KR20040046678A - -- Novel Microorganism and Process for the production of Poly-beta-Hydroxy Butyric Acid PHB by Employing the Microorganism - Google Patents

-- Novel Microorganism and Process for the production of Poly-beta-Hydroxy Butyric Acid PHB by Employing the Microorganism Download PDF

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Publication number
KR20040046678A
KR20040046678A KR1020020074673A KR20020074673A KR20040046678A KR 20040046678 A KR20040046678 A KR 20040046678A KR 1020020074673 A KR1020020074673 A KR 1020020074673A KR 20020074673 A KR20020074673 A KR 20020074673A KR 20040046678 A KR20040046678 A KR 20040046678A
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KR
South Korea
Prior art keywords
microorganism
phb
beta
ralstonia eutropha
poly
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KR1020020074673A
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Korean (ko)
Inventor
김갑진
고명석
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(주) 켐포트
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Priority to KR1020020074673A priority Critical patent/KR20040046678A/en
Publication of KR20040046678A publication Critical patent/KR20040046678A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE: A microorganism producing poly-beta-hydroxy butyric acid (PHB) and a process for producing PHB using the same are provided, thereby maximizing the growth rate of microorganism and PHB accumulation, and minimizing physical property difference between lots. CONSTITUTION: The microorganism Ralstonia eutropha(KCTC 10351BP) produces and accumulates 80% or more of poly-beta-hydroxy butyric acid (PHB) in a medium containing high fructose syrup as a sole carbon source, wherein Ralstonia eutropha(KCTC 10351BP) is produced by irradiating UV(ultraviolet) to Ralstonia eutropha strain ATCC 17697, spreading the UV treated microorganism on agar medium with high C/N ratio, culturing the microorganism for 48 to 72 hours, and isolating milky white colonies grown and selecting one microorganism capable of using glucose and accumulates PHB. The process for producing PHA(polyhydroxy alkanoate) comprises fed-batch fermenting Ralstonia eutropha(KCTC 10351BP) in a medium containing ammonia water as a nitrogen source and a pH regulating agent, wherein the carbon source is glucose, fructose and high fructose syrup; and the C/N ratio is maintained at 15 to 25 up to 48 hours during the fed-batch fermentation, and the C/N ratio is maintained at 25 to 35 and the pH regulating agent is substituted by sodium hydroxide after 48 hours.
KR1020020074673A 2002-11-28 2002-11-28 -- Novel Microorganism and Process for the production of Poly-beta-Hydroxy Butyric Acid PHB by Employing the Microorganism KR20040046678A (en)

Priority Applications (1)

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KR1020020074673A KR20040046678A (en) 2002-11-28 2002-11-28 -- Novel Microorganism and Process for the production of Poly-beta-Hydroxy Butyric Acid PHB by Employing the Microorganism

Applications Claiming Priority (1)

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KR1020020074673A KR20040046678A (en) 2002-11-28 2002-11-28 -- Novel Microorganism and Process for the production of Poly-beta-Hydroxy Butyric Acid PHB by Employing the Microorganism

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KR20040046678A true KR20040046678A (en) 2004-06-05

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110392737A (en) * 2016-12-30 2019-10-29 株式会社三养社 With the method for producing psicose epimerase micro-organisms psicose

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR920019943A (en) * 1991-04-09 1992-11-20 이상운 Poly-β-hydroxy alkanoate (PHA) having a copolymer structure of poly-β-hydroxy butyrate (PHB) and poly-β-hydroxy butyrate / poly-β-hydroxy valeric acid (PHB / PHV) Manufacturing method
KR920021705A (en) * 1991-05-22 1992-12-18 이영하 Microorganisms of the genus Alkalizenes synthesizing PHA
WO1998033931A1 (en) * 1997-02-04 1998-08-06 Buna Sow Leuna Olefinverbund Gmbh Microbial method for producing polyhydroxy alkanoates
US6395520B1 (en) * 1998-04-30 2002-05-28 Ufz-Umweltforschungszentrum Leipsig-Halle Gmbh Method for the continuous microbiological production of polyhydroxy butyric acid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR920019943A (en) * 1991-04-09 1992-11-20 이상운 Poly-β-hydroxy alkanoate (PHA) having a copolymer structure of poly-β-hydroxy butyrate (PHB) and poly-β-hydroxy butyrate / poly-β-hydroxy valeric acid (PHB / PHV) Manufacturing method
KR920021705A (en) * 1991-05-22 1992-12-18 이영하 Microorganisms of the genus Alkalizenes synthesizing PHA
WO1998033931A1 (en) * 1997-02-04 1998-08-06 Buna Sow Leuna Olefinverbund Gmbh Microbial method for producing polyhydroxy alkanoates
US6395520B1 (en) * 1998-04-30 2002-05-28 Ufz-Umweltforschungszentrum Leipsig-Halle Gmbh Method for the continuous microbiological production of polyhydroxy butyric acid

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Appl Microbiol Biotechnol. 2002 Apr;58(5):675-8. Epub 2002 Feb 14 *
The Journal of microbiology , March 2001 , 37(1) , 92-99 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110392737A (en) * 2016-12-30 2019-10-29 株式会社三养社 With the method for producing psicose epimerase micro-organisms psicose
CN110392737B (en) * 2016-12-30 2023-05-05 株式会社三养社 Method for producing psicose by using psicose-producing epimerase microorganism

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