KR20040048202A - A sterilizing agent for bean sprout or green bean sprout and a method for cultivating of bean sprout or green bean sprout using the same - Google Patents

Abstract

PURPOSE: A method of culturing seeds of bean sprouts or green bean sprouts with a disinfectant containing a quaternary ammonium chloride compound as main material is provided. The disinfectant is convenient to use and high in safety while having excellent controlling effects against spoilage-causing bacteria of bean sprouts and thus effective in improving the quality and productivity of bean sprouts. CONSTITUTION: The disinfectant for seeds of bean sprouts or green bean sprouts contains any one component or a mixture thereof selected from n-alkyldimethylbenzyl ammonium chloride of formula 1 and n-alkyldimethylethylbenzyl ammonium chloride of formula 2 in a ratio of 1:1 to 2:1. In formula, R is C12-18 or C12-14. To prevent spoilage of bean sprouts or green bean sprouts, seeds of bean sprouts or green bean sprouts are soaked in the disinfectant, or the cultivation vessel of bean sprouts or green bean sprouts is disinfected with the disinfectant.

Description

Soybean sprouts or host sprout seed disinfectants based on quaternary ammonium chloride compounds and methods for cultivating bean sprouts anti-corruption using same

The present invention relates to an antiseptic agent based on a quaternary ammonium chloride compound and a method for preventing rot of soybean sprouts or host sprouts using the same.

Soybean sprouts have been established as a representative national consumer food since it is a nutritious food that can be cultivated all year round and contains a large amount of aspartic acid, which helps supply vitamin C and natural biochemical functions in the human body. However, despite its rich nutritional value, it has been a long time since the annual pesticide sprouts case, like the annual event, has already lost the trust and faith of the people.

Bean sprouts are cultivated in closed rooms under high temperature, high humidity and light-blocking conditions, so the production of bean sprouts is prone to decay by fungal fungi and pathogenic bacteria. In addition, if the sprouts continue to rot, soybean sprout cultivation cultivation and the entire grower are easily contaminated with the causative agents, and in the absence of proper control methods, the sprouts are easily rotted, despite the excellent quality of the germination rate of the raw seeds. Infected, rotted, and turned into waste.

In order to prevent the rot of soybean sprouts, some growers may use ziorum hydration (trade name: Homai, Aventis Co.), kaboram powder (trade name: Vitajiram, Oriental Agrosa), mankoji hydration (trade name: Daisen M 45 ", agricultural Illegal farming of pesticides and other unacceptable fungicides, etc., has caused social problems unfortunately.The use of toxic fungicides that are harmful to the human body can easily prevent the decay of bean sprouts and host sprouts. This is a great threat to national health.

Soybean sprout decay is largely caused by the soybean sprout raw material seeds.

The causative agents of soybean sprouts can be classified into two types, fungi and bacteria. Fungi are known as Fusarium moniliforme, Fusarium oxysporum, and Fusarium solani. As a bacterium, Pseudomonas spp. Causes soybean seeds and cotyledon, rot, decay and germination. Erwinia Carotovora subsp. It has been reported that carotovora causes chorus , which inhibits the kidney of the hypocotyls of sprouts.

Unlike other crops, the environment in which bean sprouts are grown has cultivating characteristics such as dark conditions, high humidity due to frequent irrigation, and a closed cultivation space that is difficult to ventilate. The environment is known to have a significant impact.

Currently, bean sprouts are not allowed to be used for pesticides other than growth regulators, such as indole solution or BAC solution, and "carbendazime". "Thiabendazole". "Captan". All residue tolerance standards for pesticides such as "Tiram" are set to non-detection, so there is no way to control the corruption of soybean sprouts. Given the fact that more than 90% of the raw soybean sprouts are covered by imported soybeans, this is not only a huge foreign currency loss, but also causes serious environmental pollution.

The anti-corruption cultivation method was developed using antibacterial activity such as wood vinegar and chitosan. Seed sterilization was also used to prevent soybean sprout rot, and its antimicrobial activity was poor.

Since bean sprouts are grown in a short period of about 7 days, the treatment during the growing process is a high risk of drug residue. As mentioned above, the biggest cause of bean sprout rot is due to poor quality including the germination rate of raw soybeans. Therefore, soybean seed disinfection alone can control almost all decayed diseases of soybean sprouts. 1) It has good sterilizing power against soybean sprout decay bacteria. 2) It is safe to human body with low toxicity. 3) It has no problem of food safety due to low crop residue. 4) 5) It is urgent to develop a disinfectant disinfectant that is simple to use and inexpensive.

The present inventors intensively studied disinfectant disinfectants related to bean sprout cultivation satisfying the above-mentioned desirable conditions, and as a quaternary ammonium chloride compound, n-Alkyl dimethyl benzyl ammonium chloride and alkyldimethylethylbenzyl ammonium chloride ( N-Alkyl dimethyl ethylbenzyl ammonium chloride) alone or a mixture thereof was found to have an excellent control effect on the above-mentioned soybean sprouts rot causing bacteria through experiments of the actual sprouts growers.

The structural formulas of the two quaternary ammonium chloride compounds are shown in the following Chemical Formulas 1 and 2, respectively.

Wherein R is C _12-18 alkyl.

Wherein R is C _12-14 alkyl.

These quaternary ammonium chloride preparations, alkyldimethylbenzyl ammonium and alkyldimethylethylbenzyl ammonium, are colorless, odorless and non-irritating, so they are very soluble in water and alcohols, are convenient to use, and are very stable against acids, alkalis and heat. Very stable

The mixed formulation of the present invention has a wide range of activity and exhibits a very stable effect even in the presence of organics, and is widely used as a fungicide, algaecide, virus eradication agent (Virucide), etc. There is no example applied to sterilization and disinfection of the causative agent of the grower's environment.

The seed disinfectant of the present invention may contain an alkyl dimethyl benzyl ammonium chloride and an alkyl dimethyl ethyl benzyl ammonium chloride alone, or a mixture thereof. Each quaternary ammonium chloride formulation may also be in the form of a mixture of several compounds in which the alkyl substituent moiety is alkyl having different carbon numbers. For example, in the former, n-alkyl (C ₁₄ 60%, C ₁₆ 30%, C ₁₂ 5%, and C ₁₈ 5%) dimethylbenzyl ammonium chloride, as in the examples used in the examples below, n-alkyl (C ₁₂ 68%, C ₁₄ 32%) can be used in the form of dimethylethylbenzylammonium chloride.

When using these two themes in combination, the mixing ratio is not critical and the experimentally preferred ratio is approximately alkyldimethylbenzyl ammonium chloride to alkyldimethylethylbenzyl ammonium = 1 to 100: 1 to 1 to 100, more preferably Was a ratio of 1: 1 to 2: 1. In the following examples, the same amount of mixture was used.

In addition, the seed disinfectant of the present invention may optionally include ethyl alcohol or isopropyl alcohol as a dispersant to enhance the dispersing effect, in addition to the quaternary ammonium chloride component, in which case the amount of dispersant used is 0.5 to 5%. It is preferable. If it exceeds 5%, it is economically unfavorable, and if it is less than 0.5%, it is difficult to fully expect the desired dispersion effect.

The seed disinfectant of the present invention is first diluted 3 to 5 times and then used again by diluting 150 to 300 times. Soaked soybean seed or soybean sprout or host in the seed disinfectant of the present invention thus diluted. The herb cultivation vessel is used in such a manner as to disinfect with the disinfectant of the present invention. If the dilution factor is exceeded, it is difficult to expect a significant disinfection effect. If the immersion time is less than 2 hours, the disinfection effect is insufficient, and if more than 4 hours, side effects are concerned irrespective of the increase in disinfection effect such as seed bean retreat. At this time, the dispersant is used by the first dilution.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the scope of the present invention is not limited to these Examples.

Example 1

100 g and n of 68391-01-5 alkyl (60% C ₁₄ , 30% C ₁₆ , 5% C ₁₂ , 5% C ₁₈ ) dimethylbenzyl ammonium chloride (CAS No. 68391-01-5 Bulk Medicine & Pharmaceuticals) 100 g of alkyl- (68% C ₁₂ , 30% C ₁₄ ) dimethylethylbenzyl ammonium chloride (CAS No. 85409-23-0; Bulk Medicine & Pharmaceuticals) was added to 30 ml of ethyl alcohol as a dispersant and 770 ml of solvent as purified water. 1,000 ml of liquid sterilizer was prepared by uniformly mixing for 2 hours or more. 80 ml of the obtained liquid formulation is diluted in 20 liters of water (250 times liquid), and 20 kg of soybeans are immersed in the diluent for at least 2 hours, so that the chemical liquid is absorbed well, followed by cultivation in a growing room, in a dark room at 20 to 25 ° C. According to the cultivation method of the parallel treatment, it was grown for 7 days while sprinkling using an automatic sprinkler six times a day. The results obtained by repeating the results three times with randomized block design are shown in Table 1 below, with the morbidity and field investigation of 500 grains as the main investigation items. It was. In the present embodiment, the non-treatment group refers to a group that is treated only with growth regulators such as indolebi solution and Biei solution, and does not have a separate chemical treatment for preventing corruption. That is, in this control test, the same bean sprouts were treated with the same bean sprout raw material soybean sprout growth regulators in each treatment group, and all experiments were performed under the same cultivation conditions except seed disinfection treatment to prevent corruption. .

Drug Control Effect on Soybean Sprout Decay (Day 7) Test drug % Morbidity Weakness Growth 1 repetition 2 repetitions 3 repetitions n-alkyl (60% C ₁₄ , 30% C ₁₆ , 5% C ₁₂ , 5% C ₁₈ ) dimethylbenzyl ammonium chloride + n-alkyl (68% C ₁₂ , 30% C ₁₄ ) dimethylethylbenzylammonium 1 : 1 preparation 9.7 8.4 8.9 0 (no harm) Very good No treatment 32.2 34.7 32.8 - Browning phenomenon

As can be seen from the results of Example 1, the seed disinfectant based on n-alkyldimethylbenzyl ammonium chloride and n-alkyldimethylethylbenzyl ammonium chloride of the present invention has a very good control effect against soybean sprout rot and weakens growth. It does not occur at all and is useful as a seed disinfectant for controlling bean sprout rot.

Example 2

100 ml of the liquid preparation prepared in the same manner as in Example 1 was diluted in 20 liters of water (200 times liquid) and sterilized by a conventional method for washing bean sprouts. We also compared the effects of washing with household synthetic detergents, disinfection using lacx (sodium hypochlorite solution) and disinfection with boiling water disinfection. In order to get a clearer comparison, the same sprouts raw soybeans were added to each ash through the process of sterilization or washing using the above four methods by using soybean sprout cultivation soil that is heavily contaminated with the causative agent due to severe corruption during actual cultivation. It was cultivated according to the conventional method of cultivation using. As a result, by comparing the morbidity of soybean sprouts grown in sterilized or washed soybean sprouts as different drugs, the bactericidal effect against the decay-caused bacteria contaminated in the bean sprouts container is described in Table 2 below.

Soybean Sprout Bending Rate According to Disinfection Types of Soybean Sprout Cultivation Test drug Bean sprouts % Morbidity Time (20 bases) Treatment method n-alkyl (60% C ₁₄ , 30% C ₁₆ , 5% C ₁₂ , 5% C ₁₈ ) dimethylbenzyl ammonium chloride + n-alkyl (68% C ₁₂ , 30% C ₁₄ ) dimethylethylbenzylammonium 1 : 1 preparation 5.6 3 minutes Dipping after dilution Household Synthetic Detergent 27.4 20 minutes washing Lax (Sodium hypochlorite solution) 20.5 3 minutes Dipping after dilution Boiling water disinfection 5.4 30 minutes Immersion sterilization

From the results of Table 2, it can be seen that the sterilization effect of bean sprout cultivation base is greatly affected by the type of disinfectant used. In other words, when the same seed beans were grown under the same cultivation conditions, the sterilization of soybean sprout cultivation by boiling water disinfection was the most effective, but it required a lot of time and labor and safety precautions. It is true. On the contrary, the sterilization and washing by the mixed preparation of ammonium dimethylbenzyl ammonium chloride and alkyldimethylethylbenzyl ammonium chloride according to the present invention has not only the effect is almost equivalent to boiling water disinfection, but also is easy to use and effective sterilization and cleaning in a short time. It was found that it was possible to provide a very good effect.

As can be seen from the above results, the disinfectant disinfectant based on the sole or mixture of the alkyl dimethylbenzyl ammonium chloride and the alkyl dimethylethylbenzyl ammonium chloride of the quaternary ammonium chloride compound of the present invention, soybean sprout seeds as well as cultivation It has a very good control effect against soybean sprout decaying agents that contaminate etc. It is not only able to effectively control the corruption of bean sprouts when growing bean sprouts, but also easy to use and excellent in safety. It is expected to have a great effect on the quality improvement and production of bean sprouts. In addition, the seed disinfectant of the present invention and the disinfection method using the same can be used for bean sprouts and growth patterns, host sprouts similar in cultivation conditions.

Claims (4)

A seed disinfectant of bean sprouts or host sprouts containing any one selected from n-alkyldimethylbenzyl ammonium chloride of formula 1 and n-alkyldimethylethylbenzyl ammonium chloride of formula 2 or a mixture thereof as a subject. [Formula 1] Wherein R is C _12-18 alkyl. [Formula 2] Wherein R is C _12-14 alkyl. The compound of formula 1 is n-alkyl (60% C ₁₄ , 30% C ₁₆ , 5% C ₁₂ , 5% C ₁₈ ) dimethylbenzyl ammonium chloride, and the compound of formula 2 is n-alkyl ( 68% C ₁₂ , 30% C ₁₄ ) Seed disinfectant which is dimethylethylbenzyl ammonium chloride. The liquid seed disinfectant according to claim 2, wherein the n-alkyldimethylbenzyl ammonium chloride and n-alkyldimethylethylbenzyl ammonium chloride are mixed in a ratio of 1: 1 to 2: 1. The anti-corruption cultivation method of bean sprouts or host sprouts, which is immersed in the seed disinfectant according to any one of claims 1 to 3 or sterilizing the bean sprouts or host sprouts cultivation container.