed with each hot water extract of pine needles, green tea, and black tea, which constitute the components of the present invention. Samples prepared at concentrations of 0.5, 1, 2, 4 and 8% by weight were prepared and administered to SPF (Specific Pathogen Free) Sprague-Dawley rats for 5 weeks. Afterwards, body weight, fat mass, dietary intake, and variance were measured. At this time, the statistical method for determining the significance of the differences between the two groups was Student's T-test between the two groups, and ANOVA for the multilateral comparison of three or more groups, and the significance level was set to p <0.05 or less.

Through these experiments, natural extracts such as pine needles, green tea, and black tea extracts were mixed with normal diets at a concentration of 1, 2, 4, and 8% by weight, respectively, and the mixed extracts obtained by mixing and extracting 2: 1: 1 After each sample was administered to the mice, the effects on the weight gain and abdominal fat mass of the mice were examined. As a result, pine needle extracts contained 1, 2, 4, 8% by weight, green tea and black tea extracts 1, 2, Body weight and abdominal fat decreased significantly in the 4% by weight concentration group. In addition, the mixed extract group mixed with pine needles, green tea and black tea extract 2: 1: 1 in the 1, 2, 4, 8% by weight group compared to the extract of pine needles, green tea and black tea alone when compared to the same amount of anti-obesity The effect, namely body weight and abdominal fat, was decreased more than the control group. In addition, the variance of the mice was also increased in the mixed extract group compared to the control group was shown to be effective in improving constipation.

The composition for the treatment of obesity and constipation according to the present invention may contain pine needles, green tea and black tea extract or powder as an active ingredient as described above, and a pharmaceutically acceptable carrier according to a pharmaceutical preparation method known in the art. It can also be added.

Examples of the carrier include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose and polyvinyl. Pyrrolidone, water, methylhydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate, mineral oil, etc. are mentioned.

In addition, the composition of the present invention may further include a filler, an anticoagulant, a lubricant, a humectant, a perfume, an emulsifier, or a preservative. Alternatively, known fatty acid metabolites may be added. Examples of the fatty acid metabolism promoters include L-carnitine, HCA (Hydroxy Citric Acid), and the like.

In addition, the composition of the present invention is found to be clinically used for the treatment of obesity and constipation. For example, in the treatment of obesity, diabetes, hyperlipidemia, fatty liver and constipation according to the composition of the present invention, the effective amount of the pine needle, green tea and black tea extract mixture is 1 to 500 mg / kg (powder is 5 to 2500 mg / kg), preferably Is 5-50 mg / kg (the powder is 25-250 mg / kg). However, the dosage may be determined in consideration of various factors such as the severity of obesity and constipation, the condition of the patient, the age.

Hereinafter, preferred embodiments for preparing a composition for treating obesity and constipation containing pine needles, green tea and black tea extract according to the present invention as an active ingredient will be described in detail with reference to the accompanying drawings. However, the following examples are merely to illustrate the present invention, the content of the present invention is not limited only by the following examples.

Example 1 Preparation of Hot Water Extract

20 g of distilled water was added to 1000 g of pine needles, green tea, and black tea leaves, and the extract was heated twice at 120 ° C. for 120 minutes. The leaves were removed, filtered, and evaporated to dryness using a rotary vacuum concentrator. Get a hurry.

Example 2 Weight Loss Effect Experiment of Rat

(1) Weight loss effect of pine needles, green tea and black tea extract:

Sprague-Dawley rats (approximately 50 grams) of carbohydrate diet group (Rat Chow, containing 59% carbs, 29% protein, 12% fat) (hereinafter referred to as 'control') , The control group is divided into groups containing 0.5, 1, 2, 4 and 8% by weight of hot water extracts of pine needles, green tea and black tea prepared in [Example 1], and fed to each group 10 dogs for 5 weeks.

At this time, the total calorie intake was not significantly different between 0.5-4% by weight of the experimental group and the carbohydrate dietary control group, but 8% by weight of green tea and Black tea group was excluded from the experiment.

As a result, as shown in Figure 1a, pine needle extract was not significantly different from the control group in the 0.5% by weight diet containing group, 1, 2, 4, 8% by weight compared to the control group, respectively 4.1, 7.3, 10.1 The weight gain was decreased by 11.4%.

In addition, as shown in Figure 1b, the anti-obesity effect of the green tea extract was not significantly different from the control group in the 0.5% by weight diet containing group, but in the 1, 2, 4% by weight group, 3.4, 6.2, 6.5% weight gain decreased.

In addition, as shown in Figure 1c, the anti-obesity effect of the tea extract, there was no significant weight loss in the 0.5% by weight diet group, but in the 1, 2, 4% by weight group, 5.3, 7.2, Weight gain increased by 8.6%.

(2) Weight loss effect by mixing each extract:

TABLE 1

Table 1 above shows the theoretical weight loss effect assuming that each component independently has an addictive effect based on the effect of each extract of pine needles, green tea and black tea respectively on the weight.

Under these assumptions, the theoretical maximum effect is expected to have a weight loss effect when the diet of rats for 5 weeks is 8% by weight (4% by weight of pine needles + 2% by weight of green tea + 2% by weight of black tea). 23.5% decrease, dietary content is 7% by weight (4% by weight of pine needles + 2% by weight of green tea + 1% by weight of tea) 21.6% decrease, dietary content of 6% by weight (2% by weight of pine needles + 2% by weight of green tea + black tea) 2% by weight), 20.7% decrease in dietary content, 5% by weight (1% by weight of pine needles + 2% by weight of green tea + 2% by weight of black tea), 17.3% of decrease in dietary content, 4% by weight (2% by weight of pine needles + It is expected to decrease by 16% when 1% by weight of green tea + 1% by weight of black tea, and 12.6% when dietary content is 3% by weight (1% by weight of pine needles + 1% by weight of green tea + 1% by weight of black tea). The combinations were thought to be the most effective. However, the effect of 1% by weight of dietary content was decreased by 4.27% at 1% by weight of pine needles + 1% by weight of green tea + 1% by weight of black tea.

Based on this theoretical basis, 3% by weight (1% by weight of pine needles + 1% by weight of green tea + 1 tea by weight) of the mixture in the mixture corresponding to 1, 2, 4, and 8% by weight of each component having an anti-obesity effect %) More than 4, 8% by weight of the pine leaf, green tea and black tea extract mixture ratio was 2: 1: 1 in order to compare the mixture composition with a single administration group, the mixture diet content of 1, 2, 4, 8% by weight Was made.

In addition, Sprague-Dawley-based rats (about 50g) were mixed with a control group, and the hydrothermal extracts of pine needles, green tea and black tea prepared in [Example 1] were 2: 1: 1. The mixture diet was divided into 1, 2, 4 and 8% by weight containing groups, and 10 animals in each group were raised for 5 weeks.

In this case, no significant difference in total caloric intake was found between the experimental and control groups. That is, as shown in Figure 1d, the extract mixture was decreased by 8.2, 11.4, 14.6, 16.9%, respectively, compared to the control group in the 1, 2, 4, 8% by weight of the group, respectively, the greater the weight increase inhibitory effect was greater . In addition, Figure 1e is the pine leaf, green tea and black tea extracts 1, 2, 4, 8% by weight of the group showing the best weight increase inhibitory effect in each of the group, and the mixed group, dietary content 1, 2, 4, 8 In the wt% group, the mixed group showed a greater weight loss effect by 3.9, 4.1, 4.5, and 5.5%, respectively, than the single-treated control group, but only in the 2,4 wt% group.

Example 3 Experiment of Abdominal Fat Reduction in Rats

(1) Abdominal fat reduction effect of pine needles, green tea and black tea extract:

Abdominal fat of the same mice tested in (1) of [Example 2] was measured. Abdominal fat was measured using the rat abdominal aorta and after the epididymal and retroperitoneal fats were removed.

As a result, as shown in Figure 2a, pine needle extract in the 0.5, 1, 2, 4, 8% by weight group compared to the control group, respectively, 4.4, 11.1, 16.8, 28.5, 31.3% abdominal fat decreased but 0.5% by weight group Was not significant. In addition, Figure 2b shows the effect of the green tea extract on the abdominal fat, the amount of abdominal fat of 5.7, 9.8, 23.1, 25.7% in all 0.5, 1, 2, 4% by weight compared to the control group, respectively. In addition, Figure 2c shows the effect of the black tea extract, also 9, 14.3, 25.1 and 29.9% abdominal fat decreased in all 0.5, 1, 2, 4% by weight compared to the control group, respectively.

(2) abdominal fat reduction effect by mixing each extract:

The same mice as in (2) of [Example 2] were measured. As a result, as shown in Figure 2d, the extract mixture in the 1, 2, 4, 8% by weight group, respectively, 14.2, 29.6, 38, 39.7% abdominal fat decreased compared to the control group, respectively. In addition, Figure 2e is a pine leaf, green tea and black tea extract 1, 2, 4, 8% by weight compared to the group showing the best abdominal fat reduction effect in each of the groups, mixed in the 2, 4, 8% by weight group The abdominal fat reduction was more significant in the group by 4.5, 7.9, and 8.4% than the control group, respectively, and there was a 3% difference in the 1% by weight group, but it was not statistically significant.

[Example 4]: Experiment of increasing the variance of rats

(1) Increased variance effect of pine needles, green tea and black tea extract:

Again, the variance of the same mice tested in (1) of [Example 2] was measured. As a result, as shown in Figure 3a, pine needle extract increased by 5.4, 12, 21, 30, 33% variance in all 0.5, 1, 2, 4, 8% by weight compared to the control group, respectively. In addition, Figure 3b shows the effect of the green tea extract on the variance, 10, 25, 27, 28% variance was increased in all 0.5, 1, 2, 4% by weight compared to the control group. In addition, Figure 3c shows the effect of the black tea extract, and also in the 0.5, 1,2,4% by weight of the 12, 25, 29, 32% variance increased compared to the control, respectively.

(2) Variable increase effect by mixing each extract:

Also in the rat as in (2) of [Example 2] was measured in the subject, as a result, as shown in Figure 3d, the extract mixture in each of the 1, 2, 4, 8% by weight compared to the control group, respectively 23 , 31, 35 and 40% variance increased. In addition, Figure 4e is the pine leaf, green tea and black tea extract 1, 2, 4, 8% by weight compared to the group showing the best variance increase effect in each of the group, the mixed group, high variance in the mixed group 8% by weight There was no significant difference in the 1, 2 and 4 wt% group.

Example 5 Histologic Examination by Diet of Each Extract of Rat

In order to examine the side effects that may occur in the tissues of rats when the mixture of pine needles, green tea and black tea extracts is administered under the same conditions as in (2) of [Example 2], the brain, cerebellum, heart, liver and kidney Histologic findings were found in the small, small intestine, pancreas, and skeletal muscle, but no differences were found with the normal control.

In conclusion, the pine needle, green tea and black tea extract mixtures had a significantly higher weight loss and abdominal fat reduction effect when the same amounts were used in the diets containing 1, 2, 4 and 8% by weight. In addition, by effectively increasing the amount of variance could be estimated to have an excellent effect in the prevention and treatment of obesity.

In addition, it was estimated to be able to prevent and treat obesity and constipation while eating a normal meal without a special diet, because it is relatively less toxic than a large dose of a single component, chemically synthesized and sold. .

According to the composition of the present invention, not only can exhibit a higher obesity inhibitory effect when the mixed administration of these extracts than when administered alone by each hot water extract, but also decreases in weight gain and abdominal fat without reducing dietary intake. It is also suitable as an additive for health supplements because it can be used very effectively in the treatment and prevention of obesity and constipation by increasing the variance.

The present invention is to extract and produce a specific ingredient of a natural food in a predetermined ratio is harmless to the human body can exert an excellent effect in suppressing and preventing obesity or constipation symptoms without any side effects.

Claims (2)

Pine needle, green tea and black tea extract, characterized in that the mixing ratio of the pine needle extract, green tea extract and black tea extract extracted by hot water at a predetermined temperature are mixed in a weight ratio of 1 to 4: 1 to 4: 1 to 4 Composition for the treatment of obesity and constipation containing. The method of claim 1, The hot water is a composition for treating obesity and constipation containing pine needles, green tea and black tea extract as an active ingredient, characterized in that by heating the distilled water to 70 ~ 90 ℃ for a predetermined time.